CN208224158U - A kind of direct bioelectrode analytical equipment for fermentation liquid detection - Google Patents
A kind of direct bioelectrode analytical equipment for fermentation liquid detection Download PDFInfo
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- CN208224158U CN208224158U CN201820750215.9U CN201820750215U CN208224158U CN 208224158 U CN208224158 U CN 208224158U CN 201820750215 U CN201820750215 U CN 201820750215U CN 208224158 U CN208224158 U CN 208224158U
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Abstract
A kind of direct bioelectrode analytical equipment and analysis method for fermentation liquid detection, comprising: reaction tank, bioelectrode, buffer kit and drain pump.After bioelectrode touches bio-sensing electrode after reaction a period of time, direct electrode transfer can be generated, form faint bioelectrochemistry kinetic current signal, when the concentration of substrate to be measured entered in reaction tank is high, the kinetic current of formation is also high, and it is linearly proportional within concentration range on one point.Due to the working chamber used in reaction tank the reaction cavity big for capacity, dilute fermentation broth sample concentration, provide the anti-interference ability for changing system, Quality Control, calibration, cleaning etc. are increased simultaneously surveys idle loop section, direct bioelectrode uses high-precision industrial grade Acquisition System for Weak Signal, can be with the Electro Magnetic Compatibility of collecting test system.
Description
Technical field
The present invention relates to biosensor technology fields, and in particular to a kind of direct bioelectrode for fermentation liquid detection
Analytical equipment.
Background technique
Biosensor (biosensor) be made using biological sensitive materials recognition component and physical and chemical energy converter appropriate and
The analysis tool or detection system that signal amplifying apparatus is constituted.Its core component is bioelectrode, including enzyme electrode, immune electricity
Pole, nucleic acid electrode, cell electrode, tissue electrode etc..It can be widely applied to medicine detection, residential care, food safety, industrial mistake
Journey, agriculture application, environment pollution control, military biochemical protection etc..
There are three developing stage for biosensor.By taking electrochemical enzyme electrode as an example, Clark and Lyons is mentioned for the first time within 1962
Enzyme electrode out belongs to first generation biosensor, also referred to as classical enzyme electrode.Its principle is transmitted using the native electron of enzyme
Body ----oxygen links up the electron channel between electrode, the direct reduction of detection enzyme reaction substrate or the generation of product.Second
It is mediator enzyme sensor (mediator enzyme electrode) for biosensor, is to replace oxygen molecule in enzyme using redox electron mediator
Redox active centre and electrode between transmit electronics, solve the dependence to oxygen and the interference problem of electrode activity.The
Three generations's biosensor is also referred to as Direct Electrochemistry enzyme electrode, is that the redox active centre of enzyme and electrode surface directly exchange
The enzyme sensor of electronics.
Direct bioelectrode mostly uses strip to print electrode, also referred to as reagent strip.It is special with being constantly progressive for manufacturing technology
Be not the development and application of nanotechnology, electrode performance be continuously improved, advantageous feature be high sensitivity, strong interference immunity,
Industrialized mass production can be achieved, it is at low cost, it is easy to use etc., thus have been more and more widely used.
Industrial fermentation processes need to carry out fast and accurately fermentation substrate, important mesostate and target product
Detection, to optimize and to control to fermentation process.The characteristics of fermentation broth sample is that sample size is big, batch is more, sample component
Complexity, measured object concentration variation range are wide, and environmental disturbances factor is unstable etc..It is usually that will be tested when reagent strip formula electrode use
Sample is added in electrode surface and is measured, belong to disposable detection method, accuracy and homogenieity by reagent strip manufacturing process,
The influence of storage and transportation mode and storage time is not able to satisfy the requirement of fermented sample high-precision detection.To improve the inspection of reagent strip electrode
The accuracy of survey, many new analytical technologies and analysis product start to apply, and have offshore company to develop Quality Control, calibration, clearly
The analyzer of functions such as wash.But these enzyme electrode analyzers all lay particular emphasis on the measurement of blood sample micro rank, and operation precision is wanted
It asks higher, is not suitable for the measurement under industry complex environment to fermentation broth sample.
Summary of the invention
Bioelectrode is connected using reaction tank to overcome the above deficiencies, the invention provides a kind of, realizes matter
Control, calibration, cleaning and one, the strong direct bioelectrode analytical equipment for fermentation liquid detection of anti-electromagnetic interference capability.
