CN207976390U - A kind of medical unicellular inner wall detection device - Google Patents
A kind of medical unicellular inner wall detection device Download PDFInfo
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- CN207976390U CN207976390U CN201820370642.4U CN201820370642U CN207976390U CN 207976390 U CN207976390 U CN 207976390U CN 201820370642 U CN201820370642 U CN 201820370642U CN 207976390 U CN207976390 U CN 207976390U
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- 238000012360 testing method Methods 0.000 claims abstract description 36
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Abstract
The utility model provides a kind of medical unicellular inner wall detection device, the device captures cell by optical fiber optical tweezers, forms three pairs of detection faces using six core fibres, realizes the detection to single celled inner wall, the problems such as detection is not easy, and testing result accuracy is insufficient, is solved inside unicellular.The device includes light source, light source tail optical fiber, fiber optic splitter, optical coupling lens group, six core fibres, strong trapping stiffness optical fiber optical tweezers, the micro- behaviour's hand I of three-D electric, the micro- behaviour's hand II of three-D electric, laser light source, spectrometer and microscope；The light of light source passes through in light source tail optical fiber input optical fibre beam splitter, the output light of fiber optic splitter is coupled by optical coupling lens group in three fibre cores of six core fibres, the output light of the other three fibre core of six core fibres is coupled into three root receiving fibers and incoming spectrometer by optical coupling lens group, the test side of six core fibres is connect with the control terminal of the micro- behaviour's hand I of three-D electric, and the output end of strong trapping stiffness optical fiber optical tweezers is connect with the control terminal of the micro- behaviour's hand II of three-D electric.
Description
Technical field
The utility model is related to medical sciences, are in particular a kind of medical unicellular inner wall detection devices.
Background technology
Cell Measurement Technique has developed some time, but can all occur it in annual Nature Methods prospect technologies
Figure, this is mainly due to the cells in culture medium or body there are diversity, or perhaps heterogeneous, this is many realities
It tests analysis and causes obstacle, therefore with the development of modern biology, " average value " this word cannot meet the need of people
It has been wanted that, people are it is to be understood that otherness between cell.If various unicellular collection of illustrative plates can be integrated, researcher can be helped
Understand gene regulation and the heterogeneous mechanism of action of gene, small due to cell, detection is more difficult, detection it is accurately relatively low, because
This cell detection device extremely has research necessary.Optical fiber has obtained answering extensively in biomedical, sensing, communication and the fields such as detection
There are many merits as transmission medium with, optical fiber, such as electrical insulating property is good, chemical property is stable, transmission speed is fast, and can be
It works under the rugged environments such as inflammable and explosive, toxic, high temperature and pressure, with wide Research Prospects.Optical fiber surface plasmon
Resonance body (Surface Plasmon Resonance, SPR) sensor be mainly to the variation of a certain physical quantity of external environment into
Row perception and transmission, physical quantity can be temperature, liquid refractivity, pressure etc..
It is more and more deep to the research of cell to people with the development and progress of society, cell is carried out by optical fiber
Detection also buds out into popularity, and unicellular is micron dimension, suitable with the end face magnitude of optical fiber, is generally to carry out cell to cell detection
Slice, or the detection of characteristic is carried out in outside, in order to further be familiar with cell characteristics, the utility model provides one
The medical unicellular inner wall detection device of kind, the device capture cell by optical fiber optical tweezers, utilize six of six core fibres
Fibre core forms three pairs of detection faces, realizes the detection to single celled inner wall, can realize unicellular interior multidirectional detection so that
The problems such as testing result is more acurrate, solves unicellular internal detection and is not easy, and testing result accuracy is insufficient.
Invention content
The utility model provides a kind of medical unicellular inner wall detection device, the device by optical fiber optical tweezers to cell into
Row capture forms three pairs of detection faces using six fibre cores of six core fibres, realizes the detection to single celled inner wall, can realize
Unicellular interior multidirectional detection so that testing result is more acurrate, solves unicellular internal detection and is not easy, testing result is accurate
The problems such as property is insufficient.
