CN207062285U - A kind of gene detecting kit liquid dispensing device - Google Patents
A kind of gene detecting kit liquid dispensing device Download PDFInfo
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- CN207062285U CN207062285U CN201720834259.5U CN201720834259U CN207062285U CN 207062285 U CN207062285 U CN 207062285U CN 201720834259 U CN201720834259 U CN 201720834259U CN 207062285 U CN207062285 U CN 207062285U
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- Prior art keywords
- detecting kit
- dispensing device
- gene detecting
- liquid
- liquid dispensing
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Abstract
The utility model provides a kind of gene detecting kit liquid dispensing device, more particularly to technical field of gene detection, including tank body, the tank body is made up of two layers of tank skin, cooling duct is formed between the tank skin, the tank interior is provided with support, and the support, which is provided with, matches somebody with somebody liquid bucket, it is described with agitating shaft is provided with liquid bucket, one end of the agitating shaft is provided with motor.The utility model can prepare gene detecting kit working solution at low ambient temperatures, keep the maximum activity of working solution, while the agitating device provided with only agitating shaft avoids stirring generation bubble from making Taq enzyme partial inactivation.
Description
Technical field
The utility model belongs to technical field of gene detection, and in particular to gene detecting kit liquid dispensing device.
Background technology
Conventional DNA test includes DNA microarray chip method, PCR- capillary electrophoresis, ARMS-PCR methods and fluorescence
Quantitative PCR method, this gene detecting kit use fluorescence quantitative PCR method.
Fluorescent quantitative PCR technique is the quantitative PCR skill with more high specific to grow up on the basis of round pcr
Art.Refer to add a specific fluorescence probe while adding pair of primers when PCR is expanded, the probe is a few nucleosides
Acid, both ends one reporter fluorescence group of mark and a quenching fluorescence group respectively.When probe is complete, reporter group is launched glimmering
Optical signal is quenched group absorptions；Just start when, probe be incorporated in any one of DNA it is single-stranded on；When PCR is expanded, the 5 ' of Taq enzyme
The end of end → 3 ' 5 prime excision enzyme activity degrades probe digestion, separates reporter fluorescence group and quenching fluorescence group, so as to which fluorescence is supervised
Examining system can receive fluorescence signal, i.e., often expand a DNA, just have a fluorescence molecule to be formed, realize fluorescence signal
Accumulation and PCR primer form Complete Synchronization.
When preparing the working solution of this kit, it is necessary to which the materials such as Taq enzyme, primer, probe and dNTP are prepared in proportion,
And the material such as Taq enzyme, primer, probe and dNTP all needs Cord blood, when preparing extensive work liquid, in the case of normal temperature
If overlong time can influence the activity of working solution, so low temperature prepares the enzymatic activity that can play maximum.Existing in general
Agitator tank is required for the stirring of the strength degree such as stirring vane, easily makes enzyme denaturation.
Patent liquid dispensing device (U of notification number CN 204051461), disclose a kind of liquid dispensing device, including liquid storing barrel, stirring
Device and the mixed liquid pipe of connection liquid storing barrel and agitating device, mixed liquid pipe are provided with feed hopper；Agitating device include inner tank body with
And the outer tank body around inner tank body, the coolant cavity of closing is formed between inner tank body and outer tank body；The inside of inner tank body is provided with
Agitating device, top connect with mixed liquid pipe, and bottom is provided with the mixed liquor apopore extended to below outer tank body；Agitating device includes
Roof through inner tank body is arranged on the agitating shaft inside inner tank body and the stirring vane being arranged on agitating shaft；Outer tank body
Top is provided with coolant inlet opening, and bottom is provided with coolant apopore；Mixed liquid pipe between liquid storing barrel and feed hopper is provided with water
Pump.The utility model can prevent rapidly-soluble powder floats on liquid or be adhered to the paddle exposed outside liquid
On piece, the problem of causing liquid preparation time to extend.But the device does not solve existing issue.
Therefore gene detecting kit working solution can be prepared at low ambient temperatures by being badly in need of one kind, keep the maximum of working solution
Activity, while reduce the gene detecting kit liquid dispensing device of enzyme inactivation.
Utility model content
The purpose of this utility model is to provide a kind of gene detecting kit liquid dispensing device, can match somebody with somebody at low ambient temperatures
Gene detecting kit working solution processed, keeps the maximum activity of working solution, while reduces enzyme inactivation.
