CN214310287U - Double-index enzyme electrode detection device - Google Patents
Double-index enzyme electrode detection device Download PDFInfo
- Publication number
- CN214310287U CN214310287U CN202023053891.7U CN202023053891U CN214310287U CN 214310287 U CN214310287 U CN 214310287U CN 202023053891 U CN202023053891 U CN 202023053891U CN 214310287 U CN214310287 U CN 214310287U
- Authority
- CN
- China
- Prior art keywords
- enzyme electrode
- reaction tank
- liquid bottle
- pipeline
- pump valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model relates to a biosensor technical field especially relates to a two index enzyme electrode detection device. The utility model provides a double-index enzyme electrode detection device, which comprises a reaction tank, a substrate enzyme electrode, a product enzyme electrode, a buffer liquid bottle, a standard liquid bottle and a sample liquid bottle, wherein the reaction tank comprises a first reaction tank and a second reaction tank which are connected in series through a pipeline; a substrate enzyme electrode is arranged in the first reaction tank, and a product enzyme electrode is arranged in the second reaction tank; and the buffer liquid bottle, the standard liquid bottle and the sample liquid bottle are gathered through pipelines and then are respectively connected with the first reaction tank and the second reaction tank through pipelines. The double-index enzyme electrode detection device can realize simultaneous detection of low-concentration and high-concentration substances without additional dilution outside an instrument, and improves the detection accuracy.
Description
Technical Field
The utility model relates to a biosensor technical field especially relates to a two index enzyme electrode detection device.
Background
The biosensor is an analysis tool or detection system composed of a biological sensitive material as an identification element, a proper physicochemical transducer and a signal amplification device. The immobilized enzyme sensor (enzyme electrode) is a biosensor which is most researched, stable in performance and largest in application market, has the advantages of good specificity, simple and quick sample pretreatment, high sensitivity and strong operability, and has wide application prospect in the fields of microbial fermentation and cell culture process detection and control.
The biological reaction (cell culture, microbial fermentation, etc.) process requires rapid and accurate detection of substrates and target products in order to optimize and control the biological reaction process. Taking glutamic acid fermentation as an example, the conventional online detection device adopting enzyme electrode dual indexes (substrate glucose and product glutamic acid) is that a glucose electrode and a glutaminase electrode are arranged in the same reaction pool cavity (as shown in figure 2). Meanwhile, the fermentation liquor detection sample is characterized by large sample amount, multiple batches, complex sample components, wide concentration variation range of the detected object and the like. In actual measurement, the concentration of the analyte is too high, and thus the analyte needs to be diluted for measurement (glutamic acid and glucose are diluted at the same time). And injecting the diluted sample into the reaction pool cavity, and obtaining the glucose and glutamic acid measurement results after 20 seconds. However, the concentration changes of the substrate and the target product in the fermentation process show opposite change trends: in the initial stage of fermentation, the concentration of the substrate is high, and the concentration of the product is low (almost zero). With the progress of the fermentation time, the substrate is gradually consumed, and the concentration is gradually reduced; the product is gradually generated, and the concentration is gradually increased. At the end of the fermentation, the product concentration reaches a maximum and the substrate approaches zero. Therefore, when the conventional detection shown in fig. 2 is used for actual detection by using an enzyme electrode dual-index (substrate glucose and product glutamic acid) online detection device, because the concentration of the detected substance is too high, a sample needs to be diluted to a certain extent before detection, and then enters the detection device for accurate detection. The concentrations of the product at the initial stage of fermentation and the substrate at the end of fermentation are within the detection range of the instrument and can be measured without dilution. In the actual test process, the glucose and glutamic acid are measured according to a unified operation procedure, and the dilution factor of the sample to be measured is determined according to the high-concentration detection range. This results in a low concentration sample that is unnecessarily diluted with an error. Especially at the end of the fermentation, substrate concentrations close to 0 (raw material utilization) are required. Due to dilution detection of the sample, dilution errors are caused, and accurate judgment of a substrate (residual quantity) and a fermentation end point is not facilitated.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a two index enzyme electrode detection device, two index enzyme electrode detection device need not carry out extra dilution outside the instrument, have improved the accuracy that detects.
