CN207366314U - A kind of separate type sample pre-treatment device - Google Patents
A kind of separate type sample pre-treatment device Download PDFInfo
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- CN207366314U CN207366314U CN201721008009.2U CN201721008009U CN207366314U CN 207366314 U CN207366314 U CN 207366314U CN 201721008009 U CN201721008009 U CN 201721008009U CN 207366314 U CN207366314 U CN 207366314U
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- 238000002203 pretreatment Methods 0.000 title claims abstract description 33
- 239000000523 sample Substances 0.000 claims abstract description 239
- 230000007246 mechanism Effects 0.000 claims abstract description 90
- 239000007788 liquid Substances 0.000 claims abstract description 71
- 238000006243 chemical reaction Methods 0.000 claims abstract description 53
- 238000004140 cleaning Methods 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 25
- 238000005119 centrifugation Methods 0.000 claims description 13
- 239000006166 lysate Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 238000011534 incubation Methods 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 21
- 230000008569 process Effects 0.000 abstract description 18
- 238000002360 preparation method Methods 0.000 abstract description 9
- 238000004458 analytical method Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 239000008280 blood Substances 0.000 description 20
- 210000004369 blood Anatomy 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 9
- 108010006464 Hemolysin Proteins Proteins 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 239000003228 hemolysin Substances 0.000 description 8
- 239000002699 waste material Substances 0.000 description 8
- 230000008450 motivation Effects 0.000 description 7
- 230000035939 shock Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000012864 cross contamination Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of separate type sample pre-treatment device, it has：Sample disk module, including sample disk and the first rotary drive mechanism, sample disk are equipped with sample tube seat；Antibody disk module, including antibody disk and the second rotary drive mechanism, antibody disk are equipped with antibody tube seat；First liquid relief arm module, including the first probe, first mechanical arm, the first driving mechanism and the second driving mechanism；Second liquid relief arm module, including the second probe, second mechanical arm, the 3rd driving mechanism and the 4th driving mechanism；Disk module, including centrifugal pan and the 3rd rotary drive mechanism are centrifuged, centrifugal pan is equipped with reaction pipe holder, and reaction pipe holder is equipped with reaction tube seat；3rd liquid relief arm module, including the 3rd probe, three-mechanical arm, the 5th driving mechanism and with the 6th driving mechanism.The device can complete the cell preparation work of sample before loading analysis, can largely save manually but also improve sample process effect.
Description
Technical field
The utility model belongs to cytologic technology field, and in particular to a kind of separate type sample pre-treatment device.
Background technology
Flow cytometry (Flow Cytometry, FCM) is that one kind can quickly survey cell or subcellular structure
The new analytical technology and sorting technology of amount.Its main feature is that：1. measuring speed is fast, can most be measured soon at 1 second in kind tens thousand of thin
Born of the same parents；2. measuring multiple parameters can be carried out, the measuring multiple parameters in relation to Wuli-Shili-Renli system approach can be done to same cell, and have
Obvious statistical significance；3. being a comprehensive high-tech approach, it combines laser technology, computer technology, fluid
Mechanics, cytochemistry, image technique etc. are from multi-field knowledge and achievement；4. it is both cell analysis technology and accurate point
Selecting technology.It is that cell sample prepares quality to influence one of key factor of flow cytometry success or failure.It is thin to be presently used for streaming
The cell sample of born of the same parents' instrument detection, it is most of at home to examine section office to rely primarily on manual preparation.
But had the following disadvantages using cell sample is prepared by hand：
1st, the sample added by hand is relatively more, wastes sample resource.
2nd, the antibody added by hand is more or less, and antibody is valuable, and there are waste or deficiency, influences machine on final gained sample
Testing result.
3rd, manual preparation process easily malfunctions.
4th, manual preparation time length, efficiency are low.
5th, prepare by hand and rely on operating experience, nobody is available for domestic many section office, there are flow cytometer in some section office, but not
Sample can be prepared, to lean on service provider to provide sample preparation service.
6th, manual operations can not realize great amount of samples preparation work.
7th, the cell concentration uniformity of the cell sample prepared by hand is poor.
Utility model content
The purpose of the utility model is to overcome above-mentioned the deficiencies in the prior art, there is provided one kind can be on flow cytometer
The separate type sample pre-treatment device of sample preparation is performed before machine analysis in a manner of full automation.
