CN207937680U

CN207937680U - A kind of STED microscopes using negative filter

Info

Publication number: CN207937680U
Application number: CN201820421895.XU
Authority: CN
Inventors: 颜见锋
Original assignee: Suzhou Wei Jia Optical Co Ltd
Current assignee: Suzhou Wei Jia Optical Co Ltd
Priority date: 2018-03-27
Filing date: 2018-03-27
Publication date: 2018-10-02
Anticipated expiration: 2028-03-27

Abstract

The utility model discloses a kind of STED microscopes using negative filter, including light source, negative filter, receiver and the object irradiated by laser, the negative filter is arranged between light source and the object irradiated by laser, the direction of illumination of the negative filter and light source is arranged at 45 degree of angles, the receiver on the right side of negative filter.A kind of STED microscopes using negative filter provided by the utility model, the laser for solving two beam different wave lengths can while irradiate " photographed object ", and the fluorescence that photographed object is excited can be reflected on receiver on same photoelectric cell and switch to photosignal, it ensures the performance and using effect of the instrument, while reducing the cost of entire instrument.

Description

A kind of STED microscopes using negative filter

Technical field

The utility model is related to microscope technology field, more particularly to a kind of STED microscopes using negative filter.

Background technology

The setting of existing SETD microscopes is there are two spectroscope (the first spectroscope and the second spectroscope), two light sources (the One light source and second light source), Figure of description 1 is please referred to, operation principle is as follows：

The laser of wavelength 592nm is penetrated to come from top, it passes through " spectroscope one " of 45 degree of placements, first passes through to 592nm waves " the AR films " of anti-reflection effect is grown, later by 45 degree incident " long wave leads to filter coating ", since the filter coating is to 592nm wavelength High saturating, just directly irradiate onto illuminated object.It may be noted that the high-reflection region of this " long wave leads to filter coating " exists：450nm~ 550nm wave-length coverages.

The laser of another Shu Bochang 488nm can only come in from side, " spectroscopes two " of 45 degree of placements be first passed through, from right 488nm wavelength plays anti-reflection AR films and penetrates, then by 45 degree incident " short-pass filter coatings ", the filter coating is to 488nm Wavelength is also high saturating, but its high-reflection region exists：In 500nm~600nm wave-length coverages.The laser of this Shu Bochang 488nm is just It is mapped on " long wave leads to filter coating " of " spectroscope one ", since " long wave leads to filter coating " is in wavelength 450nm to 550nm wavelength models It is high reflection to enclose, so being just reflected on radiation exposed object, that is to say, that：It is illuminated object picture and uses " negative filter " The same irradiation for also receiving two kinds of optical maser wavelengths (488nm and 592nm).Be illuminated " fluorescence " that object is inspired using " spectroscope one " is reflected into " receiver " with " spectroscope two ".Obviously, light path or even its instrument can be complicated, manufacture efficiency It is all very high with cost.

Utility model content

For the setting of above-mentioned existing SETD microscopes there are two spectroscope, two light sources, light path or even its instrument Structure is all extremely complex, and all very high problem of manufacture efficiency and cost, the technical problems to be solved in the utility model is caused to be A kind of STED microscopes using negative filter are provided.

In order to solve the above-mentioned technical problem, the technical solution adopted in the utility model is：

A kind of STED microscopes using negative filter, including light source, negative filter, receiver and the object irradiated by laser Body, the negative filter are arranged between light source and the object irradiated by laser, the direction of illumination of the negative filter and light source At 45 degree of angles, the receiver is arranged on the right side of negative filter.

The preferred embodiment of said program is：The negative filter includes optical glass, AR films and negative filtering film, the optics Glass side is provided with AR films, the other side is provided with negative filtering film.

The preferred embodiment of said program is：Total 32 layers of the negative filtering film, by TiO₂And SiO₂Alternately form.

The preferred embodiment of said program is：The light source is by the combination of light sources of two different wavelength at wavelength difference For 488nm and 592nm.

