CN207685302U - A kind of elution pipe and washing device - Google Patents
A kind of elution pipe and washing device Download PDFInfo
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- CN207685302U CN207685302U CN201721749464.8U CN201721749464U CN207685302U CN 207685302 U CN207685302 U CN 207685302U CN 201721749464 U CN201721749464 U CN 201721749464U CN 207685302 U CN207685302 U CN 207685302U
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- elution
- pipe
- cavity
- stirring
- room
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Abstract
The utility model is related to a kind of elution pipe and washing devices, belong to biological extraction element.Pipes of the elution Guan Weiyi with bottom opening, it is characterised in that：It is outwardly projecting with the cavity being connected inside pipe to be equipped with one for bottom interposition in the pipe.The utility model becomes upper and lower two chambers by pipe is eluted, epicoele is the vertical component of pipe, it is easy to bar magnet and stirring set is held into, cavity of resorption is the areola that epicoele evagination is formed, and is used to form elution room, in elution process, elution outdoor face is combined closely with extraction apparatus fire-bar, ensures that heating is more abundant, stirring, which covers bottom plane, will elute that room is suitable for reading to cover closing in vertical structure bottom, independent elution room is formed, the evaporation of eluent in temperature-rise period is effectively avoided；Meanwhile being moved up and down by bar magnet, indoor magnetic bead is eluted under the action of magnetic field force and gravity, it is continuous to move, efficiently elution is realized to the large biological molecule of magnetic bead surfaces, and eluent will not the spluttering extremely upper intracavitary of elution pipe.
Description
Technical field
The utility model is related to a kind of biological extraction equipments, a kind of elution pipe more particularly to extraction large biological molecule and
Washing device.
Background technology
Large biological molecule, including DNA, RNA and protein are extracted using paramagnetic particle method, operating procedure is simple, is easy to automatic
Change, is more and more applied in automation extraction element.It extracts principle, mainly uses nanotechnology to superparamagnetic
The surface of property nano particle is improved, and for different large biological molecules, takes different surface modifications.The magnetic being modified
Pearl, can be on micro interface, the large biological molecules such as special identification nucleic acid, albumen, and efficiently combines therewith.Guanidine hydrochloride,
Under the action of the buffer environments such as guanidinium isothiocyanate and externally-applied magnetic field, from blood, animal tissue, food, pathogenic microorganism equal samples
In isolate DNA and RNA, be mainly used in clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food
The multiple fields such as product safety detection, molecular biology research.
The key step of the large biological molecule of paramagnetic particle method extraction at present is divided into cracking, combination, washing and elution, and big multipotency exists
Extraction process is completed in 30-40 minutes, it is easy to operate, compared with conventional method, it is more easy to realize automation and high throughput, has 96 at present
The processing to 96 samples can be realized with the extraction time of a sample in the nucleic acid automatic extracting instrument in hole, the realization of automation,
Error caused by avoiding human factor, it is also safer.Meanwhile the specific binding of magnetic bead and large biological molecule to extract
Product purity higher, the above are all that conventional method is too far behind to catch up, and more and more producers put into automation magnetic bead and carry
In the research and development of taking equipment.
The factor of influence paramagnetic particle method extraction efficiency, mainly lysis efficiency, magnetic bead joint efficiency and elution efficiency etc., wherein
Elution efficiency, in addition to related to elution buffer condition and magnetic bead itself, the study find that, the design also close phase with elution hole
It closes.Currently, the consumptive material of cooperation paramagnetic particle method extraction automation equipment, is deep-well plates mostly, has single reagent and 96 orifice plates etc. different
Type, according to actual demand, typically sample pore volume is larger, remaining each hole specification is identical (Fig. 2), and in practical applications,
To ensure eluted product concentration, elutes effluent volume in pipe 1 and be less than 50 μ L, in elution process, pass through the band stirring set of bar magnet 3
2 stirring repeatedly causes the eluent 4 containing magnetic bead to stir, to promote sample elution, but in agitation, it is easy to cause
Eluent splashes on elution 1 inner wall of pipe, causes to elute insufficient；It, can be right by extraction apparatus fire-bar 5 meanwhile in elution process
Eluent in deep-well plates is heated, and to improve elution efficiency, but since elution liquid measure is small, heating process, which easily causes, washes
De- liquid evaporation, and then keep elution process more difficult.
Invention content
For the defects of above-mentioned field, the utility model provides a kind of elution pipe, which is two-chamber connectivity structure,
Epicoele vertical structure is easy to bar magnet and stirring set movement；Lower intracavitary places eluent and magnetic bead, forms individually elution room, washes
Stirring set bottom cover avoids the evaporation in temperature-rise period, and the up and down motion of bar magnet on elution room mouthful during de-,
It will not cause eluent spluttering.
A kind of elution pipe, pipes of the elution Guan Weiyi with bottom opening, it is characterised in that：Bottom interposition in the pipe
It is outwardly projecting with the cavity being connected inside pipe to be equipped with one.
The cavity bottom and pipe bottom are arc surface, and bottom and pipe bottom junctions are arc transition in cavity.
The cavity is U-shaped or bowl-shape.
A kind of washing device, including above-mentioned elution pipe and magnetic stirring apparatus, the magnetic stirring apparatus is stirred including a tubulose
Set and the bar magnet in stirring set are mixed, the tubulose stirring set can be held into the pipe of elution pipe, and stir the bottom energy of set
It is covered in the opening of cavity.
