CN207528624U - A kind of solution surface reflects absorbance measurement device - Google Patents
A kind of solution surface reflects absorbance measurement device Download PDFInfo
- Publication number
- CN207528624U CN207528624U CN201721597052.7U CN201721597052U CN207528624U CN 207528624 U CN207528624 U CN 207528624U CN 201721597052 U CN201721597052 U CN 201721597052U CN 207528624 U CN207528624 U CN 207528624U
- Authority
- CN
- China
- Prior art keywords
- measurement device
- solution
- plane mirror
- light
- absorbance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a kind of solution surfaces to reflect absorbance measurement device, the device is the characteristic that propagation path of light can be changed based on optical fiber, design matches the solution surface reflection absorbance measurement device used with VIS 723N type visible spectrophotometers, including components such as pedestal, optical fiber, plane mirror and cuvettes, purpose is to change the incident light propagation direction of instrument, so that the incident light of light source for instrument is reflected in solution surface, it is received by detector end of the light beam that solution reflects in instrument, so as to measure the reflection absorbance of solution.By test, which has the following performance：Measurement device base dimensions are matched with visible spectrophotometer, can facilitate fixed and dismounting；The light that light source for instrument is sent out can receive after device reflects in detector end；During replication, absorbance change existsChange in the range of 0.010.The assay device structures are simple, at low cost, highly practical.
Description
Technical field
The utility model is related to reflect absorbance measurement device, specifically a kind of solution surface for changing light path based on optical fiber
Reflect absorbance measurement device.
Background technology
Absorption photometry is by the absorbance of spectrophotometric determination solution, and substance is determined according to one law of Beer of lambert
The method of solution concentration.The advantages that solution absorption photometry is because of high sensitivity, instrument and equipment is simple and convenient to operate, quick, is ring
Border monitors one of most common instrument analytical method.Nowadays the development trend of environmental monitoring is online, real-time, automatic.Using suction
When light photometry detects sample, the influence of refraction, scattering in order to avoid particulate matter generation in environmental water sample need to filter out water in advance
Particulate matter in sample obtains clear solution.Therefore, it to realize from automatically analyzing for measure overall process is sampled, water sample is realized
On-line filtration is particularly important.However, for field monitoring instrument, during long-term lay, due to water turbidity, online
Filter device filter membrane is vulnerable to the blocking of particulate matter, and then online auto monitoring system can be caused to paralyse, using absorption photometry as
The online monitoring instruments operating time at the scene on basis is limited to the service life of filter device, thus on-line filtration already into
Hinder the bottleneck of online auto monitoring.As can exploitation one kind saves on-line filtration step, water in only drainage sample is directly measured
The absorption photometric method of matter index, by the great service life for extending online monitoring instruments.
Invention content
Solid-phase reflection spectrophotometry is the analysis method that the surface reflection-absorption based on coloring matter is established, and can directly be surveyed
The fixed opaque surface of solids is to the degree of absorption of light.It is inspired by this, the utility model design can be with visible spectrophotometer
The solution surface reflection absorbance measurement device matched, and applied to the measure of only drainage sample Nitrite ion, demonstration is built
The absorption photometric method feasibility of water quality index in vertical only drainage sample.So as to which the online auto monitoring for only drainage sample carries
For theories integration.
A kind of solution surface reflection absorbance measurement device of the utility model, is that propagation path of light can be changed based on optical fiber
Characteristic, design match the solution surface reflection absorbance measurement device used, mesh with VIS-723N type visible spectrophotometers
Be change instrument incident light propagation direction so that the incident light of light source for instrument is reflected in solution surface, by solution
The light beam reflected is received in the detector end of instrument, so as to measure the reflection absorbance of solution.
