CN103235016A - Glucose biosensor for online detection - Google Patents
Glucose biosensor for online detection Download PDFInfo
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- CN103235016A CN103235016A CN2013101443534A CN201310144353A CN103235016A CN 103235016 A CN103235016 A CN 103235016A CN 2013101443534 A CN2013101443534 A CN 2013101443534A CN 201310144353 A CN201310144353 A CN 201310144353A CN 103235016 A CN103235016 A CN 103235016A
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 38
- 239000008103 glucose Substances 0.000 title claims abstract description 38
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 88
- 108090000790 Enzymes Proteins 0.000 claims abstract description 48
- 102000004190 Enzymes Human genes 0.000 claims abstract description 48
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 238000003760 magnetic stirring Methods 0.000 claims abstract description 16
- 238000013016 damping Methods 0.000 claims description 34
- 239000012530 fluid Substances 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 13
- 239000004696 Poly ether ether ketone Substances 0.000 claims description 10
- 229920002530 polyetherether ketone Polymers 0.000 claims description 10
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 8
- 239000004020 conductor Substances 0.000 claims description 6
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 5
- 229920002492 poly(sulfone) Polymers 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
- 229910052737 gold Inorganic materials 0.000 claims description 4
- 239000010931 gold Substances 0.000 claims description 4
- 229910002804 graphite Inorganic materials 0.000 claims description 4
- 239000010439 graphite Substances 0.000 claims description 4
- 229910052697 platinum Inorganic materials 0.000 claims description 4
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 3
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 2
- 238000002848 electrochemical method Methods 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000003487 electrochemical reaction Methods 0.000 abstract description 4
- 238000004401 flow injection analysis Methods 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 238000000855 fermentation Methods 0.000 abstract description 3
- 230000004151 fermentation Effects 0.000 abstract description 3
- 239000000376 reactant Substances 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 5
- 230000003139 buffering effect Effects 0.000 abstract 2
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 36
- 210000004243 sweat Anatomy 0.000 description 5
- 108010015776 Glucose oxidase Proteins 0.000 description 4
- 239000004366 Glucose oxidase Substances 0.000 description 4
- 229940116332 glucose oxidase Drugs 0.000 description 4
- 235000019420 glucose oxidase Nutrition 0.000 description 4
- 239000007769 metal material Substances 0.000 description 4
- 150000002303 glucose derivatives Chemical class 0.000 description 3
- 230000027756 respiratory electron transport chain Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a glucose biosensor for online detection, belonging to the technical field of biosensors. The glucose biosensor comprises a working electrode (1), a counter electrode (2), a reference electrode (3), an inlet (4) of a buffering solution and an enzyme solution, a liquid outlet (5), an inlet (6) of the buffering solution and liquid to be detected, a reaction tank (7), a magnetic stirring rotor (8), an electrode fixing body (9), an external structural body (10), a quantitative enzyme solution injecting device (11), and a quantitative injecting device (14) of the liquid to be detected. The enzyme solution and the liquid to be detected are directly injected into the reaction tank in a quantitative injection manner, thereby improving the detecting speed and accuracy of the sensor; the isolated magnetic stirring rotor is arranged in the reaction tank to ensure faster and more complete electrochemical reaction of the reactants so as to improve the detecting sensitivity; and the glucose biosensor is conveniently combined an FIA flow injection analysis system to simultaneously perform online high pressure vapor sterilization on the sensor together with a fermentation tank and easily realize online detection of glucose.
Description
Technical field
The present invention relates to the glucose detection technology of biology sensor formula, it is the biology sensor for detection of glucose of a kind of comprehensive employing physics, chemistry, biology, electronics, specifically, be a kind of glucose biological sensor that is applicable to online detection towards sweat, belong to field of biosensors.
