CN207020195U - A kind of effluent piece for detecting lean meat Compounds - Google Patents
A kind of effluent piece for detecting lean meat Compounds Download PDFInfo
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- CN207020195U CN207020195U CN201720857300.0U CN201720857300U CN207020195U CN 207020195 U CN207020195 U CN 207020195U CN 201720857300 U CN201720857300 U CN 201720857300U CN 207020195 U CN207020195 U CN 207020195U
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Abstract
The utility model discloses a kind of effluent piece for detecting lean meat Compounds, the effluent piece includes a detection and blocked, described detection card includes backing, and sample pad on backing, gold standard pad, nitrocellulose filter, the first detection zone and the second detection zone, quality control region and absorption pad.Effluent piece of the present utility model can detect a variety of clenbuterol hydrochloride class compounds simultaneously, suitable for the quick detection of clenbuterol hydrochloride class.
Description
Technical field
Detection inspection technology field is the utility model is related to, more particularly to a kind of effluent for detecting lean meat Compounds
Piece.
Background technology
Because the modernization development of animal husbandry using antibiotic, hormone and other synthetic drugs, it is necessary to largely carry out diseases prevention
Cure the disease, the problems such as so as to cause medicament residue in animal foods, hormone residues, health and life are constituted a threat to and endangered
Evil.It is a kind of clenbuterol hydrochloride such as clenobuterol hydrochloride (Clenbuterol), is a kind of artificial synthesized adrenaline god
Through excitant.Dosage is exceeded to cause the symptoms such as human body produces palpitaition, vomiting, muscle do not tremble independently, or even is in peril of one's life.
If dosage increases to 5-10 times of therapeutic dose, there is the fat redistribution for making trunk, promote protein synthesis and promote growth
Effect, so be used as additive for farm animal feed, to reduce content of fat in body, improve lean meat percentage, promote growth, people is eating
With remained containing clenbuterol hydrochloride livestock meat, egg, milk deli when, food poisoning easily occurs.
The common method of detection clenbuterol hydrochloride is including chromatographic technique etc. at present.Although chromatographic technique is effective, measuring samples need
It is cumbersome time-consuming through a series of pretreatment, from sample pretreatment to showing that testing result at least needs 2 day time；It is this in addition
Detection method also needs to expensive instrument and equipment, and needs professional to operate, and can only detect a sample every time, serious resistance
Hinder the promotion and popularization of chromatographic technique, can not more realize Site Detection.
Therefore, good stability, high sensitivity, quickly specific detection lean meat Compounds easy to use are badly in need of in this area
Effluent piece.
Utility model content
The purpose of this utility model is to provide a kind of good stability, high sensitivity, quick specific detection easy to use
The effluent piece of lean meat Compounds.
In first aspect of the present utility model, there is provided a kind of effluent piece for detecting lean meat Compounds, the effluent piece
Including a detection card, described detection card includes backing (8), and the following knot for proximally arriving distal end on the backing
Structure：Sample pad (1), gold standard pad (2), nitrocellulose filter (6), detection zone, quality control region (5) and absorption pad (7)；
And described gold standard pad (2) includes：First antibody load region and secondary antibody load region, wherein, described
One antibody is directed to the husky fourth antibody of first kind lean meat Compounds for the specificity of colloid gold label, and described secondary antibody is glue
The specificity of body gold mark is directed to the Rec antibody of the second class lean meat Compounds；
And the detection zone includes：First detection zone (3) and the second detection zone (4), wherein, the first described detection zone
(3) comlete antigen of first kind lean meat Compounds is coated with, described the second detection zone (4) is coated with the second class lean meat and refined
The comlete antigen of compound；
And described first kind lean meat Compounds are selected from the group：Clenbuterol, salbutamol, bambuterol, western Boot
Sieve, Mabuterol, bromine Boot sieve, Sigma spy sieve, Clorprenaline or its combination；
And the second described class lean meat Compounds are Ractopamine.
