CN207650106U - Mating plate illumination imaging device - Google Patents
Mating plate illumination imaging device Download PDFInfo
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- CN207650106U CN207650106U CN201721771076.XU CN201721771076U CN207650106U CN 207650106 U CN207650106 U CN 207650106U CN 201721771076 U CN201721771076 U CN 201721771076U CN 207650106 U CN207650106 U CN 207650106U
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- illumination
- lens
- mating plate
- mirror
- imaging device
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Abstract
The utility model is related to a kind of mating plate illumination imaging devices to include by object side to image side：First achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage, image-forming objective lens and imaging len, first achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror are located in same optical axis, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage are located in same optical axis, and sample stage, image-forming objective lens and imaging len are located in same optical axis.Above-mentioned mating plate illumination imaging device combines mating reaction by one group of lenticule group and other, illumination mating plate can be made still to form limitation lamella optical illumination on illumination direction, and directional light can be become to non-parallel cross light, after illumination objective lens, the shadow region caused by all directions irradiation blocking and absorption of the not high structure of transparency from the sample on sample stage, to eliminate blocking and absorb the shade formed.
Description
Technical field
The utility model belongs to mating plate illumination imaging technique, more particularly to a kind of mating plate illumination imaging device.
Background technology
Mating plate illumination imaging (light sheet illumination) technology, is also named selection flat illumination imaging
(selective plan illumination), be it is a kind of in life science, carry out larger tissue block, plant and
Model organism (such as zebra fish, nematode) carries out the mode of fluorescence imaging.Mating plate imaging is exactly by that illumination light will be excited to hang down
Directly in the direction of imaging (rather than wide field or co-focusing imaging excite like that be imaged it is coaxial) irradiating sample as thin slice
Specific level, and the fluorescence that the level excites is imaged.Mating plate be imaged on progress it is big/thick tissue, embryo, model animal and
When plant is imaged, there are a variety of advantages compared to more traditional co-focusing imaging：1. lower fluorescent bleach；2. lower sample damage
Wound；3. high speed high s/n ratio imaging can be carried out；4. being not necessarily to pin hole, therefore there is higher sensitivity, and may be implemented more
The sample of angle is imaged.
Current mating plate imaging system is all by being expanded to the laser that laser generates.Laser beam after expanding is logical
More than half cylindrical mirrors directly become linear laser (mating plate), and project the back focal plane of illumination objective lens or laser beam pass through it is saturating
Microscope group becomes small spot and projects objective lens ', is repeatedly scanned with, is formed virtual in one direction by scanning galvanometer
Mating plate.Side irradiating sample of the mating plate that both the above mode is formed from sample.Due to mating plate presence and from side irradiate,
Sample is confined to very thin one layer by the range of laser irradiation, forms natural lamella effect.It is glimmering in laser excitation sample
The fluorescence that signal obtains by the collection shots vertical with irradiation laser direction and is imaged.
Unilateral illumination light path, for the side of no illumination, the scattering to exciting light and refraction action due to sample can cause
Fluorescent brightness without the excitation of illumination side declines, and background can be become by scattering light activated fluorescence, makes sample imaging fluorescent brightness not
, signal-to-noise ratio declines.In order to make up and compensate the subproblem of unilateral illumination, the illumination of generally use bilateral.Increase in offside
A set of identical light path, makes sample other side also directly be excited by sample.Bilateral lighting engineering can change to a certain extent
Kind brightness of image unevenness and shadow problem, but can not be fully solved.
Utility model content
Based on this, it is necessary to provide a kind of mating plate illumination imaging device that can be eliminated blocking and absorb the shade formed.
A kind of mating plate illumination imaging device includes by object side to image side：First achromatic lens, the second achromatic lens,
Semicylinder mirror, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage, image-forming objective lens and imaging len, institute
It states the first achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror to be located in same optical axis, the reflective mirror, third
Achromatic lens, lenticule group, illumination objective lens, sample stage are located in same optical axis, and the sample stage, image-forming objective lens and imaging are saturating
Mirror is located in same optical axis.
The lenticule group is cylindrical microlenses group in one of the embodiments,.
The side of the cylindrical microlenses group is curved surface in one of the embodiments,.
The illumination objective lens are 4X, 10X or 20X object lens in one of the embodiments,.
The image-forming objective lens are 10X, 20X, 40X or 60X water immersion objective in one of the embodiments,.
First achromatic lens is lens in one of the embodiments,.
Second achromatic lens is lens in one of the embodiments,.
The semicylinder mirror is semicylinder mirror in one of the embodiments,.