The present invention overcomes the technical solution used by its technical problem to be:
A kind of direct bioelectrode analytical equipment for fermentation liquid detection, comprising:
Reaction tank is internally provided with working chamber, and the leakage fluid dram and inlet being connected with working chamber are provided on reaction tank；
Bioelectrode is fixed on reaction tank, in the induction zone and working chamber of the bioelectrode by fixed device
Buffer is in contact, and each signal output end of the bioelectrode is connected to bioelectrode sensing analysis system；
Buffer kit, the liquid feeding end of cleaning pump is connected to by liquid suction pipe II, and outlet end is connected by inlet tube
It is connected to the inlet of reaction tank；And
Drain pump, liquid feeding end are connected to the leakage fluid dram of reaction tank by liquid suction pipe I, and outlet end is connected by drain pipe
In waste liquid box.
Above-mentioned fixed device includes the screw hole being set in reaction tank, and the tail end of the screw hole is connected with working chamber, raw
Object electrode is located in screw hole and the nut by screwing in screw hole is fixed.
It further include being set on reaction tank and being connected with working chamber to realize stirring evenly for solution on working chamber
Mounting hole II, agitating device are installed in mounting hole II, and agitating device stirs the buffer in working chamber.
It further include the mounting hole I for being set on reaction tank and being connected with working chamber, sample introduction to detect after realizing feed liquor
Trigger sensor is installed in mounting hole I, and trigger sensor is connected to bioelectrode sensing analysis system, when being filled in working chamber
When buffer, trigger sensor generates trigger signal.
It further include backing plate and the corresponding copper of several and bioelectrode being set on reaction tank to improve leakproofness
Line, the bioelectrode are in contact with backing plate, and circular hole is provided on backing plate, and sealing ring, sealing ring one are provided in the circular hole
End and the induction zone close contact of bioelectrode, the other end and working chamber close contact, on the backing plate with bioelectrode
The corresponding jack of each signal output end, each copper wire are in contact after passing through corresponding jack with corresponding signal output end.
Crushing bioelectrode in order to prevent further includes the pressing plate being set between bioelectrode and nut.
Further, above-mentioned trigger sensor is infrared light-emitting diode and infrared receiving diode, when not having in working chamber
When having buffer, infrared receiving diode receives the infrared light that infrared light-emitting diode issues, and generates electric signal.
The beneficial effects of the present invention are: can be produced after bioelectrode touches bio-sensing electrode after reaction a period of time
Raw direct electrode transfer, forms faint bioelectrochemistry kinetic current signal, when the concentration of substrate to be measured entered in reaction tank
When high, the kinetic current of formation is also high, and within concentration range on one point, linearly proportional.Directly biology
For electrode in biological process industry field, development and application are difficult, are primarily due to that fermentation broth sample amount is big, batch is more, sample
Caused by the factors such as complicated components, measured object concentration variation range are wide, and environmental disturbances factor is unstable.Due to being used in reaction tank
Working chamber be the big reaction cavity of capacity, dilute fermentation broth sample concentration, provide the anti-interference ability for changing system, simultaneously
It increasing Quality Control, calibration, cleaning etc. and surveys idle loop section, direct bioelectrode uses high-precision industrial grade Acquisition System for Weak Signal,
It can make the invention can be with Successful utilization to industrial environment with the Electro Magnetic Compatibility of collecting test system.
Detailed description of the invention
Fig. 1 is system construction drawing of the invention；
Fig. 2 is the three-dimensional structure diagram I of reaction tank of the invention；
Fig. 3 is the three-dimensional structure diagram II of reaction tank of the invention；
In figure, the emptying of 1. reaction tank, 2. agitating device, 3. sample introduction trigger sensor, 4. bioelectrode, 5. working chamber 6.
Pump 7. liquid suction pipe, I 8. drain pipe, 9. waste liquid box, 10. buffer kit, 11. liquid suction pipe, II 12. cleaning pump, 13. feed liquor
20. pressing plate of pipe 14. mounting hole, I 15. mounting hole, II 16. leakage fluid dram, 17. inlet, 18. copper wire, 19. nut, 21. backing plate
22. 23. jack of sealing ring.
Specific embodiment
With reference to the accompanying drawing 1, attached drawing 2, the present invention will be further described for attached drawing 3.
