CN207114473U - A kind of capillary electrophoresis - Google Patents
A kind of capillary electrophoresis Download PDFInfo
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- CN207114473U CN207114473U CN201720998966.8U CN201720998966U CN207114473U CN 207114473 U CN207114473 U CN 207114473U CN 201720998966 U CN201720998966 U CN 201720998966U CN 207114473 U CN207114473 U CN 207114473U
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- transfer tube
- capillary
- buffer solution
- pressure pump
- bottle
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Abstract
The utility model belongs to separation detecting device technical field,It is related to a kind of capillary electrophoresis,Including insulating box,The first buffer solution bottle is provided with insulating box,Sample bottle,Second buffer solution bottle,The first transfer tube is provided with first buffer solution bottle,The second transfer tube is provided with sample bottle,The 3rd transfer tube is provided with second buffer solution bottle,First transfer tube is provided with the first high-pressure pump,Second transfer tube is provided with the second high-pressure pump,The output end of first high-pressure pump and the second high-pressure pump is connected with the input of triple valve,The output end of triple valve is connected with quantitative sample injection valve,Quantitative sample injection valve is connected with separating one end of capillary,The other end of separation capillary is connected with the 3rd transfer tube,The both ends of separation capillary are respectively arranged with electrode,High voltage power supply is provided between electrode,Window is offered on separation capillary,The light path of detector is relative with window,Detector is connected with data recording and processing instrument.The utility model is simple in construction, easy to use；Improve the reappearance of separation detection.
Description
Technical field
The utility model belongs to separation detecting device technical field, is related to a kind of capillary electrophoresis.
Background technology
So-called electrophoresis, refers to the motion of charged particle in the electric field, different material by electrically charged and molecular weight not
Together, therefore in the electric field movement velocity is different, according to this with feature, using electrophoresis can with different material is carried out it is qualitative or
Quantitative analysis, or certain mixture is subjected to component analysis or the extraction preparation of single component, this is in clinical examination or experimental study
In be extremely important.Electrophoresis apparatus is based on what above-mentioned principle manufactured and designed.Electrophoresis is generally divided into free interface electricity
Swimming and the major class of zone electrophoresis two, free boundary electrophoresis are not required to holder, such as isoelectric focusing electrophoresis, isotachophoresis, density gradient electricity
Swimming and microelectrophoresis etc., this kind of electrophoresis has been rarely employed at present；Zone electrophoresis all uses supporting dielectric, different according to supporting dielectric
It is divided into paper electrophoresis, cellulose acetate membrane electrophoresis, thin layer electrophoresis and gel electrophoresis etc., in addition, it is different according to the device form of supporting dielectric,
Zone electrophoresis can be divided into again horizontal board-like electrophoresis, vertical board-like electrophoresis, vertical disk electrophoresis, Capillary Electrophoresis, bridge shape electrophoresis and
Continuous flow electrophoresis etc..
Capillary Electrophoresis is one of analysis method with fastest developing speed in recent years, and Capillary Electrophoresis is also known as efficient capillary electricity
Swimming, it is a kind of novel liquid-phase isolation technics using capillary as split tunnel, using high-voltage dc as driving force.Capillary electricity
Swimming skills art is widely used in the identification of seed protein.Traditional SDS-PAGE, 2-DE method is due in glue, electrophoresis and colour developing ring
Section takes considerable time also there is strict demand to test sample amount, it is difficult to realizes that high flux scans.Capillary Electrophoresis
(CapillaryElectrophoresis, CE) then can effectively make up above mentioned problem, micro, quick and increasingly automated etc. with its
Feature, great superiority is shown in terms of the batch identification of protein.
Simple, convenient and rapid Quality Wheat high-molecular-weight glutelin subunit (HMW-GS) can be identified using capillary electrophoresis technique, soon
It is prompt sensitive, it efficiently can quickly determine the high-molecular-weight glutelin subunit combination of a large amount of different cultivars.The device used at present is general
Store-through is complicated, the problem of separation detection poor reproducibility, therefore researches and develops Capillary Electrophoresis simple in construction, reproducibility is high
Device seems most important.
Utility model content
The purpose of this utility model is to provide a kind of capillary electrophoresis, solves Capillary Electrophoresis dress in the prior art
The problem of putting complicated, separation detection poor reproducibility.