In another preference, the comlete antigen of described first kind lean meat Compounds is first kind lean meat Compounds
With the conjugate of carrier protein.
In another preference, the comlete antigen of the second described class lean meat Compounds is the second class lean meat Compounds
With the conjugate of carrier protein.
In another preference, the carrier protein is selected from the group：BSA, KLH, OVA or its combination.
In another preference, described backing (8) is PVC board.
In another preference, the sample pad (1) is glass fibre.
In another preference, the absorption pad (7) is blotting paper.
In another preference, described sample pad (1), gold standard pad (2), nitrocellulose filter (6) and absorption pad (7)
It is pasted on the backing.
In another preference, described sample pad (1) and gold standard pad (2) partly overlap.
In another preference, described the first detection zone (3) and the second detection zone (4) are positioned close to gold standard pad (2)
Side, and described quality control region (5) is positioned close to the side of absorption pad (7).
In another preference, the effluent piece also includes cartridge (9), and the well in the cartridge
And observation window (11) (10).
In another preference, described detection card is completely set up in cartridge (9), and the well (10) corresponds to institute
The sample pad (1) on detection card is stated, and the observation window (11) corresponds to first detection zone (3) detected on card, second
Detection zone (4) and quality control region (5).
In another preference, the length of the cartridge is 80-90.0mm, width 10-20mm, and height is 4-5mm.
In another preference, the length of the effluent piece is 60-70mm, and width is 3-4mm.
In another preference, the quality control region (5) includes the 3rd antibody, and the 3rd antibody specificity is incorporated into described
First antibody and secondary antibody.
In another preference, described the first detection zone (3), the second detection zone (4) and quality control region (5) are located at nitric acid fibre
Tie up on plain film (6).
In another preference, the sample of the effluent piece detection includes whole blood, blood plasma, serum, urine or tissue sample
This.
In second aspect of the present utility model, there is provided a kind of kit for being used to detect clenbuterol hydrochloride, the kit bag
Include：
(i) container；And
(ii) the effluent piece of the detection lean meat Compounds as described in the utility model first aspect.
In another preference, the kit also includes specification.
It should be understood that in the scope of the utility model, above-mentioned each technical characteristic of the present utility model and below (such as implementation
Example) in specifically describe each technical characteristic between can be combined with each other, so as to form new or preferable technical scheme.It is limited to
Length, no longer tire out one by one state herein.
Brief description of the drawings
Fig. 1 is a kind of structure sectional view of the detection card of preference of the utility model.Wherein, 1 is sample pad, and 2 be gold mark
Pad, 3 be the first detection zone, and 4 be the second detection zone, and 5 be quality control region, and 6 be nitrocellulose filter, and 7 be absorption pad, and 8 be backing.
Fig. 2 is a kind of top view of the effluent piece of the detection lean meat Compounds of preference of the utility model.Wherein, 9 are
Cartridge, 10 be well, and 11 be observation window.
Embodiment
The present inventor by depth studying extensively, it has unexpectedly been found that a kind of effluent for detecting lean meat Compounds
Piece, the effluent piece can a variety of lean meat Compounds of specific detection, including but not limited to clenobuterol hydrochloride, salbutamol, class
Boot sieve, western Boot sieve, Mabuterol, bromine Boot sieve, Sigma spy sieve, Clorprenaline, Ractopamine.On this basis, it is complete
Into the utility model.
Lean meat Compounds
Clenbuterol hydrochloride is the general designation of a kind of medicine, mainly adrenal gland class, beta-agonists, beta-stimulants, for treating bronchus
The diseases such as asthma, chronic bronchitis and pulmonary emphysema.Heavy dose is used in the growth that can promote pig in feed, reduces fat and contains
Amount, lean meat percentage is improved, but the edible pork containing clenbuterol hydrochloride is harmful.
