CN114807260B - Enzymatic synthesis mode of D-tryptophan - Google Patents
Enzymatic synthesis mode of D-tryptophan Download PDFInfo
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- CN114807260B CN114807260B CN202210641477.2A CN202210641477A CN114807260B CN 114807260 B CN114807260 B CN 114807260B CN 202210641477 A CN202210641477 A CN 202210641477A CN 114807260 B CN114807260 B CN 114807260B
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- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 13
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 13
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 12
- 229930182827 D-tryptophan Natural products 0.000 title abstract description 20
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 title abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 67
- 239000000463 material Substances 0.000 claims abstract description 44
- 238000010438 heat treatment Methods 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 5
- 238000001308 synthesis method Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 abstract description 12
- 239000003795 chemical substances by application Substances 0.000 abstract description 11
- 238000004321 preservation Methods 0.000 abstract description 6
- 239000002904 solvent Substances 0.000 abstract description 4
- 230000006340 racemization Effects 0.000 abstract description 3
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 239000000203 mixture Substances 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 239000000110 cooling liquid Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000010413 mother solution Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KZZWTVCKSGRXMY-UHFFFAOYSA-N C(CC)(=O)O.NC=1NC2=CC=CC=C2C1 Chemical compound C(CC)(=O)O.NC=1NC2=CC=CC=C2C1 KZZWTVCKSGRXMY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Thermal Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Accessories For Mixers (AREA)
- Mixers Of The Rotary Stirring Type (AREA)
Abstract
The invention relates to the technical field of D-tryptophan synthesis, in particular to an enzymatic synthesis mode of D-tryptophan, which comprises the steps of placing L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction; and (3) carrying out gradient cooling on the material obtained in the step (S1), wherein each 5 ℃ is a gradient, and carrying out heat preservation reaction for a certain time at the temperature of the corresponding gradient. When materials are added into the reaction kettle, the first motor is started to drive the inner shaft to rotate, the stirring pipe rotates along with the inner shaft, the first gear at the end part of the stirring pipe is meshed with the annular rack, so that the stirring pipe rotates, a plurality of extending pieces are uniformly arranged on the stirring pipe, the materials are stirred by the extending pieces, and the materials are mixed more fully, so that the stirring efficiency is improved; a plurality of heating rods are uniformly arranged in the tank body, the heating rods extend into the material, and the efficiency of the heating and stirring mechanism is higher.
Description
Technical Field
The invention relates to the technical field of synthesis of D-tryptophan, in particular to an enzymatic synthesis mode of D-tryptophan.
Background
D-tryptophan, also known as aminoindole propionic acid, is a white or off-white to yellowish crystalline powder, odorless and sweet, slightly soluble in water, insoluble in genetics, soluble in olefine acid or olefine base, colored when illuminated for a long time, and co-produced a small amount of indole with water. D-tryptophan is an important amino acid essential to human body and is widely applied to medical treatment and food application, such as amino acid transfusion, comprehensive amino acid preparation, nutrition supplement for pregnant women, special milk powder for infants, food additive and the like.
The enzymatic synthesis is a common method in the existing D-tryptophan synthesis method, when the existing enzymatic synthesis method works, L-tryptophan and a solvent are firstly put into a reaction kettle, then racemizer is put into the reaction kettle, the temperature is raised to 40 ℃, the stirring is carried out for 0.5h, then resolving agent is added, and meanwhile the temperature is raised to 95 ℃, and the temperature is kept for 24h; then carrying out temperature control reaction on the mixture, and carrying out gradient cooling on the mixture; centrifuging the materials, washing with methanol, centrifuging and drying; wherein, the material can be placed for a long time in reation kettle, and current reation kettle only possesses simple stirring and heating function, and inside only several puddler rotates when stirring the reactant, and the stirring is insufficient, can only increase stirring time and improve the effect, and when heating or cooling to the mixture, current reation kettle alternating temperature speed is slow moreover, and this all can greatly reduced production efficiency, needs to improve.
