CN207703777U - A kind of capillary electrophoresis - Google Patents
A kind of capillary electrophoresis Download PDFInfo
- Publication number
- CN207703777U CN207703777U CN201721918811.5U CN201721918811U CN207703777U CN 207703777 U CN207703777 U CN 207703777U CN 201721918811 U CN201721918811 U CN 201721918811U CN 207703777 U CN207703777 U CN 207703777U
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- Prior art keywords
- valve rod
- storage tank
- mounting plate
- magnet mounting
- capillary
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Abstract
The utility model is related to a kind of capillary electrophoresis,Including capillary body and syringe,Syringe includes syringe body,Valve rod storage tank is offered on its upper surface,Convex block storage tank is opened up on the bottom surface of valve rod storage tank,Fluid outlet channels are equipped in syringe body,The bottom end of fluid outlet channels and the connection of capillary body,It is provided with valve rod in valve rod storage tank,The bottom surface of valve rod is equipped with convex block,The top of syringe body is provided with connecting flange,Its surface is provided with magnet,Its top is connect with magnet mounting plate,The first electromagnet is provided on the bottom surface of magnet mounting plate,Magnet mounting plate is equipped with slide bar,The bottom end of slide bar is connect with limited block,Elastic device is additionally provided between limited block and connecting flange,It is further opened with working chamber on the top surface of valve rod,Piston is provided in working chamber,It is provided with gas inlet pipe on the top surface of magnet mounting plate,Drive cylinder and solenoid valve.The capillary electrophoresis of the utility model can effectively control electroosmotic flow.
Description
Technical field
The utility model is related to a kind of electrophoretic apparatus more particularly to a kind of capillary electrophoresis.
Background technology
Capillary Electrophoresis (capillary electrophoresis, CE), be one kind using capillary as split tunnel, with
High-voltage dc is the novel liquid-phase isolation technics of driving force.It enables analytical chemistry from microlitre horizontally entering into a nanoliter water
It is flat, and make it possible single cell analysis or even single molecule analysis.Point of our large biological molecule of long-standing problem such as protein
Also therefore there is new favourable turn from analysis.The basic structure of capillary electrophoresis system includes sampling system, buffering liquid groove, high-voltage electricity
Source, detector, control system and data processing system.When work, the both ends of capillary submerge respectively in two buffering liquid grooves,
Buffering liquid groove is connect with the positive and negative anodes of high voltage power supply respectively.For capillary in electrophoresis, the solvent in capillary is because of axial DC electricity
Field action and directed flow i.e. electroosmotic flow occurs.Electric osmose is caused by localization charge.Localization charge refers to being firmly bonded to tube wall
Ion or charged group upper, cannot migrate.Counter ion in localization charge attraction solution and the electric double layer constituted with it,
Cause solvent global orientation under electric field action (and collision effect) mobile and form electroosmotic flow, electric osmose is played the part of in electrophoretic separation
Key player is drilled, is a kind of electrokinetic phenomena generated with electrophoresis.In most cases, electroosmotic flow speed is the 5- of electrophoretic velocity
7 times.Existing capillary device can not efficiently control the size of electroosmotic flow, to influence to be separated by electrophoresis.
In view of the above shortcomings, the designer, is actively subject to research and innovation, to found a kind of new structural capillary
Electrophoresis tube device makes it with more the utility value in industry.
Utility model content
In order to solve the above technical problems, the purpose of this utility model is to provide a kind of capillary that can effectively control electroosmotic flow
Electrophoresis tube device.The capillary electrophoresis of the utility model can be controlled by controlling absorption with the localization charge on tube wall
The size of electroosmotic flow.
