CN207020191U - A kind of effluent piece for detecting mycotoxin - Google Patents
A kind of effluent piece for detecting mycotoxin Download PDFInfo
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- CN207020191U CN207020191U CN201720856772.4U CN201720856772U CN207020191U CN 207020191 U CN207020191 U CN 207020191U CN 201720856772 U CN201720856772 U CN 201720856772U CN 207020191 U CN207020191 U CN 207020191U
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Abstract
The utility model discloses a kind of effluent piece for detecting mycotoxin, the effluent piece includes a detection and blocked, and described detection card includes backing, and sample pad, gold standard pad, nitrocellulose filter, detection zone, quality control region and absorption pad on backing.Effluent piece of the present utility model can detect aflatoxins, T2 toxin, zearalenone simultaneously, suitable for the quick detection of mycotoxin.
Description
Technical field
Detection inspection technology field is the utility model is related to, by antigen-antibody immune response in immunochemistry by means of layer
Analysis technology reaches testing goal, more particularly to a kind of effluent piece for detecting mycotoxin.
Background technology
Fungi, it is that one kind has cell membrane, without chlorophyll, unrooted base of leaf, is survived, can be had with saprophytic or parasitic method
Property or the eukaryotic microorganisms of vegetative propagation.All kinds of Mushrooms are most commonly that in fungi, in addition also mould and yeast.Although fungi
It is widely used in food industry, such as makes wine, the manufacture of sauce processed, bread, benefit, but some fungies are brought to mankind's production and living
Also can be endangered by producing mycotoxin contaminated food products this mode to human body health care belt.
Mycotoxin is mycetogenetic secondary metabolite, mainly including aflatoxin, fusarium toxin etc..Earliest
The description that mycotoxin causes poisoning is just related in 11 Century European icon paintings, but until more than 100,000 fire of nineteen sixty Britain
After the chicken event dead because of the feed of feeding aflatoxin contamination occurs, mycotoxin is just re-recognized by people.By complete
The influence of the factors such as ball climate warming, humidity, edible and feeding agricultural product are on the rise by mycotoxin pollution, and long-term consumption is true
Verticillium toxin contaminated food products, not only causes three causes of being poisoned but also cause, carcinogenic, cause strange, mutagenesis, all over the world for mycotoxin
The report of pollution increasingly increases.
China realizes that behave is not in place, and mycotoxin pollution is even more serious plus people due to geographical climate environment feature,
Counted according to processing of farm products research institute of the Chinese Academy of Agricultural Sciences, 2001 to 2011 years are during the decade, influenceed by mycotoxin pollution, I
State exports European Union's food relevant contravention up to 2559, and wherein mycotoxin is exceeded accounts for 28.6%, higher than heavy metal known to the public,
The factors such as food additives, agricultural residual, the ratio highest in single incident.Academy of agricultural sciences processes institute researcher Liu Yang and thought, very
The exceeded maximum obstruction for having turned into agricultural products in China outlet European Union of verticillium toxin, is caused huge to the processing of China's grain and oil and export enterprise
Big economic loss.
The common method of mycotoxin detection includes：Thin-layered chromatography (TLC), high performance liquid chromatography (HPLC), ELISA
Method etc..Above-mentioned detection method needs to coordinate detecting instrument to use, and detection time is longer, complex operation, is not easy to live inspection
Survey.Therefore, the reagent and method of detection mycotoxin easy to use, reliable results are badly in need of in this area.
Utility model content
The purpose of this utility model is to provide a kind of effluent piece of detection mycotoxin easy to use, reliable results.
In first aspect of the present utility model, there is provided a kind of effluent piece for detecting mycotoxin, the effluent piece include
One detection card, described detection card include backing (0), and the following structure for proximally arriving distal end on the backing：
Sample pad (1), gold standard pad (2), nitrocellulose filter (8), detection zone, quality control region (6) and absorption pad (7)；
And described gold standard pad (2) includes：First antibody load region, secondary antibody load region and the load of the 3rd antibody
Area, wherein, described first antibody is directed to the antibody of aflatoxins for the specificity of colloid gold label, and described secondary antibody is
The specificity of colloid gold label is directed to the antibody of T2 toxin, and the 3rd described antibody is directed to corn for the specificity of colloid gold label
The antibody of zeranol；
And the detection zone includes：First detection zone (3), the second detection zone (4) and the 3rd detection zone (5), wherein, institute
The first detection zone (3) stated is coated with the comlete antigen of aflatoxins, and described the second detection zone (4) is coated with the complete of T2 toxin
Holoantigen, the 3rd described detection zone (5) are coated with the comlete antigen of zearalenone.
