CN207970497U - A kind of novel tangential flow chromatography device - Google Patents

Abstract

The utility model discloses a kind of novel tangential flow chromatography devices, including shell, head cover, membrane chromatography material bodies and supporter；The head cover is set to the case top, the membrane chromatography material bodies and the supporter are set to the inside of the shell, there are one the supporters for fitting setting between membrane chromatography material bodies described in each two, helical fluid access is provided on the supporter, the initiating terminal of the fluid passage is communicated with injection port, the injection port is set to the housing bottom, and the end of the fluid passage is communicated with outlet, and the outlet is set on the head cover；Novel tangential flow chromatography device provided by the utility model, can realize the separation of big flow, large volume, higher stickiness and higher turbidity sample, and realization cuts that flow velocity degree is uniform, improves the simple degree of Linear Amplifer.

Description

A kind of novel tangential flow chromatography device

Technical field

The utility model is related to slipstream technical field of chromatography, more particularly to a kind of novel tangential flow chromatography device.

Background technology

Currently, chromatographic separation technology has become one of main, most effective separating and purifying technology.It is traditional Chromatographic separation device is divided into three kinds of axial stream, radial flow, slipstream forms.Traditional axial flow chromatography (Axial Flow Chromatography, AFC) separation process generally uses chromatographic column, and injection port is located at chromatographic column both ends, sample with outlet Product are flowed along chromatographic column axial direction, are realized the separation of substance, more satisfactory effect can be obtained when sample size is less, but not It is suitable for the industrialized production of a large amount of samples, with the increase of column diameter or column length, chromatography optimal conditions originally are often It is not suitable for, as a result can not be satisfactory when being especially further amplified compared with high magnification numbe, in addition, long chromatographic column can occupy The space of bigger should not linearly expand.Preparation for Large Scale Biology sample, there is an urgent need to Development of Novel chromatographic techniques.Diameter It is a kind of new chromatographic point to grow up nearly ten years to flow chromatography (Radial Flow Chromatography, RFC) From analytical technology, the design of unique Radial Flow can be improved flow velocity, reduce column pressure, is convenient for Linear Amplifer, blood product, It is widely used in the isolating and purifying of gene engineering product, biological sample and agricultural product etc..Skill is detached with radial flow chromatogram The chromatographic separation technology that art grows up simultaneously also has slipstream chromatographic technique (Tangential Flow Chromatography, TFC), slipstream chromatographic separation technology is the same with radial flow chromatogram isolation technics, has pressure small, can Realize big flow, the higher stickiness of high flow rate and the processing compared with turbid sample, while slipstream chromatographic technique is in vertical and horizontal Linear Amplifer be relatively easy to, space availability ratio is high.Traditional slipstream chromatogram arrangement is based on rolled structure, support therein Body is mostly used to be fabricated to latticed with flexible material, and the membrane chromatography material being prepared is bonded with supporter, after axis curling Sample path is formed, sample flow direction and membrane chromatography material are tangent, realize that the separation of substance, this kind of mode to a certain extent can The enough efficiency for improving processing, but Linear Amplifer, processing big flow sample may be difficult in the presence of some, and flexible material is as internal Supporter needs the more encrypted grid of arrangement, can reduce available inside device if to maintain higher mechanical strength Volume, meanwhile, it is enlarged again later once rolled structure diameter reaches certain degree, difficulty and cost can significantly increase Add.

For traditional coiled slipstream chromatogram arrangement there are the problem of, it is extremely to have to design novel slipstream chromatogram arrangement Necessary, the design of new device, which should be put forth effort on, solves the problems, such as that tangential flow chromatography rolled structure device exists, and improves slipstream Chromatographic separation technology is in the use in the fields such as environmental science, bioengineering, protein engineering, blood product, this is for slipstream The development of chromatography has very important significance.