Currently used lean meat Compounds mainly have following several：
Clenbuterol (clenobuterol hydrochloride), salbutamol, bambuterol, western Boot sieve, Mabuterol, bromine Boot sieve,
Sigma spy sieve, Clorprenaline, Ractopamine.
Effluent piece disclosed in the utility model can detect a variety of lean meat Compounds simultaneously, specifically, according to detection when
The difference of colour developing position, can include first kind lean meat Compounds (Clenbuterol, salbutamol, bambuterol, western Boot
Sieve, Mabuterol, bromine Boot sieve, Sigma spy sieve, Clorprenaline) and the second class lean meat Compounds be Ractopamine.
Detection method and operation principle
For the ease of understanding the utility model, the Cleaning Principle of the utility model effluent piece is provided.It should be understood that this practicality is new
The protection domain of type is not influenceed or limitation by the principle.
The utility model uses the principle of competition law immunochromatography, is co-owned in the structure of first kind lean meat Compounds
N tert-butyl group classes phenyl ethylamine forms, so the antibody that immunoscreening obtains can identify this similar position jointly.By first kind clenbuterol hydrochloride
(i.e. first kind lean meat Compounds is completely anti-for the first compound that a certain kind in compound is formed with carrier protein couplet
It is former) and the second class lean meat Compounds and carrier protein couplet formation the second compound (i.e. the second class lean meat Compounds
Comlete antigen) the first detection zone and the second detection zone (solid phase antigen) that are individually fixed on nitrocellulose membrane, by the anti-first kind
The colloid gold label thing of the antibody colloidal gold label of clenbuterol hydrochloride and anti-second class clenbuterol hydrochloride antibody, detection strip is formed by Fig. 1
And in being loaded.After sample to be checked is added in the sample pad of detection card one end by well, moved forward by capillarity,
Dissolving combines the first antibody and secondary antibody of the colloid gold label in gold standard pad, then is moved to the first detection zone, second successively
Detection zone, quality control region.If not containing any lean meat Compounds in sample, the first antibody and secondary antibody of colloid gold label
The first compound that can be fixed on respectively on nitrocellulose filter and the capture of the second compound, so as in the first detection zone and the
Two detection zones form red stripes.If such as contain hydrochloric acid Ke Lunte containing certain lean meat Compounds of finite concentration in sample
Sieve, the first antibody of colloid gold label can be combined first after being dissolved with clenobuterol hydrochloride in sample in gold standard pad, so as to press down
The combination of the first compound on first antibody and nitrocellulose filter processed, the band color of the first detection zone will weaken or
Disappear；The combination of secondary antibody and the second compound is unaffected, and the second detection zone still forms red stripes.In sample
Contain two class lean meat Compounds, first antibody and secondary antibody and first on nitrocellulose filter of colloid gold label simultaneously
The combination of compound and the second compound can be suppressed, and the band color of the first detection zone and the second detection zone can weaken
Or disappear.
In other words, when containing lean meat Compounds in sample to be checked, the first detection zone and the second detection zone are non-discolouring,
When containing the compound that is selected from the group in sample to be checked：Clenobuterol hydrochloride, salbutamol, bambuterol, western Boot sieve, Ma Bu
Special sieve, bromine Boot sieve, Sigma spy sieve, Clorprenaline or its combination, then the first detection zone color disappears or color substantially weakens.
When containing Ractopamine in sample to be checked, then the second detection zone color disappears or color substantially weakens.It can be seen by naked eyes
Observe colour developing result.
The utility model is provided with the 3rd antibody in the quality control region of nitrocellulose filter, and the 3rd antibody specificity combines
In the first antibody and secondary antibody, no matter whether contain lean meat Compounds in measuring samples, the first of colloid gold label
Antibody and secondary antibody can form red stripes with the 3rd antibody binding of quality control region, and the band is whether to judge chromatography process
The standard whether normal and detection plate fails, while also serve as controlling the parameter of difference between batch in quantitative analysis.
