CN106281972A - A kind of legal medical expert's sample DNA extracts test kit - Google Patents
A kind of legal medical expert's sample DNA extracts test kit Download PDFInfo
- Publication number
- CN106281972A CN106281972A CN201610911192.0A CN201610911192A CN106281972A CN 106281972 A CN106281972 A CN 106281972A CN 201610911192 A CN201610911192 A CN 201610911192A CN 106281972 A CN106281972 A CN 106281972A
- Authority
- CN
- China
- Prior art keywords
- test kit
- box body
- lid
- upper chamber
- sample dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
nullThe present invention relates to test kit technical field，Especially a kind of legal medical expert's sample DNA extracts test kit，Including box body and the lid being hinged on box body，Separated by dividing plate in described box body and have upper chamber and lower chambers，Described dividing plate is provided with multiple placing trough，The lower end of multiple described placing troughs is all run through dividing plate and is positioned at bottom chamber，The inwall of described upper chamber is movably installed with rubber sealing film，And the inwall laminating of rubber sealing film and multiple placing troughs，Described lid is provided with the multiple fixing devices corresponding with multiple placing troughs near the side of box body，Each described fixing device includes the spring being fixedly mounted on lid，Described spring is positioned at the surface of placing trough，And the internal diameter of spring is all higher than the internal diameter of placing trough，Rubber blanket all it is enclosed with on each described spring，The present invention efficiently solves test kit and is not easily cleaned when being infected with in reagent bottle the reagent overflowed，And reagent bottle fixing firm not problem in test kit，Use convenient.
Description
Technical field
The present invention relates to test kit technical field, particularly relate to a kind of legal medical expert's sample DNA and extract test kit.
Background technology
Sample DNA extracts premise and the key being to obtain sample gene information.A kind of optimal extracting method can be passed through
Obtain high-quality and obtain greater number of nucleic acid molecules, decide amplification success.Now widely used extracting method
Having divalent ion chelating method, organic solvent method, alkaline lysis etc., these methods are the longest, and operate complexity, lose sample
This DNA is the most more.Additionally can not effectively remove the impurity in sample, cause expanding unsuccessfully.Meanwhile, these methods produce a large amount of
Toxic waste liquid, to human body and environmental danger.Comparing above-mentioned nucleic acid extraction technology, Magnet Treatment is a kind of simple effective
Nucleic acid purification method.The magnetic bead surfaces that the method uses is connected to the functional group that can have an effect specifically with DNA, has
The characteristic of reversible adsorption DNA.The advantage of paramagnetic particle method purifying nucleic acid maximum is automatization, and the reagent provided can be used for magnetic work
Tool, this kind of method need not any organic solvent, it is not required that is centrifuged repeatedly, and vacuum filters or the step such as post separation, is only
Combine based on magnetic powder by nucleic acid, greatly reduce the test harm to staff, and reduce the spy to testing equipment
Different requirement.But the current test kit design using paramagnetic particle method to carry out DNA extraction is not reasonable, in use exists the most not
Just.Test kit is not easily cleaned when being infected with in reagent bottle the reagent overflowed, and reagent bottle is fixing firm not in test kit, can
Can cause the damage of DNA sample, to this, we release a kind of legal medical expert's sample DNA and extract test kit.
Summary of the invention
The invention aims to solve shortcoming present in prior art, and a kind of legal medical expert's sample DNA proposed carries
Take test kit.
To achieve these goals, present invention employs following technical scheme:
A kind of legal medical expert's sample DNA of design extracts test kit, including box body and the lid being hinged on box body, passes through in described box body
Dividing plate separates has upper chamber and lower chambers, described dividing plate to be provided with multiple placing trough, and the lower end of multiple described placing troughs is all run through
Dividing plate is positioned at bottom chamber, and the inwall of described upper chamber is movably installed with rubber sealing film, and rubber sealing film and multiple placements
The inwall laminating of groove, described lid is provided with the multiple fixing devices corresponding with multiple placing troughs near the side of box body, each
Described fixing device includes the spring being fixedly mounted on lid, and described spring is positioned at the surface of placing trough, and spring is interior
Footpath is all higher than the internal diameter of placing trough, and each described spring is all enclosed with rubber blanket.
Preferably, described bottom chamber is provided with refrigerant solution, and the side of lower chambers is respectively equipped with water filling port and draining
Mouthful.
Preferably, described dividing plate is copper with placing trough and becomes.
Preferably, described lid is provided with for powering to LED in the side of box body is additionally provided with LED, and lid
Accumulator, described lid is additionally provided with into accumulator charging interface.