The third achromatic lens is lens in one of the embodiments,.
The illumination path of the mating plate illumination imaging device is bilateral illumination path or unilateral side in one of the embodiments,
Lighting circuit.
Above-mentioned mating plate illumination imaging device includes by object side to image side：First achromatic lens, the second achromatic lens, half
Cylindrical mirror, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage, image-forming objective lens and imaging len, it is described
First achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror are located in same optical axis, and the reflective mirror, third disappear
Aberration lens, lenticule group, illumination objective lens, sample stage are located in same optical axis, the sample stage, image-forming objective lens and imaging len
In same optical axis.The specific region illumination that sample is carried out using direct mating plate lighting engineering, carries out mating plate imaging, direct
Mating plate enters before objective lens ', combines mating reaction by one group of lenticule group and other, can make illumination mating plate
Limitation lamella optical illumination is still formed on illumination direction, and the nearly directional light for forming light sheets can be become non-parallel intersection
Light, after illumination objective lens, the high refraction of all directions irradiation, the resistance of the not high structure of transparency from the sample on sample stage
Shadow region caused by gear and absorption, to eliminate blocking and absorb the shade formed.
Description of the drawings
Fig. 1 is the structural scheme of mechanism of the mating plate illumination imaging device of an embodiment；
Fig. 2 is the schematic top plan view of the lenticule group of an embodiment；
Fig. 3 is the schematic side view of the lenticule group of an embodiment；
Fig. 4 is the image-forming objective lens focal plane structure top view of the mating plate illumination imaging device of an embodiment；
Fig. 5 is the image-forming objective lens focal plane structural side view of the mating plate illumination imaging device of an embodiment.
Specific implementation mode
The utility model is described more fully with below with reference to attached drawing, shows the exemplary of the utility model in the accompanying drawings
Embodiment.As those skilled in the art will appreciate that, described embodiment can repair in a variety of ways
Change, spirit or scope of all modifications without departure from the utility model.
Referring to Fig. 1, the mating plate illumination imaging device 100 of an embodiment includes that the first achromatism is saturating by object side to image side
Mirror 101, the second achromatic lens 102, semicylinder mirror 103, reflective mirror 104, third achromatic lens 105, lenticule group 106,
Illumination objective lens 107, sample stage 108, image-forming objective lens 109 and imaging len 110.
First achromatic lens 101, the second achromatic lens 102, semicylinder mirror 103, reflective mirror 104 are located at same optical axis
On, light beam forms primary optic axis through said elements.Reflective mirror 104, third achromatic lens 105, lenticule group 106, lighting
Mirror 107, sample stage 108 are located in same optical axis, and light beam forms the second optical axis through said elements.Sample stage 108, image-forming objective lens
109 and imaging len 110 be located in same optical axis, light beam through said elements formed third optical axis.Primary optic axis and the second optical axis
Substantially vertical, the second optical axis is substantially vertical with third optical axis, and primary optic axis and third optical axis are coaxial.
The light velocity that laser is sent out is after the first achromatic lens 101, the second achromatic lens 102, through semicylinder mirror
After 103 refractions, under the effect of reflective mirror 104, beam course is adjusted, light beam is vertical with original direction, saturating through third achromatism
Mirror 105, under the action of lenticule group 106, light beam still can form mating plate in the position perpendicular to image-forming objective lens focal plane,
But in focal plane direction, irradiation light is non-parallel cross light, light beam thin slice is formed, to excite the fluorescence of sample, light beam to arrive
Up to after the biological sample on sample stage 108, beam direction changes, and with original direction substantially at 90 degree, then image-forming objective lens 109 are received
The fluorescence signal for collecting biological sample obtains imaging results after imaging len 110 through camera detection fluorescence signal.
In one embodiment, the first achromatic lens 101 is lens, preferably F25 lens.
In one embodiment, the second achromatic lens 102 is lens, preferably F75 lens.
In one embodiment, semicylinder mirror 103 is semicylinder mirror, preferably F50, F75 semicylinder mirror.
In one embodiment, third achromatic lens 105 is lens, preferably F25 lens.
Fig. 2 and 3 are please referred to, in one embodiment, lenticule group 106 is cylindrical microlenses group.Specifically, implement one
In mode, the side of cylindrical microlenses group is curved surface.The focal length of different semi-cylindrical lens and different spacing can be in various degree
Shorten shadow length.As can be seen that mating plate still can be formed in the position perpendicular to image-forming objective lens focal plane from Figure 4 and 5,
But in focal plane direction, irradiation light is non-parallel cross light.