The purpose of this utility model is achieved through the following technical solutions：
A kind of separate type sample pre-treatment device, including：
Sample disk module, including sample disk and for driving rotating first rotary drive mechanism of the sample disk, it is described
Sample disk is circumferentially equidistantly arranged with multiple sample tube seats；
Antibody disk module, including antibody disk and the second rotary drive mechanism for driving the antibody disc spins, it is described
Antibody disk is circumferentially equidistantly arranged with multiple antibody tube seats；
First liquid relief arm module, including the first probe, the first mechanical arm for fixing first probe, for driving
Rotating first driving mechanism of the first mechanical arm and the second driving machine for driving the first mechanical arm to move up and down
Structure, first probe are used to draw and fill antibody and sample；
Second liquid relief arm module, including the second probe, the second mechanical arm for fixing second probe, for driving
Rotating 3rd driving mechanism of the second mechanical arm and the 4th driving machine for driving the second mechanical arm to move up and down
Structure, second probe are used to fill lysate and buffer solution；
Disk module, including centrifugal pan and for driving rotating 3rd rotary drive mechanism of the centrifugal pan are centrifuged, it is described
Centrifugal pan is circumferentially equidistantly arranged with multiple reaction pipe holders, and at least one reaction is provided with each reaction pipe holder
Tube seat, the centrifugal pan be used for realization the sample, the antibody, the lysate and the buffer solution incubation, mix and
Centrifugation, the 3rd rotary drive mechanism are used to drive the centrifugal pan realization to rotate clockwise or counterclockwise and be accurately positioned；
3rd liquid relief arm module, including the 3rd probe, the three-mechanical arm for fixing the 3rd probe, for driving
Rotating 5th driving mechanism of the three-mechanical arm and the 6th driving machine for driving the three-mechanical arm to move up and down
Structure, the 3rd probe are used to aspirate the supernatant after centrifugation.
Further, the separate type sample pre-treatment device further includes cleaning module, and the cleaning module includes cleaning
Pump, syringe pump, solenoid valve, fluid path pipeline, service sink and cleaning solution bucket, first probe, second probe and described
Three probes are connected with the syringe pump respectively, and the syringe pump is connected by the solenoid valve with the cleaning pump, the cleaning
Pump intake is connected with the cleaning solution bucket, and the cleaning pump discharge is connected by the fluid path pipeline with the service sink.
Further, the service sink includes the first service sink, the second service sink and the 3rd service sink, first cleaning
Pond is arranged on below first probe, and second service sink is arranged on below second probe, the 3rd service sink
It is arranged on below the 3rd probe.
Further, the centrifugal pan makes the sample by the vibrations of itself and the liquid impulse force of the filling lysate
Originally, the antibody and the lysate mix.
Further, the 3rd rotary drive mechanism side is provided with for reducing the slow of the centrifugal pan vibration amplitude
Rush bearing.
Further, the first mechanical arm is driven with first driving mechanism and described second successively by first rotating shaft
Motivation structure connects, in the distance between the first rotating shaft and the sample disk center and the first rotating shaft and the antibody disk
The distance between heart is equal.
Further, the first liquid level sensor is provided with the first mechanical arm, first liquid level sensor is used for
Automatically the liquid level of antibody in the antibody pipe is detected, the second liquid level sensor is provided with the three-mechanical arm, it is described
Second liquid level sensor is used for the liquid level for detecting supernatant in the reaction tube automatically.
Further, the outside of the antibody disk is provided with the first temperature control cavity, and first temperature control cavity is used to make described anti-
The temperature of body disc maintains 2~8 DEG C；The outside of the centrifugal pan is provided with the second temperature control cavity, and second temperature control cavity is used to make
The reaction temperature of the centrifugal pan maintains 37 DEG C.
Further, sample tube is placed with the correspondence position of the sample tube seat, the sample tube is used to contain described
Sample；Antibody pipe is placed with the correspondence position of the antibody tube seat, the antibody pipe is used to contain the antibody；The reaction
Reaction tube is placed with the correspondence position of tube seat, the sample, the antibody and the lysate are loaded with the reaction tube
The reaction solution of formation.
Further, the separate type sample pre-treatment device further includes operation control module, the operation control module
For control the sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module,
The action of the 3rd liquid relief arm module and the centrifugation disk module.
Optionally, the separate type sample pre-treatment device further includes scan sample module, and the scan sample module is set
It is placed on the outside of the sample tube, and is connected with the operation control module, can be put into by scanning on the outside of the sample tube two
Code is tieed up, feeds back to the operation control module, the experiment flow of the sample tube is controlled by the operation control module.
The utility model has the advantages that：
The utility model provides a kind of separate type sample pre-treatment device, which can be good at overcoming artificial treatment
The shortcomings that bringing, completes the cell preparation work of blood sample before machine analysis on flow cytometer, can largely save manually again
Blood sample treatment effect can be improved.
Other features and advantages of the utility model will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
, below will be right in order to illustrate more clearly of the utility model embodiment or technical solution of the prior art and advantage
Attached drawing required for describing the embodiments or the prior art is briefly described, it should be apparent that, it is attached in describing below
Figure is only some embodiments of the utility model, for those of ordinary skill in the art, is not being made the creative labor
On the premise of, other attached drawings can also be obtained according to these attached drawings.