To achieve the above object, the utility model provides a kind of medical unicellular inner wall detection device, this is novel slender
The rotatable multi-direction detection device of born of the same parents includes light source, light source tail optical fiber, fiber optic splitter, optical coupling lens group, six core fibres, Qiang Guang
The micro- behaviour's hand I of trap power optical fiber optical tweezers, three-D electric, the micro- behaviour's hand II of three-D electric, laser light source, spectrometer and microscope；
Light source is the super continuum source of spectral width 450nm -1100nm, the excitation for generating excitation SPR phenomenons
Light；Light source tail optical fiber is used for transmission the light of light source；Fiber optic splitter is used to the light of light source being divided into three beams；The spectral width of spectrometer
For 450nm -1100nm, for receiving, preserving and showing that SPR sensorgram spectrum, the input terminal of spectrometer are provided with spectrometer reception
Optical fiber；Six core fibres for detecting detection cell, the test sides of six core fibres be provided with SPR detection faces I, SPR detection faces II,
SPR detection faces III, reflecting surface I, reflecting surface II and reflecting surface III, it is SPR detection faces I, SPR detection faces II, SPR detection faces III, anti-
It includes a fibre core to penetrate face I, reflecting surface II and reflecting surface III respectively, and SPR detection faces I and reflecting surface I are opposite, II He of SPR detection faces
Reflecting surface II is opposite, and SPR detection faces III and reflecting surface III are opposite；The three beams that optical coupling lens group is used to separate fiber optic splitter
The output light of the other three fibre core of six core fibres is coupled into three spectrometers into three fibre cores of six core fibres by optical coupling
In reception optical fiber；Strong trapping stiffness optical fiber optical tweezers are used for Acquisition Detection cell；The micro- behaviour's hand I of three-D electric is for adjusting six core fibres
Position, the micro- behaviour's hand II of three-D electric are used to adjust the position of strong trapping stiffness optical fiber optical tweezers；Laser light source is for generating Acquisition Detection
The output end of the light of cell, laser light source is provided with light source output optical fiber；Microscope be used for determine six core fibres test side and
The position of the output end of strong trapping stiffness optical fiber optical tweezers；
The output end of light source is connect with the input terminal of light source tail optical fiber, the input of the output end and fiber optic splitter of light source tail optical fiber
End connection, the output end of fiber optic splitter couple the light into three fibres at the non-detection end of six core fibres by optical coupling lens group
In core, the output light of the other three fibre core of six core fibres is coupled into three root receiving fibers and incoming spectrometer by optical coupling lens group
In, the test side of six core fibres is connect with the control terminal of the micro- behaviour's hand I of three-D electric, the output end and three of strong trapping stiffness optical fiber optical tweezers
Tie up the control terminal connection of electronic micro- behaviour's hand II, the input terminal of strong trapping stiffness optical fiber optical tweezers and the output end face of light source output optical fiber
Welding；The capture inspection of the test side of six core fibres, the output end of strong trapping stiffness optical fiber optical tweezers and the capture of strong trapping stiffness optical fiber optical tweezers
Cell is surveyed to be placed in the microscopical eyepiece visual field.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Light source tail optical fiber be single mode optical fiber or less fundamental mode optical fibre.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
SPR detection faces I, SPR detection faces II and SPR detection faces III surface be coated with the nano-gold film of 50nm respectively.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Reflecting surface I, reflecting surface II and reflecting surface III surface be coated with the nano-gold film of 300nm respectively.
As advanced optimizing for the utility model, a kind of novel unicellular inner wall detection device of the utility model is described
Six fibre cores of six core fibres be distributed on a concentric circles and symmetrical two-by-two.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Strong trapping stiffness optical fiber optical tweezers be optical fibre ring optical tweezer.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Laser light source be 532nm laser light source or 980nm laser light source.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Light source output optical fiber be single mode optical fiber or less fundamental mode optical fibre.
As advanced optimizing for the utility model, a kind of medical unicellular inner wall detection device of the utility model is described
Spectrometer reception optical fiber be step multimode fibre or gradual change multimode fibre.
