CN114807260A - Enzymatic synthesis mode of D-tryptophan - Google Patents
Enzymatic synthesis mode of D-tryptophan Download PDFInfo
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- CN114807260A CN114807260A CN202210641477.2A CN202210641477A CN114807260A CN 114807260 A CN114807260 A CN 114807260A CN 202210641477 A CN202210641477 A CN 202210641477A CN 114807260 A CN114807260 A CN 114807260A
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- 229930182827 D-tryptophan Natural products 0.000 title claims abstract description 33
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 title claims abstract description 33
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 18
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 17
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 57
- 239000000463 material Substances 0.000 claims abstract description 47
- 238000010438 heat treatment Methods 0.000 claims abstract description 33
- 238000006243 chemical reaction Methods 0.000 claims abstract description 25
- 230000007246 mechanism Effects 0.000 claims abstract description 21
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 238000001816 cooling Methods 0.000 claims abstract description 10
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 230000006340 racemization Effects 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 5
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- QIVBCDIJIAJPQS-UHFFFAOYSA-N tryptophan Chemical compound C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 239000010413 mother solution Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 5
- 238000001308 synthesis method Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000007791 liquid phase Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims 7
- 238000007789 sealing Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 239000000110 cooling liquid Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KZZWTVCKSGRXMY-UHFFFAOYSA-N C(CC)(=O)O.NC=1NC2=CC=CC=C2C1 Chemical compound C(CC)(=O)O.NC=1NC2=CC=CC=C2C1 KZZWTVCKSGRXMY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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Abstract
The invention relates to the technical field of D-tryptophan synthesis, in particular to an enzymatic synthesis mode of D-tryptophan, which comprises the steps of putting L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction; and (3) carrying out gradient cooling on the material obtained in the step S1, wherein a gradient is formed at every 5 ℃, and carrying out heat preservation reaction for a certain time at the corresponding gradient temperature. After materials are added into the reaction kettle, the first motor is started to drive the inner shaft to rotate, the stirring pipe rotates along with the inner shaft, the first gear at the end part of the stirring pipe is meshed with the annular rack, so that the stirring pipe rotates, the stirring pipe is uniformly provided with a plurality of extending pieces, and the materials are stirred by the rotating of the extending pieces, so that the materials are mixed more fully, and the stirring efficiency is improved; evenly be provided with many heating rods in the inside of the jar body, inside the heating rod extended into the material, heating cooperation rabbling mechanism efficiency was higher.
Description
Technical Field
The invention relates to the technical field of D-tryptophan synthesis, in particular to an enzymatic synthesis mode of D-tryptophan.
Background
D-tryptophan, also known as aminoindole propionic acid, is a white or off-white to slightly yellow crystalline powder, has no odor and sweet taste, is slightly soluble in water, is insoluble in genetics, is soluble in olefine acid or olefine base, develops color when illuminated for a long time, and produces a small amount of indole together with water. D-tryptophan is an important amino acid necessary for human bodies and widely applied to medical and food purposes, such as amino acid transfusion, comprehensive amino acid preparation, pregnant woman nutritional supplement, special milk powder for infants, food additive and the like.
The enzymatic synthesis method is one of the common D-tryptophan synthesis methods, and during the operation of the conventional enzymatic synthesis method, the L-tryptophan and a solvent are firstly put into a reaction kettle, then a racemization agent is put into the reaction kettle, the temperature is raised to 40 ℃, the reaction kettle is stirred for 0.5h, then a resolving agent is added, the temperature is raised to 95 ℃ at the same time, and the temperature is kept for 24 h; then carrying out temperature control reaction on the mixture, and carrying out gradient temperature reduction on the mixture; then centrifuging the materials, finally washing the materials by using methanol, and centrifugally drying the materials; wherein, the material can be placed for a long time in reation kettle, and current reation kettle only possesses simple stirring and heating function, and only inside several puddlers rotate when stirring the reactant, stir inadequately, can only increase the churning time and improve the effect, in addition to heating the mixture or, during the cooling, current reation kettle alternating temperature speed is slow, and this all can greatly reduced production efficiency, need improve.
