CN208060385U - A kind of medical unicellular rotatable multi-direction detection device - Google Patents
A kind of medical unicellular rotatable multi-direction detection device Download PDFInfo
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- CN208060385U CN208060385U CN201820371378.6U CN201820371378U CN208060385U CN 208060385 U CN208060385 U CN 208060385U CN 201820371378 U CN201820371378 U CN 201820371378U CN 208060385 U CN208060385 U CN 208060385U
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Abstract
The utility model provides a kind of medical unicellular rotatable multi-direction detection device, the device forms three pairs of optical tweezers by six fibre cores of six core fibres, by controlling different optical tweezer work, it realizes unicellular captured with different postures, and then the characteristic of single celled different directions is detected, testing result is more acurrate.The device includes laser light source, light source tail optical fiber, fiber optic splitter, optical coupling lens group, six core fibres, twin-core fiber, the micro- behaviour's hand I of three-D electric, the micro- behaviour's hand II of three-D electric, super continuum source, spectrometer and microscope；In the light input optical fibre beam splitter of laser light source, the output light of fiber optic splitter is coupled the light by optical coupling lens group in each fibre core of six core fibres, the micro- behaviour's hand I of three-D electric controls the output end of six core fibres, the micro- behaviour's hand II of three-D electric controls the test side of twin-core fiber, the light of super continuum source is passed to the SPR detection faces of twin-core fiber, and the reflective surface of twin-core fiber detects spectrum incoming light spectrometer.
Description
Technical field
The utility model is related to medical sciences, are in particular a kind of medical unicellular rotatable multi-direction detections
Device.
Background technology
Cell Measurement Technique has developed some time, but can all occur it in annual Nature Methods prospect technologies
Figure, this is mainly due to the cells in culture medium or body there are diversity, or perhaps heterogeneous, this is many realities
It tests analysis and causes obstacle, therefore with the development of modern biology, " average value " this word cannot meet the need of people
It has been wanted that, people are it is to be understood that otherness between cell.If various unicellular collection of illustrative plates can be integrated, researcher can be helped
Understand gene regulation and the heterogeneous mechanism of action of gene, small due to cell, detection is more difficult, detection it is accurately relatively low, because
This cell detection device extremely has research necessary.Optical fiber has obtained answering extensively in biomedical, sensing, communication and the fields such as detection
There are many merits as transmission medium with, optical fiber, such as electrical insulating property is good, chemical property is stable, transmission speed is fast, and can be
It works under the rugged environments such as inflammable and explosive, toxic, high temperature and pressure, with wide Research Prospects.Optical fiber surface plasmon
Resonance body (Surface Plasmon Resonance, SPR) sensor be mainly to the variation of a certain physical quantity of external environment into
Row perception and transmission, physical quantity can be temperature, liquid refractivity, pressure etc..With the development and progress of society, to people couple
The research of cell is more and more deep, is detected to cell by optical fiber and is also budded out into popularity, and the utility model provides a kind of doctor
With unicellular rotatable multi-direction detection device, which forms three pairs of optical tweezers, realization pair by six fibre cores of six core fibres
Single celled capture controls different fibre core thang-kngs, realizes different optical tweezer work, can control single celled rotation, and then realize
To the Characteristics Detection of single celled different directions so that testing result is more acurrate, solves Single cell analysis and is difficult to control, detection
As a result the problems such as accuracy is inadequate.
Invention content
The utility model provides a kind of medical unicellular rotatable multi-direction detection device, which passes through six core fibres
Six fibre cores form three pairs of optical tweezers, realize to single celled capture, control different fibre core thang-kngs, realize the work of different optical tweezers,
Single celled rotation can be controlled, and then realizes the Characteristics Detection to single celled different directions so that testing result is more acurrate,
Solves the problems such as Single cell analysis is not easy to control, and testing result accuracy is inadequate.