A kind of medical unicellular inner wall detection device of the utility model has the beneficial effect that：
1. the utility model provides a kind of medical unicellular inner wall detection device, the device is by optical fiber optical tweezers to cell
It is captured, forms three pairs of detection faces using six fibre cores of six core fibres, realize the detection to single celled inner wall, Neng Goushi
Existing unicellular interior multidirectional detection so that testing result is more acurrate, solves unicellular internal detection and is not easy, testing result is accurate
The problems such as really property is insufficient.
2. the utility model captures cell by optical fiber optical tweezers, three pairs are formed using six fibre cores of six core fibres
Detection faces realize the detection to single celled inner wall, can realize unicellular interior multidirectional detection, the accuracy rate of testing result
It is high.
3. the utility model uses optical fiber sensing principle, very sensitively single celled characteristic can be sensed,
It is strong to single celled characteristic variations sensing capability, there is higher detection sensitivity.
4. the utility model transmits testing result by optical fiber, detection speed is fast, and detection efficiency is high.
5. a kind of medical unicellular inner wall detection device of the utility model is simple for structure, device materials are easily sought, device knot
Structure is simple, easy and convenient to handle.
Description of the drawings
The utility model is described in more detail with specific implementation method below in conjunction with the accompanying drawings.
Fig. 1 is a kind of structural schematic diagram of medical unicellular inner wall detection device of the utility model；
Fig. 2 is a kind of unicellular capture of medical unicellular inner wall detection device of the utility model and the schematic diagram of detection；
Fig. 3 is the enlarged diagram of the unicellular capture of the utility model；
Fig. 4 is the enlarged diagram of the utility model Single cell analysis；
Fig. 5 is the schematic diagram of the optical coupling of the utility model optical coupling lens group；
Fig. 6 is the schematic diagram that six core fibre of the utility model detects end-view；
Fig. 7 is the schematic diagram of the front view of six core fibre test side of the utility model.
In figure：Light source 1；Light source tail optical fiber 2；Fiber optic splitter 3；Optical coupling lens group 4；Six core fibres 5；I 5- of SPR detection faces
1；II 5-2 of SPR detection faces；III 5-3 of SPR detection faces；I 5-4 of reflecting surface；II 5-5 of reflecting surface；III 5-6 of reflecting surface；Strong trapping stiffness light
Fine optical tweezer 6；The micro- behaviour's hand of three-D electric I 7；The micro- behaviour's hand of three-D electric II 8；Optical fiber bonding machine 9；Laser light source 10；Light source output light
Fine 10-1；Spectrometer 11；Spectrometer reception optical fiber 11-1；Detect cell 12；Microscope 13.
Specific implementation mode
Illustrate that present embodiment, the utility model provide a kind of medical unicellular with reference to Fig. 1,2,3,4,5,6,7
Inner wall detection device, the device capture cell by optical fiber optical tweezers, and three pairs are formed using six fibre cores of six core fibres
Detection faces realize the detection to single celled inner wall, can realize unicellular interior multidirectional detection so that testing result is more accurate
Really, the problems such as detection is not easy, and testing result accuracy is insufficient, is solved inside unicellular.