Background technology
1962, Clark and Lyons proposed the concept of " enzyme electrode Enzyme electrode " for the first time, and this event with milestone significance is considered to the initial of biology sensor development, and therefore Clark also obtains the title of " father of biology sensor ".Updike in 1967 and Hicks have successfully made first glucose enzyme electrode biology sensor according to this principle.
The principle of glucose enzyme electrode biology sensor is an electrochemical reaction process, be that example is that electrochemical reaction takes place in reaction tank for glucose oxidase and glucose with the glucose oxidase, the electronics that generates, form electric current, obtain current signal or voltage signal by testing circuit by electrode, thereby realize the detection of glucose.According to the difference of used electron transfer mediator, the glucose enzyme electrode biology sensor can be divided into the three generations.First generation biology sensor with oxygen molecule or hydrogen peroxide as electronics transfer medium between enzyme and electrode, the proportional relation of the size of current of generation and oxygen concentration.The second generation adopts artificial electron transfer mediator such as the potassium ferricyanide/potassium ferrocyanide, ferrocene and derivant thereof, dye molecule, organic conductive salt, metal phthalocyanine etc. as the electronics transfer medium, and the artificial redox reaction of electronics transfer medium on electrode is reversible.The third generation does not need electron transfer mediator, and the electronics transmission directly takes place between electrode and enzyme.
In the sweat, in order to prevent microbiological contamination, online glucose biological sensor and and reactor between pipeline must be able to bear the high-temperature steam sterilization, descend or forfeiture to avoid its performance of sterilization back, so glucose biological sensor mainly is off-line type at present, online is also at the experimental stage.Yet glucose is reactant and intermediate product common in the fermentation process, and the control to concentration of glucose in the sweat can be directly connected to product productive rate and quality.Thereby the detection of the real-time online of concentration of glucose is very important and necessary.Therefore, extremely need now a kind of for being applicable to of sweat online, fast, glucose biological sensor reliable, easy to use.
Summary of the invention
The invention provides a kind of glucose biological sensor that is applicable to online detection towards sweat.
This glucose biological sensor, it is characterized in that: this sensor comprises: working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid entrance 4, liquid outlet 5, damping fluid and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring rotator 8, electrode fixed body 9, external structure body 10, enzyme liquid calibrated shot device 11, liquid calibrated shot device 14 to be measured, and concrete annexation is:
Described working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 9; Electrode fixed body 9 is fixed in the external structure body 10, and external structure body 10 constitutes reaction tank 7 with electrode fixed body 9; Be built-in with magnetic stirring rotator 8 in the reaction tank 7;
Have damping fluid and enzyme liquid entrance 4, damping fluid and liquid entrance 6 to be measured, liquid outlet 5 on the electrode fixed body 9; Described damping fluid is connected with the damping fluid bottle with enzyme liquid calibrated shot device 11 with enzyme liquid entrance 4; Described damping fluid is connected with the damping fluid bottle with liquid calibrated shot device 14 to be measured with liquid entrance 6 to be measured; Described liquid outlet 5 is connected with waste liquid bottle;
Working electrode 1 is shaped as cylindrical or annular, is made by metal material or conductive material.
Be shaped as cylindrical or annular to electrode 2, made by metal material or conductive material.
Electrode fixed body 9 is shaped as cylindrical that the bottom is cut, and is made by high temperature resistant nonmetallic materials.
Described glucose biological sensor adopts enzyme liquid and liquid calibrated shot mode to be measured.
