CN207933321U - A kind of super filter tube for protein isolate matter - Google Patents

A kind of super filter tube for protein isolate matter Download PDF

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Publication number
CN207933321U
CN207933321U CN201820217509.5U CN201820217509U CN207933321U CN 207933321 U CN207933321 U CN 207933321U CN 201820217509 U CN201820217509 U CN 201820217509U CN 207933321 U CN207933321 U CN 207933321U
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China
Prior art keywords
filter
column
filter column
protein isolate
tube
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CN201820217509.5U
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Chinese (zh)
Inventor
郝星凯
吴浩
周敏
孙美娜
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Greentown Agricultural Detection Technology Co Ltd
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Greentown Agricultural Detection Technology Co Ltd
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Abstract

The utility model discloses a kind of super filter tubes for protein isolate matter, more particularly to a kind of Laboratory Instruments, including filter device and hollow and one end open tube body, the filter device is set to the hollow inside of tube body, and the filter device includes the first Filter column, the receiving pipe for accommodating filtered protein and fixing device;First Filter column includes the first cylinder of both ends open, and one end of first cylinder is equipped with the first filter membrane;The fixing device include the fixation slotting ring being arranged on the first cylinder and be arranged accommodate pipe on the first caulking groove;The fixed slotting ring is equipped with limited block, and correspondingly, the first caulking groove slot bottom is transversely provided with the annular fixing groove of adaptation limited block;The pipe that accommodates is equipped with the first sliding groove that the first caulking groove slot bottom is slid into for limited block.The utility model has the advantages that easy to operate and experimental efficiency is high.

