CN207498398U - Tunable cell culture dish scoring devices - Google Patents
Tunable cell culture dish scoring devices Download PDFInfo
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- CN207498398U CN207498398U CN201721579826.3U CN201721579826U CN207498398U CN 207498398 U CN207498398 U CN 207498398U CN 201721579826 U CN201721579826 U CN 201721579826U CN 207498398 U CN207498398 U CN 207498398U
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- fixator
- gear
- lifting platform
- cell
- culture dish
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Abstract
The utility model discloses a kind of tunable cell culture dish scoring devices, are made of table mechanism, operating mechanism and control mechanism, and operating mechanism and control mechanism are separately positioned on table mechanism；In use, culture dish is placed on working plate, operating mechanism can be moved up and down in elevating lever, can cell scratch depth be observed by display screen, it is mutually engaged with gear grooved by gear, can adjust the back-and-forth motion of screeding device, can cell cut length be observed by display screen；The utility model is scientific and reasonable for structure, it is easy to operate, width and the cut of depth point-device can be fixed, scratch depth can adjust, and not influence the bed board material of cell lower part, cut registration, scratch width can carry out drawing plate according to different cut tools, can observe the ability level of cell migration, have video camera auxiliary system, cell cut quality can be improved into line width, depth intense adjustment.
Description
Technical field
The utility model is related to a kind of medical instrument, more particularly to a kind of tunable cell culture dish scoring devices.
Background technology
Regular noun of the cell culture in biology is cell culture technology, no matter for entire biotechnology,
Or for the biological cloning technology of one of them, cell culture is all an essential process, and cell culture is inherently
It is the extensive clone of cell.Cell culture technology can become simple unicellular or pole by a cell by mass propgation
The many cells broken up less, this is the essential link of clone technology, and the clone of cell culture inherently cell, is passed through
Cell culture obtains a large amount of cell or its metabolite, because biological product is got from cell, it is possible to say cell
Culture technique is most crucial, most basic technology in biotechnology.
Cell scratch experiment refers to by cell culture on culture dish or tablet, is drawn after cell fusion in middle section
One line, the cell in this line are got rid of by mechanical force, then continue to cultivate by cell, and observation cell is drawn to acellular
The situation of trace zone migration, to judge the transfer ability of cell.
Existing cell cut is mostly manual cut, and manual cut, using sample-adding pipette tips cut, exists usually on culture dish
Following shortcoming：
Occurs rigid cut on culture dish：The purpose of cut is usually used in assessing the ability level of cell migration, manual cut
The gimmick of several weeks is often needed to practice cut, manual cut occurring rigid on vessel tablet on the cell plates for needing to detect
It is also common to scratch, and influences the observation of cell migration, and manual scratch width cannot be grasped, cannot using sample-adding pipette tips scratch width
It determines, randomness is strong, and manual scratch depth cannot control, and scoring operation person is often because cannot smooth culture dish bottom residual at cut
Cell, manual two pieces of culture dish cut comparisons of cut are inconsistent, often compare not objective enough, manual cut because scratch width is inconsistent
Width cannot be accurate to scale, position inaccurate, multiple observation same position mobilance again after being positioned after cut, after manual cut
The position of positioning cannot be accurate to, often cut is straight after manual cut, irregularity, and observing effect is bad, and manual cut cannot be again
Fixed thickness is retained on culture dish, born of the same parents' culture is multilayer in bottom tiling culture materials or gel or cell, and manual cut cannot
Accomplish to retain fixed thickness base material.
Invention content
The purpose of this utility model is that cell cut is the manual cut institute problem in the above-mentioned background technology of solution, and
A kind of tunable cell culture dish scoring devices are provided.