To achieve the above object, the utility model provides a kind of medical unicellular rotatable multi-direction detection device, should
Medical unicellular rotatable multi-direction detection device include laser light source, light source tail optical fiber, fiber optic splitter, optical coupling lens group,
The micro- behaviour's hand I of six core fibres, twin-core fiber, three-D electric, the micro- behaviour's hand II of three-D electric, super continuum source, spectrometer and micro-
Mirror；
Laser light source is used to generate the light of Acquisition Detection cell；Light source tail optical fiber is used for transmission the light of laser light source；Optical fiber point
Beam device is used to the light of laser light source being divided into six beams；Optical coupling lens group be used for the six beam optical couplings that separate fiber optic splitter into
In six fibre cores of six core fibres, six core fibres are for Acquisition Detection cell and enable its rotation, the test side setting of twin-core fiber
Have SPR detection faces and a reflecting surface, SPR detection faces and reflecting surface include respectively a fibre core, SPR detection faces be used for detection cell into
Row detection, excitation SPR phenomenons generate SPR sensorgram spectrum, and reflecting surface will be for that will reflect SPR sensorgram spectrum；The micro- behaviour's hand of three-D electric I
Position for adjusting six core fibres, the micro- behaviour's hand II of three-D electric are used to adjust the position of twin-core fiber；Super continuum source
Output end is provided with light source output optical fiber, and super continuum source is the super continuum source of spectral width 450nm -1100nm, is used
In the exciting light for generating excitation SPR phenomenons；The input terminal of spectrometer is provided with spectrometer reception optical fiber, the spectral width of spectrometer
For 450nm -1100nm, for receiving, preserving and show SPR sensorgram spectrum；
The output end of laser light source is connect with the input terminal of light source tail optical fiber, the output end of light source tail optical fiber and fiber optic splitter
Input terminal connects, and the output end of fiber optic splitter couples the light into each of the input terminal of six core fibres by optical coupling lens group
In fibre core, the output end of six core fibres is connect with the control terminal of the micro- behaviour's hand I of three-D electric, six core fibres and its Acquisition Detection cell
It is placed in the microscopical eyepiece visual field, the test side of twin-core fiber is connect with the control terminal of the micro- behaviour's hand II of three-D electric, twin-core light
The output end at fine non-detection end, light source output optical fiber and the input terminal face welding of spectrometer reception optical fiber, SPR detection faces and
Reflecting surface is placed in the microscopical eyepiece visual field, and the SPR detection faces of the incoming twin-core fiber of output light of light source output optical fiber include
Fibre core in, the output light for the fibre core that the reflecting surface of twin-core fiber includes is passed in spectrometer reception optical fiber.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the laser light source is the laser light source of 532nm or the laser light source of 980nm.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the light source tail optical fiber is single mode optical fiber or less fundamental mode optical fibre.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the light source output optical fiber is single mode optical fiber or less fundamental mode optical fibre.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the spectrometer reception optical fiber is step multimode fibre or gradual change multimode fibre.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the SPR detection faces are coated with the nano-gold film of 50nm.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, the reflecting surface is coated with the nano-gold film of 300nm.
As advanced optimizing for the utility model, a kind of medical unicellular rotatable multi-direction detection dress of the utility model
It sets, six fibre cores of six core fibres are distributed on a concentric circles and symmetrical two-by-two.
A kind of medical unicellular rotatable multi-direction detection device of the utility model has the beneficial effect that：
1. the utility model provides a kind of medical unicellular rotatable multi-direction detection device, which passes through six core light
Six fine fibre cores form three pairs of optical tweezers, realize to single celled capture, control different fibre core thang-kngs, realize different optical tweezer works
Make, single celled rotation can be controlled, and then realize the Characteristics Detection to single celled different directions so that testing result is more accurate
Really, the problems such as Single cell analysis is not easy to control, and testing result accuracy is inadequate is solved.
2. the utility model can be made up of three pairs of optical tweezers six fibre cores of six core fibres, realization is caught to single celled
It obtains so that detection cell is not destroyed, and improves the accuracy of experiment.
3. the utility model realizes different optical tweezer work, can control single celled rotation by controlling different fibre core thang-kngs
Turn, and then realize to the Characteristics Detection of single celled different directions, improves the accuracy of testing result.
4. the utility model uses optical fiber sensing principle, very sensitively single celled characteristic can be sensed,
It is strong to different single celled characteristic variations sensing capabilities, there is higher detection sensitivity.
5. the utility model transmits testing result by optical fiber, detection speed is fast, and detection efficiency is high.
