CN207792652U - A kind of sterile packing storage device, quickly aseptic subpackaged storage system - Google Patents

Abstract

The utility model provides a kind of sterile packing storage device, including plastic container bottle, sample feeding pipe, the first female Luer component and millipore filter；Sample feeding pipe one end connects the first female Luer component, and the other end is connected to the bore seal of plastic container bottle；Millipore filter is connected to the bore seal of plastic container bottle.The sterile packing memory device structure of the utility model is simple and convenient to operate, without carrying out packing work in superclean bench, without Reusability pipettor, seed bottle is repeatedly added in production strain and seed culture of viruses without will prepare in superclean bench, manpower is saved, efficiency is improved；Overall process realizes packing in sterile pipeline, without uncapping and covering, reduces the contaminated risk of strain and seed culture of viruses；Cryogenic pulverization risk can be not present by strain and virus seed conservation in minus 20 degrees Celsius of environment in the container bottle of plastic material.On this basis, the utility model additionally provides a kind of quickly aseptic subpackaged storage system.

Description

A kind of sterile packing storage device, quickly aseptic subpackaged storage system

Technical field

The utility model is related to biological product technical fields, in particular to a kind of sterile packing storage device, soon The aseptic subpackaged storage system of speed.

Background technology

In the production of biological product technical field, especially bacterial vaccine and viral vaccine, need to prepare largely same batch Secondary or different batches production strains and seed culture of viruses, to meet the production of antigen.

However in traditional production technology, due to the limitation of existing equipment and tool, to bacterial vaccine and viral vaccine Carry out it is aseptic subpackaged, preserve when there is many defects.Especially when utilize 3L~20L bioreactors prepare production strain When with seed culture of viruses, problem becomes apparent protrusion.

When 1, strain and seed culture of viruses being dispensed and preserved in the lab, generally with the cryopreservation tube of 2.0ml or 5.0ml, this The capacity of class cryopreservation tube is too small, it is difficult to and meet antigen inoculation amount when production vaccine, classification is needed to expand numerous strain and seed culture of viruses, so that It takes considerable time.It is therefore desirable to which the strain prepared to be carried out to the packing and preservation of large volume, reduces and expand numerous time.

2, in superclean bench, the production strain and poison of 20ml~1000ml are preserved with centrifuge tube and vial Kind, it is small that there are centrifuge tubes, the excessive problem of packing number.

When 3, being dispensed with vial and preserved strain, seed culture of viruses, need frequently to uncap and cover, polluted risk, And the deficiency that working efficiency is not high, labor intensity is big.Meanwhile vial is very easy to be crushed in -20 DEG C of environment.

4, it in addition, also common in the market frozen bag and preserved strain and seed culture of viruses using disposable sterilized, freezes volume and exists 20ml~100ml freezes small and with high costs.

So how to improve the packing of strain and seed culture of viruses, save set, improves packing and preserve efficiency, reduce pollution risk It is those skilled in the art's urgent problem to be solved with job costs.

Utility model content

The purpose of this utility model is to provide a kind of sterile packing storage device, quickly aseptic subpackaged storage system, To solve tool in the prior art in packing, storage strain and when seed culture of viruses existing inefficiency, flow be cumbersome, pollution risk Greatly, the problems such as use cost is high.

To achieve the goals above, the utility model uses following technical scheme：

A kind of sterile packing storage device provided by the utility model, including plastic container bottle, sample feeding pipe, first Rule Adapter assembly and millipore filter；Described sample feeding pipe one end connects the first female Luer component, the other end and the plastics The bore seal of container bottle is connected to；The millipore filter is connected to the bore seal of the plastic container bottle.

Further, sterile packing storage device further includes sterile tube；The sterile tube is built in the plastic container bottle, Port is connected to the sample feeding pipe thereon, and lower port tilts the bottom for abutting the plastic container bottle.The technology of the technical solution Effect is：Sterile tube upper end connects sample feeding pipe, and lower end abuts the bottom of plastic container bottle.Then in strain and seed culture of viruses recovery, Can the sterile transfer directly from plastic container bottle by strain and seed culture of viruses reduce pollution risk without uncapping.

Further, the sterile tube is medicine food sanitation-grade silicone tube.The technical solution has technical effect that：Food Grade silica gel and medical silica-gel belong to high-quality silica gel, can be used directly in the equipment of contact human skin.The security level Sterile tube have good physiological inertia, not will produce harm to tissue, human body surrounding tissue will not be made to be inflamed. The high- and low-temperature resistance performance of what is more important, medicine food sanitation-grade silicone tube is strong, will not be deformed under high-strength temperature, also not It can decomposing harmful substances.

