CN103235016B - A kind of glucose biological sensor for on-line checking - Google Patents
A kind of glucose biological sensor for on-line checking Download PDFInfo
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- CN103235016B CN103235016B CN201310144353.4A CN201310144353A CN103235016B CN 103235016 B CN103235016 B CN 103235016B CN 201310144353 A CN201310144353 A CN 201310144353A CN 103235016 B CN103235016 B CN 103235016B
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 36
- 239000008103 glucose Substances 0.000 title claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 104
- 108090000790 Enzymes Proteins 0.000 claims abstract description 54
- 102000004190 Enzymes Human genes 0.000 claims abstract description 54
- 239000000872 buffer Substances 0.000 claims abstract description 39
- 238000006243 chemical reaction Methods 0.000 claims abstract description 32
- 238000002347 injection Methods 0.000 claims abstract description 25
- 239000007924 injection Substances 0.000 claims abstract description 25
- 238000003760 magnetic stirring Methods 0.000 claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 8
- 229940088598 enzyme Drugs 0.000 claims description 51
- 239000000463 material Substances 0.000 claims description 9
- 239000004696 Poly ether ether ketone Substances 0.000 claims description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 8
- 229920002530 polyetherether ketone Polymers 0.000 claims description 8
- 108010015776 Glucose oxidase Proteins 0.000 claims description 6
- 239000004366 Glucose oxidase Substances 0.000 claims description 6
- 229940116332 glucose oxidase Drugs 0.000 claims description 6
- 235000019420 glucose oxidase Nutrition 0.000 claims description 6
- 230000005611 electricity Effects 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 4
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
- 229910052737 gold Inorganic materials 0.000 claims description 4
- 239000010931 gold Substances 0.000 claims description 4
- 229910002804 graphite Inorganic materials 0.000 claims description 4
- 239000010439 graphite Substances 0.000 claims description 4
- 229910052697 platinum Inorganic materials 0.000 claims description 4
- 229920002492 poly(sulfone) Polymers 0.000 claims description 4
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 238000002848 electrochemical method Methods 0.000 claims 1
- 238000003487 electrochemical reaction Methods 0.000 abstract description 4
- 238000004401 flow injection analysis Methods 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 abstract description 3
- 230000004151 fermentation Effects 0.000 abstract description 3
- 239000000376 reactant Substances 0.000 abstract description 3
- 238000013019 agitation Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 210000004243 sweat Anatomy 0.000 description 5
- 239000004020 conductor Substances 0.000 description 4
- 239000007769 metal material Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 150000002303 glucose derivatives Chemical class 0.000 description 3
- 230000027756 respiratory electron transport chain Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of glucose biological sensor for on-line checking, belongs to biosensor technology field.It includes working electrode (1), to electrode (2), reference electrode (3), buffer and enzyme liquid entrance (4), liquid outlet (5), buffer and liquid entrance to be measured (6), reaction tank (7), magnetic stirring rotator (8), electrode fixed body (9), external structure body (10), enzyme liquid quantitative injection device (11), liquid quantitative injection device to be measured (14).The present invention uses calibrated shot mode directly by enzyme liquid and liquid calibrated shot to be measured to reaction tank, improves detection speed and the degree of accuracy of sensor；The built-in isolated electromagnetic agitation rotor of reaction tank so that reactant electrochemical reaction more quickly fully, and then improves detection sensitivity；Conveniently be combined with FIA Flow Injection Analysis System, sensor can be realized and carry out online high pressure steam sterilization with fermentation tank simultaneously, easily realize glucose on-line checking.
Description
Technical field
The present invention relates to the glucose detection technology of biosensor formula, be one comprehensively use physics, chemistry, biology,
Electronics for detecting the biosensor of glucose, specifically, be a kind of to be applicable to on-line checking towards sweat
Glucose biological sensor, belong to field of biosensors.
Background technology
1962, Clark and Lyons proposed the concept of " enzyme electrode Enzyme electrode " for the first time, and this has
The event of milestone significance is considered as the initial of biosensor development, and the most therefore Clark obtains " father of biosensor "
Title.Within 1967, Updike and Hicks is successfully made for first glucose enzyme electrode biosensor according to this principle.