6. a kind of medical unicellular rotatable multi-direction detection device of the utility model is simple for structure, device materials are easy
It seeks, it is cost-effective, it is easy to operate.
7. the utility model can be repeatedly used with continuous work, cost-effective, highly reliable.
Description of the drawings
The utility model is described in more detail with specific implementation method below in conjunction with the accompanying drawings.
Fig. 1 is a kind of structural schematic diagram of medical unicellular rotatable multi-direction detection device of the utility model；
Fig. 2 is a kind of showing for the SPR detection zones detection of medical unicellular rotatable multi-direction detection device of the utility model
It is intended to；
Fig. 3 is the enlarged diagram that the utility model SPR detection faces carry out Single cell analysis；
Fig. 4 is the schematic diagram of the optical coupling of the utility model optical coupling lens group；
Fig. 5 is the schematic diagram of the utility model unicellular I；
Fig. 6 is the schematic diagram of the utility model unicellular II.
In figure：Laser light source 1；Light source tail optical fiber 2；Fiber optic splitter 3；Optical coupling lens group 4；Six core fibres 5；Twin-core fiber
6；SPR detection faces 6-1；Reflecting surface 6-2；The micro- behaviour's hand of three-D electric I 7；The micro- behaviour's hand of three-D electric II 8；Optical fiber bonding machine 9；It is super to connect
Continuous spectrum light source 10；Light source output optical fiber 10-1；Spectrometer 11；Spectrometer reception optical fiber 11-1；Detect cell 12；Unicellular I 12-
1；Unicellular II 12-2；Microscope 13.
Specific implementation mode
Illustrate present embodiment with reference to Fig. 1,2,3,4,5,6, the utility model provide it is a kind of it is medical it is unicellular can
Multi-direction detection device is rotated, which forms three pairs of optical tweezers by six fibre cores of six core fibres, and realization is caught to single celled
It obtains, controls different fibre core thang-kngs, realize different optical tweezer work, single celled rotation can be controlled, and then realize to single celled
The Characteristics Detection of different directions so that testing result is more acurrate, solves Single cell analysis and is difficult to control, testing result accuracy
The problems such as inadequate.
To achieve the above object, the utility model provides a kind of medical unicellular rotatable multi-direction detection device, should
Medical unicellular rotatable multi-direction detection device includes laser light source 1, light source tail optical fiber 2, fiber optic splitter 3, optical coupling lens
4, six core fibres 5 of group, twin-core fiber 6, the micro- behaviour's hand I 7 of three-D electric, the micro- behaviour's hand II 8 of three-D electric, super continuum source 10, light
Spectrometer 11 and microscope 13；
Laser light source 1 is used to generate the light of Acquisition Detection cell 12；Light source tail optical fiber 2 is used for transmission the light of laser light source 1；Light
Fine beam splitter 3 is used to the light of laser light source 1 being divided into six beams；Six beams that optical coupling lens group 4 is used to separate fiber optic splitter 3
For optical coupling into six fibre cores of six core fibres 5, six core fibres 5 are for Acquisition Detection cell 12 and enable its rotation, twin-core fiber 6
Test side be provided with SPR detection faces 6-1 and reflecting surface 6-2, SPR detection faces 6-1 and reflecting surface 6-2 respectively include a fibre core,
SPR detection faces 6-1 is used to be detected detection cell 12, and excitation SPR phenomenons generate SPR sensorgram spectrum, and reflecting surface 6-2 is used for
SPR sensorgram spectrum will be reflected；The micro- behaviour's hand I 7 of three-D electric is used to adjust the positions of six core fibres 5, and the micro- behaviour's hand of three-D electric I 7 is
HS6 has micro- manipulation adjusting apparatus that X, Y, Z tri- are adjusted axially direction；The micro- behaviour's hand II 8 of three-D electric is for adjusting twin-core fiber 6
Position, the micro- behaviour's hand II 8 of three-D electric, which is HS6, micro- manipulation adjusting apparatus that X, Y, Z tri- are adjusted axially direction；Super continuum source
10 output end is provided with light source output optical fiber 10-1, and super continuum source 10 is the super company of spectral width 450nm -1100nm
Continuous spectrum light source, the exciting light for generating excitation SPR phenomenons, super continuum source 10 are LDLS systems of Energetiq companies of the U.S.
