CN207627441U - The sequential constant temperature perfusion slaking apparatus of liver - Google Patents
The sequential constant temperature perfusion slaking apparatus of liver Download PDFInfo
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- CN207627441U CN207627441U CN201720189979.0U CN201720189979U CN207627441U CN 207627441 U CN207627441 U CN 207627441U CN 201720189979 U CN201720189979 U CN 201720189979U CN 207627441 U CN207627441 U CN 207627441U
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- perfusion
- constant temperature
- liver
- digestion
- outlet
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Abstract
The utility model is related to a kind of sequential constant temperature perfusion slaking apparatus of liver, including syringe, constant flow pump, heat-exchange device, three-way pipe, portal vein perfusion catheter, digestive shelf, drainage pumps.The utility model can carry out the big animal's liver such as pig In situ perfusion method and in vitro digestion, perfusion success rate can be improved, realizes continual continuous perfusion digestion, while the preferable temperature that ensure that in perfusion and digestion process, digestive efficiency is improved, digestion time is shortened.Higher cell yield and better cell viability can be obtained, strong platform is provided for hepatocyte transplantation.
Description
Technical field
The utility model is related to medical field, specifically a kind of sequential constant temperature perfusion slaking apparatus of liver.
Background technology
Hepatocyte transplantation is that the explosive liver for the treatment of declines the important means of equal liver diseases, but due to for liver shortage etc.,
So that liver cell source is limited.The liver cell of pig source property is the important cells source of xenogenesis liver cell therapy.Some researches show that
The liver cell of pig source property can play biological function in receptor body.After simultaneously can be by carrying out genetic modification to pig, carry
Porcine hepatocyte is taken, rejection can be mitigated.
The separate mode used at present is four step perfusion digestion methods being perfused with IV Collagenase Types as core.But due to behaviour
Be related to a variety of perfusion liquids as process, operating process is complicated, changes liquid process and easy tos produce bubble, while be perfused and digestion time compared with
It is long, it effectively whole can not ensure stationary temperature, be unfavorable for the digestion separation of cell.There is research to confirm, digestion time and liver
The yield and cell viability of cell are negatively correlated.
The liver volume of big animal is larger, cannot be perfused using manual injection, rely primarily on charge pump and be irrigated.Due to
The injection pressure of charge pump is relatively large, and common silk thread ligation easily causes the de- pipe phenomenon of perfusion tube.
Utility model content
The purpose of this utility model is to provide a kind of sequential constant temperature perfusion slaking apparatus of liver, and it is quiet to solve door in the prior art
Arteries and veins intubation is fixed, and perfusion liquid changes liquid process, temperature persistent problem in perfusion and digestion process, and extraction pork liver that can be more efficient is thin
Born of the same parents meet the needs of hepatocyte transplantation.
To achieve the above object, the utility model uses following technical scheme：
A kind of sequential constant temperature perfusion slaking apparatus of liver, including constant flow pump and digestive shelf；
The constant flow pump is provided with multiple channels, and each channel is separately connected a syringe, and multiple syringes pass through
Connector is connect with portal vein perfusion catheter, and portal vein perfusion catheter is heated by heat-exchange device；Portal vein perfusion catheter is another
End is stretched into digestive shelf for carrying out In situ perfusion method and in vitro digestion；
The digestive shelf is hollow groove body, has recirculated water for providing isoperibol for digestive shelf in groove body, disappears
Change and be provided with digestion strainer in the groove of slot, digests on strainer for placing liver, digestion capping is provided on digestive shelf.
The constant flow pump includes an interface, and four interfaces are separately connected a syringe；The outlet of two of which syringe
Connected by the first three-way pipe, the outlet of other two syringe connect by the second three-way pipe, the outlet of the first three-way pipe and
The outlet of second three-way pipe is connected by third three-way pipe；The outlet of third three-way pipe is connect with portal vein perfusion catheter.