The principle of glucose enzyme electrode biosensor is an electrochemical reaction process, as a example by glucoseoxidase i.e.
There is electrochemical reaction, the electronics of generation in glucoseoxidase and glucose in reaction tank, forms electric current, testing circuit lead to
Cross electrode and obtain current signal or voltage signal, thus realize the detection of glucose.According to the difference of electron transfer mediator used,
Glucose enzyme electrode biosensor is segmented into three generations.First generation biosensor is using oxygen molecule or hydrogen peroxide as enzyme
And electron transfer mediators between electrode, the size of current of generation relation proportional to oxygen concentration.The second filial generation uses artificial electron's transmission
The agent such as potassium ferricyanide/potassium ferrocyanide, Ferrocene and its derivative, dye molecule, organic conductive salt, metal phthalocyanine etc. are as electricity
Sub-transfer medium, artificial electron's transfer medium redox reaction on electrode is reversible.The third generation need not electronics and passes
Pass agent, electron transmission directly occurs between electrode and enzyme.
In sweat, in order to prevent microbiological contamination, online glucose biological sensor and and reactor between pipeline must be able to
Bear high-temp steam sterilizing, to avoid its hydraulic performance decline or forfeiture after sterilizing, so glucose biological sensor is mainly at present
Off-line type, online also in the experimental stage.But, glucose be reactant common in fermentation process and in
Between product, in sweat, the control to concentration of glucose can be directly connected to products collection efficiency and quality.Thus concentration of glucose
Real-time online detection be extremely important and necessary.Therefore, present highly desirable a kind of being applicable to for sweat
Glucose biological sensor line, quick, reliable, easy to use.
Summary of the invention
The invention provides a kind of glucose biological sensor being applicable to on-line checking towards sweat.
This glucose biological sensor, it is characterised in that: this sensor includes: working electrode 1, to electrode 2, reference electrode
3, buffer and enzyme liquid entrance 4, liquid outlet 5, buffer and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring rotator 8, electrode are solid
Determining body 9, external structure body 10, enzyme liquid quantitative injection device 11, liquid quantitative injection device 14 to be measured, concrete annexation is:
Described working electrode 1, electrode 2 and reference electrode 3 are fixed in electrode fixed body 9；Electrode fixed body 9 is fixed on
In external structure body 10, external structure body 10 and electrode fixed body 9 constitute reaction tank 7；Reaction tank 7 is built-in with magnetic stirring rotator 8；
Buffer and enzyme liquid entrance 4, buffer and liquid entrance 6 to be measured, liquid outlet 5 is had on electrode fixed body 9；Described
Buffer and enzyme liquid entrance 4 are connected with enzyme liquid quantitative injection device 11 and buffer bottle；Described buffer and liquid entrance to be measured 6 with
Liquid quantitative injection device 14 to be measured and buffer bottle connect；Described liquid outlet 5 is connected with waste liquid bottle；
Working electrode 1 is cylindrically shaped or annular, is made up of metal material or conductive material.
Cylindrically shaped or the annular to electrode 2, is made up of metal material or conductive material.
Reference electrode 3 is cylindrically shaped, annular, and material is Ag/AgCl.
Electrode fixed body 9 is shaped as the cylinder that bottom is cut, is made up of high temperature resistant nonmetallic materials.
External structure body 10 is made up of high temperature resistant nonmetallic materials.
Described glucose biological sensor uses enzyme liquid and liquid calibrated shot mode to be measured.
The invention have the advantage that
Enzyme exists in the way of enzyme liquid, is i.e. dissolved in buffer by enzyme and additive and makes, simple to operate；
Enzyme exists in the enzyme liquid mode of " fresh ", independent with liquid to be measured, not by ectocine so that sensor need not frequency
Numerous calibration；
Changing different types of enzyme liquid, can detect different material, i.e. one sensor can detect many kinds of substance；
Liquid to be measured obtains by adding drainage screen before sampling pipe, saves liquid dialysis part to be measured in sensor, to simplify
Sensor construction；
Testing cost is low, enzyme liquid cost about 0.01 yuan used by single；
Use calibrated shot mode directly by enzyme liquid and liquid calibrated shot to be measured to reaction tank, improve the inspection of sensor
Degree of testing the speed and degree of accuracy；
The built-in isolated electromagnetic agitation rotor of reaction tank so that reactant electrochemical reaction is more quickly abundant, Jin Erti
High detection sensitivity；
Conveniently be combined with FIA Flow Injection Analysis System, sensor can be realized and carry out online high pressure steaming with fermentation tank simultaneously
Vapour sterilizing, easily realizes glucose on-line checking；
Inventive structure is the compactest, easy to process, it is easy to mass production.
