CN207871213U - A kind of circulating tumor cell capturing device - Google Patents

Abstract

The utility model discloses a kind of circulating tumor cell capturing devices, including one or more containers for handling collection circulating tumor cell, container inner wall has been fitted into the antibody that can be specifically bound with circulating tumor cell, or has been fitted into surface modification and has the nano particle of antibody；Container is screw type container or the straight barrel type containing helicitic texture, can make the liquid inside inflow container that spiral advance be presented, and then generate centrifugal force；Circulating tumor cell can generate the centrifugal force of bigger relative to red blood cell, blood platelet, and then be specifically bound with the antibody on chamber wall.Two kinds of principles are combined, and can not only realize the capture to whole body CTC, reduce the probability of metastases；The CTC being enriched on container inner wall can be collected simultaneously, so as to its further characterization and research.

Description

A kind of circulating tumor cell capturing device

Technical field

The utility model is related to a kind of circulating tumor cell capturing devices, belong to medicine instrument technical field.

Background technology

After tumour is the disease for being difficult to capture in present illness, and tumour shifts, the survival rate of patient is even more big It is big to reduce.The process of metastases is extremely complex, to the circulating tumor cell in blood（CTC）, miRNA, excretion body detection With certain reference value.Pioneer officials of the CTC as transfer plays the role of tumour generation transfer vital.

Have at present based on cell size and surface antibody principle, the CTC numbers in a small amount of blood of vitro detection, to patient's Certain reference value is played in treatment and prognosis.But since vitro detection volume is less, the tumour cell of whole body cannot be carried out Detection, is susceptible to error, needs repeatedly to measure, and cannot play therapeutic effect.

In order to remove the circulating tumor cell in blood samples of patients comprehensively, prevents tumour from far-end transfer occurs, need research and development one Kind circulating tumor cell capturing device.

Utility model content

Purpose：In order to overcome the deficiencies in the prior art, the utility model to provide a kind of circulating tumor cell capture The blood of patient can be drawn extracorporeal circulation apparatus, after capturing circulating tumor cell, the blood of purification is conveyed the Huis by device Body blood circulation, can replace/existing chemotherapy or radiotherapy clinically is assisted, it is general to effectively reduce tumour far-end transfer Rate reduces the injury to patient, improves patient's cure rate.Meanwhile, it is capable to circulating tumor cell CTC is collected into from chamber wall, Guiding significance is all had to the diagnosis and treatment of patient.

Technical solution：In order to solve the above technical problems, the technical solution adopted in the utility model is：

A kind of circulating tumor cell capturing device, it is characterised in that：It is thin that collection circulating tumor is handled including one or more The container of born of the same parents,

The container inner wall has been fitted into the antibody that can be specifically bound with circulating tumor cell, or has been fitted into table Face is modified with the nano particle of the antibody；The antibody is the specific antibody of circulating tumor cell CTC, and preferably anti-epithelium is thin Born of the same parents' attachment proteins（EpCAM）, cytokeratin (cytokeratin, CK), vascular endothelial growth factor（vascular endothelial growth factor, VEGF）, ErbB1（epidermal growth factor receptor, EGFR）, proto-oncogene ErbB-2（human epidermal growth factor receptor-2, HER2）One or more of antibody；

The container is screw type container or the straight barrel type containing helicitic texture, can make the liquid inside inflow container be in It is now spiral to advance, and then generate centrifugal force；Circulating tumor cell can generate the centrifugation of bigger relative to red blood cell, blood platelet Power, and then specifically bound with the antibody on chamber wall.

Preferably, the circulating tumor cell capturing device, it is characterised in that：The container inner wall is carved with recessed The screw thread of swaged or male-type.

As another preferred embodiment, the circulating tumor cell capturing device, it is characterised in that：The container is straight tube Type container, helicitic texture be inner wall be carved with the screw thread of umbilicate type or male-type or in the container of straight barrel type place there are one or More than one screw rod or flight.

Preferably, the circulating tumor cell capturing device, it is characterised in that：The nano particle is received for magnetic Rice grain, gold nano grain, nano SiO 2 particle, metal organic framework nano particle, polymer nano granules, albumen are received One or more of rice grain, liposome.

Preferably, the circulating tumor cell capturing device, it is characterised in that：Contain on the nano particle The connecting key of response fracture.

Further, the circulating tumor cell capturing device, it is characterised in that：The company of the response response fracture The response for connecing key generation is redox response, pH responses or photosensitivity response.

The utility model also provides a kind of circulating tumor cell dialysis treatment instrument, including arterial pressure table, blood pump, heparin Pump, feed pressure table, off gas system, vein pressure table further include the above-mentioned circulating tumor cell capture dress of claim 1 to 6 It sets, arterial pressure table, blood pump, heparin pump, feed pressure table, circulating tumor cell capturing device, off gas system, vein pressure Table is sequentially connected by extracorporeal blood pipeline；Blood pump provides the power of extracorporeal circulation of blood；Heparin pump provides heparin to blood, Prevent blood coagulation；Circulating tumor cell capturing device is for capturing circulating tumor cell；The blood of purification passes through off gas system After excluding bubble, patient's body is transported to after vein pressure table detects blood qualification.