Description

A kind of super filter tube for protein isolate matter
Technical field
The utility model is related to a kind of Laboratory Instruments, specially a kind of super filter tube for protein isolate matter.
Background technology
Ultrafiltration centrifugation technique has obtained very in bioscience, especially biochemistry and molecular biology research field It is widely applied, each biochemistry and Molecular Biology Lab will prepare the centrifuge of a variety of patterns.Ultrafiltration centrifuges skill Art is mainly used for the separation and preparation of various biological samples, and biological sample suspension is contained in centrifuge tube under high speed rotation, Due to huge centrifugation force effect, make the molecule of suspension, such as the precipitation of cell, large biological molecule is saturating with certain speed The ultrafiltration membrane with certain pore size is crossed, to enable macromolecular to be detached with small molecule, centrifuge tube and centrifuge match, and use In this centrifugation technique.It is bothered very much however, existing centrifugal ultrafiltration pipe uses, leads to the working efficiency of experimenter very It is low.
Utility model content
The utility model provides a kind of super filter tube for protein isolate matter, which has easy to operate and real Test efficient advantage.
To achieve the above object, the utility model provides the following technical solutions:
A kind of super filter tube for protein isolate matter, including filter device and hollow and one end open tube body, it is described Filter device is set to the hollow inside of tube body, and the filter device includes the first Filter column, for accommodating filtered albumen The receiving pipe and fixing device of matter;First Filter column includes the first cylinder of both ends open, and the one of first cylinder End is equipped with the first filter membrane;The fixing device includes the fixation slotting ring being arranged on the first cylinder and setting on accommodating pipe The first caulking groove;The fixed slotting ring is equipped with limited block, and correspondingly, the first caulking groove slot bottom is transversely provided with adaptation limit The annular fixing groove of position block;The pipe that accommodates is equipped with the first sliding groove that the first caulking groove slot bottom is slid into for limited block.
Wherein, filter device can be used for filtration protein solution, and protein solution after filtering, is all collected and accommodating pipe It is interior.Also, experimenter can again be centrifuged, you can obtain target molecule by replacing the filter membrane of different pore size again The protein of amount.Aforesaid operations are very simple, and after the completion of operation, and protein solution is present in receiving pipe either the first column On body, the cleaning of lab assistant is also allowed for.
Preferably, the filter device further includes the second Filter column and third Filter column, second Filter column and It is detachably fixed by the fixing device between one Filter column, passes through institute between the third Filter column and the second Filter column The fixing device stated is detachably fixed, detachably solid by the fixing device between the third Filter column and receiving pipe It is fixed.
Preferably, second Filter column is equipped with the second filter membrane, the aperture of second filter membrane is less than the first mistake The aperture of filter membrane.
Preferably, the third Filter column is equipped with third filter membrane, the aperture of the third filter membrane is less than the second mistake The aperture of filter membrane.
Preferably, inboard wall of tube body is equipped with locating slot, correspondingly, first Filter column is equipped with the positioning of adaptation locating slot Block.
Preferably, body open ends are equipped with lid, the tube body lateral wall is sheathed on retaining collar, the fixing sleeve Ring is fixedly connected with lid by rope.
The beneficial effects of the utility model are:
In the present invention, filter device can be used for filtration protein solution, and protein solution after filtering, is all received Collection is accommodating in pipe.Also, experimenter can again be centrifuged by replacing the filter membrane of different pore size again, you can To the protein of target molecular weight.Aforesaid operations are very simple, and operate after the completion of, protein solution be present in receiving pipe or Person is to also allow for the cleaning of lab assistant on the first cylinder.
Description of the drawings
Fig. 1 is the structural schematic diagram of filter device in the present embodiment;
Fig. 2 is the cross-sectional view of filter device in the present embodiment;
Fig. 3 is the structural schematic diagram of the present embodiment middle tube body.
In figure:1, pipe is accommodated, the 12, first caulking groove, 13, annular fixing groove, 2, third Filter column, 25, third filter membrane, 3, Second Filter column, the 35, second filter membrane, the 4, first Filter column, 41, locating piece, the 45, first filter membrane, 5, tube body, 51, lid, 52, locating slot, 53, retaining collar.
Specific implementation mode
The present embodiment provides a kind of technical solutions:
As shown in Figures 1 to 3, a kind of super filter tube for protein isolate matter, including filter device and hollow and one end are opened The tube body 5 of mouth, the filter device are set to the hollow inside of tube body 5, and the filter device includes the first Filter column 4, is used for Accommodate the receiving pipe 1 and fixing device of filtered protein;First Filter column 4 includes the first column of both ends open One end of body, first cylinder is equipped with the first filter membrane 45;The fixing device includes the fixation being arranged on the first cylinder Slotting ring 42 and the first caulking groove 12 being arranged on accommodating pipe 1;The fixed slotting ring 42 is equipped with limited block 41, correspondingly, institute State the annular fixing groove 13 that 12 slot bottom of the first caulking groove is transversely provided with adaptation limited block 41;The receiving pipe 1 is equipped with for limit Block 41 slides into the first sliding groove 11 of 12 slot bottom of the first caulking groove.
In the present embodiment, filter device can be used for filtration protein solution, and protein solution after filtering, is all collected It is accommodating in pipe.Also, experimenter can again be centrifuged, you can obtain by replacing the filter membrane of different pore size again The protein of target molecular weight.Aforesaid operations are very simple, and operate after the completion of, protein solution be present in receiving pipe or It is to also allow for the cleaning of lab assistant on the first cylinder.
As shown in Figure 1, the filter device further includes the second Filter column 3 and third Filter column 2, second Filter column 3 Be detachably fixed by the fixing device between the first Filter column 4,2 and second Filter column 3 of the third Filter column it Between be detachably fixed by the fixing device, the third Filter column 2 and accommodate pipe 1 between pass through the fixing device It is detachably fixed.
In the present embodiment, second Filter column 3 is equipped with the second filter membrane 35, and the aperture of second filter membrane 35 is small In the aperture of the first filter membrane 45.
In the present embodiment, the third Filter column 2 is equipped with third filter membrane 25, and the aperture of the third filter membrane 25 is small In the aperture of the second filter membrane 35.
In the present embodiment, first filter membrane 45 be protein for molecular weight less than 10KD by the first half Permeable membrane.
In the present embodiment, second Filter column 3 is equipped with the second filter membrane 35, and second filter membrane 35 is for dividing Son amount less than 8KD protein by the second semi-permeable membrane.
In the present embodiment, the third Filter column 2 is equipped with third filter membrane 25, and the third filter membrane 25 is for dividing Son amount less than 6KD protein by third semi-permeable membrane.
As shown in figure 3,5 inner wall of tube body is equipped with locating slot 52, correspondingly, first Filter column 4 is equipped with adaptation locating slot 52 locating piece 41.
As shown in figure 3,5 open end of tube body is equipped with lid 51,5 lateral wall of the tube body is sheathed on retaining collar 53, institute Retaining collar 53 is stated to be fixedly connected by rope with lid 51.
Application method:It is respectively for molecular weight with third filter membrane 25, the second filter membrane 35 and the first filter membrane 45 Less than 6KD, molecular weight be less than the protein of 8KD and molecular weight less than 10KD by semi-permeable membrane for, be discussed below specific Application method:
Protein solution is poured into the first Filter column 4, while a sealing cover can be used and cover opening for the first Filter column 4 At mouthful, avoids protein solution from being thrown away in centrifugal process and come.After the completion of centrifugation, the protein stayed in the first Filter column 4 is molten Liquid is exactly the protein that molecular weight is more than 10KD, and the protein solution stayed in the second Filter column 3 is exactly that molecular weight is in 8KD extremely Protein between 10KD, the protein solution stayed in third Filter column 2 are exactly that molecular weight is in 6KD to the egg between 8KD White matter, it is exactly the protein that molecular weight is less than 6KD to stay in and accommodate the protein solution in pipe 1, and experimenter can be according to reality Separation is needed to obtain the protein of corresponding molecular level, without separating for several times, to substantially increase conventional efficient.
Wherein, when lab assistant is wanted to obtain the protein of other molecular levels, the mistake on replaceable corresponding Filter column Filter membrane, or the Filter column for installing corresponding semi-permeable membrane is directly replaced, to easily facilitate the operation of lab assistant.
Although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art is come Say, still can with technical scheme described in the above embodiments is modified, or to which part technical characteristic into Row equivalent replacement, within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on should all It is included within the scope of protection of this utility model.