A kind of solution surface reflection absorbance measurement device of the utility model, including pedestal, optical fiber, plane mirror and colorimetric
Ware；
The size of pedestal matches with visible spectrophotometer；
It is spaced apart on pedestal, first fixed block and second at fixed optical fiber both ends is respectively equipped with perpendicular to pedestal
Fixed block；
It is horizontal on the first fixed block to open up entering light through-hole for connecting the incident light input end of optical fiber；
Second fixed block includes integrally formed convex block, and light extraction through-hole is vertically opened up on convex block for connecting the incidence of optical fiber
Light exit end；
Plane mirror is set in the lower section of light extraction through-hole, plane mirror is set horizontal by 45 degree of angles；
Cuvette is tightly attached on the minute surface of plane mirror.
On pedestal, perpendicular to the locating slot of the base of the second fixed block setting plane mirror, and in the convex of the second fixed block
One overturning angle of block opens up the card slot horizontal by 45 degree of angles and puts plane mirror for blocking.
The pedestal is equipped with mounting hole, convenient to fix and dismantle with visible spectrophotometer.
The plane mirror is reflected into instrument by the light beam that solution reflects horizontal by 45 degree of angles through plane mirror
The end of detector receives, so as to measure the reflection absorbance of solution.
Absorbance measurement device is reflected using the utility model solution surface, establishes only drainage sample Nitrite ion
Reflection absorption photometric method, total thinking of method is, nitrite ion color developing agent is added in directly in only drainage sample, instead
Ying Hou measures the reflection absorbance of developing solution using device；Meanwhile the relationship of reflection absorbance and turbidity is investigated by testing,
Analyze the affecting laws of the turbidity pair only reflection absorbance measurement of drainage sample developing solution, obtain water sample reflection absorbance correct it is public
Formula；Using correction formula, to the reflection absorbance of developing solution into amendment, so as to accurate quantitative analysis only drainage sample Nitrite from
Sub- concentration.
Reflection absorbance measurement device is mounted on VIS-723N type vis spectroscopy light by the reflection absorbance measurement method
It spends on the colorimetric pool frame pedestal of meter, installs plane mirror, by sliding back and forth reflection absorbance measurement device micromatic setting position,
When detector receives largest light intensity, you can fixation reflex absorbance measurement device, real-time using instrument measure function, with
Ultra-pure water is reference, and the solution absorbance measured is to reflect absorbanceA。
It is described obtain water sample reflection absorbance correction formula method be：Prepare different turbidity（T）The serial nitrous of substrate
Acid ion standard solution adds in chromogenic reagent solution with reference to nitrite ion standard determination method, reacts 20 min, in use
Reflection absorbance measurement method is stated, measures the reflection absorbance of each solution；Analysis reflection absorbance and nitrite ion concentration
With the relationship of turbidity, using regression analysis, the correction formula that turbidity influences reflection absorbance is obtainedA T =f（T）。
The assay method of the only drainage sample Nitrite ion is substrate compound concentration as 0-0.64 using ultra-pure water
Serial 50.00 mL of nitrite ion standard solution in the range of mg/L is added in reference to nitrite ion standard determination method
Chromophoric solution reacts 20 min, by reflection absorbance measurement method, under 540 nm wavelength, measures the reflection of each standard solution
AbsorbanceA, with nitrite ion concentration（C P ）For abscissa, absorbance is reflectedAFor ordinate, standard curve is drawn；It takes not
50.00 mL of water sample is filtered, same amount of colour reagent and reaction are added in as standard curve by standard curve determination method
After same time, absorbance is measuredA S ；Meanwhile according to the standard determination method of turbidity]Measure the turbidity of water sampleT, by A T =f（T）
It calculatesA T ,Then, willA S -A T Only drainage sample Nitrite ion concentration can be calculated by substituting into standard curve regression equation.
By test, the utility model solution surface reflection absorbance measurement device has the following performance：
1st, measurement device base dimensions are matched with visible spectrophotometer, can facilitate fixed and dismounting；
2nd, the light that light source for instrument is sent out can receive after device reflects in detector end；
3rd, during replication, absorbance change existsChange in the range of 0.010.
The assay device structures are simple, at low cost, highly practical.
Description of the drawings
Fig. 1 is the structure diagram that embodiment solution surface reflects absorbance measurement device；
Fig. 2 is the 3D figures that embodiment solution surface reflects absorbance measurement device；
Fig. 3 is the index path that embodiment solution surface reflects absorbance measurement device.
