CN207402841U - Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose - Google Patents

Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose Download PDF

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Publication number
CN207402841U
CN207402841U CN201721541082.6U CN201721541082U CN207402841U CN 207402841 U CN207402841 U CN 207402841U CN 201721541082 U CN201721541082 U CN 201721541082U CN 207402841 U CN207402841 U CN 207402841U
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CN
China
Prior art keywords
plate
viscose
blob
square plate
straight slot
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201721541082.6U
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Chinese (zh)
Inventor
李兵兵
邢亚东
杨鹏飞
刘纯成
李帅帅
刘靓
李双姝
金晶
姚海波
何南江
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huai'an Disease Prevention And Control Center
Original Assignee
Huai'an Disease Prevention And Control Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huai'an Disease Prevention And Control Center filed Critical Huai'an Disease Prevention And Control Center
Priority to CN201721541082.6U priority Critical patent/CN207402841U/en
Application granted granted Critical
Publication of CN207402841U publication Critical patent/CN207402841U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a kind of cutter devices of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, including platform, square plate, stent and rubber knife, square plate is arranged on platform, it and can be along plateau levels linear slide, the glide direction of square plate is provided with graduation mark on platform, the upper end at square plate both ends is laterally vertical and is relatively set with front retainer plate and back striker plate, blob of viscose to be cut is placed between front retainer plate and back striker plate, back striker plate can along square plate towards front retainer plate slide closer or far from, rubber knife is fixed on platform vertically by stent, and the top of the sliding trace positioned at square plate, rubber knife can be moved up and down compared with stent, the blob of viscose to be cut on square plate is cut into required length.The utility model provides a kind of cutter device for the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose that can be fast and accurately cut to a plurality of blob of viscose.