More specifically, the circulating tumor cell dialysis treatment instrument, it is characterised in that：The off gas system includes deflating Valve and air monitor.

Advantageous effect：Circulating tumor cell capturing device provided by the utility model contains helicitic texture using inner wall Straight barrel type or screw type container, or the container containing screw rod or flight make the out-of-date generation centrifugation that can be vortexed of blood stream Power, antigen-antibody are specifically bound；Two kinds of principles are combined, and can not only realize the capture to whole body CTC, reduce tumour The probability of transfer；The CTC being enriched on container inner wall can be collected simultaneously, so that it is further characterized and ground Study carefully.It can be good at inhibiting the diffusion of tumour cell in vivo, can replace/assist existing chemotherapy or radiation clinically Therapy effectively reduces tumour far-end transfer probability, reduces the injury to patient, improves patient's cure rate.Meanwhile, it is capable to from container It is collected into CTC on wall, guiding significance is all had to the diagnosis and treatment of patient.

Description of the drawings

Fig. 1 is the structural schematic diagram of circulating tumor cell dialysis treatment instrument（Arrow indicates blood flow direction）；

Fig. 2 is the schematic diagram of screw type container circulating tumor cell capturing device;

Fig. 3 is the schematic diagram that inner wall carves threaded straight barrel type container circulating tumor cell capturing device；

Fig. 4 is the schematic diagram for the circulating tumor cell capturing device that straight barrel type container is embedded in screw rod;

Fig. 5 is the schematic diagram for the circulating tumor cell capturing device that straight barrel type container is embedded in flight;

Fig. 6 is that blood flow advances in the container inner wall inside spin containing helicitic texture, and specificity chimeric with its surface CTC is anti- The schematic diagram that body combines；

Figure label：1 arterial pressure table, 2 blood pumps, 3 heparin pumps, 4 feed pressure tables, 5 circulating tumor cells capture dress It sets, 6 off gas systems, 7 vein pressure tables.

Specific implementation mode

The utility model is further described with reference to specific embodiment.

As shown in Figures 2 to 5, a kind of circulating tumor cell capturing device, including one or more processing collect cycle and swell The container of oncocyte, the container inner wall have been fitted into the antibody that can be specifically bound with circulating tumor cell, Huo Zheqian Having closed surface modification has the nano particle of the antibody.The antibody is anti-Epithelial Cell Adhesion albumen（EpCAM）, cytokeratin (cytokeratin, CK), vascular endothelial growth factor（vascular endothelial growth factor, VEGF）、 ErbB1（epidermal growth factor receptor, EGFR）, proto-oncogene human epidermal life Growth factor receptor body 2（human epidermal growth factor receptor-2, HER2）One kind or several in antibody Kind.

The nano particle is magnetic nano particle, gold nano grain, nano SiO 2 particle, metal organic framework （MOF）One or more of nano particle, polymer nano granules, protein nano particle, liposome.Further, described Containing on nano particle can be broken with response（Redox response, pH responses, photosensitivity response etc.）Connecting key.

Container can be the container of screw type container or straight barrel type；When for screw type container, container inner wall is carved with recess The screw thread of type or male-type, as shown in Figure 2；Screw type container itself can also be passed through without umbilicate type or the screw thread of male-type Helical structure can also realize and make liquid inside inflow container that spiral advance be presented.

Another scheme, as shown in figure 3, when for the container of straight barrel type, container inner wall is carved with umbilicate type or male-type Screw thread.

Another preferred embodiment when for the container of straight barrel type, is placed in the container of straight barrel type as shown in Figure 4 and Figure 5 Appropriately sized screw rod or flight.

Before the circulating tumor cell capturing device of the utility model can be such that the liquid inside inflow container presents spirally Into, and then generate centrifugal force；The larger circulating tumor cell of individual can generate the centrifugation of bigger relative to red blood cell, blood platelet Power, and then specifically bound with the antibody on chamber wall.

As shown in Figure 1, a kind of circulating tumor cell dialysis treatment instrument, including arterial pressure table 1, blood pump 2, heparin pump 3, Feed pressure table 4, circulating tumor cell capturing device 5, off gas system（Vent valve and air monitor）6, vein pressure table 7, Arterial pressure table 1, blood pump 2, heparin pump 3, feed pressure table 4, circulating tumor cell capturing device 5, off gas system（Vent valve And air monitor）6, vein pressure table 7 is sequentially connected by extracorporeal blood pipeline, and outside is equipped with casing.Wherein blood pump 2 carries For external sanguimotor power；Heparin pump 3 provides heparin to blood, prevents blood coagulation；Circulating tumor cell capturing device 5 Circulating tumor cell can be captured；The blood of purification detects blood after off gas system 6 excludes bubble, in vein pressure table 7 It is transported to patient's body after liquid qualification.Used unit and connecting pipe are medical grade.