Disclosure of Invention
The invention aims to provide an enzymatic synthesis method of D-tryptophan, which aims to solve the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: an enzymatic synthesis of D-tryptophan, the synthesis comprising the steps of: s1: placing L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction;
s2: carrying out gradient cooling on the material obtained in the step S1, wherein each 5 ℃ is a gradient, and carrying out heat preservation reaction for a certain time at the temperature of the corresponding gradient;
s3: centrifuging the material obtained in the step S2 to obtain a mixture of D-tryptophan as a solid and a complex of a resolving agent, wherein a liquid phase is a mother solution of DL-tryptophan, D-tryptophan is obtained by decomplexing the solid and the resolving agent is recovered, and the mother solution of DL-tryptophan is recovered by adjusting pH=2-3 with HCl solution;
s4: and (3) washing the D-tryptophan obtained in the step (S3) with methanol once, performing centrifugal operation after washing, and drying the D-tryptophan obtained after centrifugal operation.
Preferably, the reaction kettle device comprises a mounting rack, the mounting rack is "" type structure, rotates on the mounting rack and installs the jar body, install first motor on the bottom surface of the jar body, the output of first motor is connected with interior axle, interior axle runs through the bottom surface of the jar body, be provided with multi-functional rabbling mechanism in the interior epaxial, be provided with the bulge loop on the lateral wall of the jar body, the inside of bulge loop is provided with annular rack, the inner swivel is installed to the bulge loop rotation, be connected with notes liquid pipe and fluid-discharge tube on the both sides outer wall of bulge loop, install the heating rod on the middle part inside wall of the jar body, the below of the jar body is provided with the guiding mechanism of being convenient for empty material.
Preferably, the top of the tank body is provided with the charge door, is provided with the erection column on the middle lateral wall of the tank body, and the erection column runs through the both sides wall of mounting bracket, and erection column and mounting bracket rotate to be connected, are provided with the discharge gate on the bottom lateral wall of the tank body, are provided with the solenoid valve on the discharge gate.
Preferably, the stirring mechanism comprises two stirring pipes, the stirring pipes penetrate through the inner shaft, the stirring pipes are connected with the inner shaft in a rotating way, two ends of each stirring pipe penetrate through the inner rotating ring and extend into the protruding ring, the stirring pipes are connected with the inner rotating ring in a rotating way, a first gear is arranged at one end of each stirring pipe, and the first gear is connected with the annular rack in a meshed way.
Preferably, a plurality of extending members are uniformly arranged on the stirring pipe, the extending members are U-shaped hollow flat pipes, and the interiors of the extending members are communicated with the stirring pipe.
Preferably, the heating rods are arranged in a ring shape, and the number of the heating rods is 8-15.
Preferably, a temperature sensor is arranged on the inner side wall of the tank body.
Preferably, the adjusting mechanism comprises two vertical plates fixed on the bottom surface of the tank body, a bottom plate is arranged between the two vertical plates, the bottom plate is an arc-shaped plate, and a rack is arranged on the bottom surface of the bottom plate.
Preferably, the adjusting mechanism further comprises a rotating rod rotatably mounted on the mounting frame, a second gear is arranged on the rotating rod and is in meshed connection with the rack, a second motor is mounted on the outer side wall of the mounting frame, and an output shaft of the second motor is connected with the rotating rod.
Compared with the prior art, the invention has the beneficial effects that:
1. when materials are added into the reaction kettle, the first motor is started to drive the inner shaft to rotate, so that the stirring pipe rotates along with the inner shaft, the first gear at the end part of the stirring pipe is meshed with the annular rack, the stirring pipe rotates, a plurality of extending pieces are uniformly arranged on the stirring pipe, the extending pieces rotate to stir the materials, the materials are mixed more fully, and therefore stirring efficiency is improved;
2. the heating rods are uniformly arranged in the tank body, extend into the material, the efficiency of the heating and stirring mechanism is higher, when the temperature is reduced, the cooling liquid is injected into the convex ring from the liquid injection pipe, then the cooling liquid enters the stirring pipe and the extending part, the effective cooling contact surface of the material is larger, the cooling efficiency is greatly improved, and the production efficiency is further improved;
3. after the reaction is finished, the electromagnetic valve on the discharge port is opened to discharge materials, and the second motor is started to drive the rotating rod and the second gear to rotate due to the adjusting mechanism which is convenient for dumping the materials, and the second gear is meshed with the rack to be connected, so that the angle of the tank body can be adjusted, the tank body is inclined by a certain angle, a large amount of materials can be prevented from remaining on the bottom surface of the tank body when the materials are dumped, and the materials are prevented from being wasted.
