CN207266856U - A kind of purifying centrifuge tube with seperation film - Google Patents
A kind of purifying centrifuge tube with seperation film Download PDFInfo
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- CN207266856U CN207266856U CN201721163213.1U CN201721163213U CN207266856U CN 207266856 U CN207266856 U CN 207266856U CN 201721163213 U CN201721163213 U CN 201721163213U CN 207266856 U CN207266856 U CN 207266856U
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- centrifuge tube
- seperation film
- purifying
- centrifugation
- pipe shaft
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Abstract
It the utility model is related to a kind of purifying centrifuge tube with seperation film.The purpose is to provide a kind of purifying centrifuge tube with seperation film, there is simple in structure, easy to operate, good effect of separating solid from liquid, the cost of protein purification can be significantly reduced.A kind of purifying centrifuge tube with seperation film, including：Centrifuge pipe shaft and centrifugation tube cover, the centrifugation pipe shaft and centrifugation tube cover realize sealed connection using the repeatable structure opened, the purification filler of purifying protein is useful in the underfill of the centrifugation pipe shaft, in the top of the purification filler, equipped with one layer of seperation film, the seperation film is fixed at the close position of bottleneck of the centrifugation pipe shaft, its filter sizes size can be by can not be by being advisable and purification filler with solution and target protein.
Description
Technical field
Protein separation technology field is the utility model is related to, is to be related to a kind of band seperation film specifically
Purifying centrifuge tube.
Background technology
Isolating and purifying for protein is the technology being commonly used in the exploitation of macromolecular drug, when the sample that needs purify
Product quantity is many and during small volume, purification filler is directly added into sample centrifuge tube by generally use, by absorption, washing,
The albumen of purifying is afforded, uses protein purification instrument purifying protein compared to conventional, the flux of this method is higher.At this
In kind way of purification, there is the operation of multistep separation of solid and liquid, if using conventional purification filler, need to be realized by the way of centrifugation
Separation of solid and liquid, but ordinary gel filler is not easy to form firm precipitation in centrifugal process, influences separation of solid and liquid, therefore usually need
The expensive purification filler with magnetic bead is used, magnetic frame is arranged in pairs or groups to realize separation of solid and liquid, so can significantly increase purifying
Cost.
Utility model content
The purpose of the utility model is to overcome shortcoming of the prior art, there is provided a kind of purifying with seperation film with from
Heart pipe, has the characteristics that simple in structure, easy to operate, good effect of separating solid from liquid, can significantly reduce the cost of protein purification.
What the purpose of this utility model was realized in：
A kind of purifying centrifuge tube with seperation film, including：Centrifuge pipe shaft and centrifugation tube cover, the centrifugation pipe shaft and centrifugation
Tube cover is realized using the repeatable structure opened and is tightly connected, and purifying protein is useful in the underfill of the centrifugation pipe shaft
Purification filler, in the top of the purification filler, equipped with one layer of seperation film, the seperation film is fixed on leaning on for the centrifugation pipe shaft
At nearly position of bottleneck, its filter sizes size can be by can not be by being advisable and purification filler with solution and target protein.
The utility model in purifying centrifuge tube by being previously added a certain amount of purification filler, and in conventional centrifugal pipe
On the basis of one separation membrane structure of increase, the aperture of seperation film can allow solution and protein by but retaining purification filler.
This structure make it that conventional gel filler can be used in operation in isolating and purifying for protein, even if there is the part solidifying after centrifugation
Glue filler does not form firm precipitation, can be retained in separation of solid and liquid process by seperation film yet, not interfere with separation of solid and liquid
Effect, can significantly reduce the cost of protein purification.
Wherein, cut body on the centrifuge tube and the lower section body of centrifuge tube is threadedly tightly connected.
Purifying centrifuge tube with seperation film as described above, because centrifuge tube is as integrative-structure, according to the pure of prepackage
Change the type of filler, the purification work of the specific protein to match suitable for the purification filler with the prepackage.