The utility model is the technical scheme adopted is that a kind of capillary electrophoresis, including insulating box, is set in insulating box
It is equipped with the first buffer solution bottle, sample bottle, the second buffer solution bottle, the first transfer tube, the second transfer tube, the 3rd transfer tube, first high
Press pump, the second high-pressure pump, triple valve, separation capillary, quantitative sample injection valve, electrode and detector；
The first transfer tube is provided with first buffer solution bottle, the second transfer tube is provided with sample bottle, is set in the second buffer solution bottle
There is the 3rd transfer tube, the first transfer tube is provided with the first high-pressure pump, and the second transfer tube is provided with the second high-pressure pump, the first high-pressure pump
It is connected with the output end of the second high-pressure pump with the input of triple valve, the output end of triple valve is connected with quantitative sample injection valve,
Quantitative sample injection valve is connected with separating one end of capillary, and the other end for separating capillary is connected with the 3rd transfer tube, separation
The both ends of capillary are respectively arranged with electrode, and high voltage power supply is provided between two electrodes, and high voltage power supply is connected with two electrodes, point
From window is offered on capillary, the light path of detector is relative with window, and detector is connected with data recording and processing instrument.
The characteristics of the utility model, also resides in,
Buffer solution, buffer solution H are respectively arranged with wherein the first buffer solution bottle and the second buffer solution bottle3PO4- Glycine is buffered
Liquid.Wherein H3PO4- Glycine buffer solutions are 20%ACN+0.4%Glycine+0.05%HPMC.Wherein buffer solution phosphoric acid is molten
It is 2.5 that liquid (85%), which adjusts pH,.
Wherein separation capillary is that detection length is 37cm, and internal diameter is 50 μm of quartzy non-coatings capillary pipe.
Wherein detector is photodiode array detector.
Temperature wherein in insulating box is 30 DEG C.
The beneficial effects of the utility model are：It is simple in construction, it is easy to use；The input mode of quantitative sample injection valve makes sample size
Accurately, repeatability is high, coordinates the system of buffer solution to significantly improve the reappearance of separation detection；By the way that buffer solution and sample are divided
For two-way input make retest same sample test twice between operation become simple and convenient, improve detection efficiency, together
When improve the reappearance of separation detection.
Brief description of the drawings
Fig. 1 is a kind of structural representation of capillary electrophoresis of the utility model.
Description of reference numerals：
1. insulating box, 2. first buffer solution bottles, 3. sample bottles, 4. second buffer solution bottles, 5. first transfer tubes, 6. second pass
Defeated pipe, 7. the 3rd transfer tubes, 8. first high-pressure pumps, 9. second high-pressure pumps, 10. triple valves, 11. separation capillaries, 12. quantitatively enter
Sample valve, 13. electrodes, 14. detectors, 15. high voltage power supplies, 16. data recording and processing instrument.
Embodiment
Below in conjunction with the accompanying drawings, an embodiment of the present utility model is described in detail, it is to be understood that this
The protection domain of utility model is not limited by embodiment.
A kind of capillary electrophoresis, as shown in figure 1, including insulating box 1, the first buffer solution bottle is provided with insulating box 1
2nd, sample bottle 3, the second buffer solution bottle 4, the first transfer tube 5, the second transfer tube 6, the 3rd transfer tube 7, the first high-pressure pump 8, second
High-pressure pump 9, triple valve 10, separation capillary 11, quantitative sample injection valve 12, electrode 13 and detector 14；
The first transfer tube 5 is provided with first buffer solution bottle 2, the second transfer tube 6, the second buffer solution bottle 4 are provided with sample bottle 3
In be provided with the 3rd transfer tube 7, the first transfer tube 5 is provided with the first high-pressure pump 8, and the second transfer tube 6 is provided with the second high-pressure pump 9,
First high-pressure pump 8 is connected with the output end of the second high-pressure pump 9 with the input of triple valve 10, and the output end of triple valve 10 is with determining
Amount sampling valve 12 is connected, and quantitative sample injection valve 12 is connected with separating one end of capillary 11, separates the other end of capillary 11
It is connected with the 3rd transfer tube 7, the both ends of separation capillary 11 are respectively arranged with electrode 13, and height is provided between two electrodes 13
Voltage source 15, high voltage power supply 15 are connected with two electrodes 13, are separated and are offered window, the light path and window of detector 14 on capillary 11
Mouth is relative, and detector 14 is connected with data recording and processing instrument 16.
The input mode of quantitative sample injection valve makes sample size accurate, repeated high.By the way that buffer solution and sample are divided into two-way
Input make retest same sample test twice between operation become simple and convenient, improve detection efficiency, improve simultaneously
The reappearance of separation detection.
Buffer solution, buffer solution H are respectively arranged with wherein the first buffer solution bottle 2 and the second buffer solution bottle 43PO4- Glycine delays
Fliud flushing.Wherein H3PO4- Glycine buffer solutions are 20%ACN+0.4%Glycine+0.05%HPMC.Wherein buffer solution phosphoric acid
It is 2.5 that solution (85%), which adjusts pH,.The input mode of quantitative sample injection valve coordinates the system of buffer solution to significantly improve separation detection
Reappearance.
Wherein separation capillary 11 is that detection length is 37cm, and internal diameter is 50 μm of quartzy non-coatings capillary pipe.
