CN207103310U - A kind of gene disruption device - Google Patents
A kind of gene disruption device Download PDFInfo
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- CN207103310U CN207103310U CN201720978745.4U CN201720978745U CN207103310U CN 207103310 U CN207103310 U CN 207103310U CN 201720978745 U CN201720978745 U CN 201720978745U CN 207103310 U CN207103310 U CN 207103310U
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Abstract
Field of gene detection is the utility model is related to, it includes machine frame to specially a kind of gene disruption device, wiper mechanism, hitch, liquor-transferring system and controller in framework；The wiper mechanism includes：Rinse bath, the multiple transducers for being arranged on bottom of rinse bath and multiple shakes for being arranged on bottom of rinse bath, the rinse bath is interior to be equipped with horizontally disposed double-edged fine-toothed comb；The utility model completes the function of hundreds thousand of equipment with supersonic wave cleaning machine combination jarring machine device can, and can be according to the size of selection genetic fragment come the mode of operation of determining device, equipment investment and cost of use are so substantially reduced, the task that can also be directed to carries out more applicability regulations.
Description
Technical field
Field of gene detection is the utility model is related to, specially a kind of gene disruption device.
Background technology
In genetic test, the sequencing of two generations will enter Break Row to DNA, and require higher, there is specific segment ranges
It is required that typically can all use the Ultrasound Instrument of specialty, the price of this Ultrasound Instrument is typically in hundreds of thousands or so, the equipment investment used
It is all higher with use cost.But the similar equipment of principle is ultrasonic washing instrument, it is cleaned by ultrasonic instrument price at hundreds of yuan
Left and right, but interrupting for DNA is may not apply to because its ultrasonic power is low, frequency is high, want to carry out DNA's with ultrasonic cleaning instrument
Interrupt, the DNA of segment ranges required by acquisition, and the DNA after interrupting is used in the sequencing of two generations, almost one can not possibly be real
Existing thing.
Utility model content
Technical problem to be solved in the utility model is to provide that a kind of cost is low, the gene disruption device of frequency-adjustable.
To solve the above problems, technical solution adopted in the utility model is：
A kind of gene disruption device, it includes machine frame, wiper mechanism, hitch in framework, liquid relief
System and controller；The wiper mechanism includes：Rinse bath, the multiple transducers for being arranged on bottom of rinse bath and it is arranged on clear
Multiple shakes of washing trough bottom, the rinse bath is interior to be equipped with horizontally disposed double-edged fine-toothed comb；
The hitch includes：Annular air-pocket, the air pump connected with annular air-pocket, and positioned at the more of bottom of rinse bath
The individual support bar being uniformly arranged, the annular air-pocket are sleeved on the outside of rinse bath, and the annular air-pocket outer wall is fixedly installed on frame
On body, the top of the support bar is fixedly connected with bottom of rinse bath, and the bottom of the support bar is connected by spring and base tube
Connect, the support bar is fixedly connected with spring bottom, and the spring top at the top of base tube with being fixedly connected；
The annular air-pocket is located at rinse bath external wall of upper portion, and the air pump is located at framework bottom, and the annular air-pocket passes through
Tracheae connects with air pump gas outlet, and the tracheae is additionally provided with three-way air valve；
The liquor-transferring system includes：Liquid reserve tank and the two-way pump for connecting bottom of rinse bath, the liquid reserve tank is provided with excessive
Liquid mouth；The transducer, shake, air pump, three-way air valve, two-way pump and controller Electricity Federation.
Further, the controller is provided with the transducer knob and tune of regulation transducer operating transducers duration
Section shakes the sub- knob of shake of sub- operating time.
Further, on multiple transducer position bottom of rinse bath horizontal central lines, and it is uniformly arranged.
Further, shake is three, and three shake are uniformly arranged around bottom of rinse bath central point.
Further, the double-edged fine-toothed comb is arranged in rinse bath by four screw rod supporting legs, four screw rod supporting legs point
Not Wei Yu double-edged fine-toothed comb four angles, and be threadedly coupled with double-edged fine-toothed comb, surrounding and the rinse bath inwall of the double-edged fine-toothed comb agree with.