The liquid outlet of the syringe is respectively mounted sterilizing filter membrane.
The heat-exchange device includes the thermostat water bath with heat exchange inner core, and portal vein perfusion catheter one end twines
It is wound on heat exchange inner core.
The portal vein perfusion catheter is used to be perfused one end with the slot line structure that two segment length are 1cm, between two sections of line of rabbet joint
Every 1cm.
Inlet is arranged in digestive shelf groove body upper end, and liquid outlet is arranged in lower end, and inlet and liquid outlet carry cut-off
Valve；There are conduction hole, conduction holes to import waste liquid cylinder through drainage tube for bottom portion of groove；Liquid outlet flows into thermostat water bath by the road, permanent
The inlet that warm water bath is connected to digestive shelf upper end through hot recycle pump forms circulating water loop.
The digestion strainer is 200 mesh hypocrateriform metal meshes, and shape matches with groove structure.
Multiple slots for placing centrifuge tube are provided on the digestive shelf groove body.
Compared with the existing technology, the utility model has the following advantages：
The utility model first uses sequential device, and the tight that different perfusion liquids can be realized only by adjusting threeway is cut
It changes, shortens infusion time and due to changing bubble formation caused by liquid；Again by the circulating-heating to perfusion catheter and digestive shelf,
It can realize perfusion and digest the constant temperature holding of overall process, improve digestive efficiency；The utility model can be to the big animal such as pig
Liver carries out In situ perfusion method and in vitro digestion, can improve perfusion success rate, realizes that continual continuous perfusion digests, while compared with
The good temperature that ensure that in perfusion and digestion process, improves digestive efficiency, shortens digestion time.It can obtain higher
Cell yield and better cell viability, strong platform is provided for hepatocyte transplantation.
Further, by using the conduit with the line of rabbet joint, intubation can be preferably fixed, prevents from rushing due to perfusion pump pressure
De- pipe phenomenon caused by hitting.
Further, the liquid outlet of described 50ml syringes A, B, C, D are respectively mounted sterilizing filter membrane, it is ensured that the sterile behaviour of whole process
Make, reduces germ contamination.
Further, the heat-exchange device includes thermostat water bath, and the heat exchanger with inner core forms；Thermostat water bath
Temperature be set as 37-41 DEG C, the flow velocity of the heat recirculated water in digestive shelf is 80-100ml/min.To ensure temperature when digestion
Spend it is constant with 37 DEG C or so.
Description of the drawings
The sequential constant temperature perfusion slaking apparatus schematic diagram of liver of Fig. 1 the utility model；
The portal vein perfusion catheter enlarged drawing of Fig. 2 the utility model；
The digestive shelf vertical view of Fig. 3 the utility model；
Wherein, 1. constant flow pump；2. syringe A；3. syringe B；4. syringe C；5. syringe D；6. sterilised membrane filter；7. nothing
Bacterium filter membrane；8. sterilised membrane filter；9. sterilised membrane filter；10. liquid flowing outlet；11. liquid flowing outlet；12. liquid flowing outlet；13. liquid
Body outflux；14. three-way pipe；15. three-way pipe；16. liquid flowing outlet；17. liquid flowing outlet；18. three-way pipe；19. liquid flow
Outlet；20. thermostat water bath；21. heat exchange inner core；22. portal vein perfusion catheter；23. digesting strainer；24. digestive shelf；25.
Inlet；26. liquid outlet；27. conduction hole；28. waste liquid cylinder；29 digestion cappings；30. slot；31. thermostat water bath；32. cycle
Pump.
Specific implementation mode
Below in conjunction with the accompanying drawings, specific embodiment of the present utility model is described in detail, but the utility model is unlimited
In the embodiment.In order to make the public have thorough understanding to the utility model, in following the utility model preferably applies example in detail
Illustrate concrete details.
