CN207050849U - A kind of multiband fluorescence detection means in double fluorescent emission faces - Google Patents
A kind of multiband fluorescence detection means in double fluorescent emission faces Download PDFInfo
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- CN207050849U CN207050849U CN201720484767.5U CN201720484767U CN207050849U CN 207050849 U CN207050849 U CN 207050849U CN 201720484767 U CN201720484767 U CN 201720484767U CN 207050849 U CN207050849 U CN 207050849U
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- dichroic mirror
- collector lens
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Abstract
A kind of multiband fluorescence detection means in double fluorescent emission faces, it belongs to the technical field of spectral detection.The device includes main optical path, cuvette and two parallel legs, parallel legs and is mutually perpendicular to main optical path.A wave band light path in the device can detect the fluorescence intensity of internal standard substance, realize that Ratio-type detects, and have the advantages of detection operation is convenient, repeatability and accuracy are high.The fluorescence detection device, it is that increase fluorescence convergent lens and dichroic mirror are realized in two waveband fluorescence intensity detecting system.Compared with the multiband fluorescence detection means in single fluorescent emission face, dichroic mirror is reduced in the system structure quantity is set, make fluorescence loss less, light path it is short, there is higher sensitivity and lower detectable limit, available for it is quantitative detect trace materials portable fluorescence detector device.
Description
Technical field
A kind of multiband fluorescence detection means in double fluorescent emission faces is the utility model is related to, it belongs to the skill of spectral detection
Art field.
Background technology
Fluorescence intensity and detected material phase as internal standard compound transmitting can be detected simultaneously by the use of double light path fluorescence detecting system
The fluorescence intensity of association, realize that Ratio-type detects, reduce systematic error, improve accuracy rate and repeatability.A kind of patent " small capacity double
Light path fluorescence detection device, 201620412985.3 ", the aggregated lens of fluorescence that cuvette one side is launched are converted into parallel
Light, then it is divided into through dichroic mirror the fluorescence of two different-wavebands, finally reach photodiode via optical filtering and collector lens.This
In individual device from can low-voltage start, inexpensive general photodiode, realize small-sized Ratio-type fluoroscopic examination.Due to this
The dichroic mirror loss part fluorescence intensity that device is set, and light path is longer, and general photodiode light sensitivity is low, makes detection pole
Limit is higher, it is difficult to reaches the detectable limit of trace materials.In addition, double light path fluorescence detection device can not realize more than 2 differences
Fluorescence emission wavelengths are detected, and detection project is restricted.
The content of the invention
To solve problems of the prior art, the utility model provides a kind of multiband fluorescence in double fluorescent emission faces
Detecting system, on the basis of the advantages of keeping the two waveband Ratio-type detection in single fluorescent emission face, further improve the spirit of instrument
Detected while sensitivity, reducing detectable limit, and realize more fluorescence bands.Two waveband fluorescence intensity detecting system, it is by with swashing
Fluorescence is assembled in the perpendicular cuvette both sides of luminous road respectively, and is realized by the optical filtering of different-waveband；Three wave bands and four ripples
Section fluorescence intensity detecting system, it is to increase fluorescence convergent lens and dichroic mirror realization in two waveband fluorescence intensity detecting system
's.
The technical solution adopted in the utility model is：A kind of multiband fluorescence detection means in double fluorescent emission faces, it is wrapped
Housing is included, the device also includes main optical path, fluorescence cuvette, the first parallel legs and the second parallel legs, main optical path and first
Parallel legs are mutually perpendicular to, and main optical path and the second parallel legs are orthogonal, and the first parallel legs, fluorescence cuvette and second are flat
Row branch road is on same straight line；The main optical path uses light source, lamp condenser lens and light source filter；First parallel legs
Include the first fluorescence collector lens, the first fluorescence optical filtering and the first photodiode, the second parallel legs successively includes successively
Second fluorescence collector lens, the second fluorescence optical filtering and the second photodiode.