To achieve the above object, the utility model provides a kind of medical unicellular inner wall detection device, this is novel slender
The rotatable multi-direction detection device of born of the same parents includes light source 1, light source tail optical fiber 2, fiber optic splitter 3, optical coupling lens group 4, six core fibres
5, the micro- behaviour's hand I 7 of strong trapping stiffness optical fiber optical tweezers 6, three-D electric, the micro- behaviour's hand II 8 of three-D electric, laser light source 10,11 and of spectrometer
Microscope 13；
Light source 1 is the super continuum source of spectral width 450nm -1100nm, the excitation for generating excitation SPR phenomenons
Light, light source 1 are the super continuum source of U.S. Energetiq companies LDLS series EQ-99FC；Light source tail optical fiber 2 is used for transmission light
The light in source 1；Fiber optic splitter 3 is used to the light of light source 1 being divided into three beams；The spectral width of spectrometer 11 is 450nm -1100nm,
For receiving, preserving and showing that SPR sensorgram spectrum, the input terminal of spectrometer 11 are provided with spectrometer reception optical fiber 11-1, spectrum
Instrument 11 is the AQ6370C spectrometers of Japanese Yokogawa；For detecting detection cell 12, the test side of six core fibres 5 is set six core fibres 5
It is equipped with I 5-1, SPR detection faces of SPR detection faces, II 5-2, SPR detection faces, III 5-3, I 5-4 of reflecting surface, II 5-5 of reflecting surface and reflection
III 5-6 of face, I 5-1, SPR detection faces of SPR detection faces, II 5-2, SPR detection faces, III 5-3, I 5-4 of reflecting surface, II 5-5 of reflecting surface and
III 5-6 of reflecting surface includes respectively a fibre core, and SPR detection faces I 5-1 and I 5-4 of reflecting surface is opposite, II 5-2 of SPR detection faces and reflection
II 5-5 of face is opposite, and SPR detection faces III 5-3 and III 5-6 of reflecting surface is opposite；Optical coupling lens group 4 is for dividing fiber optic splitter 3
The three-beam gone out is coupled into three fibre cores of six core fibres 5, and the output light of the other three fibre core of six core fibres 5 is coupled into
In three spectrometer reception optical fiber 11-1；Strong trapping stiffness optical fiber optical tweezers 6 are used for Acquisition Detection cell 12；The micro- behaviour's hand of three-D electric I 7
Position for adjusting six core fibres 5, the micro- behaviour's hand I 7 of three-D electric, which is HS6, has X, Y, Z tri- to be adjusted axially micro- manipulation in direction
Adjusting apparatus, the micro- behaviour's hand II 8 of three-D electric are used to adjust the positions of strong trapping stiffness optical fiber optical tweezers 6, and the micro- behaviour's hand of three-D electric II 8 is
HS6 has micro- manipulation adjusting apparatus that X, Y, Z tri- are adjusted axially direction；Laser light source 10 is for generating Acquisition Detection cell 12
The output end of light, laser light source 10 is provided with light source output optical fiber 10-1；Optical fiber bonding machine 9 is for fixing strong trapping stiffness optical fiber light
The position of the input terminal of tweezer 6 and the output end of light source output optical fiber 10-1；Microscope 13 is used to determine the test side of six core fibres 5
With the position of the output end of strong trapping stiffness optical fiber optical tweezers 6；
The output end of light source 1 is connect with the input terminal of light source tail optical fiber 2, output end and the fiber optic splitter 3 of light source tail optical fiber 2
Input terminal connects, and the output end of fiber optic splitter 3 couples the light into the non-detection end of six core fibres 5 by optical coupling lens group 4
Three fibre cores in, the output light of the other three fibre core of six core fibres 5 is coupled into three root receiving fibers by optical coupling lens group 4
11-1 is simultaneously passed in spectrometer 11, and the test side of six core fibres 5 is connect with the control terminal of the micro- behaviour's hand I 7 of three-D electric, strong trapping stiffness
The control terminal of the micro- behaviour's hand II 8 of the output end of optical fiber optical tweezers 6 and three-D electric connect, the input terminal of strong trapping stiffness optical fiber optical tweezers 6 and
The output end face of light source output optical fiber 10-1 is welded；The test side of six core fibres 5, strong trapping stiffness optical fiber optical tweezers 6 output end
And the Acquisition Detection cell 12 of strong 6 capture of trapping stiffness optical fiber optical tweezers is placed in the eyepiece visual field of microscope 13；Manufacturing process：Take one
The single mode optical fiber of section 30cm-50cm, is cut flat with the fiber end face of optical fiber using optical fiber cutter, and dip alcohol pair with non-woven fabrics
Two fiber end faces carry out wiped clean and the input terminal of light source tail optical fiber 2 are connected to the output of light source 1 as light source tail optical fiber 2
End, the output end of light source tail optical fiber 2 are connected to the input terminal of fiber optic splitter 3；Six core fibres of one section of 30cm-50cm are taken, are utilized