Utility model content

The purpose of this utility model is to provide a kind of novel tangential flow chromatography device, above-mentioned of the existing technology to solve Problem, can realize the separation of big flow, large volume, higher stickiness and higher turbidity sample, and it is uniform that flow velocity degree is cut in realization, Improve the simple degree of Linear Amplifer.

To achieve the above object, the utility model provides following scheme：

The utility model provides a kind of novel tangential flow chromatography device, including shell, head cover, membrane chromatography material bodies and support Body；The head cover is set to the case top, and the membrane chromatography material bodies and the supporter are set to the interior of the shell Portion, between membrane chromatography material bodies described in each two fitting setting be provided with spiral on the supporter there are one the supporter The initiating terminal of shape fluid passage, the fluid passage is communicated with injection port, and the injection port is set to the housing bottom, described The end of fluid passage is communicated with outlet, and the outlet is set on the head cover.

Preferably, further include a water distributor, the water distributor bottom end is connected to the injection port, the water distributor top with The head cover connects, and cloth sink, the membrane chromatography material bodies and the supporter are vertically arranged on the tube body of the water distributor Center at be provided with concentric water distributing pore, the cloth sink passes through the water distributing pore and is connected to the fluid passage, institute State at water distributing pore be the fluid passage initiating terminal.

Preferably, the injection port is set at the center of the housing bottom.

Preferably, the injection port is set to the edge of the housing bottom.

Preferably, further include a collector pipe, the collector pipe bottom end is connect with the housing bottom, the collector pipe top It is connected to the outlet, catch basin, the membrane chromatography material bodies and the branch is vertically arranged on the tube body of the collector pipe The edge of support body is provided with concentric gully-hole, and the catch basin passes through the gully-hole and connects with the fluid passage It is logical, it is the end of the fluid passage at the gully-hole.

Preferably, multiple ribs are additionally provided on the supporter, it is logical that multiple ribs are set to the fluid Road bottom.

Preferably, the shell is a barrel-like structure, and the head cover is a disc-shaped structure, the head cover and the shell It is bolted to connection.

Preferably, a sealing ring is additionally provided between the case top and the head cover.

Preferably, the membrane chromatography material bodies and the supporter are disc-shaped structure, the membrane chromatography material bodies with The diameter of the supporter is equal with the internal diameter of the shell.

The utility model achieves following advantageous effects compared with the existing technology：

1, novel tangential flow chromatography device provided by the utility model changes sample in traditional coiled slipstream chromatogram arrangement It realizes the separation of sample by the tangent of sample and upper and lower membrane chromatography material with the tangent form of left and right sides membrane material, subtracts The influence factor for having lacked gravity can be good at the homogenization for realizing upper and lower speed.

2, novel tangential flow chromatography device provided by the utility model, can flexible modulation all parts ruler as needed It is very little, realize the separation of big flow, large volume, higher stickiness and higher turbidity sample, it is uniform that flow velocity degree is cut in realization, improves line Property amplification simple degree.

3, novel tangential flow chromatography device provided by the utility model, Linear Amplifer easy to implement, industrialization easy to implement Production；Can the processing of large capacity sample be realized by increasing the quantity of membrane chromatography material bodies and supporter in the longitudinal direction, In the case of height-limited, the place of large capacity sample can also be realized by amplifying shell, supporter equal-specification in the horizontal Reason.

Description of the drawings

In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model Some embodiments for those of ordinary skill in the art without having to pay creative labor, can also basis These attached drawings obtain other attached drawings.

Fig. 1 is the decomposed structural schematic diagram of novel tangential flow chromatography device in the utility model；

Fig. 2 is the structural schematic diagram of membrane chromatography material bodies in the utility model；

Fig. 3 is the structural schematic diagram of supporter in the utility model；

Fig. 4 is the structural schematic diagram of water distributor in the utility model；

Fig. 5 is the structural schematic diagram of collector pipe in the utility model；

Fig. 6 is that injection port is set to the structural schematic diagram at housing bottom center in the utility model；

Fig. 7 is the structural schematic diagram that injection port is set to housing bottom edge in the utility model；

In figure：1- shells, 2- head covers, 3- membrane chromatographies material bodies, 4- supporters, 5- fluid passages, 6- injection ports, 7- go out sample Mouth, 8- water distributors, 9- cloth sink, 10- water distributing pores, 11- collector pipes, 12- catch basins, 13- gully-holes, 14- ribs, 15- are close Seal.