A kind of direct bioelectrode analytical equipment for fermentation liquid detection, comprising: reaction tank 1 is internally provided with work
Make chamber 5, the leakage fluid dram 16 and inlet 17 being connected with working chamber 5 are provided on reaction tank 1；Bioelectrode 4 passes through fixed device
It is fixed on reaction tank 1, the induction zone of the bioelectrode 4 is in contact with the buffer in working chamber 5, the bioelectrode 4
Each signal output end be connected to bioelectrode sensing analysis system；Buffer kit 10 is connected by liquid suction pipe II 11
It is connected to the liquid feeding end of cleaning pump 12, outlet end is connected to the inlet 17 of reaction tank 1 by inlet tube 13；And drain pump 6,
Its liquid feeding end is connected to the leakage fluid dram 16 of reaction tank 1 by liquid suction pipe I 7, and outlet end is connected to waste liquid box by drain pipe 8
9.The buffer in buffer kit 10 is sent into the working chamber 5 in reaction tank 1 by the work of cleaning pump 12 when work, Zhi Hou
Make its reaction after sample is added in working chamber 5, after bioelectrode 4 touches bio-sensing electrode after reaction a period of time, meeting
Direct electrode transfer is generated, faint bioelectrochemistry kinetic current signal is formed, when the substrate to be measured entered in reaction tank 1 is dense
When spending high, the kinetic current of formation is also high, and within concentration range on one point, linearly proportional.It is directly raw
For object electrode in biological process industry field, development and application are difficult, are primarily due to that fermentation broth sample amount is big, batch is more, sample
Caused by the factors such as product complicated components, measured object concentration variation range are wide, and environmental disturbances factor is unstable.Due in reaction tank 1
The working chamber 5 used the reaction cavity big for capacity, dilutes fermentation broth sample concentration, provides the anti-interference energy for changing system
Power, while increasing Quality Control, calibration, cleaning etc. and surveying idle loop section, direct bioelectrode is adopted using high-precision industrial grade small-signal
Collecting system can make the invention can be with Successful utilization to industrial environment with the Electro Magnetic Compatibility of collecting test system.After reaction
Solution can be drained pump and 6 extracted out from working chamber 5, and be sent into waste liquid box 9, test next time facilitated to use.
Preferably, fixed device includes the screw hole being set in reaction tank 1, and the tail end of the screw hole is connected with working chamber
Logical, bioelectrode 4 is located in screw hole and the nut 19 by screwing in screw hole is fixed.Its mounting structure is simple, operation side
Just.
It can also include the mounting hole II 15 for being set on reaction tank 1 and being connected with working chamber 5, agitating device 2 is installed
In mounting hole II 15, agitating device 2 stirs the buffer in working chamber 5.It can will be in working chamber 5 by agitating device 2
Solution stir evenly, improve measurement when accuracy.
It can also include the mounting hole I 14 for being set on reaction tank 1 and being connected with working chamber 5, sample introduction trigger sensor
3 are installed in mounting hole I 14, and trigger sensor 3 is connected to bioelectrode sensing analysis system, when being filled with buffering in working chamber 5
When liquid, trigger sensor 3 generates trigger signal.Trigger signal is generated by trigger sensor 3, can control bioelectrode biography
Sense analysis system starts timing, to facilitate the control reaction time.
It further, further include backing plate 21 and the corresponding copper wire of several and bioelectrode 4 being set on reaction tank 1
18, the bioelectrode 4 is in contact with backing plate 21, and circular hole is provided on backing plate 21, and sealing ring 22 is provided in the circular hole, close
The induction zone close contact of 22 one end of seal and bioelectrode 4, the other end and 5 close contact of working chamber, on the backing plate 21
Jack 23 corresponding with each signal output end of bioelectrode 4, each copper wire 18 pass through corresponding jack 23 after with it is corresponding
Signal output end be in contact.By increasing backing plate 21 and installing sealing ring 22 on backing plate 21, so as to improve sealing
Property, so that sample solution to the induction zone with bioelectrode 4 in working chamber 5 is contacted with each other.Each signal of bioelectrode 4 exports
End with copper wire 18 by being connected, so as to be convenient for transmitting signals to bioelectrode sensing analysis system using copper wire 18.
It preferably, further include the pressing plate 20 being set between bioelectrode 4 and nut 19.By in nut 19 and bioelectricity
Pressing plate 20 is set between pole 4, bioelectrode 4 can be prevented by crushing, improve the reliability used.
