CN205635560U - A consumptive material reaches and allies oneself with consumptive material more for nucleic acid extraction - Google Patents
A consumptive material reaches and allies oneself with consumptive material more for nucleic acid extraction Download PDFInfo
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- CN205635560U CN205635560U CN201620294729.9U CN201620294729U CN205635560U CN 205635560 U CN205635560 U CN 205635560U CN 201620294729 U CN201620294729 U CN 201620294729U CN 205635560 U CN205635560 U CN 205635560U
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Abstract
The utility model provides a consumptive material reaches and allies oneself with consumptive material more for nucleic acid extraction, the consumptive material is used for required reagent of splendid attire nucleic acid extraction and blood specimen, the consumptive material is including the cracking container, washing container and the elution container that are the inline, cracking container, washing container and elution container formula structure as an organic whole. Cracking container, washing container and elution container are used for splendid attire lysate and blood specimen, washing liquid, eluate respectively, and the consumptive material needn't frequently be changed to the consumptive material of integral type when using, and steps such as schizolysis, washing, elution, convenient to use can be accomplished to a consumptive material to nucleic acid extraction's efficiency has been improved. The utility model provides an ally oneself with the consumptive material more and form side by side by a plurality of foretell consumptive material integral types, ally oneself with the consumptive material more and be convenient for carry out nucleic acid extraction to a plurality of blood specimens simultaneously.
Description
Technical field
This utility model relates to biological technical field, more particularly, to a kind of consumptive material for nucleic acid extraction and multi-joint consumptive material.
Background technology
Nucleic acid includes two kinds of molecules of DNA, RNA, biotechnology with nucleic acid as object of study, including to the extraction of nucleic acid, clone, expand, detect, a series of technology such as order-checking, develop rapidly in recent years, these technology the most not only use in research unit, and the most multiple functional department the most extensively applies.And the first step of nucleic acids research, it is simply that extracting nucleic acid from various biological samples, the efficiency of nucleic acid extraction becomes the restraining factors of downstream nucleic acid research success.Nucleic acid is all to exist with the state with protein bound in cell, mainly comprising the following steps of nucleic acid extraction: the impurity such as cell lysis removes the protein and the biomacromolecule such as polysaccharide, lipid being combined with nucleic acid, and purification of nucleic acid, removes salt, organic agent.
Existing nucleic acid extraction substantially uses paramagnetic particle method, uses nanotechnology to improve the surface of superparamagnetic nano particle and after the modification of surface, is prepared as superparamagnetism silicon oxide nanometer magnetic bead.This magnetic bead can specifically identify with nucleic acid molecules on micro interface and efficiently be combined.Utilize the superparamagnetism of silicon oxide Nano microsphere, under the effect of Chaotropic salt (guanidine hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field, can separate by DNA and RNA from blood, animal tissue, food, pathogenic microorganism equal samples, can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, disease control detection, nucleic acid samples build the multiple fields such as storehouse, environmental microorganism detection, food safety detection, molecular biology research.
Existing nucleic acid-extracting apparatus, its reaction consumptive material is for containing lysate, cleaning mixture and the eluent needed for reaction, and different steps needs to use different reaction consumptive materials, uses inconvenience, causes nucleic acid extraction efficiency low.
Utility model content
This utility model is intended to solve above-mentioned technical problem the most to a certain extent.
Primary and foremost purpose of the present utility model is to overcome the awkward defect described in above-mentioned prior art, it is provided that a kind of consumptive material for nucleic acid extraction easy to use.
Further object of the present utility model is to provide a kind of multi-joint consumptive material for nucleic acid extraction easy to use.
For solving above-mentioned technical problem, the technical solution of the utility model is as follows:
For solving above-mentioned technical problem, the technical solution of the utility model is as follows:
A kind of consumptive material for nucleic acid extraction, for containing the reagent needed for nucleic acid extraction and blood sample, described consumptive material includes that cracking container, washing container and the elution container being arranged in a linear, described cracking container, washing container and elution container are integral type structure.
In the preferred scheme of one, the number of described washing container is multiple, and it is arranged in a linear.The magnetic bead that multiple washing containers are easy to being adsorbed with nucleic acid repeatedly washs, to reduce impurity content as far as possible.
In the preferred scheme of one, described consumptive material also includes the separation sleeve container for holding separation sleeve.