Further, the device, which is additionally included between fluorescence cuvette and the first parallel legs, sets gradually the first fluorescence optically focused
Lens and the first dichroic mirror, set and the first perpendicular vertical leg of the first parallel legs on the first dichroic mirror；Described first
Vertical leg sets gradually the 3rd fluorescence collector lens, the 3rd fluorescence optical filtering and the 3rd photodiode from the first dichroic mirror.
Further, the device, which is additionally included between fluorescence cuvette and the second parallel legs, sets gradually the second fluorescence optically focused
Lens and the second dichroic mirror, set and the second perpendicular vertical leg of the second parallel legs on the second dichroic mirror；Described second
Vertical leg sets gradually the 4th fluorescence collector lens, the 4th fluorescence optical filtering and the 4th photodiode from the second dichroic mirror.
The light source is selected from LED or tungsten lamp.
The beneficial effect of the application is：It is parallel with second that the device includes main optical path, fluorescence cuvette, the first parallel legs
Branch road, main optical path are mutually perpendicular to the first parallel legs, and main optical path and the second parallel legs are orthogonal, the first parallel legs,
Fluorescence cuvette and the second parallel legs are on same straight line.Separately dichroic mirror, two colors are set between cuvette and parallel legs
Jing Chu sets vertical leg.It is used to detect the optics of fluorescent dye transmitting fluorescence intensity change in liquid based on fluorescence spectroscopy technique
System, system include excitation source, fluorescence filter and fluorescence intensity detection three parts.In multiband fluorescence detection optical system
One wave band light path can detect the fluorescence intensity of internal standard substance, realize that Ratio-type detects, have convenient detection operation, repeatability and
The advantages of accuracy is high.Compared with the multiband fluorescence intensity detection system in single fluorescent emission face, due in the system structure
Reduce dichroic mirror and quantity be set, make fluorescence loss less, light path it is short, photoelectric transformation efficiency is high, improves detection sensitivity and reduces and examines
The limit is surveyed, light channel structure is compacter, assembling facility, available for smaller portable fluorescence detector device.
Brief description of the drawings
Fig. 1 is a kind of three wave band fluorescence detection device schematic diagrames in double fluorescent emission faces.
Fig. 2 is a kind of four wave band fluorescence detection device schematic diagrames in double fluorescent emission faces.
In figure：1st, light source, 2, lamp condenser lens；2a, the first fluorescence collector lens, 2b, the second fluorescence collector lens,
2c, the 3rd fluorescence collector lens, 2d, the 4th fluorescence collector lens, 3, light source filter, 3a, the first fluorescence optical filtering, 3b,
Two fluorescence optical filterings, 3c, the 3rd fluorescence optical filtering, 3d, the 4th fluorescence optical filtering, 4, fluorescence cuvette, 5a, the pole of the first photoelectricity two
Pipe, 5b, the second photodiode, 5c, the 3rd photodiode, 5d, the 4th photodiode, 6a, the first fluorescence convergent lens,
6b, the second fluorescence convergent lens, 7a, the first dichroic mirror, 7b, the second dichroic mirror.
Embodiment
Embodiment 1
Fig. 1 shows a kind of three wave band fluorescence detection optical path systems in double fluorescent emission faces, including main optical path, fluorescence colorimetric
Ware 4, the first parallel legs and the second parallel legs, main optical path are mutually perpendicular to the first parallel legs, and main optical path is parallel with second
Branch road is orthogonal, and the first parallel legs, the parallel legs of fluorescence cuvette 4 and second are on same straight line；Main optical path uses
Light source 1, lamp condenser lens 2 and light source filter 3；First parallel legs include the first fluorescence collector lens 2a, first successively
Fluorescence optical filtering 3a and the first photodiode 5a, the second parallel legs include the second fluorescence collector lens 2b, second glimmering successively
Light optical filtering 3b and the second photodiode 5b.It is additionally included between the parallel legs of fluorescence cuvette 4 and first and sets gradually
First perpendicular with the first parallel legs is set to hang down on one fluorescence collector lens 6a and the first dichroic mirror 7a, the first dichroic mirror 7a
Allotment road；First vertical leg is from the first dichroic mirror 7a sets gradually the 3rd fluorescence collector lens 2c, the 3rd fluorescence filters
Mirror 3c and the 3rd photodiode 5c.