Preferably, described lid is provided with the projection being flexibly connected with upper chamber inwall near the side of box body.
Preferably, the side that described rubber sealing film fits with upper chamber is provided with the lining identical with upper chamber inner wall shape
Plate, and rubber sealing film is movably arranged in upper chamber by liner plate.
Preferably, described liner plate and the inwall of upper chamber are all fixed with magnetic stripe, and liner plate is logical with the inwall of upper chamber
Cross the mutual adhesive of magnetic stripe.
A kind of legal medical expert's sample DNA that the present invention proposes extracts test kit, has the beneficial effects that: by rubber sealing film by upper
Chamber and placing trough seal, and prevent the reagent of reagent bottle body from overflowing and being infected with upper chamber's inwall and placing trough, and rubber is close
Sealer employing activity is installed, and can be taken off being carried out, and the part that rubber sealing film and placing trough are fitted can be opened and is carried out, and makes
Clean more convenient, the upper-end contact of test kit intrinsic cleaning, rubber blanket and reagent bottle body is effectively ensured, by spring
Pressure compaction reagent bottle body, makes reagent bottle body more stable.
Accompanying drawing explanation
Fig. 1 is the structural representation that a kind of legal medical expert's sample DNA that the present invention proposes extracts test kit；
Fig. 2 is the sectional structure schematic diagram that a kind of legal medical expert's sample DNA that the present invention proposes extracts test kit.
In figure: box body 1, lid 2, dividing plate 3, placing trough 4, fixing device 5, upper chamber 6, lower chambers 7, rubber sealant 8,
Spring 9, rubber blanket 10, LED 11, projection 12.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.
With reference to Fig. 1-2, a kind of legal medical expert's sample DNA extracts test kit, including box body 1 and the lid 2 being hinged on box body 1,
Being separated by dividing plate 3 in box body 1 and have upper chamber 6 and lower chambers 7, dividing plate 3 is provided with multiple placing trough 4, multiple placing troughs 4 times
End all runs through dividing plate 3 and is positioned at lower chambers 7, is used for placing reagent bottle body, arranges upper chamber 6 and make reagent bottle body in placing trough
The exposed upper chamber in upper part 6 in, make to take and place reagent bottle body more convenient, the inwall of upper chamber 6 is movably installed with
Rubber sealing film 8, and the inwall laminating of rubber sealing film 8 and multiple placing troughs 4, and put upper chamber 6 by rubber sealing film 8
Put groove 4 to seal, prevent the reagent of reagent bottle body from overflowing and being infected with upper chamber 6 inwall and placing trough 4, rubber sealing film 8
Employing activity is installed, and can be taken off being carried out, and the part that rubber sealing film 8 and placing trough 4 are fitted can be opened and is carried out, and makes clear
Wash more convenient, test kit intrinsic cleaning is effectively ensured, in lower chambers 7, be provided with refrigerant solution, and the side of lower chambers 7 is divided
Not being provided with water filling port and discharge outlet, refrigerant solution is for reducing the temperature of lower chambers 7, thus ensures this internal DNA of reagent bottle
The storage condition of sample, is replaced refrigerant solution by water filling port and discharge outlet, and dividing plate 3 and placing trough 4 are copper
Becoming, dividing plate 3 and placing trough 4 temperature conductivity that copper becomes are more preferable.