Optical fiber cutter cuts flat with the fiber end face of optical fiber, and dips two fiber end faces of alcohol pair with non-woven fabrics and carry out wiped clean,
As six core fibres 5, the output end of six core fibres 5 is placed on optical fiber cone grinding machine, by face where six fibre cores of six core fibres 5
Three pairs of cone angles are ground respectively, and cone angle model is 8 ° -20 °, is taken out after milled, is used in combination non-woven fabrics to dip alcohol and wipes fiber end face
It wipes totally, places it in small ion sputter, in I 5-1, SPR detection faces of SPR detection faces, II 5-2 and SPR detection faces, III 5-
The nano-gold film of 50nm is set in 3 platings, and the nano-gold film of 300nm, film are set in I 5-4 of reflecting surface, II 5-5 of reflecting surface and reflecting surface III 5-6 platings
Thickness is detected by three-dimensional appearance analyzer, is taken out after plating is good, the non-detection end of six core fibres 5 is received optical coupling lens group 4
The test side of six core fibres 5 is fixed on the control terminal of the micro- behaviour's hand I 7 of three-D electric, is placed in the eyepiece of microscope 13 by output light
In the visual field；The fiber end face of optical fiber is cut flat with using optical fiber cutter, and uses nothing by the annular core fibre for taking one section of 30cm-50cm
Woven fabric dips two fiber end faces of alcohol pair and carries out wiped clean, as strong trapping stiffness optical fiber optical tweezers 6, by strong trapping stiffness optical fiber light
The test side of tweezer 6 is placed on optical fiber cone grinding machine wears into frustum cone structure by output end, and 8 ° -20 ° of the angular range of round platform grinds and takes
Go out, the input terminal of strong trapping stiffness optical fiber optical tweezers 6 is placed in optical fiber bonding machine 9 and is welded with light source output optical fiber 10-1 faces, it will
For the output end of strong trapping stiffness optical fiber optical tweezers 6 by adjusting the micro- X for grasping hand II 8 of three-D electric, tri- directions Y, Z make strong trapping stiffness
The output end of optical fiber optical tweezers 6 is placed in the eyepiece visual field of microscope 13, and Acquisition Detection cell 12, adjusts the micro- behaviour's hand of three-D electric
Deeply detect the inside of cell 12 in the test side of I 7 X, Y, tri- direction controllings of Z, six core fibre 5；By the switch of laser light source 10
It opens, the light of light source 10 is inputted by light source output optical fiber 10-1 in strong trapping stiffness optical fiber optical tweezers 6, and light is in strong trapping stiffness optical fiber light
The output end of tweezer 6 generates convergence, and the region of light convergence generates, and there is trapping stiffness, trapping stiffness size to be enough Acquisition Detection cell 12,
By the X of the micro- behaviour's hand II 8 of three-D electric, the position of control terminal is adjusted on tri- adjustable directions by Y, Z, and then changes strong ligh trap
Strong trapping stiffness optical fiber optical tweezers 6 are placed in the eyepiece region of microscope 13, and adjust three-D electric by the position of power optical fiber optical tweezers 6
The control terminal of micro- behaviour's hand II 8 makes strong trapping stiffness optical fiber optical tweezers 6 capture detection cell 12 to be measured；It is micro- by adjusting three-D electric
The eyepiece that the test side of six core fibres 5 is placed in microscope 13 by behaviour's hand I 7 within sweep of the eye, adjusts the micro- behaviour's hand I 7 of three-D electric and controls
The test side for making six core fibres 5 is inserted into detection cell 12；The switch of light source 1 and spectrometer 11 is opened, the light of light source 1 passes through light
Source tail optical fiber 2 is passed to the input terminal of fiber optic splitter 3, and light is divided into three-beam and exported by fiber optic splitter 3, fiber optic splitter 3
Output light is coupled by optical coupling lens group 4 in three fibre cores of six core fibres 5, optical transport to six core fibres, 5 test side
I 5-1, SPR detection faces of SPR detection faces, II 5-2 and SPR detection faces, III 5-3, light generate suddenly in fibre core and the interface of nano-gold film
Die wave, and the free electron in evanescent wave in the light and nano-gold film of a certain frequency resonates, the energy of the light of the resonant frequency
It is absorbed by free electron, energy is substantially decayed, and SPR sensorgram spectrum, SPR are generated under the action of detecting the intracellular fluid of cell 12
Sense the other three fibre core for being reflected back six core fibres 5 that spectrum passes through III 5-6 of I 5-4 of reflecting surface, II 5-5 of reflecting surface and reflecting surface
In, SPR sensorgram spectrum is coupled by optical coupling lens group 4 in spectrometer reception optical fiber 11-1, is finally transmitted to spectrometer 11
In, three groups of detection spectrum can be obtained, by by detection light it is observed that SPR sensorgram spectrum detects spectrum in spectrometer 11 altogether
Spectrum comparison normalization so that testing result is more acurrate；Use the switch for finishing closing light source 1, laser light source 10 and spectrometer 11
.