Fig. 1 is the dimensional structure diagram of separate type sample pre-treatment device provided by the utility model；
Fig. 2 is the internal structure schematic diagram of separate type sample pre-treatment device provided by the utility model；
Fig. 3 is the front view of separate type sample pre-treatment device provided by the utility model；
Fig. 4 is the top view of separate type sample pre-treatment device provided by the utility model；
Fig. 5 is the dimensional structure diagram of sample disk module provided by the utility model；
Fig. 6 is the dimensional structure diagram of antibody disk module provided by the utility model；
Fig. 7 is the dimensional structure diagram of centrifugation disk module provided by the utility model；
In figure：1- sample disks, the first rotary drive mechanisms of 2-, 3- sample tube seats, 4- antibody disks, the second rotary driving machines of 5-
Structure, 6- antibody tube seats, the first probes of 7-, 8- first mechanical arms, the first driving mechanisms of 9-, the second driving mechanisms of 10-, 11- second
Probe, 12- second mechanical arms, the 3rd driving mechanisms of 13-, 14- centrifugal pans, the 3rd rotary drive mechanisms of 15-, 16- reaction tube branch
Frame, 17- reaction tube seats, the 3rd probes of 18-, 19- three-mechanical arms, the 5th driving mechanisms of 20-, the 6th driving mechanisms of 21-, 22-
First service sink, the second service sinks of 23-, the 3rd service sinks of 24-, 25- cushioning support-saddles, 26- first rotating shafts, the second shafts of 27-,
The 3rd shafts of 28-, the first temperature control cavities of 29-, the second temperature control cavities of 30-, 31- touch-screens, 32- instrument hosts.
Embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the premise of creative work
Lower all other embodiments obtained, shall fall within the protection scope of the present invention.
In the description of the utility model, it is to be understood that the orientation of the instruction such as term " on ", " under ", " interior ", " outer "
Or position relationship is based on orientation shown in the drawings or position relationship, is for only for ease of description the utility model and simplification is retouched
State, rather than the device or element of instruction or hint meaning there must be specific orientation, with specific azimuth configuration and behaviour
Make, therefore it is not intended that limitation to the utility model.
In the utility model, unless otherwise clearly defined and limited, the term such as term " connection " should be interpreted broadly,
For example, it may be fixedly connected or be detachably connected, or integrally；Can mechanically connect or be electrically connected
Connect；It can make to be connected directly, can also be indirectly connected by intermediary, can be the connection inside two elements or two members
The interaction relationship of part.For the ordinary skill in the art, it can understand that above-mentioned term exists as the case may be
Concrete meaning in the utility model.In addition, term " comprising " and " having " and their any deformation, it is intended that covering
Non-exclusive includes, and is not necessarily limited to clearly for example, containing the process of series of steps or unit, method, apparatus, product or equipment
Those steps or unit listed to Chu, but may include not list clearly or for these processes, method, product or
The intrinsic other steps of equipment or unit.
Embodiment one
A kind of separate type sample pre-treatment device is present embodiments provided, which is suitable for the processing of whole blood sample.
As shown in Figures 1 to 4, separate type sample pre-treatment device provided in this embodiment includes：
Sample disk module, as shown in figure 5, including sample disk 1 and the first rotation driving being arranged on below the sample disk 1
Mechanism 2, first rotary drive mechanism 2 are used to drive the sample disk 1 to rotate.Preferably, the sample disk 1 is disk
Shape, the sample disk 1 are circumferentially equidistantly arranged with 25 sample tube seats 3, and one is placed with each sample tube seat 3
Sample tube, is each loaded with blood sample described in 50-150 μ L in the sample tube.
Antibody disk module, as shown in fig. 6, including antibody disk 4 and the second rotation driving being arranged on below the antibody disk 4
Mechanism 5, second rotary drive mechanism 5 are used to drive the antibody disk 4 to rotate.Preferably, the antibody disk 4 is disk
Shape, the antibody disk 4 are circumferentially equidistantly arranged with 25 antibody tube seats 6, and one is placed with each antibody tube seat 6
Antibody pipe, is each loaded with 5-25 μ L antibody in the antibody pipe.Preferably, the outside of the antibody disk 4 is provided with the first temperature
Chamber 29 is controlled, first temperature control cavity 29 is used to make the temperature of the antibody disk 4 maintain 2~8 DEG C, so as to extend the antibody
Service life.
First liquid relief arm module, including the first probe 7, the first mechanical arm 8 for fixing first probe 7, be used for
Drive rotating first driving mechanism 9 of the first mechanical arm 8 and second for driving that the first mechanical arm 8 moves up and down
Driving mechanism 10.The first mechanical arm 8 is driven with first driving mechanism 9 and described second successively by first rotating shaft 26
Mechanism 10 connects, and the first rotating shaft 26 is located at the right side of the sample disk 1 and the antibody disk 4, and the first rotating shaft 26
The distance between described 1 center of sample disk and the first rotating shaft 26 are equal with the distance between 4 center of antibody disk.It is excellent
Choosing, the first mechanical arm 8 has positional fault warning function, when position occurs in 8 motion process of first mechanical arm
During mistake, alarm signal is sent.First probe 7 is applied not only to draw the antibody and the blood sample, is additionally operable to quantitative
Fill the antibody and the blood sample.First probe 7 has anti-collision, when first probe 7 is subject to vertically
It can be automatically stopped when the resistance in direction or shock, and send alarm signal.It is worth noting that, the internal diameter of first probe 7
Should not it is too big also should not be too small, if excessive, the precision of antibody described in compressing can be caused to be not allowed；If too small, can cause described in compressing
Less cleaning position after blood sample and stifled pin.In order to avoid the antibody wastes, the first liquid level is provided with the first mechanical arm 8
Sensor (not shown), for detecting the liquid level of the antibody in the antibody pipe automatically, ensures that the antibody is maximum
Degree blots.