In another preference, the comlete antigen of described aflatoxins is the conjugate of aflatoxins and carrier protein.
In another preference, the comlete antigen of described T2 toxin is the conjugate of T2 toxin and carrier protein.
In another preference, the comlete antigen of described zearalenone is zearalenone and carrier protein
Conjugate.
In another preference, the carrier protein is selected from the group：BSA, OVA or its combination.
In another preference, described backing (0) is PVC board.
In another preference, the sample pad (1) is glass fibre.
In another preference, the absorption pad (7) is blotting paper.
In another preference, described sample pad (1), gold standard pad (2), nitrocellulose filter (8) and absorption pad (7)
It is pasted on the backing.
In another preference, described sample pad (1) and gold standard pad (2) partly overlap.
In another preference, described detection zone is positioned close to the side of gold standard pad (2), and described quality control region
(6) it is positioned close to the side of absorption pad (7).
In another preference, the effluent piece also includes cartridge (9), and the well (10) in the cartridge
With observation window (11).
In another preference, described detection card is completely set up in cartridge (9), and the well (10) corresponds to institute
The sample pad (1) on detection card is stated, and the observation window (11) corresponds to detection zone and the quality control region that the detection blocks.
In another preference, the length of the cartridge is 80-90.0mm, width 10-20mm, and height is 4-5mm.
In another preference, the length of the effluent piece is 60-70mm, and width is 3-4mm.
In another preference, the quality control region (6) includes the 4th antibody, and the 4th antibody specificity is incorporated into described
First antibody, secondary antibody and the 3rd antibody.
In another preference, described the first detection zone (3), the second detection zone (4), the 3rd detection zone (5) and Quality Control
Area (6) is located on nitrocellulose filter (8).
In another preference, the effluent piece is used to detect the mycotoxin in food or feed.
In another preference, the food includes milk, feed, cereal, food oils and pulses.
In second aspect of the present utility model, there is provided a kind of kit for being used to detect mycotoxin, the kit
Including：
(i) container；And
(ii) the effluent piece of the detection mycotoxin as described in the utility model first aspect.
In another preference, the kit also includes specification.
It should be understood that in the scope of the utility model, above-mentioned each technical characteristic of the present utility model and below (such as implementation
Example) in specifically describe each technical characteristic between can be combined with each other, so as to form new or preferable technical scheme.It is limited to
Length, no longer tire out one by one state herein.
Brief description of the drawings
Fig. 1 is a kind of structure sectional view of the detection card of preference of the utility model.Wherein, 1 is sample pad, and 2 be gold mark
Pad, 3 be the first detection zone, and 4 be the second detection zone, and 5 be the 3rd detection zone, and 6 be quality control region, and 7 be absorption pad, and 8 be cellulose nitrate
Plain film, 0 is backing.
Fig. 2 is a kind of top view of the effluent piece of the detection mycotoxin of preference of the utility model.Wherein, 9 be cartridge,
10 be well, and 11 be observation window.
Embodiment
The present inventor by depth studying extensively, it has unexpectedly been found that a kind of effluent for detecting mycotoxin in food
Piece.Specifically, according to detection when develop the color position difference, the effluent piece can simultaneously a variety of mycotoxins of quick detection, including but
Aflatoxins, T2 toxin and zearalenone are not limited to, changes the previous reagent of mesh and only detects a kind of present situation of composition.Herein
On the basis of, complete the utility model.
Mycotoxin
Mycotoxin is metabolite caused by fungi grows in food or feed, all harmful to human and animal.
As used herein, described " aflatoxins ", " aflatoxin " are used interchangeably, and are that a kind of chemical constitution is similar
Compound, be dihydrofuran cumarin derivative.Aflatoxin is mainly by aspergillus flavus (aspergillus
Flavus), secondary metabolite caused by aspergillus parasiticus (a.parasiticus), occurs in damp-heat area food and feed
The probability highest of aflatoxin.They are present in soil, animals and plants, various nuts, particularly easily pollute peanut, jade
The grain oil products such as rice, rice, soybean, wheat, it is mycotoxicosis maximum, to the extremely prominent one kind of human health risk
Mycotoxin.
