CN107202830A - A kind of device detected based on prussian blue film bioelectrode - Google Patents
A kind of device detected based on prussian blue film bioelectrode Download PDFInfo
- Publication number
- CN107202830A CN107202830A CN201710451456.3A CN201710451456A CN107202830A CN 107202830 A CN107202830 A CN 107202830A CN 201710451456 A CN201710451456 A CN 201710451456A CN 107202830 A CN107202830 A CN 107202830A
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- Prior art keywords
- pond body
- prussian blue
- blue film
- sample cell
- cavity
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- 229960003351 prussian blue Drugs 0.000 title claims abstract description 52
- 239000013225 prussian blue Substances 0.000 title claims abstract description 52
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 238000007789 sealing Methods 0.000 claims abstract description 41
- 239000000741 silica gel Substances 0.000 claims abstract description 34
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 34
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000003760 magnetic stirring Methods 0.000 claims abstract description 19
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims description 28
- 230000005540 biological transmission Effects 0.000 claims description 16
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- SPFMQWBKVUQXJV-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;hydrate Chemical compound O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O SPFMQWBKVUQXJV-BTVCFUMJSA-N 0.000 claims description 3
- 229960000673 dextrose monohydrate Drugs 0.000 claims description 3
- 238000007667 floating Methods 0.000 claims description 3
- 241001062009 Indigofera Species 0.000 claims description 2
- 238000013019 agitation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 20
- 229960001031 glucose Drugs 0.000 description 20
- 239000008103 glucose Substances 0.000 description 20
- 238000001514 detection method Methods 0.000 description 14
- 239000000463 material Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 238000004817 gas chromatography Methods 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 229920005479 Lucite® Polymers 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000011810 insulating material Substances 0.000 description 3
- 239000004926 polymethyl methacrylate Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 210000003739 neck Anatomy 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- YKHQSWIVNHQJSW-UHFFFAOYSA-N iron;oxalonitrile Chemical compound [Fe].N#CC#N YKHQSWIVNHQJSW-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
Abstract
The invention discloses a kind of device detected based on prussian blue film bioelectrode, including sample cell, magnetic stirring apparatus, support, the sample cell is fixedly installed on the top of the support, and the magnetic stirring apparatus is fixedly installed on the lower section of the support；The sample cell includes hollow pond body, hollow screws lid, silica gel sealing pad, the top exterior walls of the pond body are used with the bottom interior wall for screwing lid and are threadedly coupled, the pond body is internally provided with hollow cavity, the pond body inwall of the parcel cavity, the silica gel sealing pad nesting is arranged in the cavity of the pond body, it is provided with the cavity on solution, the silica gel sealing pad and is fitted with prussian blue film bioelectrode sensor；The bottom of the pond body is respectively arranged with water inlet, delivery port, the outer end of the water inlet, the outer end of the delivery port are respectively communicated with the peristaltic pump for Inlet and outlet water, the cavity of the pond body and are additionally provided with the driven rotor for agitating solution being engaged with the magnetic stirring apparatus.The present invention is simple to operate, is easily installed and dismantles, good airproof performance, strong antijamming capability is with low cost, beneficial to large-scale production.
Description
Technical field
The present invention relates to a kind of device detected based on prussian blue film bioelectrode, belong to detection means technical field.
Background technology
In fermentation industry, glucose is the primary carbon source of many tunnings, the direct shadow of concentration of glucose of zymotic fluid
Ring the yield and quality to tunning.In earlier fermentation, if concentration of glucose is too high, resistance easily is produced to thalli growth
Hold back, suppress and restriction effect；After fermentation the phase, if concentration of glucose is too low, thalli growth and Product formation can be limited.Mesh
Often add glucose using process streams in preceding industry, on-line checking concentration of glucose is aided in, so as to realize the stability contorting of glucose.
At present the method for detection concentration of glucose mainly have high performance liquid chromatography, it is gas chromatography, AAS, near
Infrared, electrochemica biological sensor etc..High performance liquid chromatography application is the most wide, but it needs expensive large-scale instrument
Equipment, and it is not readily portable；Gas chromatography precision is of a relatively high, but silicon ether processing, complex steps, behaviour need to be carried out to glucose
Make complicated；AAS need to add developer, can pollute glucose solution, and operating process is comparatively laborious, and accuracy of detection is low；Closely
Infrared foundation can carry out qualitative and quantitative analysis on the basis of certain algorithm to material, but its measurement accuracy is relatively low；Electrochemical student
Thing sensor, has the advantages that linear detection range is wide, cost is low, easy to operate, sensitivity is high, possess excellent repeatability,
Stability and antijamming capability, possess good application prospect in glucose detection.