Accompanying drawing explanation
Fig. 1 is the agent structure schematic diagram of the glucose biological sensor for on-line checking.
Fig. 2 is the structural representation of enzyme liquid quantitative injection device in this glucose biological sensor.
Fig. 3 is the structural representation of liquid quantitative injection device to be measured in this glucose biological sensor.
In figure: 1, working electrode, 2, to electrode, 3, reference electrode, 4, buffer and enzyme liquid entrance, 5, liquid outlet, 6,
Buffer and liquid entrance to be measured, 7, reaction tank, 8, magnetic stirring rotator, 9, electrode fixed body, 10, external structure body, 11, enzyme liquid quantitative
Injection device, 12, electromagnetism six-way valveI, 13, quantitative loopI, 14, liquid liquid quantitative injection device to be measured, 15, electromagnetism six-way valveII,
16, quantitative loopII。
Detailed description of the invention
As shown in Figure 1, Figure 2 and Figure 3, present configuration is by working electrode 1, to electrode 2, reference electrode 3, buffer and enzyme
Liquid entrance 4, liquid outlet 5, buffer and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring rotator 8, electrode fixed body 9, external structure
Body 10.
Glucose biological sensor for on-line checking, it is characterised in that: this sensor includes: working electrode 1, to electricity
Pole 2, reference electrode 3, buffer and enzyme liquid entrance 4, liquid outlet 5, buffer and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring
Rotor 8, electrode fixed body 9, external structure body 10, enzyme liquid quantitative injection device 11, liquid quantitative injection device 14 to be measured, specifically connect
Relation is:
Described working electrode 1, three electricity that electrode 2 and reference electrode 3 are fixed on electrode fixed body 9 with epoxy resin
In hole, pole；Electrode fixed body 9 is fixed in external structure body 10 by spiral card cap, and external structure body 10 is constituted with electrode fixed body 9
Reaction tank 7；Reaction tank 7 is built-in with magnetic stirring rotator 8；
Buffer and enzyme liquid entrance 4, buffer and liquid entrance 6 to be measured, liquid outlet 5 is had on electrode fixed body 9；Described
Buffer and enzyme liquid entrance 4 are connected with enzyme liquid quantitative injection device 11 and buffer bottle；Described buffer and liquid entrance to be measured 6 with
Liquid quantitative injection device 14 to be measured and buffer bottle connect；Described liquid outlet 5 is connected with waste liquid bottle；
Enzyme liquid quantitative injection device 11 is by electromagnetism six-way valveI12 and quantitative loopI13 are constituted, quantitative loopI13 two ends are connected to
Electromagnetism six-way valveI12 any diagonal angle mouths.Electromagnetism six-way valveI12 a bites therein are connected to buffer and enzyme liquid entrance 4.
Liquid quantitative injection device 14 to be measured is by electromagnetism six-way valveII15 and quantitative loopII16 are constituted, quantitative loopII16 two ends are even
It is connected to electromagnetism six-way valveII15 any diagonal angle mouths.Electromagnetism six-way valveII15 a bites therein are connected to buffer and enzyme liquid entranceII6。
Wherein, described working electrode 1, electrode 2 and reference electrode 3 are fixed in electrode fixed body 9, together constitute electricity
Chemical measurement three-electrode system.
Working electrode 1 is not cylindrically shaped, but is not limited to cylinder, can be by metal material or conductive material, such as but not
It is limited to: platinum, gold, silver, graphite etc. are made.
Cylindrically shaped to electrode 2, but it is not limited to cylinder, such as but can not limited by metal material or conductive material
In: platinum, gold, silver, graphite etc. are made.
Reference electrode 3 is cylindrically shaped, but is not limited to cylinder, can be made up of Ag/AgCl.