Advantage of the present invention is:
Enzyme exists in the mode of enzyme liquid, namely is dissolved in damping fluid by enzyme and adjuvant and makes, and is simple to operate;
Enzyme exists in the enzyme liquid mode of " fresh ", and is independent with liquid to be measured, is not subjected to ectocine, makes sensor not need frequent calibration;
Change different types of enzyme liquid, can detect different material, namely a sensor can detect multiple material;
Liquid to be measured is obtained by add screen pack before sampling pipe, saves liquid dialysis part to be measured in the sensor, to simplify sensor construction;
The detection cost is low, about 0.01 yuan of the used enzyme liquid of single cost;
Adopt the calibrated shot mode directly with enzyme liquid and liquid calibrated shot to be measured in reaction tank, improved detection speed and the degree of accuracy of sensor;
The built-in isolated electromagnetic agitation rotor of reaction tank makes the reactant electrochemical reaction more fast fully, and then has improved detection sensitivity;
Conveniently be combined with FIA Flow Injection Analysis system, can realize that sensor carries out online high pressure steam sterilization simultaneously with fermentation tank, realizes the online detection of glucose easily;
Invent simple and compact for structurely, easy to process, be easy to mass production.
Description of drawings
Fig. 1 is the agent structure synoptic diagram that is used for the glucose biological sensor of online detection.
Fig. 2 is the structural representation of enzyme liquid calibrated shot device in this glucose biological sensor.
Fig. 3 is the structural representation of liquid calibrated shot device to be measured in this glucose biological sensor.
Among the figure: 1, working electrode, 2, to electrode, 3, contrast electrode, 4, damping fluid and enzyme liquid entrance, 5, liquid outlet, 6, damping fluid and liquid entrance to be measured, 7, reaction tank, 8, the magnetic stirring rotator, 9, electrode fixed body, 10, external structure body, 11, enzyme liquid calibrated shot device, 12, the electromagnetism six-way valve
I, 13, quantitative ring
I, 14, liquid liquid calibrated shot device to be measured, 15, the electromagnetism six-way valve
II, 16, quantitative ring
II
Embodiment
As Fig. 1, Fig. 2 and shown in Figure 3, structure of the present invention is by working electrode 1, to electrode 2, and contrast electrode 3, damping fluid and enzyme liquid entrance 4, liquid outlet 5, damping fluid and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring rotator 8, electrode fixed body 9, external structure body 10.
The glucose biological sensor that is used for online detection, it is characterized in that: this sensor comprises: working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid entrance 4, liquid outlet 5, damping fluid and liquid entrance 6 to be measured, reaction tank 7, magnetic stirring rotator 8, electrode fixed body 9, external structure body 10, enzyme liquid calibrated shot device 11, liquid calibrated shot device 14 to be measured, and concrete annexation is:
Described working electrode 1, electrode 2 and contrast electrode 3 usefulness epoxy resin are fixed in three electrode holes on the electrode fixed body 9; Electrode fixed body 9 is fixed in the external structure body 10 by the spiral card cap, and external structure body 10 constitutes reaction tank 7 with electrode fixed body 9; Be built-in with magnetic stirring rotator 8 in the reaction tank 7;
Have damping fluid and enzyme liquid entrance 4, damping fluid and liquid entrance 6 to be measured, liquid outlet 5 on the electrode fixed body 9; Described damping fluid is connected with the damping fluid bottle with enzyme liquid calibrated shot device 11 with enzyme liquid entrance 4; Described damping fluid is connected with the damping fluid bottle with liquid calibrated shot device 14 to be measured with liquid entrance 6 to be measured; Described liquid outlet 5 is connected with waste liquid bottle;
Enzyme liquid calibrated shot device 11 is by the electromagnetism six-way valve
I12 and quantitative ring
I13 constitute, quantitatively ring
I13 two ends are connected in the electromagnetism six-way valve
I12 any diagonal angle mouths.The electromagnetism six-way valve
I12 wherein a bites are connected to damping fluid and enzyme liquid entrance 4.
Liquid calibrated shot device to be measured 14 is by the electromagnetism six-way valve
II15 and quantitative ring
II16 constitute, quantitatively ring
II16 two ends are connected in the electromagnetism six-way valve
II15 any diagonal angle mouths.The electromagnetism six-way valve
II15 wherein a bites are connected to damping fluid and enzyme liquid entrance
II6.
Wherein, described working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 9, have constituted the electrochemical measurement three-electrode system jointly.