Description

Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose
Technical field
The utility model is related to pulsed field gel electrophoresis field of experiment equipment.More specifically, the utility model is related to one The cutter device of kind pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose.
Background technology
When carrying out Pulse field gel electrophoresis experiment, it is necessary first to which bacterial chromosomal dna is embedded in agarose blob of viscose In, then small glue bacterial chromosomal dna in the block is digested with restriction enzyme, through pulsed field gel electrophoresis, so that greatly The DNA of molecular weight is efficiently separated.Current small adhesive tape by the blob of viscose for being embedded with bacterial chromosomal dna mainly by cutting into required size It obtains, without special equipment, it is necessary to manually cut, but manually goes out to cut high rejection rate, it is less efficient, and due to cutting out Error is larger and influence the resolution ratio of electrophoresis photographs, it is necessary to when being cut into substantial amounts of adhesive tape between different batches adhesive tape, manually cuts The limitation cut just displays.
Utility model content
The purpose of this utility model is to provide a kind of pulsed field gel that can be fast and accurately cut to multiple blob of viscoses Electrophoresis DNA of bacteria embeds the cutter device of blob of viscose
The technical solution that the utility model solves above-mentioned technical problem is as follows:A kind of pulsed field gel electrophoresis DNA of bacteria bag The cutter device of blob of viscose is buried, including platform, square plate, stent and rubber knife, the square plate is set on the platform, and can Along the plateau levels linear slide, the glide direction of the square plate is provided with graduation mark on the platform, it is described square The upper end at plate both ends is laterally vertical and is relatively set with front retainer plate and back striker plate, between the front retainer plate and back striker plate Blob of viscose to be cut is placed, the back striker plate can be slided along the square plate towards the front retainer plate closer or far from institute Rubber knife is stated to fix on the platform vertically by stent, and the top of the sliding trace positioned at the square plate, it is described to cut Glue knife can be moved up and down compared with the stent, and the blob of viscose to be cut on the square plate is cut into required length.
Preferably, in the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the platform is length Square, the upper end is provided with the first straight slot along its length, and the square plate is and the matched rectangle of the first straight slot Plate, and be slidably mounted in first straight slot.
Preferably, in the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, first straight slot Bottom wall on be spaced side by side along its length set there are two the second straight slot, the lower end of the square plate set there are two and institute One-to-one the first square convex block of the second straight slot is stated, it is corresponding described that each first convex block is slidably disposed in its In second straight slot.
Preferably, in the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, first straight slot Bottom wall on be equipped with the 3rd straight slot along its length, one end of the square plate is equipped with positioning corresponding with the 3rd straight slot Plate, the upper of the positioning plate are removably equipped with a square button, and the lower end of the square button through the positioning plate and is connected to In 3rd straight slot, the square plate is fixed on the platform.
Preferably, in the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the square plate Its glide direction interval of upper end edge and opposite setting are there are two bar hole, and there are two the one side fixed settings of the back striker plate With one-to-one second convex block of the bar hole, the lower end of each second convex block is connected to its corresponding bar hole In, the back striker plate is fixed on any position on the square plate.
Preferably, in the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the bar hole is Rectangular blind hole.
The utility model can fast and accurately cut multiple blob of viscoses, avoid the adhesive tape size of single cut generation Individual difference, while improve the efficiency of blob of viscose cutting.
Part is illustrated to embody by the further advantage, target and feature of the utility model by following, and part will also pass through Research and practice to the utility model and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is that the structure for the cutter device that pulsed field gel electrophoresis DNA of bacteria described in the utility model embeds blob of viscose is shown It is intended to;
Fig. 2 is the top view of square plate described in the utility model.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to explanation Book word can be implemented according to this.
It should be noted that in the description of the utility model, term " transverse direction ", " longitudinal direction ", " on ", " under ", " preceding ", The orientation or position relationship of the instructions such as " rear ", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " be based on Orientation shown in the drawings or position relationship, are for only for ease of description the utility model and simplify and describe, and are not instruction or dark Show that signified device or element there must be specific orientation, with specific azimuth configuration and operation, thus it is it is not intended that right The limitation of the utility model.
Fig. 1 is the cutting dress that a kind of pulsed field gel electrophoresis DNA of bacteria that the utility model embodiment provides embeds blob of viscose It puts, including platform 1, square plate 2, stent 3 and rubber knife 4, the square plate 2 is arranged on the platform 1, and can be put down along described 1 horizontal linear of platform slides, and the glide direction of the square plate 2 is provided with graduation mark 5,2 liang of the square plate on the platform 1 The upper end at end is laterally vertical and is relatively set with front retainer plate 21 and back striker plate 22, the front retainer plate 21 and back striker plate 22 Between place blob of viscose to be cut, the back striker plate 22 can slide close along the square plate 2 towards the front retainer plate 21 Or it is separate, the rubber knife 4 is fixed on by stent 3 on the platform 1 vertically, and positioned at the sliding trace of the square plate 2 Top, the rubber knife 4 can move up and down compared with the stent 3, the blob of viscose to be cut on the square plate 2 is cut It is cut into required length.