Second liquid relief arm module, including the second probe 11, the second mechanical arm 12 for fixing second probe 11, use
In rotating 3rd driving mechanism 13 of the drive second mechanical arm 12 and for driving the second mechanical arm 12 to move up and down
The 4th driving mechanism.The second mechanical arm 12 by the second shaft 27 successively with the 3rd driving mechanism 13 and described
Four driving mechanisms connect, and second shaft 27 is located at 26 right back of first rotating shaft.Preferably, the second mechanical arm 12
With positional fault warning function, when positional fault occurs in 12 motion process of second mechanical arm, alarm signal is sent.
Second probe 11 is used to fill hemolysin and PBS solution.Second probe 11 has anti-collision, when described second
Probe 11 is subject to be automatically stopped when resistance or the shock of vertical direction, and sends alarm signal.
Disk module is centrifuged, as shown in fig. 7, comprises centrifugal pan 14 and the 3rd rotation being arranged on below the centrifugal pan 14 are driven
Motivation structure 15.The centrifugal pan 14 is discoid, and the centrifugal pan 14 is circumferentially equidistantly arranged with 24 reaction pipe holders 16,
Two reaction tube seats 17 are provided with each reaction pipe holder 16, a reaction is placed with each reaction tube seat 17
Pipe, is each loaded with the reaction solution that the blood sample, the antibody and the hemolysin are formed in the reaction tube.In this implementation
In example, the volume of reaction solution is 2mL in the reaction tube.The incubation reaction of the reaction solution is completed in the reaction tube.Institute
Centrifugal pan 14 is stated by the vibrations of itself and fills the liquid impulse force of hemolysin and makes the blood sample, the antibody and described molten
Sanguinin mixes, easy to operate, and cost is relatively low.3rd rotary drive mechanism 15 is suitable for driving the centrifugal pan 14 to realize
Hour hands rotate counterclockwise, and in the present embodiment, the rotating speed of the centrifugal pan 14 is 0-2500r/min.3rd rotation is driven
Motivation structure 15 is additionally operable to drive the realization of centrifugal pan 14 to be accurately positioned, so that specified reaction tube is stopped to the position specified,
To coordinate first probe 7 and second probe 11 to complete pipetting.Preferably, the 3rd rotary drive mechanism 15
Side is provided with cushioning support-saddle 25, and when the centrifugal pan 14 centrifuges clockwise or counterclockwise, the cushioning support-saddle 25 can
To reduce the vibration amplitude of the centrifugal pan 14, so as to ensure that the positioning of the centrifugal pan 14 is more accurate.The centrifugal pan 14
Outside be provided with the second temperature control cavity 30, the setting of second temperature control cavity 30 is to ensure the reaction temperature of the centrifugal pan 14
Degree maintains 37 DEG C, makes it closer to the temperature of human body.
3rd liquid relief arm module, including the 3rd probe 18, the three-mechanical arm 19 for fixing the 3rd probe 18, use
In rotating 5th driving mechanism 20 of the drive three-mechanical arm 19 and for driving the three-mechanical arm 19 to move up and down
The 6th driving mechanism 21.The three-mechanical arm 19 by the 3rd shaft 28 successively with the 5th driving mechanism 20 and described
6th driving mechanism 21 connects, and the 3rd shaft 28 is located at the right side of second shaft 27.Preferably, the 3rd machinery
Arm 19 has positional fault warning function, when positional fault occurs in 19 motion process of three-mechanical arm, sends alarm
Signal.3rd probe 18 is used to aspirate the supernatant after centrifugation.3rd probe 18 has anti-collision, when described
3rd probe 18 is subject to be automatically stopped when resistance or the shock of vertical direction, and sends alarm signal.Preferably, the described 3rd
The second liquid level sensor (not shown) is provided with mechanical arm 19, second liquid level sensor is used for described in detection automatically
The liquid level of supernatant in reaction tube, ensures that the 3rd probe 18 possesses delineation pumping function under side when sucting clear liquid,
Avoid the 3rd probe 18 and the supernatant contact too deep, cause the 3rd probe 18 in the bad cleaning of service sink.