The utility model elutes pipe structure by change, and elution pipe is become upper and lower two chambers.Epicoele is the vertical of pipe
Part is easy to bar magnet and stirs to cover be held into, and cavity of resorption is the cavity that epicoele evagination is formed, and is formed and elutes room, in elution process, is eluted
Outdoor face is combined closely with extraction apparatus fire-bar, ensures that heating is more abundant, and stirring set bottom plane is in vertical structure bottom
It will elute that room is suitable for reading to cover closing, effectively avoid the evaporation of eluent in temperature-rise period；Meanwhile being moved up and down by bar magnet, it washes
Indoor magnetic bead is taken off under the action of magnetic field force and gravity, is constantly moved, and the large biological molecule of magnetic bead surfaces is realized and is efficiently washed
It is de-, and eluent will not the spluttering extremely upper intracavitary of elution pipe.
Description of the drawings
Fig. 1 deep-well plates elute hole schematic diagram (prior art),
Fig. 2 the utility model elution pipe,
Fig. 3 the utility model washing devices,
Each label lists as follows in figure：
1- elution pipes, 2- stirring sets, 3- bar magnets, eluents of the 4- containing magnetic bead, 5- extracting solution fire-bars, 11- circles
Pipe, 12- cavitys, 21- stirring sets bottom.
Specific implementation mode
The utility model is described in further detail below in conjunction with the accompanying drawings.
As shown in Fig. 2, the elution pipes 11 of the Guan Weiyi with bottom opening of the utility model, in the pipe 11 among bottom
Position has one outwardly projecting with the cavity 12 being connected inside pipe 11.Existing elution pipe is changed to communicate above and below two
A chamber, epicoele are pipe 11, are easy to bar magnet and stirring set is held into, cavity of resorption is the cavity 12 that epicoele evagination is formed, and forms elution room.
It is preferred that：12 bottom of the cavity and 11 bottom of pipe are arc surface, and bottom is connect with 11 bottom of pipe in cavity 12
Place is arc transition.More preferably：The cavity 12 is U-shaped or bowl-shape.The bottom of arc surface is conducive to pouring out for eluted product.
As shown in figure 3, being the washing device of the elution pipe comprising the utility model, the magnetic stirring apparatus includes a pipe
Shape stirring set 2 and the bar magnet 3 in stirring set 2, magnetic stirring apparatus drive stirring set 2 and bar magnet 3 to stretch into and wash by mechanical arm
In the pipe 11 of de- pipe, and stirring set bottom 21 can be covered in the opening of cavity 12, and being added in cavity 12 has the elution containing magnetic bead
Liquid 4.In extraction process, extracting solution fire-bar 5 is wrapped in outside cavity 12, and magnetic stirring apparatus is powered, and bar magnet 3 moves up and down, in magnetic force
Under effect, the magnetic bead also agitation up and down in eluent.Since stirring set 21 plane of bottom covers closing by room opening is eluted, formed
Independent elution room, effectively avoids the evaporation of eluent in temperature-rise period；While and eluent will not spluttering extremely elution pipe epicoele
It is interior, efficiently elution is realized to the large biological molecule of magnetic bead surfaces.
Claims (4)
1. a kind of elution pipe, pipes of the elution Guan Weiyi with bottom opening, it is characterised in that：Bottom centre position in the pipe
Have one outwardly projecting with the cavity being connected inside pipe.
2. elution pipe according to claim 1, it is characterised in that：The cavity bottom and pipe bottom are arc surface, empty
Intracavitary bottom is arc transition with pipe bottom junctions.
3. elution pipe according to claim 2, it is characterised in that：The cavity is U-shaped or bowl-shape.
4. a kind of washing device, it is characterised in that：Including claim 1-3 any the elution pipe and magnetic stirring apparatus, institute
It includes a tubulose stirring set and the bar magnet in stirring set to state magnetic stirring apparatus, and the tubulose stirring set can be held into elution pipe
Pipe in, and the bottom for stirring set can be covered in the opening of cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721749464.8U CN207685302U (en) | 2017-12-15 | 2017-12-15 | A kind of elution pipe and washing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721749464.8U CN207685302U (en) | 2017-12-15 | 2017-12-15 | A kind of elution pipe and washing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207685302U true CN207685302U (en) | 2018-08-03 |
Family
ID=62997865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721749464.8U Expired - Fee Related CN207685302U (en) | 2017-12-15 | 2017-12-15 | A kind of elution pipe and washing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207685302U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659476A (en) * | 2019-03-09 | 2020-09-15 | 株式会社岛津制作所 | Handling tube and device comprising said handling tube |
| CN113186098A (en) * | 2021-05-28 | 2021-07-30 | 宁波康程德诺生物医药有限公司 | Integrated lower opening nucleic acid quick-extraction test tube, quick-extraction detection device and method |
-
2017
- 2017-12-15 CN CN201721749464.8U patent/CN207685302U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659476A (en) * | 2019-03-09 | 2020-09-15 | 株式会社岛津制作所 | Handling tube and device comprising said handling tube |
| CN113186098A (en) * | 2021-05-28 | 2021-07-30 | 宁波康程德诺生物医药有限公司 | Integrated lower opening nucleic acid quick-extraction test tube, quick-extraction detection device and method |
| CN113186098B (en) * | 2021-05-28 | 2024-03-26 | 宁波康程德诺生物医药有限公司 | Integrated lower-opening nucleic acid quick-lifting test tube, quick-lifting detection device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180803 Termination date: 20201215 |
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| CF01 | Termination of patent right due to non-payment of annual fee |