The utility model provides following technical scheme：
A kind of gene detecting kit liquid dispensing device, including tank body, the tank body are made up of two layers of tank skin, the tank skin
Between form cooling duct, the tank interior is provided with support, and the support, which is provided with, match somebody with somebody liquid bucket, it is described with liquid bucket provided with stirring
Axle is mixed, one end of the agitating shaft is provided with motor.Due to needing the reagent used to have Taq when preparing gene detecting kit
Enzyme, primer, probe and dNTP etc. are the materials of Cord blood, therefore when preparing gene detecting kit, in order to keep most
Good effect, is prepared best at low ambient temperatures.Cooling water can directly be added in the cooling duct in cooling duct, then
Refresh the water periodically, cold water pipe can also be set to be passed through flow-type cold water in cold water pipe, cold water pipe can also be set simultaneously and in institute
Addition water in cooling duct is stated, now need not then change the water in the cooling duct, it is only necessary to circulate the cold water pipe
In water.The agitating shaft needs stirring at low speed to avoid producing bubble, and agitating shaft described in the motor driven is gently agitated for
Tens seconds.It is described to be fixed on the bracket with liquid bucket, it can also be provided that detachable.
Preferably, the cold water pipe for being used for being passed through cold water is provided with the cooling duct, the cold water pipe traverses whole cooling
Passage.Water in the cold water pipe can recycle, and facilitate temperature control.
Preferably, the cold water pipe is provided with flow control valve.The uninterrupted of cold water can be adjusted so as to preferably control
The environment temperature with liquid bucket.Temperature sensor can also be provided with the bracket in addition, can be passed according to the temperature
The uninterrupted of the temperature display control cold water of sensor.
Preferably, moveable ultrapure waterpipe is provided with the upper end open with liquid bucket, on the ultrapure waterpipe
Provided with flow regulator.Need to move the ultrapure waterpipe when adding ultra-pure water to the upper end open for matching somebody with somebody liquid bucket, according to reality
Border water requirement adds required water, and the flow regulator can be accurately controlled the amount of added ultra-pure water.
Preferably, described to be provided with proportioning device with liquid bottom of the barrel, the proportioning device is located on the support.It is described quantitative
Device can accurate quantification kit working solution gross mass, it is only necessary to the final densities of working solution are known, even if need not
The flow regulator can also accurately match somebody with somebody liquid, if flow regulator can be with the concentration of double control working solution.
Preferably, described to be provided with lid with liquid bucket, the lid is provided with liquid filling hole, and one end of the agitating shaft is through described
Lid connects the motor.The lid can prevent the thing on hair clothing from dropping and polluting working solution.
Preferably, the lid is made up of transparent materials such as browns, can prevent the fluorescence group in probe from degrading.
Preferably, the agitating shaft has retractility.It can be stirred according to the appropriate regulation of the depth with liquid bucket
Mix the length of axle.
Preferably, it is described to be movably arranged at liquid bucket on the support.It will can be shifted out with liquid bucket easy to clean simple.
The beneficial effects of the utility model are：Low temperature environment prepares gene detecting kit working solution, keeps working solution
Maximum activity, while the agitating device provided with only agitating shaft avoids stirring generation bubble from making Taq enzyme partial inactivation.
Brief description of the drawings
Accompanying drawing is used for providing further understanding to of the present utility model, and a part for constitution instruction, with this practicality
New embodiment is used to explain the utility model together, does not form to limitation of the present utility model.In the accompanying drawings：
Fig. 1 is the utility model structure diagram；
Wherein in figure mark for：1st, tank body；11st, tank skin；2nd, cooling duct；21st, cold water pipe；211st, flow control valve；3、
Support；4th, with liquid bucket；5th, lid；51st, liquid filling hole；6th, agitating shaft；7th, motor；8th, ultrapure waterpipe；9th, flow regulator；10、
Proportioning device.
Embodiment
As shown in figure 1, a kind of gene detecting kit liquid dispensing device, including tank body 1, tank body 1 is by two layers of 11 groups of tank skin
Into formation cooling duct 2 between tank skin 11, the inside of tank body 1 is provided with support 3, is movably equipped with support 3 with liquid bucket 4, with liquid bucket 4
Provided with opaque lid 5, lid 5 is provided with liquid filling hole 51, interior provided with the agitating shaft 6 with scalability, agitating shaft with liquid bucket 4
6 are provided with motor 7 through one end of lid 5.
Specifically, being provided with the cold water pipe 21 for being used for being passed through cold water in cooling duct 2, cold water pipe 21 traverses whole cooling duct
2.Cold water pipe 21 is provided with flow control valve 211.Water in cold water pipe 21 can recycle, and facilitate temperature control.By controlling flow
Regulating valve 211 controls the flow of cold water so as to controlling the environment temperature with liquid bucket 4.
Specifically, being provided with moveable ultrapure waterpipe 8 at the upper end open with liquid bucket 4, ultrapure waterpipe 8 is provided with stream
Measure adjuster 9.Ultra-pure water pipeline 8 is moved when needing to add ultra-pure water and arrives the upper end open with liquid bucket 4, and institute is added according to actual water requirement
Water is needed, flow regulator 9 can be accurately controlled the amount of added ultra-pure water.