Prussian blue (PB) is a kind of inorganic iron cyanogen complex, due to its excellent electrochemical reversibility, stability
And to H2O2With specific electro catalytic activity, it is widely used in enzyme electrochemica biological sensor.For prussian blue film life
Thing electrode need to design a detection means.
The content of the invention
At present the method for detection glucose mainly have high performance liquid chromatography, it is gas chromatography, AAS, near red
Outside, electrochemica biological sensor etc..Wherein, high performance liquid chromatography application is the most wide, but it needs the large-scale of costliness
Instrument and equipment, and it is not readily portable；Gas chromatography precision is of a relatively high, but silicon ether processing need to be carried out to glucose, and operation is multiple
It is miscellaneous；AAS need to add developer, can pollute glucose solution, and operating process is comparatively laborious, and accuracy of detection is low；Near-infrared
Qualitative and quantitative analysis can be carried out on the basis of certain algorithm to material by setting up, but its measurement accuracy is relatively low；What the present invention was used
It is electrochemica biological biology sensor, it has the advantages that, and linear detection range is wide, cost is low, easy to operate, sensitivity is high,
Possesses good application prospect in glucose detection.Currently used for of all kinds on the apparatus structure of liquid detecting, functionally
Varied, the design of device is based on specific detection device, therefore, and the design of detection means cannot rely upon fixed set
Meter scheme.
The present invention be directed to a kind of device of examination of glucose concentration of prussian blue film bioelectrode Sensor Design, its
Have the advantages that linear detection range is wide, cost is low, easy to operate, sensitivity is high, possess excellent repeatability, stability and resist
Interference performance, possesses good application prospect in glucose detection.
The present invention be directed to a kind of device of examination of glucose concentration of prussian blue film bioelectrode design.The device bag
Include three parts：One sample cell, a magnetic stirring apparatus, a support.The device is simple to operate, is easily installed and dismantles, sealing
Good, strong antijamming capability is with low cost, beneficial to large-scale production.
The present invention is adopted the following technical scheme that：A kind of device detected based on prussian blue film bioelectrode, its feature is existed
In including sample cell, magnetic stirring apparatus, support, the sample cell are fixedly installed on the top of the support, and the magnetic force is stirred
Mix the lower section that device is fixedly installed on the support；
The sample cell include hollow pond body, it is hollow screw lid, silica gel sealing pad, the top exterior walls of the pond body and
The bottom interior wall for screwing lid is using threaded connection, and the pond body is internally provided with hollow cavity, wraps up the cavity
Pond body inwall, the silica gel sealing pad nesting is arranged in the cavity of the pond body, and solution is provided with the cavity, described
Prussian blue film bioelectrode sensor is fitted with silica gel sealing pad；The bottom of the pond body is respectively arranged with water inlet, gone out
The mouth of a river, the outer end of the water inlet, the outer end of the delivery port are respectively communicated with the peristaltic pump for Inlet and outlet water, the institute of the pond body
State the driven rotor for agitating solution for being additionally provided with and being engaged with the magnetic stirring apparatus in cavity.
As a kind of preferred embodiment, the cavity of pond body is designed using inverted round stage, prevents occurring during rotor agitating solution
Substantial amounts of whirlpool and disturb prussian blue film bioelectrode normal operation of sensor, possess certain antijamming capability.
As a kind of preferred embodiment, water inlet is arranged at the top of delivery port, is conducive to the solution in sample cell
Emptying；Water inlet is connected by external water inlet pipe with peristaltic pump, and delivery port is connected by external outlet pipe with peristaltic pump
It is logical.
As a kind of preferred embodiment, the bottom of pond body is provided with the bottom of pond of cylindrical shape, for being connected with support.