In figure, 1. pedestal, 2. optical fiber, 3. plane mirror, 4. cuvette, 5. first fixed block 5-1. entering light through-holes
6. the second fixed block 6-1. convex block 6-1-1. light extraction through-hole 6-1-2. card slot 6-2. bases 7. are fixed
Position 8. location hole of slot.
Fig. 4 is Application Example device to test turbidity to reflecting absorbance influence curve.
Fig. 5 is the measurement result that actual water sample Nitrite ion is measured using classical way and the utility model method
Relationship.
Specific embodiment
The utility model content is further described with reference to the accompanying drawings and examples, but is not to the utility model
Restriction.
With reference to Fig. 1, a kind of solution surface reflects absorbance measurement device, according to test request, is drawn out in AutoCAD
Measurement device 3D schematic diagrames, as shown in Figure 2.The measurement device, including pedestal 1, optical fiber 2, plane mirror 3 and cuvette 4；In pedestal
It is spaced apart on 1, first fixed block 5 and the second fixed block 6 at fixed 2 both ends of optical fiber are respectively equipped with perpendicular to pedestal 1；
It is horizontal on the first fixed block 5 to open up entering light through-hole 5-1 for connecting the incident light input end of optical fiber 2；
Second fixed block 6 includes integrally formed convex block 6-1, and light extraction through-hole 6-1-1 is vertically opened up on convex block 6-1 for connecting
Connect the incident light port of export of optical fiber 2；
Plane mirror 3 is set in the lower section of light extraction through-hole 6-1-1, plane mirror 3 is set horizontal by 45 degree of angles；
Cuvette 4 is tightly attached on the minute surface of plane mirror 3.
On pedestal 1, perpendicular to the locating slot 7 of the base 6-2 setting plane mirrors 3 of the second fixed block 6, and fixed second
The bottom right overturning angle of the convex block 6-1 of block 6 opens up the card slot 6-1-2 horizontal by 45 degree of angles and puts plane mirror 3 for blocking.
Pedestal 1 is equipped with location hole 8, convenient to fix and dismantle with visible spectrophotometer.
With reference to the index path of Fig. 3 solution surfaces reflection absorbance measurement device, the incident light of light source for instrument passes through in device
The entering light through-hole of first fixed block enters optical fiber, is reflected in solution surface, and the light beam reflected by solution is in instrument
Detector end receive, so as to measure the reflection absorbance of solution.
The application result of the present embodiment surface reflection absorbance measurement device is as follows：
1st, reflection absorbance and turbidity and the relationship of nitrite ion concentration
Absorbance measurement device is reflected using solution surface, according to the only drainage sample Nitrite ion of above-mentioned foundation
Absorption photometric method is reflected, determines the serial nitrite ion standard solution of 0 ~ 0.64 mg/L in different turbidity substrates（0~
200 NTU）Under reflection absorbance, under identical turbidity substrate, absorbance is directly proportional to nitrite ion concentration, they
As listed in table 1, the slope of each regression equation is 1.360 ± 0.095 to equation of linear regression（n=12）, slope keeps one substantially
It causes, illustrates, turbidity does not interfere with the sensitivity of method, i.e., often increases the absorbance increasing that the nitrite ion of unit concentration generates
Value is not influenced by turbidity.As it can be seen that because the absorbance that nitrite ion generates has with the absorbance generated by turbidity substrate
Additive property.As can seek absorbance and the relational expression of turbidityA T =f（T）, then measure only drainage sample Nitrite from
During sub- concentration, only total absorbance need to be measured according to standard method（A S ）And turbidity（T）, according to formulaA T =f（T）It calculatesA T ,It willA S -A T Only drainage sample Nitrite ion concentration can be calculated by substituting into standard curve regression equation.