As the another embodiment of the utility model, a kind of purifying centrifuge tube with seperation film, including：Centrifuge pipe shaft
With centrifugation tube cover, the centrifugation pipe shaft and centrifugation tube cover realize sealed connection, the centrifuge tube using the repeatable structure opened
Body is made of on centrifuge tube sections of body and the lower section body of centrifuge tube, and the underfill of section body is useful for pure under the centrifuge tube
Change the purification filler of albumen, in the top of the purification filler, equipped with one layer of seperation film, the seperation film is fixed on the centrifugation
Cut on pipe at the close position of bottleneck of body, solution and protein can cannot pass through institute by the seperation film, purification filler
State seperation film.
Wherein, cut body on the centrifuge tube and the lower section body of centrifuge tube is threadedly tightly connected；The centrifugation
Pipe shaft and centrifugation tube cover are threadably tightly connected.
Purifying centrifuge tube with seperation film as described above, can reuse, and since centrifugation pipe shaft is by separating
Centrifuge tube on cut body and centrifuge tube lower section body composition, the purification filler of the underfill of lower section of body of centrifuge tube can root
It is different according to needing to select, coordinate and be fixed with cutting body one on the centrifuge tube of the matched seperation film of selected purification filler
Rise and use, realize protein purification.Its operating method is identical with aforementioned embodiments, but can be adapted for different types of albumen
Purification work.
Brief description of the drawings
By the embodiment of following the utility model and description taken together with the accompanying drawings, show the utility model further advantage and
Feature, which provides by way of example, but is not limited to this, wherein：
Fig. 1 is a kind of structural representation of a preferred embodiment of the purifying centrifuge tube with seperation film of the utility model
Figure.
Fig. 2 is a kind of structural representation of another preferred embodiment of the purifying centrifuge tube with seperation film of the utility model
Figure.
In figure：1st, centrifuge tube pipe shaft；11st, body is cut on centrifuge tube；12nd, lower section of body of centrifuge tube；2nd, tube cover is centrifuged；3rd, divide
From film；4th, purification filler.
Embodiment
Purifying centrifuge tube with seperation film as shown in Figure 1, including：Pipe shaft 1 and centrifugation tube cover 2 are centrifuged, centrifuges pipe shaft 1
Threadably it is tightly connected with centrifugation tube cover 2, has a certain amount of purifying for purifying protein in the underfill of centrifugation pipe shaft 1
Filler 4, in the top of purification filler 4, equipped with one layer of seperation film 3.Seperation film 3 is fixed on the close position of bottleneck of centrifugation pipe shaft 1
Place.
Wherein, the selection criteria of purification filler 4 is：The target egg is adapted to according to the different selection of the protein types to be purified
White purification filler.The filter sizes size of seperation film 3 be with target protein can by and purification filler can not be by being advisable.
In use, protein solution to be purified is added into being preinstalled with the centrifuge tube of purification filler 4 in above-described embodiment,
Concussion is so that purification filler 4 is fully mixed with protein solution, and concussion is incubated 4-12h on shaking table, and 1500g centrifugation 10min, are abandoned
Remove supernatant；Cleaning solution is added, concussion is so that purification filler is fully mixed with protein solution, and concussion is incubated 30min on shaking table,
1500g centrifuges 10min, supernatant discarding；Suitable eluent is added, concussion causes purification filler fully to be mixed with protein solution,
And concussion is incubated 30min on shaking table, 1500g centrifugation 10min, collect supernatant, the as solution containing purifying protein.
The purifying centrifuge tube with seperation film described in the present embodiment, can reuse, because centrifugation pipe shaft 1 is
Integrative-structure, according to the type of the purification filler of prepackage, the specific protein to match suitable for the purification filler with the prepackage
Purification work.