It is using beneficial effect caused by above-mentioned technical proposal：
The utility model completes the function of hundreds thousand of equipment with supersonic wave cleaning machine combination jarring machine device can, and
Equipment investment and usage charges can be so substantially reduced according to the size for choosing genetic fragment come the mode of operation of determining device
With the task that can also be directed to carries out more applicability regulations.
Annular air-pocket combines the conversion that elastic support bar solves two kinds of mode of operations, Reagent Tube is not moved
Dynamic, a table apparatus completes whole work process, is completed with reference to controller full-automatic.
The regulation being operated to transducer and shake can increase the flexible of equipment, and the transducer linearly set can
So that the in-fighting effect of equipment can be avoided more preferable, frequency is easier to control, and shake of circle distribution can form spiral vibration wave, increases
Beneficial vibrating effect, double-edged fine-toothed comb can adjust position of the double-edged fine-toothed comb in rinse bath by screw support according to the size of Reagent Tube, with
Adapt to the Reagent Tube of more sizes.
Brief description of the drawings
, below will be right in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, describe below
In accompanying drawing be some embodiments of the present utility model, for those of ordinary skill in the art, do not paying creativeness
On the premise of work, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is cross-sectional view of the present utility model；
Fig. 2 is top surface structure schematic diagram of the present utility model；
Fig. 3 is rinse bath bottom surface structural representation of the present utility model.
Wherein：1 framework, 21 rinse baths, 22 transducers, 23 shake, 24 double-edged fine-toothed combs, 25 screw rod supporting legs, 31 annular air-pockets, 32 gas
Pump, 33 support bars, 34 springs, 35 base tubes, 36 tracheaes, 37 three-way air valves, 41 liquid reserve tanks, 42 two-way pumps, 43 liquid spill-ways, 51 are changed
Can device knob, the 52 sub- knobs of shake.
Embodiment
To make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with the accompanying drawings and specific embodiment
Clear, complete description is carried out to utility model.
A kind of gene disruption device as Figure 1-3, it includes machine frame 1, the cleaning machine in framework 1
Structure, hitch, liquor-transferring system and controller；The wiper mechanism includes：Rinse bath 21, it is arranged on the more of the bottom of rinse bath 21
Individual transducer 22 and multiple shake 23 for being arranged on the bottom of rinse bath 21, the rinse bath 21 is interior to be equipped with horizontally disposed comb
Son 24；
The hitch includes：Annular air-pocket 31, the air pump 32 connected with annular air-pocket 31, and positioned at rinse bath 21
Multiple support bars 33 being uniformly arranged of bottom, the annular air-pocket 31 are sleeved on the outside of rinse bath 21, the annular air-pocket 31
Outer wall is fixedly installed in framework 1, and the top of the support bar 33 is fixedly connected with the bottom of rinse bath 21, the support bar 33
Bottom is connected by spring 34 with base tube 35, and the support bar 33 is fixedly connected with the bottom of spring 34, the top of spring 34
It is fixedly connected with the top of base tube 35；
The annular air-pocket 31 is located at the external wall of upper portion of rinse bath 21, and the air pump 32 is located at the bottom of framework 1, the annular gas
Capsule 31 is connected by tracheae 36 with the gas outlet of air pump 32, and the tracheae 36 is additionally provided with three-way air valve 37；The liquor-transferring system bag
Include：Liquid reserve tank 41 and the two-way pump 42 of connection rinse bath 21 bottom, the liquid reserve tank 41 are provided with liquid spill-way 43；The transducing
Device 22, shake 23, air pump 32, three-way air valve 37, two-way pump 42 and controller Electricity Federation.
The controller is provided with the transducer knob 51 and regulation shake of the regulation operating transducers duration of transducer 22
The sub- knob 52 of shake of 23 operating times；On multiple bottom level center lines of 22 position rinse bath of the transducer 21, and it is uniformly arranged；
Shake 23 is three, and three shake 23 are uniformly arranged around the bottom centre's point of rinse bath 21；The double-edged fine-toothed comb 24 passes through
Four screw rod supporting legs 25 are arranged in rinse bath 21, four screw rod supporting legs 25 respectively be located at double-edged fine-toothed comb four angles, and with comb
Son threaded connection, surrounding and the inwall of rinse bath 21 of the double-edged fine-toothed comb agree with.