T2 toxin (T2) is a kind of mycotoxin as caused by a variety of sickle-like bacteria.The grains such as main pollution wheat, barley, corn
Food crop and its product, larger harm is constituted to human health and animal husbandry.T-2 toxin mainly influences blood, liver, kidney
It is dirty, the function of pancreas muscle and lymphocyte, the general clinical symptoms after T-2 toxin poisonings are apocleisis, vomiting, diarrhoea, growth
Stagnation, breeding and dysneuria etc..
Zearalenone (Zearalenone) is also known as F-2 toxin, and it is separated from the corn for have head blight first
Arrive.Its toxigenic bacterium of zearalenone is mainly the bacterium dwarf of Fusarium (Fusarium), such as Fusarium graminearum
And fusarium tricinctum (F.tricinctum), yellow fusarium (Fusarium culmorum), scouring rush's sickle (F.graminearum)
Spore (Fusarium equiseti), F.semitectum (Fusarium sernitectum), fusariun solani (Fusarium
The strain such as solani).Zearalenone mainly pollutes the cereal such as corn, wheat, rice, barley, millet and oat.It is wherein beautiful
The positive rate of rice is 45%, and highest toxic amount can reach 2909mg/kg；The recall rate of wheat is 20%, and toxic amount is
0.364~11.05mg/kg.The heat resistance of zearalenone is stronger, and 1h is handled at 110 DEG C and is just totally disrupted.
As used herein, described " mycotoxin to be measured " refers to the mycotoxin of lateral flow strip detection of the present utility model,
Specifically include aflatoxins, T2 toxin and zearalenone.
Detection method and operation principle
For the ease of understanding the utility model, the Cleaning Principle of the utility model effluent piece is provided.It should be understood that this practicality is new
The protection domain of type is not influenceed or limitation by the principle.
The utility model use competition law immunochromatography principle, by the comlete antigen (the first compound) of aflatoxins,
The comlete antigen (the second compound) of T2 toxin and the comlete antigen (triplex thing) of zearalenone are individually fixed in
The first detection zone, the second detection zone and the 3rd detection zone (solid phase antigen) on nitrocellulose membrane, by the special of colloid gold label
Property for the antibody (first antibody) of aflatoxins, colloid gold label specificity for T2 toxin antibody (secondary antibody) with
And the specificity of colloid gold label is fixed on gold standard pad for the antibody (the 3rd antibody) of zearalenone, and formed by Fig. 1
In detecting strip and being loaded.After sample to be checked is added in the sample pad of detection card one end by well, pass through capillarity
Move forward, dissolving combines first antibody, secondary antibody and the 3rd antibody of the colloid gold label in gold standard pad, then moves successively
To the first detection zone, the second detection zone, the 3rd detection zone, quality control region.If mycotoxin to be measured, collaurum are not contained in sample
First antibody, secondary antibody and the 3rd antibody of mark can be fixed on the first compound on nitrocellulose filter, respectively
Two compounds and the capture of triplex thing, so as to form red stripes in the first detection zone, the second detection zone, the 3rd detection zone.
If containing finite concentration certain mycotoxin to be measured in sample, such as contain aflatoxins, the of colloid gold label in gold standard pad
One antibody can be combined first with aflatoxins in sample after being dissolved, so as to suppress first antibody and the on nitrocellulose filter
The combination of one compound, the band color of the first detection zone will weaken or disappear；Secondary antibody and the knot of the second compound
The combination of conjunction, the 3rd antibody and triplex thing is unaffected, and the second detection zone and the 3rd detection zone still form red bar
Band.As contained two or more mycotoxins to be measured in sample simultaneously, such as contain aflatoxins, T2 toxin and Gibberella zeae simultaneously
Ketenes, first antibody, secondary antibody and the 3rd antibody and the first compound on nitrocellulose filter, second of colloid gold label
The combination of compound and triplex thing can be suppressed, the first detection zone, the second detection zone, the band face of the 3rd detection zone
Color can weaken or disappear.In other words, when containing certain mycotoxin in sample to be checked, corresponding antibody and detection
The combination in region is affected, and the band color of corresponding detection zone can weaken or disappear.
The utility model is provided with the 4th antibody in the quality control region of nitrocellulose filter, and the 4th antibody specificity combines
In the first antibody, secondary antibody and the 3rd antibody, no matter whether contain mycotoxin to be measured, collaurum mark in measuring samples
First antibody, secondary antibody and the 3rd antibody of note can form red stripes, the band with the 4th antibody binding of quality control region
It is to judge whether chromatography process normal and whether detection plate fails standard, while also serves as control difference between batch in quantitative analysis
Parameter.