Further, the plastic container bottle is made of polypropylene material.The technical solution has technical effect that：Polypropylene (Polypropylene is abbreviated as PP) texture material is pure, non-toxic, relative density is small, stable chemical performance, transparency are high, Machine-shaping property is good, further, it is possible in 110~120 celsius temperatures or minus 40 degrees Celsius of low temperature continuous use without Change performance.Plastic containers made of polypropylene material can carry out high-temperature sterilization.Particularly, plastic containers may be configured as it is more Kind different volumes size, such as 100ml, 250ml, 500ml or 1000ml, bottleneck outer diameter are preferably arranged to 24mm.

Further, the female Luer component is made of polypropylene material.The technical solution has technical effect that：Equally , since polypropylene material quality is pure, non-toxic, relative density is small, stable chemical performance, transparency are high, processing forming Can be good, further, it is possible to which continuous use is without changing performance in 110~120 celsius temperatures or minus 40 degrees Celsius of low temperature.It is poly- Female Luer component made of propylene material can also carry out high-temperature sterilization.Particularly, connector outer diameter is preferably arranged to 4~7mm.

Further, the millipore filter is made of polytetrafluoroethylene material.The technical solution has technical effect that：It is poly- The spy that tetrafluoroethene (Poly tetra fluoroethylene, be abbreviated as PTFE) has antiacid alkali resistant, resists various organic solvents Point is practically insoluble in all solvents.Meanwhile polytetrafluoroethylene (PTFE) has the characteristics that heat safe, temperature in use is between -190~250 Between DEG C；Also there is physiological inertia, be chronically implanted as artificial blood vessel and internal organs and have no adverse reaction in vivo.Polytetrafluoroethylene material Manufactured millipore filter can be disinfected with high-temperature sterilization, also not reacted with biological products.

Further, the sample feeding pipe is thermoplastic pipe.The technical solution has technical effect that：Thermoplastic pipe is after the heating Realization sealing can quickly be tightened, ensure the sterile connection of dispensing passage.

Further, sterile packing storage device further includes pipe clamp, and the pipe clamp is clamped in the outer wall of the sample feeding pipe.It should Technical solution has technical effect that：Start pipe clamp after packing, realizes quick sterile packaged.

The utility model also provides a kind of quickly aseptic subpackaged storage system, including bioreactor, discharge nozzle, the second Shandong That adapter assembly, sterile bulb-tubulating machine, meausring apparatus and multiple above-mentioned sterile packing storage devices；The biological respinse Device, the discharge nozzle, the second female Luer component and the sterile bulb-tubulating machine are sequentially connected；The sterile packing storage Device is connect by the first female Luer component with the sterile bulb-tubulating machine；The meausring apparatus is described sterile for measuring Packing storage device weight.

Further, the meausring apparatus is electronic scale.The technical solution has technical effect that：It is accurate that electronic scale measures, It is of low cost using quick.The electronic scale that preferably at most range is 2 kilograms.

The utility model has the beneficial effects that：

1, sterile packing memory device structure is simple and convenient to operate, and need not carry out packing work in superclean bench Make, need not also use pipettor repeatedly, the production strain and seed culture of viruses without will prepare are primary another in superclean bench Secondary addition seed bottle, saves manpower, improves efficiency.

2, sterile packing storage device overall process is dispensed in sterile pipeline, without uncapping and covering, is reduced The contaminated risk of strain and seed culture of viruses.

3, sterile packing storage device uses the container bottle of plastic material, can take the photograph strain and virus seed conservation minus 20 In the environment of family name's degree, the risk of breakage of vial is not present.

4, quickly aseptic subpackaged storage system utilizes multiple sterile packing storage device repetitive operations, reduces production and uses Strain and seed culture of viruses expand numerous time, accurately weigh, quantitative separating and preservation, a people can be completed all operationss, reduce people Work labour costs reduces seed and expands numerous risk brought, improves work efficiency.

Description of the drawings

It, below will be to specific implementation mode in order to illustrate more clearly of the technical solution of specific embodiment of the present invention Attached drawing needed in description is briefly described.It should be evident that the accompanying drawings in the following description is the utility model Some embodiments for those of ordinary skill in the art without creative efforts, can also basis These attached drawings obtain other attached drawings.