In one embodiment, illumination objective lens 107 are 4X, 10X or 20X object lens.Specifically, in one embodiment, it shines
Bright object lens 107 are 4X, 10X or 20X Nikon or Olympus object lens.
In one embodiment, sample stage 108 is for holding biological sample.For immobilizing biological samples, and make biological sample
Product three-dimensional is moved and can planar be rotated, and to adjust the position of biological sample, and carries out longitudinal scanning to sample in imaging,
To obtain three-dimensional fluorescent image.
In one embodiment, image-forming objective lens 109 are 10X, 20X, 40X or 60X water immersion objectives.Specifically, implement one
In mode, image-forming objective lens 109 are 10X, 20X, 40X or 60X Nikon or Olympus water immersion objective.
In one embodiment, imaging len 110 uses the imaging len for corresponding to object lens producer.
Preferably, in one embodiment, as shown in Figure 1.Mating plate illumination imaging device is bilateral illumination path.Direct
On the basis of mating plate illumination, same a set of light path is built again in other side so that sample both sides are carried out at the same time mating plate illumination and swash
Hair.Using bilateral illumination path, eliminates blocking and absorb the better of the shade formed.In other embodiments, can also
Using unilateral illumination light path.
Above-mentioned mating plate illumination imaging device includes by object side to image side：First achromatic lens, the second achromatic lens, half
Cylindrical mirror, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage, image-forming objective lens and imaging len, it is described
First achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror are located in same optical axis, and the reflective mirror, third disappear
Aberration lens, lenticule group, illumination objective lens, sample stage are located in same optical axis, the sample stage, image-forming objective lens and imaging len
In same optical axis.The restricted area illumination that sample is carried out using direct mating plate lighting engineering, carries out mating plate imaging, direct
Mating plate enters before objective lens ', combines mating reaction by one group of lenticule group and other, can make illumination mating plate
Limitation lamella optical illumination is still formed on illumination direction, and directional light can be become to non-parallel cross one another light, using
It is cloudy caused by all directions irradiation blocking and absorption of the not high structure of transparency from the sample on sample stage after illumination objective lens
Shadow zone domain, to eliminate blocking and absorb the shade formed.
Each technical characteristic of embodiment described above can be combined arbitrarily, to keep description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (10)
1. a kind of mating plate illumination imaging device, which is characterized in that include by object side to image side：First achromatic lens, second disappear
Aberration lens, semicylinder mirror, reflective mirror, third achromatic lens, lenticule group, illumination objective lens, sample stage, image-forming objective lens and at
As lens, first achromatic lens, the second achromatic lens, semicylinder mirror, reflective mirror are located in same optical axis, described anti-
Light microscopic, third achromatic lens, lenticule group, illumination objective lens, sample stage are located in same optical axis, the sample stage, imaging object
Mirror and imaging len are located in same optical axis.
2. mating plate illumination imaging device according to claim 1, which is characterized in that the lenticule group is cylindrical microlenses
Group.
3. mating plate illumination imaging device according to claim 2, which is characterized in that the side of the cylindrical microlenses group is
Curved surface.
4. mating plate illumination imaging device according to claim 1, which is characterized in that the illumination objective lens be 4X, 10X or
20X object lens.
5. mating plate illumination imaging device according to claim 1, which is characterized in that the image-forming objective lens be 10X, 20X,
40X or 60X water immersion objectives.
6. mating plate illumination imaging device according to claim 1, which is characterized in that first achromatic lens is
Mirror.
7. mating plate illumination imaging device according to claim 1, which is characterized in that second achromatic lens is
Mirror.
8. mating plate illumination imaging device according to claim 1, which is characterized in that the semicylinder mirror is semicylinder mirror.
9. mating plate illumination imaging device according to claim 1, which is characterized in that the third achromatic lens is
Mirror.
10. mating plate illumination imaging device according to claim 1, which is characterized in that the mating plate illumination imaging device
Illumination path is bilateral illumination path or unilateral illumination circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721771076.XU CN207650106U (en) | 2017-12-18 | 2017-12-18 | Mating plate illumination imaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721771076.XU CN207650106U (en) | 2017-12-18 | 2017-12-18 | Mating plate illumination imaging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207650106U true CN207650106U (en) | 2018-07-24 |
Family
ID=62877494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721771076.XU Expired - Fee Related CN207650106U (en) | 2017-12-18 | 2017-12-18 | Mating plate illumination imaging device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207650106U (en) |
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2017
- 2017-12-18 CN CN201721771076.XU patent/CN207650106U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180724 Termination date: 20191218 |
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| CF01 | Termination of patent right due to non-payment of annual fee |