Sensitivity
Effluent piece sensitivity of the present utility model is as follows,
Aflatoxin B1 detects thresholding：2.5ug/l
T2 Mycotoxin identification thresholdings：100ug/l
Zearalenone detects thresholding：60ug/l
The manufacture of effluent piece
In the utility model, the existing material system in this area can be selected in each element (or component) of the effluent piece
Into.
Backing 0 can be made of any stabilization, non-porous material, and its intensity should be enough supporting material and adhere thereto
Each element.Because many measure are by the use of water as dispersive medium, therefore backing 0 is preferably substantially water-impervious.One
In individual preference, backing 0 is made of polymer film, is used made of polychloroethylene film (such as PVC offset plates).
Sample pad 1 can be made of any absorbent material.Workable example of material includes：Cellulose, nitrocellulose,
Cellulose acetate, glass fibre, nylon, polyelectrolyte ion exchange membrane, propylene copolymer/nylon and polyether sulfone.
Absorption pad 7 can be made of any material that can be absorbed as sample and the liquid of buffer solution.The absorption of absorption pad 7
Ability is sufficiently large, to absorb the liquid for being added to test-strips.Suitable for absorption pad 7 material example include cellulose and
Absorbent filter.
Antibody
First antibody, secondary antibody and the 3rd antibody are the antibody of colloid gold label, can respectively with aflatoxins, T2
Toxin, zearalenone combine.
4th antibody is sheep anti-mouse igg in combination with first antibody, secondary antibody and the 3rd antibody, preferable 4th antibody
It is more anti-.
Antibody used in the utility model is commercially available.
Colloid gold label
Collaurum is to be acted on by gold chloride (HAuCl4) in reducing agent such as white phosphorus, ascorbic acid, sodium citrate, tannic acid etc.
Under, a certain size gold grain is can be grouped to, and because electrostatic interaction turns into a kind of colloidal state of stabilization, formed electronegative
Hydrophobic sol solution, turn into stable colloidal state due to electrostatic interaction, therefore claim collaurum.
Collaurum is negatively charged under mild alkaline conditions, can form firm combination with the positive charge group of protein molecule,
Because this combination is electrostatical binding, so not influenceing the biological nature of protein.Collaurum in addition to being combined with protein,
It can also be combined with many other large biological molecules, such as SPA, PHA, ConA.It is such as high according to some physical behaviors of collaurum
Electron density, granular size, shape and color reaction, plus the immune and biological characteristics of conjugate, thus make collaurum wide
It is applied to the fields such as immunology, histology, pathology and cell biology generally.
The macromolecules such as colloid gold label, substantially protein are adsorbed to the coating process on colloid gold particle surface.Inhale
Random reason is probably colloid gold particle surface negative charge, and firm knot is formed because of Electrostatic Absorption with the positive charge group of protein
Close.The colloid gold particle of various different-grain diameters, namely different colours can be easily prepared from gold chloride with reducing process.It is this
Spherical particle has very strong adsorption function to protein, can be with staphylococcal protein A, immunoglobulin, toxin, sugared egg
In vain, the Non-covalent binding such as enzyme, antibiotic, hormone, bovine serum albumin(BSA), polypeptide, thus in basic research and clinical trial
As highly useful instrument.
Immuno-gold labeling technology (Immunogold labelling technique) mainly make use of gold grain to have height
The characteristic of electron density, marked in gold at protein binding, under the microscope visible dark brown coloured particles, when these labels are corresponding
When largely assembling at part, naked eyes red color visible or pink spot, thus detected for qualitative or sxemiquantitative tachysynthesis
In method, this reaction can also be exaggerated by the deposition of Argent grain, referred to as immunogold silver staining.
In the preparation of the utility model lateral flow strip, it is preferable that 40nm collaurums are taken, with 0.1M K2CO3PH is adjusted, with every milli
Rising the μ g antibody of collaurum 5 to be marked, must be precipitated after marking fluid centrifugation, precipitation 10mM pH7.2PBS dissolve, and in 650nm
Lower measure light absorption value OD, apply on paving polyester material and dry with 1-3OD, as the gold standard pad in the utility model detection card.
The preparation of lateral flow strip
Each element (or component) of the utility model effluent piece is using technical method manufacture generally in the art and group
Dress.
Preferably, the first compound, the second compound, triplex thing and Quality Control antibody are diluted with coating buffer solution, will
Four kinds of capture liquid after dilution are equably coated on the nitrocellulose membrane as detection part respectively, and four kinds of capture liquid penetrate into
The first detection zone, the second detection zone, the 3rd detection zone and quality control region are formed after nitrocellulose filter respectively.
