CN207391447U - The device that a kind of stem cell layering co-cultures - Google Patents
The device that a kind of stem cell layering co-cultures Download PDFInfo
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- CN207391447U CN207391447U CN201721397129.6U CN201721397129U CN207391447U CN 207391447 U CN207391447 U CN 207391447U CN 201721397129 U CN201721397129 U CN 201721397129U CN 207391447 U CN207391447 U CN 207391447U
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- culture apparatus
- electric cylinder
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- stem cell
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Abstract
The utility model provides the device that a kind of stem cell layering co-cultures,Including upper cover,Seal inner ring,Spring,Sealed outer ring,Culture apparatus main body,Filter membrane,Connecting plate and electric cylinder two,Upper cover lower end is equipped with sealing inner ring,Sealing inner ring lower end is fixed with spring,Inner ring is sealed to be mounted on inside sealed outer ring,Spring is arranged on sealed outer ring inner bottom,Sealing inner ring is connected with sealed outer ring by spring,Upper cover is mounted on sealed outer ring upper end,And it extends to inside sealed outer ring,The design can be sealed culture apparatus main body,Filter membrane is mounted on culture apparatus body interior bottom,Filter membrane outer end is fixed with connecting plate,Connecting plate lower end is fixed with electric cylinder two,Filter membrane,Connecting plate and electric cylinder two are installed in culture apparatus body interior,The design can separate stem cell,The utility model is easy to operate,It is easy to use,Securely and reliably.
Description
Technical field
The utility model is the device that a kind of stem cell layering co-cultures, and belongs to technical field of stem cell culture.
Background technology
Stem cell refers to the initial cell with self-renewing and multi-lineage potential, is the cells of origin of body, in spy
One or more cells for forming tissue or organ can be divided under conditions of fixed, self-renewing refers to that stem cell can be by thin
Born of the same parents divide the size for maintaining stem cell population, and Multidirectional Differentiation refers to that stem cell has plasticity, in different environments, Ke Yifen
Different histocytes is melted into, so as to form the histoorgan of the various complexity of body.
Existing stem cell culturing apparatus does not have separated structure, therefore needs staff first will be dry thin in culture apparatus
Born of the same parents all pour out, and then separate the stem cell broken up, finally by undifferentiated stem cell be put into culture apparatus after
Continuous culture, it is not only cumbersome, it loses time, and is easy to cause stem cell and is contaminated.
Utility model content
In view of the deficienciess of the prior art, the utility model aim is to provide the dress that a kind of stem cell layering co-cultures
It puts, to solve the problems mentioned in the above background technology, the utility model is easy to operate, easy to use, securely and reliably.
To achieve these goals, the utility model is to realize by the following technical solutions：A kind of stem cell layering
The device of co-cultivation, including seal assembly and separation assembly, the separation assembly is mounted on seal assembly lower end, and extends to
Inside seal assembly, the seal assembly includes upper cover, sealing inner ring, spring and sealed outer ring, the upper cover lower end installation
There is sealing inner ring, the sealing inner ring lower end is fixed with spring, and the sealing inner ring is mounted on inside sealed outer ring, the spring
Sealed outer ring inner bottom is arranged on, the sealing inner ring is connected with sealed outer ring by spring, and the upper cover is mounted on close
Outer ring upper end is sealed, and is extended to inside sealed outer ring, the separation assembly includes culture apparatus main body, electric cylinder one, partition plate, mistake
Filter membrane, connecting plate and electric cylinder two, the culture apparatus main body is mounted on upper cover lower end, and extends to inside upper cover, described
Sealed outer ring is mounted on culture apparatus main body outer end, and the inside of the culture apparatus main body is fixed with electric cylinder one, described electronic
One inner end of cylinder is equipped with partition plate, and filter membrane is provided on the downside of the partition plate, and the filter membrane is mounted on culture apparatus body interior
Bottom, the filter membrane outer end are fixed with connecting plate, and the connecting plate lower end is fixed with electric cylinder two, the partition plate, filter membrane,
Connecting plate and electric cylinder two are installed in culture apparatus body interior.
Further, the spring is equipped at least two, and at least two springs are uniformly mounted on inside sealed outer ring
Bottom.