In the preferred scheme of one, described consumptive material also includes that eluting pipe, eluting pipe are placed in elution container.Eluting pipe is separable with elution container, has extracted rear nucleic acid and has existed with in eluting pipe, when detecting the nucleic acid extracted further, taken out by eluting pipe from elution container, without mobile whole consumptive material.
A kind of multi-joint consumptive material for nucleic acid extraction, described multi-joint consumptive material is formed side by side by some above-mentioned consumptive material integral types.
This utility model is in use, cracking container adds lysate and magnetic bead, nucleic acid-extracting apparatus takes out separation sleeve by gear shaft from separation sleeve container, separation sleeve is socketed in gear shaft bottom, separation sleeve is moved to crack in container by gear shaft, gear shaft drives separation sleeve to be stirred, and after blood sample cracking, nucleic acid absorption is on magnetic bead, and the core of gear shaft is i.e. absorption magnetic bead in the set tip of separation sleeve after inserting bar magnet；Transfer to magnetic bead successively the first washing container, the second washing container, the 3rd washing container wash；Finally transferring to magnetic bead carry out eluting in the first elution container or eluting pipe is nucleic acid and Beads enrichment, can complete the extraction of nucleic acid.
Compared with prior art, technical solutions of the utility model provide the benefit that: this utility model provides a kind of consumptive material for nucleic acid extraction, for containing the reagent needed for nucleic acid extraction and blood sample, described consumptive material includes that cracking container, washing container and the elution container being arranged in a linear, described cracking container, washing container and elution container are integral type structure.Cracking container, washing container and elution container are respectively used to contain lysate and blood sample, cleaning mixture and eluent, the consumptive material of integral type need not frequently change consumptive material in use, one piece of consumptive material can complete the steps such as cracking, washing, eluting, it is simple to uses, thus improves the efficiency of nucleic acid extraction.
This utility model also provides for a kind of multi-joint consumptive material for nucleic acid extraction, and multi-joint consumptive material is formed side by side by some above-mentioned consumptive material integral types, and multi-joint consumptive material is easy to multiple blood samples be carried out nucleic acid extraction simultaneously.
Accompanying drawing explanation
Fig. 1 is this utility model structural representation for the consumptive material of nucleic acid extraction.
Fig. 2 is the schematic diagram of tetrad consumptive material in embodiment 2.
Wherein: 1, cracking container；2, separation sleeve container；3, the first washing container；4, the second washing container；5, the 3rd washing container；6, the first elution container；7, the second elution container；8, separation sleeve；9, eluting pipe.
Detailed description of the invention
Accompanying drawing being merely cited for property explanation, it is impossible to be interpreted as the restriction to this patent；
In order to the present embodiment is more preferably described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product；
To those skilled in the art, in accompanying drawing, some known features and explanation thereof may will be understood by omission.
With embodiment, the technical solution of the utility model is described further below in conjunction with the accompanying drawings.
Embodiment 1
As shown in Figure 1, a kind of consumptive material for nucleic acid extraction, for containing the reagent needed for nucleic acid extraction and blood sample, described consumptive material includes cracking container 1, separation sleeve container the 2, first washing container the 3, second washing container the 4, the 3rd washing container the 5, first elution container 6 and the second elution container 7 being arranged in a linear, and above-mentioned each container is integral type structure.
Cracking container 1 is used for holding lysate and blood sample, and separation sleeve container 2 is used for holding separation sleeve 8.
First washing container the 3, second washing container 4 and the 3rd washing container 5 are used to hold cleaning mixture, and the magnetic bead that multiple washing containers are easy to being adsorbed with nucleic acid repeatedly washs, to reduce impurity content as far as possible.
First elution container 6 and the second elution container 7 in use optional one, when selecting the first elution container 6, needs to add wherein eluent；When selecting the second elution container 7, put into eluting pipe 9 wherein, eluting pipe 9 adds eluent, eluting pipe 9 is separable with the second elution container 7, after extraction completes, nucleic acid exists and in eluting pipe 9, when the nucleic acid extracted is detected further, eluting pipe 9 is taken out from the second elution container 7, without mobile whole consumptive material.