Connecting pipe used in circulating tumor cell dialysis treatment instrument, pressure gauge, blood pump, heparin pump are that medical treatment is common Equipment.Circulating tumor cell capturing device 5 is made of multiple screw types or straight barrel type container, and each container is respectively equipped with blood Inflow entrance and outflux, and the inner wall of each container contains helicitic texture.When blood flows through circulating tumor cell capturing device When, the helicitic texture of container inner wall and（Or）The helical structure of container makes blood advance in container inside spin, and then can be with It is chimeric to there is the chamber wall of CTC specific antibodies to come into full contact with；Simultaneously because CTC volumes are larger, generated relatively under vortex conditions Big centrifugal force, it is easier to which the CTC specific antibody chimeric with container inner wall combines, as shown in fig. 6, in turn by CTC from blood Middle separation.The blood of patient is received by after " dialysis ", CTC is remained on container inner wall after carrying out specially treated to this container Collect CTC cells, in order to the further research to CTC cells.

The utility model draws blood to be clean to capture circulating tumor cell device from human body artery blood vessel, and setting is dynamic Pulse pressure table monitors blood pressure；Heparin pump is flowed to by blood pump, blood coagulation occurs to prevent Hemostatic Oral Liquid；It is swollen subsequently into capture cycle After the core apparatus of oncocyte, using container inner wall helicitic texture and（Or）The helical structure of container, the blood flowed through It is threadingly advanced, and then can be come into full contact with the chimeric container inner wall for having CTC specific antibodies；Simultaneously because CTC volumes are larger, Relatively large centrifugal force is generated under vortex conditions, it is easier to which the CTC specific antibody chimeric with container inner wall combines, and then will CTC is detached from blood；The deaerated system of blood after final purification excludes bubble；Patient is returned to after the detection of vein pressure table In vivo.The blood of patient is collected by after " dialysis ", CTC is remained on container inner wall after carrying out specially treated to this container CTC cells, in order to the further research to CTC cells.The utility model can replace/existing the chemotherapy clinically of auxiliary Or radiotherapy, tumour far-end transfer probability is effectively reduced, patient's cure rate is improved.Meanwhile, it is capable to be collected into from chamber wall CTC all has guiding significance to the diagnosis and treatment of patient.

The above is only the preferred embodiment of the utility model, it should be pointed out that：For the common skill of the art For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and Retouching also should be regarded as the scope of protection of the utility model.

Claims (9)

1. a kind of circulating tumor cell capturing device, it is characterised in that：It is handled including one or more and collects circulating tumor cell Container,

The container inner wall has been fitted into the antibody that can be specifically bound with circulating tumor cell, or has been fitted into surface and has repaiied It is decorated with the nano particle of antibody；The antibody is the specific antibody of circulating tumor cell；

The container is screw type container or the straight barrel type containing helicitic texture, can make the liquid inside inflow container that spiral shell be presented Rotating advance, and then generate centrifugal force；Circulating tumor cell can generate the centrifugal force of bigger relative to red blood cell, blood platelet, And then it is specifically bound with the antibody on chamber wall.

2. circulating tumor cell capturing device according to claim 1, it is characterised in that：The antibody is anti-epithelial cell Attachment proteins, cytokeratin, vascular endothelial growth factor, ErbB1, proto-oncogene human epidermal growth because One kind in 2 antibody of sub- receptor.

3. circulating tumor cell capturing device according to claim 1, it is characterised in that：The container inner wall is carved with recess The screw thread of type or male-type.

4. circulating tumor cell capturing device according to claim 1, it is characterised in that：The container holds for straight barrel type Device is placed with one or more screw rod or flight in the container of straight barrel type.

5. circulating tumor cell capturing device according to claim 1, it is characterised in that：The nano particle is magnetic nanometer Particle, gold nano grain, nano SiO 2 particle, metal organic framework nano particle, polymer nano granules, protein nano One kind in particle, liposome.

6. circulating tumor cell capturing device according to claim 1, it is characterised in that：Contain sound on the nano particle The connecting key of answering property fracture.

7. circulating tumor cell capturing device according to claim 6, it is characterised in that：The response response fracture The response that connecting key generates is redox response, pH responses or photosensitivity response.

8. a kind of circulating tumor cell dialysis treatment instrument, including arterial pressure table, blood pump, heparin pump, feed pressure table, degasification System, vein pressure table, it is characterised in that：Further include claim 1-7 any one of them circulating tumor cell capturing devices, Arterial pressure table, blood pump, heparin pump, feed pressure table, circulating tumor cell capturing device, off gas system, vein pressure table It is sequentially connected by extracorporeal blood pipeline；Blood pump provides the power of extracorporeal circulation of blood；Heparin pump provides heparin to blood, prevents Blood coagulation only occurs；Circulating tumor cell capturing device is for capturing circulating tumor cell.

9. circulating tumor cell dialysis treatment instrument according to claim 8, it is characterised in that：The off gas system includes putting Air valve and air monitor.