Further, there are two the partition plate and electric cylinder one are all provided with, the electric cylinder one is symmetrically mounted on culture dress
Put body interior.
Further, there are two the electric cylinder two is set, the electric cylinder two is symmetrically mounted on culture apparatus body interior.
Further, the culture apparatus main body right end offers discharge port, and discharge port is arranged on connecting plate right end, and
Sealing-plug is installed inside discharge port.
Further, the upper cover is connected with culture apparatus main body by screw thread, and the electric cylinder one passes through with partition plate
Screw is connected.
The beneficial effects of the utility model：The device that a kind of stem cell layering of the utility model co-cultures, this practicality are new
For type by increasing upper cover, sealing inner ring, spring and sealed outer ring, which, which can realize, is sealed culture apparatus main body
Function, prevent extraneous impurity and bacterium from entering culture dress by the gap between upper cover and culture apparatus body interface
Body interior is put, so as to which the nutrient solution for preventing culture apparatus body interior is contaminated, and then ensure that stem cell can normally divide
Change, during solving cell culture, extraneous impurity and bacterium are easily accessible in existing culture apparatus, so as to dirty
The drawbacks of contaminating culture solution.
The utility model is by increasing culture apparatus main body, electric cylinder one, partition plate, filter membrane, connecting plate and electric cylinder
Two, which can realize separates to differentiated in culture apparatus main body with undifferentiated stem cell, while makes undifferentiated
Stem cell continue in the main In vivo culture of culture apparatus, reduce the operation complexity of staff, while separation can be reduced
The required time, additionally can to avoid in separation process stem cell be contaminated, and then ensure that stem cell can be normal
Differentiation, solving existing staff needs to separate stem cell removal culture apparatus, cumbersome, loses time,
And the easy contaminated problem of stem cell.
Because increasing by least two springs, which can strengthen sealing effect, because increasing by two electric cylinders one and partition plate,
The design can realize layering culture, save space, and because increasing by two electric cylinders two, which can be moved through connecting plate
It keeps stablizing in journey, because increasing sealing-plug, which can prevent discharge port inner wall from adhering to impurity, because increasing screw, the design
Electric cylinder one can be made to be connected fastening with partition plate, the utility model is easy to operate, easy to use, securely and reliably.
Description of the drawings
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other spies of the utility model
Sign, objects and advantages will become more apparent upon：
Fig. 1 is the structure diagram for the device that a kind of stem cell layering of the utility model co-cultures；
Fig. 2 is the schematic diagram of seal assembly in the device that a kind of stem cell layering of the utility model co-cultures；
Fig. 3 is the schematic diagram of separation assembly in the device that a kind of stem cell layering of the utility model co-cultures；
In figure：1- seal assemblies, 2- separation assemblies, 11- upper covers, 12- sealing inner ring, 13- springs, 14- sealed outer rings,
21- culture apparatuses main body, 22- electric cylinders one, 23- partition plates, 24- filter membranes, 25- connecting plates, 26- electric cylinders two.
Specific embodiment
Technical means, creative features, achievable purpose and effectiveness to realize the utility model are easy to understand, below
With reference to specific embodiment, the utility model is expanded on further.
- Fig. 3 is please referred to Fig.1, the utility model provides a kind of technical solution：The device that a kind of stem cell layering co-cultures,
Including seal assembly 1 and separation assembly 2, separation assembly 2 is mounted on 1 lower end of seal assembly, and extends in seal assembly 1
Portion.
Seal assembly 1 includes upper cover 11, sealing inner ring 12, spring 13 and sealed outer ring 14,11 lower end of upper cover and is equipped with
Inner ring 12 is sealed, 12 lower end of sealing inner ring is fixed with spring 13, and sealing inner ring 12 is mounted on inside sealed outer ring 14, and spring 13 is set
It puts in 14 inner bottom of sealed outer ring, sealing inner ring 12 is connected with sealed outer ring 14 by spring 13, and upper cover 11 is mounted on close
14 upper end of outer ring is sealed, and is extended to inside sealed outer ring 14, which can be sealed culture apparatus main body.