In order to realize the purpose of the utility model, the utility model provides a following technical scheme:
the utility model provides a double-index enzyme electrode detection device, which comprises a reaction tank, a substrate enzyme electrode 3, a product enzyme electrode 4, a buffer liquid bottle 6, a standard liquid bottle 7 and a sample liquid bottle 8, wherein the reaction tank comprises a first reaction tank 1 and a second reaction tank 2 which are connected in series through a pipeline;
a substrate enzyme electrode 3 is arranged in the first reaction tank 1, and a product enzyme electrode 4 is arranged in the second reaction tank 2;
and the buffer liquid bottle 6, the standard liquid bottle 7 and the sample liquid bottle 8 are gathered through pipelines and then are respectively connected with the first reaction tank 1 and the second reaction tank 2 through pipelines.
Preferably, the double-index enzyme electrode detection device further comprises a waste liquid bottle 9.
Preferably, stirrers 5 are arranged in the first reaction tank 1 and the second reaction tank 2.
Preferably, a first pump valve 10 is arranged at the pipeline collecting position of the buffer liquid bottle 6 and the standard liquid bottle 7;
a second pump valve 11 is arranged at the pipeline collecting position of the standard liquid bottle 7 and the sample liquid bottle 8;
a third pump valve 12 is arranged on a pipeline between the second pump valve 11 and the second reaction tank 2;
a fourth pump valve 13 is arranged on a pipeline between the second pump valve 11 and the first reaction tank 2;
the first pump valve 10, the second pump valve 11, the third pump valve 12 and the fourth pump valve 13 are three-way pump valves.
Preferably, the double-index enzyme electrode detection device further comprises a pipeline a, a pipeline b, a pipeline c, a pipeline d and a pipeline e;
the pipeline a is positioned between the second pump valve 11 and the fourth pump valve 13;
the pipeline b is positioned between the second pump valve 11 and the third pump valve 12;
the pipeline c, the pipeline d and the pipeline e are connected through a three-way valve;
said conduit c is located between said fourth pump valve 13 and said three-way valve;
the conduit d is located between the third pump valve 12 and the three-way valve;
the conduit e is located between the three-way valve and the waste bottle 9.
The utility model provides a double-index enzyme electrode detection device, which comprises a reaction tank, a substrate enzyme electrode 3, a product enzyme electrode 4, a buffer liquid bottle 6, a standard liquid bottle 7 and a sample liquid bottle 8, wherein the reaction tank comprises a first reaction tank 1 and a second reaction tank 2 which are connected in series through a pipeline; a substrate enzyme electrode 3 is arranged in the first reaction tank 1, and a product enzyme electrode 4 is arranged in the second reaction tank 2; and the buffer liquid bottle 6, the standard liquid bottle 7 and the sample liquid bottle 8 are gathered through pipelines and then are respectively connected with the first reaction tank 1 and the second reaction tank 2 through pipelines. The utility model discloses install substrate enzyme electrode 3 and product enzyme electrode 4 respectively in two reaction tanks, two reaction tanks are linked together by the pipeline, form the tandem structure, when beginning to survey, await measuring appearance at first pours into the second reaction tank 2 that is provided with product enzyme electrode 4 into. And when the product concentration is increased and the substrate concentration is reduced to a certain concentration in the fermentation process, converting the detection sequence of the detected sample, namely injecting the detected sample into the first reaction tank provided with the substrate enzyme electrode 3. Therefore, the low-concentration sample can be directly measured in the linear range of the electrode response without being diluted in advance, so that the error caused by sample pretreatment (dilution) is avoided, and the detection accuracy is improved. And the sample of high concentration is through the dilution of two reaction tanks, dilute in the testing process promptly, increased the automatic "dilution" function of sample in detecting system, because the error that this dilution produced can be standardized through standard solution and eliminated, so, make final test result simpler, convenient while, still improved the accuracy that detects.