Under the different turbidity substrates of table 1, absorbance and concentration relationship are reflected
。
2nd, turbidity is derived and is verified on the correction formula that reflection absorbance influences
Each regression equation intercept in table 1, theoretically nitrite ion it is a concentration of 0 when, the absorbance of solution to be measured, by
It is found that under different turbidity, each regression equation intercept differs table 1, this is mainly because of the suction of the turbidity generation in solution to be measured
LuminosityA T .Therefore, withA T For ordinate, turbidityTFor abscissa, relation curve is drawn, as shown in figure 4, as turbidity is to reflecting extinction
Spend influence curve.Fig. 5 showsA T With turbidityTInto notable linear correlation（P= 6.50×10-4）, linear relationship Regression Equations
ForA T = 7×10-4 T+ 0.011（N=12, R2= 0.9617）, regression equation is the amendment that turbidity influences reflection absorbance
Formula.
In order to further verify the universality of correction formula and reliability.10 various concentration difference turbidity are prepared at random
The nitrite ion standard solution of substrate by the assay method of only drainage sample Nitrite ion, makes standard curve,
Each solution Nitrite ion concentration is measured, the results are shown in Table 2.Using compound concentration as the error of true data calculation measured value（）, the results are shown in Table 2.Table 2 statistics indicate that, the error of the measured value concentration of solution -
Between 5.83% ~ 3.33%, the absorbance correction formula for illustrating to derive is reliable, has certain universality.
2 random concentration of table, the measurement result of random turbidity water sample
。
3rd, the measurement result of actual water sample Nitrite ion
On June 24th, 2017, in Guilin Electronic Science and Technology Univ. Hua Jiang school district landscape lakes and the flower river acquisition of Guilin City spirit Tian Town
10 water samples.Each water sample is divided into two parts, a filtered, and measuring nitrite ion therein using standard method contains
Amount, another with the utility model proposes method measure.Using classical way measurement result as abscissa,XIt represents, this method is surveyed
Result is determined for ordinate,YIt represents, the results are shown in Figure 5, and Fig. 5 shows that significant linear relationship, linear regression is presented in the two
Equation isY = 1.0087X- 0.0005（N=10, R2= 0.9926）, regression equation slope illustrates that the two is measured close to 1
As a result there was no significant difference, illustrate the utility model proposes method can be applied to the survey of actual water sample Nitrite ion
It is fixed.
The utility model changes the solution surface reflection absorbance measurement device of light path and commercial spectrophotometric based on optical fiber
Meter matches, and has studied influence of the turbidity to nitrite ion reflection absorbance using the device, finds sample because of turbidity
The reflection absorbance of generation and the reflection absorbance of nitrite ion have additive property, pass through the recurrence to lot of experimental data
Analysis obtains the correction formula that turbidity influences reflection absorbance.And method is applied to the measure of only drainage sample, with mark
Quasi- method measurement result is without significant difference.The bottleneck that being developed into online test method, on-line filtration can be avoided problem.
Claims (3)
1. a kind of solution surface reflects absorbance measurement device, including pedestal（1）, optical fiber（2）, plane mirror（3）And cuvette
（4）；It is characterized in that：
In pedestal（1）It is upper spaced apart, perpendicular to pedestal（1）It is respectively equipped with fixed optical fiber（2）First fixed block at both ends
（5）With the second fixed block（6）；
In the first fixed block（5）Upper level opens up entering light through-hole（5-1）For connecting optical fiber（2）Incident light input end；
Second fixed block（6）Including integrally formed convex block（6-1）, convex block（6-1）On vertically open up light extraction through-hole（6-1-1）With
In connection optical fiber（2）The incident light port of export；
In light extraction through-hole（6-1-1）Lower section setting plane mirror（3）, plane mirror（3）It is set horizontal by 45 degree of angles；
Cuvette（4）It is tightly attached to plane mirror（3）Minute surface on.
2. solution surface according to claim 1 reflects absorbance measurement device, it is characterised in that：In pedestal（1）On, it hangs down
Directly in the second fixed block（6）Base（6-2）Plane mirror is set（3）Locating slot（7）, and in the second fixed block（6）Convex block
（6-1）An overturning angle open up card slot horizontal by 45 degree of angles（6-1-2）Plane mirror is put for card（3）.