The capillary electrophoresis of the utility model, including capillary body and syringe, the syringe include injection
Device ontology offers valve rod storage tank on the upper surface of the syringe body, is opened up on the bottom surface of the valve rod storage tank convex
Block storage tank, the syringe body is interior to be equipped with the liquid output that syringe body bottom surface is extended to from convex block storage tank medial surface
The top in channel, the fluid outlet channels is connected to convex block storage tank, and the bottom end of fluid outlet channels passes through pipeline and capillary
The inner cavity of tube body is connected to, and valve rod compatible with valve rod storage tank, the bottom of the valve rod are provided in the valve rod storage tank
Face is equipped with the convex block being adapted to convex block storage tank, and the top of the syringe body is provided with connecting flange, the connection method
Blue surface is provided with magnet, and the top of the valve rod passes through connecting flange and connect with magnet mounting plate, and the top of valve rod is solid
Surely it is set on the magnet mounting plate, the first electromagnetism being connected with magnet mounting plate is provided on the bottom surface of magnet mounting plate
Iron is additionally provided with slide bar on magnet mounting plate, and the top of the slide bar is fixedly installed on the lower surface of magnet mounting plate, slide bar
Bottom end pass through and connecting flange and connect with limited block, elastic device, institute are additionally provided between the limited block and connecting flange
The top for stating elastic device is contacted with connecting flange, and bottom end is contacted with limited block, and work is further opened on the top surface of the valve rod
Make chamber, piston is provided in the working chamber, the piston is contacted with the medial surface of working chamber, the top surface of the magnet mounting plate
On be provided with the gas inlet pipe being connected to working chamber, driving cylinder and solenoid valve is additionally provided on the top surface of magnet mounting plate,
The output end of the driving cylinder is connected to gas inlet pipe, and the input terminal of cylinder is driven to connect by solenoid valve and external air source
It connects, the stem internal is provided with the liquid input channel that convex block bottom surface is extended to from working chamber bottom surface.
Further, the capillary electrophoresis of the utility model is additionally provided with and the work on the lateral surface of valve rod
The sample input pipe that chamber, liquid input channel are connected to.
Further, the capillary electrophoresis of the utility model is provided with the second electricity on the top surface of the connecting flange
Magnet.
Further, the capillary electrophoresis of the utility model, the capillary body is interior to be equipped with pillar, the pillar
It is inside provided with cathode tube, anode tube is provided on the capillary body lateral surface, the cathode tube and anode tube are respectively by gold
Belong to material to be made, and one end of cathode tube and external power supply connects, the other end of anode tube and external power supply connects.
According to the above aspect of the present invention, the utility model has at least the following advantages：The capillary electrophoresis of the utility model, note
The setting of emitter so that operating personnel can be injected by syringe into capillary at any time with a certain concentration, pH value, viscosity
Buffer solution or surfactant, and then influence electroosmotic flow size and electrophoretic separation process.When specific works, outside control
System connects the connection between driving cylinder and external air source by solenoid valve, and external air source is transmitted to work by gas inlet pipe
Make intracavitary, and the piston in working chamber is acted, piston moves down under the pressure of the gas, and makes below piston
Liquid is transmitted in liquid input channel, and at the same time, the first electromagnet is opened, and the magnet being oppositely arranged with the first electromagnet exists
Overcome the elastic force of elastic device to act under the repulsive interaction of first electromagnet and syringe body is driven to move down, to open
Connection between liquid input channel and fluid outlet channels, be located at liquid input channel in sample by convex block storage tank into
Enter into fluid outlet channels, finally exported from fluid outlet channels into capillary lumen, syringe passes through piston, valve rod etc.
The setting of structure can control automatically entering for liquid, meanwhile, operating personnel personnel can be by controlling the first electromagnet
Magnetic force size control the distance that syringe body moves down, it is real to the size of indirect control fluid outlet channels switching value
The micro input of existing liquid, it is simple in structure, it is easy to operate.
In conclusion the capillary electrophoresis of the utility model can effectively control electroosmotic flow, can be inhaled by controlling
The localization charge on tube wall is invested to control the size of electroosmotic flow.
The above description is merely an outline of the technical solution of the present invention, in order to better understand the skill of the utility model
Art means, and can be implemented in accordance with the contents of the specification, below on the preferred embodiment of the present invention and the accompanying drawings in detail
It describes in detail bright as after.
Description of the drawings
Fig. 1 is the structural schematic diagram of the capillary electrophoresis of the utility model；
Fig. 2 is the structural schematic diagram of syringe；
Fig. 3 is the structural schematic diagram of syringe body.
In figure, 1：Capillary body；2：Pillar；3：Cathode tube；4：Anode tube；5：Electromagnetic coil；6：Syringe；7：Injection
Device ontology；8：Valve rod storage tank；9：Convex block storage tank；10：Fluid outlet channels；11：Valve rod；12：Convex block；13：Connecting flange；
14：Magnet；15：Magnet mounting plate；16：First electromagnet；17：Slide bar；18：Limited block；19：Elastic device；20：Working chamber；
21：Piston；22：Gas inlet pipe；23：Drive cylinder；24：Solenoid valve；25：Liquid input channel；26：Sample input pipe；27：
Second electromagnet.
