CN207240299U - A kind of automation equipment for tissue block cutting - Google Patents
A kind of automation equipment for tissue block cutting Download PDFInfo
- Publication number
- CN207240299U CN207240299U CN201721184805.1U CN201721184805U CN207240299U CN 207240299 U CN207240299 U CN 207240299U CN 201721184805 U CN201721184805 U CN 201721184805U CN 207240299 U CN207240299 U CN 207240299U
- Authority
- CN
- China
- Prior art keywords
- cutting
- knife
- tissue block
- housing
- automation equipment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
It the utility model is related to experiment equipment technical field, more particularly to a kind of automation equipment for tissue block cutting.Automation equipment provided by the utility model for tissue block cutting, including housing and the cutting mechanism in housing；Cutting mechanism includes knife group and drives the actuating unit that movable cutter set rotates, knife group includes the cutter shaft of two relative rotations, the set of blades being vertically arranged is equipped with every cutter shaft, and the roller knife blade of the set of blades on two cutter shafts is in interlaced setting, forms the cutting channel for being used for cutting tissue block；The upper end of housing is equipped with feed inlet, and feed inlet is located at the top of the input end of cutting channel, and the front end of housing is equipped with the discharge port connected with the port of export of cutting channel.The device for being used to cut tissue block that the application provides, simple in structure, design is reasonable；Required tissue block is obtained by the way of automation, time saving and energy saving, production efficiency is high；It is highly practical and the tissue block size obtained is homogeneous, effectively increases the quality of production.
Description
Technical field
It the utility model is related to experiment equipment technical field, more particularly to a kind of automatic makeup for tissue block cutting
Put.
Background technology
At present, umbilical cord mesenchymal stem cells are extracted from umbilical cord China Tong Shi glue and mainly uses China Tong Shi glue tissue block adherent methods
Prepared, it is then the committed step for extracting mescenchymal stem cell to handle and shred China's Tong Shi glue.
In actual mechanical process, tissue adherent method needs umbilical cord China Tong Shi glue tissue shears being broken into small tissue blocks, is easy to
Tissue block is attached to bottom of culture vessel.Cut tissue block size is suitable, and tissue block is too big, is unfavorable for mescenchymal stem cell trip
Tissue block is separated out, tissue block is too small, then adds culture medium easily to float again after tissue block adherent.As patent CN102127522B,
CN105586309A, CN101914496B are required to shred tissue block to 1mm3~3mm3Size.
Magnificent Tong Shi glue tissue block is usually placed in culture dish by current techniques personnel, using eye scissors by the way of blind cut
Umbilical cord China Tong Shi glue tissues are shredded, operability is poor, as a result causes the magnificent Tong Shi glue tissue block of acquisition not of uniform size, influences very much
The quality and quantity that mescenchymal stem cell is separated out from magnificent Tong Shi glue middle reaches during follow-up cultivation.In addition, shred a umbilical cord China
The time of Tong Shi glue, time and effort consuming, handled more umbilical cord tissues, this causes work with greater need for interior in short-term sometimes at 30~60 minutes
Have a greatly reduced quality as efficiency.
Utility model content
(1) technical problems to be solved
The purpose of this utility model is：There is provided a kind of simple in structure, design rationally and can ensure the same of tissue block quality
When effectively improve cutting efficiency be used for tissue block cutting automation equipment, by solve using it is blind cut in a manner of shred umbilical cord China
Tissue block existing for Tong Shi glue tissues is not of uniform size, time and effort consuming and the problem of inefficiency.
(2) technical solution
In order to solve the above-mentioned technical problem, the utility model provides a kind of automation equipment for tissue block cutting,
Cutting mechanism including housing and in the housing；The cutting mechanism includes knife group and drives the dynamic of the knife group rotation
Force mechanisms, the knife group include the cutter shaft of two relative rotations, the set of blades being vertically arranged are equipped with the every cutter shaft, and
The roller knife blade of set of blades on two cutter shafts is in interlaced setting, forms the cutting channel for being used for cutting tissue block；
The upper end of the housing is equipped with feed inlet, and the feed inlet is located at the top of the input end of the cutting channel, the housing
Front end is equipped with the discharge port connected with the port of export of the cutting channel.
Preferably, the knife group further includes two groups of knife combs, knife comb described in two groups and two blades being arranged on the cutter shaft
Group, which corresponds, to be set；There is empty slot between the roller knife blade of the set of blades, the knife comb being correspondingly arranged is inserted into the roller knife
In empty slot between blade；The installation end of the knife comb is fixed on the upper end of the housing, and the broach of the knife comb extends downwardly
To the bottom of the set of blades.