Arrange the super continuum source of EQ-99FC；The input terminal of spectrometer 11 is provided with spectrometer reception optical fiber 11-1, the light of spectrometer 11
Spectral width is 450nm -1100nm, for receiving, preserving and showing that SPR sensorgram spectrum, spectrometer 11 are Japanese Yokogawa
AQ6370C spectrometers；
The output end of laser light source 1 is connect with the input terminal of light source tail optical fiber 2, the output end and fiber beam splitting of light source tail optical fiber 2
The input terminal of device 3 connects, and laser light source 1 is LR-ISP-980 laser light sources, and the output end of fiber optic splitter 3 is saturating by optical coupling
Microscope group 4 couples the light into each fibre core of the input terminal of six core fibres 5, output end and the micro- behaviour of three-D electric of six core fibres 5
The control terminal of hand I 7 connects, and six core fibres 5 are placed in its Acquisition Detection cell 12 in the eyepiece visual field of microscope 13, twin-core fiber
6 test side is connect with the control terminal of the micro- behaviour's hand II 8 of three-D electric, the non-detection end of twin-core fiber 6, light source output optical fiber 10-1
Output end and the input terminal of spectrometer reception optical fiber 11-1 welded using 9 face of optical fiber bonding machine, SPR detection faces 6-1 and anti-
The face 6-2 of penetrating is placed in the eyepiece visual field of microscope 13, and the output light of light source output optical fiber 10-1 is passed to the SPR inspections of twin-core fiber 6
In the fibre core that survey face 6-1 includes, the output light for the fibre core that the reflecting surface 6-2 of twin-core fiber 6 includes is passed to spectrometer reception optical fiber
In 11-1；Manufacturing process：The single mode optical fiber for taking one section of 30cm-50cm is cut flat with the fiber end face of optical fiber using optical fiber cutter,
And dip two fiber end faces of alcohol pair with non-woven fabrics and carry out wiped clean, as light source tail optical fiber 2, by the input of light source tail optical fiber 2
End is connected to the output end of laser light source 1, and the output end of light source tail optical fiber 2 is connected to the input terminal of fiber optic splitter 3；Take one section
The fiber end face of optical fiber is cut flat with using optical fiber cutter, and dips alcohol pair two with non-woven fabrics by six core fibres of 30cm-50cm
A fiber end face carries out wiped clean and the output end of six core fibres 5 is placed on optical fiber cone grinding machine, by six as six core fibres 5
Face where six fibre cores of core fibre 5 grinds three pairs of cone angles respectively, and cone angle model is 8 ° -20 °, is taken out after milled, non-woven fabrics is used in combination
It dips alcohol and wiped clean is carried out to fiber end face, the input terminal of six core fibres 5 is received to the output light of optical coupling lens group 4,
The output end of six core fibres 5 is fixed on to the control terminal of the micro- behaviour's hand I 7 of three-D electric, is placed in the eyepiece visual field of microscope 13；
The fiber end face of optical fiber is cut flat with using optical fiber cutter, and dips wine with non-woven fabrics by the twin-core fiber for taking one section of 30cm-50cm
Two fiber end faces of essence pair carry out wiped clean and the test side of twin-core fiber 6 are placed in optical fiber cone grinding machine as twin-core fiber 6
On face where two fibre cores ground into a pair of of cone angle structure, 8 ° -20 ° of cone angle range, respectively as SPR detection faces 6-1 and reflection
Face 6-2 takes out after milled, places it in small ion sputter, sets the nano-gold film of 50nm in the 6-1 platings of SPR detection faces, instead
The nano-gold film that 300nm is set in 6-2 platings in face is penetrated, film thickness is detected by three-dimensional appearance analyzer, is taken out after plating is good, by twin-core fiber 6
Test side be placed in the eyepiece visual field of microscope 13, the non-detection end of twin-core fiber 6 is placed in defeated with light source in optical fiber bonding machine 9
The position for going out optical fiber 10-1 and spectrometer reception optical fiber 11-1 is fixed；Operation principle：The switch of laser light source 1 will be opened, is swashed
The light of radiant 1 reaches the input terminal of fiber optic splitter 3 by light source tail optical fiber 2, and light beam is divided into six beams simultaneously by fiber optic splitter 3
