CN207803320U - A kind of bacillus subtilis and trichoderma microcapsule granule - Google Patents
A kind of bacillus subtilis and trichoderma microcapsule granule Download PDFInfo
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- CN207803320U CN207803320U CN201820128736.0U CN201820128736U CN207803320U CN 207803320 U CN207803320 U CN 207803320U CN 201820128736 U CN201820128736 U CN 201820128736U CN 207803320 U CN207803320 U CN 207803320U
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Abstract
The utility model discloses a kind of bacillus subtilises and trichoderma microcapsule granule, belong to microorganism microcapsule technology field, the microcapsule granule includes the double-deck core material, the double-deck wall material, the double-deck core material and the double-deck wall material are solid concentric structure, the double-deck core material and the double-deck wall material putting in order as the first core material from the inside to surface, first wall material, second core material, second wall material, first core material is trichoderma, first wall material is saccharomycete biological cell wall, second core material is bacillus subtilis fermentation liquor, second wall material includes maltodextrin layer and gelatin layer from the inside to surface, the particle of preparation has the double effects of bacillus subtilis and trichoderma, and two core material belong to isolation, survival and the accumulation of nutriment can preferably be carried out.
Description
Technical field
The utility model belongs to microorganism microcapsule technology field, and in particular to a kind of bacillus subtilis and trichoderma are micro-
Capsule particle.
Background technology
Bacillus subtilis, which is that a kind of Gram-positive being distributed widely in various different living environments is rod-shaped, supports well type
Bacterium can generate endogenous spore, and heat-resisting resistance is strong, in the surface generally existing of soil and plant, while still plant
A kind of interior common endophyte, it is nontoxic to people and animals, it is free from environmental pollution.Since the bacillus subtilis speed of growth is fast, nutrition
Demand is simple, is easy to survive, colonize and breed, no pathogenicity, and can secrete a variety of enzymes and antibiotic, but also with good
Fermentation basis, purposes is very extensive, and Trichoderma is the widely distributed fungi of nature, have higher economic value and application,
Trichoderma is mainly used for preventing the soil-borne disease of all kinds of plants, and part leaf portion and fringe portion disease.Trichoderma can not only diseases prevention,
Also have and promote plant growth, improve nutritional utilization efficiency, enhance stress resistance of plant and repairs the functions such as agrochemical pollution environment, I
State's trichoderma be mainly used in all kinds of vegetables, forest and Maize Soil Borne Disease and eliminating vegetable botrytis, downy mildew, powdery mildew,
Rice blast and the withered prevention of wheat line, but how to incorporate integrated control technique system in the application or mutually assisted with chemical prevention
Tune lacks further investigation.
Microencapsulation refers generally to utilize the method for physics, chemistry and physical chemistry or biology by chemical substance or life
Object material is coated in cyst material, its slow release is made even to control release.Microencapsulation has following main advantage:1) it protects
Sensitive active ingredient reduces the influence of the environmental factors such as air, light；2) its common state be dry powder or microcapsule suspending agent, it is molten
Agent dosage is relatively fewer, can reduce environmental pressure and reduce the wasting of resources；3) it can significantly reduce to higher mammal, non-target organism
Deng toxicity；4) Release of core material can be delayed, extend the lasting period；5) it can reduce or shelter bad smell etc..Pass through microencapsulation
The multi-functional microcapsule granule with sustained release performance can be prepared very well.
Utility model content
According to the above-mentioned deficiencies of the prior art, the utility model proposes a kind of bacillus subtilises and trichoderma micro-capsule
Grain, in order to solve the above-mentioned technical problem, the technical solution adopted in the utility model is：
A kind of bacillus subtilis and trichoderma microcapsule granule, including the double-deck core material, the double-deck wall material, the double-deck core material and double
Layer wall material is solid concentric structure, the double-deck core material and the double-deck wall material putting in order as the first core material from the inside to surface,
First wall material, the second core material, the second wall material, first core material are trichoderma, and first wall material is given birth to for saccharomycete
Object cell wall, second core material are bacillus subtilis fermentation liquor, and second wall material includes maltodextrin from the inside to surface
Layer and gelatin layer.
Preferably, a diameter of 0.2-0.5mm of the solid concentric circles.
Preferably, the thickness of first wall material is 0.15-0.25 μm.
Preferably, the thickness of the maltodextrin layer is 15-25 μm, and institute's gelatin layer thickness is 10-20 μm.
Preferably, first core material and the second core material also include culture medium, and the amount of the first core material is the second core material
0.3-0.5 times.
Compared with prior art, the utility model has the beneficial effects that：
1. the utility model is had using bacillus subtilis and core material of the trichoderma as microcapsule granule, the particle of preparation
There are the double effects of bacillus subtilis and trichoderma, and two core materials belong to isolation, preferably can be survived and be sought
Support the accumulation of substance.
2. the second wall material improves the stability and integrality of entire particle using the double-deck wall material structure in the utility model,
It prevents particle from leading to the loss of core material due to extruding in transportational process, improves the utilization rate of raw material.
Description of the drawings
In order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, other drawings may also be obtained based on these drawings.
1. Fig. 1 is bacillus subtilis and trichoderma microcapsule granule structural schematic diagram in the utility model embodiment；
1, the first core material, the 2, first wall material, the 3, second core material, the 4, second wall material, 41, maltodextrin layer, 42, gelatin layer.
Specific implementation mode
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
The every other embodiment that field those of ordinary skill is obtained without making creative work, belongs to this practicality
Novel protected range.