Preferably, the tablet being obliquely installed is additionally provided with the housing, the tablet is located at the lower section of knife comb, and institute
The one end for stating tablet is connected with the cutting channel, and the other end is connected with the discharge port, is used to convey the tissue to form
The transfer passage of block.
Preferably, wherein one end of a cutter shaft is connected with the actuating unit, the other end is equipped with main linkage grinding tooth
Wheel；The end of another cutter shaft is equipped with the duplicate invoice being meshed with the main linkage grinding tooth wheel and moves grinding tooth wheel.
Preferably, the actuating unit includes motor and transmission component, and the transmission component includes main chain travelling gear
And the secondary chain transmission gear being connected by driving chain with the main chain travelling gear, the main chain travelling gear with it is described electronic
The driving axis connection of machine, the pair chain transmission gear are connected with the cutter shaft equipped with the main linkage grinding tooth wheel.
Preferably, the end of two cutter shafts by rotate pin respectively with the main linkage grinding tooth wheel and pair
Link the connection of grinding tooth wheel.
Preferably, the first support plate and the first pressing plate in first support plate, institute are additionally provided with the housing
The end for stating the first support plate and the first pressing plate offers groove portion, and first support plate and the groove portion of the first pressing plate are common
The slide opening for passing through and rotating for the rotation pin is limited, first pressing plate passes through fastener and the described first support
Plate is fixedly connected.
Preferably, the quantity of the knife group is two or more, axis of multiple knife groups along the cutter shaft
Direction is set gradually, and the cutter shaft of adjacent two knife groups is connected by connecting pins.
Preferably, the second support plate and the second pressing plate in second support plate, institute are additionally provided with the housing
The end for stating the second support plate and the second pressing plate offers groove portion, and second support plate and the groove portion of the second pressing plate are common
The through hole for passing through and rotating for the connecting pins is limited, second pressing plate passes through fastener and the described second support
Plate is fixedly connected.
Preferably, the quantity of the feed inlet, discharge port is consistent with the quantity of the knife group, and the feed inlet, discharging
Mouth is corresponded with the cutting channel and set.
(3) beneficial effect
The above-mentioned technical proposal of the utility model has the following advantages that：
The utility model provides a kind of automation equipment for tissue block cutting, including housing and in housing
Cutting mechanism；Cutting mechanism includes knife group and drives the actuating unit that movable cutter set rotates, and knife group includes the cutter shaft of two relative rotations,
The set of blades being vertically arranged is equipped with every cutter shaft, and the roller knife blade of the set of blades on two cutter shafts is set in interlaced
Put, form the cutting channel for being used for cutting tissue block；The upper end of housing is equipped with feed inlet, and feed inlet is located at the import of cutting channel
The top at end, the front end of housing are equipped with the discharge port connected with the port of export of cutting channel.What the application provided is used to cut group
The device of block is knitted, simple in structure, design is reasonable；In use, two cutter shafts are driven to relatively rotate by actuating unit, it is only necessary to will treat
The tissue of cutting is put into feed inlet, and tissue to be cut is cut via the cutting channel that two set of blades are formed, finally from
Discharge port skids off.In this way, obtaining required tissue block by the way of automation, cutting speed is fast, time saving and energy saving, production efficiency
It is high；And the tissue block that cutting obtains is uniform in size, consistent, effectively increases the quality of production, easy to follow-up tissue block adherent and training
Operation is supported, it is highly practical, beneficial to being standardized production and popularization.
Brief description of the drawings
Fig. 1 is the outside of automation equipment in a kind of automation equipment embodiment for tissue block cutting of the utility model
Structure diagram；
Fig. 2 be the utility model it is a kind of for tissue block cutting automation equipment embodiment in knife group, actuating unit and
The structure diagram of the connection mode of discharge port；
The company of knife group and actuating unit in a kind of automation equipment embodiment for tissue block cutting of Fig. 3 the utility model
Connect the structure diagram of mode；
Fig. 4 is knife group and the connection of knife comb in a kind of automation equipment embodiment for tissue block cutting of the utility model
The structure diagram of mode；
Fig. 5 is that the structure of actuating unit in a kind of automation equipment embodiment for tissue block cutting of the utility model is shown
It is intended to；
Fig. 6 is the structural representation that knife is combed in a kind of automation equipment embodiment for tissue block cutting of the utility model
Figure.
In figure：1：Motor；2：Main chain travelling gear；3：Driving chain；4：Secondary chain transmission gear；5：Main linkage grinding tooth
Wheel；6：Duplicate invoice moves grinding tooth wheel；7、10：Drive roll knife；8、11、13、16：Rotate pin；9、14：Connecting pins；12、15：It is driven
Roller knife；17：Second support plate；18：Second pressing plate；19、22：Fixing nut；20：First support plate；21：First pressing plate；23、
24、25、26：Knife is combed；27、28：Tablet；A1、B1：Feed inlet；A：First knife group；B：Second knife group.
Embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the part of the embodiment of the utility model, instead of all the embodiments.Based on the reality in the utility model
Apply example, those of ordinary skill in the art's all other embodiments obtained on the premise of creative work is not made, all
Belong to the scope of the utility model protection.
It should be noted that the automation equipment for being used for tissue block cutting that the application provides, available for handling and shred navel
Band China Tong Shi glue, it can also be used to the cutting process of other biological tissues.For ease of statement, the application is preferably to handle and shred navel
Exemplified by band China Tong Shi glue, to illustrate.
As shown in Figures 1 to 6, the utility model embodiment provides a kind of automation equipment for tissue block cutting,
Cutting mechanism including housing and in housing；Specifically, cutting mechanism includes knife group and drives the engine that movable cutter set rotates
Structure, knife group include the cutter shaft of two relative rotations, the set of blades being vertically arranged are equipped with every cutter shaft, and on two cutter shafts
The roller knife blade of set of blades is in interlaced setting, i.e. being embedded in corresponding blade to the roller knife blade clearance of rolling in every group of knife group
In gap, and then the cutting channel for being used for cutting tissue block is formed, wherein, the roller knife blade on two cutter shafts is equal with fixed interval (FI)
Even setting so that the tissue block size cut out is homogeneous, can high quality prepare umbilical cord mesenchymal stem cells；The upper end of housing
Equipped with feed inlet, feed inlet is located at the top of the input end of cutting channel, and the front end of housing is equipped with the port of export with cutting channel
The discharge port of connection.
The automation equipment for being used for tissue block cutting provided using the application, in use, driving two by actuating unit
A cutter shaft relatively rotates, it is only necessary to a umbilical cord China Tong Shi glue to be cut is put into feed inlet, umbilical cord China Tong Shi glue is via two
The cutting channel that set of blades is formed is cut, and is finally skidded off from discharge port.In this way, obtain required group by the way of automation
Block is knitted, it is time saving and energy saving, substantially increase the production efficiency of cutting tissue block；And the tissue block size obtained is homogeneous, also effectively carries
The high quality of production.In addition, the automation equipment that the application provides, simple in structure, design is reasonable, easy to operate, is ensureing to give birth to
Also production efficiency is effectively increased while yield and quality, it is highly practical, beneficial to being standardized production and popularization.
Preferably, the knife group in the application is detachably connected with housing, and cleaning easy to knife group, sterilize and reuse.
Wherein, the set of blades on cutter shaft includes multiple roller knife blades, it is preferred that the quantity of roller knife blade for 20~
Between 40, the quantity of rational roller knife blade can be specifically selected according to actual implementation condition；Roller knife blade is preferred to be selected not
Rust steel material be made, stainless steel has excellent corrosion resistance, mouldability, compatibility and obdurability, can easy to for a long time, have
Effect and reliable cutting operation；The shape of roller knife blade preferably selects circle, simple in structure, and processing technology is easy, beneficial to reduction
Production cost, good economy performance.
Wherein, the roller knife blade of rolling is embedded in corresponding gap blade in every group of knife group, and between embedded corresponding blade
Depth bounds in gap is 1mm~5mm, the distance of specific depth can according to the size of the magnificent Tong Shi glue tissue block of actual needs come
Carry out rational selection and setting.
Wherein, every group of knife group includes multiple roller knife blades, the spacing range between two neighboring roller knife blade for 1mm~
2mm, the distance of specific depth can be carried out according to the magnificent Tong Shi glue tissue block of actual needs or the size of other biological tissue's blocks
Rational selection and setting.
Further, knife group further includes two groups of knife combs, and two groups of knife combs are corresponded with two set of blades being arranged on cutter shaft
Set；There is empty slot between the roller knife blade of set of blades, in the empty slot between the knife comb insertion roller knife blade being correspondingly arranged；Knife comb
Installation end is fixed on the upper end of housing, and the broach of knife comb extends downward into the bottom of set of blades.Wherein, the installation end of knife comb can lead to
Cross rivet or bolt or the mode of bonding is fixedly connected with the upper end of housing, can specifically be selected rationally according to actual implementation condition
Connection mode.
In the present embodiment, knife comb includes the crossbeam as installation end and the broach being fixed on crossbeam, wherein, broach
Cross sectional shape is in broken line type, and broach is bent downward.One end that knife is combed is fixed on to the upper end of housing, the other end extends to blade
The bottom of group, can concentrate the magnificent Tong Shi glue tissue block after chopping and be dropped by two knives comb bottom centre position, be dropped easy to cut
Magnificent Tong Shi glue tissue block collection.