Specific implementation mode

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work The every other embodiment obtained, shall fall within the protection scope of the present invention.

The purpose of this utility model is to provide a kind of novel tangential flow chromatography device, to solve of the existing technology ask Topic.

To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below in conjunction with the accompanying drawings and have Body embodiment is described in further detail the utility model.

The present embodiment provides a kind of novel tangential flow chromatography devices, as shown in figs. 1-7, including shell 1, head cover 2, membrane chromatography Material bodies 3 and supporter 4；Head cover 2 is set to 1 top of shell, and membrane chromatography material bodies 3 and supporter 4 are set to the interior of shell 1 Portion, between each two membrane chromatography material bodies 3 fitting setting be provided with helical fluid access on supporter 4 there are one supporter 4 5, the initiating terminal of fluid passage 5 is connected to the injection port 6 of 1 bottom of shell, end and the outlet 7 on head cover 2 of fluid passage 5 Connection.

Novel tangential flow chromatography device in the present embodiment further includes a water distributor 8 and a collector pipe 11,8 bottom of water distributor End is connected to injection port 6, and 8 top of water distributor is connect with head cover 2, specifically, an inserting groove is arranged in the bottom of head cover 2, by cloth The top of water pipe 8, which is plugged into inserting groove, to be fixed；In order to realize that water distributor 8 is connect with the fluid passage 5 of each layer, in water distribution It is vertically arranged with a cloth sink 9 on the tube body of pipe 8, concentric water distribution is provided at the center of membrane chromatography material and supporter 4 Hole 10, each layer fluid access 5 connection of cloth sink 9 across water distributing pore 10 and corresponding thereto, the position that water distributing pore 10 is arranged is The initiating terminal of fluid passage 5；11 bottom end of collector pipe is connect with 1 bottom of shell, and 11 top of collector pipe is connected to outlet 7, is catchmented Longitudinally offer a catch basin 12 on the tube body of pipe 11, the edge of membrane chromatography material bodies 3 and supporter 4 is provided with concentric Gully-hole 13, catch basin 12 pass through gully-hole 13 and are connected to fluid passage 5, are fluid passage 5 at the setting of gully-hole 13 End, treated sample is by the end of fluid passage 5 by 12 discharger of catch basin on collector pipe 11；

In the present embodiment, for the installation position of injection port 6, injection port 6 can be set at the center of 1 bottom of shell (referring to attached drawing 6), such installation position when smaller suitable for device volume, should not process the feelings of sample intake passage in 1 bottom of shell Condition；Injection port 6 can also be arranged in the edge (referring to attached drawing 7) of 1 bottom of shell, at this point, the installation position of injection port 6 is applicable in In large volume of device, the injection port 6 and water distributor 8 that a sample intake passage is connected to edge are processed in shell 1.

In order to ensure the stability of supporter 4, in the present embodiment, multiple ribs 14 are additionally provided on supporter 4, it is multiple Ribs 14 are set to 5 bottom of fluid passage, and multiple ribs 14 are circumferentially distributed centered on water distributing pore 10.

In the present embodiment, shell 1 is a barrel-like structure, and head cover 2 is a disc-shaped structure, and head cover 2 passes through bolt with shell 1 It is fixedly connected；Specifically, be integrally formed a flange plate structure at the top of shell 1, circumferentially disposed multiple bolts hole on it, and The corresponding position of head cover 2 is also provided with bolt hole, fixes the two finally by bolt.It is close between head cover 2 and shell 1 in order to ensure Feng Xing also sets up a sealing ring 15 between shell 1 and head cover 2.