Fig. 1 is the structural schematic diagram for the sterile packing storage device that the utility model embodiment provides；

Fig. 2 is the partial enlarged view at I in Fig. 1；

Fig. 3 is the partial enlarged view at K in Fig. 1；

Fig. 4 is the structural schematic diagram for the quick aseptic subpackaged storage system that the utility model embodiment provides.

Reference numeral：

1- plastic container bottles；2- sample feeding pipes；3- the first female Luer components；

4- millipore filters；5- sterile tubes；6- pipe clamps；

7- bioreactors；8- discharge nozzles；9- the second female Luer components；

The sterile bulb-tubulating machines of 10-；11- meausring apparatuses.

Specific implementation mode

The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally The every other embodiment that field those of ordinary skill is obtained without making creative work, belongs to this practicality Novel protected range.

It is in the description of the present invention, it should be noted that term "center", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only The utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specific Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relative importance.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete condition The concrete meaning of language in the present invention.

DESCRIPTION OF THE PRIOR ART：

In the production of biological product technical field, especially bacterial vaccine and viral vaccine, need to prepare largely same batch Secondary or different batches production strains and seed culture of viruses, to meet the production expenditure of antigen.

However in traditional production technology, due to the limitation of existing equipment and tool, to bacterial vaccine and viral vaccine Carry out it is aseptic subpackaged, preserve when there is many defects.Especially when using 3L~20L bioreactors 7 prepare production use bacterium When kind and seed culture of viruses, problem becomes apparent protrusion.

When 1, strain and seed culture of viruses being dispensed and preserved in the lab, generally with the cryopreservation tube of 2.0ml or 5.0ml, this The capacity of class cryopreservation tube is too small, it is difficult to and meet antigen inoculation amount when production vaccine, classification is needed to expand numerous strain and seed culture of viruses, so that It takes considerable time.It is therefore desirable to which the strain prepared to be carried out to the packing and preservation of large volume, reduces and expand numerous time.

2, in superclean bench, the production strain and poison of 20ml~1000ml are preserved with centrifuge tube and vial Kind, it is small that there are centrifuge tubes, the excessive problem of packing number.

When 3, being dispensed with vial and preserved strain, seed culture of viruses, need frequently to uncap and cover, polluted risk, And the deficiency that working efficiency is not high, labor intensity is big.Meanwhile vial is very easy to be crushed in -20 DEG C of environment.

4, it in addition, also common in the market frozen bag and preserved strain and seed culture of viruses using disposable sterilized, freezes volume and exists 20ml~100ml freezes small and with high costs.

The utility model specific embodiment：

Embodiment one：

A kind of sterile packing storage device is present embodiments provided, wherein：Fig. 1 is what the utility model embodiment provided The structural schematic diagram of sterile packing storage device；Fig. 2 is the partial enlarged view at I in Fig. 1；Fig. 3 is the part at K in Fig. 1 Enlarged drawing.As shown in Figure 1, sterile packing storage device includes plastic container bottle 1, sample feeding pipe 2, the first female Luer component 3 With millipore filter 4.Specifically, 2 one end of sample feeding pipe connects the first female Luer component 3, and the other end is interior with plastic container bottle 1 Chamber sealing connection.And millipore filter 4 is connected to the bore seal of plastic container bottle 1.

Sterile packing storage device provided by the utility model can preferably solve tool in the prior art and divide Dress, storage strain and when seed culture of viruses existing inefficiency, the problems such as flow is cumbersome, pollution risk is big, use cost is high：First, Sterile packing memory device structure is simple and convenient to operate, and need not be carried out packing work in superclean bench, also not needed Pipettor is used repeatedly, it is not necessary that seed is added with strain and seed culture of viruses in the production prepared again and again in superclean bench Bottle, saves manpower, improves efficiency.Secondly, sterile packing storage device overall process is divided in 5 tunnel of sterile tube Dress, without uncapping and covering, reduces the contaminated risk of strain and seed culture of viruses.Again, sterile packing storage device is using modeling Expect the container bottle of material, the broken wind of vial can be not present by strain and virus seed conservation in minus 20 degrees Celsius of environment Danger.

On the basis of the above embodiments, as shown in Figure 1,3, further, sterile packing storage device further includes nothing Tube 5.Wherein, sterile tube 5 is built in plastic container bottle 1, and port is connected to sample feeding pipe 2 thereon, and lower port, which tilts, abuts plastics The bottom of container bottle 1.The sterile packing storage device of the structure, since 5 upper end of sterile tube connects sample feeding pipe 2, lower end abuts It the bottom of plastic container bottle 1 can be by strain and seed culture of viruses sterile turn directly from plastic container bottle 1 in strain and seed culture of viruses recovery It moves, without uncapping, reduces pollution risk.