Separation assembly 2 includes culture apparatus main body 21, electric cylinder 1, partition plate 23, filter membrane 24, connecting plate 25 and electricity
Dynamic cylinder 2 26, culture apparatus main body 21 is mounted on 11 lower end of upper cover, and extends to inside upper cover 11, and sealed outer ring 14 is mounted on training
Support 21 outer end of apparatus main body, the inside of culture apparatus main body 21 is fixed with electric cylinder 1, one 22 inner end of electric cylinder be equipped with every
Plate 23,23 downside of partition plate are provided with filter membrane 24, and filter membrane 24 is outside 21 inner bottom of culture apparatus main body, filter membrane 24
End is fixed with connecting plate 25, and 25 lower end of connecting plate is fixed with electric cylinder 2 26, partition plate 23, filter membrane 24, connecting plate 25 and electricity
Dynamic cylinder 2 26 is installed in inside culture apparatus main body 21, which can separate stem cell.
Spring 13 is equipped at least two, and at least two springs 13 are uniformly mounted on 14 inner bottom of sealed outer ring, partition plate 23
And there are two electric cylinder 1 is all provided with, electric cylinder 1 is symmetrically mounted on inside culture apparatus main body 21, and electric cylinder 2 26 is set
There are two, electric cylinder 2 26 is symmetrically mounted on inside culture apparatus main body 21, and 21 right end of culture apparatus main body offers discharge port,
And discharge port is arranged on 25 right end of connecting plate, and sealing-plug is installed inside discharge port, upper cover 11 is logical with culture apparatus main body 21
It crosses screw thread to be connected, electric cylinder 1 is connected with partition plate 23 by screw.
Specific embodiment：In progress in use, staff first checks the utility model, check whether and deposit
In defect, it can not just carry out using if there is defect, need that maintenance personal is notified to carry out maintenance at this time, if do not deposited
It can be carried out using if problem, during original state, spring 13 is in compressive state, in use, staff starts electricity
Dynamic cylinder 1, the work band dynamic dividing plate 23 of electric cylinder 1 moves out, when partition plate 23 is moved out to culture apparatus main body 21
When in wall, staff closes electric cylinder 1, then rotary upper cover 11, since upper cover 11 and culture apparatus main body 21 pass through spiral shell
Line is connected, therefore the rotation of upper cover 11 moves upwards simultaneously, while spring 13 occurs to reply deformation, and upper cover 11 is in the reply of spring 13
It is moved upwards under deformation effect, when upper cover 11 is separated with culture apparatus main body 21, staff is by stem cell with cultivating
Liquid is poured into culture apparatus main body 21, is then again started up electric cylinder 1, and then partition plate 23 is made to move to original position, is then worked
Stem cell and culture solution are poured into 23 upper end of partition plate by personnel again, are realized and are carried out layering culture to stem cell, and then staff will
Upper cover 11 goes back to original position, and upper cover 11 moves downward band dynamic sealing inner ring 12 and moves downward, and sealing inner ring 12 is moved downward to spring
13 are compressed, and sealing inner ring 12 can be achieved to compress upper cover 11 under the elastic force effect of spring 13, realize to upper cover 11
Be sealed with 21 interface of culture apparatus main body, which solves during cell culture, extraneous impurity and
Bacterium is easily accessible in existing culture apparatus, the drawbacks of so as to pollute culture solution.
After the completion of stem cell is cultivated, staff starts electric cylinder 2 26, and the work of electric cylinder 2 26 drives connecting plate 25
It moves upwards, connecting plate 25, which moves upwards, drives filter membrane 24 to move upwards, and filter membrane 24, which moves upwards, drives undifferentiated volume
Larger stem cell moves upwards, while the stem cell of differentiated complete small volume is made to remain in culture through filter membrane 24
21 inner bottom of apparatus main body realizes and stem cell is separated, then staff is again started up electric cylinder 1, and then makes
Partition plate 23 move out in 21 inner wall of culture apparatus main body, it can be achieved that being separated to the stem cell of 23 upper end of partition plate, then
Staff moves sealing-plug, and then sealing-plug is made to be separated with discharge port, then staff by discharge port to having divided
The stem cell of change is collected, and then sealing-plug is put back to original position by staff, realizes and culture apparatus main body 21 is sealed,
The design, which solves existing staff, to be needed to separate stem cell removal culture apparatus, cumbersome, is lost time,
And the easy contaminated problem of stem cell.