Electrode fixed body 9 is shaped as the cylinder that bottom is cut, such as but can not limited by high temperature resistant nonmetallic materials
In: polyether-ether-ketone PEEK, polysulfones etc. are made.
External structure body 10 can be such as but not limited to by high temperature resistant nonmetallic materials: polyether-ether-ketone PEEK, polysulfones etc. are made.
Magnetic stirring rotator 8 is used for stirring liquid to be measured and enzyme liquid, make liquid to be measured and enzyme liquid in reaction tank 7 quickly, the most mixed
Close, can be such as but not limited to by the nonmetallic materials of band magnetic core: the rotors such as polyether-ether-ketone PEEK, polysulfones are made.
Before sensor measurement sample, with buffer solution for cleaning reaction tank 7；Then, liquid to be measured is quantitatively noted by liquid to be measured
Injection device is introduced into reaction tank 7, starts magnetic stirring rotator；Then, by enzyme liquid quantitative injection device by glucoseoxidase liquid
Being expelled in reaction tank 7, in reaction tank 7, glucoseoxidase liquid reacts under three-electrode system effect with glucose,
Producing current signal, working electrode 1 draw, this current signal, by the I/V translation circuit in three electrode detection circuit, becomes
Voltage signal, then amplifies voltage signal, filters, obtain a voltage signal proportional to concentration of glucose to be measured, this
Voltage signal magnitude i.e. represents liquid concentration of glucose to be measured.
Claims (4)
1. the glucose biological sensor for on-line checking, it is characterised in that: this sensor include working electrode (1),
To electrode (2), reference electrode (3), buffer and enzyme liquid entrance (4), liquid outlet (5), buffer and liquid entrance to be measured (6),
Reaction tank (7), magnetic stirring rotator (8), electrode fixed body (9), external structure body (10), enzyme liquid quantitative injection device (11), to be measured
Liquid quantitative injection device (14)；
Described working electrode (1), electrode (2) and reference electrode (3) are fixed on electrode fixed body (9) in；Electrode fixed body (9)
Being fixed in external structure body (10), external structure body (10) constitutes reaction tank (7) with electrode fixed body (9)；In reaction tank (7) built-in
There is magnetic stirring rotator (8)；
Buffer and enzyme liquid entrance (4), buffer and liquid entrance to be measured (6), liquid outlet (5) is had on electrode fixed body (9)；
Described buffer and enzyme liquid entrance (4) are connected with enzyme liquid quantitative injection device (11) and buffer bottle；Described buffer and to be measured
Liquid entrance (6) is connected with liquid quantitative injection device (14) to be measured and buffer bottle；Described liquid outlet (5) is connected with waste liquid bottle；
Electrode fixed body (9) is shaped as the cylinder that bottom is cut, is made up of polyether-ether-ketone PEEK, polysulfones；
External structure body (10) is made up of high temperature resistant nonmetallic materials；
Use calibrated shot mode directly from buffer and enzyme liquid entrance (4) and buffer and liquid entrance to be measured (6) by enzyme liquid with
Liquid calibrated shot to be measured is in reaction tank；Enzyme liquid is dissolved in buffer by enzyme and additive and makes, and liquid to be measured adds before sampling pipe
Drainage screen obtains；
Before sensor measurement sample, with buffer solution for cleaning reaction tank (7)；Then, liquid to be measured passes through liquid calibrated shot to be measured
Device is introduced into reaction tank (7), starts magnetic stirring rotator；Then, by enzyme liquid quantitative injection device by glucoseoxidase liquid
Being expelled in reaction tank (7), in reaction tank (7), glucoseoxidase liquid and glucose occur instead under three-electrode system effect
Should, producing current signal, working electrode (1) draw, this current signal is by the I/V conversion electricity in three electrode detection circuit
Road, becomes voltage signal, then amplifies voltage signal, filters, obtains a voltage proportional to concentration of glucose to be measured
Signal, this voltage signal magnitude i.e. represents liquid concentration of glucose to be measured；
Enzyme liquid is dissolved in buffer by enzyme and additive and makes, and enzyme liquid is independent with liquid to be measured；
Enzyme liquid quantitative injection device (11) is made up of electromagnetism six-way valve I (12) and quantitative loop I (13), and quantitative loop I (13) two ends are even
It is connected to electromagnetism six-way valve I (12) arbitrarily diagonal angle mouth；Electromagnetism six-way valve I (12) a bite therein is connected to buffer and enzyme liquid entrance
(4)；
Liquid quantitative injection device (14) to be measured is made up of electromagnetism six-way valve II (15) and quantitative loop II (16), quantitative loop II (16) two
End is connected to electromagnetism six-way valve II (15) arbitrarily diagonal angle mouth；Electromagnetism six-way valve II (15) a bite therein is connected to buffer and treats
Survey liquid entrance (6).