As a kind of preferred embodiment, screw that lid includes centre hollow out screws lid, screws in the middle of the top of lid
Hollow out cylinder inwall is provided with, the lower inner wall for screwing lid carves the connection internal thread for being provided with and being used for being connected with pond body；Screw
Lid is used for realizing the sealing of sample cell, and the design of hollow hollow out is partly in order to by prussian blue film bioelectrode sensor
The wiring of electrode draw, on the other hand convenient injection solution.
As a kind of preferred embodiment, the centre of silica gel sealing pad offers electrode strip seam, and electrode strip seam is used for inserting
The injection orifice for injectable dextrose monohydrate solution is additionally provided with prussian blue film bioelectrode sensor, silica gel sealing pad.
As a kind of preferred embodiment, magnetic stirring apparatus includes direct current generator, power rotor, motor transmission shaft, rotation
Ask disk, carriage mounting plate, and direct current generator is fixedly installed on the bottom of carriage mounting plate, and motor transmission shaft is arranged at support installation
The top of plate, the output end of direct current generator coordinates with the bottom of motor transmission shaft and coupled, and top and the rotation of motor transmission shaft are held in the palm
The centre of disk is fixedly connected, and rotary-tray rotates in same direction with motor transmission shaft, and power rotor is symmetrically fixedly installed on rotary-tray
On, power rotor coordinates with the driven rotor magnetic force in sample cell, and the both sides of carriage mounting plate are fixedly connected with the both sides of support.
As a kind of preferred embodiment, the bottom of pond being engaged with the bottom of sample cell is offered at the top of support and is installed
Groove, the both sides of support are offered respectively is fastenedly connected hole, and the both sides of support are fixed by being fastenedly connected hole with magnetic stirring apparatus
Connection.
As a kind of preferred embodiment, prussian blue film bioelectrode sensor is three electrode sensors.
As a kind of preferred embodiment, driven rotor is the floating rotor of embedded magnet outer wrapping silica gel.
The beneficial effect that the present invention is reached：First, the present invention is simple to operate, is easily installed and dismantles, while convenient warp
Often change the electrode of prussian blue film bioelectrode sensor；Second, of the invention screws cylinder of the lid for middle hollow out, rotation
Tight lid can realize the sealing of sample cell, and on the one hand hollow design is can be by three of prussian blue film bioelectrode sensor
The wiring of electrode is drawn, on the other hand convenient injection glucose sugar juice；3rd, good airproof performance of the invention, using silica gel sealing
Pad has elasticity and wearability well, and it is placed in pond body top open part, is screwed if lid will be screwed with pond body, silica gel sealing pad
Uniform force, by prussian blue film bioelectrode sensor clamping, can realize the sealing of sample cell so that biology sensor
Electrode will not be soaked by solution；4th, strong antijamming capability of the invention, the cavity of sample cell uses the design of inverted round stage,
It can prevent occurring substantial amounts of whirlpool during driven rotor agitating solution in upper solution and disturb normal operation of sensor；The
Five, support of the invention uses insulating materials, can prevent the interference to power rotor and driven rotor, so as to avoid influence from examining
Survey concentration；6th, be also drilled with two intake-outlets on sample cell of the invention, water inlet upper, delivery port under, be conducive to by
Solution emptying in sample cell, it is not necessary to which the operation such as cleaning, sample introduction can be completed to sample cell dismounting；7th, cost of the invention
It is cheap, beneficial to production in enormous quantities.
Brief description of the drawings
Fig. 1 is the structural representation of one embodiment of the sample cell of the present invention.
Fig. 2 is the structural representation of one embodiment of the magnetic stirring apparatus of the present invention.
Fig. 3 is the structural representation of one embodiment of the support of the present invention.
Fig. 4 is the structural representation of one embodiment of the silica gel sealing pad of the present invention.
Fig. 5 is the structural representation of one embodiment of the prussian blue film biology sensor of the present invention.
Fig. 6 is the structural representation of the sectional view of one embodiment of the hollow pond body of the present invention.
Fig. 7 is the structural representation of Fig. 6 top view.
Fig. 8 is the structural representation of the sectional view of one embodiment for screwing lid of the present invention.
Fig. 9 is the structural representation of Fig. 8 top view.