Preferably, first driving mechanism 9, second driving mechanism 10, the 3rd driving mechanism 13, described
Four driving mechanisms, the 5th driving mechanism 20 and the 6th driving mechanism 21 are Walk stepper motors, and first rotation is driven
Motivation structure 2, second rotary drive mechanism 5 and the 3rd rotary drive mechanism 15 are servomotor.
Cleaning module, the cleaning module include cleaning pump (not shown), syringe pump (not shown), solenoid valve
(not shown), fluid path pipeline (not shown), service sink, cleaning solution bucket (not shown) and waste liquid barrel are (in figure not
Show), first probe 7, second probe 11 and the 3rd probe 18 are connected with the syringe pump respectively, can be passed through
The absorption and discharge of the antibody, the blood sample, the hemolysin and the PBS solution are completed in the control of the syringe pump,
The syringe pump is connected by the solenoid valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning solution bucket, institute
State cleaning pump discharge to be connected with the service sink by the fluid path pipeline, the service sink is connected with waste liquid barrel.
Wherein, the service sink includes the first service sink 22, the second service sink 23 and the 3rd service sink 24, and described first
Service sink 22 is arranged on the lower section of first probe 7, and second service sink 23 is arranged under second probe 11
Side, the 3rd service sink 24 are arranged on the lower section of the 3rd probe 18.First probe 7 after each will
Return first service sink 22 to clean once, second probe 11 will return second service sink clear 23 after each
Wash once, the 3rd probe 18 will return the 3rd service sink 24 and clean once after each, so as to greatest extent
Avoid cross contamination, ensure the control of cross contamination rate within 0.05%.
The separate type sample pre-treatment device further includes operation control module, and the operation control module is used to control institute
State sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module, the 3rd shifting
The action of liquid arm module and the centrifugation disk module.Specifically, the operation control module includes touch-screen 31 and control calculates
Machine, the control computer provide control operation for each module in the separate type sample pre-treatment device, whole to control
Sample process process.
One sample process process includes the following steps：Add antibody → heal sample → incubation → mixing → solubilization sanguinin →
Centrifuging → draw up clear liquid, → plus PBS solution → centrifuges → draws up that clear liquid → plus PBS solution → centrifuges → draw up clear liquid → plus PBS is molten
Liquid.
Preferably, the sample disk module, the antibody disk module, the first liquid relief arm module, second liquid relief
Arm module, the 3rd liquid relief arm module and the centrifugation disk module are integrated on an instrument host 32, the touch-screen 31
It is arranged in the cabinet of the instrument host 32.
Preferably, rail plate (not shown), the touch-screen 31 are provided with the cabinet of the instrument host 32
It is connected with the rail plate, the touch-screen 31 is extracted out or is pushed into the cabinet of the instrument host 32, it is convenient to carry out
Operation.
Embodiment two
A kind of separate type sample pre-treatment device is present embodiments provided, which is suitable for the processing of whole blood sample.
As shown in Figures 1 to 4, separate type sample pre-treatment device provided in this embodiment includes：
Sample disk module, as shown in figure 5, including sample disk 1 and the first rotation driving being arranged on below the sample disk 1
Mechanism 2, first rotary drive mechanism 2 are used to drive the sample disk 1 to rotate.Preferably, the sample disk 1 is disk
Shape, the sample disk 1 are circumferentially equidistantly arranged with 25 sample tube seats 3, and one is placed with each sample tube seat 3
Sample tube, is each loaded with blood sample described in 50-150 μ L in the sample tube.
Antibody disk module, as shown in fig. 6, including antibody disk 4 and the second rotation driving being arranged on below the antibody disk 4
Mechanism 5, second rotary drive mechanism 5 are used to drive the antibody disk 4 to rotate.Preferably, the antibody disk 4 is disk
Shape, the antibody disk 4 are circumferentially equidistantly arranged with 25 antibody tube seats 6, and one is placed with each antibody tube seat 6
Antibody pipe, is each loaded with 5-25 μ L antibody in the antibody pipe.Preferably, the outside of the antibody disk 4 is provided with the first temperature
Chamber 29 is controlled, first temperature control cavity 29 is used to make the temperature of the antibody disk 4 maintain 2~8 DEG C, so as to extend the antibody
Service life.
First liquid relief arm module, including the first probe 7, the first mechanical arm 8 for fixing first probe 7, be used for
Drive rotating first driving mechanism 9 of the first mechanical arm 8 and second for driving that the first mechanical arm 8 moves up and down
Driving mechanism 10.The first mechanical arm 8 is driven with first driving mechanism 9 and described second successively by first rotating shaft 26
Mechanism 10 connects, and the first rotating shaft 26 is located at the right side of the sample disk 1 and the antibody disk 4, and the first rotating shaft 26
The distance between described 1 center of sample disk and the first rotating shaft 26 are equal with the distance between 4 center of antibody disk.It is excellent
Choosing, the first mechanical arm 8 has positional fault warning function, when position occurs in 8 motion process of first mechanical arm
During mistake, alarm signal is sent.First probe 7 is applied not only to draw the antibody and the blood sample, is additionally operable to quantitative
Fill the antibody and the blood sample.First probe 7 has anti-collision, when first probe 7 is subject to vertically
It can be automatically stopped when the resistance in direction or shock, and send alarm signal.It is worth noting that, the internal diameter of first probe 7
Should not it is too big also should not be too small, if excessive, the precision of antibody described in compressing can be caused to be not allowed；If too small, can cause described in compressing
Less cleaning position after blood sample and stifled pin.In order to avoid the antibody wastes, the first liquid level is provided with the first mechanical arm 8
Sensor (not shown), for detecting the liquid level of the antibody in the antibody pipe automatically, ensures that the antibody is maximum
Degree blots.