Drawings
FIG. 1 is a schematic view of the appearance of the device of the present invention;
FIG. 2 is a diagram of a pipe structure of the present invention;
FIG. 3 is a schematic view of the structure of the device of the present invention;
fig. 4 is an enlarged view at a in fig. 3.
In the figure: the device comprises a mounting frame 1, a tank body 2, a charging port 201, a mounting column 202, a discharging port 203, a first motor 3, an inner shaft 4, a stirring pipe 5, an extension piece 6, a convex ring 7, an inner rotary ring 8, a first gear 9, an annular rack 10, a liquid injection pipe 11, a liquid discharge pipe 12, a heating rod 13, a temperature sensor 14, a vertical plate 15, a bottom plate 16, a rack 17, a rotary rod 18, a second motor 19 and a second gear 20.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 4, the present invention provides a technical solution: an enzymatic synthesis of D-tryptophan, the synthesis comprising the steps of:
s1: placing L-tryptophan, a solvent and a racemization agent into a reaction kettle device, heating and stirring, adding a resolving agent, heating to 95 ℃, and carrying out heat preservation reaction;
s2: carrying out gradient cooling on the material obtained in the step S1, wherein each 5 ℃ is a gradient, and carrying out heat preservation reaction for a certain time at the temperature of the corresponding gradient;
s3: centrifuging the material obtained in the step S2 to obtain a mixture of D-tryptophan as a solid and a complex of a resolving agent, wherein a liquid phase is a mother solution of DL-tryptophan, D-tryptophan is obtained by decomplexing the solid and the resolving agent is recovered, and the mother solution of DL-tryptophan is recovered by adjusting pH=2-3 with HCl solution;
s4: and (3) washing the D-tryptophan obtained in the step (S3) with methanol once, performing centrifugal operation after washing, and drying the D-tryptophan obtained after centrifugal operation.
The reaction kettle device comprises a mounting frame 1, wherein the mounting frame 1 is of a structure, a tank body 2 is rotatably mounted on the mounting frame 1, a charging port 201 is arranged at the top of the tank body 2, a sealing cover is arranged on the charging port 201, one end of the sealing cover is hinged, the other end of the sealing cover is connected through a hasp, a mounting column 202 is arranged on the middle side wall of the tank body 2, the mounting column 202 penetrates through two side walls of the mounting frame 1, the mounting column 202 is rotatably connected with the mounting frame 1, a discharging port 203 is arranged on the bottom side wall of the tank body 2, and an electromagnetic valve is arranged on the discharging port 203.
The bottom surface of the tank body 2 is provided with a first motor 3, the output end of the first motor 3 is connected with an inner shaft 4, the inner shaft 4 penetrates through the bottom surface of the tank body 2, the inner shaft 4 is provided with a multifunctional stirring mechanism, the stirring mechanism comprises two stirring pipes 5, the stirring pipes 5 penetrate through the inner shaft 4, the stirring pipes 5 are rotationally connected with the inner shaft 4, two ends of the stirring pipes 5 penetrate through an inner rotating ring 8 and extend into a convex ring 7, the stirring pipes 5 are rotationally connected with the inner rotating ring 8, the stirring pipes 5 and the inner rotating ring 8 can be connected through a sealing bearing, one end of the stirring pipes 5 is provided with a first gear 9, the first gear 9 is meshed with an annular rack 10, a plurality of extending pieces 6 are uniformly arranged on the stirring pipes 5, the extending pieces 6 are U-shaped hollow flat pipes, and the interiors of the extending pieces 6 are communicated with the stirring pipes 5; after the material is added into the reaction kettle, the first motor 3 starts to drive the inner shaft 4 to rotate, so that the stirring pipe 5 rotates along with the inner shaft, the first gear 9 at the end part of the stirring pipe 5 is meshed with the annular rack 10 to be connected, the stirring pipe 5 rotates, a plurality of extending pieces 6 are uniformly arranged on the stirring pipe 5, the extending pieces 6 rotate to stir the material, the material is mixed more fully, and accordingly stirring efficiency is improved.