This utility model is in use, cracking container 1 adds lysate and magnetic bead, nucleic acid-extracting apparatus takes out separation sleeve 8 by gear shaft from separation sleeve container 2, separation sleeve 8 is socketed in gear shaft bottom, separation sleeve 8 is moved to crack in container 1 by gear shaft, gear shaft drives separation sleeve 8 to be stirred, and after blood sample cracking, nucleic acid absorption is on magnetic bead, and the core of gear shaft is i.e. absorption magnetic bead in the set tip of separation sleeve 8 after inserting bar magnet；Transfer to magnetic bead successively first washing container the 3, second washing container the 4, the 3rd washing container 5 washs；Finally transferring to magnetic bead carry out eluting in the first elution container 6 or eluting pipe 9 is nucleic acid and Beads enrichment, can complete the extraction of nucleic acid.
This utility model provides a kind of consumptive material for nucleic acid extraction, for containing the reagent needed for nucleic acid extraction and blood sample, the consumptive material of integral type need not frequently change consumptive material in use, and one piece of consumptive material can complete the steps such as cracking, washing, eluting, it is easy to use, thus improves the efficiency of nucleic acid extraction.
Embodiment 2
The present embodiment provides a kind of multi-joint consumptive material for nucleic acid extraction, and multi-joint consumptive material is formed side by side by the consumptive material integral type described in some embodiments 1, and multi-joint consumptive material is easy to multiple blood samples be carried out nucleic acid extraction simultaneously.Fig. 2 is the schematic diagram of tetrad consumptive material, and tetrad consumptive material forms side by side by using 4 above-mentioned consumptive material integral types.
Multi-joint consumptive material is easy to carry out multiple blood samples nucleic acid extraction simultaneously, in actual applications, extracts the blood sample number of nucleic acid as required, is combined multi-joint consumptive material.Such as, extract nucleic acid if desired for 6 blood samples, then use 1 tetrad consumptive material and 2 common consumptive materials side by side；Extract nucleic acid if desired for 8 blood samples, then use 2 tetrad consumptive materials side by side, the waste that it also avoid consumptive material the most easy to use.
Common consumptive material and multi-joint consumptive material all can be with pre-packaged reagent, will lysate, cleaning mixture, eluent, magnetic bead and separation sleeve be previously added in consumptive material, eluting pipe 9 can be put and also can not be placed in consumptive material, by the mode of heating, aluminum film is sealed up whole container mouths of pipe afterwards, during use, aluminum film is torn or pierces through, can test, reduce toward the loading time in consumptive material, improve extraction efficiency.When using pre-packaged, any number of blood sample is all without waste reagent and consumptive material, and this is also the bright spot of this consumptive material.During pre-packaged reagent, magnetic bead can leave in arbitrary hole of three washing containers 602.
The corresponding same or analogous parts of same or analogous label；
Term the being merely cited for property explanation of position relationship described in accompanying drawing, it is impossible to be interpreted as the restriction to this patent；
Obviously, above-described embodiment of the present utility model is only for clearly demonstrating this utility model example, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.All any amendment, equivalent and improvement etc. made within spirit of the present utility model and principle, within should be included in this utility model scope of the claims.
Claims (5)
1. the consumptive material for nucleic acid extraction, for containing the reagent needed for nucleic acid extraction and blood sample, it is characterized in that, described consumptive material includes that cracking container, washing container and the elution container being arranged in a linear, described cracking container, washing container and elution container are integral type structure.
Consumptive material for nucleic acid extraction the most according to claim 1, it is characterised in that the number of described washing container is multiple, and it is arranged in a linear.
Consumptive material for nucleic acid extraction the most according to claim 1, it is characterised in that described consumptive material also includes the separation sleeve container for holding separation sleeve.
Consumptive material for nucleic acid extraction the most according to claim 1, it is characterised in that described consumptive material also includes that eluting pipe, eluting pipe are placed in elution container.
5. the multi-joint consumptive material for nucleic acid extraction, it is characterised in that described multi-joint consumptive material is formed side by side by the consumptive material integral type described in some claim 1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620294729.9U CN205635560U (en) | 2016-04-11 | 2016-04-11 | A consumptive material reaches and allies oneself with consumptive material more for nucleic acid extraction |
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| CN201620294729.9U CN205635560U (en) | 2016-04-11 | 2016-04-11 | A consumptive material reaches and allies oneself with consumptive material more for nucleic acid extraction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
-
2016
- 2016-04-11 CN CN201620294729.9U patent/CN205635560U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
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