Second liquid relief arm module, including the second probe 11, the second mechanical arm 12 for fixing second probe 11, use
In rotating 3rd driving mechanism 13 of the drive second mechanical arm 12 and for driving the second mechanical arm 12 to move up and down
The 4th driving mechanism.The second mechanical arm 12 by the second shaft 27 successively with the 3rd driving mechanism 13 and described
Four driving mechanisms connect, and second shaft 27 is located at 26 right back of first rotating shaft.Preferably, the second mechanical arm 12
With positional fault warning function, when positional fault occurs in 12 motion process of second mechanical arm, alarm signal is sent.
Second probe 11 is used to fill hemolysin and PBS solution.Second probe 11 has anti-collision, when described second
Probe 11 is subject to be automatically stopped when resistance or the shock of vertical direction, and sends alarm signal.
Disk module is centrifuged, as shown in fig. 7, comprises centrifugal pan 14 and the 3rd rotation being arranged on below the centrifugal pan 14 are driven
Motivation structure 15.The centrifugal pan 14 is discoid, and the centrifugal pan 14 is circumferentially equidistantly arranged with 24 reaction pipe holders 16,
Two reaction tube seats 17 are provided with each reaction pipe holder 16, a reaction is placed with each reaction tube seat 17
Pipe, is each loaded with the reaction solution that the blood sample, the antibody and the hemolysin are formed in the reaction tube.In this implementation
In example, the volume of reaction solution is 2mL in the reaction tube.The incubation reaction of the reaction solution is completed in the reaction tube.Institute
Centrifugal pan 14 is stated by the vibrations of itself and fills the liquid impulse force of hemolysin and makes the blood sample, the antibody and described molten
Sanguinin mixes, easy to operate, and cost is relatively low.3rd rotary drive mechanism 15 is suitable for driving the centrifugal pan 14 to realize
Hour hands rotate counterclockwise, and in the present embodiment, the rotating speed of the centrifugal pan 14 is 0-2500r/min.3rd rotation is driven
Motivation structure 15 is additionally operable to drive the realization of centrifugal pan 14 to be accurately positioned, so that specified reaction tube is stopped to the position specified,
To coordinate first probe 7 and second probe 11 to complete pipetting.Preferably, the 3rd rotary drive mechanism 15
Side is provided with cushioning support-saddle 25, and when the centrifugal pan 14 centrifuges clockwise or counterclockwise, the cushioning support-saddle 25 can
To reduce the vibration amplitude of the centrifugal pan 14, so as to ensure that the positioning of the centrifugal pan 14 is more accurate.The centrifugal pan 14
Outside be provided with the second temperature control cavity 30, the setting of second temperature control cavity 30 is to ensure the reaction temperature of the centrifugal pan 14
Degree maintains 37 DEG C, makes it closer to the temperature of human body.
3rd liquid relief arm module, including the 3rd probe 18, the three-mechanical arm 19 for fixing the 3rd probe 18, use
In rotating 5th driving mechanism 20 of the drive three-mechanical arm 19 and for driving the three-mechanical arm 19 to move up and down
The 6th driving mechanism 21.The three-mechanical arm 19 by the 3rd shaft 28 successively with the 5th driving mechanism 20 and described
6th driving mechanism 21 connects, and the 3rd shaft 28 is located at the right side of second shaft 27.Preferably, the 3rd machinery
Arm 19 has positional fault warning function, when positional fault occurs in 19 motion process of three-mechanical arm, sends alarm
Signal.3rd probe 18 is used to aspirate the supernatant after centrifugation.3rd probe 18 has anti-collision, when described
3rd probe 18 is subject to be automatically stopped when resistance or the shock of vertical direction, and sends alarm signal.Preferably, the described 3rd
The second liquid level sensor (not shown) is provided with mechanical arm 19, second liquid level sensor is used for described in detection automatically
The liquid level of supernatant in reaction tube, ensures that the 3rd probe 18 possesses delineation pumping function under side when sucting clear liquid,
Avoid the 3rd probe 18 and the supernatant contact too deep, cause the 3rd probe 18 in the bad cleaning of service sink.