The implication marked in figure：1- sample cells, 11- connection external screw threads, 12- pond bodies, 13- pond body inwalls, 14- water inlets,
15- delivery ports, 16- bottom of pond, 17- water inlet pipes, 18- outlet pipes；2- screws lid, and 21- hollow out cylinder inwalls, 22- screws lid,
23- connection internal threads；3- magnetic stirring apparatuses, 31- direct current generators, 32- power rotors, 33- motor transmission shafts, 34- rotation supports
Disk, 35- carriage mounting plates；4- supports, 41- bottom of pond mounting grooves, 42- is fastenedly connected hole；5- silica gel sealing pads, 51- electrode strips seam,
52- injection orifices, 6- prussian blue film bioelectrode sensors.
Embodiment
The invention will be further described below in conjunction with the accompanying drawings.Following examples are only used for clearly illustrating the present invention
Technical scheme, and can not be limited the scope of the invention with this.
Fig. 1 is the structural representation of one embodiment of the sample cell of the present invention.Fig. 2 is the magnetic stirring apparatus of the present invention
One embodiment structural representation.Fig. 3 is the structural representation of one embodiment of the support of the present invention.The present invention is proposed
A kind of device detected based on prussian blue film bioelectrode, including sample cell 1, magnetic stirring apparatus 3, support 4, sample cell 1
The top of support 4 is fixedly installed on, magnetic stirring apparatus 3 is fixedly installed on the lower section of support 4；Sample cell 1 includes hollow pond
Body 12, it is hollow screw lid 2, silica gel sealing pad 5, the top exterior walls of pond body 12 and screw the bottom interior wall of lid 2 using screw thread company
Connect, pond body 12 is internally provided with hollow cavity, wraps up the pond body inwall 13 of cavity, and the nesting of silica gel sealing pad 5 is arranged at pond
In the cavity of body 12, it is provided with cavity on solution, silica gel sealing pad 5 and is fitted with prussian blue film bioelectrode sensor 6；Pond
The bottom of body 12 is respectively arranged with water inlet 14, delivery port 15, and outer end, the outer end of delivery port 15 of water inlet 14 are respectively communicated with use
To be additionally provided with the agitating solution that is used for being engaged with magnetic stirring apparatus 3 in the peristaltic pump of Inlet and outlet water, the cavity of pond body 12 and use
Driven rotor.
As a kind of preferred embodiment, the cavity of pond body 12 is designed using inverted round stage, prevents from going out during rotor agitating solution
Show substantial amounts of whirlpool and disturb the normal work of prussian blue film bioelectrode sensor 6, possess certain antijamming capability.
As a kind of preferred embodiment, water inlet 14 is arranged at the top of delivery port 15, is conducive in sample cell 1
Solution emptying；Water inlet 14 is connected by external water inlet pipe 17 with peristaltic pump, and delivery port 15 passes through external outlet pipe 18
It is connected with peristaltic pump.
As a kind of preferred embodiment, the bottom of pond body 12 is provided with the bottom of pond 16 of cylindrical shape, for the phase of support 4
Connection.
As a kind of preferred embodiment, screwing lid 2 includes the top for screwing lid 22, screwing lid 22 of middle hollow out
Centre is provided with hollow out cylinder inwall 21, and the lower inner wall for screwing lid 22 is carved in the connection for being provided with and being used for being connected with pond body 12
Screw thread 23；Screw lid 22 and be used for realizing the sealing of sample cell 1, the design of hollow hollow out is partly in order to by prussian blue film
The wiring of the electrode of bioelectrode sensor 6 is drawn, on the other hand convenient injection solution.
Fig. 4 is the structural representation of one embodiment of the silica gel sealing pad of the present invention.As a kind of preferred embodiment,
The centre of silica gel sealing pad 5 offers electrode strip seam 51, and electrode strip seam 51 is used for inserting prussian blue film bioelectrode sensor
6, the injection orifice 52 for injectable dextrose monohydrate solution is additionally provided with silica gel sealing pad 5.