Referring to Fig. 1, a kind of sequential constant temperature perfusion slaking apparatus of liver, including constant flow pump 1, constant flow pump 1 is equipped with 4 and leads to
Road：Channel one connects 50ml syringe A2, outlet connection sterilised membrane filter 6, and front end liquid flowing outlet 10 is connected through connecting conduit
To 14 one end of three-way pipe；Channel two connects 50ml syringes B 3, outlet connection sterilised membrane filter 7, and front end liquid flowing outlet 11 passes through
Connecting conduit is connected to 14 Single port of three-way pipe；Channel three connects 50ml syringes C 4, outlet connection sterilised membrane filter 8, front end
Liquid flowing outlet 12 is connected to 15 Single port of three-way pipe through connecting conduit, and channel four connects 50ml syringes D 5, outlet connection
Sterilised membrane filter 9, front end liquid flowing outlet 13 are connected to 15 a port of three-way pipe through connecting conduit.The liquid of three-way pipe 14 flows out
Mouthfuls 16, the liquid flowing outlet 17 of three-way pipe 15 is connected to two ports of three-way pipe 18 through connecting conduit, by three-way pipe 18
Liquid flowing outlet 19, connection portal vein perfusion catheter 22, perfusion catheter one ends wound is in heat exchange inner core 21, through thermostat water bath
20 are heated to 37 DEG C, and 22 leading portion of portal vein perfusion catheter has the line of rabbet joint of two sections of 1cm, and two is intersegmental every 1cm (as shown in Figure 2).Digestion
Strainer 23 is 200 mesh pallet sample metal meshes, is placed in 24 groove of digestive shelf, and digestive shelf 24 is hollow groove body, and feed liquor is arranged in upper end
Liquid outlet 26 is arranged in mouth 25, lower end, and there are the conduction holes 27 of a diameter of 0.8cm for bottom portion of groove, and waste liquid cylinder 28 is imported through drainage tube,
Digestive shelf 24 is with a digestion capping 29, four slots 30 (as shown in Figure 3) that can place 50ml centrifuge tubes.Thermostat water bath
31 are connected to the inlet 25 of 24 upper end of digestive shelf through hot recycle pump 32, and recirculated water is through 26 connecting conduit of liquid outlet to water-bath
31。
Further, the digestive shelf 24, including 200 mesh digest strainer, and the liquid capacity of groove body is 1.4L, and inlet is located at
Top, liquid outlet are located at bottom, carry shut-off valve.
Further, the sequential constant temperature perfusion slaking apparatus of the liver, inner diameter are 6mm.
Further, the temperature of the thermostat water bath is set as 37-41 DEG C, and the flow velocity of the heat recirculated water in digestive shelf is
80-100ml/min.To ensure digestion when temperature it is constant with 37 DEG C or so.
In conjunction with Fig. 1, the specific mode of operation of the utility model is as follows：
Routine disinfection surgical instrument, various components in equipment, including digestion strainer 23, digestive shelf 24, connecting conduit and door
Intravenous infusion pipe 22.Sterile assembling is kept later.Syringe is loaded into A respectively：Heparin sodium aqua, B：PBE perfusion liquids, C：PB is perfused
Liquid, D：IV Collagenase Type solution, since perfusion liquid usage amount is larger, each perfusion liquid is ready for 4 50ml syringes.According to need
Ask respectively carry out perfusion it is sequential.Using sequential device, the tight that different perfusion liquids can be realized only by adjusting threeway is cut
It changes, shortens infusion time and due to changing bubble formation caused by liquid；
The wuzhishan miniature pig for taking for 1 monthly age, after free from worries 10mg/Kg induced anesthesias, conventional median incision approach is fully sudden and violent
Reveal liver, dissociate lesser omentum, portal vein, arteria hepatica, infrahepatic vena cava, liver superior and inferior vena cava.It is positioned over the progress of digestive shelf 24
Sequential operation.