In above-mentioned technical proposal, when being cut using cutter device to blob of viscose, first according to cutting blob of viscose length, In the position of 2 upper end of square plate movement back striker plate 22 so that the distance between front retainer plate 21 and back striker plate 22 are cut with band The equal length of rubber tapping block, then more blob of viscoses of same specification are placed on front retainer plate 21 along the glide direction of square plate 2 successively Between back striker plate 22, square plate 2 is promoted further along, makes it to movement at rubber knife 4;When blob of viscose is reached at rubber knife 4, Rubber knife 4 is moved up first, makes front retainer plate 21 through the lower section of rubber knife 4, according to graduation mark 5 determine front retainer plate 21 with Distance between projection of the rubber knife 4 on square plate 2, i.e., the length of required blob of viscose, then moves rubber knife 4 still further below, right Blob of viscose on square plate 2 is cut, you can then the adhesive tape of size needed for obtaining lifts rubber knife 4, mobile square plate 2 again To target location, then cut;And in the present solution, as shown in Figure 1, can be on stent 3 along the moving direction of rubber knife 4 Setting and 4 matched groove of rubber knife, rubber knife 4 is clamped in a groove, it is possible to which the cell wall for passing through groove controls rubber knife 4 Trend;As an alternative, rubber knife 4 can be used can be reciprocating such as cylinder mechanical equipment, with improve cut glue Speed.
In the cutter device of the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the platform 1 is rectangle, Upper end is provided with the first straight slot 11 along its length, the square plate 2 be with the 11 matched rectangular slab of the first straight slot, And it is slidably mounted in first straight slot 11.
In another technology in scheme, by being provided with the first straight slot along the length direction of square plate 2 on the platform 1 11, and square plate 2 is arranged in the first straight slot 11, square plate 2 can be connected in the first straight slot 11, can thus caused Square plate 2 moves under external force promotion along the length direction of the first straight slot 11;As an alternative, cylinder or electricity can also be used The mechanical equipments such as machine make square plate 2 be held on the platform 1 towards some scheme in the driving of cylinder or motor and do straight reciprocating motion.
In the cutter device of the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the bottom wall of first straight slot 11 On be spaced side by side along its length set there are two the second straight slot 12, the lower end of the square plate 2 set there are two with it is described Second straight slot, 12 one-to-one the first square convex block 23, it is corresponding that each first convex block 23 is slidably disposed in its In second straight slot 12.
In another technology in scheme, to make square plate 2 is stable in the first straight slot 11 to move along its length, It needs square plate 2 being connected in the first straight slot 11, needs larger strength that square plate 2 could be driven sliding in the first straight slot 11 It is dynamic, in order to make the slip of the first straight slot 11 lighter, it is spaced along its length on the bottom wall of the first straight slot 11 and opposite Setting there are two the second straight slot 12, then set in the lower end of square plate 2 that there are two one-to-one square with the second straight slot 12 The first convex block 23, then the first convex block 23 is slidably disposed in its corresponding second straight slot 12, such square plate 2 will It is slided by the first convex block 23 along the length direction of the first straight slot 11.
In the cutter device of the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the bottom wall of first straight slot 11 On be equipped with the 3rd straight slot 13 along its length, one end of the square plate 2 is equipped with and the 13 corresponding positioning of the 3rd straight slot Plate 6, the upper of the positioning plate 6 are removably equipped with a square button 61, and the lower end of the square button 61 passes through the positioning plate 6 simultaneously It is connected in the 3rd straight slot 13, the square plate 2 is fixed on the platform 1.
When another technology is in scheme, cutting glue, it is necessary to the position of blob of viscose is fixed on 4 lower section of glue knife, in order to prevent It is slided, and need to be fixed the position of square plate 2 after the position of blob of viscose is regulated, sliding during glue is cut to avoid square plate 2 It is dynamic, the accuracy for cutting glue is impacted, so being equipped with the 3rd in this programme along its length on the bottom wall of the first straight slot 11 Straight slot 13, then be equipped in one end of square plate 2 with 13 corresponding positioning plate 6 of the 3rd straight slot, upper in positioning plate 6 is removably set There is a square button 61, the lower end of square button 61 through positioning plate 6 and is connected in the 3rd straight slot 13, square plate 2 is fixed on On the platform 1, and after removing square button 61 from positioning plate 6, square plate 2 can be slided along 1 horizontal linear of platform
As shown in Fig. 2, in the cutter device of the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the square plate 2 Its glide direction interval of upper end edge and opposite setting there are two bar hole 24, the one side of the back striker plate 22 is fixedly installed Its correspondence is connected to there are two the lower end with 24 one-to-one second convex block 25 of bar hole, each second convex block 25 The bar hole 24 in, the back striker plate 22 is fixed on any position on the square plate 2.
In another technology in scheme, since the size difference of different blob of viscoses is, it is necessary to according to blob of viscose size to front The spacing of flitch 21 and back striker plate 22 is adjusted, so at its glide direction interval of the upper end edge of square plate 2 and opposite It sets there are two bar hole 24, then there are two the second convex blocks in the fixed setting of the one side of back striker plate 22 for corresponding two bar holes 24 25, each second convex block 25 is connected in its corresponding bar hole 24, it is possible to which back striker plate 22 is fixed on the square plate 2 On.
In the cutter device of the pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, the bar hole 24 is rectangle Blind hole.
In another technology in scheme, bar hole 24 is arranged to rectangular blind hole can prevent the second convex block 25 Lower end is contradicted with platform 1, and the slip of its square shaped plate 2 is avoided to impact.
Although the embodiment of the utility model is disclosed as above, it is not restricted in specification and embodiment Listed utilization, it can be applied to the field of various suitable the utility model completely, for those skilled in the art, Other modification is easily achieved, therefore without departing from the general concept defined in the claims and the equivalent scope, this reality Specific details is not limited to new and shown here as the embodiment with description.