Further, be additionally provided with the tablet being obliquely installed in housing, tablet is located at the lower section of knife comb, and one end of tablet with
Cutting channel connects, and the other end is connected with discharge port, to form the transfer passage for being used for conveying tissue block.In the present embodiment,
Tablet is made of stainless steel, and stainless steel has excellent corrosion resistance, mouldability, compatibility and obdurability, with
Effectively prevent from having an impact the magnificent Tong Shi glue tissue block after cutting, and then to ensure mescenchymal stem cell during follow-up cultivation
The quality and quantity separated out from magnificent Tong Shi glue middle reaches.
Particularly, the angle of inclination of tablet is preferably 60 degree, to ensure the smooth sliding of the magnificent Tong Shi glue tissue block after cutting
Go out.Certainly, the angle of inclination of tablet may be alternatively provided as other angles, specifically can root such as the unspecified angle between 30 degree~80 degree
Factually border implementation condition carries out rational selection and setting.
Specifically, wherein one end of a cutter shaft is connected with actuating unit, the other end is equipped with main linkage grinding tooth wheel 5；It is another
The end of root cutter shaft is equipped with the duplicate invoice being meshed with main linkage grinding tooth wheel 5 and moves grinding tooth wheel 6.One knife group includes two relative rotations
Cutter shaft, wherein a cutter shaft, as active rotation axis, the roller knife blade on active rotation axis is denoted as drive roll knife；It is another
For root cutter shaft as driven rotation axis, the roller knife blade in driven rotation axis is denoted as driven voller knife；Active rotation axis and power
Mechanism connects, and actuating unit driving active rotation axis rotates, and active rotation axis moves grinding tooth wheel by main linkage grinding tooth wheel 5 with duplicate invoice
6 engagements, drive driven rotation axis to rotate, simple in structure, and transmission effect is good.
Specifically, actuating unit includes motor 1 and transmission component, and transmission component includes main chain travelling gear 2 and passes through
The secondary chain transmission gear 4 that driving chain 3 is connected with main chain travelling gear 2, main chain travelling gear 2 and the drive shaft of motor 1 connect
Connect, secondary chain transmission gear 4 is connected with the cutter shaft equipped with main linkage grinding tooth wheel 5.Motor 1 drives main chain travelling gear 2 to rotate, into
And driving driving chain 3 to rotate, driving chain 3 drives secondary chain transmission gear 4 to rotate, and secondary chain transmission gear 4 drives above-mentioned active
Rotation axis rotates, and active rotation axis, which rotates, drives driven rotation axis to rotate corresponding thereto, and then forms for cutting umbilical cord China
The cutting channel of Tong Shi glue, the degree of automation is high, greatlys save human resources, improves production efficiency, while is also beneficial to protect
Demonstrate,prove the quality of production.
In the present embodiment, secondary chain transmission gear 4 is connected by rotating pin 8 with the cutter shaft equipped with main linkage grinding tooth wheel 5,
Using the connection mode for rotating pin, simple in structure, simple installation.Opposite, the cutter shaft of grinding tooth wheel 6 is moved remote equipped with duplicate invoice
The end that duplicate invoice moves grinding tooth wheel 6 is equipped with rotation pin 13, and is equipped with corresponding pin support plate and pressing plate in the end, with the same time
Support force is provided to rotate pin 8 and rotating pin 13, and then ensures the connection reliability of cutter shaft and actuating unit, is also beneficial to prolong
The service life of long whole automation equipment.
Preferably, grinding tooth wheel company is moved in the end of two cutter shafts with main linkage grinding tooth wheel and duplicate invoice respectively by rotating pin
Connect.Specifically, in the present embodiment, active rotation axis is connected by rotating pin 11 with main linkage grinding tooth wheel, driven rotation axis
Grinding tooth wheel is moved by rotating pin 16 and duplicate invoice to be connected, using the connection mode for rotating pin, simple in structure, simple installation.
Further, the first support plate 20 and the first pressing plate 21 in the first support plate 20 are additionally provided with housing, the
The end of one support plate 20 and the first pressing plate 21 offers groove portion, and the first support plate 20 and the groove portion of the first pressing plate 21 are common
The slide opening for passing through and rotating for rotating pin is limited, the first pressing plate 21 is fixed by fastener and the first support plate 20 to be connected
Connect.To ensure that two cutter shafts move the connection reliability of grinding tooth wheel with main linkage grinding tooth wheel and duplicate invoice respectively, it is equipped with and is used in housing
Support rotating pin 11 and the first support plate 20 for rotating pin 16, specifically, the top edge of the first support plate 20 corresponds to respectively
Semicircular groove portion is offered in rotating pin 11 and rotating pin 16, the lower edge of the first pressing plate 21 corresponds respectively to rotational pin
Son 11 and rotation pin 16 offer semicircular groove portion, and the first support plate 20 is inserted when will rotate pin 11 and rotate pin 16
Groove portion in after, then the first pressing plate 21 is assembled in the first support plate 20, and the first pressing plate 21 is by fastener, to realize it
With being fixedly connected for the first support plate 20.