Advantageous effect：A kind of STED microscopes using negative filter of the utility model, wavelength 488nm's and 592nm The AR films on negative filter that two beam laser are placed by 45 degree first, followed by passing through, " negative filtering film due to AR films and bears filter Light film is high saturating to the two wavelength, and after irradiating onto illuminated object, the wavelength inspired projects again for the fluorescence of 525nm Onto negative filtering film, and negative filtering film is high reflection to the light of 525nm, is just reflected on receiver and switchs to electric signal, to It is shown on instrument, the negative filtering film of 45 degree of incidence makes entire instrument reduce cost.It is provided by the utility model A kind of STED microscopes using negative filter, the laser for solving two beam different wave lengths can carry out spoke to " photographed object " simultaneously According to, and the fluorescence that photographed object is excited can be reflected on receiver on same photoelectric cell and switch to photosignal, it is ensured that it should The performance and using effect of instrument, while reducing the cost of entire instrument.

Description of the drawings

Fig. 1 is existing STED microscopic structures schematic diagram；

Fig. 2 is a kind of microscopical structural schematic diagrams of STED using negative filter of the utility model.

Fig. 3 is the structural schematic diagram of negative filter in the utility model.

In figure：1- light sources, 2- negative filters, the object that 3- is irradiated by laser, 4- receivers, 5- first light sources, 6- second light sources, the first spectroscopes of 7-, the second spectroscopes of 8-, 9-AR films, 10- optical glass, 11- negative filtering films.

Specific implementation mode

To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below in conjunction with the accompanying drawings to this The specific implementation mode of utility model is described in detail.Elaborate many details in order to abundant in the following description Understand the utility model.But the utility model can be much to implement different from other manner described here, this field Technical staff can do similar improvement without prejudice to the utility model connotation, therefore the utility model is not by following public affairs The limitation for the specific embodiment opened.

It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to To another element or it may be simultaneously present centering elements.

Unless otherwise defined, all of technologies and scientific terms used here by the article is led with the technology for belonging to the utility model The normally understood meaning of technical staff in domain is identical.Terminology used in the description of the utility model herein only be The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes Any and all combinations of one or more relevant Listed Items.

As shown in Figure 2,3, the utility model provides a kind of STED microscopes using negative filter, including light source 1, negative Optical filter 2, receiver 4 and the object 3 irradiated by laser, the negative filter 2 are arranged in light source 1 and the object irradiated by laser Between 3, at 45 degree of angles, receiver 4 is arranged on the right side of negative filter 2 direction of illumination of negative filter 2 and light source 1.

Wherein, negative filter 2 includes optical glass 10, AR films 9 and negative filtering film 11, and 10 side of optical glass is provided with AR films 9, the other side are provided with negative filtering film 11.

Wherein, 11 total 32 layers of negative filtering film, by TiO₂And SiO₂Alternately form.

Wherein, light source 1 by the combination of light sources of two different wavelength at wavelength is respectively 488nm and 592nm.

From the above mentioned, the utility model has reached following technique effect：The laser for solving two beam different wave lengths can be simultaneously " photographed object " is irradiated, and the fluorescence that photographed object is excited can be reflected on receiver on same photoelectric cell and switch to light Electric signal, it is ensured that the performance and using effect of the instrument, while reducing the cost of entire instrument.

Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, it is all considered to be the range of this specification record.

Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed, But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.

Claims

1. a kind of STED microscopes using negative filter, which is characterized in that including light source, negative filter, receiver and swashed The object of light irradiation, the negative filter are arranged between light source and the object irradiated by laser, the negative filter and light source Direction of illumination at 45 degree of angles, the receiver is arranged on the right side of negative filter.

2. the STED microscopes according to claim 1 using negative filter, the negative filter includes optical glass, AR Film and negative filtering film, the optical glass side is provided with AR films, the other side is provided with negative filtering film.

3. the STED microscopes according to claim 2 using negative filter, which is characterized in that the negative filtering film total 32 Layer, by TiO₂And SiO₂Alternately form.

4. the STED microscopes according to claim 1 using negative filter, which is characterized in that the light source by two kinds not The combination of light sources of same wavelength is at wavelength is respectively 488nm and 592nm.