Be provided with two rings bulge loop 7 on the lateral wall of jar body 2, bulge loop 7's inside is provided with annular rack 10, bulge loop 7 internal rotation is installed, the internal rotation ring 8 is embedded in bulge loop 7, can be provided with the sealing ring between internal rotation ring 8 and the bulge loop 7 junction, prevent that the material from leaking, be connected with notes liquid pipe 11 and fluid-discharge tube 12 on the both sides outer wall of bulge loop 7, install heating rod 13 on the middle part inside wall of jar body 2, heating rod 13 is the annular setting, the number of piles that heating rod 13 was provided with is according to equipment size, the quantity of every layer heating rod 13 is 8-15, heating rod 13 extends into in the material, many heating rods 13 heat jointly, can improve heating efficiency greatly, install temperature sensor 14 on the inside wall of jar body 2, temperature sensor 14 is used for the temperature of real-time supervision inside material. When cooling, the coolant liquid is poured into the inside of bulge loop 7 from annotating liquid pipe 11, then the coolant liquid enters into inside stirring pipe 5, the extension piece 6, and the effective cooling contact surface of material is bigger, has improved cooling efficiency greatly to production efficiency has been improved.
The lower part of the tank body 2 is provided with an adjusting mechanism which is convenient for dumping materials, the adjusting mechanism comprises two vertical plates 15 fixed on the bottom surface of the tank body 2, a bottom plate 16 is arranged between the two vertical plates 15, the bottom plate 16 is an arc plate, the bottom surface of the bottom plate 16 is provided with a rack 17, the adjusting mechanism also comprises a rotating rod 18 rotatably arranged on the mounting frame 1, the rotating rod 18 is provided with a second gear 20, the second gear 20 is in meshed connection with the rack 17, the outer side wall of the mounting frame 1 is provided with a second motor 19, and the output shaft of the second motor 19 is connected with the rotating rod 18; after the reaction is finished, the electromagnetic valve on the discharge hole 203 is opened to discharge materials, and the second motor 19 is started to drive the rotating rod 18 and the second gear 20 to rotate due to the arrangement of the adjusting mechanism which is convenient for dumping the materials, the second gear 20 is meshed with the rack 17 to be connected, and the angle of the tank body 2 can be adjusted, so that the tank body 2 is inclined by a certain angle, a large amount of materials can be prevented from remaining on the bottom surface of the tank body 2 during dumping, and the materials are prevented from being wasted.
When the equipment is used, after materials are added into the reaction kettle, the first motor 3 is started to drive the inner shaft 4 to rotate, so that the stirring pipe 5 rotates along with the rotation, the first gear 9 at the end part of the stirring pipe 5 is meshed with the annular rack 10, so that the stirring pipe 5 rotates, the stirring pipe 5 is uniformly provided with a plurality of extending pieces 6, the materials are stirred by the rotating extending pieces 6, the materials are mixed more fully, and the stirring efficiency is improved; the plurality of heating rods 13 are uniformly arranged in the tank body 2, the heating rods 13 extend into the material, the efficiency of the heating and stirring mechanism is higher, when the temperature is reduced, the cooling liquid is injected into the convex ring 7 from the liquid injection pipe 11, then the cooling liquid enters the stirring pipe 5 and the extending part 6, the effective cooling contact surface of the material is larger, the cooling efficiency is greatly improved, and the production efficiency is further improved; after the reaction is finished, the electromagnetic valve on the discharge hole 203 is opened to discharge materials, and the second motor 19 is started to drive the rotating rod 18 and the second gear 20 to rotate due to the arrangement of the adjusting mechanism which is convenient for dumping the materials, the second gear 20 is meshed with the rack 17 to be connected, and the angle of the tank body 2 can be adjusted, so that the tank body 2 is inclined by a certain angle, a large amount of materials can be prevented from remaining on the bottom surface of the tank body 2 during dumping, and the materials are prevented from being wasted.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. An enzymatic synthesis method of D tryptophan, which is characterized by comprising the following steps: the equipment used in the synthesis method comprises a reaction kettle device, wherein the reaction kettle device comprises a mounting frame (1), the mounting frame (1) is of a type structure, a tank body (2) is rotatably mounted on the mounting frame (1), a charging port (201) is arranged at the top of the tank body (2), mounting columns (202) are arranged on the middle side wall of the tank body (2), the mounting columns (202) penetrate through the two side walls of the mounting frame (1), the mounting columns (202) are rotatably connected with the mounting frame (1), a discharge port (203) is arranged on the side wall of the bottom end of the tank body (2), and an electromagnetic valve is arranged on the discharge port (203);
the novel stirring device is characterized in that a first motor (3) is arranged on the bottom surface of the tank body (2), an output end of the first motor (3) is connected with an inner shaft (4), the inner shaft (4) penetrates through the bottom surface of the tank body (2), a multifunctional stirring mechanism is arranged on the inner shaft (4) and comprises two stirring pipes (5), a plurality of extending pieces (6) are uniformly arranged on the stirring pipes (5), the extending pieces (6) are U-shaped hollow flat pipes, the inside of the extending pieces (6) is communicated with the stirring pipes (5), the stirring pipes (5) penetrate through the inner shaft (4), the stirring pipes (5) are connected with the inner shaft (4) in a rotating mode, two ends of the stirring pipes (5) penetrate through an inner rotating ring (8) and extend into a convex ring (7), the stirring pipes (5) are connected with the inner rotating ring (8), a first gear (9) is arranged at one end of each stirring pipe (5), and the first gear (9) is connected with an annular rack (10) in a meshed mode.