Sensitivity
Effluent piece sensitivity of the present utility model is as follows, clenobuterol hydrochloride, salbutamol, bambuterol, western Boot sieve,
The sensitivity of Mabuterol, bromine Boot sieve, Sigma spy sieve is 3ng/ml, and the sensitivity of Clorprenaline is 8ng/ml.Rec DOPA
The sensitivity of amine is 3ng/ml.
The manufacture of effluent piece
In the utility model, the existing material system in this area can be selected in each element (or component) of the effluent piece
Into.
Backing 8 can be made of any stabilization, non-porous material, and its intensity should be enough supporting material and adhere thereto
Each element.Because many measure are by the use of water as dispersive medium, therefore backing 8 is preferably substantially water-impervious.One
In individual preference, backing 8 is made of polymer film, is used made of polychloroethylene film (such as PVC offset plates).
Sample pad 1 can be made of any absorbent material.Workable example of material includes：Cellulose, nitrocellulose,
Cellulose acetate, glass fibre, nylon, polyelectrolyte ion exchange membrane, propylene copolymer/nylon and polyether sulfone.
Absorption pad 7 can be made of any material that can be absorbed as sample and the liquid of buffer solution.The suction of absorption pad 7
Receipts ability is sufficiently large, to absorb the liquid for being added to test-strips.Include cellulose suitable for the example of the material of absorption pad 7
And absorbent filter.
The preparation of antibody
By dosage of the husky fourth antigen coupling carrier albumen (BSA, OVA etc.) by every 50 μ g/ml and isometric Freund's adjuvant
4 6-8 week old male BALB/C mices are immunized in the fully emulsified subcutaneous multi-point injection of postabdomen, are immunized 7-10 days, tail vein blood,
Indirect ELISA detects serum titer.The preferable mouse spleen of immune effect is melted with myeloma cell SP2/0 by PEG mediations
Close, indirect ELISA screening positive clone；Positive colony obtains the monoclonal cell of inheritance stability after limiting dilution is subcloned
Strain, after cell line expands culture, it is inoculated with the ascites that production monoclonal antibody is induced through norphytane sensitization BALB/C mice abdominal cavity.Abdomen
Water slightly purifies through thiamines, then sad thiamines method purify corresponding desertification class D monoclonal antibody, be stored in 4 degree.
First kind lean meat Compounds (Clenbuterol, salbutamol, class's Boot are included in the structure of above-mentioned husky fourth antigen
Sieve, western Boot sieve, Mabuterol, bromine Boot sieve, Sigma spy sieve, Clorprenaline) common structure, therefore the above method prepare
Antibody can be combined with a variety of first kind lean meat Compounds simultaneously, be that specificity is directed to first kind lean meat Compounds the
One antibody.
Rec antibody used in the utility model is commercially available, and as specificity is directed to the second of the second class lean meat Compounds
Antibody.
For 3rd antibody in combination with first antibody and secondary antibody, preferable 3rd antibody is that sheep anti-mouse igg is more anti-.
Colloid gold label
Collaurum is to be acted on by gold chloride (HAuCl4) in reducing agent such as white phosphorus, ascorbic acid, sodium citrate, tannic acid etc.
Under, a certain size gold grain is can be grouped to, and because electrostatic interaction turns into a kind of colloidal state of stabilization, formed electronegative
Hydrophobic sol solution, turn into stable colloidal state due to electrostatic interaction, therefore claim collaurum.
Collaurum is negatively charged under mild alkaline conditions, can form firm combination with the positive charge group of protein molecule,
Because this combination is electrostatical binding, so not influenceing the biological nature of protein.Collaurum in addition to being combined with protein,
It can also be combined with many other large biological molecules, such as SPA, PHA, ConA.According to some physical behaviors of collaurum, such as
High electron density, granular size, shape and color reaction, plus the immune and biological characteristics of conjugate, thus make collaurum
It is widely used in the fields such as immunology, histology, pathology and cell biology.
