CN207452135U - Utilize the equipment of electroporation technology extraction Chinese medicine drug ingedient - Google Patents
Utilize the equipment of electroporation technology extraction Chinese medicine drug ingedient Download PDFInfo
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- CN207452135U CN207452135U CN201721292484.7U CN201721292484U CN207452135U CN 207452135 U CN207452135 U CN 207452135U CN 201721292484 U CN201721292484 U CN 201721292484U CN 207452135 U CN207452135 U CN 207452135U
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Abstract
The utility model discloses a kind of equipment using electroporation technology extraction Chinese medicine drug ingedient.The equipment includes head tank, water pump, pulse electric field process chamber, collecting tank, high-voltage pulse power source and computer, and wherein high-voltage pulse power source and water pump is controlled by computer and run.Chinese medicine extract enters pulse electric field process chamber from feed inlet, by between coaxial electrode, receiving high-pressure pulse electric, realizes the electroporation of cell membrane, then is discharged from discharge port, so as to obtain required effective component.This method in Chinese medicine extract, causes charge polarization effect using Electro-pulsing Field on Chinese medicine cell, generates electrostatic force, realizes the histiocytic electroporation of Chinese medicine, makes the membrane perforation of Chinese medicine cell, the drug ingedient dissolution inside cell membrane.The utility model can uniform, non-thermal, continuous work, realize to Chinese medicine batch extracting commercial Application.
Description
Technical field
The utility model belongs to traditional Chinese medicine ingredients extraction field, and in particular to a kind of to extract Chinese medicine medicine using electroporation technology
The equipment of object ingredient.
Background technology
With the development of the times, scientific and technical continuous progress, traditional Chinese medicine medicine materical crude slice also are occurring to change, Chinese medicine preparation
Particle is increasingly subject to the welcome and popularization of common people.Chinese medicinal granule to meet the prepared slices of Chinese crude drugs of concocted specification as raw material,
It extracts, concentrate through modern crafts, drying, the refined pure Chinese medicine product line formed of pelletizing.Convenient, peace that Chinese medicinal granule has
Entirely, effective advantage, for China's Chinese Traditional Medicine career development is promoted to be of great significance.
In process of production, active ingredient extraction is one of key technology of herbal pharmaceutical.The horizontal height of extractive technique is determined
The quality of Quality of Chinese Traditional Proprietary Medicine is determined.Chinese medicinal granule constituents extraction is mainly had generally using traditional handicraft at present：Decoction carries
Take, circulating reflux extraction, seepage pressure effects, adverse current filling group extraction etc., in heating unit close complete leach, acquisition extracting solution.
Traditional handicraft there are problems that being difficult to overcome：Recovery rate is low；Heating temperature is high, ingredient destructible；Extraction time is long；High energy consumption；
It is big to go out liquid coefficient, aggravates follow-up waste liquid environmental protection treatment burden；The active ingredient that extracts is complicated, active ingredient is indefinite and
Content ratio is more low.There are some new extractive techniques to apply to this field in succession in recent years, such as ultrasonic wave extraction, microwave
The methods of extraction, supercritical fluid extraction, molecular imprinting technology, semi-bionic extraction method, enzymolysis, normal-temperature superhigh pressure extraction.These are new
Technology show superiority, reduce cost, improve efficiency, but have respective limitation, develop new more effective
Method extraction traditional Chinese medicine resource it is extremely urgent.It has no and is set using the traditional Chinese medicine ingredients extraction of pulsed electric field methods currently on the market
It is standby.
Electroporation technology is different from traditional Chinese medicine and is formulated decocting drinking method, also different and microwave extract method, electroporation
Extractive technique refers between processed article is placed in two electrodes, and pulse high-voltage is imposed near room temperature（Representative value 2-80kV/
cm）Technology, action time is depending on recovery rate.The method is a kind of non-heat treated method.It is based on impulse electric field centering
The effect of medicinal powder end cell when Electro-pulsing Field causes charge polarization effect on herb powder cell medium, works as cell membrane
When upper voltage reaches critical value, concentration breakdown of membrane will be caused to destroy, so that vacuole ruptures in Chinese medicine tissue, in cell membrane
Portion effectively sets out spilling.
Current well accepted Electro-pulsing Field mechanism is theoretical for electroporation.Cytoplasm agglutination, wherein there are various
Ion（Such as Na+、K+、Ca2+）, it is considered as conductor.Cell membrane is phospholipid bilayer, has membrane capacitance and film resistance.When thin
Born of the same parents are placed in electric field, and due to electrostatic screening effect, applied voltage will be added on cell membrane, and transmembrane voltage is formed on cell membrane
（Transmembrane Potential, TMP）, the size of transmembrane voltage is related with the size of cell, radius extra electric field
（Swan equations）.With the increase of external electric field or the extension of processing time, transmembrane voltage constantly increases, the extruding that cell membrane is subject to
Power also increases, and thickness is gradually reduced.When transmembrane voltage reaches critical value（Representative value is 1V）When occur electroporation
（Electroporation）Phenomenon.When field strength is smaller, the perforation is reversible, can carry out cell transfecting（Transfection）；Field strength
When larger, perforation is irreversible, that is, permanent damage occurs, and active ingredient dissolves out rapidly at this time, can extract Related Component.During it is
Pharmaceutically active ingredient provides a kind of new research means, disclosure satisfy that low energy consumption, it is short to take, pollutes small, extraction efficiency
High demand for development.
The content of the invention
The purpose of this utility model is that propose a kind of equipment using drug ingedient in electroporation technology extraction Chinese medicine.It should
Device and method fill the extracting solution of Chinese medicine using impulse electric field bombardment, and charge polarization effect is caused on Chinese medicine cell, generate
Electrostatic force carries out electroporation to the cell of Chinese medicine, ruptures the vacuole in Chinese medicine cell tissue, the drug ingedient inside cell membrane
It overflows.Its outstanding advantage is, compared with problems, the impulse electric field side such as the time of traditional decoction method is long, energy consumption is big, efficiency is low
Method processing time is short（Several microsecond pulse magnitudes）, energy consumption it is low, also with extraction efficiency is high, heat production is few, uniformity is good, dirty
Contaminate the advantages that small, room temperature is extracted, is easy to operate.The method is a kind of very promising traditional Chinese medicine ingredients extracting method, in enhancing
The advantage of medicine and the modernization of traditional Chinese medicine are of great significance.
The utility model proposes it is a kind of using electroporation technology extraction Chinese medicine drug ingedient equipment, including raw material
Tank, water pump, pulse electric field process chamber, collecting tank, high-voltage pulse power source and computer；Wherein,
The pulse electric field process chamber is by high-field electrode array, low-field electrode array, upper cover, lower cover, feed inlet, discharging
Mouth is made of an electrically conducting material with insulation outer barrel composition, the upper cover and lower cover, is respectively overlay in above insulation outer barrel under
Face；The high-field electrode array includes several high-field electrodes, and for each high-field electrode to be thin rodlike, one end is both secured to upper cover
On, upper cover connects the high-pressure side of high-voltage pulsed source；The low-field electrode array includes several low-field electrodes, each low tension
Extremely hollow tubular is fixed into array structure by several metal dish, and low-field electrode is a pair of with coaxial configuration one with high-field electrode
Should, low-field electrode is enclosed in outside corresponding high-field electrode, and low-field electrode array one end is connected with lower cover, lower cover ground connection；It is described
Feed inlet be located on upper lid, the discharge port is located on lower lid；In use, Chinese medicine is between high-field electrode and low-field electrode
Field gap in be subject to electric field action；
Described water pump one end is connected with head tank, and the other end is connected with the feed inlet of pulse electric field process chamber；
The discharge port of the pulse electric field process chamber is connected with collecting tank；
The high-pressure side of the high-voltage pulse power source is connected with the upper cover of pulse electric field process chamber, at ground terminal and impulse electric field
Manage the lower cover of room altogether；
The computer keeps communicating to connect with water pump and high-voltage pulse power source respectively, controls water pump and high-voltage pulse electric
The working condition in source.
In the utility model, for the high-voltage pulse rising edge of high-voltage pulse power source output in below 300ns, pulse is wide
It spends for 1 μ s-100 μ s.
In the utility model, the spacing of field gap is 0.5- between the high-field electrode array and low-field electrode array
5cm, the pulse voltage of high-voltage pulse power source output is 1-100kV；The high-voltage pulse power source output voltage is in height
The electric field strength generated between piezoelectricity pole and low-field electrode is in 2-200kV/cm.
In the utility model, the high-field electrode array and low-field electrode array is uniformly distributed and dismantled and assembled, number of electrodes
Amount is according to the electrical parameter of high-voltage pulse power source and the treating capacity flexible modulation of drug.
Traditional Chinese medicine ingredients extraction equipment described in the utility model further includes flowmeter, and flowmeter provides flow number to computer
According to feedback signal, the feedback signal that the computer is provided by flowmeter controls the work of water pump and high-voltage pulse power source
Make state, the traditional Chinese medicine ingredients extraction equipment is made to be in continuous duty or service intermittent state.
The utility model further relates to a kind of method using the traditional Chinese medicine ingredients extraction equipment extraction Chinese medicine ingredient,
It uses high-voltage pulse electric field alignment medicine cell to carry out electroporation processing, and comprises the following steps：
（1）By Chinese medicine cut distribution into fritter, it is immersed in low conductivity extracting solution, forms suspension, and suspension is added in
Head tank；
（2）Water pump and high-voltage pulse power source are controlled by computer, connect high-voltage pulse power source, and opens water pump, water pump
Raw material suspension is pumped into the feed inlet of pulse electric field process chamber, by the field gap between high-field electrode and low-field electrode；
（3）Suspension is extracted drug ingedient, and is discharged into collecting tank from discharge port after electric field action；
（4）Product after electric field action obtains required drug ingedient after separating-purifying.
The utility model further relates to a kind of side using the traditional Chinese medicine ingredients extraction equipment extraction Chinese medicine drug ingedient
Method uses high-voltage pulse electric field alignment medicine cell to carry out electroporation processing, and comprises the following steps：
（1）By Chinese medicine cut distribution into fritter, it is placed in the field gap between high-field electrode and low-field electrode, and to head tank
Middle addition low conductivity extracting solution；
（2）Water pump and high-voltage pulse power source are controlled by computer, connect high-voltage pulse power source, and opens water pump, water pump
Liquid for Low Conductance is pumped into the feed inlet of pulse electric field process chamber, by the field gap between high-field electrode and low-field electrode
Interior Chinese medicine；
（3）The low conductivity extracting solution of Chinese medicine is impregnated after electric field action, is extracted drug ingedient, and from discharge port quilt
It is discharged into collecting tank；
（4）Product after electric field action obtains required drug ingedient after separating-purifying.
In the method for extraction traditional Chinese medicine ingredients described in the utility model, the Chinese medicine is graininess, powdered or thin
Sheet.
In the method for extraction traditional Chinese medicine ingredients described in the utility model, the low conductivity extracting solution is deionized water
Or alcohol.
The beneficial effects of the utility model are：
（1）The traditional Chinese medicine ingredients extraction equipment uses the structure of coaxial electrode, simple in structure, easy to process, handling；
In coaxial-type extraction structure, handle indoor electric field and the distribution of horizontal radiation shape is presented, it is vertical with extracting solution flow direction, ensure big portion absolutely
Cell suspension is divided all to be applied in sufficient number of electric pulse；In coaxial-type extraction structure, low-field electrode is wrapped in high-field electrode
Periphery, can keep the gnotobasis of closing, and prevent the hidden danger of high pressure electric leakage and electromagnetic radiation.
（2）The traditional Chinese medicine ingredients extraction equipment uses stream-handling pattern, and is expanded with dismountable electrod-array
Treatment scale adapts to the requirement of industrialized mass production；Can be when in use according to the demand of actual treatment amount, selection is suitably
Electrod-array quantity improves energy utilization efficiency.
（3）The traditional Chinese medicine ingredients extraction equipment by computer control can realize extracting solution continuous processing pattern or
Discontinuity tupe with reference to actual treatment amount and the requirement control process sequential of recovery rate, so as to reduce fuel factor, improves energy
Measure utilization ratio.
（4）The traditional Chinese medicine ingredients extracting method processing time is short, energy consumption are low, also with extraction efficiency is high, heat production
Less, the advantages that uniformity is good, pollution is small, room temperature is extracted, is easy to operate.The method is a kind of very promising traditional Chinese medicine ingredients extraction side
Method is of great significance for enhancing the modernization of advantage and traditional Chinese medicine of Chinese medicine.
Description of the drawings
Fig. 1 is the schematic diagram of impulse electric field extraction equipment.
Fig. 2 is low-field electrode array junctions composition.
Fig. 3 is high-field electrode array junctions composition.
Fig. 4 is half-sectional structure chart inside pulse electric field process chamber.
Fig. 5 is pulse electric field process chamber electrode array configurations figure.
Fig. 6 is pulse electric field process chamber outside drawing.
Figure label：1 is head tank, and 2 be water pump, and 3 be flowmeter, and 4 be pulse electric field process chamber, and 5 be collecting tank, and 6 are
Computer, 7 be high-voltage pulse power source, and 8 be metal dish, and 9 be low-field electrode, and 10 be metal dish, and 11 be high-field electrode, and 12 be non-gold
Belong to supporting item, 13 be discharge port, and 14 be upper cover, and 15 be insulation outer barrel, and 16 be lower cover, and 17 be feed inlet.
Specific embodiment
The utility model is further illustrated below by embodiment combination attached drawing.These embodiments are merely to illustrate this practicality
New rather than limitation the scope of the utility model.
Embodiment 1：
As shown in Figure 1, the equipment includes head tank 1, water pump 2, pulse electric field process chamber 4, collecting tank 5, high-voltage pulse
Power supply 7 and computer 6；Wherein,
The pulse electric field process chamber is by high-field electrode array, low-field electrode array, upper cover 14, lower cover 16, feed inlet
17th, discharge port 13 and insulation outer barrel 15 form, and Chinese medicine is subject to electricity in the field gap between high-field electrode 11 and low-field electrode 9
Field action；
The upper cover 14 and lower cover 16 is made of conductive material, overlays on above insulation outer barrel 15 and following；
Each high-field electrode 11 in the high-field electrode array is thin rodlike, is fixed in upper cover 14, upper cover 14 connects
Connect the high-pressure side of high-voltage pulse power source 7；
Each low-field electrode 9 in the low-field electrode array is hollow tubular, and battle array is fixed by several metal dish
Array structure, low-field electrode 9 are corresponded with high-field electrode 11 with coaxial configuration, are enclosed in outside corresponding high-field electrode, low tension
Pole array is connected with lower cover 16, and lower cover 16 is grounded；
The feed inlet 17 and discharge port 13, one is opened in upper cover 14, another is opened on lower cover 16；
Described 2 one end of water pump is connected with head tank 1, and the other end is connected with the feed inlet 17 of pulse electric field process chamber 4；
The discharge port 13 of the pulse electric field process chamber 4 is connected with collecting tank 5；
The high-pressure side of the high-voltage pulse power source 7 is connected with the upper cover 14 of pulse electric field process chamber 4, ground terminal and pulse electricity
The lower cover 16 of field process chamber 4 is altogether；
The computer 6 keeps communicating to connect with water pump 2 and high-voltage pulse power source 7 respectively, controls water pump 2 and high-tension pulse
Rush the working condition of power supply 7.
It is as follows：
（1）The low-field electrode array of traditional Chinese medicine ingredients extraction equipment shares 20, with upper and lower two panels stainless steel disk such as Fig. 2 institutes
Show that equably arrangement is fixed, and be connected on the lower lid of pulse electric field process chamber and be grounded；10 high-field electrodes are chosen, such as Fig. 3 institutes
Show that threadingly equably arrangement is fixed on the upper lid of electric field pulse electric field processing chamber, high-field electrode and low-field electrode it
Between field gap for 0.5cm, length 1m,, will not be right since low-field electrode is directly grounded after extra low-field electrode is blocked
Equipment generates any influence；
（2）By Radix Salviae Miltiorrhizae sample comminution into 70 mesh powders, and by the powder and alcohol with 1:3 ratio is sufficiently mixed, system
Into after raw material suspension, head tank is added in；
（3）High-voltage pulse power source is connected, pulse voltage 1kV, frequency 20Hz, rising edge 100ns, 1 μ s of pulsewidth, electric field are set
Generated electric field strength 2kV/cm in gap；
（4）Open water pump, raw material suspension be continuously pumped into the feed inlet of pulse electric field process chamber, from the bottom to top with
The flow velocity of 0.1m/s is by the field gap between high-field electrode and low-field electrode；
（5）The suspension of Danshen Root and alcohol after the bombardment of 200 sub-high pressure impulse electric fields, the tanshinone that is extracted and
Suspension is discharged into collecting tank from discharge port together；
（6）Product mixtures obtain required drug ingedient after later separation purifies, and find its tanshinone after testing
Leaching rate up to 85%.
Embodiment 2：
Using the equipment described in embodiment 1, it is as follows：
（1）The low-field electrode array of traditional Chinese medicine ingredients extraction equipment shares 20, with upper and lower two panels stainless steel disk such as Fig. 2 institutes
Show that equably arrangement is fixed, and be connected on the lower lid of pulse electric field process chamber and be grounded；20 high-field electrodes are chosen, such as Fig. 3 institutes
Show equably to arrange in a manner of jack and be fixed on the upper lid of electric field pulse electric field processing chamber, high-field electrode and low-field electrode it
Between field gap be 5cm, length 2m；
（2）By Radix Salviae Miltiorrhizae sample cut distribution into the little particle of 1-2mm, the field gap being placed between high-field electrode and low-field electrode
In, and deionized water is added in into head tank；
（3）Connect high-voltage pulse power source, set pulse voltage 80kV, frequency 10Hz, rising edge 300ns, 3.5 μ s of pulsewidth,
Generated electric field strength 16kV/cm in field gap；
（4）Open water pump, deionized water be pumped into the feed inlet of pulse electric field process chamber in intermittent method, by down toward
On with the flow velocity of 0.2m/s by the field gap between high-field electrode and low-field electrode；
（5）Water pump and high-voltage pulse power source alternate run are contacted using in flowmeter control field gap with DANSHEN KELI
Deionized water through 50 sub-high pressure impulse electric fields bombardment after, collecting tank is discharged into together with the tanshinone being extracted；
（6）Product mixtures obtain required drug ingedient after later separation purifies, and find its tanshinone after testing
Leaching rate up to 60%.
Embodiment 3：
Using the equipment described in embodiment 1, it is as follows：
（1）The low-field electrode array of traditional Chinese medicine ingredients extraction equipment shares 20, with upper and lower two panels stainless steel disk such as Fig. 2 institutes
Show that equably arrangement is fixed, and be connected on the lower lid of pulse electric field process chamber and be grounded；20 high-field electrodes are chosen, such as Fig. 3 institutes
Show that threadingly equably arrangement is fixed on the upper lid of electric field pulse electric field processing chamber, high-field electrode and low-field electrode it
Between field gap be 2cm, length 2m；
（2）By Radix Glycyrrhizae sample cut distribution into the thin slice of 1-2mm thickness, the field gap being placed between high-field electrode and low-field electrode
In, and alcohol is added in into head tank；
（3）Connect high-voltage pulse power source, set pulse voltage 100kV, frequency 10Hz, rising edge 300ns, 100 μ s of pulsewidth,
Generated electric field strength 50kV/cm in field gap；
（4）Open water pump, alcohol be pumped into the feed inlet of pulse electric field process chamber in intermittent method, from the bottom to top with
Pass through the field gap between high-field electrode and low-field electrode the flow velocity of 0.2m/s；
（5）Water pump and high-voltage pulse power source alternate run are contacted using in flowmeter control field gap with DANSHEN KELI
Alcohol through 20 sub-high pressure impulse electric fields bombardment after, collecting tank is discharged into together with the glycyrrhizin being extracted；
（6）Product mixtures obtain required drug ingedient after later separation purifies, and find its tanshinone after testing
Leaching rate up to 80%.
Claims (5)
1. a kind of equipment using electroporation technology extraction Chinese medicine drug ingedient, it is characterised in that including head tank, water pump, arteries and veins
Rush electric field processing chamber, collecting tank, high-voltage pulse power source and computer；Wherein,
The pulse electric field process chamber by high-field electrode array, low-field electrode array, upper cover, lower cover, feed inlet, discharge port and
Insulate outer barrel composition, and the upper cover and lower cover are made of an electrically conducting material, and is respectively overlay in above insulation outer barrel and following；Institute
The high-field electrode array stated includes several high-field electrodes, and to be thin rodlike, one end is both secured on lid each high-field electrode, on
The high-pressure side of lid connection high-voltage pulsed source；The low-field electrode array includes several low-field electrodes, and each low-field electrode is
Hollow tubular is fixed into array structure by several metal dish, and low-field electrode is corresponded with high-field electrode with coaxial configuration, low
Piezoelectricity pole is enclosed in outside corresponding high-field electrode, and low-field electrode array one end is connected with lower cover, lower cover ground connection；The charging
Mouth and discharge port are opened in respectively on upper cover or lower lid；In use, field gap of the Chinese medicine between high-field electrode and low-field electrode
Inside it is subject to electric field action；
Described water pump one end is connected with head tank, and the other end is connected with the feed inlet of pulse electric field process chamber；
The discharge port of the pulse electric field process chamber is connected with collecting tank；
The high-pressure side of the high-voltage pulse power source is connected with the upper cover of pulse electric field process chamber, ground terminal and pulse electric field process chamber
Lower cover altogether；
The computer keeps communicating to connect with water pump and high-voltage pulse power source respectively, controls water pump and high-voltage pulse power source
Working condition.
2. the equipment according to claim 1 using electroporation technology extraction Chinese medicine drug ingedient, it is characterised in that institute
For the high-voltage pulse rising edge for the high-voltage pulse power source output stated in below 300ns, pulse width is 1 μ s-100 μ s.
3. the equipment according to claim 1 using electroporation technology extraction Chinese medicine drug ingedient, it is characterised in that institute
Between the high-field electrode array and low-field electrode array stated the spacing of field gap be 0.5-5cm, the high-voltage pulse power source
The pulse voltage of output is 1-100kV；The high-voltage pulse power source output voltage produces between high-field electrode and low-field electrode
Raw electric field strength is in 2-200kV/cm.
4. the equipment according to claim 1 using electroporation technology extraction Chinese medicine drug ingedient, it is characterised in that institute
The high-field electrode array and low-field electrode array stated are uniformly distributed, and dismantled and assembled, the quantity of high-field electrode and low-field electrode according to
The electrical parameter of high-voltage pulse power source and the treating capacity flexible modulation of drug.
5. the equipment according to claim 1 using electroporation technology extraction Chinese medicine drug ingedient, it is characterised in that institute
It states equipment and further includes flowmeter, the flowmeter connects computer, and flowmeter provides the feedback signal of data on flows to computer,
The feedback signal that the computer is provided by flowmeter controls the working condition of water pump and high-voltage pulse power source, makes described
Traditional Chinese medicine ingredients extraction equipment be in continuous duty or service intermittent state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721292484.7U CN207452135U (en) | 2017-10-09 | 2017-10-09 | Utilize the equipment of electroporation technology extraction Chinese medicine drug ingedient |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721292484.7U CN207452135U (en) | 2017-10-09 | 2017-10-09 | Utilize the equipment of electroporation technology extraction Chinese medicine drug ingedient |
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| CN207452135U true CN207452135U (en) | 2018-06-05 |
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ID=62250353
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586715A (en) * | 2017-10-09 | 2018-01-16 | 复旦大学 | Utilize the apparatus and method of electroporation technology extraction Chinese medicine drug ingedient |
| CN114870430A (en) * | 2022-06-20 | 2022-08-09 | 信阳农林学院 | Continuous extraction device and extraction method for traditional Chinese medicine raw materials |
-
2017
- 2017-10-09 CN CN201721292484.7U patent/CN207452135U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586715A (en) * | 2017-10-09 | 2018-01-16 | 复旦大学 | Utilize the apparatus and method of electroporation technology extraction Chinese medicine drug ingedient |
| CN114870430A (en) * | 2022-06-20 | 2022-08-09 | 信阳农林学院 | Continuous extraction device and extraction method for traditional Chinese medicine raw materials |
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