3. solution surface according to claim 1 reflects absorbance measurement device, it is characterised in that：The pedestal（1）On
Equipped with location hole（8）, it is convenient to fix and dismantle with visible spectrophotometer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721597052.7U CN207528624U (en) | 2017-11-26 | 2017-11-26 | A kind of solution surface reflects absorbance measurement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721597052.7U CN207528624U (en) | 2017-11-26 | 2017-11-26 | A kind of solution surface reflects absorbance measurement device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207528624U true CN207528624U (en) | 2018-06-22 |
Family
ID=62580735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721597052.7U Expired - Fee Related CN207528624U (en) | 2017-11-26 | 2017-11-26 | A kind of solution surface reflects absorbance measurement device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207528624U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107677622A (en) * | 2017-11-26 | 2018-02-09 | 桂林电子科技大学 | A kind of solution surface reflects absorbance measurement device |
| CN107941719A (en) * | 2017-11-26 | 2018-04-20 | 桂林电子科技大学 | A kind of solution surface reflection absorbance measurement device and its application |
-
2017
- 2017-11-26 CN CN201721597052.7U patent/CN207528624U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107677622A (en) * | 2017-11-26 | 2018-02-09 | 桂林电子科技大学 | A kind of solution surface reflects absorbance measurement device |
| CN107941719A (en) * | 2017-11-26 | 2018-04-20 | 桂林电子科技大学 | A kind of solution surface reflection absorbance measurement device and its application |
| CN107941719B (en) * | 2017-11-26 | 2023-08-18 | 桂林电子科技大学 | Solution surface reflection absorbance measuring device and application thereof |
| CN107677622B (en) * | 2017-11-26 | 2023-09-26 | 桂林电子科技大学 | Solution surface reflection absorbance measuring device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI375025B (en) | System and method for measuring analyte concentration of a chemical or biological substance | |
| CN108020504B (en) | Optical measuring instrument based on quantum weak measurement and method for measuring and analyzing refractive index, optical rotation spectrum and chiral molecule enantiomer content of sample | |
| CN109799203B (en) | Wide-range high-precision spectrum detection method for COD concentration in water body | |
| WO2012075958A1 (en) | Real-time online absorption detection system | |
| WO2021179592A1 (en) | Liquid absorption coefficient measurement device and measurement method | |
| CN103217403B (en) | For measuring the device of transmissivity | |
| CN102012413A (en) | Device for chromatographic quantitative measurement | |
| CN103499393B (en) | The measuring method of spectrum | |
| CN207528624U (en) | A kind of solution surface reflects absorbance measurement device | |
| CN103499391A (en) | Spectrum measuring system | |
| CN109724780A (en) | A kind of small light spot test macro and its test method | |
| CA2081680C (en) | Spectrometer for conducting turbidimetric and colorimetric measurements | |
| CN104964940A (en) | Detection device and method for rapidly detecting content of total phosphorus in water sample | |
| CN105158184A (en) | Gas online analysis device based on optical integrating sphere | |
| CN107941719A (en) | A kind of solution surface reflection absorbance measurement device and its application | |
| CN107677622A (en) | A kind of solution surface reflects absorbance measurement device | |
| JPS5892844A (en) | Method and device for colorimetric measurement | |
| CN204789319U (en) | Detection apparatus for total phosphorus content in short -term test water sample | |
| CN213021948U (en) | Photometric measurement device applied to distribution photometer | |
| CN211602875U (en) | Chemical oxygen demand tester calibrating installation | |
| CN111257259A (en) | Water quality detection method and equipment | |
| CN219935666U (en) | Photoelectric detector for liquid concentration | |
| CN201903498U (en) | Integrating sphere for dry type chemical detection | |
| SU1511645A1 (en) | Multiple-pass dish for photometric analysis of samples of gas and liquid | |
| CN206161529U (en) | Portable combined detection analysis appearance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180622 Termination date: 20181126 |