The fluorescence of b wave band light paths is come out from a cuvette surface launching perpendicular to excitation light path, then by second
Fluorescence collector lens 2b, the second fluorescence optical filtering 3b, finally reach the second photodiode 5b and are converted into electric signal；With b ripples
Section is corresponding, and the fluorescence for another cuvette surface launching being parallel to each other, through the first fluorescence convergent lens 6a and the one or two color
It is two wave band fluorescence of a and c that 7a, which is divided to,；A wave bands fluorescence passes through the first fluorescence collector lens 2a, the first fluorescence optical filtering 3a again, most
After reach the first photodiode 5a and be converted into electric signal；Wave band c fluorescence is again by the 3rd fluorescence collector lens 2c, the 3rd glimmering
Light optical filtering 3c, finally reach the 3rd photodiode 5c and be converted into electric signal.
Embodiment 2
Fig. 2 shows a kind of four wave band fluorescence detection optical path systems in double fluorescent emission faces, including main optical path, fluorescence colorimetric
Ware 4, the first parallel legs and the second parallel legs, main optical path are mutually perpendicular to the first parallel legs, and main optical path is parallel with second
Branch road is orthogonal, and the first parallel legs, the parallel legs of fluorescence cuvette 4 and second are on same straight line；Main optical path uses
Light source 1, lamp condenser lens 2 and light source filter 3；First parallel legs include the first fluorescence collector lens 2a, first successively
Fluorescence optical filtering 3a and the first photodiode 5a, the second parallel legs include the second fluorescence collector lens 2b, second glimmering successively
Light optical filtering 3b and the second photodiode 5b.It is additionally included between the parallel legs of fluorescence cuvette 4 and first and sets gradually
First perpendicular with the first parallel legs is set to hang down on one fluorescence collector lens 6a and the first dichroic mirror 7a, the first dichroic mirror 7a
Allotment road；First vertical leg is from the first dichroic mirror 7a sets gradually the 3rd fluorescence collector lens 2c, the 3rd fluorescence filters
Mirror 3c and the 3rd photodiode 5c.It is saturating that the second fluorescence optically focused is set gradually between the parallel legs of fluorescence cuvette 4 and second
Set and the second perpendicular vertical leg of the second parallel legs on mirror 6b and the second dichroic mirror 7b, the second dichroic mirror 7b；It is described
Second vertical leg sets gradually the 4th fluorescence collector lens 2d, the 4th fluorescence optical filtering 3d and the 4th light from the second dichroic mirror 7b
Electric diode 5d.
Go out two-way fluorescence from two cuvette surface launchings being parallel to each other perpendicular to excitation light path, wherein fluorescence passes through all the way
It is two wave band fluorescence of a and c that first fluorescence convergent lens 6a and the first dichroic mirror 7a, which is divided to,；A wave bands fluorescence passes through the first fluorescence again
Collector lens 2a, the first fluorescence optical filtering 3a, finally reach the first photodiode 5a and are converted into electric signal；Wave band c fluorescence
Pass through the 3rd fluorescence collector lens 2c, the 3rd fluorescence optical filtering 3c again, finally reach the 3rd photodiode 5c and be converted into electricity
Signal；It is two wave band fluorescence of b and d that another way fluorescence is divided to through the second fluorescence convergent lens 6b and the second dichroic mirror 7b；B wave bands are glimmering
Light passes through the second fluorescence collector lens 2b, the second fluorescence optical filtering 3b again, finally reaches the second photodiode 5b and is converted into
Electric signal；D wave bands fluorescence passes through the 3rd fluorescence collector lens 2d, the 3rd fluorescence optical filtering 3d again, finally reaches the 3rd photoelectricity two
Pole pipe 5d is converted into electric signal.