Preferably, fastener can be screw or bolt and fixing nut.In the present embodiment, bolt and fixed spiral shell are selected
The mode of mother 22 fixes the first support plate 20 and the first pressing plate 21, wherein, the first support plate 20 and the first pressing plate 21 are equipped with peace
Hole is filled, is fastened after two mounting holes of screw bolt passes by fixing nut 22, simple in structure, easy to operate and fixed effect
It is good.
Preferably, the quantity of knife group is two or more, and multiple knife groups are set gradually along the axis direction of cutter shaft,
And the cutter shaft of two adjacent knife groups is connected by connecting pins.It should be noted that the quantity of knife group can be one, or two
Or two or more, specifically can reasonably it be set according to actual implementation situation.When the quantity of knife group is two or more
When, multiple knife groups are set gradually with the axis direction of the central shaft where cutter shaft, logical between its two knife group being disposed adjacent
Cross connecting pins connection.
In the present embodiment, the setting quantity of knife group is preferably two groups, i.e. the first knife group and the second knife group, the first knife group and
Second knife group includes the cutter shaft of two relative rotations；Wherein, the active rotation axis of the first knife group passes through connecting pins 9 and second
The active rotation axis connection of knife group, the driven rotation axis that the driven rotation axis of the first knife group passes through 14 and second knife group of connecting pins
Connection, simple in structure, simple installation.
Further, the second support plate 17 and the second pressing plate 18 in the second support plate 17 are additionally provided with housing, the
The end of two support plates 17 and the second pressing plate 18 offers groove portion, and the second support plate 17 and the groove portion of the second pressing plate 18 are common
The through hole for passing through and rotating for connecting pins is limited, the second pressing plate 18 is fixed by fastener and the second support plate 17 to be connected
Connect.To ensure the connection reliability of two cutter shafts of two cutter shafts of the first knife group A and the second knife group B, it is equipped with and is used in housing
The second support plate 17 of connecting pins 9 and connecting pins 14 is supported, specifically, the top edge of the second support plate 17 corresponds respectively to
Connecting pins 9 offer semicircular groove portion with connecting pins 14, and the lower edge of the second pressing plate 18 corresponds respectively to connecting pins 9
Semicircular groove portion is offered with connecting pins 14, when the groove that connecting pins 9 and connecting pins 14 are inserted to the second support plate 17
After in portion, then the second pressing plate 18 is assembled in the second support plate 17, and the second pressing plate 18 is by fastener, to realize itself and
Two support plates 17 are fixedly connected.
Preferably, fastener can be screw or bolt and fixing nut.In the present embodiment, bolt and fixed spiral shell are selected
The mode of mother 19 fixes the second support plate 17 and the second pressing plate 18, wherein, the second support plate 17 and the second pressing plate 18 are equipped with peace
Hole is filled, is fastened after two mounting holes of screw bolt passes by fixing nut 19, simple in structure, easy to operate and fixed effect
It is good.
Specifically, the quantity of feed inlet, discharge port is consistent with the quantity of knife group, and feed inlet, discharge port and cutting channel
Correspond and set, prevent the tissue block of not homologous umbilical cord China Tong Shi glue from obscuring.In the present embodiment, the quantity of knife group
For two, for this reason, corresponding, the quantity of feed inlet is also two, i.e. feed inlet A1 and feed inlet B1；Transfer passage, discharging
The quantity of mouth is also two, i.e., including tablet 27 and tablet 28；Wherein, tablet 27 is set corresponding to feed inlet A1, and tablet 28 is right
It should be set in feed inlet B1.In addition, the quantity of knife comb is four, i.e., 25 and knife comb 26 are combed including knife comb 23, knife comb 24, knife, its
In, the drive roll knife 7 that knife comb 23 corresponds on the main rotating shaft of the first knife group is set；Knife comb 24 correspond to the first knife group from rotation
Driven voller knife 12 in shaft is set；The drive roll knife 10 that knife comb 25 corresponds on the main rotating shaft of the second knife group is set；Knife is combed
The 26 driven voller knives 15 corresponded in the slave rotation axis of the second knife group are set.