As a kind of preferred embodiment, magnetic stirring apparatus 3 includes direct current generator 31, power rotor 32, motor transmission
Axle 33, rotary-tray 34, carriage mounting plate 35, direct current generator 31 are fixedly installed on the bottom of carriage mounting plate 35, motor transmission
Axle 33 is arranged at the top of carriage mounting plate 35, and the output end of direct current generator 31 coordinates with the bottom of motor transmission shaft 33 and coupled,
The top of motor transmission shaft 33 and the centre of rotary-tray 34 are fixedly connected, and rotary-tray 34 turns in the same direction with motor transmission shaft 33
Dynamic, power rotor 32 is symmetrically fixedly installed on rotary-tray 34, and power rotor 32 is matched somebody with somebody with the driven rotor magnetic force in sample cell 1
Close, the both sides of carriage mounting plate 35 are fixedly connected with the both sides of support 4.
As a kind of preferred embodiment, the top of support 4 offers the bottom of pond being engaged with the bottom of sample cell 1 and installed
Groove 41, the both sides of support 4 are offered respectively is fastenedly connected hole 42, and the both sides of support 4 are by being fastenedly connected hole 42 and magnetic agitation
Device 3 is fixedly connected.
Fig. 5 is the structural representation of one embodiment of the prussian blue film biology sensor of the present invention.As one kind compared with
Good embodiment, prussian blue film bioelectrode sensor 6 is three electrode sensors.
As a kind of preferred embodiment, driven rotor is the floating rotor of embedded magnet outer wrapping silica gel.
The present invention is further illustrated from specific material, structure and application method separately below.
In terms of material：1. the main body of sample cell 1 is pond body 12 and screws lid 2 using lucite, and its advantage is highly saturating
Bright, it may be observed that sample cell inner case, corrosion-resistant, moisture-proof, good insulation preformance does not interfere with electrode work；2. silica gel sealing
Prussian blue film bioelectrode sensor 6 can be inserted on pad 5, its advantage is on the one hand convenient often to change prussian blue film bioelectrode
The electrode of sensor, on the other hand realizes the sealing of sample cell 1, and silica gel sealing pad 5 has elasticity and wearability, silica gel well
Sealing gasket 5 is placed in pond body 12 and screwed between lid 2, is screwed once lid 2 will be screwed, the uniform force of silica gel sealing pad 5, can be by general Shandong
The clamping of scholar's indigo plant film bioelectrode sensor 6, you can realize the sealing of sample cell 1, while also causing prussian blue film biology to pass
The electrode of sensor 6 will not be wetted, and the silica gel sealing pad 5 is also replaceable into rubber gasket；3. water inlet pipe 17 and outlet pipe 18
Use diameter 3mm stainless steel capillary；4. the support 4 of bottom uses insulating materials, such as plastics, ceramics etc., it is to avoid
Disturb power rotor 32 and driven rotor rotation.
Configuration aspects：1. the design of inverted round stage is used in the cavity of sample cell 1, in order to prevent during driven rotor agitating solution
There is substantial amounts of whirlpool and disturb prussian blue film electrode sensor normal work, possess certain antijamming capability；2. pond body
12 two bottom sides are respectively arranged with water inlet 14 and delivery port 15, and water inlet 14 is upper, and delivery port 15 is under, and delivery port 15 exists
Under advantage be advantageous for solution emptying in sample cell 1, Inlet and outlet water is carried out by peristaltic pump, it is not necessary to be to the dismounting of sample cell 1
The operation such as cleaning, sample introduction can be completed；3. the bottom bottom of pond 16 of pond body 12 is dome cylinder, for the connection with the support 4 of bottom；
4. screw the cylinder that lid 2 is middle hollow out, screw lid 2 can realize the sealing of sample cell 1, hollow design partly in order to
The wiring of three electrodes of prussian blue film bioelectrode sensor 6 is drawn, it is on the other hand convenient to be injected by injection orifice 52
Glucose sugar juice；5. the cavity of sample cell 1 and screwing is screwed by screw thread between lid 2, and a silica gel sealing pad 5, one are added therebetween
Aspect is used for the installation of the electrode of prussian blue film bioelectrode sensor 6, on the other hand realizes sealing, three uses cooperatively
It is simple to operate, it is easily installed and dismantles.
The material selection of support 4：Using insulating materials, to prevent the rotating speed for disturbing power rotor 32 and driven rotor, from
And avoid influenceing the concentration of the detection solution of prussian blue film bioelectrode sensor 6, possess certain antijamming capability.
Configuration aspects：The one diameter groove more slightly larger than the bottom of sample cell 1 (i.e. bottom of pond mounting groove 41) is arranged at the top of support 4, this
Sample prescription is just connected with sample cell 1, plays the fixation to sample cell 1.