Specific implementation mode
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is described in further detail.Below
Embodiment is not intended to limit the scope of the present invention for illustrating the utility model.
Referring to Fig. 1 to Fig. 3, the controllable electrophoresis capillary device of absorption of one preferred embodiment of the utility model, including capillary
Tube body 1, the cathode tube 3 being set in pillar, is set on the outside of capillary body the pillar 2 being set in capillary body
Anode tube 4 on face, cathode tube and anode tube are made of metal material respectively, and one end of cathode tube and external power supply connects
It connects, the other end connection of anode tube and external power supply.
The setting of anode tube and cathode tube so that radial electric field can be generated in capillary body, as long as operating personnel are logical
The mode for crossing control external power supply size can control size and the direction of the radial electric field, and tube wall is adsorbed in reach control
On localization charge size purpose, further influence electroosmotic flow size and electrophoretic separation process.
Preferably, the lateral surface of anode tube is equipped with the installing pipe being made of plastics, it is equipped in installing pipe and surrounds anode
The electromagnetic coil 5 of pipe setting.
The setting of electromagnetic coil so that electromagnetic coil can be connect by operating personnel with external high frequency power supply, to make electricity
Magnetic coil generates the electromagnetic field of alternation, is set to the anode tube made of magnetic conductive metal material in electromagnetic field in electromagnetic induction
Certain heat can be generated under the action of effect, to be heated to capillary, and then influence electroosmotic flow size and electrophoresis
The process of separation.
Preferably, it further includes syringe 6 to adsorb controllable electrophoresis capillary device, syringe includes syringe body 7, note
Valve rod storage tank 8 is offered on the upper surface of emitter ontology, and convex block storage tank 9, syringe are opened up on the bottom surface of valve rod storage tank
The fluid outlet channels 10 that syringe body bottom surface is extended to from convex block storage tank medial surface, fluid outlet channels are equipped in ontology
Top be connected to convex block storage tank, the bottom end of fluid outlet channels is connected to by pipeline with the inner cavity of capillary body, valve rod
Valve rod 11 compatible with valve rod storage tank is provided in storage tank, the bottom surface of valve rod is convex equipped with being adapted to convex block storage tank
The top of block 12, syringe body is provided with connecting flange 13, and the surface of connecting flange is provided with magnet 14, and the top of valve rod is worn
It crosses connecting flange and is connect with magnet mounting plate 15, the top of valve rod is fixedly installed on magnet mounting plate, magnet mounting plate
It is provided with the first electromagnet 16 being connected with magnet mounting plate on bottom surface, slide bar 17 is additionally provided on magnet mounting plate, slide bar
Top is fixedly installed on the lower surface of magnet mounting plate, and the bottom end of slide bar passes through connecting flange and connect with limited block 18, limits
It is additionally provided with elastic device 19 between position block and connecting flange, the top of elastic device is contacted with connecting flange, bottom end and limit
Position block contacts, and is further opened with working chamber 20 on the top surface of valve rod, piston 21, the inside of piston and working chamber are provided in working chamber
Face contacts, and is provided with the gas inlet pipe 22 being connected to working chamber on the top surface of magnet mounting plate, on the top surface of magnet mounting plate
It is additionally provided with driving cylinder 23 and solenoid valve 24, drives the output end of cylinder to be connected to gas inlet pipe, drives the input of cylinder
End is connect by solenoid valve with external air source, and stem internal is provided with the liquid input that convex block bottom surface is extended to from working chamber bottom surface
Channel 25.
The setting of syringe so that operating personnel can think that injection has certain dense in capillary by syringe at any time
The buffer solution or surfactant of degree, pH value, viscosity, and then influence the size of electroosmotic flow and the process of electrophoretic separation.Specific work
When making, external control system connects the connection between driving cylinder and external air source by solenoid valve, and external air source passes through gas
Input pipe is transmitted in working chamber, and is acted to the piston in working chamber, and piston moves down under the pressure of the gas, and
So that the liquid below piston is transmitted in liquid input channel, at the same time, the first electromagnet is opened, with the first electromagnetism iron phase
Overcome the elastic force of elastic device to act under the repulsive interaction of the first electromagnet the magnet of setting and drive syringe body to
Lower movement, to open the connection between liquid input channel and fluid outlet channels, the sample in liquid input channel
It is entered in fluid outlet channels by convex block storage tank, is finally exported from fluid outlet channels into capillary lumen, injected
Device can control automatically entering for liquid by piston, the isostructural setting of valve rod, meanwhile, operating personnel personnel can
The distance that syringe body moves down is controlled by controlling the magnetic force size of the first electromagnet, to which the output of indirect control liquid is logical
The size of road switching value realizes the micro input of liquid, simple in structure, easy to operate.