Disclosure of Invention
The present invention is directed to an enzymatic synthesis method of D-tryptophan, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: an enzymatic synthesis mode of D-tryptophan, which comprises the following steps: s1: putting L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction;
s2: carrying out gradient cooling on the material obtained in the step S1, wherein a gradient is formed at every 5 ℃, and carrying out heat preservation reaction for a certain time at the corresponding gradient temperature;
s3: centrifuging the material obtained in the step S2 to obtain a mixture of a solid D-tryptophan and a resolving agent complex, wherein the liquid phase is a mother solution of DL-tryptophan, the solid is subjected to complex resolution to obtain D-tryptophan and recover the resolving agent, and the mother solution of DL-tryptophan is subjected to pH =2-3 adjustment by HCl solution to recover DL-tryptophan;
s4: washing the D-tryptophan obtained in the step S3 with methanol once, centrifuging after washing, and drying the D-tryptophan obtained after centrifuging.
Preferably, the reaction kettle device is including the mounting bracket, the mounting bracket is "Contraband" type structure, rotates on the mounting bracket and installs a jar body, install first motor on the bottom surface of the jar body, the output of first motor is connected with interior axle, interior axle runs through the bottom surface of the jar body, interior epaxial multi-functional rabbling mechanism that is provided with, be provided with the bulge loop on the lateral wall of the jar body, the inside of bulge loop is provided with annular rack, interior change is installed to the bulge loop internal rotation, be connected with on the both sides outer wall of bulge loop and annotate liquid pipe and fluid-discharge tube, install the heating rod on the middle part inside wall of the jar body, the below of the jar body is provided with the guiding mechanism of being convenient for empty the material.
Preferably, the top of the jar body is provided with the charge door, is provided with the erection column on the middle lateral wall of the jar body, and the erection column runs through the both sides wall of mounting bracket, and erection column and mounting bracket rotate to be connected, are provided with the discharge gate on the bottom lateral wall of the jar body, are provided with the solenoid valve on the discharge gate.
Preferably, the stirring mechanism comprises two stirring pipes, the stirring pipes penetrate through the inner shaft and are rotatably connected with the inner shaft, two ends of each stirring pipe penetrate through the inner rotating ring and extend into the convex ring, the stirring pipes are rotatably connected with the inner rotating ring, one end of each stirring pipe is provided with a first gear, and the first gear is meshed with the annular rack.
Preferably, evenly be provided with the several on the agitator pipe and extend the piece, extend the hollow flat pipe of U type, the inside and the agitator pipe of extension piece are linked together.
Preferably, the heating rods are arranged in a ring shape, and the number of the heating rods is 8-15.
Preferably, a temperature sensor is mounted on the inner side wall of the tank body.
Preferably, the adjusting mechanism comprises two vertical plates fixed on the bottom surface of the tank body, a bottom plate is installed between the two vertical plates, the bottom plate is an arc-shaped plate, and a rack is arranged on the bottom surface of the bottom plate.
Preferably, guiding mechanism is still including rotating the dwang of installing on the mounting bracket, is provided with the second gear on the dwang, and second gear and rack toothing are connected, install the second motor on the lateral wall of mounting bracket, and the output shaft and the dwang of second motor are connected.
Compared with the prior art, the invention has the beneficial effects that:
1. after materials are added into the reaction kettle, the first motor is started to drive the inner shaft to rotate, so that the stirring pipe rotates along with the inner shaft, the first gear at the end part of the stirring pipe is meshed with the annular rack, so that the stirring pipe rotates, the stirring pipe is uniformly provided with a plurality of extending pieces, and the extending pieces rotate to stir the materials, so that the materials are mixed more fully, and the stirring efficiency is improved;
2. a plurality of heating rods are uniformly arranged in the tank body, the heating rods extend into the material, the heating and matching stirring mechanism has higher efficiency, when the tank is cooled, cooling liquid is injected into the convex ring from the liquid injection pipe, and then the cooling liquid enters the stirring pipe and the extension piece, so that the effective cooling contact surface of the material is larger, the cooling efficiency is greatly improved, and the production efficiency is improved;
3. after the reaction, the solenoid valve on the discharge gate is opened and can be discharged the material, because of being provided with the guiding mechanism who is convenient for empty the material, the second motor starts to drive dwang and second gear revolve, and second gear and rack toothing are connected, can adjust the angle of the jar body for jar body slope certain angle can prevent to have a large amount of materials to remain in the bottom surface of the jar body when unloading, prevents extravagant material.
Drawings
FIG. 1 is a schematic view of the apparatus according to the present invention;
FIG. 2 is a view showing the structure of the tube of the present invention;
FIG. 3 is a schematic view of the apparatus of the present invention;
fig. 4 is an enlarged view of a portion a in fig. 3.