Drawings
FIG. 1 is a schematic diagram of the structure of the double-index enzyme electrode detection device of the present invention, wherein, 1-the first reaction tank, 2-the second reaction tank, 3-the substrate enzyme electrode, 4-the product enzyme electrode, 5-the stirrer, 6-the buffer liquid bottle, 7-the standard liquid bottle, 8-the sample liquid bottle, 9-the waste liquid bottle, 10-the first pump valve, 11-the second pump valve, 12-the third pump valve, 13-the fourth pump valve;
FIG. 2 is a schematic diagram of the structure of the existing device for on-line detection by using enzyme electrode double indicators, wherein, 14-reaction cell module, 15-reaction cell cavity, 16-o-shaped ring of enzyme membrane, 17-sample introduction and waste liquid outflow channel, 18-reaction cell top cap, 19-sample introduction cap, 20-sample introduction optical sensor, 21-enzyme electrode, 22-enzyme electrode knob, 23-buffer solution inlet and waste liquid evacuation tube, 24-waste liquid precipitation tube, 25-electromagnetic stirrer, and 26-electrode lead.
Detailed Description
The utility model provides a double-index enzyme electrode detection device, which comprises a reaction tank, a substrate enzyme electrode 3, a product enzyme electrode 4, a buffer liquid bottle 6, a standard liquid bottle 7 and a sample liquid bottle 8, wherein the reaction tank comprises a first reaction tank 1 and a second reaction tank 2 which are connected in series through a pipeline;
a substrate enzyme electrode 3 is arranged in the first reaction tank 1, and a product enzyme electrode 4 is arranged in the second reaction tank 2;
and the buffer liquid bottle 6, the standard liquid bottle 7 and the sample liquid bottle 8 are gathered through pipelines and then are respectively connected with the first reaction tank 1 and the second reaction tank 2 through pipelines.
In a specific embodiment of the present invention, the dual-index enzyme electrode detection device further comprises a waste liquid bottle 9.
In a specific embodiment of the present invention, stirrers 5 are disposed in the first reaction tank 1 and the second reaction tank 2. The utility model discloses in, stirring 5 can make whole testing process go on under the condition of stirring to the messenger waits to detect liquid when getting into first reaction tank or second reaction tank and the quick and abundant mixture of the buffer solution in the inside.
In a specific embodiment of the present invention, a first pump valve 10 is disposed at a pipeline junction of the buffer liquid bottle 6 and the standard liquid bottle 7;
a second pump valve 11 is arranged at the pipeline collecting position of the standard liquid bottle 7 and the sample liquid bottle 8;
a third pump valve 12 is arranged on a pipeline between the second pump valve 11 and the second reaction tank 2;
a fourth pump valve 13 is arranged on a pipeline between the second pump valve 11 and the first reaction tank 2;
the first pump valve 10, the second pump valve 11, the third pump valve 12 and the fourth pump valve 13 are three-way pump valves.
In a specific embodiment of the present invention, the dual-index enzyme electrode detection device further includes a pipeline a, a pipeline b, a pipeline c, a pipeline d, and a pipeline e;
the pipeline a is positioned between the second pump valve 11 and the fourth pump valve 13;
the pipeline b is positioned between the second pump valve 11 and the third pump valve 12;
the pipeline c, the pipeline d and the pipeline e are connected through a three-way valve;
said conduit c is located between said fourth pump valve 13 and said three-way valve;
the conduit d is located between the third pump valve 12 and the three-way valve;
the conduit e is located between the three-way valve and the waste bottle 9.
The utility model discloses in, above-mentioned pipeline a, pipeline b, pipeline c, pipeline d, pipeline e, first pump valve 10, second pump valve 11, third pump valve 12 and fourth pump valve 13 set up and can guarantee two index enzyme electrode detection device divide into two routes: wherein, the path 1 is a second pump valve 11, a pipeline b, a second reaction tank 2, a first reaction tank 1, a pipeline c, a pipeline e and a waste liquid bottle 9; the path 2 comprises a second pump valve 11, a pipeline a, a first reaction tank 1, a second reaction tank 2, a pipeline d, a pipeline e and a waste liquid bottle 9.