It is exported by the output end of fiber optic splitter 3, six beam output lights of fiber optic splitter 3 are coupled respectively by optical coupling lens group 4
Into in six fibre cores of six core fibres 5, the light in the fibre core of six core fibres 5 is converged in the output end of six core fibres 5, light convergence
Region generates, and there is trapping stiffness, trapping stiffness size to be enough Acquisition Detection cell 12, by adjusting the X of the micro- behaviour's hand I 7 of three-D electric,
Tri- positions for adjusting direction and then controlling the output end of six core fibres 5 Y, Z, so that six core fibres under the cooperation of microscope 13
5 Acquisition Detection cells 12, and the output end of six core fibres 5 is placed in the eyepiece visual field of microscope 13, and six core fibres 5 are defeated
The detection cell 12 of outlet capture is also placed in the eyepiece visual field of microscope 13；Open super continuum source 10 and spectrometer 11
Switch, the light of super continuum source 10 inputs the SPR detection faces 6-1 of twin-core fiber 6 by light source output optical fiber 10-1, micro-
The X of the micro- behaviour's hand II 8 of three-D electric is adjusted under the cooperation of mirror 13, Y, Z tri- adjusts direction and the test side of twin-core fiber 6 is placed in inspection
Survey cell 12 near, light SPR detection faces 6-1 fibre core and nano-gold film interface generate evanescent wave, evanescent wave it is a certain
The light of frequency is consistent with the frequency of the free electron of nano-gold film to resonate, and the energy of the light of the resonant frequency is by free electron
It absorbs, energy is substantially decayed, and SPR resonance spectrums are generated under the action of detecting cell 12, and SPR resonance spectrums pass through reflecting surface 6-
The 2 spectrometer reception optical fiber 11-1 that is reflected through is passed in spectrometer 11；The shadows such as the characteristic such as refractive index due to detection cell 10
Ring the resonant frequency of evanescent wave and the free electron of nano-gold film, the composition and ratio of unicellular I 12-1 and unicellular II 12-2
Difference all affects the characteristic of cell, and SPR resonance spectrums are very sensitive to the change of the characteristic of cell, and detection cell may be implemented
10 with the one-to-one correspondence of SPR resonance spectrums, and then realize the detection of detection cell 10；Since six core fibres 5 have three pairs of optical tweezers,
If removing two pairs of output lights of fiber optic splitter 3, another pair optical tweezer work, therefore cell 10 is detected with a kind of posture quilt
Capture, twin-core fiber 6 are detected detection cell 10, this optical tweezer is removed injection light, then detect cell 10 and dissociate, will be another
A pair of of fibre core thang-kng of one optical tweezer is then detected cell 10 and is captured again with a kind of new posture under free state, double
Core fibre 6 re-starts detection to detection cell 10, and similarly to optical tweezer, therefore the detection cell 10 is in the state of rotation for third
Multiple faces are all detected, and testing result is normalized, and it is more accurate that the testing result of gained more only detects one side；It uses
Finish the switch for closing laser light source 1, super continuum source 10 and spectrometer 11.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the laser light source 1 is 532nm
Laser light source or 980nm laser light source, it is strong using laser light source trapped particle capture ability, laser light source 1 be LR-ISP-
980 laser light sources.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the light source tail optical fiber 2 are single-mode optics
Fine or less fundamental mode optical fibre, single mode optical fiber or less fundamental mode optical fibre light loss are small.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the light source output optical fiber 10-1 are
Single mode optical fiber or less fundamental mode optical fibre, single mode optical fiber or less fundamental mode optical fibre light loss are small.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the spectrometer reception optical fiber 11-1
For step multimode fibre or gradual change multimode fibre.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the SPR detection faces 6-1 are coated with
The nano-gold film of 50nm, experiment show that the SPR sensorgram effect of the nano-gold film of 50nm is best.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, the reflecting surface 6-2 are coated with 300nm
Nano-gold film.