In the figure: the device comprises a mounting frame 1, a tank body 2, a charging opening 201, a mounting column 202, a discharging opening 203, a first motor 3, an inner shaft 4, a stirring pipe 5, an extension piece 6, a convex ring 7, an inner rotary ring 8, a first gear 9, an annular rack 10, a liquid injection pipe 11, a liquid discharge pipe 12, a heating rod 13, a temperature sensor 14, a vertical plate 15, a bottom plate 16, a rack 17, a rotating rod 18, a second motor 19 and a second gear 20.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 4, the present invention provides a technical solution: an enzymatic synthesis mode of D-tryptophan, which comprises the following steps:
s1: putting L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction;
s2: carrying out gradient cooling on the material obtained in the step S1, wherein a gradient is formed at every 5 ℃, and carrying out heat preservation reaction for a certain time at the corresponding gradient temperature;
s3: centrifuging the material obtained in the step S2 to obtain a mixture of a solid D-tryptophan and a resolving agent complex, wherein the liquid phase is a mother solution of DL-tryptophan, the solid is subjected to complex resolving to obtain D-tryptophan and the resolving agent is recovered, and the mother solution of DL-tryptophan is subjected to pH =2-3 adjustment by HCl solution to recover DL-tryptophan;
s4: washing the D-tryptophan obtained in the step S3 with methanol once, centrifuging after washing, and drying the D-tryptophan obtained after centrifuging.
The reaction kettle device comprises a mounting frame 1, the mounting frame 1 is of an Contraband type structure, a tank body 2 is rotatably mounted on the mounting frame 1, the top of the tank body 2 is provided with a feed inlet 201, a sealing cover is arranged on the feed inlet 201, one end of the sealing cover is hinged to be mounted, the other end of the sealing cover is connected through a buckle, a mounting column 202 is arranged on the middle side wall of the tank body 2, the mounting column 202 penetrates through two side walls of the mounting frame 1, the mounting column 202 is rotatably connected with the mounting frame 1, a discharge port 203 is formed in the bottom side wall of the tank body 2, and an electromagnetic valve is arranged on the discharge port 203.
The bottom surface of the tank body 2 is provided with a first motor 3, the output end of the first motor 3 is connected with an inner shaft 4, the inner shaft 4 penetrates through the bottom surface of the tank body 2, the inner shaft 4 is provided with a multifunctional stirring mechanism, the stirring mechanism comprises two stirring pipes 5, the stirring pipes 5 penetrate through the inner shaft 4, the stirring pipes 5 are rotatably connected with the inner shaft 4, two ends of each stirring pipe 5 penetrate through an inner rotating ring 8 and extend into a convex ring 7, the stirring pipes 5 are rotatably connected with the inner rotating ring 8, the stirring pipes 5 and the inner rotating ring 8 can be connected through sealing bearings, one end of each stirring pipe 5 is provided with a first gear 9, the first gear 9 is meshed with a circular rack 10, the stirring pipes 5 are uniformly provided with a plurality of extending pieces 6, each extending piece 6 is a U-shaped hollow flat pipe, and the interiors of the extending pieces 6 are communicated with the stirring pipes 5; after the material added reation kettle, axle 4 rotated in 3 starts the drive of first motor, and then made stirred tube 5 rotate thereupon, and the first gear 9 and the annular rack 10 meshing of stirred tube 5 tip are connected, and then make stirred tube 5 self rotate, and stirred tube 5 evenly is provided with a plurality of extension pieces 6, and extension piece 6 rotates and also stirs the material for the material mixes more fully, thereby has improved stirring efficiency.
Be provided with two rings of bulge loops 7 on the lateral wall of the jar body 2, the inside of bulge loop 7 is provided with annular rack 10, interior change 8 is installed to the bulge loop 7 internal rotation, interior change 8 embedding bulge loops 7 in, can be provided with the sealing ring between interior change 8 and the bulge loop 7 junction, prevent that the material from leaking, be connected with on the both sides outer wall of bulge loop 7 and annotate liquid pipe 11 and fluid-discharge tube 12, install heating rod 13 on the middle part inside wall of the jar body 2, heating rod 13 is the annular setting, the number of piles that heating rod 13 was provided with is decided according to the equipment size, every layer of heating rod 13's quantity is 8-15, heating rod 13 extends into in the material, many heating rod 13 heat jointly, can improve heating efficiency greatly, install temperature sensor 14 on the inside wall of the jar body 2, temperature sensor 14 is used for the temperature of the inside material of real-time supervision. When the cooling, the coolant liquid is injected into the inside of bulge loop 7 from annotating liquid pipe 11, then the coolant liquid enters into stirring pipe 5, extends 6 inside, and the effective cooling contact surface of material is bigger, has improved cooling efficiency greatly to production efficiency has been improved.