Claims (6)

1. a kind of super filter tube for protein isolate matter, including filter device and hollow and one end open tube body (5), described Filter device is set to the hollow inside of tube body (5), which is characterized in that the filter device includes the first Filter column (4), is used for Accommodate the receiving pipe (1) and fixing device of filtered protein;
First Filter column (4) includes the first cylinder of both ends open, and one end of first cylinder is equipped with the first filter membrane (45);
The fixing device includes first of the fixation slotting ring (42) being arranged on the first cylinder and setting on accommodating pipe (1) Caulking groove (12);
The fixed slotting ring (42) is equipped with limited block (41), and correspondingly, the first caulking groove (12) slot bottom is transversely provided with adaptation The annular fixing groove (13) of limited block (41);
The pipe (1) that accommodates is equipped with the first sliding groove (11) that the first caulking groove (12) slot bottom is slid into for limited block (41).
2. a kind of super filter tube for protein isolate matter according to claim 1, which is characterized in that the filter device is also Including the second Filter column (3) and third Filter column (2), by described between second Filter column (3) and the first Filter column (4) Fixing device be detachably fixed, can by the fixing device between the third Filter column (2) and the second Filter column (3) Dismounting is fixed, and is detachably fixed by the fixing device between the third Filter column (2) and receiving pipe (1).
3. a kind of super filter tube for protein isolate matter according to claim 2, which is characterized in that second Filter column (3) it is equipped with the second filter membrane (35), the aperture of second filter membrane (35) is less than the aperture of the first filter membrane (45).
4. a kind of super filter tube for protein isolate matter according to claim 3, which is characterized in that the third Filter column (2) it is equipped with third filter membrane (25), the aperture of the third filter membrane (25) is less than the aperture of the second filter membrane (35).
5. a kind of super filter tube for protein isolate matter according to claim 1, which is characterized in that tube body (5) inner wall is set There is locating slot (52), correspondingly, first Filter column (4) is equipped with the locating piece (41) of adaptation locating slot (52).
6. a kind of super filter tube for protein isolate matter according to claim 1, which is characterized in that tube body (5) open end It is equipped with lid (51), tube body (5) lateral wall is sheathed on retaining collar (53), the retaining collar (53) and lid (51) It is fixedly connected by rope.
CN201820217509.5U 2018-02-07 2018-02-07 A kind of super filter tube for protein isolate matter Active CN207933321U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820217509.5U CN207933321U (en) 2018-02-07 2018-02-07 A kind of super filter tube for protein isolate matter

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820217509.5U CN207933321U (en) 2018-02-07 2018-02-07 A kind of super filter tube for protein isolate matter

Publications (1)

Publication Number Publication Date
CN207933321U true CN207933321U (en) 2018-10-02

Family

ID=63651908

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820217509.5U Active CN207933321U (en) 2018-02-07 2018-02-07 A kind of super filter tube for protein isolate matter

Country Status (1)

Country Link
CN (1) CN207933321U (en)

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