CN207571378U - A kind of liquid submerges high-NA microcobjective admittedly - Google Patents
A kind of liquid submerges high-NA microcobjective admittedly Download PDFInfo
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- CN207571378U CN207571378U CN201721533494.5U CN201721533494U CN207571378U CN 207571378 U CN207571378 U CN 207571378U CN 201721533494 U CN201721533494 U CN 201721533494U CN 207571378 U CN207571378 U CN 207571378U
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Abstract
The utility model discloses a kind of liquid to submerge high-NA microcobjective admittedly, microcobjective includes lens barrel and several optical elements in lens barrel, it is lens positioned at the lowermost optical element, the lower surface of lens is optical flat, the lower face of optical flat protrusion or parallel lens barrel, is sequenced sample for being directly immersed in sample solution.Since the lower face for being located at the lowermost optical element lenses is optical flat, and the lower face of optical flat protrusion or parallel lens barrel, it is sequenced so that the end with optical flat can be directly immersed in sample solution by microcobjective, not needing to the liquid-immersion device of installation auxiliary can directly be sequenced in immersion liquid, simplify the structure of microcobjective, sample solution is isolated without coverslip, improves sequencing efficiency.
Description
Technical field
The present invention relates to gene sequencing technology fields, and in particular to a kind of liquid submerges high-NA microcobjective admittedly.
Background technology
The resolving power of microcobjective is determined that numerical aperture NA=nsin θ, wherein θ are object lens imaging beam by numerical aperture
Angular aperture, angular aperture is limited by factors such as optical design, processing, adjustments cannot be excessive, so microcobjective generally uses immersion liquid
Medium refraction index n is improved to increase numerical aperture.
Liquid is as image path medium, and microcobjective needs to be designed for determining immersion liquid refractive index, and liquid is situated between
Matter is usually pure water or oil, and oily refractive index has strict demand, and in many cases, tested sample needs specific solution leaching
Not yet, such as organic-biological fluorescent material needs solution containing antioxidant to submerge protection, this kind of solution and pure water or oil
Refractive index difference is larger, and serious to reduce microcobjective image quality, the universal method for solving the problems, such as this is separated using coverslip
Open sample solution and object lens immersion liquid, it is therefore an objective to make sample solution thickness sufficiently small and relax its refractive index tolerance, and microcobjective
It needs to increase interior lens regulating mechanism to adapt to cover-glass thickness tolerance.
At present, more and more application requirement sample carriers do not use coverslip, are on the one hand to reduce microcobjective complexity
It spends and reduces cost, be on the other hand that in many cases, sample needs recycle biochemical reaction in different solutions, it is biochemical anti-every time
Should after carry out micro-imaging, the coverslip for removing sample carriers is conducive to sample biochemical reaction process is recycled in different solutions.
Such as gene sequencing biochip sample, a base of genetic fragment is measured after each biochemical reaction, to measure long enough base
Because of sequence, biochemical reaction is needed to be recycled up to a hundred times with micro-imaging, removal gene sequencing biochip sample coverslip will substantially
Degree reduces biochemical reaction complexity.This kind of application proposes new requirement to microcobjective immersion liquid design.
Invention content
The application offer is a kind of to carry out immersion liquid sequencing, and do not need to install it to the sample that no coverslip is isolated
He assists the liquid of liquid-immersion device to submerge high-NA microcobjective admittedly.
A kind of liquid in a kind of embodiment is provided and submerges high-NA microcobjective admittedly, including lens barrel and in lens barrel
Several optical elements, be lens positioned at the lowermost optical element, the lower surfaces of lens is optical flat, and optical flat is dashed forward
Go out or the lower face of parallel lens barrel, sample is sequenced for being directly immersed in sample solution.
Further, lens are planoconvex spotlight, and upper surface is convex surface.
In other embodiments, lens are plano-concave lens, and upper surface is concave surface.
In other embodiments, lens are planar lens, and upper surface is optical flat.
Further, several optical elements form infinity conjugated structure.
Further, the numerical aperture 1.0 of object lens, focal length 5mm, field number 1mm.
Further, the material of lens is fused quartz.
A kind of liquid according to above-described embodiment submerges high-NA microcobjective admittedly, due to being located at the lowermost optics member
The lower face of part lens is optical flat, and optical flat is prominent or the lower face of parallel lens barrel so that microcobjective can be direct
End with optical flat is immersed in sample solution and is sequenced, the liquid-immersion device for not needing to installation auxiliary can be direct
Immersion liquid is sequenced, and simplifies the structure of microcobjective, and sample solution is isolated without coverslip, improves sequencing efficiency.
