CN103695419B - A kind of Viral nucleic acid extraction reagent - Google Patents
A kind of Viral nucleic acid extraction reagent Download PDFInfo
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- CN103695419B CN103695419B CN201310745655.7A CN201310745655A CN103695419B CN 103695419 B CN103695419 B CN 103695419B CN 201310745655 A CN201310745655 A CN 201310745655A CN 103695419 B CN103695419 B CN 103695419B
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- nucleic acid
- reagent
- biotin labeled
- probe
- specific probe
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 83
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 82
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 47
- 230000003612 virological effect Effects 0.000 title claims abstract description 31
- 238000000605 extraction Methods 0.000 title claims abstract description 30
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 122
- 239000000523 sample Substances 0.000 claims abstract description 109
- 239000011616 biotin Substances 0.000 claims abstract description 61
- 229960002685 biotin Drugs 0.000 claims abstract description 61
- 235000020958 biotin Nutrition 0.000 claims abstract description 61
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- 230000005291 magnetic effect Effects 0.000 claims description 70
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- 108010090804 Streptavidin Proteins 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
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- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
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- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
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- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
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- 229960004198 guanidine Drugs 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
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- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
- 238000007886 magnetic bead extraction Methods 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
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CN201310745655.7A CN103695419B (en) | 2013-12-30 | 2013-12-30 | A kind of Viral nucleic acid extraction reagent |
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CN201310745655.7A CN103695419B (en) | 2013-12-30 | 2013-12-30 | A kind of Viral nucleic acid extraction reagent |
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CN103695419A CN103695419A (en) | 2014-04-02 |
CN103695419B true CN103695419B (en) | 2016-10-12 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104974999A (en) * | 2015-04-02 | 2015-10-14 | 武汉格蓝丽富科技有限公司 | Controllable nucleic acid extraction method |
WO2016154992A1 (en) * | 2015-04-02 | 2016-10-06 | 武汉格蓝丽富科技有限公司 | Controllable method for extracting nucleic acids |
CN107022543A (en) * | 2017-05-25 | 2017-08-08 | 郑州市第六人民医院 | One kind extracts hbv nucleic acid with magnetic bead, extracts reagent, extracting method, quantitative detection kit |
CN117844986A (en) * | 2024-02-04 | 2024-04-09 | 广州维伯鑫生物科技有限公司 | Nucleic acid composition, kit and method for detecting abalone herpesvirus |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1661098A (en) * | 2004-12-29 | 2005-08-31 | 深圳益生堂生物企业有限公司 | Chop for diagnosing absence type gene of alpha thalassemia |
CN101597653A (en) * | 2009-04-22 | 2009-12-09 | 亚能生物技术(深圳)有限公司 | Fluorescence quantitative PCR detection kit for hepatitis B virus |
CN102816871A (en) * | 2012-09-10 | 2012-12-12 | 广州达健生物科技有限公司 | Fluorescence quantitation PCR (Polymerase Chain Reaction) detection kit for hepatitis c viruses (HCV) |
-
2013
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1661098A (en) * | 2004-12-29 | 2005-08-31 | 深圳益生堂生物企业有限公司 | Chop for diagnosing absence type gene of alpha thalassemia |
CN101597653A (en) * | 2009-04-22 | 2009-12-09 | 亚能生物技术(深圳)有限公司 | Fluorescence quantitative PCR detection kit for hepatitis B virus |
CN102816871A (en) * | 2012-09-10 | 2012-12-12 | 广州达健生物科技有限公司 | Fluorescence quantitation PCR (Polymerase Chain Reaction) detection kit for hepatitis c viruses (HCV) |
Non-Patent Citations (1)
Title |
---|
Genital Herpes Simplex Virus Lesions in HIV-1–Infected Men FREE;Timothy Schacker, MD et al;《JAMA》;19981231;第280卷(第1期);HIV—1 RNA Polymerase Chain Reaction and Culture部分 * |
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Owner name: HUNAN SHENGXIANG BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: HUNAN SHENGWEIER MEDICAL INSPECTION CO., LTD. Effective date: 20150512 |
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Effective date of registration: 20150512 Address after: 410012, No. 680, Lu Song Road, Changsha hi tech Development Zone, Hunan Applicant after: Sansure Biotech Inc. Address before: 410012, No. 680, Lu Song Road, Changsha hi tech Development Zone, Hunan Applicant before: Hunan company limited of Sheng Weier medical test institute |
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Denomination of invention: Viral nucleic acid extraction reagent Effective date of registration: 20170711 Granted publication date: 20161012 Pledgee: Ningbo free trade zone Terry with equity investment partnership (limited partnership)|Suzhou equity equity investment center (limited partnership)|Ningbo Meishan Bonded Port District, Jun and equity investment partnership (limited partnership)|Chen Bang Pledgor: Hunan Gene Technology Co.|Hunan San Xiang Biological Technology Co. Ltd. Registration number: 2017430000042 |
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Address after: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan Patentee after: Shengxiang Biotechnology Co., Ltd Address before: 410012, No. 680, Lu Song Road, Changsha hi tech Development Zone, Hunan Patentee before: Sansure Biotech Inc. |
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Date of cancellation: 20200217 Granted publication date: 20161012 Pledgee: Suzhou Lirui equity investment center (limited partnership)|Triton equity investment partnership (limited partnership)|JUNHE Tongrui equity investment partnership (limited partnership)|Chenbang Pledgor: SANSURE BIOTECH Inc. Registration number: 2017430000042 |