An adjusting mechanism convenient for dumping materials is arranged below the tank body 2, the adjusting mechanism comprises two vertical plates 15 fixed on the bottom surface of the tank body 2, a bottom plate 16 is arranged between the two vertical plates 15, the bottom plate 16 is an arc-shaped plate, a rack 17 is arranged on the bottom surface of the bottom plate 16, the adjusting mechanism also comprises a rotating rod 18 rotatably arranged on the mounting frame 1, a second gear 20 is arranged on the rotating rod 18, the second gear 20 is meshed with the rack 17, a second motor 19 is arranged on the outer side wall of the mounting frame 1, and an output shaft of the second motor 19 is connected with the rotating rod 18; after the reaction, the electromagnetic valve on the discharge port 203 is opened to discharge the materials, and the second motor 19 is started to drive the rotating rod 18 and the second gear 20 to rotate due to the arrangement of the adjusting mechanism which is convenient for dumping the materials, and the second gear 20 is meshed with the rack 17 to be connected, so that the angle of the tank body 2 can be adjusted, the tank body 2 is inclined at a certain angle, a large amount of materials can be prevented from being remained on the bottom surface of the tank body 2 during unloading, and the materials can be prevented from being wasted.
When the device is used, after materials are added into a reaction kettle, the first motor 3 is started to drive the inner shaft 4 to rotate, so that the stirring pipe 5 rotates, the first gear 9 at the end part of the stirring pipe 5 is meshed and connected with the annular rack 10, the stirring pipe 5 rotates, the stirring pipe 5 is uniformly provided with a plurality of extending pieces 6, and the extending pieces 6 rotate to stir the materials, so that the materials are mixed more fully, and the stirring efficiency is improved; a plurality of heating rods 13 are uniformly arranged in the tank body 2, the heating rods 13 extend into the material, the heating and matching stirring mechanism has higher efficiency, when the temperature is reduced, cooling liquid is injected into the convex ring 7 from the liquid injection pipe 11, then the cooling liquid enters the stirring pipe 5 and the extension piece 6, the effective temperature reduction contact surface of the material is larger, the temperature reduction efficiency is greatly improved, and the production efficiency is improved; after the reaction, the solenoid valve on the discharge gate 203 is opened and can be discharged the material, because of being provided with the guiding mechanism who is convenient for empty the material, second motor 19 starts to drive dwang 18 and second gear 20 and rotates, and second gear 20 and rack 17 meshing are connected, can adjust the angle of the jar body 2 for jar body 2 slopes certain angle, can prevent to have a large amount of materials to remain in jar body 2's bottom surface when unloading, prevents extravagant material.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. An enzymatic synthesis mode of D-tryptophan is characterized in that: the synthesis method comprises the following steps:
s1: putting L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction;
s2: carrying out gradient cooling on the material obtained in the step S1, wherein a gradient is formed at every 5 ℃, and carrying out heat preservation reaction for a certain time at the corresponding gradient temperature;
s3: centrifuging the material obtained in the step S2 to obtain a mixture of a solid D-tryptophan and a resolving agent complex, wherein the liquid phase is a mother solution of DL-tryptophan, the solid is subjected to complex resolution to obtain D-tryptophan and recover the resolving agent, and the mother solution of DL-tryptophan is subjected to pH =2-3 adjustment by HCl solution to recover DL-tryptophan;
s4: washing the D-tryptophan obtained in the step S3 with methanol once, centrifuging after washing, and drying the D-tryptophan obtained after centrifuging.
2. The enzymatic synthesis of D-tryptophan according to claim 1, wherein: the reaction kettle device comprises a mounting rack (1), the mounting rack (1) is of an Contraband-shaped structure, a tank body (2) is rotatably arranged on the mounting rack (1), a first motor (3) is arranged on the bottom surface of the tank body (2), the output end of the first motor (3) is connected with an inner shaft (4), the inner shaft (4) penetrates through the bottom surface of the tank body (2), the inner shaft (4) is provided with a multifunctional stirring mechanism, a convex ring (7) is arranged on the side wall of the tank body (2), an annular rack (10) is arranged inside the convex ring (7), an inner rotating ring (8) is rotatably arranged in the convex ring (7), the outer walls of two sides of the convex ring (7) are connected with a liquid injection pipe (11) and a liquid discharge pipe (12), install heating rod (13) on the middle part inside wall of the jar body (2), the below of the jar body (2) is provided with the guiding mechanism of being convenient for empty the material.