Description of the drawings
Fig. 1 is the structure diagram that microcobjective immersion liquid is sequenced in a kind of embodiment；
Fig. 2 is the partial enlarged view of Fig. 1 end portions；
Fig. 3 is the flow chart of the sequencing approach of microcobjective in another embodiment；
Fig. 4 is the schematic diagram that planoconvex spotlight immerses sample solution in another embodiment.
Specific embodiment
The present invention is described in further detail below by specific embodiment combination attached drawing.
Embodiment one：
It present embodiments provides a kind of liquid and submerges high-NA microcobjective admittedly, it is micro- that this liquid submerges high-NA admittedly
Object lens are mostly installed on gene sequencer and use, for be directly immersed in the sample solution of no coverslip isolation to sample into
Row sequencing.
As depicted in figs. 1 and 2, the liquid of the present embodiment submerges high-NA microcobjective mainly including lens barrel (in figure admittedly
To show) and several optical elements, optical element be lens, positioned at the lowermost optical element be planoconvex spotlight 1, plano-convex
The lower surface of lens 1 is optical flat, and upper surface is convex surface, positioned at the lower surface of the lowermost planoconvex spotlight 1 (optical flat)
The lower face of parallel lens barrel can be directly immersed in so as to the lower surface (optical flat) of planoconvex spotlight 1 in sample solution 2, and sample is molten
Liquid 2 is located on sample 3.The light that planoconvex spotlight 1 converges, which is directly entered in sample solution 2, to be refracted on sample 3.In the present embodiment,
The material of planoconvex spotlight 1 is fused quartz, and fused quartz corrosion resistance is strong, can adapt to the sample solution of wider range.
Parallel light beam is formed between two optical elements in several optical elements of the present embodiment, forms infinity
Conjugated structure can meet the needs of more Multifunctional design.And several optical element combinations into object lens numerical aperture
1.0, focal length 5mm, field number 1mm.
In other embodiments, the lower surface (optical flat) of planoconvex spotlight 1 can also protrude from the lower face of lens barrel, i.e., directly
It connects and the lower surface (optical flat) of planoconvex spotlight 1 is immersed in sample solution 2.Positioned at the lowermost lens be lower surface it is light
The lens of plane and upper surface for other structures are learned, such as upper surface is concave surface, lower surface is the plano-concave lens of optical flat；Or
Person's upper and lower surface is the planar lens of optical flat.
Liquid provided in this embodiment submerges high-NA microcobjective admittedly, is flat due to being located at the lowermost optical element
Convex lens, lower face are optical flat, and the lower face of the parallel lens barrel of optical flat so that microcobjective will can directly have
The end of optical flat, which is immersed in sample solution, to be sequenced, and is not needed to the liquid-immersion device of installation auxiliary and can directly immersion liquid be surveyed
Sequence simplifies the structure of microcobjective, and sample solution is isolated without coverslip, improves sequencing efficiency.
Embodiment two：
The present embodiment provides the sequencing approach that a kind of liquid submerges high-NA microcobjective admittedly, this sequencing approach is based on upper
The microcobjective for stating embodiment one is realized.
As shown in figure 3, the sequencing approach of the present embodiment mainly includes the following steps：
S001：Object lens immerse sample solution；
It is not covered as shown in figure 4, the lower surface parallel with camera lens of planoconvex spotlight 1 (optical flat) is directly immersed in naturally
In the sample solution 2 of slide isolation, and immersion liquid thickness is that (i.e. optical flat is 0.1mm apart from sample/object lens operating distance
0.1mm)。
S002：Quick mobile sequencing.
Quick mobile example 3 and sample solution 2 are sequenced, and the optical flat of planoconvex spotlight 1 remains immersion
In sample solution 2.
The thickness (object lens operating distance) of sample solution 2 and the refractive index tolerance of sample solution 2 inversely, to put
The refractive index tolerance of wide sample solution 2 then needs to reduce immersion liquid thickness (object lens working distance as far as possible when microcobjective designs
From).Design calculates as follows：
2 refractive index tolerance Δ n of sample solution is by the numerical aperture NA of microcobjective, operating distance (the immersion liquid thickness of object lens
Mm) d, the refractive index nominal value n of sample solution, microcobjective image quality allow decline degree codetermine.Microcobjective image quality
Allow many factors such as decline degree and microcobjective spectral width, visual field, aberration characteristic related, it is impossible to it is accurate with analytic expression
Expression provides the empirical equation of multiple summary of Design：
Wherein, k is to allow the relevant coefficient of decline degree with microcobjective image quality, and empirical value is 0.02~0.004.