On the basis of the above embodiments, further, sterile tube 5 preferably uses medicine food sanitation-grade silicone tube.It should In the sterile packing storage device of structure, food grade silicone and medical silica-gel belong to high-quality silica gel, can be used directly for In the equipment for contacting human skin.The sterile tube 5 of the security level has good physiological inertia, not will produce to tissue Harm, will not make human body surrounding tissue be inflamed.What is more important, the high- and low-temperature resistance of medicine food sanitation-grade silicone tube Performance is strong, will not be deformed under high-strength temperature, also will not decomposing harmful substances.

On the basis of the above embodiments, further, plastic container bottle 1 is preferably made of polypropylene material.The knot In the sterile packing storage device of structure, since polypropylene (Polypropylene is abbreviated as PP) texture material is pure, nontoxic Property, relative density is small, stable chemical performance, transparency are high, machine-shaping property is good, further, it is possible at 110~120 degrees Celsius Continuous use is without changing performance in high temperature or minus 40 degrees Celsius of low temperature.So plastic containers made of polypropylene material can To carry out high-temperature sterilization.Particularly, plastic containers may be configured as a variety of different volumes sizes, such as 100ml, 250ml, 500ml Or 1000ml etc., bottleneck outer diameter are preferably arranged to 24mm.

On the basis of the above embodiments, further, female Luer component is preferably made of polypropylene material.The knot In the sterile packing storage device of structure, likewise, since polypropylene material quality is pure, non-toxic, relative density is small, chemical Performance is stable, transparency is high, machine-shaping property is good, further, it is possible in 110~120 celsius temperatures or 40 degrees Celsius minus Continuous use is without changing performance in low temperature.So female Luer component made of polypropylene material can also carry out high-temperature sterilization. Particularly, connector outer diameter is preferably arranged to 4~7mm.

On the basis of the above embodiments, further, millipore filter 4 is preferably made of polytetrafluoroethylene material. In the sterile packing storage device of the structure, since (Poly tetra fluoroethylene, are abbreviated as polytetrafluoroethylene (PTFE) PTFE) have the characteristics that antiacid alkali resistant, resist various organic solvents, be practically insoluble in all solvents.Meanwhile polytetrafluoroethylene (PTFE) has There is heat safe feature, temperature in use is between minus between 190~250 degrees Celsius；Also there is physiological inertia, as artificial blood vessel and Internal organs are chronically implanted to have no adverse reaction in vivo.So millipore filter 4 made of polytetrafluoroethylene material can be disappeared with high-temperature sterilization Poison processing, does not also react with biological products.

On the basis of the above embodiments, further, sample feeding pipe 2 is thermoplastic pipe.Thermoplastic pipe after the heating can Quickly tighten and realize sealing, ensures the sterile connection of dispensing passage.

On the basis of the above embodiments, as shown in Figure 1, 2, further, sterile packing storage device further includes pipe Folder 6, which is clamped in the outer wall of sample feeding pipe 2.Start pipe clamp 6 after packing, can realize quick sterile packaged.

The utility model also provides a kind of quickly aseptic subpackaged storage system, wherein：Fig. 4 carries for the utility model embodiment The structural schematic diagram of the quick aseptic subpackaged storage system supplied.As shown in figure 4, quickly aseptic subpackaged storage system includes that biology is anti- Answer device 7, discharge nozzle 8, the second female Luer component 9, sterile bulb-tubulating machine 10, meausring apparatus 11 and multiple above-mentioned sterile points Fill storage device.Wherein, bioreactor 7, discharge nozzle 8, the second female Luer component 9 are sequentially connected with sterile bulb-tubulating machine 10. Sterile packing storage device is connect by the first female Luer component 3 with sterile bulb-tubulating machine 10.And meausring apparatus 11 is for surveying Measure the weight of sterile packing storage device.

On the basis of the above embodiments, as shown in figure 4, further, meausring apparatus 11 preferably uses electronic scale.Electronics It is accurate that scale measures, of low cost using quick.The electronic scale that preferably at most range is 2 kilograms.