Tunable cell culture dish scoring devices, are made of table mechanism, operating mechanism and control mechanism, operate machine
Structure and control mechanism are separately positioned on table mechanism；
Table mechanism includes working plate, the first fixator, the second fixator, third fixator, elevating lever and culture
Ware, the first fixator, the second fixator, third fixator, elevating lever and culture dish are separately positioned on working plate, elevating lever
Working plate rear end is fixed at, before the first fixator, the second fixator and third fixator are separately positioned on working plate
End, culture dish is arranged between the first fixator, the second fixator and third fixator, the first fixator, the second fixator and
Third fixer structure is identical；
First fixator includes the first fixed block, fixing bolt and the second fixed block, the first fixed block and the second fixed block
Structure is identical, and is respectively fixedly disposed on working plate, and the first fixed block and the second fixed block upper end are respectively provided in first
Threaded hole, fixing bolt are each passed through the first internal thread hole of the first fixed block and the second fixed block, in fixing bolt and first
Corresponding threaded holes close；
There is the first graduation mark on elevating lever；
Operating mechanism includes fixed lifting gear, screeding device and driving device, and fixed lifting gear is arranged on elevating lever
On, screeding device and driving device are separately positioned on fixed lifting gear,
Fixed lifting gear includes fixes sleeve, the first fixation lifting platform, the second fixation lifting platform and the first leading screw shaft,
First fixation lifting platform and the second fixation lifting platform are separately positioned on fixes sleeve rear end, and first, which fixes lifting platform and second, fixes
The second coaxial internal thread hole is respectively provided on lifting platform, the first leading screw shaft upper end has first-hand, and lower end has first
Leading screw, the first leading screw shaft pass through first to fix lifting platform and second and fix in the second internal thread hole of lifting platform, the first leading screw
It is matched with the second internal thread hole；
There is clamping screw in fixes sleeve；
The structure on two sides of first fixation lifting platform is identical, and front end has the first sliding groove, and upper end has second sliding slot, and second slides
Slot both sides have the second graduation mark；
The structure on two sides of second fixation lifting platform is identical, and front end has third sliding slot, has gear grooved on third sliding slot,
Screeding device includes the first slide bar, the second slide bar, connecting rod and draws head, and the first slide bar and the second slide bar are fixed respectively
Connecting rod upper/lower terminal is arranged on, the first slide bar both ends are respectively provided with pointer head, and connecting rod lower end has jointing, draws head
Jointing lower end is arranged on, the first slide bar is arranged in the first sliding groove, and the second slide bar is arranged in third sliding slot, and pointer head is set
It puts in second sliding slot；
Driving device includes the second shaft and driver, and the second shaft is arranged in driver, has the in the second shaft
Second chief and axostylus axostyle, driver is interior to have gear, and gear is arranged on axostylus axostyle, and gear is mutually engaged with gear grooved；
Control mechanism includes the first image pick-up device, the second image pick-up device and display screen, and the first image pick-up device, the second image pick-up device are set respectively
It puts on working plate, the first image pick-up device and the second image pick-up device are connected respectively with display screen.
The operation principle and process of the utility model：
In use, culture dish is placed between the first fixator, the second fixator and third fixator, rotary fixing screw
Bolt, fixing bolt are matched with the first internal thread hole, and the first fixator, the second fixator and third fixator match fixed training
Ware is supported, the right position of culture dish can be adjusted, first-hand of the first leading screw shaft is rotated, by the first leading screw and second
Corresponding threaded holes close, and operating mechanism can be moved up and down in elevating lever, and the first image pick-up device and the second image pick-up device can accurate observation stroke heads
With the position relationship of cell, can cell scratch depth be observed by display screen, to according to necessary requirement, pass through the first of elevating lever
Graduation mark matches, and is adjusted the height for drawing head, rotates the second in command of the second shaft, is mutually stung with gear grooved by gear
Close, can adjust the back-and-forth motion of screeding device, by the second graduation mark, observable draw head movement front and rear in culture dish away from
From, can by display screen observe cell cut length, clamping screw can fixed operation mechanism fixation lifting gear.
The beneficial effects of the utility model：
The utility model is scientific and reasonable for structure, easy to operate, does not need to be further processed, and point-device can be consolidated
The cut of fixed width degree and depth, scratch depth can adjust, and not influence the bed board material of cell lower part, and cut registration is drawn
Trace width can carry out drawing plate according to different cut tools, can observe the ability level of cell migration, auxiliary with video camera
Auxiliary system can improve cell cut quality into line width, depth intense adjustment.
Description of the drawings
Fig. 1 is the partial structural diagram of the utility model.
Fig. 2 is the structure diagram of the utility model.
Fig. 3 is the structure diagram of the utility model.
Fig. 4 is the partial structural diagram of the utility model.
Fig. 5 is the structure diagram of the utility model cut mechanism.
Fig. 6 is the structure diagram that the utility model draws head first embodiment.
Fig. 7 is the structure diagram that the utility model draws head second embodiment.
Fig. 8 is the structure diagram of the utility model culture dish.