The utility model also provides an above-mentioned technical scheme enzyme electrode reaction tank realize the online test method of bioreactor substrate and result, including following step:
a reaction pool and a pipeline in the double-index enzyme electrode detection device are filled with buffer solution;
the standard solution passes through the second reaction tank 2 and the first reaction tank 1 in sequence, and the response signal values of the product enzyme electrode are recorded as B1And the value of the substrate electrode response signal is A1；
The standard solution passes through a first reaction tank 1 and a second reaction tank 2 in sequence, and the corresponding response signal values of the product enzyme electrodes are recorded as B2And the value of the substrate electrode response signal is A2；
At the initial stage of fermentation, the liquid to be detected sequentially passes through the second reaction tank 2 and the first reaction tank 1, and the response signal values A of the substrate enzyme electrode 3 are respectively recordedx1And the product enzyme electrode 4 responds with a signal value Bx1(ii) a The initial fermentation period is from the initial fermentation time to BxThe concentration range of (A) is less than or equal to 100 mg/dL;
the determination result of the product in the solution to be determined is as follows: b isx＝Bx1/B1×100mg/dL；
The determination result of the substrate in the solution to be determined is as follows: a. thex＝Ax1/A1×100mg/dL；
As the fermentation proceeds, when BxIn a concentration range of greater than 100mg/dL and AxWhen the concentration range is more than 100mg/dL, the solution to be detected firstly passes through the second reaction tank 2 and the first reaction tank 1 in sequence, and the response signal values of the substrate enzyme electrode 3 are recorded as Ax21And the product enzyme electrode 4 responds with a signal value Bx21(ii) a Then sequentially passes through a first reaction tank 1 and a second reaction tank 2, and the response signal values of the substrate enzyme electrode 3 are respectively recorded as Ax22And the product enzyme electrode 4 responds with a signal value Bx22；
The determination result of the product in the solution to be determined is as follows: b isx＝Bx22/B2×100mg/dL；
The determination result of the substrate in the solution to be determined is as follows: a. thex＝Ax21/A1×100mg/dL；
When B is presentxIn a concentration range of greater than 100mg/dL and AxWhen the concentration range of the substrate enzyme electrode is less than or equal to 100mg/dL, the solution to be detected sequentially passes through a first reaction tank 1 and a second reaction tank 2, and the response signal values of a substrate enzyme electrode 3 are respectively recorded as Ax3And the product enzyme electrode 4 responds with a signal value Bx3；
The determination result of the product in the solution to be determined is as follows: b isx＝Bx3/B2×100mg/dL；
The determination result of the substrate in the solution to be determined is as follows: a. thex＝Ax3/A2×100mg/dL；
Wherein, BxTo eliminate the response signal value of the product enzyme electrode in the solution to be tested after system error, AxEliminating the response signal value of a product enzyme electrode in the solution to be detected after system errors; x is the time point of the test.
The utility model discloses be in fill with the buffer solution in the two index enzyme electrode detection devices. Before testing, the utility model discloses preferably will double index enzyme electrode detection device replaces the double index enzyme electrode detection device among the SBA-40 biosensing analysis appearance. In the present invention, the buffer solution is preferably a phosphate buffer solution having a pH of 7.0 and a concentration of 0.2 mol/L. Preferably, the double-index enzyme electrode detection device is filled with a buffer solution, the buffer solution passes through the path 1 and the path 2 respectively, and the pipeline and the reaction pool are filled with the buffer solution. In the utility model, the standard solution is preferably 100mg/dL product-100 mg/dL substrate mixed standard solution.