A kind of medical unicellular inner wall detection device of the utility model, the light source tail optical fiber 2 are single mode optical fiber or few mould
Optical fiber.
A kind of medical unicellular inner wall detection device of the utility model, I 5-1, SPR detection faces II of SPR detection faces
The surface of III 5-3 of 5-2 and SPR detection faces is coated with the nano-gold film of 50nm respectively, and experiment shows that the SPR of the nano-gold film of 50nm is passed
It is best to feel effect.
A kind of medical unicellular inner wall detection device of the utility model, I 5-4 of reflecting surface, II 5-5 of reflecting surface and anti-
The surface for penetrating III 5-6 of face is coated with the nano-gold film of 300nm respectively, and thick nano-gold film can not excite SPR phenomenons, can reflect SPR
Sense spectrum.
A kind of medical unicellular inner wall detection device of the utility model, six fibre cores of six core fibres 5 are distributed in
It is on one concentric circles and symmetrical two-by-two, three pairs of detection faces are formed by six fibre cores of six core fibres 5, it can be to detecting cell 10
Inside different direction be detected, improve the accuracy of testing result.
A kind of medical unicellular inner wall detection device of the utility model, the strong trapping stiffness optical fiber optical tweezers 6 are ring light
Fine optical tweezer.
A kind of medical unicellular inner wall detection device of the utility model, the laser light source 10 are the laser light of 532nm
The laser light source of source or 980nm are strong using laser light source trapped particle capture ability.
A kind of medical unicellular inner wall detection device of the utility model, the light source output optical fiber 10-1 are single mode optical fiber
Or less fundamental mode optical fibre, single mode optical fiber or less fundamental mode optical fibre light loss are small.
A kind of medical unicellular inner wall detection device of the utility model, the spectrometer reception optical fiber 11-1 are that step is more
The big receipts light ability of core diameter of mode fiber or gradual change multimode fibre, the optical fiber of multimode fibre is strong.