Specific embodiment
It please refers to Fig.1, shown in Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7 and Fig. 8, tunable cell culture dish scoring devices,
It is made of table mechanism 1, operating mechanism 2 and control mechanism 3, operating mechanism 2 and control mechanism 3 are separately positioned on workbench
In mechanism 1；
Table mechanism 1 includes working plate 11, the first fixator 12, the second fixator 13, third fixator 14, lifting
Bar 15 and culture dish 17, the first fixator 12, the second fixator 13, third fixator 14, elevating lever 15 and culture dish 17 are distinguished
It is arranged on working plate 11, elevating lever 15 is fixed at 11 rear end of working plate, the first fixator 12, the second fixator 13
11 front end of working plate is separately positioned on third fixator 14, culture dish 17 is arranged on the first fixator 12, the second fixator
Between 13 and third fixator 14, the first fixator 12, the second fixator 13 are identical with 14 structure of third fixator；
First fixator 12 includes the first fixed block 121,122 and second fixed block 123 of fixing bolt, the first fixed block
121 is identical with 123 structure of the second fixed block, and is respectively fixedly disposed on working plate 11, and the first fixed block 121 and second is solid
Determine 123 upper end of block and be respectively provided with the first internal thread hole, fixing bolt 122 is each passed through the first fixed block 121 and the second fixed block
123 the first internal thread hole, fixing bolt 122 are matched with the first internal thread hole；
There is the first graduation mark 151 on elevating lever 15；
Operating mechanism 2 includes fixed lifting gear 21, screeding device 22 and driving device 23, and fixed lifting gear 21 is set
On elevating lever 15, screeding device 22 and driving device 23 are separately positioned on fixed lifting gear 21,
Fixed lifting gear 21, which includes fixes sleeve 211, first, to be fixed lifting platform 212, second and fixes lifting platform 213 and the
One leading screw shaft 16, the first fixation lifting platform 212 and second fix lifting platform 213 and are separately positioned on 211 rear end of fixes sleeve, the
One fixation lifting platform 212 and second, which is fixed on lifting platform 213, is respectively provided with the second coaxial internal thread hole, the first leading screw shaft 16
Upper end is with first-hand 161, and lower end has the first leading screw 162, and the first leading screw shaft 16 passes through first to fix 212 He of lifting platform
Second fixes in the second internal thread hole of lifting platform 213, and the first leading screw 162 is matched with the second internal thread hole；
There is clamping screw 2111 in fixes sleeve 211；
The structure on two sides of first fixation lifting platform 212 is identical, and front end has the first sliding groove 2121, and upper end has second sliding slot
2122,2122 both sides of second sliding slot have the second graduation mark 2123；
The structure on two sides of second fixation lifting platform 213 is identical, and front end has third sliding slot 2131, has on third sliding slot 2131
There is gear grooved,
Screeding device 22 includes the first slide bar 221, the second slide bar 222, connecting rod 223 and draws head 225, the first slide bar 221
223 upper/lower terminal of connecting rod is respectively fixedly disposed at the second slide bar 222,221 both ends of the first slide bar are respectively provided with pointer head
226,223 lower end of connecting rod has jointing 224, draws head 225 and is arranged on 224 lower end of jointing, the first slide bar 221 is set
In the first sliding groove 2121, the second slide bar 222 is arranged in third sliding slot 2131, and pointer head 226 is arranged on second sliding slot 2122
On；
Driving device 23 includes the second shaft 231 and driver 232, and the second shaft 231 is arranged in driver 232, the
In two shafts 231 there is the second in command 233 and axostylus axostyle, there is in driver 232 gear, gear is arranged on axostylus axostyle, gear with
Gear grooved is mutually engaged；
Control mechanism 3 includes the first image pick-up device 31, the second image pick-up device 32 and display screen 33, and the first image pick-up device 31, second are taken the photograph
As device 32 is separately positioned on working plate 11, the first image pick-up device 31 and the second image pick-up device 32 are connected respectively with display screen 33.
The operation principle and process of the utility model：
It please refers to Fig.1, shown in Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7 and Fig. 8, in use, culture dish 17 is placed on first
Between fixator 12, the second fixator 13 and third fixator 14, rotary fixing screw bolt 122, spiral shell in fixing bolt 122 and first
Pit matches, and the first fixator 12, the second fixator 13 and third fixator 14 match fixed culture dish 17, can adjust
The right position of culture dish 17 rotates the first-hand 161 of the first leading screw shaft 16, passes through spiral shell in the first leading screw 162 and second
Pit matches, and operating mechanism 2 can be moved up and down in elevating lever 15, and the first image pick-up device 31 and the second image pick-up device 32 can accurately be seen
The position relationship for drawing head 225 and cell is examined, can cell scratch depth be observed by display screen, to according to necessary requirement, pass through liter
First graduation mark 151 of drop bar 15 matches, and is adjusted the height for drawing head 22, rotates the second in command of the second shaft 231
233, it is mutually engaged with gear grooved by gear, can adjust the back-and-forth motion of screeding device 22, it is considerable by the second graduation mark 2123
It examines and draws the displacement distance front and rear in culture dish 17 of head 225, can cell cut length, clamping screw 2111 be observed by display screen
Can fixed operation mechanism 2 fixation lifting gear 21.