The acute pyogenic infection of finger tip supersonic generator in the technical program of transducer 22, shake sub 23 acute pyogenic infection of finger tip concussion instruments；Above-mentioned two instrument
It is known in this field not just to be discussed in detail, also will not be unclear to acute pyogenic infection of finger tip.
The course of work：The working time for needing to set transducer knob 51 and the sub- knob 52 of shake according to interrupting first, it will comb
Son 24 is adjusted to the correct position of test tube by screw rod supporting leg 25, then starts dynamic power switch, and two-way pump 42 starts liquid storage
Liquid in case 41 is delivered in rinse bath 21；At the same time air pump 32 starts inflates into annular air-pocket 31, reaches setting pressure
After be stopped.
Now rinse bath 21 by annular air-pocket 31 hold tightly with the jam-packed of framework 1, liquid level reaches the specified location of rinse bath 21, two-way
Pump 42 is stopped, and transducer 22 is started working, and is stopped after reaching specified time, and the three-way air valve 37 on tracheae 36, which is opened, to be put
Gas；Simultaneously bi-directionally pump 42, which starts, returns to liquid in liquid reserve tank 41, and such annular air-pocket 31 unclamps, when so shake 23 works
It can just make rinse bath 21 unfettered, the support bar 33 hung using spring 34 also will not hinder rinse bath 21 to shake.
After shake son 23 and start, shake 23 works specified time, then repeatedly said process until reach cycle-index.
The top of spring 34 is connected with base tube 35, and the bottom of spring 34 is connected with the bottom of support bar 33, forms shockproof suspension, this
Sample support bar 33 when shake 23 works will not hinder its vibrations.
Controller can also be completed to set according to the description of the technical program, can be the control knot such as PLC, delay switch
Structure.
Ultrasonic cleaning machine principle mainly passes through transducer, and the acoustic energy of power ultrasonic source is converted into mechanical oscillation,
By clean tank wall by the cleaning fluid in Ultrasonic Radiation to trough.Insoluble dirt can be destroyed using cavitation effect immense pressure
And them is broken up in solution, destroy insoluble dirt and them is scattered in cleaning fluid, when group's particle is wrapped up in by greasy dirt
When and being adhered to cleaning parts surface, oil is emulsified, and solids depart from, so as to reach the purpose of cleaning parts surface purification.
Technical parameter：Volume 10L；Supersonic frequency 40KHz；Ultrasonic power 300W.
Excessively short and small Reagent Tube can be fixed with buoy, and buoy is porous thistle board, and Reagent Tube is inserted in the hole of buoy
Interior, in rinse bath 21 after emptying rate of liquid, buoy is fallen on double-edged fine-toothed comb naturally, and buoy floats naturally after liquid re-injection.
We conducted long-term experiment trial, substituted, be put into the water of ultrasonic cleaning tank using buoy, use 0.5-5ml
Pipe, configure 30 μ l system, using 90% power, the ultrasound pause in 80 seconds in instrument is cleaned by ultrasonic, then two-way pump 42
After removing liquid, the sample in Reagent Tube mixes in the presence of shake, restarts two-way pump 42 and is full of clean tank 21, transducing
Device works 60 seconds again to be suspended, and so repeatedly, is determined the number of occurrence according to the size of initial segment, is tested through agarose gel electrophoresis
Card, the DNA sample in the range of the required length-specific of two generations sequencing can be obtained.
Therefore transducer 22 and shake 23 working time respectively of son are assured that according to censorship sample, can also determined whole
Individual device cycle-index, these can test by limited number of time and not done excessive discussion.
Smash instrument using the ultrasound of specialty not only expense is higher, and time also long general 30min.Pass through technology
The change use of parameter not only reduces cost, and also saves usage time.The big of ultrasonic washing instrument can be coordinated in addition
It is small, a variety of test tubes can be placed, or beating for multiple samples can be also realized simultaneously using the special double-edged fine-toothed comb hole for meeting its size
It is disconnected.