Specifically, trigger sensor 3 can be infrared light-emitting diode and infrared receiving diode, do not have when in working chamber 5
When having buffer, infrared receiving diode receives the infrared light that infrared light-emitting diode issues, and generates electric signal.Work as working chamber
After 5 are passed through buffer, the infrared light that infrared light-emitting diode issues is stopped by buffer, and infrared receiving diode can not receive,
So as to generate shutdown, make change in electric, bioelectrode sensing analysis system in working chamber 5 it can be learnt that be passed through slow at this time
Fliud flushing facilitates it to start timing, so that operator be helped to learn the reaction time.
Claims (7)
1. a kind of direct bioelectrode analytical equipment for fermentation liquid detection characterized by comprising
Reaction tank (1) is internally provided with working chamber (5), and the leakage fluid dram being connected with working chamber (5) is provided on reaction tank (1)
(16) and inlet (17)；
Bioelectrode (4) is fixed on reaction tank (1), the induction zone and working chamber of the bioelectrode (4) by fixed device
(5) buffer in is in contact, and each signal output end of the bioelectrode (4) is connected to bioelectrode sensing analysis system
System；
Buffer kit (10), the liquid feeding end of cleaning pump (12) is connected to by liquid suction pipe II (11), and outlet end passes through
Inlet tube (13) is connected to the inlet (17) of reaction tank (1)；And
Drain pump (6), liquid feeding end are connected to the leakage fluid dram (16) of reaction tank (1) by liquid suction pipe I (7), and outlet end passes through
Drain pipe (8) is connected to waste liquid box (9).
2. the direct bioelectrode analytical equipment according to claim 1 for fermentation liquid detection, it is characterised in that: described
Fixed device includes the screw hole being set in reaction tank (1), and the tail end of the screw hole is connected with working chamber, bioelectrode (4)
In screw hole and the nut (19) by screwing in screw hole is fixed.
3. the direct bioelectrode analytical equipment according to claim 1 for fermentation liquid detection, it is characterised in that: also wrap
The mounting hole II (15) for being set on reaction tank (1) and being connected with working chamber (5) is included, agitating device (2) is installed on mounting hole
In II (15), agitating device (2) stirs the buffer in working chamber (5).
4. the direct bioelectrode analytical equipment according to claim 1 for fermentation liquid detection, it is characterised in that: also wrap
The mounting hole I (14) for being set on reaction tank (1) and being connected with working chamber (5) is included, sample introduction trigger sensor (3) is installed on peace
It fills in hole I (14), trigger sensor (3) is connected to bioelectrode sensing analysis system, is filled with buffer when working chamber (5) is interior
When, trigger sensor (3) generates trigger signal.
5. the direct bioelectrode analytical equipment according to claim 2 for fermentation liquid detection, it is characterised in that: also wrap
Several copper wire (18) corresponding with bioelectrode (4) for including backing plate (21) and being set on reaction tank (1), the bioelectricity
Pole (4) is in contact with backing plate (21), and circular hole is provided on backing plate (21), and sealing ring (22), sealing ring are provided in the circular hole
(22) the induction zone close contact of one end and bioelectrode (4), the other end and working chamber (5) close contact, the backing plate
(21) jack (23) corresponding with each signal output end of bioelectrode (4) on, each copper wire (18) pass through corresponding insert
Hole (23) is in contact with corresponding signal output end afterwards.
6. the direct bioelectrode analytical equipment according to claim 2 for fermentation liquid detection, it is characterised in that: also wrap
Include the pressing plate (20) being set between bioelectrode (4) and nut (19).
7. the direct bioelectrode analytical equipment according to claim 4 for fermentation liquid detection, it is characterised in that: described
Trigger sensor (3) is infrared light-emitting diode and infrared receiving diode, infrared when not having buffer in the working chamber (5)
Reception diode receives the infrared light that infrared light-emitting diode issues, and generates electric signal.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820750215.9U CN208224158U (en) | 2018-05-21 | 2018-05-21 | A kind of direct bioelectrode analytical equipment for fermentation liquid detection |
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| CN201820750215.9U CN208224158U (en) | 2018-05-21 | 2018-05-21 | A kind of direct bioelectrode analytical equipment for fermentation liquid detection |
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| CN208224158U true CN208224158U (en) | 2018-12-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426938A (en) * | 2018-05-21 | 2018-08-21 | 山东省科学院生物研究所 | A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection |
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2018
- 2018-05-21 CN CN201820750215.9U patent/CN208224158U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426938A (en) * | 2018-05-21 | 2018-08-21 | 山东省科学院生物研究所 | A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection |
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