Such as Fig. 1, a kind of bacillus subtilis and trichoderma microcapsule granule, including the double-deck core material, the double-deck wall material, the double-deck core
Material and the double-deck wall material are solid concentric structure, and bacillus subtilis and the sequence of trichoderma microcapsule granule from the inside to surface are the
One core material, 1 trichoderma is also the core of entire bacillus subtilis and trichoderma microcapsule granule, is wrapped in outside the first core material 1
Be that the first core material 1 is filled in 2 saccharomycete biological cell of the first wall material by 2 saccharomycete biological cell parietal layer of the first wall material, packet
Overlay on 2 outer surface of the first wall material is 3 bacillus subtilis fermentation liquor of the second core material, outermost to be made of the double-deck wall material
The second wall material 4 being coated on outside the second core material 3, and 4 internal layer of the second wall material is maltodextrin layer 41, outer layer is gelatin layer 42.
For the grain size of entire bacillus subtilis and trichoderma microcapsule granule within the scope of 0.2-0.5mm, preferred thickness is 0.4mm,
The thickness of first wall material, 2 saccharomycete biological cell parietal layer is in 0.15-0.25 μ ms, preferably 0.21 μm, maltodextrin
For the thickness of layer 41 and gelatin layer 42 respectively in 15-25 μm and 10-20 μ ms, preferred is respectively 20 μm and 12 μm.
While in order to ensure the stability and release performance first of entire bacillus subtilis and trichoderma microcapsule granule
The amount that core material 1 adds is 0.3-0.5 times of 3 additive amount of the second core material, and 0.41 times of when entire bacillus subtilis with it is wooden
Mould agent microcapsule granule structure is stablized the most, and sustained release performance is best.
In order to ensure the accumulation of the viability of bacillus subtilis and Trichoderma and the nutriment of secretion, in the first core material
1 and second be added with bacillus subtilis and Trichoderma in core material 3 respectively culture medium.
In use, bacillus subtilis is mixed with trichoderma microcapsule granule with water, wall material, which is dissolved in water, to be released
3 bacillus subtilis fermentation liquor of the second core material is released, while 1 Trichoderma of the first core material is due to that can secret out of cell wall degradation enzyme
With secondary metabolite and slowly come out by sustained release, the particle of preparation has the double effects of bacillus subtilis and Trichoderma,
And the two release is not interfered.
It is exemplarily described above in conjunction with the utility model of specific embodiment pair, it is clear that the utility model is specifically real
It is now not subject to the restrictions described above, as long as using the various non-realities of the methodology and technical solution progress of the utility model
The improvement of matter, or it is not improved the design of the utility model and technical solution are directly applied into other occasions, this
Within the protection domain of utility model.The scope of protection of the utility model should be with protection domain defined by claims
It is accurate.
Claims (5)
1. a kind of bacillus subtilis and trichoderma microcapsule granule, including the double-deck core material, the double-deck wall material, the double-deck core material and bilayer
Wall material is solid concentric structure, which is characterized in that the double-deck core material and the double-deck wall material from the inside to surface put in order for
First core material (1), the first wall material (2), the second core material (3), the second wall material (4), first core material (1) are trichoderma,
First wall material (2) is saccharomycete biological cell wall, and second core material (3) is bacillus subtilis fermentation liquor, described
The second wall material (4) from the inside to surface include maltodextrin layer (41) and gelatin layer (42).
2. bacillus subtilis according to claim 1 and trichoderma microcapsule granule, which is characterized in that described solid same
Heart diameter of a circle is 0.2-0.5mm.
3. bacillus subtilis according to claim 1 and trichoderma microcapsule granule, which is characterized in that first wall
The thickness of material (2) is 0.15-0.25 μm.
4. bacillus subtilis according to claim 1 and trichoderma microcapsule granule, which is characterized in that the malt paste
The thickness of smart layer (41) is 15-25 μm, and institute gelatin layer (42) thickness is 10-20 μm.
5. bacillus subtilis according to claim 1 and trichoderma microcapsule granule, which is characterized in that first core
Material (1) and the second core material (3) also include culture medium, and the amount of the first core material (1) is 0.3-0.5 times of the second core material (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820128736.0U CN207803320U (en) | 2018-01-25 | 2018-01-25 | A kind of bacillus subtilis and trichoderma microcapsule granule |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820128736.0U CN207803320U (en) | 2018-01-25 | 2018-01-25 | A kind of bacillus subtilis and trichoderma microcapsule granule |
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| CN207803320U true CN207803320U (en) | 2018-09-04 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468056A (en) * | 2019-08-14 | 2019-11-19 | 安徽旭辰生物科技有限公司 | It is a kind of prevent and treat watermelon blight Trichoderma and its application |
| CN111088191A (en) * | 2020-01-08 | 2020-05-01 | 华东理工大学 | Bacillus and trichoderma combined culture method and application |
| CN111838190A (en) * | 2019-04-24 | 2020-10-30 | 山东大学苏州研究院 | Biocontrol microbial inoculum for preventing and treating stem base rot and gummosis as well as preparation method and application thereof |
-
2018
- 2018-01-25 CN CN201820128736.0U patent/CN207803320U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111838190A (en) * | 2019-04-24 | 2020-10-30 | 山东大学苏州研究院 | Biocontrol microbial inoculum for preventing and treating stem base rot and gummosis as well as preparation method and application thereof |
| CN110468056A (en) * | 2019-08-14 | 2019-11-19 | 安徽旭辰生物科技有限公司 | It is a kind of prevent and treat watermelon blight Trichoderma and its application |
| CN111088191A (en) * | 2020-01-08 | 2020-05-01 | 华东理工大学 | Bacillus and trichoderma combined culture method and application |
| CN111088191B (en) * | 2020-01-08 | 2021-04-02 | 华东理工大学 | Bacillus and trichoderma combined culture method and application |
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