The utility model can carry out the big animal's liver such as pig In situ perfusion method and in vitro digestion, can improve and be perfused successfully
Rate realizes continual continuous perfusion digestion, while the preferable temperature that ensure that in perfusion and digestion process, improves digestion
Efficiency shortens digestion time.Higher cell yield and better cell viability can be obtained, is provided for hepatocyte transplantation
Strong platform.
More than, the only preferred embodiment of the utility model is not limited only to the scope of the utility model, Fan Yiben
The equivalence changes and modification that the content of utility model patent range is done all should be the technology scope of the utility model.
Claims (8)
1. a kind of sequential constant temperature perfusion slaking apparatus of liver, it is characterised in that：Including constant flow pump (1) and digestive shelf (24)；
The constant flow pump (1) is provided with multiple channels, and each channel is separately connected a syringe, and multiple syringes are by connecing
Head is connect with portal vein perfusion catheter (22), and portal vein perfusion catheter (22) is heated by heat-exchange device；Portal vein perfusion is led
Pipe (22) other end stretches into digestive shelf (24) for carrying out In situ perfusion method and in vitro digestion；
The digestive shelf (24) is hollow groove body, have in groove body recirculated water for be digestive shelf (24) offer constant temperature ring
Border, is provided with digestion strainer (23) in the groove of digestive shelf (24), for placing liver, digestive shelf (24) on digestion strainer (23)
On be provided with digestion capping (29).
2. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：Constant flow pump (1) packet
(4) a interface is included, four interfaces are separately connected a syringe；The outlet of two of which syringe passes through the first three-way pipe (14)
The outlet of connection, other two syringe is connected by the second three-way pipe (15), the outlet and the two or three of the first three-way pipe (14)
The outlet of siphunculus (15) is connected by third three-way pipe (18)；The outlet of third three-way pipe (18) and portal vein perfusion catheter
(22) it connects.
3. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：The syringe goes out liquid
Mouth is respectively mounted sterilizing filter membrane.
4. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：The heat-exchange device packet
The thermostat water bath (20) with heat exchange inner core (21) is included, portal vein perfusion catheter (22) one ends wound is in heat exchange
On core (21).
5. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：The portal vein perfusion is led
Pipe (22) is used to be perfused one end with the slot line structure that two segment length are 1cm, two sections of line of rabbet joint interval 1cm.
6. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：Digestive shelf (24) slot
Inlet (25) is arranged in body upper end, and liquid outlet (26) is arranged in lower end, and inlet (25) and liquid outlet (26) carry shut-off valve；It is recessed
There are conduction hole (27), conduction holes (27) to import waste liquid cylinder (28) through drainage tube for trench bottom；Liquid outlet (26) flows into perseverance by the road
Warm water bath (31), the inlet (25) that thermostat water bath (31) is connected to digestive shelf (24) upper end through hot recycle pump (32) are formed
Circulating water loop.
7. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：The digestion strainer (23)
For 200 mesh hypocrateriform metal meshes, shape matches with groove structure.
8. the sequential constant temperature perfusion slaking apparatus of liver according to claim 1, it is characterised in that：Digestive shelf (24) slot
Multiple slots (30) for placing centrifuge tube are provided on body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720189979.0U CN207627441U (en) | 2017-02-28 | 2017-02-28 | The sequential constant temperature perfusion slaking apparatus of liver |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720189979.0U CN207627441U (en) | 2017-02-28 | 2017-02-28 | The sequential constant temperature perfusion slaking apparatus of liver |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207627441U true CN207627441U (en) | 2018-07-20 |
Family
ID=62860153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720189979.0U Expired - Fee Related CN207627441U (en) | 2017-02-28 | 2017-02-28 | The sequential constant temperature perfusion slaking apparatus of liver |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207627441U (en) |
-
2017
- 2017-02-28 CN CN201720189979.0U patent/CN207627441U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180720 Termination date: 20200228 |