The dual-index enzyme electrode detection device provided by the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The instrument comprises the following steps: SBA-40 biosensing analyzer, wherein the double-index enzyme electrode detection device is replaced by the double-index enzyme electrode detection device of the utility model, the structure of the double-index enzyme electrode detection device is shown in figure 1;
reagent: 1) standard solution: 100mg/dL glutamic acid-100 mg/dL glucose mixed standard solution;
2) known concentrations of the test solution: 5mg/dL glutamic acid-1000 mg/dL glucose mixed liquor;
3) buffering agent: 0.2M phosphate buffer, pH 7.0;
4) an electrode: substrate enzyme electrode: an immobilized glucose oxidase electrode (a buffer solution is dripped on an electrode head, and a glucose oxidase film is pressed on the surface of the electrode head);
product enzyme electrode: an immobilized glutamate oxidase electrode (a buffer solution is dripped on an electrode head, and a glutamate oxidase film is pressed on the surface of an electrode head);
and (3) detection process:
switching on a power supply;
turning on an instrument switch, automatically entering a cleaning program by the instrument, and respectively filling buffer solution in the double-index enzyme electrode detection device through a path 1 and a path 2;
calibration: the standard solution passes through the second reaction tank 2 and the first reaction tank 1 in sequence, and the response signal values of the product enzyme electrode are recorded as B18154 and a substrate electrode response signal value of A1＝516；
The standard solution passes through a first reaction tank 1 and a second reaction tank 2 in sequence, and the corresponding response signal values of the product enzyme electrodes are recorded as B2534 and a substrate electrode response signal value of a2＝8351；
And (3) sample determination:
the liquid to be detected sequentially passes through the second reaction tank 2 and the first reaction tank 1, and the response signal value of the product enzyme electrode is Bx1412 and substrate enzyme electrode 3 response signal value ax1＝5173；
The determination result of the product (glutamic acid) in the solution to be determined is as follows: b isx＝Bx1/B1×100＝412/8154×100＝5.05mg/dL；
The determination result of the substrate (glucose) in the solution to be determined is as follows: a. thex＝Ax1/A1×100＝5173/516×100＝1002.52mg/dL。
Example 2
The instrument comprises the following steps: SBA-40 biosensing analyzer, wherein the double-index enzyme electrode detection device is replaced by the double-index enzyme electrode detection device of the utility model, the structure of the double-index enzyme electrode detection device is shown in figure 1;
reagent: 1) standard solution: 100mg/dL glutamic acid-100 mg/dL glucose mixed standard solution;
2) known concentrations of the test solution: 150mg/dL glutamic acid-500 mg/dL glucose mixed solution;
3) buffering agent: 0.2M phosphate buffer, pH 7.0;
4) an electrode: substrate enzyme electrode: an immobilized glucose oxidase electrode (a buffer solution is dripped on an electrode head, and a glucose oxidase film is pressed on the surface of the electrode head);
product enzyme electrode: an immobilized glutamate oxidase electrode (a buffer solution is dripped on an electrode head, and a glutamate oxidase film is pressed on the surface of an electrode head);
and (3) detection process:
switching on a power supply;
turning on an instrument switch, automatically entering a cleaning program by the instrument, and respectively filling buffer solution in the double-index enzyme electrode detection device through a path 1 and a path 2;
calibration: the standard solution passes through the second reaction tank 2 and the first reaction tank 1 in sequence, and the response signal values of the product enzyme electrode are recorded as B18154 and a substrate electrode response signal value of A1＝516；
The standard solution passes through a first reaction tank 1 and a second reaction tank 2 in sequence, and the corresponding response signal values of the product enzyme electrodes are recorded as B2534 and a substrate electrode response signal value of a2＝8351；
And (3) sample determination:
the liquid to be detected sequentially passes through the second reaction tank 2 and the first reaction tank 1, and the response signal value of the product enzyme electrode is Bx2112254 and substrate enzyme electrode 3 response signal value Ax2125990; then sequentially passes through a first reaction tank 1 and a second reaction tank 2, and the response signal value of the product enzyme electrode is Bx22812 and a substrate enzyme electrode 3 response signal value of ax21＝12571；