Working electrode 1 is shaped as cylindrical, but is not limited to cylindrically, and can be such as but not limited to by metal material or conductive material: platinum, gold, silver, graphite etc. are made.
Electrode 2 is shaped as cylindrical, but is not limited to cylindrically, can be such as but not limited to by metal material or conductive material: platinum, gold, silver, graphite etc. are made.
Electrode fixed body 9 is shaped as cylindrical that the bottom is cut, and can be such as but not limited to by high temperature resistant nonmetallic materials: polyetheretherketone PEEK, polysulfones etc. are made.
Before the sensor measurement sample, with buffer solution for cleaning reaction tank 7; Then, liquid to be measured is introduced into reaction tank 7 by liquid calibrated shot device to be measured, starts the magnetic stirring rotator; Then, by enzyme liquid calibrated shot device glucose oxidase liquid is expelled in the reaction tank 7, glucose oxidase liquid and glucose react under the three-electrode system effect in reaction tank 7, produce current signal, drawn by working electrode 1, this current signal is by the I/V translation circuit in the three electrode detection circuit, become voltage signal, then to voltage signal amplification, filtering, obtain one and the proportional voltage signal of concentration of glucose to be measured, this voltage signal amplitude namely represents liquid concentration of glucose to be measured.
Claims (7)
1. the glucose biological sensor that is used for online detection, it is characterized in that: this sensor comprises: working electrode (1), to electrode (2), contrast electrode (3), damping fluid and enzyme liquid entrance (4), liquid outlet (5), damping fluid and liquid entrance to be measured (6), reaction tank (7), magnetic stirring rotator (8), electrode fixed body (9), external structure body (10), enzyme liquid calibrated shot device (11), liquid calibrated shot device to be measured (14), and concrete annexation is:
Described working electrode (1), electrode (2) and contrast electrode (3) are fixed in the electrode fixed body (9); Electrode fixed body (9) is fixed in the external structure body (10), and external structure body (10) constitutes reaction tank (7) with electrode fixed body (9); Be built-in with magnetic stirring rotator (8) in the reaction tank (7);
Have damping fluid and enzyme liquid entrance (4), damping fluid and liquid entrance to be measured (6), liquid outlet (5) on the electrode fixed body (9); Described damping fluid is connected with the damping fluid bottle with enzyme liquid calibrated shot device (11) with enzyme liquid entrance (4); Described damping fluid is connected with the damping fluid bottle with liquid calibrated shot device to be measured (14) with liquid entrance to be measured (6); Described liquid outlet (5) is connected with waste liquid bottle.
2. glucose biological sensor according to claim 1, it is characterized in that: electrode fixed body (9) is shaped as cylindrical that the bottom is cut, and is made by the corrosion-and high-temp-resistant nonmetallic materials; Described high temperature resistant nonmetallic materials comprise polyetheretherketone PEEK, polysulfones.
3. glucose biological sensor according to claim 1, it is characterized in that: external structure body (10) is made by high temperature resistant nonmetallic materials; Described corrosion-and high-temp-resistant nonmetallic materials comprise polyetheretherketone PEEK, polysulfones.
4. glucose biological sensor according to claim 1, it is characterized in that: reaction tank is made of external structure body and electrode fixed body, the reaction tank volume is that 400 μ L are to 1000 μ L, and magnetic stirring rotator body places reaction tank, volume is 200 μ L～500 μ L, and is no more than 50% of reaction tank volume.
5. glucose biological sensor according to claim 1 is characterized in that: magnetic stirring rotator (8) is made by the corrosion-and high-temp-resistant nonmetallic materials of band magnetic core; Magnet of the same race fixing on the outer micro DC machine rotor of magnet in the magnetic stirring rotator (8) and sensor is parallel, and magnetic stirring rotator (8) rotates and rotates along with motor, realizes that with this noncontact stirs.