Application method：First, two peristaltic pumps are connected on water inlet 14, on delivery port 15, and driven rotor is put into sample
In the cavity in product pond 1, then prussian blue film bioelectrode sensor 6 is inserted on silica gel sealing pad 5, by silica gel sealing pad 5
The top of cavity is placed in, is screwed with the hollow lid 2 that screws, support 4 is placed on the direct current generator 31 of magnetic stirring apparatus 3, and
Sample cell 1 is placed on support 4.
Fig. 6 is the structural representation of the sectional view of one embodiment of the hollow pond body of the present invention.Fig. 7 is Fig. 6 vertical view
The structural representation of figure.Fig. 8 is the structural representation of the sectional view of one embodiment for screwing lid of the present invention.Fig. 9 is Fig. 8
The structural representation of top view.
Material：Pond body 12, screw lid 22 use lucite, water inlet 14 connect water inlet pipe 17, delivery port 15
The outlet pipe 18 of connection uses 3mm stainless steel capillaries.
Structure：Pond body 12 is in appearance three sections of cylinders, and top cylindrical body is the neck of pond body 12, carves outer provided with connection
Screw thread 11, for being connected with screwing lid 2；The cylinder of center section is the cavity of pond body 12, and its pond body inwall 13 is rounding
Platform is designed, for depositing solution；The left and right sides of pond body 12 is drilled with water inlet 14, delivery port 15, and water inlet 14 is in upper, delivery port
15 under；Bottom is that dome cylinder (i.e. bottom of pond 16) is mainly used in being connected with support 4.Screw lid 2 is cylinder in appearance, interior
Portion is the cylinder of two up-small and down-big hollow outs of diameter up and down, and the big cylinder of hollow out, which is provided with, connects internal thread 23, is used for and pond body
The connection external screw thread 11 of 12 necks is connected.
The concrete structure and preparation process of prussian blue film bioelectrode sensor 6 in the present invention refer to entitled
" the Prussian blue film modified screen printing electrode of self assembly builds high-sensitive biosensor ", author for " Peng Jingmeng, Chu Zhenyu,
Shi Lei, Jin Wanqin ", Nanjing University of Technology's journal (natural science edition), 2016,38 (4)：39-45.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, above-mentioned lucite, stainless steel capillary, plastics etc. are also not limited on material；Sample cell is in appearance in structure
It is not limited to cylinder；Without departing from the technical principles of the invention, some improvement and deformation can also be made, these change
Enter and deform and also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of device detected based on prussian blue film bioelectrode, it is characterised in that including sample cell (1), magnetic agitation
Device (3), support (4), the sample cell (1) are fixedly installed on the top of the support (4), the magnetic stirring apparatus (3)
It is fixedly installed on the lower section of the support (4)；
The sample cell (1) includes hollow pond body (12), hollow screws lid (2), silica gel sealing pad (5), the pond body
(12) bottom interior wall that top exterior walls screw lid (2) with described is using being threadedly coupled, and pond body (12) are internally provided with
The pond body inwall (13) of hollow cavity, the parcel cavity, silica gel sealing pad (5) nesting is arranged at the pond body (12)
Cavity in, be provided with the cavity on solution, the silica gel sealing pad (5) be fitted with prussian blue film bioelectrode sensing
Device (6)；The bottom of the pond body (12) is respectively arranged with water inlet (14), delivery port (15), the outer end of the water inlet (14),
The outer end of the delivery port (15) is respectively communicated with the peristaltic pump for Inlet and outlet water, the cavity of the pond body (12) and also set up
There is the driven rotor for agitating solution being engaged with the magnetic stirring apparatus (3).
2. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
The cavity of pond body (12) is designed using inverted round stage, prevents occurring substantial amounts of whirlpool during driven rotor agitating solution and disturb described general
Shandong scholar indigo plant film bioelectrode sensor (6) normal work.
3. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
Water inlet (14) is arranged at the top of the delivery port (15), is conducive to the solution emptying in the sample cell (1)；It is described enter
The mouth of a river (14) is connected by external water inlet pipe (17) with peristaltic pump, and the delivery port (15) passes through external outlet pipe (18)
It is connected with peristaltic pump.
4. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
The bottom of pond body (12) is provided with the bottom of pond (16) of cylindrical shape, for being connected with the support (4).
5. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
Screw lid (2) and be provided with hollow out cylinder in the middle of lid (22), the top for screwing lid (22) including middle screwing for hollow out
Internal wall (21), the lower inner wall for screwing lid (22) carves the connection internal thread for being provided with and being used for being connected with pond body (12)
(23)；It is described to screw the sealing that lid (22) is used for realizing sample cell (1), the effect of the design of hollow hollow out：On the one hand be by
The wiring of the electrode of the prussian blue film bioelectrode sensor (6) is drawn, and is on the other hand conveniently to the sample cell (1)
Cavity in inject solution.
6. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
The centre of silica gel sealing pad (5) offers electrode strip seam (51), and the electrode strip seam (51) is used for inserting the prussian blue film
The injection orifice (52) for injectable dextrose monohydrate solution is additionally provided with bioelectrode sensor (6), the silica gel sealing pad (5).
7. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
Magnetic stirring apparatus (3) includes direct current generator (31), power rotor (32), motor transmission shaft (33), rotary-tray (34), support
Installing plate (35), the direct current generator (31) is fixedly installed on the bottom of the carriage mounting plate (35), the motor transmission shaft
(33) top of the carriage mounting plate (35), output end and the motor transmission shaft of the direct current generator (31) are arranged at
(33) bottom coordinates connection, and the top of the motor transmission shaft (33) and the centre of the rotary-tray (34) are fixedly connected,
The rotary-tray (34) rotates in same direction with the motor transmission shaft (33), and the power rotor (32) is symmetrically fixedly installed on institute
State on rotary-tray (34), the power rotor (32) coordinates with the driven rotor magnetic force in the sample cell (1), described
The both sides of carriage mounting plate (35) are fixedly connected with the both sides of the support (4).
8. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
The bottom of pond mounting groove (41) being engaged with the bottom of the sample cell (1) is offered at the top of support (4), the support (4)
Both sides are offered respectively is fastenedly connected hole (42), and the both sides of the support (4) are fastenedly connected hole (42) and the magnetic by described
Power agitating device (3) is fixedly connected.
9. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that described
Prussian blue film bioelectrode sensor (6) is three electrode sensors.
10. a kind of device detected based on prussian blue film bioelectrode according to claim 1, it is characterised in that institute
State the floating rotor that driven rotor is embedded magnet outer wrapping silica gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710451456.3A CN107202830A (en) | 2017-06-15 | 2017-06-15 | A kind of device detected based on prussian blue film bioelectrode |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710451456.3A CN107202830A (en) | 2017-06-15 | 2017-06-15 | A kind of device detected based on prussian blue film bioelectrode |
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| CN201710451456.3A Pending CN107202830A (en) | 2017-06-15 | 2017-06-15 | A kind of device detected based on prussian blue film bioelectrode |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0017236A1 (en) * | 1979-04-07 | 1980-10-15 | Forschungszentrum Jülich Gmbh | Voltammetric cell and process for manufacturing an appropriate measuring electrode |
| CN1763544A (en) * | 2005-09-30 | 2006-04-26 | 中国科学院长春应用化学研究所 | Be used for the minisize detection tank that high performance liquid chromatography-electrochemical detects |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
| CN105301069A (en) * | 2015-09-30 | 2016-02-03 | 王竞 | Liquid analysis method and device adopting ion-selective-electrode method |
-
2017
- 2017-06-15 CN CN201710451456.3A patent/CN107202830A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0017236A1 (en) * | 1979-04-07 | 1980-10-15 | Forschungszentrum Jülich Gmbh | Voltammetric cell and process for manufacturing an appropriate measuring electrode |
| CN1763544A (en) * | 2005-09-30 | 2006-04-26 | 中国科学院长春应用化学研究所 | Be used for the minisize detection tank that high performance liquid chromatography-electrochemical detects |
| CN103235016A (en) * | 2013-04-23 | 2013-08-07 | 北京工业大学 | Glucose biosensor for online detection |
| CN105301069A (en) * | 2015-09-30 | 2016-02-03 | 王竞 | Liquid analysis method and device adopting ion-selective-electrode method |
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