The operation principle of the utility model is：
The switch of laser light source 10 is opened, the light of light source 10 inputs strong trapping stiffness optical fiber by light source output optical fiber 10-1
In optical tweezer 6, light generates convergence in the output end of strong trapping stiffness optical fiber optical tweezers 6, and the region of light convergence, which generates, has trapping stiffness, ligh trap
Power size is enough Acquisition Detection cell 12, by the X of the micro- behaviour's hand II 8 of three-D electric, by the position of control terminal on the tri- adjustable directions Y, Z
It sets and is adjusted, and then change the position of strong trapping stiffness optical fiber optical tweezers 6, strong trapping stiffness optical fiber optical tweezers 6 are placed in microscope 13
In eyepiece region, and the control terminal for adjusting the micro- behaviour's hand II 8 of three-D electric makes strong trapping stiffness optical fiber optical tweezers 6 capture detection to be measured
The test side of six core fibres 5 is placed in the eyepiece field range of microscope 13 by the micro- behaviour's hand I 7 of adjusting three-D electric by cell 12
It is interior, it adjusts the micro- behaviour's hand I 7 of three-D electric and controls in the test side insertion detection cell 12 of six core fibres 5；Open light source 1 and spectrum
The switch of instrument 11, the light of light source 1 are passed to the input terminal of fiber optic splitter 3 by light source tail optical fiber 2, and light is divided by fiber optic splitter 3
Three-beam simultaneously exports, and the output light of fiber optic splitter 3 is coupled by optical coupling lens group 4 in three fibre cores of six core fibres 5,
Optical transport is to I 5-1, SPR detection faces of SPR detection faces, II 5-2 and SPR detection faces, III 5-3 of six core fibres, 5 test side, and light is in fibre
Core and the interface of nano-gold film generate evanescent wave, and the free electron in evanescent wave in the light and nano-gold film of a certain frequency occurs
Resonance, the energy of the light of the resonant frequency are absorbed by free electron, and energy is substantially decayed, and are detecting the intracellular fluid of cell 12
Effect is lower to generate SPR sensorgram spectrum, and SPR sensorgram spectrum is anti-by III 5-6's of I 5-4 of reflecting surface, II 5-5 of reflecting surface and reflecting surface
It is emitted back towards in the other three fibre core of six core fibres 5, SPR sensorgram spectrum is coupled into spectrometer reception optical fiber by optical coupling lens group 4
It in 11-1, is finally transmitted in spectrometer 11, spectrometer 11 is available altogether it is observed that SPR sensorgram spectrum i.e. detection spectrum
Three groups of detection spectrum, by the way that spectrum comparison normalization will be detected so that testing result is more acurrate；Using finish close light source 1, swash
The switch of radiant 10 and spectrometer 11.
Certainly, above description is not limitation of the utility model, and the utility model is also not limited to the example above, this skill
The variations, modifications, additions or substitutions that the those of ordinary skill in art field is made in the essential scope of the utility model, also belong to
In the scope of protection of the utility model.
Claims (9)
1. a kind of medical unicellular inner wall detection device, it is characterised in that：The medical unicellular rotatable multi-direction detection device
Including light source (1), light source tail optical fiber (2), fiber optic splitter (3), optical coupling lens group (4), six core fibres (5), strong trapping stiffness light
Fine optical tweezer (6), the micro- behaviour's hand I (7) of three-D electric, the micro- behaviour's hand II (8) of three-D electric, laser light source (10), spectrometer (11) and aobvious
Micro mirror (13)；
Light source (1) is the super continuum source of spectral width 450nm -1100nm, the exciting light for generating excitation SPR phenomenons；
Light source tail optical fiber (2) is used for transmission the light of light source (1)；Fiber optic splitter (3) is used to the light of light source (1) being divided into three beams；Spectrometer
(11) spectral width is 450nm -1100nm, for receiving, preserving and showing SPR sensorgram spectrum, the input of spectrometer (11)
End is provided with spectrometer reception optical fiber (11-1)；Six core fibres (5) are for detecting detection cell (12), the inspection of six core fibres (5)
It surveys end and is provided with SPR detection faces I (5-1), SPR detection faces II (5-2), SPR detection faces III (5-3), reflecting surface I (5-4), reflection
Face II (5-5) and reflecting surface III (5-6), it is SPR detection faces I (5-1), SPR detection faces II (5-2), SPR detection faces III (5-3), anti-
It includes a fibre core, SPR detection faces I (5-1) and reflecting surface to penetrate face I (5-4), reflecting surface II (5-5) and reflecting surface III (5-6) respectively