Claims (6)

1. a kind of cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, including platform (1), square plate (2), stent (3) and rubber knife (4), which is characterized in that the square plate (2) is arranged on the platform (1), and can be along the platform (1) Horizontal linear slides, and the glide direction of the square plate (2) is provided with graduation mark (5), the square plate on the platform (1) (2) upper end at both ends is laterally vertical and is relatively set with front retainer plate (21) and back striker plate (22), the front retainer plate (21) Blob of viscose to be cut is placed between back striker plate (22), the back striker plate (22) can be along the square plate (2) described in Front retainer plate (21) slide closer or far from, the rubber knife (4) is fixed on by stent (3) on the platform (1) vertically, and Positioned at the top of the sliding trace of the square plate (2), the rubber knife (4) can move up and down compared with the stent (3), with Blob of viscose to be cut on the square plate (2) is cut into required length.
2. the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose as described in claim 1, which is characterized in that institute Platform (1) is stated as rectangle, the upper end is provided with the first straight slot (11) along its length, the square plate (2) be with it is described First straight slot (11) matched rectangular slab, and be slidably mounted in first straight slot (11).
3. the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose as claimed in claim 2, which is characterized in that institute Stating setting of being spaced side by side along its length on the bottom wall of the first straight slot (11), there are two the second straight slot (12), the square plates (2) lower end set there are two and one-to-one square the first convex block (23) of second straight slot (12), each described the One convex block (23) is slidably disposed in its corresponding described second straight slot (12).
4. the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose as claimed in claim 2, which is characterized in that institute State on the bottom wall of the first straight slot (11) and to be equipped with the 3rd straight slot (13) along its length, one end of the square plate (2) be equipped with The corresponding positioning plate (6) of 3rd straight slot (13), the upper of the positioning plate (6) are removably equipped with a square button (61), institute The lower end of square button (61) is stated through the positioning plate (6) and is connected in the 3rd straight slot (13), by the square plate (2) it is fixed on the platform (1).
5. such as the cutter device of claim 1-4 any one of them pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose, feature Be, its glide direction interval of the upper end edge of the square plate (2) and opposite setting there are two bar hole (24), it is described after gear The one side fixed setting of flitch (22) is there are two the second convex block (25) one-to-one with the bar hole (24), and each described the The lower end of two convex blocks (25) is connected in its corresponding described bar hole (24), the back striker plate (22) is fixed on described Any position on square plate (2).
6. the cutter device of pulsed field gel electrophoresis DNA of bacteria embedding blob of viscose as claimed in claim 5, which is characterized in that institute Bar hole (24) is stated as rectangular blind hole.
CN201721541082.6U 2017-11-17 2017-11-17 Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose Expired - Fee Related CN207402841U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721541082.6U CN207402841U (en) 2017-11-17 2017-11-17 Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721541082.6U CN207402841U (en) 2017-11-17 2017-11-17 Pulsed field gel electrophoresis DNA of bacteria embeds the cutter device of blob of viscose

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CN207402841U true CN207402841U (en) 2018-05-25

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108858309A (en) * 2018-06-21 2018-11-23 四川省疾病预防控制中心 A kind of pulsed field gel electrophoresis coating pathogen blob of viscose cuts adhesive dispenser

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108858309A (en) * 2018-06-21 2018-11-23 四川省疾病预防控制中心 A kind of pulsed field gel electrophoresis coating pathogen blob of viscose cuts adhesive dispenser

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GR01 Patent grant
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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180525

Termination date: 20191117