Provided by the present invention for the automation equipment of tissue block cutting, the time of acquisition China Tong Shi glue tissue blocks can be made big
It is big to shorten, a umbilical cord China Tong Shi glue cutting can be finished in 1 minute, the tissue block of acquisition is uniform in size, consistent.And this
The automation equipment that application provides is furnished with two knife groups, can cut the umbilical cord China Tong Shi glue in two different puerpera sources at the same time, cut
The process of cutting is not interfere with each other, and the tissue block of acquisition will not be obscured.Meanwhile each knife group be it is detachable, if desired
More umbilical cord China Tong Shi glue tissues are handled, after cutter assembling and dismantling are unloaded down, after conventional cleaning, disinfection, assembling, will can be remained immediately
Cut in remaining umbilical cord China Tong Shi glue feeding devices, on a large scale from umbilical cord China Tong Shi glue extract mescenchymal stem cell and
Speech, the quality for obtaining China's Tong Shi glue tissue blocks is greatly improved using the device, improves work efficiency, is increased and is filled between repeatedly preparing
The uniformity of matter stem cell.Collect the magnificent Tong Shi glue tissue block obtained and prepare umbilical cord mesenchymal stem cells according to adherent method, favorably
Dissociate in tissue umbilical cord mesenchymal stem cells in the block, and do not cause tissue block adherent loosely to float, it is final discarded.
The cutting step of the automation equipment for being used for tissue block cutting provided using the application, wherein, the quantity of knife group
For two, specifically include：
1st, motor 1 is started, motor 1 drives main chain travelling gear 2 to rotate, and main chain travelling gear 2 passes through driving chain 3
4 backwards rotation of secondary chain transmission gear is driven, main linkage grinding tooth wheel 5 rotates in same direction with secondary chain transmission gear 4, is engaged by gear,
Drive duplicate invoice to move grinding tooth wheel 4 to rotate backward.Drive roll knife 7 and 10 in two groups of knife groups A and B inversely turns with main linkage grinding tooth wheel 5
Dynamic, driven voller knife 12 and 15 move grinding tooth wheel 6 with duplicate invoice and oppose rolling movement.
2nd, will be about the magnificent Tong Shi glue of 2~3cm from the feed inlet A1 of first group of knife group A or/and second group of knife group B or/and
B1 is put into, and the blade through drive roll knife 7 or/and 10, driven voller knife 12 or/and 15 is vertically cut, and magnificent Tong Shi glue is cut into bar,
Tablet 27 or/and tablet 28 of the gained China Tong Shi adhesive tape through knife comb lower section are skidded off and are collected in corresponding discharge outlet.
3rd, the magnificent Tong Shi adhesive tape of finishing collecting, it is in 90 degree of vertical angles to make magnificent Tong Shi adhesive tape and roller knife blade, by magnificent Tong Shi
Adhesive tape is put into again, and the blade again through drive roll knife 7 or/and 10, driven voller knife 12 or/and 15 is vertically cut, and can be incited somebody to action at this time
It is 1~2mm that magnificent Tong Shi adhesive tape, which is cut into size,3Tissue block, tissue block skids off through tablet 27 or/and tablet 28 and accordingly going out
It is collected at material mouth.
In addition, before cutting obtains China's Tong Shi glue tissue blocks, step is further included：
1st, umbilical cord China Tong Shi glue separates
A, under gnotobasis, umbilical cord tissue is placed in sample memotron, Sample preservation liquid is added in memotron so that is protected
Liquid storage submerges umbilical cord tissue, and sample memotron is placed in 4~8 DEG C of transport cases, interior when 24 is small to send to GMP standard laboratories
Reason；
B, after sample memotron being sprayed 75% alcohol disinfecting, it is put into Biohazard Safety Equipment, umbilical cord group is taken out with tissue clamps
Knit, be placed in Tissue Culture Dish, be immersed in about 30s in 75% alcohol, then umbilical cord tissue is cleaned 2~4 times with PBS buffer；
C, umbilical cord tissue is cut to the segment that length is 2~3cm, the artery and venous blood in tissue is pressed with tissue clamps
Pipe, makes blood discharge as far as possible, is cleaned 2~4 times with PBS buffer；
D, eye scissors is used cooperatively tissue clamps, removes arteries and vein blood vessel in umbilical cord tissue segment, up to China
Tong Shi glue.
The automation equipment for being used for tissue block cutting provided using the application, umbilical cord mesenchymal stem cells preparation process,
Mainly include：
A, China's Tong Shi glue cleans 1~2 time through PBS buffer；
B, device for opening power supply, magnificent Tong Shi glue segment is put into automation equipment, collects and arranges magnificent Tong Shi adhesive tape, make
Be in 90 degree of vertical angles with blade, put into again in automation equipment, you can obtain China Tong Shi glue tissue blocks；
C, China's Tong Shi glue tissue blocks are collected into culture dish with spoon, it is with tissue clamps that tissue block is evenly laid out in culture dish
Bottom, by culture dish in 37 DEG C, 5%CO2When culture 2~4 is small in incubator, cell culture medium is then added, makes culture medium firm
Tissue block just was submerged, in 37 DEG C, 5%CO2Continue to cultivate in incubator；
When D, cultivating the 6th day and the 10th day, half amount replaces cell culture medium respectively, in cell fusion degree up to 80%~90%
When, harvest cell carries out secondary culture.