Lid 2 is provided with the multiple fixing devices 5 corresponding with multiple placing troughs 4, each fixing dress near the side of box body 1
Putting 5 springs 9 including being fixedly mounted on lid 2, spring 9 is positioned at the surface of placing trough 4, and the internal diameter of spring 9 is all higher than
The internal diameter of placing trough 4, each spring 9 is all enclosed with rubber blanket 10, and spring 9 is socketed in the upper end of reagent bottle body, passes through rubber
Rubber cushion 10 and the upper-end contact of reagent bottle body, by the pressure compaction reagent bottle body of spring 9, make reagent bottle body more steady
Fixed, lid 2 is provided with the accumulator for powering to LED 11 in the side of box body 1 is additionally provided with LED 11, and lid 2,
Being additionally provided with the interface into accumulator charging on lid 2, LED 11 is for being illuminated work in box body 1, it is simple to box body 1
The work being inside sampled, lid 2 is provided with the projection 12 being flexibly connected with upper chamber 6 inwall near the side of box body 1, and protruding 12
For sealing box body 1 and lid 2, making sealing box body 1 and lid 2 that the situation of seepage will not occur, make better tightness, rubber is close
Sealer 8 is provided with the liner plate identical with upper chamber 6 inner wall shape with the side that upper chamber 6 fits, and rubber sealing film 8 is by lining
Plate is movably arranged in upper chamber 6, makes the shape of rubber sealing film 8 identical with the interior wall construction of upper chamber 6 by liner plate, it is simple to
The dismounting of rubber sealing film 8 and installation, use more convenient, and liner plate is all fixed with magnetic stripe, and liner plate on the inwall of upper chamber 6
With the inwall of upper chamber 6 by the mutual adhesive of magnetic stripe, make liner plate more stable with the connection of upper chamber 6, thus rubber seal can be made
Film 8 is more stable, and is readily disassembled.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (7)
1. legal medical expert's sample DNA extracts a test kit, including box body (1) and the lid (2) that is hinged on box body (1), its feature
Being, being separated by dividing plate (3) in described box body (1) has upper chamber (6) and lower chambers (7), and described dividing plate (3) is provided with multiple
Placing trough (4), the lower end of multiple described placing troughs (4) all runs through dividing plate (3) and is positioned at lower chambers (7), described upper chamber (6)
Inwall is movably installed with rubber sealing film (8), and the inwall laminating of rubber sealing film (8) and multiple placing troughs (4), described lid
(2) side near box body (1) is provided with the multiple fixing devices (5) corresponding with multiple placing troughs (4), each described fixing dress
Putting the spring (9) that (5) include being fixedly mounted on lid (2), described spring (9) is positioned at the surface of placing trough (4), and spring
(9) internal diameter is all higher than the internal diameter of placing trough (4), and each described spring (9) is all enclosed with rubber blanket (10).
A kind of legal medical expert's sample DNA the most according to claim 1 extracts test kit, it is characterised in that in described lower chambers (7)
It is provided with refrigerant solution, and the side of lower chambers (7) is respectively equipped with water filling port and discharge outlet.
A kind of legal medical expert's sample DNA the most according to claim 1 extracts test kit, it is characterised in that described dividing plate (3) and putting
Put groove (4) and be copper.
A kind of legal medical expert's sample DNA the most according to claim 1 extracts test kit, it is characterised in that described lid (2) is close
The side of box body (1) is provided with the accumulator for powering to LED (11) in being additionally provided with LED (11), and lid (2), described
The interface into accumulator charging it is additionally provided with on lid (2).
A kind of legal medical expert's sample DNA the most according to claim 1 extracts test kit, it is characterised in that described lid (2) is close
The side of box body (1) is provided with the projection (12) being flexibly connected with upper chamber (6) inwall.
A kind of legal medical expert's sample DNA the most according to claim 1 extracts test kit, it is characterised in that described rubber sealing film
(8) it is provided with the liner plate identical with upper chamber (6) inner wall shape with the side that upper chamber (6) fits, and rubber sealing film (8) leads to
Cross liner plate to be movably arranged in upper chamber (6).
A kind of legal medical expert's sample DNA the most according to claim 6 extracts test kit, it is characterised in that described liner plate and epicoele
All it is fixed with magnetic stripe on the inwall of room (6), and the inwall of liner plate and upper chamber (6) is by the mutual adhesive of magnetic stripe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610911192.0A CN106281972A (en) | 2016-10-19 | 2016-10-19 | A kind of legal medical expert's sample DNA extracts test kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610911192.0A CN106281972A (en) | 2016-10-19 | 2016-10-19 | A kind of legal medical expert's sample DNA extracts test kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106281972A true CN106281972A (en) | 2017-01-04 |
Family
ID=57719721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610911192.0A Pending CN106281972A (en) | 2016-10-19 | 2016-10-19 | A kind of legal medical expert's sample DNA extracts test kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106281972A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106947690A (en) * | 2017-02-28 | 2017-07-14 | 新乡医学院第附属医院 | A kind of kit for detecting or aiding in detection tuberculous pleurisy |
| CN111498254A (en) * | 2020-04-24 | 2020-08-07 | 上海康与生物科技有限公司 | Gene amplification detection kit |
-
2016
- 2016-10-19 CN CN201610911192.0A patent/CN106281972A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106947690A (en) * | 2017-02-28 | 2017-07-14 | 新乡医学院第附属医院 | A kind of kit for detecting or aiding in detection tuberculous pleurisy |
| CN111498254A (en) * | 2020-04-24 | 2020-08-07 | 上海康与生物科技有限公司 | Gene amplification detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170104 |
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| WD01 | Invention patent application deemed withdrawn after publication |