A kind of medical unicellular rotatable multi-direction detection device of the utility model, six fibres of six core fibres 5
Core is distributed on a concentric circles and symmetrical two-by-two, and three optical tweezers are formed by six fibre cores of six core fibres 5, can be to detection
The capture control of cell 10 freely, by controlling different fibre core thang-kngs, realizes different optical tweezer work, can control single celled rotation
Turn, and then realize to the Characteristics Detection of single celled different directions, improves the accuracy of testing result.
The operation principle of the utility model is：
The switch of laser light source 1 is opened, the light of laser light source 1 reaches the input of fiber optic splitter 3 by light source tail optical fiber 2
Light beam is divided into six beams and is exported by the output end of fiber optic splitter 3, six beams of fiber optic splitter 3 by end, fiber optic splitter 3
Output light is coupled by optical coupling lens group 4 in six fibre cores of six core fibres 5 respectively, the light in the fibre core of six core fibres 5
It is converged in the output end of six core fibres 5, the region of light convergence generates, and there is trapping stiffness, trapping stiffness size to be enough Acquisition Detection cell
12, by adjusting the X of the micro- behaviour's hand I 7 of three-D electric, tri- positions for adjusting direction and then controlling the output end of six core fibres 5 Y, Z
It sets, six core fibres, 5 Acquisition Detection cell 12 so that under the cooperation of microscope 13, and the output end of six core fibres 5 is placed in
In the eyepiece visual field of microscope 13, and the detection cell 12 of six core fibres, 5 output end capture is also placed in the eyepiece of microscope 13 and regards
Yezhong；The switch of super continuum source 10 and spectrometer 11 is opened, the light of super continuum source 10 passes through light source output optical fiber 10-
The SPR detection faces 6-1 of 1 input twin-core fiber 6, adjust the X, Y, Z tri- of the micro- behaviour's hand II 8 of three-D electric under the cooperation of microscope 13
The test side of twin-core fiber 6 is placed near detection cell 12 by a adjusting direction, fibre core and nanometer of the light in SPR detection faces 6-1
The interface of golden film generates evanescent wave, and the light of a certain frequency of evanescent wave is consistent with the frequency of the free electron of nano-gold film to be occurred
Resonance, the energy of the light of the resonant frequency are absorbed by free electron, and energy is substantially decayed, and is generated under the action of detecting cell 12
SPR resonance spectrums, SPR resonance spectrums are passed to spectrometer 11 by the spectrometer reception optical fiber 11-1 that is reflected through of reflecting surface 6-2
In；It is single since the characteristic such as refractive index etc. of detection cell 10 influences the resonant frequency of evanescent wave and the free electron of nano-gold film
The composition and ratio difference of I 12-1 of cell and unicellular II 12-2 all affect the characteristic of cell, and SPR resonance spectrums are to cell
The change of characteristic is very sensitive, and the one-to-one correspondence of detection cell 10 and SPR resonance spectrums may be implemented, and then realizes detection cell
10 detection；Since six core fibres 5 have three pairs of optical tweezers, if removing two pairs of output lights of fiber optic splitter 3, another pair
Optical tweezer works, therefore detects cell 10 and be captured with a kind of posture, and twin-core fiber 6 is detected detection cell 10, by this
Optical tweezer removes injection light, then detects cell 10 and dissociate, and by a pair of of fibre core thang-kng of another optical tweezer, then detects cell 10 free
It is captured again with a kind of new posture under state, twin-core fiber 6 re-starts detection to detection cell 10, and third is to optical tweezer
Similarly, therefore the detection cell 10 multiple faces in the state of rotation are all detected, and testing result are normalized, gained
Testing result more only detect one side it is more accurate；Laser light source 1, super continuum source 10 and spectrometer 11 are closed using finishing
Switch.
Certainly, above description is not limitation of the utility model, and the utility model is also not limited to the example above, this skill
The variations, modifications, additions or substitutions that the those of ordinary skill in art field is made in the essential scope of the utility model, also belong to
In the scope of protection of the utility model.