6. glucose biological sensor according to claim 1 is characterized in that: adopt the calibrated shot mode directly from damping fluid and enzyme liquid entrance (4) and damping fluid and liquid entrance to be measured (6) with enzyme liquid and liquid calibrated shot to be measured to reaction tank; Wherein enzyme liquid is dissolved in damping fluid by enzyme and adjuvant and makes, and liquid to be measured adds screen pack and obtains before sampling pipe.
7. glucose biological sensor according to claim 1 is characterized in that: described working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 9, have constituted the electrochemical measurement three-electrode system jointly;
Working electrode 1 is shaped as cylindrical, comprises that by conductive material platinum, gold, silver, graphite makes;
Electrode 2 is shaped as cylindrical, comprises that by conductive material platinum, gold, silver, graphite makes;
Contrast electrode 3 is shaped as cylindrical, is made of Ag/AgCl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310144353.4A CN103235016B (en) | 2013-04-23 | 2013-04-23 | A kind of glucose biological sensor for on-line checking |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310144353.4A CN103235016B (en) | 2013-04-23 | 2013-04-23 | A kind of glucose biological sensor for on-line checking |
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| CN103235016A true CN103235016A (en) | 2013-08-07 |
| CN103235016B CN103235016B (en) | 2016-12-28 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104777192A (en) * | 2015-03-18 | 2015-07-15 | 浙江工商大学 | Detection method of food sweetness degree |
| CN105319260A (en) * | 2015-11-05 | 2016-02-10 | 北京农业信息技术研究中心 | On-line plant glucose detection method and device based on microelectrode biosensing technology |
| CN107202830A (en) * | 2017-06-15 | 2017-09-26 | 南京工业大学 | A kind of device detected based on prussian blue film bioelectrode |
| CN107271514A (en) * | 2017-06-15 | 2017-10-20 | 南京工业大学 | It is a kind of based on detection method of the prussian blue film bioelectrode to glucose |
| CN108896340A (en) * | 2018-03-23 | 2018-11-27 | 吉林农业大学 | A kind of multiple pipe drilling core bit for soil drill sampling |
| CN109342734A (en) * | 2018-12-20 | 2019-02-15 | 中山大学附属第八医院（深圳福田） | A kind of portable blood sugar detector |
| CN110523355A (en) * | 2018-05-23 | 2019-12-03 | 青岛千和电子有限公司 | Oxidation reaction generator |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104777192A (en) * | 2015-03-18 | 2015-07-15 | 浙江工商大学 | Detection method of food sweetness degree |
| CN104777192B (en) * | 2015-03-18 | 2017-06-20 | 浙江工商大学 | A kind of detection method of food sugariness |
| CN105319260A (en) * | 2015-11-05 | 2016-02-10 | 北京农业信息技术研究中心 | On-line plant glucose detection method and device based on microelectrode biosensing technology |
| CN105319260B (en) * | 2015-11-05 | 2017-10-31 | 北京农业信息技术研究中心 | The online glucose sensing approach of plant and device based on microelectrode biosensing technology |
| CN107202830A (en) * | 2017-06-15 | 2017-09-26 | 南京工业大学 | A kind of device detected based on prussian blue film bioelectrode |
| CN107271514A (en) * | 2017-06-15 | 2017-10-20 | 南京工业大学 | It is a kind of based on detection method of the prussian blue film bioelectrode to glucose |
| CN108896340A (en) * | 2018-03-23 | 2018-11-27 | 吉林农业大学 | A kind of multiple pipe drilling core bit for soil drill sampling |
| CN110523355A (en) * | 2018-05-23 | 2019-12-03 | 青岛千和电子有限公司 | Oxidation reaction generator |
| CN109342734A (en) * | 2018-12-20 | 2019-02-15 | 中山大学附属第八医院（深圳福田） | A kind of portable blood sugar detector |
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| Publication number | Publication date |
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| CN103235016B (en) | 2016-12-28 |
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