Preferably, the sample input pipe 26 being connected to working chamber, liquid input channel is additionally provided on the lateral surface of valve rod.
The setting of sample input pipe facilitates operating personnel by buffer solution or table with a certain concentration, pH value, viscosity
Face activating agent is stored in working chamber.
Preferably, the second electromagnet 27 is provided on the top surface of connecting flange.
The setting of second electromagnet further enhances repulsive force of first electromagnet to connecting flange so that connection method
Blue and syringe body can move down under the repulsion force effect of the first electromagnet and the second electromagnet.
Preferably, elastic device is spring.
Preferably, the cathode of cathode tube and external power supply connects, the anode of anode tube and external power supply connects.
Above are merely preferred embodiments of the utility model, it is not intended to limit the utility model, it is noted that for
For those skilled in the art, without deviating from the technical principle of the utility model, if can also make
Dry improvement and modification, these improvements and modifications also should be regarded as the scope of protection of the utility model.
Claims (4)
1. a kind of capillary electrophoresis, it is characterised in that：Including capillary body and syringe, the syringe includes injection
Device ontology offers valve rod storage tank on the upper surface of the syringe body, is opened up on the bottom surface of the valve rod storage tank convex
Block storage tank, the syringe body is interior to be equipped with the liquid output that syringe body bottom surface is extended to from convex block storage tank medial surface
The top in channel, the fluid outlet channels is connected to convex block storage tank, and the bottom end of fluid outlet channels passes through pipeline and capillary
The inner cavity of tube body is connected to, and valve rod compatible with valve rod storage tank, the bottom of the valve rod are provided in the valve rod storage tank
Face is equipped with the convex block being adapted to convex block storage tank, and the top of the syringe body is provided with connecting flange, the connection method
Blue surface is provided with magnet, and the top of the valve rod passes through connecting flange and connect with magnet mounting plate, and the top of valve rod is solid
Surely it is set on the magnet mounting plate, the first electromagnetism being connected with magnet mounting plate is provided on the bottom surface of magnet mounting plate
Iron is additionally provided with slide bar on magnet mounting plate, and the top of the slide bar is fixedly installed on the lower surface of magnet mounting plate, slide bar
Bottom end pass through and connecting flange and connect with limited block, elastic device, institute are additionally provided between the limited block and connecting flange
The top for stating elastic device is contacted with connecting flange, and bottom end is contacted with limited block, and work is further opened on the top surface of the valve rod
Make chamber, piston is provided in the working chamber, the piston is contacted with the medial surface of working chamber, the top surface of the magnet mounting plate
On be provided with the gas inlet pipe being connected to working chamber, driving cylinder and solenoid valve is additionally provided on the top surface of magnet mounting plate,
The output end of the driving cylinder is connected to gas inlet pipe, and the input terminal of cylinder is driven to connect by solenoid valve and external air source
It connects, the stem internal is provided with the liquid input channel that convex block bottom surface is extended to from working chamber bottom surface.
2. capillary electrophoresis according to claim 1, it is characterised in that：It is additionally provided on the lateral surface of valve rod and institute
State working chamber, the sample input pipe that liquid input channel is connected to.
3. capillary electrophoresis according to claim 1, it is characterised in that：It is provided on the top surface of the connecting flange
Second electromagnet.
4. capillary electrophoresis according to claim 1, it is characterised in that：Be equipped in the capillary body pillar,
It is provided with cathode tube in the pillar, anode tube, the cathode tube and anode tube are provided on the capillary body lateral surface
It is made of metal material respectively, and one end of cathode tube and external power supply connects, the other end of anode tube and external power supply connects
It connects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721918811.5U CN207703777U (en) | 2017-12-29 | 2017-12-29 | A kind of capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721918811.5U CN207703777U (en) | 2017-12-29 | 2017-12-29 | A kind of capillary electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207703777U true CN207703777U (en) | 2018-08-07 |
Family
ID=63030666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721918811.5U Expired - Fee Related CN207703777U (en) | 2017-12-29 | 2017-12-29 | A kind of capillary electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207703777U (en) |
-
2017
- 2017-12-29 CN CN201721918811.5U patent/CN207703777U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180807 Termination date: 20181229 |