Finally it should be noted that：Above example is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：
It can still be modified to the technical scheme described in previous embodiment, or which part technical characteristic is equal
Replace；And these modifications or replacement, the essence of appropriate technical solution is departed from the utility model embodiment technical scheme
Spirit and scope.
Claims (5)
- A kind of 1. gene disruption device, it is characterised in that：It includes machine frame (1), the cleaning machine in framework (1) Structure, hitch, liquor-transferring system and controller；The wiper mechanism includes：Rinse bath (21), the multiple transducers (22) for being arranged on rinse bath (21) bottom and setting Multiple shakes in rinse bath (21) bottom are sub (23), and horizontally disposed double-edged fine-toothed comb (24) is equipped with the rinse bath (21)；The hitch includes：Annular air-pocket (31), the air pump (32) connected with annular air-pocket (31), and positioned at rinse bath (21) multiple support bars (33) being uniformly arranged of bottom, the annular air-pocket (31) is sleeved on the outside of rinse bath (21), described Annular air-pocket (31) outer wall is fixedly installed in framework (1), and top and rinse bath (21) bottom of the support bar (33) are fixed Connection, the bottom of the support bar (33) are connected by spring (34) with base tube (35), the support bar (33) and spring (34) bottom is fixedly connected, with being fixedly connected at the top of base tube (35) at the top of the spring (34)；The annular air-pocket (31) is located at rinse bath (21) external wall of upper portion, and the air pump (32) is located at framework (1) bottom, the ring Shape air bag (31) is connected by tracheae (36) with air pump (32) gas outlet, and the tracheae (36) is additionally provided with three-way air valve (37)；The liquor-transferring system includes：Liquid reserve tank (41) and the two-way pump (42) for connecting rinse bath (21) bottom, the liquid reserve tank (41) it is provided with liquid spill-way (43)；The transducer (22), shake sub (23), air pump (32), three-way air valve (37), two-way pump (42) and controller Electricity Federation.
- A kind of 2. gene disruption device according to claim 1, it is characterised in that：The controller is provided with regulation transducing The transducer knob (51) of device (22) operating transducers duration and the sub- knob of shake (52) of sub (23) operating time of regulation shake.
- A kind of 3. gene disruption device according to claim 1, it is characterised in that：The multiple transducer (22) positions are clear On washing trough (21) bottom level center line, and it is uniformly arranged.
- A kind of 4. gene disruption device according to claim 1, it is characterised in that：The shake sub (23) is three, three The shake sub (23) is uniformly arranged around rinse bath (21) bottom centre point.
- A kind of 5. gene disruption device according to claim 1, it is characterised in that：The double-edged fine-toothed comb (24) passes through four screw rods Supporting leg (25) is arranged in rinse bath (21), and four screw rod supporting legs (25) are located at four angles of double-edged fine-toothed comb, and and double-edged fine-toothed comb respectively Threaded connection, surrounding and rinse bath (21) inwall of the double-edged fine-toothed comb agree with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720978745.4U CN207103310U (en) | 2017-08-07 | 2017-08-07 | A kind of gene disruption device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720978745.4U CN207103310U (en) | 2017-08-07 | 2017-08-07 | A kind of gene disruption device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207103310U true CN207103310U (en) | 2018-03-16 |
Family
ID=61583235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720978745.4U Active CN207103310U (en) | 2017-08-07 | 2017-08-07 | A kind of gene disruption device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207103310U (en) |
-
2017
- 2017-08-07 CN CN201720978745.4U patent/CN207103310U/en active Active
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| GR01 | Patent grant | ||
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Address after: 050000 504, Building B (A12), Building 202, Jingshi Collaborative Innovation Demonstration Park, No. 769, Taihang South Street, High tech Zone, Shijiazhuang, Hebei Patentee after: SHIJIAZHUANG BREEDING BIOTECHNOLOGY Co.,Ltd. Address before: 050000 room 611, Zone C, 6th floor, science and technology center, 136 Huanghe Avenue, high tech Zone, Shijiazhuang City, Hebei Province Patentee before: SHIJIAZHUANG BREEDING BIOTECHNOLOGY Co.,Ltd. |