The basic principle of the utility model and main feature and the advantages of the utility model has been shown and described above, for
For those skilled in the art, it is clear that the utility model is not limited to the details of above-mentioned exemplary embodiment, and without departing substantially from this
In the case of the spirit or essential attributes of utility model, the utility model can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the utility model is by institute
Attached claim rather than above description limit, it is intended that will fall within the meaning and scope of the equivalent requirements of the claims
All changes are embraced therein.Any reference numeral in claim should not be considered as to the involved right of limitation
It is required that.
Moreover, it will be appreciated that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
Using specification as an entirety, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (6)
1. a kind of device that stem cell layering co-cultures, including seal assembly (1) and separation assembly (2), it is characterised in that：Institute
Separation assembly (2) is stated mounted on seal assembly (1) lower end, and it is internal to extend to seal assembly (1)；
The seal assembly (1) includes upper cover (11), sealing inner ring (12), spring (13) and sealed outer ring (14), it is described on
Lid (11) lower end is equipped with sealing inner ring (12), and described sealing inner ring (12) lower end is fixed with spring (13), the sealing inner ring
(12) it is internal to be mounted on sealed outer ring (14), the spring (13) is arranged on sealed outer ring (14) inner bottom, in the sealing
Circle (12) is connected with sealed outer ring (14) by spring (13), and the upper cover (11) is mounted on sealed outer ring (14) upper end, and
It is internal to extend to sealed outer ring (14)；
The separation assembly (2) includes culture apparatus main body (21), electric cylinder one (22), partition plate (23), filter membrane (24), connection
Plate (25) and electric cylinder two (26), the culture apparatus main body (21) is mounted on upper cover (11) lower end, and extends to upper cover
(11) internal, the sealed outer ring (14) is mounted on culture apparatus main body (21) outer end, the culture apparatus main body (21) it is interior
Portion is fixed with electric cylinder one (22), and described one (22) inner end of electric cylinder is equipped with partition plate (23), is set on the downside of the partition plate (23)
There is filter membrane (24), the filter membrane (24) is mounted on culture apparatus main body (21) inner bottom, filter membrane (24) outer end
Connecting plate (25) is fixed with, connecting plate (25) lower end is fixed with electric cylinder two (26), the partition plate (23), filter membrane
(24), it is internal to be installed in culture apparatus main body (21) for connecting plate (25) and electric cylinder two (26).
2. the device that a kind of stem cell layering according to claim 1 co-cultures, it is characterised in that：The spring (13) sets
Have at least two, at least two springs (13) are uniformly mounted on sealed outer ring (14) inner bottom.
3. the device that a kind of stem cell layering according to claim 1 co-cultures, it is characterised in that：The partition plate (23) with
And there are two electric cylinder one (22) is all provided with, it is internal that the electric cylinder one (22) is symmetrically mounted on culture apparatus main body (21).
4. the device that a kind of stem cell layering according to claim 1 co-cultures, it is characterised in that：The electric cylinder two
(26) there are two setting, it is internal that the electric cylinder two (26) is symmetrically mounted on culture apparatus main body (21).
5. the device that a kind of stem cell layering according to claim 1 co-cultures, it is characterised in that：The culture apparatus master
Body (21) right end offers discharge port, and discharge port is arranged on connecting plate (25) right end, and sealing-plug is equipped with inside discharge port.
6. the device that a kind of stem cell layering according to claim 1 co-cultures, it is characterised in that：The upper cover (11) with
Culture apparatus main body (21) is connected by screw thread, and the electric cylinder one (22) is connected with partition plate (23) by screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721397129.6U CN207391447U (en) | 2017-10-26 | 2017-10-26 | The device that a kind of stem cell layering co-cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721397129.6U CN207391447U (en) | 2017-10-26 | 2017-10-26 | The device that a kind of stem cell layering co-cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207391447U true CN207391447U (en) | 2018-05-22 |
Family
ID=62326618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721397129.6U Active CN207391447U (en) | 2017-10-26 | 2017-10-26 | The device that a kind of stem cell layering co-cultures |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207391447U (en) |
-
2017
- 2017-10-26 CN CN201721397129.6U patent/CN207391447U/en active Active
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