A kind of immune labeled slide turntable
Technical field
The utility model is related to immune labeled equipment technical field, specially a kind of immune labeled slide turntable.
Background technology
It is immune labeled, it is applied immunology basic principle --- antigen-antibody reaction, i.e. antigen is combined with antibody specificity
Principle, so that the color developing agent (fluorescein, enzyme, metal ion, isotope) of labelled antibody is developed the color by chemical reaction and determine group
Intracellular antigen (peptide and protein) is knitted, it is positioned, qualitative and relative quantification research, referred to as immunohistochemistry
Technology or immunocytochemical technique.
During carrying out immune labeled experiment, can generally use slide turntable to be placed with histiocytic slide into
Row is fixed, but the slide turntable for being conventionally used to immunization experiment wastes reagent, traditional slide turntable when carrying out dyeing processing
The reagent consumed in tiling needs excessive could realize to be completely spread out experimental cell, is unfavorable for immune labeled better
It carries out.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides a kind of immune labeled slide turntable, solves traditional use
In immunization experiment slide turntable carry out dyeing processing when waste reagent the problem of.
(2) technical solution
In order to achieve the above object, the utility model is achieved by the following technical programs:A kind of immune labeled slide turn
Disk, including slide turntable body, offer connector at the center of the slide turntable body, in the slide turntable body and
It is equidistant around multigroup mounting hole is offered by the center of circle of connector, the position etc. of mounting hole is corresponded in the slide turntable body
Rang ring is placed with slide ontology around slide placing groove is offered on the slide placing groove, in the slide turntable body simultaneously
And the position of corresponding mounting hole is bolted and is equipped with fixed seat, is connected with by axis in the fixed seat and glass
The compatible glass frame of piece ontology.
Preferably, the glass frame includes slide lid and pressing handle, and pressing handle is corresponded in the slide turntable body
Position offer card slot, and press on handle integrally formed with wedge-shaped fixture block compatible with card slot.
Preferably, the side of the slide lid far from slide turntable body offers reagent and slot and waste liquid port is added, described
The slot bottom that slot is added in reagent is oblique, and slot is added close to fixed seat in reagent.
Preferably, the slide lid offers reagent trough and groove close to the side of slide ontology, and reagent trough is close to solid
The inside of reservation, the reagent trough is equipped with sealing rubber ring, and the waste liquid port is connected to reagent trough.
(3) advantageous effect
The utility model provides a kind of immune labeled slide turntable.Has following advantageous effect:
1, the immune labeled slide turntable ensure that the glass when carrying out dyeing processing by the special setting to glass frame
Reagent trough and slide ontology fit closely on horse, since the principle that surface tension of liquid and capillary are creeped promotes reagent
Stable slave reagent is added slot and is flowed into slide body surface, ensure that and rests on reagent that reagent is added in slot gradually to waste liquid
Mouth is mobile, realizes the effect for carrying out being completely spread out dyeing to the experimental cell on slide ontology, reduces immune labeled reaction
The reagent consumed.
2, the immune labeled slide turntable, by middle shaft rotation between glass frame and slide turntable body, and passes through wedge shape
Fixture block and card slot are fastened, and prevent influence of the extraneous factor to experimental result, the opening closed manners and structure of glass frame are more
Hommization, fastens and opening operation is simple and quick, does experiment enhanced convenience, considerably increases the feasibility of experiment.
Description of the drawings
FIG. 1 is a schematic structural view of the utility model;
Fig. 2 is the structural schematic diagram of the utility model slide turntable body;
Fig. 3 is the partial structurtes enlarged drawing in the portions A in the utility model Fig. 1;
Fig. 4 is the structural schematic diagram that the utility model glass frame is faced;
Fig. 5 is the structural schematic diagram that the utility model glass frame is looked up;
Fig. 6 is the structural schematic diagram of the utility model fixed seat;
Fig. 7 is the structural schematic diagram of the utility model sealing rubber ring.
In figure:1 slide turntable body, 2 connectors, 3 mounting holes, 4 slide placing grooves, 5 slide ontologies, 6 bolts, 7 are fixed
Slot, 912 waste liquid ports, 913 reagents are added in seat, 71 through-holes, 72 first pin holes, 8 axis, 9 glass frames, 91 slide lids, 911 reagents
Slot, 914 grooves, 915 sealing rubber rings, 92 pressing handles, 93 wedge-shaped fixture blocks, 94 notches, 10 card slots, 11 second pin holes.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
The utility model embodiment provides a kind of immune labeled slide turntable, as shown in Figs. 1-3, including slide turntable body
1, connector 2 is offered at the center of slide turntable body 1, for the equidistant ring in the center of circle in slide turntable body 1 and with connector 2
Around multigroup mounting hole 3 is offered, equidistant surround in position that mounting hole 3 is corresponded in slide turntable body 1 offers slide placement
Slot 4, is placed with slide ontology 5 on slide placing groove 4, and in the slide turntable body 1 and position of corresponding mounting hole 3 passes through bolt
6 are installed with fixed seat 7, and glass frame 9 compatible with slide ontology 5 is connected with by axis 8 in fixed seat 7, when
When needing to carry out immune labeled operation, the reversion of glass frame 9 is pressed onto using axis 8 and is placed in advance in slide placing groove 4
Slide ontology 5 on, the hinged cooperation of glass frame 9 and fixed seat 7, which reaches, can be directly realized by when placing slide ontology 5 to glass
The pressing of piece ontology 5, the realization of sealing rubber ring 915 being mounted in reagent trough 913 using pressing are consolidated to what is reacted on slide ontology 5
Determine region and carries out edge seal;
Wherein, connector 2 is by a connecting shaft and the centrifugation on the experimental provision ontology of placement slide turntable body 1
Rotating mechanism connection, and the reporting in 174r/min or more of rotating mechanism is centrifuged, pass through the reciprocating rotary glass for adjusting connecting shaft
Piece turntable body 1 enters 5 top of slide ontology in slide turntable body 1 to be conducive to reagent, and is also beneficial to
Reach the effect promoting reaction rate that concussion shakes up through entering the reagent on slide ontology 5, it is further seldom in reagent dosage
In the case of promote to realize and carry out the effect of corresponding reagent tiling to the histocyte to tile on slide ontology 5, greatly save
The dosage of reagent, while making the cost of experiment lower, reaction is also more fully.