The macromolecules such as colloid gold label, substantially protein are adsorbed to the coating process on colloid gold particle surface.Inhale
Random reason is probably colloid gold particle surface negative charge, and firm knot is formed because of Electrostatic Absorption with the positive charge group of protein
Close.The colloid gold particle of various different-grain diameters, namely different colours can be easily prepared from gold chloride with reducing process.It is this
Spherical particle has very strong adsorption function to protein, can be with staphylococcal protein A, immunoglobulin, toxin, sugared egg
In vain, the Non-covalent binding such as enzyme, antibiotic, hormone, bovine serum albumin polypeptide conjugate, thus in basic research and clinical trial
In turn into highly useful instrument.
Immuno-gold labeling technology (Immunogold labelling technique) mainly make use of gold grain to have height
The characteristic of electron density, marked in gold at protein binding, under the microscope visible dark brown coloured particles, when these labels are corresponding
When largely assembling at part, naked eyes red color visible or pink spot, thus detected for qualitative or sxemiquantitative tachysynthesis
In method, this reaction can also be exaggerated by the deposition of Argent grain, referred to as immunogold silver staining.
In the preparation of the utility model lateral flow strip, it is preferable that 40nm collaurums are taken, with 0.1M K2CO3PH is adjusted, with every milli
Rising the μ g antibody of collaurum 5 to be marked, must be precipitated after marking fluid centrifugation, precipitation 10mM pH7.2PBS dissolve, and in 650nm
Lower measure light absorption value OD, apply on paving polyester material and dry with 1-3OD, as the gold standard pad in the utility model detection card.
The preparation of lateral flow strip
Each element (or component) of the utility model effluent piece is using technical method manufacture generally in the art and group
Dress.
Preferably, the first antigen, the second antigen and Quality Control antibody are diluted with coating buffer solution, by three kinds of captures after dilution
Liquid is equably coated on the nitrocellulose membrane as detection part respectively, and three kinds of capture liquid divide after penetrating into nitrocellulose filter
The first detection zone, the second detection zone and quality control region are not formed.
Sample pad, gold standard pad, nitrocellulose filter, absorption pad are pasted on backing by adhesive respectively.With slitting
Machine-cut is placed in cartridge, well alignment detection card sample pad location into 4mm detection card, detection reagent is assembled into after compression
Box, add drier foil sealing packaging and preserve.
The advantages of the utility model：
The effluent piece of detection lean meat Compounds described in the utility model overcomes and needs to detect one by one not in existing method
The drawbacks of with kind clenbuterol hydrochloride, specific detection antibody can all be specifically bound to a variety of clenbuterol hydrochlorides, can be achieved to clenbuterol hydrochloride class
The detection for the quickly, efficiently and accurately whetheing there is.
Below in conjunction with specific embodiment, the utility model is further illustrated.It should be understood that following description is only this practicality
New most preferred embodiment, and it is not construed as the limitation for scope of protection of the utility model.Fully understanding
On the basis of the utility model, generally according to normal condition, or according to the condition proposed by manufacturer, those skilled in the art
Nonessential change can be made to the technical solution of the utility model, such change should be considered as being included in this practicality newly
Among the protection domain of type.
Embodiment 1
The structure of effluent piece
The effluent piece (or test-strips) of detection lean meat Compounds of the present utility model includes cartridge and in cartridge
Detection card.
A kind of structure of preferable detection card is as shown in figure 1, it includes backing 8, and on backing：Sample pad 1,
Gold standard pad 2, nitrocellulose filter 6, detection zone (or detection line, including the first detection zone 3 and the second detection zone 4, quality control region 5 (or
Check plot, control line, nature controlling line) and absorption pad 7.
A kind of structure of preferable cartridge 9 as shown in Fig. 2 include well 10 and observation window 11 thereon.It is also, described
Detection card is completely set up in cartridge 9, the sample pad 1 that the well 10 corresponds on the detection card, and the observation window
11 the first detection zone 3, the second detection zone 4 and the quality control regions 5 corresponded on the detection card
Specifically, effluent piece of the present utility model has the characteristics that：
The length of backing 8 is identical with effluent piece.