Embodiment 3
In embodiment 1,2, one of wave band as internal standard fluorescent dye intensity detect, with its all band fluorescence intensity with
The ratio conversion detected material content of this internal standard fluorescence intensity, realizes that Ratio-type detects.
It is saturating by fluorescence convergent lens and dichroic beam splitter and optical filtering and fluorescence optically focused from the fluorescence of cuvette transmitting
When mirror reaches photodiode, due to the loss of optical length and dichroic beam splitter, the sensitivity of detection decreases, and is unable to reach
Than the project demand of relatively low detectable limit.For the two waveband light path detection in the application, because not setting dichroic beam splitter,
Detected from which light path of a and b as low detectable limit material, be all up requiring；, can for three wave band light path detections
Detected from b roads as relatively low detectable limit material, the detection of reference internal standard dye fluorescence, another way are elected as one in other two-way
Detected as compared with high detection limit material.
Claims (3)
1. a kind of multiband fluorescence detection means in double fluorescent emission faces, it includes housing, it is characterised in that：The device also includes
Main optical path, fluorescence cuvette（4）, the first parallel legs and the second parallel legs, main optical path be mutually perpendicular to the first parallel legs,
Main optical path and the second parallel legs are orthogonal, the first parallel legs, fluorescence cuvette（4）It is in same with the second parallel legs
On straight line；The main optical path uses light source（1）, lamp condenser lens（2）And light source filter（3）；First parallel legs are successively
Including the first fluorescence collector lens（2a）, the first fluorescence optical filtering（3a）With the first photodiode（5a）, the second parallel legs
Include the second fluorescence collector lens successively（2b）, the second fluorescence optical filtering（3b）With the second photodiode（5b）；The device is also
It is included in fluorescence cuvette（4）The first fluorescence collector lens is set gradually between the first parallel legs（6a）With the first dichroic mirror
（7a）, the first dichroic mirror（7a）Upper setting and the first perpendicular vertical leg of the first parallel legs；First vertical leg
From the first dichroic mirror（7a）Set gradually the 3rd fluorescence collector lens（2c）, the 3rd fluorescence optical filtering（3c）With the pole of the 3rd photoelectricity two
Pipe（5c）.
A kind of 2. multiband fluorescence detection means in double fluorescent emission faces according to claim 1, it is characterised in that：The dress
Put and be additionally included in fluorescence cuvette（4）The second fluorescence collector lens is set gradually between the second parallel legs（6b）With the two or two
Look mirror（7b）, the second dichroic mirror（7b）Upper setting and the second perpendicular vertical leg of the second parallel legs；Described second is vertical
Branch road is from the second dichroic mirror（7b）Set gradually the 4th fluorescence collector lens（2d）, the 4th fluorescence optical filtering（3d）With the 4th photoelectricity
Diode（5d）.
A kind of 3. multiband fluorescence detection means in double fluorescent emission faces according to claim 1, it is characterised in that：It is described
Light source（1）Selected from LED or tungsten lamp.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720484767.5U CN207050849U (en) | 2017-05-04 | 2017-05-04 | A kind of multiband fluorescence detection means in double fluorescent emission faces |
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| CN201720484767.5U CN207050849U (en) | 2017-05-04 | 2017-05-04 | A kind of multiband fluorescence detection means in double fluorescent emission faces |
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Effective date of registration: 20210926 Address after: 201500 room 121, floor 1, Lane 11, Puwei Road, Shanyang Town, Jinshan District, Shanghai Q3 Patentee after: Shanghai mingluo Technology Co.,Ltd. Address before: 210000 room B503, building 2, Guorui building, No. 359, Jiangdong Middle Road, Jianye District, Nanjing, Jiangsu Province Patentee before: Cui Jingnan Patentee before: Wang Zheng |