In conclusion the utility model provides a kind of automation equipment for tissue block cutting, including housing and set
In the cutting mechanism in housing；Cutting mechanism includes knife group and drives the actuating unit that movable cutter set rotates, and knife group includes two relatively
The cutter shaft of rotation, is equipped with the set of blades being vertically arranged on every cutter shaft, and the roller knife blade of the set of blades on two cutter shafts is in
Interlaced setting, forms the cutting channel for being used for cutting tissue block；The upper end of housing is equipped with feed inlet, and feed inlet is positioned at cutting
The top of the input end of passage, the front end of housing are equipped with the discharge port connected with the port of export of cutting channel.What the application provided
Simple in structure for cutting the device of tissue block, design is reasonable；In use, drive two cutter shafts are opposite to turn by actuating unit
It is dynamic, it is only necessary to tissue to be cut to be put into feed inlet, the cutting channel that tissue to be cut is formed via two set of blades carries out
Cutting, finally skids off from discharge port.In this way, obtaining required tissue block by the way of automation, cutting speed is fast, time saving province
Power, production efficiency are high；And the tissue block that cutting obtains is uniform in size, consistent, the quality of production is effectively increased, easy to subsequently organize
Block is adherent to be operated with culture, highly practical, beneficial to being standardized production and popularization.
Finally it should be noted that：Above example is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：
It can still modify the technical solution described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the utility model technology
The spirit and scope of scheme.
Claims (10)
- A kind of 1. automation equipment for tissue block cutting, it is characterised in that：Cutting including housing and in the housing Cutting mill structure；The cutting mechanism includes the actuating unit that knife group and the driving knife group rotate, and the knife group includes two relatively The cutter shaft of rotation, is equipped with the set of blades being vertically arranged, and the roller of the set of blades on two cutter shafts on the every cutter shaft Knife blade is in interlaced setting, forms the cutting channel for being used for cutting tissue block；The upper end of the housing is equipped with feed inlet, institute The top that feed inlet is located at the input end of the cutting channel is stated, the front end of the housing is equipped with the outlet with the cutting channel Hold the discharge port of connection.
- 2. the automation equipment according to claim 1 for tissue block cutting, it is characterised in that：The knife group further includes Two groups of knife combs, knife comb is corresponded with two set of blades being arranged on the cutter shaft and set described in two groups；The roller of the set of blades There is empty slot between knife blade, the knife comb being correspondingly arranged is inserted into the empty slot between the roller knife blade；The peace of the knife comb Dress end is fixed on the upper end of the housing, and the broach of the knife comb extends downward into the bottom of the set of blades.
- 3. the automation equipment according to claim 2 for tissue block cutting, it is characterised in that：Also set in the housing There is the tablet being obliquely installed, the tablet is located at the lower section of knife comb, and one end of the tablet connects with the cutting channel Logical, the other end is connected with the discharge port, to form the transfer passage for being used for conveying the tissue block.
- 4. the automation equipment according to claim 1 for tissue block cutting, it is characterised in that：A wherein knife One end of axis is connected with the actuating unit, and the other end is equipped with main linkage grinding tooth wheel；The end of another cutter shaft be equipped with The duplicate invoice that the main linkage grinding tooth wheel is meshed moves grinding tooth wheel.
- 5. the automation equipment according to claim 4 for tissue block cutting, it is characterised in that：The actuating unit bag Motor and transmission component are included, the transmission component includes main chain travelling gear and passes through driving chain and the main chain driving cog Take turns the secondary chain transmission gear of connection, the driving axis connection of the main chain travelling gear and the motor, the pair chain drive tooth Take turns and be connected with the cutter shaft equipped with the main linkage grinding tooth wheel.
- 6. the automation equipment according to claim 4 for tissue block cutting, it is characterised in that：Two cutter shafts Move grinding tooth wheel with the main linkage grinding tooth wheel and the duplicate invoice respectively by rotating pin and be connected in end.
- 7. the automation equipment according to claim 6 for tissue block cutting, it is characterised in that：Also set in the housing There are the first support plate and the first pressing plate in first support plate, the end of first support plate and the first pressing plate is equal Groove portion is offered, and first support plate and the groove portion of the first pressing plate are limited for being passed through simultaneously for the rotation pin jointly The slide opening of rotation, first pressing plate are fixedly connected by fastener with first support plate.