Sample pad, gold standard pad, nitrocellulose filter, absorption pad are pasted on backing by adhesive respectively.With slitting
Machine-cut is placed in cartridge, well alignment detection card sample pad location into 4mm detection card, detection reagent is assembled into after compression
Box, add drier foil sealing packaging and preserve.
The advantages of the utility model：
The effluent piece of detection mycotoxin described in the utility model overcome need to detect one by one in existing method it is not of the same race
The drawbacks of class mycotoxin, by same lateral flow strip set three detection zones, quickly, efficiently and accurately specific detection it is more
Kind mycotoxin.
Below in conjunction with specific embodiment, the utility model is further illustrated.It should be understood that following description is only this practicality
New most preferred embodiment, and it is not construed as the limitation for scope of protection of the utility model.Fully understanding
On the basis of the utility model, generally according to normal condition, or according to the condition proposed by manufacturer, those skilled in the art
Nonessential change can be made to the technical solution of the utility model, such change should be considered as being included in this practicality newly
Among the protection domain of type.
Embodiment 1
The structure of effluent piece
The effluent piece (or test-strips) of detection mycotoxin of the present utility model includes cartridge and the detection in cartridge
Card.
A kind of structure of preferable detection card is as shown in figure 1, it includes backing 0, and on backing：Sample pad 1,
Gold standard pad 2, nitrocellulose filter 8, detection zone (or detection line), including the first detection zone 3 and the detection of the second detection zone the 4, the 3rd
Area 5, quality control region 6 (or check plot, control line, nature controlling line) and absorption pad 7.
A kind of structure of preferable cartridge 9 as shown in Fig. 2 include well 10 and observation window 11 thereon.It is also, described
Detection card is completely set up in cartridge 9, the sample pad 1 that the well 10 corresponds on the detection card, and the observation window
11 the first detection zone 3, the second detection zone 4, the 3rd detection zone 5 and the quality control regions 6 corresponded on the detection card
Specifically, effluent piece of the present utility model has the characteristics that：
The length of backing 0 is identical with effluent piece.
Sample pad 1 is located at one end of test-strips, and absorption pad 7 is located at the other end of test-strips.
Three kinds of antibody are loaded with gold standard pad 2, first antibody is directed to the anti-of aflatoxins for the specificity of colloid gold label
Body, secondary antibody are directed to the antibody of T2 toxin for the specificity of colloid gold label, and the 3rd antibody is the specificity of colloid gold label
For the antibody of zearalenone, above-mentioned antibody is commercially available.
Detection zone (also referred to as detection line) is between sample pad 1 and absorption pad 7, including the first detection zone 3, the second detection zone
4 and the 3rd detection zone 5, it is preferable that the first detection zone 3 is located at close to one end of sample pad 1, and usual detection zone is arranged on nitric acid fibre
Tie up on plain film 8, pass through the gold standard pad 2 and adsorptive pads 7 of the nitrocellulose filter linkage flag.Preferably, the cellulose nitrate
Quality control region 6 (also referred to as nature controlling line) is additionally provided with plain film 8, quality control region 6 is between detection zone and absorption pad 7.
Wherein, the first described detection zone 3 is coated with the comlete antigen of aflatoxins；The second described detection zone 4 is coated with
There is the comlete antigen of T2 toxin, the 3rd described detection zone 5 is coated with the comlete antigen of zearalenone.Quality control region 6 is coated with
There is the 4th antibody, the 4th antibody specificity is incorporated into described first antibody, secondary antibody and the 3rd antibody.
Embodiment 2
The use of effluent piece
1. taking the representational grain samples of more than 5g to crush and (cross 20 mesh sieves), the present embodiment accurately claims using wheat as sample
Take 2g uniformly to crush sample to be added in centrifuge tube；2ml dilutions 1 and ethyl acetate 8mL are accurately added into centrifuge tube, by bottle
Gag tightening seal, with forced oscillation 5 minutes, 4000rpm was centrifuged 1 minute；2mL supernatants are taken into small glass with suction pipe, are dried up
Filtrate, then measure 0.4mL dilutions 2 and redissolve bottom of a cup solid.This lysate is to detect liquid.
2. detection card is kept flat, sample solution to be checked is drawn with dropper, vertical dropwise addition 3 is dripped in well, opened after sample-adding
Beginning timing.
3. read testing result in 5 minutes.
As a result judge：
A：First detection zone, the second detection zone, the 3rd detection zone and quality control region change colour (red) simultaneously, show in sample not
It is less than minimum detected value containing mycotoxin to be measured or its concentration, testing result is feminine gender.