In the present embodiment, the numerical aperture NA of object lens is 1.0, and immersion liquid thickness d is 0.1mm, and sample solution 2 is pure water, 20
Refractive index n under DEG C environment temperature is 1.333044, by above-mentioned empirical equation, to k values 0.003, can to sample solution 2
Refractive index tolerance Δ n ranges are about ± 0.039, the biochemical reaction sample solution margin of tolerance can be common to, available for sample
It is sequenced.
A kind of liquid provided in this embodiment submerges the sequencing approach of high-NA microcobjective admittedly, will directly can have light
The end of plane, which is immersed in sample solution, to be sequenced, and is not needed to the liquid-immersion device of installation auxiliary and can directly immersion liquid be surveyed
Sequence simplifies the structure of microcobjective, and sample solution is isolated without coverslip, improves sequencing efficiency.It and can be by immersion liquid thickness
Degree design it is sufficiently small, to increase 2 refractive index tolerance of sample solution, immersion liquid is isolated without coverslip, also can guarantee pair
The rice genome sequence of sample.
Use above specific case is illustrated the present invention, is merely used to help understand the present invention, not limiting
The system present invention.For those skilled in the art, thought according to the present invention can also be made several simple
It deduces, deform or replaces.
Claims (7)
1. a kind of liquid submerges high-NA microcobjective admittedly, which is characterized in that including lens barrel and in the lens barrel
Several optical elements are lens positioned at the lowermost optical element, and the lower surfaces of the lens is optical flat, the optics
The lower face of plane protrusion or the parallel lens barrel, is sequenced sample for being directly immersed in sample solution.
2. liquid as described in claim 1 submerges high-NA microcobjective admittedly, which is characterized in that the lens are saturating for plano-convex
Mirror, upper surface are convex surface.
3. liquid as described in claim 1 submerges high-NA microcobjective admittedly, which is characterized in that the lens are saturating for plano-concave
Mirror, upper surface are concave surface.
4. liquid as described in claim 1 submerges high-NA microcobjective admittedly, which is characterized in that the lens are saturating for plane
Mirror, upper surface are optical flat.
5. as Claims 1-4 any one of them liquid submerges high-NA microcobjective admittedly, which is characterized in that several
The optical element forms infinity conjugated structure.
6. liquid as claimed in claim 5 submerges high-NA microcobjective admittedly, which is characterized in that the numerical aperture of object lens
1.0, focal length 5mm, field number 1mm.
7. as Claims 1-4 any one of them liquid submerges high-NA microcobjective admittedly, which is characterized in that described
The material of mirror is fused quartz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721533494.5U CN207571378U (en) | 2017-11-16 | 2017-11-16 | A kind of liquid submerges high-NA microcobjective admittedly |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721533494.5U CN207571378U (en) | 2017-11-16 | 2017-11-16 | A kind of liquid submerges high-NA microcobjective admittedly |
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| CN207571378U true CN207571378U (en) | 2018-07-03 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799603A (en) * | 2017-11-16 | 2019-05-24 | 长光华大基因测序设备（长春）有限公司 | A kind of liquid submerges high-NA microcobjective and sequencing approach admittedly |
| CN113039603A (en) * | 2018-11-30 | 2021-06-25 | 松下知识产权经营株式会社 | Objective lens, optical head device, optical information device, and optical disc system |
-
2017
- 2017-11-16 CN CN201721533494.5U patent/CN207571378U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799603A (en) * | 2017-11-16 | 2019-05-24 | 长光华大基因测序设备（长春）有限公司 | A kind of liquid submerges high-NA microcobjective and sequencing approach admittedly |
| CN113039603A (en) * | 2018-11-30 | 2021-06-25 | 松下知识产权经营株式会社 | Objective lens, optical head device, optical information device, and optical disc system |
| CN113039603B (en) * | 2018-11-30 | 2023-04-07 | 松下知识产权经营株式会社 | Objective lens, optical head device, optical information device, and optical disc system |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 130033 No. 1 Building, Incubation Base, 77 Yingkou Road, Changchun Economic Development Zone, Jilin Province Patentee after: Changchun Changguang Huada Zhizao sequencing Equipment Co.,Ltd. Address before: 130033 Building 1, hatchery, 77 Yingkou Road, Changchun Economic Zone, Jilin Patentee before: CHANGGUANG HUADA GENE SEQUENCING EQUIPMENT (CHANGCHUN) Co.,Ltd. |
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