Purifying centrifuge tube with seperation film as shown in Figure 2, including：Pipe shaft 1 and centrifugation tube cover 2 are centrifuged, centrifuges pipe shaft 1
Threadably it is tightly connected with centrifugation tube cover 2, has a certain amount of purifying for purifying protein in the underfill of centrifugation pipe shaft 1
Filler 4, in the top of purification filler 4, equipped with one layer of seperation film 3.Seperation film 3 is fixed on the close position of bottleneck of centrifugation pipe shaft 1
Place.
Wherein, centrifuge pipe shaft 1 to be formed by cutting lower section of body 12 of body 11 and centrifuge tube on centrifuge tube, cut on centrifuge tube
Lower section of body 12 of body 11 and centrifuge tube is threadedly tightly connected, and seperation film 3, which is arranged on centrifuge tube, cuts body 11
At position of bottleneck, purification filler 4 is filled in the bottom of lower section of body 12 of centrifuge tube.
The selection criteria of purification filler 4 is：The target protein is adapted to according to the different selection of the protein types to be purified
Purification filler.The filter sizes size of seperation film 3 be with target protein can by and purification filler can not be by being advisable.
The purifying centrifuge tube with seperation film described in the present embodiment, can reuse, and due to centrifuging pipe shaft 1
Formed by cutting lower section of body 12 of body 11 and centrifuge tube on separable centrifuge tube, the underfill of lower section of body 12 of centrifuge tube
Purification filler 4 can select different as needed, coordinate and be fixed with and the matched seperation film 3 of selected purification filler 4
Centrifuge tube on cut a body 11 be used together, realize protein purification.Its operating method is identical with a upper embodiment, but can be applicable in
In the purification work of different types of albumen.
Although the utility model is hereinbefore illustrated according to preferred embodiment, it is not intended that the utility model
Scope be confined to above-mentioned structure, as long as those skilled in the art can be easily after above-mentioned explanation is read
The equivalent substitution structure developed, the equivalent changes and modifications made under the spirit and scope for not departing from the utility model, all
It should be covered by within the scope of the utility model patent.
Claims (5)
- A kind of 1. purifying centrifuge tube with seperation film, it is characterised in that including：Centrifuge pipe shaft and centrifugation tube cover, the centrifugation Pipe shaft and centrifugation tube cover are realized using the repeatable structure opened to be tightly connected, and is useful in the underfill of the centrifugation pipe shaft The purification filler of purifying protein, in the top of the purification filler, equipped with one layer of seperation film, the seperation film be fixed on it is described from At the close position of bottleneck of heart pipe shaft, solution and protein can cannot pass through described point by the seperation film, purification filler From film.
- 2. the purifying centrifuge tube with seperation film as claimed in claim 1, it is characterised in that：The centrifugation pipe shaft is by centrifuge tube The upper lower section of body of body and centrifuge tube that cut is formed, and the seperation film is arranged on the close position of bottleneck that body is cut on the centrifuge tube Place, the purification filler are filled in the bottom of lower section of body of the centrifuge tube.
- 3. the purifying centrifuge tube with seperation film as claimed in claim 2, it is characterised in that：On the centrifuge tube cut body and The lower section body of centrifuge tube is threadedly tightly connected.
- 4. the purifying centrifuge tube with seperation film as described in any claim in claim 1-3, it is characterised in that：It is described Centrifugation pipe shaft and centrifugation tube cover are threadably tightly connected.
- 5. the purifying centrifuge tube with seperation film as described in any claim in claim 1-3, it is characterised in that：It is described Purification filler is gel filler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721163213.1U CN207266856U (en) | 2017-09-12 | 2017-09-12 | A kind of purifying centrifuge tube with seperation film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721163213.1U CN207266856U (en) | 2017-09-12 | 2017-09-12 | A kind of purifying centrifuge tube with seperation film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207266856U true CN207266856U (en) | 2018-04-24 |
Family
ID=61960658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721163213.1U Active CN207266856U (en) | 2017-09-12 | 2017-09-12 | A kind of purifying centrifuge tube with seperation film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207266856U (en) |
-
2017
- 2017-09-12 CN CN201721163213.1U patent/CN207266856U/en active Active
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