Specifically, being provided with proportioning device 10 with the bottom of liquid bucket 4, proportioning device 10 is located on support 3.Proportioning device 10 can be with
The gross mass of accurate quantification kit working solution, it is only necessary to the final densities of working solution are known, even if not needing Flow-rate adjustment
Device 9 can also accurately match somebody with somebody liquid, if flow regulator 9 can be with the concentration of double control working solution.
Principle：
Due to needing the reagent used to there is Taq enzyme, primer, probe and dNTP etc. to be all when preparing gene detecting kit
The material of Cord blood, therefore when preparing gene detecting kit, in order to keep best effect, prepare at low ambient temperatures
It is best.Into cold water pipe 21, circulation is passed through cold water and keeps the low temperature environment with liquid bucket 4.Because high-speed stirred can make enzyme degrade, stir
Mixing axle 6 needs stirring at low speed to avoid producing bubble, and motor 7 drives agitating shaft 6 to be gently agitated for tens seconds.
Operating method：
The materials such as primer, probe and dNTP are sequentially added at the liquid filling hole 51 of the lid 5 with liquid bucket 4, add big portion
The ultra-pure water divided, is eventually adding Taq enzyme, then supplies final ultra-pure water according to the display of proportioning device.
The advantages of the present embodiment：
1st, the working solution of gene detecting kit, and stirring at low speed are prepared under low temperature environment, ensures that the maximum of working solution is living
Property；
The 2nd, opaque lid 5 is set, it is possible to reduce the degraded of the fluorescence group in probe；
3rd, it is movably equipped with liquid bucket 4, the convenient cleaning with liquid bucket 4；
4th, provided with flow regulator 9, the amount of water of ultra-pure water can accurately be controlled；
5th, provided with proportioning device 10, the concentration of working solution can be accurately controlled, reduces differences between batches.
Preferred embodiment of the present utility model is the foregoing is only, is not limited to the utility model, although ginseng
The utility model is described in detail according to previous embodiment, for those skilled in the art, it still can be with
Technical scheme described in foregoing embodiments is modified, or equivalent substitution is carried out to which part technical characteristic.It is all
Within the spirit and principles of the utility model, any modification, equivalent substitution and improvements made etc., this practicality should be included in
Within new protection domain.
Claims (8)
1. a kind of gene detecting kit liquid dispensing device, it is characterised in that including tank body, the tank body is by two layers of tank skin group
Into forming cooling duct between the tank skin, the tank interior is provided with support, and the support, which is provided with, matches somebody with somebody liquid bucket, described to match somebody with somebody
Agitating shaft is provided with liquid bucket, one end of the agitating shaft is provided with motor.
2. gene detecting kit liquid dispensing device according to claim 1, it is characterised in that set in the cooling duct
There is the cold water pipe for being passed through cold water, the cold water pipe traverses whole cooling duct.
3. gene detecting kit liquid dispensing device according to claim 2, it is characterised in that the cold water pipe is provided with stream
Adjustable valve.
4. gene detecting kit liquid dispensing device according to claim 1, it is characterised in that the upper end with liquid bucket
Opening is provided with moveable ultrapure waterpipe, and the ultrapure waterpipe is provided with flow regulator.
5. the gene detecting kit liquid dispensing device according to claim 1 or 4, it is characterised in that described to match somebody with somebody liquid bucket bottom
Portion is provided with proportioning device, and the proportioning device is located on the support.
6. gene detecting kit liquid dispensing device according to claim 1, it is characterised in that described to be provided with lid with liquid bucket
Body, the lid are provided with liquid filling hole, and one end of the agitating shaft connects the motor through the lid.
7. gene detecting kit liquid dispensing device according to claim 1, it is characterised in that the agitating shaft, which has, to be stretched
Contracting.
8. gene detecting kit liquid dispensing device according to claim 1, it is characterised in that described to be set with liquid bucket activity
In on the support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720834259.5U CN207062285U (en) | 2017-07-11 | 2017-07-11 | A kind of gene detecting kit liquid dispensing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720834259.5U CN207062285U (en) | 2017-07-11 | 2017-07-11 | A kind of gene detecting kit liquid dispensing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207062285U true CN207062285U (en) | 2018-03-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720834259.5U Active CN207062285U (en) | 2017-07-11 | 2017-07-11 | A kind of gene detecting kit liquid dispensing device |
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| CN (1) | CN207062285U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195017A (en) * | 2019-06-11 | 2019-09-03 | 郑州大学第二附属医院 | A kind of Parkinson's disease Disease-causing gene Mutation Screening detection method |
-
2017
- 2017-07-11 CN CN201720834259.5U patent/CN207062285U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195017A (en) * | 2019-06-11 | 2019-09-03 | 郑州大学第二附属医院 | A kind of Parkinson's disease Disease-causing gene Mutation Screening detection method |
| CN110195017B (en) * | 2019-06-11 | 2022-04-15 | 郑州大学第二附属医院 | Parkinson disease pathogenic gene mutation screening and detecting method |
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