B:Quality control region changes colour (red), and the first detection zone, the second detection zone and the 3rd detection zone are non-discolouring, show sample
In contain aflatoxins, T2 toxin and zearalenone simultaneously, testing result is the positive.
C：Quality control region and some or two detection zone discolorations (red), and other detection zones are non-discolouring, show that sample contains
There is the mycotoxin corresponding with non-discolouring detection zone, testing result is the positive.
D：Quality control region is non-discolouring, and no matter whether detection zone changes colour, and shows that this detection is invalid.
All it is incorporated as referring in this application in all documents that the utility model refers to, just as each document quilt
It is individually recited as with reference to such.In addition, it is to be understood that after above-mentioned instruction content of the present utility model has been read, this area skill
Art personnel can make various changes or modifications to the utility model, and these equivalent form of values equally fall within the application appended claims
Book limited range.
Claims (10)
1. a kind of effluent piece for detecting mycotoxin, it is characterised in that the effluent piece includes a detection and blocked, described detection card
Including backing (0), and the following structure for proximally arriving distal end on the backing：Sample pad (1), gold standard pad (2), nitre
Acid cellulose film (8), detection zone, quality control region (6) and absorption pad (7)；
And described gold standard pad (2) includes：First antibody load region, secondary antibody load region and the 3rd antibody load region, its
In, described first antibody is directed to the antibody of aflatoxins for the specificity of colloid gold label, and described secondary antibody is colloid
The specificity of gold mark is directed to the antibody of T2 toxin, and the 3rd described antibody is directed to Gibberella zeae for the specificity of colloid gold label
The antibody of ketenes；
And the detection zone includes：First detection zone (3), the second detection zone (4) and the 3rd detection zone (5), wherein, it is described
First detection zone (3) is coated with the comlete antigen of aflatoxins, and described the second detection zone (4) is coated with the completely anti-of T2 toxin
Original, the 3rd described detection zone (5) are coated with the comlete antigen of zearalenone.
2. effluent piece as claimed in claim 1, it is characterised in that described detection zone is positioned close to the one of gold standard pad (2)
Side, and described quality control region (6) is positioned close to the side of absorption pad (7).
3. effluent piece as claimed in claim 1, it is characterised in that the effluent piece also includes cartridge (9), and positioned at described
Well (10) and observation window (11) in cartridge.
4. effluent piece as claimed in claim 3, it is characterised in that described detection card is completely set up in cartridge (9), described
The sample pad (1) that well (10) corresponds on the detection card, and the observation window (11) corresponds on the detection card
Detection zone and quality control region (6).
5. effluent piece as claimed in claim 4, it is characterised in that the length of the cartridge is 80-90.0mm, width 10-
20mm, and height is 4-5mm.
6. effluent piece as claimed in claim 1, it is characterised in that the length of the effluent piece is 60-70mm, and width is 3-
4mm。
7. effluent piece as claimed in claim 1, it is characterised in that the quality control region (6) includes the 4th antibody, and the described 4th is anti-
Body specifically binds to the first antibody, secondary antibody and the 3rd antibody.
8. effluent piece as claimed in claim 1, it is characterised in that described the first detection zone (3), the second detection zone (4),
Three detection zones (5) and quality control region (6) are located on nitrocellulose filter (8).
9. a kind of kit for being used to detect mycotoxin, it is characterised in that the kit includes：
(i) container；And
(ii) the effluent piece of the detection mycotoxin as described in claim 1-8 is any.
10. kit as claimed in claim 9, it is characterised in that the kit also includes specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720856772.4U CN207020191U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting mycotoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720856772.4U CN207020191U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting mycotoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207020191U true CN207020191U (en) | 2018-02-16 |
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ID=61483922
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| CN201720856772.4U Active CN207020191U (en) | 2017-07-14 | 2017-07-14 | A kind of effluent piece for detecting mycotoxin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108097340A (en) * | 2018-02-26 | 2018-06-01 | 北京华科泰生物技术有限公司 | It is a kind of for joint-detection micro-fluidic chip of stomach function disorder in screening and its preparation method and application |
-
2017
- 2017-07-14 CN CN201720856772.4U patent/CN207020191U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108097340A (en) * | 2018-02-26 | 2018-06-01 | 北京华科泰生物技术有限公司 | It is a kind of for joint-detection micro-fluidic chip of stomach function disorder in screening and its preparation method and application |
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