Preferably, first driving mechanism 9, second driving mechanism 10, the 3rd driving mechanism 13, described
Four driving mechanisms, the 5th driving mechanism 20 and the 6th driving mechanism 21 are Walk stepper motors, and first rotation is driven
Motivation structure 2, second rotary drive mechanism 5 and the 3rd rotary drive mechanism 15 are servomotor.
Cleaning module, the cleaning module include cleaning pump (not shown), syringe pump (not shown), solenoid valve
(not shown), fluid path pipeline (not shown), service sink, cleaning solution bucket (not shown) and waste liquid barrel are (in figure not
Show), first probe 7, second probe 11 and the 3rd probe 18 are connected with the syringe pump respectively, can be passed through
The absorption and discharge of the antibody, the blood sample, the hemolysin and the PBS solution are completed in the control of the syringe pump,
The syringe pump is connected by the solenoid valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning solution bucket, institute
State cleaning pump discharge to be connected with the service sink by the fluid path pipeline, the service sink is connected with waste liquid barrel.
Wherein, the service sink includes the first service sink 22, the second service sink 23 and the 3rd service sink 24, and described first
Service sink 22 is arranged on the lower section of first probe 7, and second service sink 23 is arranged under second probe 11
Side, the 3rd service sink 24 are arranged on the lower section of the 3rd probe 18.First probe 7 after each will
Return first service sink 22 to clean once, second probe 11 will return second service sink clear 23 after each
Wash once, the 3rd probe 18 will return the 3rd service sink 24 and clean once after each, so as to greatest extent
Avoid cross contamination, ensure the control of cross contamination rate within 0.05%.
The separate type sample pre-treatment device further includes operation control module, and the operation control module is used to control institute
State sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module, the 3rd shifting
The action of liquid arm module and the centrifugation disk module.Specifically, the operation control module includes touch-screen 31 and control calculates
Machine, the control computer provide control operation for each module in the separate type sample pre-treatment device, whole to control
Sample process process.
One sample process process includes the following steps：Add antibody → heal sample → incubation → mixing → solubilization sanguinin →
Centrifuging → draw up clear liquid, → plus PBS solution → centrifuges → draws up that clear liquid → plus PBS solution → centrifuges → draw up clear liquid → plus PBS is molten
Liquid.
Preferably, the sample disk module, the antibody disk module, the first liquid relief arm module, second liquid relief
Arm module, the 3rd liquid relief arm module and the centrifugation disk module are integrated on an instrument host 32, the touch-screen 31
It is arranged in the cabinet of the instrument host 32.
Preferably, rail plate (not shown), the touch-screen 31 are provided with the cabinet of the instrument host 32
It is connected with the rail plate, the touch-screen 31 is extracted out or is pushed into the cabinet of the instrument host 32, it is convenient to carry out
Operation.
The separate type sample pre-treatment device further includes scan sample module (not shown), the scan sample mould
Block is arranged on the outside of the sample tube, and is connected with the operation control module, can be put into by scanning on the outside of the sample tube
Quick Response Code, feed back to the operation control module, the experiment flow of the sample tube controlled by the operation control module.
Separate type sample pre-treatment device provided by the utility model, can be good at overcoming artificial treatment to bring lacks
Point, completes the cell preparation work of blood sample before machine analysis on flow cytometer, can largely save manually but also improve blood
Sample process effect.
The above is only the preferred embodiment of the utility model, general based on the embodiment in the utility model, this area
Logical technical staff all other embodiments obtained without making creative work, it is new should all to belong to this practicality
The scope of type protection.It should be pointed out that for those skilled in the art, the utility model principle is not being departed from
On the premise of, some improvements and modifications can also be made, these improvements and modifications also should be regarded as the scope of protection of the utility model.
Claims (9)
- A kind of 1. separate type sample pre-treatment device, it is characterised in that including：Sample disk module, including sample disk (1) and for driving rotating first rotary drive mechanism (2) of the sample disk (1), The sample disk (1) is circumferentially equidistantly arranged with multiple sample tube seats (3)；Antibody disk module, including antibody disk (4) and for driving rotating second rotary drive mechanism (5) of the antibody disk (4), The antibody disk (4) is circumferentially equidistantly arranged with multiple antibody tube seats (6)；First liquid relief arm module, including the first probe (7), the first mechanical arm (8) for fixing first probe (7), use In rotating first driving mechanism (9) of the drive first mechanical arm (8) and for driving the first mechanical arm (8) to transport up and down Dynamic the second driving mechanism (10), first probe (7) are used to draw and fill antibody and sample；Second liquid relief arm module, including the second probe (11), the second mechanical arm (12) for fixing second probe (11), For driving rotating 3rd driving mechanism (13) of the second mechanical arm (12) and for driving the second mechanical arm (12) The 4th driving mechanism to move up and down, second probe (11) are used to fill lysate and buffer solution；Centrifuge disk module, including centrifugal pan (14) and for driving rotating 3rd rotary drive mechanism of the centrifugal pan (14) (15), the centrifugal pan (14) is circumferentially equidistantly arranged with multiple reaction pipe holders (16), each reaction pipe holder (16) it is provided with least one reaction tube seat (17) on, the centrifugal pan (14) is used for realization the sample, antibody, described Incubation, mixing and the centrifugation of lysate and the buffer solution, the 3rd rotary drive mechanism (15) are used to drive the centrifugation Disk (14) is realized and rotates clockwise or counterclockwise and be accurately positioned；3rd liquid relief arm module, including the 3rd probe (18), the three-mechanical arm (19) for fixing the 3rd probe (18), For driving rotating 5th driving mechanism (20) of the three-mechanical arm (19) and for driving the three-mechanical arm (19) The 6th driving mechanism (21) to move up and down, the 3rd probe (18) are used to aspirate the supernatant after centrifugation.