Be provided with bulge loop (7) on the lateral wall of jar body (2), the inside of bulge loop (7) is provided with annular rack (10), inner swivel (8) are installed in bulge loop (7) internal rotation, be connected with notes liquid pipe (11) and fluid-discharge tube (12) on the both sides outer wall of bulge loop (7), install heating rod (13) on the middle part inside wall of jar body (2), the below of jar body (2) is provided with the guiding mechanism of being convenient for empty the material, guiding mechanism is including fixing two risers (15) in jar body (2) bottom surface, installs bottom plate (16) between two risers (15), and bottom plate (16) are the arc, are provided with rack (17) on the bottom surface of bottom plate (16), guiding mechanism is still including rotating dwang (18) of installing on mounting bracket (1), is provided with second gear (20) on dwang (18), and second gear (20) and rack (17) meshing are connected, and motor outside wall of mounting bracket (1) installs second output shaft (19) and is connected with second output shaft (19).
2. The method for enzymatic synthesis of D tryptophan according to claim 1, wherein: the heating rods (13) are arranged in a ring shape, and the number of the heating rods (13) is 8-15.
3. The method for enzymatic synthesis of D tryptophan according to claim 1, wherein: and a temperature sensor (14) is arranged on the inner side wall of the tank body (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210641477.2A CN114807260B (en) | 2022-06-08 | 2022-06-08 | Enzymatic synthesis mode of D-tryptophan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210641477.2A CN114807260B (en) | 2022-06-08 | 2022-06-08 | Enzymatic synthesis mode of D-tryptophan |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114807260A CN114807260A (en) | 2022-07-29 |
| CN114807260B true CN114807260B (en) | 2024-02-09 |
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Family Applications (1)
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| CN202210641477.2A Active CN114807260B (en) | 2022-06-08 | 2022-06-08 | Enzymatic synthesis mode of D-tryptophan |
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| JP2008061642A (en) * | 2006-08-10 | 2008-03-21 | Toyobo Co Ltd | Method for producing d-amino acid |
| CN103342676A (en) * | 2013-07-24 | 2013-10-09 | 马鞍山德鸿生物技术有限公司 | Method for synthetizing D-tryptophan |
| CN108103137A (en) * | 2017-09-15 | 2018-06-01 | 南通紫琅生物医药科技有限公司 | A kind of new process of chemical method and Production by Enzymes D-trp |
| CN208308870U (en) * | 2018-04-16 | 2019-01-01 | 长兴制药股份有限公司 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
| CN215996632U (en) * | 2021-07-19 | 2022-03-11 | 连云港康乐药业有限公司 | Acylation reaction tank with heating and cooling functions |
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2022
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008061642A (en) * | 2006-08-10 | 2008-03-21 | Toyobo Co Ltd | Method for producing d-amino acid |
| CN103342676A (en) * | 2013-07-24 | 2013-10-09 | 马鞍山德鸿生物技术有限公司 | Method for synthetizing D-tryptophan |
| CN108103137A (en) * | 2017-09-15 | 2018-06-01 | 南通紫琅生物医药科技有限公司 | A kind of new process of chemical method and Production by Enzymes D-trp |
| CN208308870U (en) * | 2018-04-16 | 2019-01-01 | 长兴制药股份有限公司 | A kind of Enzyme catalyzed synthesis D-Ser and DL-serine joint production process system |
| CN215996632U (en) * | 2021-07-19 | 2022-03-11 | 连云港康乐药业有限公司 | Acylation reaction tank with heating and cooling functions |
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