Sample pad 1 is located at one end of test-strips, and absorption pad 7 is located at the other end of test-strips.
Two kinds of antibody are loaded with gold standard pad 2, first antibody is directed to first kind clenbuterol hydrochloride for the specificity of colloid gold label
Compound (including Clenbuterol, salbutamol, bambuterol, western Boot sieve, Mabuterol, bromine Boot sieve, Sigma spy sieve, chlorine
Third that woods) husky fourth antibody；Described secondary antibody is directed to the second class lean meat Compounds Lay for the specificity of colloid gold label
The Rec antibody of gram dopamine, the antibody are commercially available.
Detection zone (also referred to as detection line) is between sample pad 1 and absorption pad 7, including the first detection zone 3 and the second detection
Area 4, it is preferable that the first detection zone 3 is located to be arranged on nitrocellulose filter 6 close to one end of sample pad 1, usual detection zone,
Pass through the gold standard pad 2 and adsorptive pads 7 of the nitrocellulose filter linkage flag.Preferably, on the nitrocellulose filter 6 also
Quality control region 5 (also referred to as nature controlling line) is provided with, quality control region 5 is between detection zone and absorption pad 7.
Wherein, the first described detection zone 3 be coated with that first kind lean meat Compounds and carrier protein couplet formed the
The comlete antigen of one compound, i.e. first kind lean meat Compounds；The second described detection zone 4 is coated with the second class lean meat and refined
The comlete antigen of the second compound, i.e. the second class lean meat Compounds that compound is formed with carrier protein couplet.Quality control region 5 is coated with
There is the 3rd antibody, the 3rd antibody specificity is incorporated into described first antibody and secondary antibody.
Embodiment 2
The use of effluent piece
1. acquisition testing sample, sample can be whole blood, blood plasma, serum, urine or tissue samples, and the present embodiment uses
Freshly voided urine is as detection sample；
2. detection card is kept flat, sample solution to be checked is drawn with dropper, vertical dropwise addition 3 is dripped in well, opened after sample-adding
Beginning timing.
Testing result is read in 3.5 minutes.
As a result judge：
A：First detection zone, the second detection zone and quality control region change colour (red) simultaneously, show to be free of lean meat in urine sample
The concentration of Compounds or lean meat Compounds is less than minimum detected value, and testing result is feminine gender.
B:Quality control region changes colour (red), and the first detection zone and the second detection zone are non-discolouring, show in urine sample simultaneously
Containing first kind lean meat Compounds and the second class lean meat Compounds, testing result is the positive.
C：Quality control region and the first detection zone discoloration (red), and the second detection zone is non-discolouring, shows to contain in urine sample
Second class lean meat Compounds, testing result are the positive.
D：Quality control region and the second detection zone discoloration (red), and the first detection zone is non-discolouring, shows to contain in urine sample
First kind lean meat Compounds, testing result are the positive.
E：Quality control region is non-discolouring, and no matter whether the first detection zone and the second detection zone change colour, and show that this detection is invalid.
All it is incorporated as referring in this application in all documents that the utility model refers to, just as each document quilt
It is individually recited as with reference to such.In addition, it is to be understood that after above-mentioned instruction content of the present utility model has been read, this area skill
Art personnel can make various changes or modifications to the utility model, and these equivalent form of values equally fall within the application appended claims
Book limited range.