- 2. separate type sample pre-treatment device according to claim 1, it is characterised in that the separate type Sample pretreatment Device further includes cleaning module, and the cleaning module includes cleaning pump, syringe pump, solenoid valve, fluid path pipeline, service sink and cleaning Liquid bucket, first probe (7), second probe (11) and the 3rd probe (18) are connected with the syringe pump respectively, The syringe pump is connected by the solenoid valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning solution bucket, institute Cleaning pump discharge is stated to be connected with the service sink by the fluid path pipeline.
- 3. separate type sample pre-treatment device according to claim 2, it is characterised in that it is clear that the service sink includes first Wash pool (22), the second service sink (23) and the 3rd service sink (24), first service sink (22) are arranged on first probe (7) lower section, second service sink (23) are arranged on below second probe (11), and the 3rd service sink (24) is arranged on Below 3rd probe (18).
- 4. separate type sample pre-treatment device according to claim 1, it is characterised in that the centrifugal pan (14) is by certainly The vibrations of body and the liquid impulse force of the filling lysate mix the sample, the antibody and the lysate.
- 5. separate type sample pre-treatment device according to claim 1, it is characterised in that the 3rd rotary drive mechanism (15) side is provided with the cushioning support-saddle (25) for reducing the centrifugal pan (14) vibration amplitude.
- 6. separate type sample pre-treatment device according to claim 1, it is characterised in that the first mechanical arm (8) is logical Cross first rotating shaft (26) to be connected with first driving mechanism (9) and second driving mechanism (10) successively, described first turn Between the distance between axis (26) and the sample disk (1) center and the first rotating shaft (26) and the antibody disk (4) center Distance it is equal.
- 7. separate type sample pre-treatment device according to claim 1, it is characterised in that on the first mechanical arm (8) The first liquid level sensor is provided with, the liquid level that first liquid level sensor is used to detect antibody in the antibody pipe automatically is high Spend, the second liquid level sensor is provided with the three-mechanical arm (19), second liquid level sensor is used to detect institute automatically State the liquid level of supernatant in reaction tube.
- 8. separate type sample pre-treatment device according to claim 1, it is characterised in that the outside of the antibody disk (4) The first temperature control cavity (29) is provided with, first temperature control cavity (29) is used to make the temperature of the antibody disk (4) maintain 2~8 DEG C； The outside of the centrifugal pan (14) is provided with the second temperature control cavity (30), and second temperature control cavity (30) is used to make the centrifugal pan (14) reaction temperature maintains 37 DEG C.
- 9. separate type sample pre-treatment device according to claim 1, it is characterised in that pair of the sample tube seat (3) Answer and sample tube is placed with position, the sample tube is used to contain the sample；Put on the correspondence position of the antibody tube seat (6) Antibody pipe is equipped with, the antibody pipe is used to contain the antibody；Reaction is placed with the correspondence position of the reaction tube seat (17) Manage, the reaction solution that the sample, the antibody and the lysate are formed is loaded with the reaction tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721008009.2U CN207366314U (en) | 2017-08-14 | 2017-08-14 | A kind of separate type sample pre-treatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721008009.2U CN207366314U (en) | 2017-08-14 | 2017-08-14 | A kind of separate type sample pre-treatment device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207366314U true CN207366314U (en) | 2018-05-15 |
Family
ID=62422023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721008009.2U Active CN207366314U (en) | 2017-08-14 | 2017-08-14 | A kind of separate type sample pre-treatment device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207366314U (en) |
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2017
- 2017-08-14 CN CN201721008009.2U patent/CN207366314U/en active Active
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Effective date of registration: 20231228 Address after: 230000, West Half Floor, 2nd Floor, Building 2, Phase II, Intelligent Science and Technology Park, No. 3959 Susong Road, Hefei Economic and Technological Development Zone, Anhui Province Patentee after: Zhongsheng Medical Technology (Hefei) Co.,Ltd. Address before: Room 101, 3rd Floor, Building 3, No. 8 Jinfeng Road, Suzhou High tech Zone, Suzhou City, Jiangsu Province, 215000 Patentee before: ZHONGSHENG (SUZHOU) MEDICAL TECHNOLOGY CO.,LTD. |
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