Claims (8)
1. a kind of medical unicellular rotatable multi-direction detection device, it is characterised in that：This is medical unicellular rotatable multi-direction
Detection device includes laser light source (1), light source tail optical fiber (2), fiber optic splitter (3), optical coupling lens group (4), six core fibres
(5), the micro- behaviour's hand I (7) of twin-core fiber (6), three-D electric, the micro- behaviour's hand II (8) of three-D electric, super continuum source (10), spectrum
Instrument (11) and microscope (13)；
Laser light source (1) is used to generate the light of Acquisition Detection cell (12)；Light source tail optical fiber (2) is used for transmission laser light source (1)
Light；Fiber optic splitter (3) is used to the light of laser light source (1) being divided into six beams；Optical coupling lens group (4) is used for fiber optic splitter
(3) for the six beam optical couplings separated into six fibre cores of six core fibres (5), six core fibres (5) are used for Acquisition Detection cell (12)
And its rotation is enabled, the test side of twin-core fiber (6) is provided with SPR detection faces (6-1) and reflecting surface (6-2), SPR detection faces (6-
1) and reflecting surface (6-2) includes respectively a fibre core, and SPR detection faces (6-1) are used to be detected detection cell (12), excite
SPR phenomenons generate SPR sensorgram spectrum, and reflecting surface (6-2) will be for that will reflect SPR sensorgram spectrum；The micro- behaviour's hand I (7) of three-D electric is used
In the position for adjusting six core fibres (5), the micro- behaviour's hand II (8) of three-D electric is used to adjust the position of twin-core fiber (6)；Super continuous spectrums
The output end of light source (10) is provided with light source output optical fiber (10-1), and super continuum source (10) is spectral width 450nm-
The super continuum source of 1100nm, the exciting light for generating excitation SPR phenomenons；The input terminal of spectrometer (11) is provided with spectrum
The spectral width of instrument reception optical fiber (11-1), spectrometer (11) is 450nm -1100nm, for receiving, preserving and show that SPR is passed
Photosensitive spectrum；
The output end of laser light source (1) is connect with the input terminal of light source tail optical fiber (2), output end and the optical fiber point of light source tail optical fiber (2)
The input terminal of beam device (3) connects, and the output end of fiber optic splitter (3) couples the light into six core light by optical coupling lens group (4)
In each fibre core of the input terminal of fine (5), the output end and the control terminal of the micro- behaviour's hand I (7) of three-D electric of six core fibres (5) connect
It connects, six core fibres (5) are placed in its Acquisition Detection cell (12) in the eyepiece visual field of microscope (13), the inspection of twin-core fiber (6)
It surveys end to connect with the control terminal of the micro- behaviour's hand II (8) of three-D electric, the non-detection end of twin-core fiber (6), light source output optical fiber (10-
1) the input terminal face welding of output end and spectrometer reception optical fiber (11-1), SPR detection faces (6-1) and reflecting surface (6-2)
It is placed in the eyepiece visual field of microscope (13), the output light of light source output optical fiber (10-1) is passed to the SPR detections of twin-core fiber (6)
In the fibre core that face (6-1) includes, the output light for the fibre core that the reflecting surface (6-2) of twin-core fiber (6) includes is passed to spectrometer and receives
In optical fiber (11-1).
2. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Laser light source (1) is the laser light source of 532nm or the laser light source of 980nm.
3. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Light source tail optical fiber (2) is single mode optical fiber or less fundamental mode optical fibre.
4. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Light source output optical fiber (10-1) is single mode optical fiber or less fundamental mode optical fibre.
5. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Spectrometer reception optical fiber (11-1) is step multimode fibre or gradual change multimode fibre.
6. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
SPR detection faces (6-1) are coated with the nano-gold film of 50nm.
7. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Reflecting surface (6-2) is coated with the nano-gold film of 300nm.
8. the medical unicellular rotatable multi-direction detection device of one kind according to claim 1, it is characterised in that：Described
Six fibre cores of six core fibres (5) are distributed on a concentric circles and symmetrical two-by-two.
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|---|---|---|---|---|
| CN112834410A (en) * | 2021-01-04 | 2021-05-25 | 桂林电子科技大学 | Sheet light microscopic imaging method and device based on double-core optical fiber light control |
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| CN112834410A (en) * | 2021-01-04 | 2021-05-25 | 桂林电子科技大学 | Sheet light microscopic imaging method and device based on double-core optical fiber light control |
| CN112834410B (en) * | 2021-01-04 | 2022-09-13 | 桂林电子科技大学 | Sheet light microscopic imaging method and device based on double-core optical fiber light control |
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