3. The enzymatic synthesis of D-tryptophan according to claim 2, wherein: the top of the tank body (2) is provided with a feed inlet (201), the middle side wall of the tank body (2) is provided with an installation column (202), the installation column (202) runs through the two side walls of the installation frame (1), the installation column (202) is rotatably connected with the installation frame (1), the bottom side wall of the tank body (2) is provided with a discharge port (203), and the discharge port (203) is provided with an electromagnetic valve.
4. The enzymatic synthesis of D-tryptophan according to claim 2, wherein: the stirring mechanism comprises two stirring pipes (5), wherein the stirring pipes (5) penetrate through an inner shaft (4), the stirring pipes (5) are rotatably connected with the inner shaft (4), two ends of each stirring pipe (5) penetrate through an inner rotating ring (8) and extend into a convex ring (7), the stirring pipes (5) are rotatably connected with the inner rotating ring (8), one end of each stirring pipe (5) is provided with a first gear (9), and the first gear (9) is meshed with an annular rack (10) to be connected.
5. The enzymatic synthesis of D-tryptophan according to claim 4, wherein: evenly be provided with several extension (6) on stirred tube (5), extension (6) are the hollow flat pipe of U type, and the inside and stirred tube (5) of extension (6) are linked together.
6. The enzymatic synthesis of D-tryptophan according to claim 5, wherein: the heating rods (13) are arranged in an annular shape, and the number of the heating rods (13) is 8-15.
7. The enzymatic synthesis of D-tryptophan according to claim 2, wherein: and a temperature sensor (14) is arranged on the inner side wall of the tank body (2).
8. The enzymatic synthesis of D-tryptophan according to claim 2, wherein: the adjusting mechanism comprises two vertical plates (15) fixed on the bottom surface of the tank body (2), a bottom plate (16) is installed between the two vertical plates (15), the bottom plate (16) is an arc-shaped plate, and a rack (17) is arranged on the bottom surface of the bottom plate (16).
9. The enzymatic synthesis of D-tryptophan according to claim 2, wherein: the adjusting mechanism further comprises a rotating rod (18) rotatably installed on the installation frame (1), a second gear (20) is arranged on the rotating rod (18), the second gear (20) is meshed with the rack (17) to be connected, a second motor (19) is installed on the outer side wall of the installation frame (1), and an output shaft of the second motor (19) is connected with the rotating rod (18).
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|---|---|---|---|---|
| JP2008061642A (en) * | 2006-08-10 | 2008-03-21 | Toyobo Co Ltd | Method for producing d-amino acid |
| CN103342676A (en) * | 2013-07-24 | 2013-10-09 | 马鞍山德鸿生物技术有限公司 | Method for synthetizing D-tryptophan |
| CN108103137A (en) * | 2017-09-15 | 2018-06-01 | 南通紫琅生物医药科技有限公司 | A kind of new process of chemical method and Production by Enzymes D-trp |
| CN208308870U (en) * | 2018-04-16 | 2019-01-01 | 长兴制药股份有限公司 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
| CN215996632U (en) * | 2021-07-19 | 2022-03-11 | 连云港康乐药业有限公司 | Acylation reaction tank with heating and cooling functions |
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2022
- 2022-06-08 CN CN202210641477.2A patent/CN114807260B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008061642A (en) * | 2006-08-10 | 2008-03-21 | Toyobo Co Ltd | Method for producing d-amino acid |
| CN103342676A (en) * | 2013-07-24 | 2013-10-09 | 马鞍山德鸿生物技术有限公司 | Method for synthetizing D-tryptophan |
| CN108103137A (en) * | 2017-09-15 | 2018-06-01 | 南通紫琅生物医药科技有限公司 | A kind of new process of chemical method and Production by Enzymes D-trp |
| CN208308870U (en) * | 2018-04-16 | 2019-01-01 | 长兴制药股份有限公司 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
| CN215996632U (en) * | 2021-07-19 | 2022-03-11 | 连云港康乐药业有限公司 | Acylation reaction tank with heating and cooling functions |
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