A kind of glucose biological sensor for on-line checking the most according to claim 1, it is characterised in that: reaction tank
Being made up of external structure body and electrode fixed body, reaction tank volume is 400 μ L to 1000 μ L, and magnetic stirring rotator body is placed in reaction tank
In, volume is 200 μ L～500 μ L, and less than the 50% of reaction tank volume.
A kind of glucose biological sensor for on-line checking the most according to claim 1, it is characterised in that: magnetic stirs
Rotor (8) is made up of the corrosion-and high-temp-resistant nonmetallic materials of band magnetic core；Magnet in magnetic stirring rotator (8) is outer with sensor micro-
Magnet of the same race fixing on type DC motor rotor is parallel, and magnetic stirring rotator (8) rotates along with electric machine rotation, realizes non-with this
Contact stirring.
A kind of glucose biological sensor for on-line checking the most according to claim 1, it is characterised in that: described work
Make electrode (1), electrode (2) and reference electrode (3) be fixed in electrode fixed body (9), together constitute electrochemical measurement three
Electrode system；
Working electrode (1) is shaped as the cylinder that bottom is cut, is made up of platinum, gold, silver, graphite；
Electrode (2) is shaped as the cylinder that bottom is cut, is made up of platinum, gold, silver, graphite；
Reference electrode (3) is shaped as the cylinder that bottom is cut, is made up of Ag/AgCl.
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| CN201310144353.4A CN103235016B (en) | 2013-04-23 | 2013-04-23 | A kind of glucose biological sensor for on-line checking |
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| CN201310144353.4A CN103235016B (en) | 2013-04-23 | 2013-04-23 | A kind of glucose biological sensor for on-line checking |
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| CN104777192B (en) * | 2015-03-18 | 2017-06-20 | 浙江工商大学 | A kind of detection method of food sugariness |
| CN105319260B (en) * | 2015-11-05 | 2017-10-31 | 北京农业信息技术研究中心 | The online glucose sensing approach of plant and device based on microelectrode biosensing technology |
| CN107202830A (en) * | 2017-06-15 | 2017-09-26 | 南京工业大学 | A kind of device detected based on prussian blue film bioelectrode |
| CN107271514A (en) * | 2017-06-15 | 2017-10-20 | 南京工业大学 | It is a kind of based on detection method of the prussian blue film bioelectrode to glucose |
| CN108896340A (en) * | 2018-03-23 | 2018-11-27 | 吉林农业大学 | A kind of multiple pipe drilling core bit for soil drill sampling |
| CN110523355A (en) * | 2018-05-23 | 2019-12-03 | 青岛千和电子有限公司 | Oxidation reaction generator |
| CN109342734A (en) * | 2018-12-20 | 2019-02-15 | 中山大学附属第八医院（深圳福田） | A kind of portable blood sugar detector |
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| JP3318044B2 (en) * | 1993-05-28 | 2002-08-26 | 有限会社 岩佐事務所 | FIA biosensor |
| SE510733C2 (en) * | 1995-01-03 | 1999-06-21 | Chemel Ab | Chemical sensor based on interchangeable recognition component and its use |
| CN1152253C (en) * | 1999-02-23 | 2004-06-02 | 王劲松 | Detecting method and apparatus for yeast concentration (measuring bioconcentration) |
| CN102141535B (en) * | 2010-12-31 | 2013-10-02 | 北京工业大学 | Enzyme-injection glucose sensing on-line analyzer |
| CN102175739A (en) * | 2010-12-31 | 2011-09-07 | 北京工业大学 | Enzyme injection type glucose biosensor |
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