- 5 are please referred to Fig.1, glass frame 9 includes slide lid 91 and pressing handle 92, is corresponded in slide turntable body 1 and presses handle
The position of handle 92 offers card slot 10, and presses on handle 92 integrally formed with wedge-shaped fixture block 93 compatible with card slot 10, glass
Side of the piece lid 91 far from slide turntable body 1 offers reagent and slot 911 and waste liquid port 912 is added, and the slot of slot 911 is added in reagent
Bottom is oblique, and slot 911 is added close to fixed seat 7 in reagent, and slide lid 91 offers reagent trough close to the side of slide ontology 5
913 and groove 914, reagent trough 913 uses inclined design, and reagent trough 913, close to fixed seat 7, the inside of reagent trough 913 is installed
There is sealing rubber ring 915, sealing rubber ring 915, which plays, prevents from entering what the reagent inside reagent trough 913 was oozed out from reagent trough 913
Effect so that experiment preferably carries out, and waste liquid port 912 is connected to reagent trough 913, sealing rubber ring 915, glass frame 9 and slide sheet
The space that body 5 is constituted is the section that reagent flows into, and when needing that reagent is added, reagent is instilled the inside that slot 911 is added in reagent,
The reagent that reagent is added inside slot 911 can be flowed into reagent trough 913, and due to the inclined design of reagent trough 913, reagent is being flowed into
It can be flowed away to reagent trough 913 far from 7 side of fixed seat when reagent trough 913, rest on the reagent that reagent is added in slot 911 because of liquid
It on the principle infiltration leakage to slide ontology 5 that body surface tension and capillary are creeped, and is moved to waste liquid port 912, to reach
To needing to carry out the effective tiling dyeing of immune labeled cell progress on slide ontology 5;
Wherein, pass through the cooperation of wedge-shaped fixture block 93 and card slot 10 on pressing handle 92 so that slide ontology 5 is placed on slide
Enhanced convenience when taking inside placing groove 4 or from the inside of slide placing groove 4, and glass frame 9 and slide turntable body 1
It fastens so that whole without folding in experiment, reduce glass frame 9 prevents extraneous factor to experimental result with extraneous contact
It influences, and the opening closed manners of glass frame 9 are more humane with structure, do experiment and carry out enhanced convenience, considerably increase reality
The feasibility tested;
By the special setting to glass frame 9, ensure that carry out dyeing processing when glass frame 9 on reagent trough 913 and glass
Piece ontology 5 fits closely, and promotes the slave reagent of stable reagent that slot 911 is added and is flowed into 5 surface of slide ontology, realizes to glass
Experimental cell on piece ontology 5 carries out the effect for being completely spread out dyeing, reduces dyeing and handles consumed reagent.
Please refer to Fig.1-6, offer 6 compatible through-hole 71 of bolt in fixed seat 7, the both sides of fixed seat 7 offer with
8 compatible first pin hole 72 of axis, the side of the close fixed seat 7 of slide lid 91 open up jagged 94, are opened up on notch 94
There is second pin hole 11 compatible with axis 8, and axis 8 passes through the first pin hole 72 and the second pin hole 11, at the end of experiment, to
Cleaning solution is added in the inside that slot 911 is added in reagent, since at this moment reagent will flow into waste liquid port for the inclined design of reagent trough 913
Inside 912, under the connecting shaft rotation being connect with centrifugation rotating mechanism on experimental provision ontology, promotes reagent to be centrifuged out slide and turn
Disk ontology 1 thoroughly cleans remaining reagent on slide ontology 5 to realize.
Operation principle:When carrying out reagent to the histocyte on slide ontology 5, reagent is added to reagent first and is added
The inside of slot 911, liquid can flow into the inside of reagent trough 913, because reagent trough 913 be designed as inclined-plane thus reagent can be flowed into examination
Agent slot 913 is paved close to the side of fixed seat 7 and entirely needs pigmented section, and after the reaction time arrives, cleaning solution is injected into
The inside of slot 911 is added in reagent, and cleaning solution can be flowed into reagent trough 913 close to the side of fixed seat 7, and originally in pigmented section
Reagent can be then discharged by waste liquid port 912, excess cleaning fluid, which need to be added, in this step ensures pigmented section noresidue reagent, has cleaned
Cleaning solution is only existed at post-reaction zone, and slide turntable body 1 with centrifugation rotating mechanism by connecting on experimental provision ontology
Connecting shaft high speed rotation makes slide turntable body 1 be rotated, while during the reaction to slide turntable body 1 into line tilt
It shakes up, cleaning solution can then be flowed out from waste liquid port 912, as in next step or next time anti-to realize emptying operation to turntable
Reaction compartment should be stayed, experiment when to realize that histocyte carries out immune labeled.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.