Claims (10)
1. a kind of effluent piece for detecting lean meat Compounds, it is characterised in that the effluent piece includes a detection and blocked, described inspection
Surveying card includes backing (8), and the following structure for proximally arriving distal end on the backing：Sample pad (1), gold standard pad
(2), nitrocellulose filter (6), detection zone, quality control region (5) and absorption pad (7)；
And described gold standard pad (2) includes：First antibody load region and secondary antibody load region, wherein, described first is anti-
Body is directed to the husky fourth antibody of first kind lean meat Compounds for the specificity of colloid gold label, and described secondary antibody is collaurum
The specificity of mark is directed to the Rec antibody of the second class lean meat Compounds；
And the detection zone includes：First detection zone (3) and the second detection zone (4), wherein, described the first detection zone (3)
The comlete antigen of first kind lean meat Compounds is coated with, described the second detection zone (4) is coated with the second class clenbuterol hydrochloride chemical combination
The comlete antigen of thing；
And described first kind lean meat Compounds are selected from the group：Clenbuterol, salbutamol, bambuterol, western Boot sieve, horse
Boot sieve, bromine Boot sieve, Sigma spy sieve, Clorprenaline or its combination；
And the second described class lean meat Compounds are Ractopamine.
2. effluent piece as claimed in claim 1, it is characterised in that described the first detection zone (3) and the second detection zone (4) is set
Put in the side of close gold standard pad (2), and described quality control region (5) is positioned close to the side of absorption pad (7).
3. effluent piece as claimed in claim 1, it is characterised in that the effluent piece also includes cartridge (9), and positioned at described
Well (10) and observation window (11) in cartridge.
4. effluent piece as claimed in claim 3, it is characterised in that described detection card is completely set up in cartridge (9), described
The sample pad (1) that well (10) corresponds on the detection card, and the observation window (11) corresponds on the detection card
First detection zone (3), the second detection zone (4) and quality control region (5).
5. effluent piece as claimed in claim 4, it is characterised in that the length of the cartridge is 80-90.0mm, width 10-
20mm, and height is 4-5mm.
6. effluent piece as claimed in claim 1, it is characterised in that the length of the effluent piece is 60-70mm, and width is 3-
4mm。
7. effluent piece as claimed in claim 1, it is characterised in that the quality control region (5) includes the 3rd antibody, and the described 3rd is anti-
Body specifically binds to the first antibody and secondary antibody.
8. effluent piece as claimed in claim 1, it is characterised in that described the first detection zone (3), the second detection zone (4) and
Quality control region (5) is located on nitrocellulose filter (6).
9. a kind of kit for being used to detect clenbuterol hydrochloride, it is characterised in that the kit includes：
(i) container；And
(ii) the effluent piece of the detection lean meat Compounds as described in claim 1-8 is any.
10. kit as claimed in claim 9, it is characterised in that the kit also includes specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720857300.0U CN207020195U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting lean meat Compounds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720857300.0U CN207020195U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting lean meat Compounds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207020195U true CN207020195U (en) | 2018-02-16 |
Family
ID=61484054
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720857300.0U Active CN207020195U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting lean meat Compounds |
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| Country | Link |
|---|---|
| CN (1) | CN207020195U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110275013A (en) * | 2019-05-22 | 2019-09-24 | 克拉玛依市市场监督管理局 | Cimaterol colloidal gold rapid detection card and preparation method thereof suitable for animal derived food |
| CN110275012A (en) * | 2019-05-22 | 2019-09-24 | 克拉玛依市市场监督管理局 | It is applicable in animal derived food bambuterol colloidal gold rapid detection card and preparation method |
-
2017
- 2017-07-14 CN CN201720857300.0U patent/CN207020195U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110275013A (en) * | 2019-05-22 | 2019-09-24 | 克拉玛依市市场监督管理局 | Cimaterol colloidal gold rapid detection card and preparation method thereof suitable for animal derived food |
| CN110275012A (en) * | 2019-05-22 | 2019-09-24 | 克拉玛依市市场监督管理局 | It is applicable in animal derived food bambuterol colloidal gold rapid detection card and preparation method |
| CN110275012B (en) * | 2019-05-22 | 2022-07-08 | 克拉玛依市市场监督管理局 | Bambuterol colloidal gold rapid detection card suitable for animal derived food and preparation method thereof |
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