Wherein detector 14 is photodiode array detector.
Temperature wherein in insulating box 1 is 30 DEG C.
Wherein the separation voltage of separation detection is 12.5kv.
Capillary Electrophoresis be using capillary as split tunnel, using high-voltage dc as driving force, according to each group in sample
/ mobility and difference in distribution behavior and realize the electrophoretic separation analysis method of separation.Principle：Solution is analysed to draw
Enter capillary sample inlet one end, after applying DC voltage, each component flows to capillary by the vector of respective electrophoresis stream and EOF
The pipe port of export, by the order of cation, neutral ion and anion and its electric charge size by detector, neutral component is each other not
Can separation.
In use, open insulating box 1 is arranged to 30 DEG C by its internal temperature, in the first buffer solution bottle 2 and the second buffer solution
Buffer solution is added in bottle 4, testing sample is added in sample bottle 3, opens the first high-pressure pump 8, buffer solution is buffered from first
The first transfer tube 5 is sucked in liquid bottle 2, triple valve 10 is opened to relevant position, buffer solution and entered in whole separation capillary 11,
Then the second high-pressure pump 9 is opened, sample is sucked to the second transfer tube 6 from sample bottle 3, triple valve 10 is opened into corresponding positions
Put, sample size is controlled with quantitative sample injection valve 12, sample enters the entrance point of separation capillary 11, opens high voltage power supply 15, separation
Voltage is 12.5kv, and each component is pressed by the port of export of the vector of respective electrophoresis stream and EOF flow direction separation capillary 11
Ion characteristic successively carries out detection separation by detector 14.
In summary, a kind of capillary electrophoresis that the utility model embodiment provides is simple in construction, easy to use；It is fixed
The input mode of amount sampling valve 12 makes sample size accurate, repeated high, coordinates the system of buffer solution to significantly improve separation detection
Reappearance；By by buffer solution and sample be divided into two-way input make retest same sample test twice between operation become
Must be simple and convenient, detection efficiency is improved, while improve the reappearance of separation detection.
Disclosed above is only specific embodiment of the utility model, and still, the utility model embodiment is not limited to
This, the changes that any person skilled in the art can think of should all fall into the scope of protection of the utility model.
Claims (4)
1. a kind of capillary electrophoresis, it is characterised in that including insulating box (1), it is slow that first is provided with the insulating box (1)
Fliud flushing bottle (2), sample bottle (3), the second buffer solution bottle (4), the first transfer tube (5), the second transfer tube (6), the 3rd transfer tube
(7), the first high-pressure pump (8), the second high-pressure pump (9), triple valve (10), separation capillary (11), quantitative sample injection valve (12), electrode
And detector (14) (13)；
The first transfer tube (5) is provided with the first buffer solution bottle (2), the second transfer tube (6) is provided with the sample bottle (3),
The 3rd transfer tube (7) is provided with the second buffer solution bottle (4), first transfer tube (5) is provided with the first high-pressure pump (8),
Second transfer tube (6) is provided with the output end of the second high-pressure pump (9), first high-pressure pump (8) and the second high-pressure pump (9)
It is connected with the input of triple valve (10), the output end of the triple valve (10) is connected with quantitative sample injection valve (12), described
Quantitative sample injection valve (12) is connected with separating one end of capillary (11), and the other end of the separation capillary (11) and the 3rd passes
Defeated pipe (7) is connected, and the both ends of the separation capillary (11) are respectively arranged with electrode (13), between two electrodes (13)
High voltage power supply (15) is provided with, the high voltage power supply (15) is connected with two electrodes (13), is opened up on the separation capillary (11)
There is window, the light path of the detector (14) is relative with window, and the detector (14) is connected with data recording and processing instrument (16).
2. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the first buffer solution bottle (2) and the
Buffer solution is respectively arranged with two buffer solution bottles (4).
3. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the separation capillary (11) is detection
Length is 37cm, and internal diameter is 50 μm of quartzy non-coatings capillary pipe.
4. a kind of capillary electrophoresis as claimed in claim 1, it is characterised in that the detector (14) is the pole of photoelectricity two
Pipe array detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720998966.8U CN207114473U (en) | 2017-08-10 | 2017-08-10 | A kind of capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720998966.8U CN207114473U (en) | 2017-08-10 | 2017-08-10 | A kind of capillary electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207114473U true CN207114473U (en) | 2018-03-16 |
Family
ID=61578460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720998966.8U Expired - Fee Related CN207114473U (en) | 2017-08-10 | 2017-08-10 | A kind of capillary electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207114473U (en) |
-
2017
- 2017-08-10 CN CN201720998966.8U patent/CN207114473U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180316 Termination date: 20180810 |
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| CF01 | Termination of patent right due to non-payment of annual fee |