In addition, in the present embodiment, membrane chromatography material bodies 3 and supporter 4 are disc-shaped structure, membrane chromatography material bodies 3 with The diameter of supporter 4 is equal with the internal diameter of shell 1.

Above-mentioned novel tangential flow chromatography device provided in this embodiment, during carrying out sample separating treatment, sample warp It crosses injection port 6 and enters water distributor 8, water distributor 8 passes through cloth sink 9 thereon by the fluid passage 5 of sample delivery to each layer, sample By the initiating terminal helical flow of fluid passage 5 to the end of fluid passage 5, during this, due to fluid passage 5 top and Bottom is bonded the membrane chromatography material bodies 3 of setting, thus, sample is tangent with membrane chromatography material bodies 3 in flow process, realizes object The separation of matter；Treated, and sample flows into collector pipe 11 by 5 end of fluid passage via catch basin 12, concentrates defeated by collector pipe 11 It is sent at outlet 7, and then is discharged.

The utility model applies specific case and is expounded to the principles of the present invention and embodiment, the above reality The explanation for applying example is merely used to help understand the method and its core concept of the utility model；Meanwhile for the general of this field Technical staff, according to the thought of the utility model, there will be changes in the specific implementation manner and application range.To sum up, The content of the present specification should not be construed as a limitation of the present invention.

Claims (9)

1. a kind of novel tangential flow chromatography device, it is characterised in that：Including shell, head cover, membrane chromatography material bodies and supporter；Institute It states head cover and is set to the case top, the membrane chromatography material bodies and the supporter are set to the inside of the shell, often It is bonded the supporter there are one being arranged between two membrane chromatography material bodies, helical fluid is provided on the supporter The initiating terminal of access, the fluid passage is communicated with injection port, and the injection port is set to the housing bottom, and the fluid is logical The end on road is communicated with outlet, and the outlet is set on the head cover.

2. novel tangential flow chromatography device according to claim 1, it is characterised in that：Further include a water distributor, the cloth Water pipe bottom end is connected to the injection port, and the water distributor top is connect with the head cover, longitudinal on the tube body of the water distributor It is provided with cloth sink, concentric water distributing pore, the water distribution is provided at the center of the membrane chromatography material and the supporter Slot passes through the water distributing pore and is connected to the fluid passage, is the initiating terminal of the fluid passage at the water distributing pore.

3. novel tangential flow chromatography device according to claim 2, it is characterised in that：The injection port is set to the shell At the center of body bottom.

4. novel tangential flow chromatography device according to claim 2, it is characterised in that：The injection port is set to the shell The edge of body bottom.

5. novel tangential flow chromatography device according to claim 1, it is characterised in that：Further include a collector pipe, the collection Water pipe bottom end is connect with the housing bottom, and the collector pipe top is connected to the outlet, on the tube body of the collector pipe It is vertically arranged with catch basin, the edge of the membrane chromatography material bodies and the supporter is provided with concentric gully-hole, institute Catch basin is stated across the gully-hole and is connected to the fluid passage, is the end of the fluid passage at the gully-hole End.

6. novel tangential flow chromatography device according to claim 1, it is characterised in that：It is additionally provided on the supporter more A ribs, multiple ribs are set to the fluid passage bottom.

7. novel tangential flow chromatography device according to claim 1, it is characterised in that：The shell is a barrel-like structure, The head cover is a disc-shaped structure, and the head cover is bolted to connection with the shell.

8. novel tangential flow chromatography device according to claim 7, it is characterised in that：The case top and the head cover Between be additionally provided with a sealing ring.

9. novel tangential flow chromatography device according to claim 8, it is characterised in that：The membrane chromatography material bodies with it is described Supporter is disc-shaped structure, and the membrane chromatography material bodies are equal with the internal diameter of the shell with the diameter of the supporter.