I (5-4) relatively, SPR detection faces II (5-2) and reflecting surface II (5-5) relatively, III (5- of SPR detection faces III (5-3) and reflecting surface
6) opposite；Optical coupling lens group (4) is used to the three-beam that fiber optic splitter (3) separates being coupled into three of six core fibres (5)
In fibre core, and the output light of the other three fibre core of six core fibres (5) is coupled into three spectrometer reception optical fibers (11-1)；By force
Trapping stiffness optical fiber optical tweezers (6) are used for Acquisition Detection cell (12)；The micro- behaviour's hand I (7) of three-D electric is for adjusting six core fibres (5)
Position, the micro- behaviour's hand II (8) of three-D electric are used to adjust the position of strong trapping stiffness optical fiber optical tweezers (6)；Laser light source (10) is for producing
The light of raw Acquisition Detection cell (12), the output end of laser light source (10) are provided with light source output optical fiber (10-1)；Microscope
(13) it is used to determine the position of the test side of six core fibres (5) and the output end of strong trapping stiffness optical fiber optical tweezers (6)；
The output end of light source (1) is connect with the input terminal of light source tail optical fiber (2), the output end and fiber optic splitter of light source tail optical fiber (2)
(3) input terminal connection, the output end of fiber optic splitter (3) couple the light into six core fibres (5) by optical coupling lens group (4)
Non-detection end three fibre cores in, the output light of the other three fibre core of six core fibres (5) is coupled by optical coupling lens group (4)
In three root receiving fibers (11-1) and incoming spectrometer (11), test side and the micro- behaviour's hand I (7) of three-D electric of six core fibres (5)
Control terminal connection, the output ends of strong trapping stiffness optical fiber optical tweezers (6) connect with the micro- control terminal for grasping hand II (8) of three-D electric, by force
The input terminal of trapping stiffness optical fiber optical tweezers (6) is welded with the output end face of light source output optical fiber (10-1)；The inspection of six core fibres (5)
The Acquisition Detection cell (12) for surveying end, the output end of strong trapping stiffness optical fiber optical tweezers (6) and strong trapping stiffness optical fiber optical tweezers (6) capture is set
In the eyepiece visual field of microscope (13).
2. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The light source tail optical fiber
(2) it is single mode optical fiber or less fundamental mode optical fibre.
3. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The SPR detections
The surface in face I (5-1), SPR detection faces II (5-2) and SPR detection faces III (5-3) is coated with the nano-gold film of 50nm respectively.
4. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The reflecting surface I
The surface of (5-4), reflecting surface II (5-5) and reflecting surface III (5-6) are coated with the nano-gold film of 300nm respectively.
5. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：Six core fibres
(5) six fibre cores are distributed on a concentric circles and symmetrical two-by-two.
6. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The strong trapping stiffness
Optical fiber optical tweezers (6) are optical fibre ring optical tweezer.
7. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The laser light source
(10) laser light source of the laser light source or 980nm that are 532nm.
8. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The light source output
Optical fiber (10-1) is single mode optical fiber or less fundamental mode optical fibre.
9. the medical unicellular inner wall detection device of one kind according to claim 1, it is characterised in that：The spectrometer connects
It is step multimode fibre or gradual change multimode fibre to receive optical fiber (11-1).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109870431A (en) * | 2019-02-25 | 2019-06-11 | 桂林电子科技大学 | Multi-core optical fiber cell sensor with cone rotary table fibre end structure |
| CN109946271A (en) * | 2019-02-25 | 2019-06-28 | 桂林电子科技大学 | A kind of multi-core optical fiber cell sensor with non-equilateral pyramid optical fiber end structure |
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2018
- 2018-03-19 CN CN201820370642.4U patent/CN207976390U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109870431A (en) * | 2019-02-25 | 2019-06-11 | 桂林电子科技大学 | Multi-core optical fiber cell sensor with cone rotary table fibre end structure |
| CN109946271A (en) * | 2019-02-25 | 2019-06-28 | 桂林电子科技大学 | A kind of multi-core optical fiber cell sensor with non-equilateral pyramid optical fiber end structure |
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Effective date of registration: 20190802 Address after: 311200 Zidong Village, Heshang Town, Xiaoshan District, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Sangjiefei Technology Co., Ltd. Address before: 318000 Xiabeishan Village 89, Wanyuan Street, Jiaojiang District, Taizhou City, Zhejiang Province Patentee before: Zhang Biao |