Claims (1)
1. a kind of tunable cell culture dish scoring devices are by table mechanism (1), operating mechanism (2) and control mechanism (3)
Composition, operating mechanism (2) and control mechanism (3) are separately positioned on table mechanism (1)；
Table mechanism (1) includes working plate (11), the first fixator (12), the second fixator (13), third fixator
(14), elevating lever (15) and culture dish (17), the first fixator (12), the second fixator (13), third fixator (14), lifting
Bar (15) and culture dish (17) are separately positioned on working plate (11), after elevating lever (15) is fixed at working plate (11)
End, the first fixator (12), the second fixator (13) and third fixator (14) are separately positioned on working plate (11) front end, train
It supports ware (17) to be arranged between the first fixator (12), the second fixator (13) and third fixator (14), the first fixator
(12), the second fixator (13) is identical with third fixator (14) structure；
First fixator (12) includes the first fixed block (121), fixing bolt (122) and the second fixed block (123), and first fixes
Block (121) is identical with the second fixed block (123) structure, and is respectively fixedly disposed on working plate (11), the first fixed block
(121) and the second fixed block (123) upper end is respectively provided with the first internal thread hole, and fixing bolt (122) is each passed through the first fixation
First internal thread hole of block (121) and the second fixed block (123), fixing bolt (122) are matched with the first internal thread hole；
There is the first graduation mark (151) on elevating lever (15)；
Operating mechanism (2) includes fixed lifting gear (21), screeding device (22) and driving device (23), fixed lifting gear
(21) it is arranged on elevating lever (15), screeding device (22) and driving device (23) are separately positioned on fixed lifting gear (21)
On,
Fixed lifting gear (21) fixes lifting platform (212), the second fixation lifting platform (213) including fixes sleeve (211), first
With the first leading screw shaft (16), the first fixation lifting platform (212) and the second fixation lifting platform (213) are separately positioned on fixes sleeve
(211) the second coaxial internal thread is respectively provided on rear end, the first fixation lifting platform (212) and the second fixation lifting platform (213)
Hole, first leading screw shaft (16) upper end have first-hand handle (161), and lower end has the first leading screw (162), the first leading screw shaft
(16) it fixes lifting platform (212) and second across first to fix in the second internal thread hole of lifting platform (213), the first leading screw
(162) it is matched with the second internal thread hole；
There is clamping screw (2111) in fixes sleeve (211)；
The structure on two sides of first fixation lifting platform (212) is identical, and front end has the first sliding groove (2121), and upper end has second sliding slot
(2122), second sliding slot (2122) both sides have the second graduation mark (2123)；
Second fix lifting platform (213) structure on two sides it is identical, front end has third sliding slot (2131), on third sliding slot (2131)
With gear grooved,
Screeding device (22) is including the first slide bar (221), the second slide bar (222), connecting rod (223) and draws head (225), and first slides
Bar (221) and the second slide bar (222) are respectively fixedly disposed at connecting rod (223) upper/lower terminal, the first slide bar (221) both ends point
Not Ju You pointer head (226), connecting rod (223) lower end have jointing (224), draw a head (225) be arranged on jointing
(224) lower end, the first slide bar (221) are arranged in the first sliding groove (2121), and the second slide bar (222) is arranged on third sliding slot
(2131) in, pointer head (226) is arranged in second sliding slot (2122)；
Driving device (23) includes the second shaft (231) and driver (232), and the second shaft (231) is arranged on driver (232)
It is interior, in the second shaft (231) there is the second in command (233) and axostylus axostyle, there is gear in driver (232), gear is arranged on axis
On bar, gear is mutually engaged with gear grooved；
Control mechanism (3) includes the first image pick-up device (31), the second image pick-up device (32) and display screen (33), the first image pick-up device (31),
Second image pick-up device (32) is separately positioned on working plate (11), and the first image pick-up device (31) and the second image pick-up device (32) are respectively with showing
Display screen (33) is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721579826.3U CN207498398U (en) | 2017-11-23 | 2017-11-23 | Tunable cell culture dish scoring devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721579826.3U CN207498398U (en) | 2017-11-23 | 2017-11-23 | Tunable cell culture dish scoring devices |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207498398U true CN207498398U (en) | 2018-06-15 |
Family
ID=62505878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721579826.3U Expired - Fee Related CN207498398U (en) | 2017-11-23 | 2017-11-23 | Tunable cell culture dish scoring devices |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207498398U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699488A (en) * | 2017-11-23 | 2018-02-16 | 吉林大学 | Tunable cell culture dish scoring devices |
-
2017
- 2017-11-23 CN CN201721579826.3U patent/CN207498398U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699488A (en) * | 2017-11-23 | 2018-02-16 | 吉林大学 | Tunable cell culture dish scoring devices |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180615 Termination date: 20201123 |
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| CF01 | Termination of patent right due to non-payment of annual fee |