The determination result of the product (glutamic acid) in the solution to be determined is as follows: b isx＝Bx22/B2×100＝812/534×100＝152.06mg/dL；
The determination result of the substrate (glucose) in the solution to be determined is as follows: a. thex＝Ax21/A2×100＝25990/516×100＝502.42mg/dL。
Example 3
The instrument comprises the following steps: SBA-40 biosensing analyzer, wherein the double-index enzyme electrode detection device is replaced by the double-index enzyme electrode detection device of the utility model, the structure of the double-index enzyme electrode detection device is shown in figure 1;
reagent: 1) standard solution: 100mg/dL glutamic acid-100 mg/dL glucose mixed standard solution;
2) known concentrations of the test solution: 200mg/dL glutamic acid-50 mg/dL glucose mixed solution;
3) buffering agent: 0.2M phosphate buffer, pH 7.0;
4) an electrode: substrate enzyme electrode: an immobilized glucose oxidase electrode (a buffer solution is dripped on an electrode head, and a glucose oxidase film is pressed on the surface of the electrode head);
product enzyme electrode: an immobilized glutamate oxidase electrode (a buffer solution is dripped on an electrode head, and a glutamate oxidase film is pressed on the surface of an electrode head);
and (3) detection process:
switching on a power supply;
turning on an instrument switch, automatically entering a cleaning program by the instrument, and respectively filling buffer solution in the double-index enzyme electrode detection device through a path 1 and a path 2;
calibration: the standard solution passes through the second reaction tank 2 and the first reaction tank 1 in sequence, and the response signal values of the product enzyme electrode are recorded as B18154 and a substrate electrode response signal value of A1＝516；
The standard solution passes through a first reaction tank 1 and a second reaction tank 2 in sequence, and the corresponding response signal values of the product enzyme electrodes are recorded as B2534 and a substrate electrode response signal value of a2＝8351；
And (3) sample determination:
the liquid to be detected passes through the first reaction tank 1 and the second reaction tank 2 in turn, and the response signal value of the product enzyme electrode is Bx31062 and substrate enzyme electrode 3 response signal value ax3＝4166；
The determination result of the product (glutamic acid) in the solution to be determined is as follows: b isx＝Bx3/B2×100＝1062/534×100＝198.88mg/dL；
The determination result of the substrate (glucose) in the solution to be determined is as follows: a. thex＝Ax3/A2×100＝4166/8351×100＝49.89mg/dL。
Therefore, the result accuracy of the double-index enzyme electrode detection device is higher.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A double-index enzyme electrode detection device comprises a reaction pool, a substrate enzyme electrode (3), a product enzyme electrode (4), a buffer liquid bottle (6), a standard liquid bottle (7) and a sample liquid bottle (8), and is characterized in that the reaction pool comprises a first reaction pool (1) and a second reaction pool (2) which are connected in series through a pipeline;
a substrate enzyme electrode (3) is arranged in the first reaction tank (1), and a product enzyme electrode (4) is arranged in the second reaction tank (2);
the buffer liquid bottle (6), the standard liquid bottle (7) and the sample liquid bottle (8) are gathered through pipelines and then are respectively connected with the first reaction tank (1) and the second reaction tank (2) through pipelines;
the double-index enzyme electrode detection device also comprises a waste liquid bottle (9).
2. The dual-index enzyme electrode detection device according to claim 1, wherein stirrers (5) are arranged in the first reaction cell (1) and the second reaction cell (2).
3. The dual-index enzyme electrode detection device as claimed in claim 1, wherein a first pump valve (10) is arranged at the pipeline junction of the buffer liquid bottle (6) and the standard liquid bottle (7);
a second pump valve (11) is arranged at the pipeline collecting position of the standard liquid bottle (7) and the sample liquid bottle (8);
a third pump valve (12) is arranged on a pipeline between the second pump valve (11) and the second reaction tank (2);
a fourth pump valve (13) is arranged on a pipeline between the second pump valve (11) and the first reaction tank (1);
the first pump valve (10), the second pump valve (11), the third pump valve (12) and the fourth pump valve (13) are three-way pump valves.