The concrete operation step of quick aseptic subpackaged storage system is as follows：

1, first by plastic container bottle 1, sample feeding pipe 2, the first female Luer component 3, millipore filter 4, sterile tube 5, pipe clamp 6 It is installed etc. each component, the holding bottle component as strain and seed culture of viruses.

2, holding bottle component is passed through into high temperature (121 degrees Celsius) high pressure sterilization 30 minutes.

3, holding bottle component is passed through into 10 general of sterile bulb-tubulating machine, the second female Luer component 9, discharge nozzle 8 and biological respinse 7 sterile welding of device forms pipeline.

4, holding bottle component is placed on meausring apparatus 11, removes tare weight.

5, it pressurizes to bioreactor 7, production strain and seed culture of viruses is aseptic subpackaged in plastic container bottle 1；Pass through title After amount device 11 obtains scheduled component, pipe clamp 6 is clamped, while allowing 7 pressure release of bioreactor.

6, by sterile bulb-tubulating machine 10 by another empty 7 sterile welding of holding bottle component and bioreactor, simultaneously First female Luer component 3 sterile can be welded on the holding bottle that just packing has been completed.

7, above step is repeated, until all production strains, packing finishes.

8, all holding bottle components minus 20 DEG C of environment are put into preserve.

Further, quickly aseptic subpackaged storage system can also make further operating：It will be in the holding bottle component that kept Strain and seed culture of viruses, after directly thawing access bioreactor 7 in.

Concrete operation step is as follows：

1, it by the strain and seed culture of viruses in holding bottle component, directly thaws.

2, by sterile bulb-tubulating machine 10 by discharge nozzle 8 and 2 sterile welding of sample feeding pipe, by strain and seed culture of viruses aseptic inoculation to life It is cultivated in object reactor 7.To realize the production of strain and seed culture of viruses to antigen completely without the transmission of tube 5 and closed culture.

Above-mentioned two operating process can one man operation, reduce the generation time of strain and seed culture of viruses, reduce cost of labor And seed expands numerous risk brought, and improves work efficiency.

Finally it should be noted that：The above various embodiments is only to illustrate the technical solution of the utility model, rather than limits it System；Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should Understand：It still can be with technical scheme described in the above embodiments is modified, either to which part or whole Technical characteristic carries out equivalent replacement；And these modifications or replacements, this practicality that it does not separate the essence of the corresponding technical solution are new The range of each embodiment technical solution of type.

Claims (10)

1. a kind of sterile packing storage device, which is characterized in that including plastic container bottle, sample feeding pipe, the first female Luer group Part and millipore filter；

Described sample feeding pipe one end connects the first female Luer component, and the bore seal of the other end and the plastic container bottle connects It is logical；

The millipore filter is connected to the bore seal of the plastic container bottle.

2. sterile packing storage device according to claim 1, which is characterized in that further include sterile tube；It is described sterile Pipe is built in the plastic container bottle, and port is connected to the sample feeding pipe thereon, and lower port, which tilts, abuts the plastic container bottle Bottom.

3. sterile packing storage device according to claim 2, which is characterized in that the sterile tube is defended for medicine food Raw grade silicone tube.

4. according to the sterile packing storage device of claims 1 to 3 any one of them, which is characterized in that the plastic containers Bottle is made of polypropylene material.

5. according to the sterile packing storage device of claims 1 to 3 any one of them, which is characterized in that the female Luer Component is made of polypropylene material.

6. according to the sterile packing storage device of claims 1 to 3 any one of them, which is characterized in that the micro porous filtration Device is made of polytetrafluoroethylene material.

7. according to the sterile packing storage device of claims 1 to 3 any one of them, which is characterized in that the sample feeding pipe is Thermoplastic pipe.

8. sterile packing storage device according to claim 7, which is characterized in that further include pipe clamp, the pipe clamp folder Hold the outer wall in the sample feeding pipe.

9. a kind of quickly aseptic subpackaged storage system, which is characterized in that including bioreactor, discharge nozzle, the second female Luer Component, sterile bulb-tubulating machine, meausring apparatus and multiple such as sterile packing storage devices of claim 1~8 any one of them；

The bioreactor, the discharge nozzle, the second female Luer component and the sterile bulb-tubulating machine are sequentially connected；Institute The weight for stating sterile packing storage device is connect by the first female Luer component with the sterile bulb-tubulating machine；

The meausring apparatus is used to measure the weight of the sterile packing storage device.

10. quickly aseptic subpackaged storage system according to claim 9, which is characterized in that the meausring apparatus is electronics Scale.