- 8. the automation equipment according to claim 1 for tissue block cutting, it is characterised in that：When the number of the knife group Measure for two or more when, multiple knife groups are set gradually along the axis direction of the cutter shaft, and adjacent two knives The cutter shaft of group is connected by connecting pins.
- 9. the automation equipment according to claim 8 for tissue block cutting, it is characterised in that：Also set in the housing There are the second support plate and the second pressing plate in second support plate, the end of second support plate and the second pressing plate is equal Groove portion is offered, and second support plate and the groove portion of the second pressing plate are limited for being passed through simultaneously for the connecting pins jointly The through hole of rotation, second pressing plate are fixedly connected by fastener with second support plate.
- 10. the automation equipment for being used for tissue block cutting according to claim 8 or claim 9, it is characterised in that：The charging Mouth, the quantity of discharge port are consistent with the quantity of the knife group, and the feed inlet, discharge port are corresponded with the cutting channel Set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721184805.1U CN207240299U (en) | 2017-09-15 | 2017-09-15 | A kind of automation equipment for tissue block cutting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721184805.1U CN207240299U (en) | 2017-09-15 | 2017-09-15 | A kind of automation equipment for tissue block cutting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207240299U true CN207240299U (en) | 2018-04-17 |
Family
ID=61889003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721184805.1U Expired - Fee Related CN207240299U (en) | 2017-09-15 | 2017-09-15 | A kind of automation equipment for tissue block cutting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207240299U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN110281302A (en) * | 2019-06-28 | 2019-09-27 | 西安医学院 | A kind of Soft tissue cutting instrument |
| CN113967931A (en) * | 2021-10-20 | 2022-01-25 | 张斌 | Umbilical cord tissue cutting machine |
| CN115804856A (en) * | 2023-02-08 | 2023-03-17 | 天津长和生物技术有限公司 | Instrument degassing unit after umbilical cord tissue cutting |
-
2017
- 2017-09-15 CN CN201721184805.1U patent/CN207240299U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486043A (en) * | 2018-02-02 | 2018-09-04 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN108486043B (en) * | 2018-02-02 | 2019-03-19 | 中南大学湘雅医院 | A kind of method of cartilaginous tissue chopping, enzymolysis, digestion integrated device and cartilaginous tissue processing |
| CN110281302A (en) * | 2019-06-28 | 2019-09-27 | 西安医学院 | A kind of Soft tissue cutting instrument |
| CN113967931A (en) * | 2021-10-20 | 2022-01-25 | 张斌 | Umbilical cord tissue cutting machine |
| CN115804856A (en) * | 2023-02-08 | 2023-03-17 | 天津长和生物技术有限公司 | Instrument degassing unit after umbilical cord tissue cutting |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207240299U (en) | A kind of automation equipment for tissue block cutting | |
| CN211445690U (en) | A collection system for placenta hematopoietic stem cell | |
| CN107211663B (en) | A kind of livestock-raising High-efficiency straw crushing device | |
| CN206277420U (en) | A kind of umbilical cord scissors | |
| CN107012848A (en) | A kind of water conservancy algae-removing device | |
| CN210477096U (en) | Filter cake mincer after centrifugal treatment in drug production | |
| CN205216993U (en) | Tissue cutting and crushing device for separating living cells | |
| CN202172618U (en) | Mulberry leaf cutting knife | |
| CN105998080A (en) | Earthworm processing device and earthworm processing method | |
| CN103586912B (en) | Automatic kudzu vine root slicing machine | |
| CN112544209B (en) | Harvesting processing equipment capable of automatically collecting and processing corn straws | |
| CN107690995A (en) | A kind of pig feed feed cutter device | |
| CN209919971U (en) | Tissue mass shears for culturing primary cells | |
| CN203828013U (en) | Peeling device for tuber crops | |
| CN218342353U (en) | Wicker peeling machine for wickerwork processing | |
| CN206776733U (en) | A kind of Portable tobacco leaf chopping machine | |
| CN201634683U (en) | Device for separating primary cells from tissue | |
| CN207505432U (en) | A kind of Jujun grasses cutting machine | |
| CN112661550A (en) | Device for preparing fertilizer by fermenting crop straws | |
| CN212331209U (en) | Slicing machine for planting and processing traditional Chinese medicinal materials | |
| CN206227636U (en) | A kind of agricultural sugarcane peeling cutting machine | |
| CN106863368A (en) | A kind of medical tissue crushes scissors | |
| CN210604554U (en) | Sampling inspection device is used in microbial fertilizer production | |
| CN213977715U (en) | Placenta extraction device integrating digestion and collection | |
| CN208940387U (en) | A kind of weeder being convenient to clean weeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180417 Termination date: 20210915 |