4. The dual-index enzyme electrode detection device according to claim 3, further comprising a conduit a, a conduit b, a conduit c, a conduit d, and a conduit e;
the pipeline a is positioned between the second pump valve (11) and the fourth pump valve (13);
the pipeline b is positioned between the second pump valve (11) and the third pump valve (12);
the pipeline c, the pipeline d and the pipeline e are connected through a three-way valve;
the conduit c is located between the fourth pump valve (13) and the three-way valve;
the conduit d is located between the third pump valve (12) and the three-way valve;
the pipeline e is positioned between the three-way valve and the waste liquid bottle (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023053891.7U CN214310287U (en) | 2020-12-17 | 2020-12-17 | Double-index enzyme electrode detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023053891.7U CN214310287U (en) | 2020-12-17 | 2020-12-17 | Double-index enzyme electrode detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214310287U true CN214310287U (en) | 2021-09-28 |
Family
ID=77859454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023053891.7U Active CN214310287U (en) | 2020-12-17 | 2020-12-17 | Double-index enzyme electrode detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214310287U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112505122A (en) * | 2020-12-17 | 2021-03-16 | 山东省科学院生物研究所 | Double-index enzyme electrode detection device and online test method for substrate and product in bioreactor |
-
2020
- 2020-12-17 CN CN202023053891.7U patent/CN214310287U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112505122A (en) * | 2020-12-17 | 2021-03-16 | 山东省科学院生物研究所 | Double-index enzyme electrode detection device and online test method for substrate and product in bioreactor |
| CN112505122B (en) * | 2020-12-17 | 2024-03-15 | 山东省科学院生物研究所 | Method for testing substrates and products by double-index enzyme electrode detection device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Perez-Olmos et al. | Sequential injection analysis using electrochemical detection: A review | |
| WO2021227542A1 (en) | Immobilized enzyme electrode, and immobilized enzyme sensor and enzyme membrane anti-interference detection method therefor | |
| Graber et al. | The use of chemical sensors in industry | |
| Holzberg et al. | Sensors for biomanufacturing process development: facilitating the shift from batch to continuous manufacturing | |
| US10078077B2 (en) | Apparatus for automated determining of at least two different process parameters | |
| Dixon et al. | The control and measurement of ‘CO2’during fermentations | |
| Mor et al. | Assay of glucose using an electrochemical enzymatic sensor | |
| CN214310287U (en) | Double-index enzyme electrode detection device | |
| Phillips | “Novel” sensors for the monitoring of fermentation processes | |
| CN105738361B (en) | Permanganate index automatic analyzer and analysis method in water | |
| Zhang et al. | Equipment-free quantitative determination of urea based on paper-based sensor via urease-mediated chitosan viscosity change | |
| CN112505122B (en) | Method for testing substrates and products by double-index enzyme electrode detection device | |
| Albanese et al. | Winemaking process monitoring based on a biosensor automatic system | |
| JP2015087293A (en) | Measuring kit and measuring method of glyco-albumin | |
| CN101344502A (en) | Method for measuring malic acid-lactic acid fermentation quality of wine | |
| CN105158310A (en) | Microfluidic detection chip based on micropore electrode and application thereof | |
| Meyerhoff et al. | Polymer-membrane electrode-based potentiometric sensing of ammonia and carbon dioxide in physiological fluids. | |
| CN115184441B (en) | Multi-channel enzyme electrode on-line detection device and method in fermentation production process | |
| US20150369775A1 (en) | Electroanalitical system | |
| CN211955306U (en) | Immobilized enzyme electrode and immobilized enzyme sensor | |
| Meyerhoff et al. | Simultaneous enzymatic/electrochemical determination of glucose and l‐glutamine in hybridoma media by flow‐injection analysis. | |
| JPS5861459A (en) | Analyzing device for creatine and creatinine | |
| AraÚJO et al. | A new approach to dialysis in sequential injection systems: spectrophotometric determination of L (+)-lactate in wines | |
| Danielsson | Fermentation monitoring | |
| Vojinović et al. | Ex situ bioprocess monitoring techniques |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |