CN207411372U - Liver three-dimensional suspension arrangement - Google Patents
Liver three-dimensional suspension arrangement Download PDFInfo
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- CN207411372U CN207411372U CN201620879148.1U CN201620879148U CN207411372U CN 207411372 U CN207411372 U CN 207411372U CN 201620879148 U CN201620879148 U CN 201620879148U CN 207411372 U CN207411372 U CN 207411372U
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- liver
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Abstract
Liver three-dimensional suspension arrangement, is related to the field of medical instrument technology, and purport is that the liver during the static preservation of liver transplant donor organ and Perfusion preservation hangs and fixes.It includes liver three-dimensional fixed frame, adjustable pipeline positioning device, compartment, liver three-dimensional fixed frame includes top suspension plate, bottom plate and vertical support frame, liver is hung to fixed diaphram, adjustable pipeline positioning device is the universal gooseneck of fixation of one end, it is connected pipeline to fixed liver, compartment is made of temperature control shield and pedestal, and conduit coupling inside and outside compartment is set on pedestal, connects liver and external device for casting.For liver storage in vitro, reparation and culture.
Description
Technical field
The utility model belongs to medical device technical field, is mainly used for the static preservation of liver transplant donor organ and perfusion
Liver suspension during preservation is with fixing.
Background technology
Liver Allograft Preservation has become the research hotspot and emphasis in liver transfer operation field at present.How to come in current liver transfer operation donor
In the case of the shortage of source, it is effectively ensured after the survival of liver transfer operation donor and function and transplanting in effect in patient body,
As liver transfer operation field a great problem.With the development of Liver Allograft Preservation technology, in the presence of the UW liquid at initial stage etc. preserves liquid
Freezen protective（Static Cold Storage, SCS）, develop to cryogenic oxygen and close machine perfusion（Hypothermic
Oxygenated machine perfusion, HOPE）Or even body temperature oxygen closes machine perfusion（Normothermic machine
Perfusion, NMP）, each details that the machine perfusion of liver preserves also is studied more and more deep.
During liver perfusion preservation, if since liver is squeezed, there is luminal stenosis or occlusion in sinus hepaticus, protects
Liquid storage constricted flow, and in input pressure sinus hepaticus expansion can not can be generated in the case of timely and effective adjusting, Perfusion preservation liquid becomes silted up
Product, and with different degrees of swelling of liver cell, denaturation, necrosis.And in Liver Allograft Preservation device, whether liver is squeezed,
There is important association with the fixed form and stressing conditions of liver.In existing various liver machine perfusion save sets, liver
It is the formula of crouching down, has very big defect compared with the three-dimensional stationary state of liver in vivo, it is local in perfusion guarantor is easy to cause liver
Occur ischemic hypoxia necrosis during depositing, decline so as to cause the failure of transplanting or the survival rate of later stage patient.
The patent of Publication No. CN204168947U discloses a kind of New Liver perfusion support equipment and babinet, by liver
It is placed in the pallet with sieve plate, and passes through the connection liver of the interface on side wall and device for casting, can be provided with for liver
The support of effect.But liver only leans on the rigid support of hard medium, and liver internal organizational structure can be caused to generate gravity because of gravity
The mutual extruding of direction from top to down.It is uneven so as to cause being perfused during Perfusion preservation, locally can not effectively it be perfused.Into
And cause the local necrosis that ischaemic-anoxic and toxin occurs in liver and metabolic waste retention induces.Publication No.
The patent of US20110065170 A1 discloses the device that a kind of body temperature preserves liver transfer operation donor, passes through arteria hepatica and vena portae hepatica
Perfusion preservation infiltrates the liver in liquid is preserved, and has accessed arterial oxygenation device in systems, can be carried for Liver Allograft Preservation
For conditions such as suitable pressure, temperature, flow velocitys.But liver is to be positioned over crouching down posture in container, due to the density of liver
More than culture solution, so liver still has the situation mutually squeezed that gravity generates in container and culture solution, influence to fill
Note preservation effect.
The content of the invention
The purpose of this utility model is that changing the fixed form in Liver Allograft Preservation, a kind of machine perfusion is provided and preserves liver
Three-dimensional fixing device and container, construction is reasonable, and principle is simple and practicable or even in suspension and the double action of suspension buoyancy
Under so that liver can be more nearly the natural stress under physiological status in vitro, and each leaf of liver is in what is more unfolded
State enhances the effect of Perfusion preservation.The enough each structural units for smoothly entering liver of Perfusion preservation liquid energy, and smoothly
Liver is flowed out, more fully oxygen and nutrition is carried and is supplied to liver, and the physiological activities such as liver cell metabolism are generated
Waste and toxin preferably take liver out of, improve liver survival and function.The present apparatus enhances the effect of liver machine perfusion preservation
Fruit avoids because sinus hepaticus is expanded caused by liver is squeezed, extends liver machine perfusion holding time, and blood vessel and filling
Pipeline fixing device is noted with good fixation and while anti-twisting effect, but will not extrusion pipe cause narrow, have
Good clinical and trade benefit.
To solve the above problems, the technical solution adopted in the utility model is：
A kind of liver three-dimensional fixing device preserved for liver transplant donor organ static state with Perfusion preservation is provided, it is described
Three-dimensional fixing device includes liver three-dimensional fixed frame, adjustable pipeline positioning device, bearing box.
The liver three-dimensional fixed frame amounts to three parts by top suspension plate, bottom plate and vertical support frame and forms, by 90 ° of conjunctions
Page connection.The top suspension plate is fixed with thick silica gel plate in lower surface, to fixed diaphram；The bottom plate is frame structure,
To stablize entire fixed frame as pedestal, intermediate hollow-out part has pipeline to pass through, and passes through adjustable positioning device and liver three
Fixed frame connection is tieed up to fix.
Fixed form in the utility model simulated liver body, by the silicon that the diaphram above liver is fixed on to frame top
On offset plate, and the ligament connected by liver, liver is suspended in midair.Suspention causes liver close to stress in situ, and
It is adjustable orientable universal that the blood vessel of liver connection and bile duct by coupled perfusion pipeline are fixed on direction and position
On gooseneck, the fixed form of simulated liver in vivo, and pass through and preserve liquid submergence liver so that liver is drawn by buoyancy and suspention
The effect of complete equipilibrium gravity is capable of in the double action of power so that liver is in the state unfolded completely, optimizes preservation effect
Fruit extends the holding time.
The adjustable pipeline positioning device is universal gooseneck, and universal goose is pasted and fixed on by the way that pipeline binding will be perfused
Intrusion pipe road can be made to be positioned at any position and any direction of liver lower part on neck tube, can make disengaging liver by adjusting
Blood vessel and bile duct in the raw, avoid pipeline rotate caused by blood vessel reverse, ensure that the unimpeded of perfusion.
The suspention top plate, comprising silica gel plate, suspending in midair has 8 with the screw of silica gel pad and butterfly nut on plate, lead to
The clamping of silica gel plate and silica gel pad and the fixation of butterfly screw are crossed, diaphram can be firmly secured on suspention top plate,
Achieve the purpose that suspend liver in midair.
To be more nearly connection mode of the liver under natural conditions with body other structures, the liver three-dimensional fixing device
In be provided with the universal gooseneck at adjustable horizontal-upright position and angle of inclination.By cooperation preserve each blood vessel of liver and
The physical slot of bile duct adjusts universal gooseneck position and angle so that and liver vessel and bile duct are in physical slot, and herein
It is fixed to fix liver vessel and bile duct by pair being connected fixed perfusion pipeline with liver vessel and bile duct under state, make
It obtains liver circulation pipeline and is more nearly nature physiological status, optimize liver perfusion preservation condition.
It is reversed for liver vessel is avoided to occur reversing or blood vessel caused by pipeline reverses is perfused, the fixation of the pipeline makes
With the fixed form for pasting binding, i.e., when pipeline being adjusted to optimum position and direction, prevent using with strong viscosity and high temperature resistant
Pipeline is fixed on universal gooseneck by the sterilizing indicator tape of water by way of pasting binding so that pipeline will not fall off or
Person arbitrarily reverses.Which effectively prevents the torsion of blood vessel and caused liver perfusion failure or downright bad.
The bearing box is made of temperature control shield and pedestal two step point.
The temperature control shield is columnar structured, transparent bottomless, energy real-time monitored liver state.Double-layer structure, and be provided with
Intake-outlet, recirculated water between double-layer structure by by external recirculated water bath to adjust liquid and liver temperature in bearing box
Degree.The temperature control shield further includes independent transparent sealing lid, to seal using area after device is completed.
The pedestal is connected with transparent housing, prevents liquid leakage by silicagel pad sealing between pedestal and transparent housing, institute
State pedestal middle position and be provided with several interfaces, for connect arteria hepatica, vena portae hepatica, infrahepatic vena cava, bile duct with it is external
Pipeline is perfused, is equipped with to fix the fixed structure of liver three-dimensional fixed frame on the pedestal.Support leg is installed under pedestal.
The utility model liver three-dimensional fixing device is provided with vena portae hepatica perfusion pipe joint on pedestal, arteria hepatica fills
Note pipe joint, infrahepatic vena cava perfusion pipe joint, bile duct outflow pipe joint, by each interface by liver with it is external
While circumfusion system connects, by Liver Allograft Preservation in the temperature control shield, convenient for providing stable environment, and beneficial to observation.
The utility model liver three-dimensional fixing device provides good stress condition for liver, can be in the static preservation of liver
During, ensure that each structural units of liver is in extended position, circulation low-pressure state is in sinus hepaticus, can effectively be strengthened
Liver Allograft Preservation Solution is for the protecting effect of Microcirculation of Liver structure.
Compared with prior art, the beneficial effects of the utility model are：
The utility model machine perfusion liver three-dimensional fixing device can be to carrying out during liver in vitro Perfusion preservation
It is effectively fixed so that liver is close to the natural stress under physiological status, in suspension and the double action of suspension buoyancy
Under, liver stress more balances, each local effect for being in the state more unfolded, enhancing Perfusion preservation, static
When preserving liver liver micro-structure can be allowed to come into full contact with preservation liquid, ensure that the effect of Liver Allograft Preservation Solution.And by this specially
The liver vessel of profit design and perfusion pipeline fixed form, can be better protected from liver vessel distortion occur so as to influence to fill
Note preserves.In the fixing device bearing box of this patent design, there is the external mechanical cycles perfusion pipeline of connection in bottom device so that
Liver can be preferably connected in fixing device with external perfusion system.And the liver position adjustment side of this patent design
Formula, by fixed liver vessel and the fixed position of silica gel plate of diaphram and the fine tuning of universal gooseneck so that liver by
The position and direction of power state and position and blood vessel and bile duct are more nearly interior state, in liver transfer operation donor Perfusion preservation and quiet
State can preferably preserve liver in preserving.
Specific embodiment
Below by way of specific embodiment, the utility model is described in detail, the utility model is not limited to embodiment and applies
Use scope.
Embodiment
Cardiopulmonary bypass preservation is carried out using fixed rat liver outside the utility model apparatus body.
1st, sterilized 3 times to rat abdomen with Iodophor, rat covered by the clean duck eye towel to sterilize, leaks out abdomen,
The big hole towel in hole is covered.Surgical instrument is set, and the circulator bath for connecting transparent housing is preheated；
2nd, abdominal cavity is opened, skin and hole towel clip are lived with haemostatic clamp, both sides is translated into, portal vein and bile duct is found out with dressing pilers,
The intestines of bile duct are clamped with haemostatic clamp, is that bile duct exposure is clear, is sunken cord with looper, given with the sharp pinhead of 1mL syringes
Bile duct opening is inserted into epidural catheter, the line of embedment is tightened, bile is made to flow out naturally；
3rd, abdominal aorta together with cardinal vein is removed into backbone, is embedded to line, with dressing pilers and elbow tweezer by abdominal aorta with
Infrahepatic vena cava separates, and is stopped with haemostatic clamp, is cut with cell abdominal aorta cutting an osculum, is inserted into silicone tube, knots
It ties up, pays attention to elasticity, same method is intubated infrahepatic vena cava；
4th, thoracic cavity is opened, ligatures superior vena cava on liver, the arch of aorta, clip, before being inserted into woven hose most are clamped with haemostatic clamp
Tubule is held, is tied up, unclamps haemostatic clamp at artery, opens arterial infusion pipe idler wheel, preserving liquid using liver perfusion rinses in liver
Blood；
5th, sunken cord to vena portae hepatica, clip is inserted into 1*2 silicone tubes, tightens line, and branch about ligation opens flow velocity
Idler wheel；
6th, ligation has two, pays attention to not arriving arteria hepatica to the branch of intestines system；
7th, stomach is found, the film that stomach and liver, spleen etc. are adhered is cut off, is penetrated the branch of stomach and spleen with elbow tweezer
Line ligatures, and separation cuts the diaphram between esophagus and liver diaphram, stomach is separated, pays attention to other scissors intestines；
8th, find kidney, ligation separation, the branch of especially right kidney is shorter, thread a needle when sunkening cord it is noted that
9th, fat film covered in abdominal aorta branch etc. with dressing pilers is torn, makes abdominal aorta exposure clear, knot
Prick branch, the wiring on hepatic arterial abdominal aorta is surmounted；
10th, diaphram is completely separated, and ensures the integrality of connective tissue between diaphram and liver, separates liver；
11st, liver appearance is cleaned with 1*PBS；
12nd, liver mass controls：By the detection for entering liver preservation solution He going out liver preservation solution dissolved oxygen, ensure liver oxygen
Consumption is more than 12 every gram of livers of μ g/min；
13rd, diaphram is fixed on by butterfly screw on the silica gel plate of top plate, the sustainer for first finding out wiring is fixed, and is mixed up
Behind direction, whether observation arteria hepatica distorts, and hepatic artery catheterization is bundled in indicator tape on corresponding universal gooseneck；Phase
Vena portae hepatica, bile duct and inferior caval vein are fixed like method.Liver three-dimensional fixed frame is placed in together with liver on pedestal and is fixed, it will be each
Pipeline is connected by the poling on pedestal with the entire circulatory system, and ON cycle, temperature control shield is fixed on pedestal, in temperature control
It is passed through in cover and is preheated to 38 DEG C of Liver Allograft Preservation Solution, until liquid level is more than diaphram position；
14th, the circulatory system is adjusted so that liver is in optimum state；
15th, temperature is adjusted：It is adjusted by the temperature of constant temperature water bath, ensures that liver is in 38 DEG C of constant temperature；
16th, injection pressure is adjusted：Arteria hepatica enters 30% that liver pressure is about liver normal physiological pressure, i.e. 500mmH2O, and
It is about 50 mmH that portal vein, which enters liver pressure,2O, about normal portal vein pressure（110-180 mmH2O）30%-50%；
17th, irrigation flow is adjusted：It adjusts into liver flow, arterial flow is every gram of liver of 0.5mL/min, accounts for and always enters liver flow
50%, portal vein flow is every gram of liver of 0.5mL/min, accounts for and always enters liver flow 50%；
18th, dissolved oxygen is adjusted：Oxygen gas flow rate in bubbling is closed by adjusting oxygen so that the dissolved oxygen value of measure exceeds its range
20mg/L；
19th, pH is adjusted：By adjusting the input ratio of oxygen and carbon dioxide and addition NaHCO3So that culture solution
PH stablizes between 7.35-7.45；
20th, biochemistry detection and blood gas analysis：Superior vena cava efflux on liver is taken per 30min, measures its pH, dissolved oxygen（DO
Value）, salinity, concentration of glucose.20mL effluxes is taken to carry out blood gas analysis, detection project Na per 2h+、K+、Cl-、TCO2、
AnGap、Hct、pH、PCO2、HCO3 -、BUN、BE；
21st, every liver organization is taken to carry out biopsy for 24 hours, liver is taken to the lobe of the liver of different parts, size is 0.5*0.5cm's
Blockage sample, takes extrahepatic duct respectively, is put into the 15mL centrifuge tubes equipped with formaldehyde and preserves.It is cut into slices with sealing wax microtomy,
It is observed under light microscope, takes medium centrifugal detection bacterium pollution condition；
22nd, BrdU cell Proliferations detect：BrdU is added in after the circulatory system connects, is used in culture during sample of tissue
BrdU kits are detected.This experiment kit used is the Super SensitiveTM* of BioGenex productions
Polymer_HRP Detection System kits, operating procedure are carried out according to the flow in kit.If result is in sun
Property, then illustrate that liver cell is being proliferated；
23rd, liver mass controls, and passes through the detection to indices, it is ensured that liver state is without exception and indices just
Often, and BUN detections reach 8 and it is above when replace and all preserve liquid；
24th, liquid will be preserved in system to be replaced, update Liver Allograft Preservation condition every 12h.
Claims (8)
1. a kind of liver three-dimensional suspension arrangement, special including liver three-dimensional fixed frame, adjustable pipeline positioning device, compartment
Sign is：The liver three-dimensional fixed frame is made of top suspension plate, bottom plate and vertical support frame, by 90 ° of hinge connections, according to
The folding of hinge is adjustable as planar structure or frame-shaped construction；The adjustable pipeline positioning device be universal gooseneck, one end
It is fixed on compartment pedestal；The compartment is made of temperature control shield and pedestal, and pipeline connection inside and outside compartment is set on pedestal
Part is connected pipeline is perfused in compartment with external device for casting pipeline.
2. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that adjustable pipeline positioning device：Universal goose
Neck tube position and direction can be adjusted arbitrarily, and can be kept fixed in certain field of load, arteria hepatica, vena portae hepatica, vein,
The livers such as bile duct subsidiary pipeline is bundled by coupled pipeline and universal gooseneck, to adjust the position and direction of blood vessel.
3. compartment according to claim 1 is made of temperature control shield and pedestal, temperature control shield is transparent and removable mutually with pedestal
Unload, artery, portal vein, venous perfusion pipeline and bile duct conduit coupling be provided on pedestal, will in compartment be perfused pipeline with
External device for casting pipeline connection.
4. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that the three-dimensional suspension fixed form of liver：Pass through
Liver three-dimensional fixed frame top suspension plate fixes diaphram, suspends liver in midair using with being connected the ligament of diaphram and liver, and utilizes pipe
Road-universal gooseneck bundled fixed includes but not limited to arteria hepatica, vena portae hepatica, vein, the liver subsidiary pipeline of bile duct and fixes
Liver position.
5. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that：It, will using sticking sterilizing adhesive tape
Pipeline is tied up with universal gooseneck paste together with, pipeline is avoided arbitrarily to twist.
6. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that：Liver does not connect with any hard interface
It touches, liver keeps stress balance by suspension pulling force and perfusion liquid buoyancy and self gravitation in a device.
7. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that：Temperature regulating range residing for liver for-
50 DEG C to 38 DEG C.
8. liver three-dimensional suspension arrangement according to claim 1, it is characterised in that：Bile outflow is provided on device pedestal
Collect sampling pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879148.1U CN207411372U (en) | 2016-08-15 | 2016-08-15 | Liver three-dimensional suspension arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879148.1U CN207411372U (en) | 2016-08-15 | 2016-08-15 | Liver three-dimensional suspension arrangement |
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| Publication Number | Publication Date |
|---|---|
| CN207411372U true CN207411372U (en) | 2018-05-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620879148.1U Expired - Fee Related CN207411372U (en) | 2016-08-15 | 2016-08-15 | Liver three-dimensional suspension arrangement |
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| Country | Link |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362710A (en) * | 2018-11-28 | 2019-02-22 | 广东丁沃生医疗器械有限公司 | Liver perfusion device |
| CN109479872A (en) * | 2018-11-28 | 2019-03-19 | 广东丁沃生医疗器械有限公司 | A kind of liver perfusion consumptive material component |
| CN112772632A (en) * | 2020-12-31 | 2021-05-11 | 重庆北部宽仁医院 | Isolated liver perfusion device |
-
2016
- 2016-08-15 CN CN201620879148.1U patent/CN207411372U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362710A (en) * | 2018-11-28 | 2019-02-22 | 广东丁沃生医疗器械有限公司 | Liver perfusion device |
| CN109479872A (en) * | 2018-11-28 | 2019-03-19 | 广东丁沃生医疗器械有限公司 | A kind of liver perfusion consumptive material component |
| CN109479872B (en) * | 2018-11-28 | 2020-03-10 | 广东丁沃生医疗器械有限公司 | Liver fills consumptive material subassembly |
| CN112772632A (en) * | 2020-12-31 | 2021-05-11 | 重庆北部宽仁医院 | Isolated liver perfusion device |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20190815 |