CN206970629U - A kind of paraffin cellular-type ring mediated responses pipe device - Google Patents
A kind of paraffin cellular-type ring mediated responses pipe device Download PDFInfo
- Publication number
- CN206970629U CN206970629U CN201720885439.6U CN201720885439U CN206970629U CN 206970629 U CN206970629 U CN 206970629U CN 201720885439 U CN201720885439 U CN 201720885439U CN 206970629 U CN206970629 U CN 206970629U
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- lid
- centrifuge tube
- paraffin
- pipe
- cellular
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Abstract
The utility model discloses a kind of paraffin cellular-type ring mediated responses pipe device, including centrifuge tube and lid, the centrifuge tube is connected with the lid by linking arm, the top of the centrifuge tube has pipe edge, the centrifuge tube is divided into three sections, centrifuge tube is respectively direct tube section from top to bottom, stage casing, bottom section, the pipe is along positioned at the top of the direct tube section, there is accommodating chamber in the lid, one end of the lid has the projection with the lid integrative-structure, lyophilized polymerase is placed with the accommodating chamber, the lyophilized polymerase is sealed by solid paraffin layer, the solid paraffin layer is bonded in the accommodating chamber bottom, the pipe is along upper with the concave station to cave in, there is step in the lid, the step is adapted to the concave station, the linking arm is made up of bendable material.The paraffin cellular-type ring mediated responses pipe device structure is uncomplicated, and low manufacture cost, use is simple to operate, reduces the complicated degree of loop-mediated isothermal amplification experiment, is provided convenience to test operation, improve test efficiency.
Description
Technical field
Reaction tube field is the utility model is related to, specially a kind of paraffin cellular-type ring mediated responses pipe device.
Background technology
Current loop-mediated isothermal amplification experiment is to add reagent component and DNA to be checked in the small centrifuge tube of thin-walled, reaction
Pipe will pass through 2 stages of reaction, first be denatured 95 ~ 98 DEG C of reaction solution about 1 minute, then be down to 50 ~ 60 DEG C of annealing about 1 minute to carry
High detection susceptibility, then open lid and add lyophilized polymerase, finally in 63 DEG C or so water-baths until reaction terminates.The technology
Due to lyophilized polymerase non-refractory, it is necessary to can be just added to after cooling in reaction system, so reaction is wanted to uncap and add for 2 times
Reagent, experimental implementation is caused to repeat cumbersome, efficiency is low.
Utility model content
The purpose of this utility model is to provide a kind of paraffin cellular-type ring mediated responses pipe device, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the utility model provides following technical scheme：A kind of paraffin cellular-type ring mediated responses pipe
Device, including centrifuge tube and lid, the centrifuge tube are connected with the lid by linking arm, and the top of the centrifuge tube has
Pipe edge, the centrifuge tube are divided into three sections, and centrifuge tube is respectively direct tube section, stage casing, bottom section from top to bottom, and the pipe is along positioned at described
The top of direct tube section, there is accommodating chamber in the lid, one end of the lid has the projection with the lid integrative-structure,
Lyophilized polymerase is placed with the accommodating chamber, the lyophilized polymerase is sealed by solid paraffin layer, the solid paraffin layer
It is bonded in the accommodating chamber bottom.
Preferably, the pipe along upper with the concave station to cave in.
Preferably, there is step, the step is adapted to the concave station in the lid.
Preferably, the linking arm is made up of bendable material.
Compared with prior art, the beneficial effects of the utility model are：The paraffin cellular-type ring mediated responses pipe device makes
Used time, the lyophilized polymerase of non-refractory is pre-applied in the accommodating chamber in lid, then a small amount of melt is added dropwise into accommodating chamber
Paraffin（50-60 DEG C of melting point）, formation solid paraffin layer, which gathers lyophilized polymerase, after paraffin cooling is sealed in lid, then covers
Upper tube cap, the step in lid coordinate with pipe along upper concave station, such design causes lid and centrifuge tube coordinate it is relatively reliable,
The reaction solution of other components and DNA sample to be checked are added during use in centrifuge tube, finally adds a small amount of liquid in sample ullage
Body paraffin, sample liquids are sealed in centrifuge tube after paraffin solidification, when carrying out high-temperature denatured, simply heat centrifuge tube bottom of the tube
Point, the sample in centrifuge tube is by thermal response, and the heating of lid position and unobvious, temperature are not enough to melt the solid in accommodating chamber
Paraffin layer, the lyophilized polymerase activity in lid is protected, and when carrying out 63 degree of water-baths, this step needs lyophilized polymerase
Participate in reaction, now centrifuge tube is turned around 3 minutes, lid contact 63 degree water-baths, make solid paraffin layer liquefy, then reaction solution and
Lyophilized lyophilized polymerase is fully dissolved, and centrifuge tube is just being put to 63 DEG C of water-baths of carry out again afterwards, until experiment terminates, projection
The person of being easy to use pulls out lid；Present apparatus structure is uncomplicated, and low manufacture cost, use is simple to operate, reduces ring mediation
The complicated degree of constant-temperature amplification experiment, provides convenience to test operation, improves test efficiency.
Brief description of the drawings
Fig. 1 is the utility model structure diagram.
Fig. 2 is the structural representation after the utility model lid fastens.
Fig. 3 is the top view of the utility model lid.
In figure：1st, centrifuge tube, 2, lid, 3, linking arm, 4, pipe edge, 5, direct tube section, 6, stage casing, 7, bottom section, 8, accommodating chamber,
9th, pull head, 10, concave station, 11, step, 12, solid paraffin layer, 13, lyophilized polymerase.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-2 is referred to, the utility model provides a kind of technical scheme：A kind of paraffin cellular-type ring mediated responses pipe dress
Put, including centrifuge tube 1 and lid 2, the centrifuge tube 1 and the lid 2 are connected by linking arm 3, the top of the centrifuge tube 1
With pipe along 4, the centrifuge tube 1 divides for three sections, and centrifuge tube 1 is respectively direct tube section 5, stage casing 6, bottom section 7 from top to bottom, the pipe
The top for being located at the direct tube section 5 along 4, has accommodating chamber 8 in the lid 2, in the lid 2 also with and the lid 2
One end there is projection 9 with the integrative-structure of lid 2, lyophilized polymerase 13 is placed with the accommodating chamber 8, it is described lyophilized
Polymerase 13 is sealed by solid paraffin layer 12, and the solid paraffin layer 12 is bonded in the bottom of accommodating chamber 8, and the pipe is along 4
It is upper that there is the concave station 10 to cave in, there is step 11 in the lid 2, the step 11 is adapted to the concave station 10, the connection
Arm 3 is made up of bendable material.
Operation principle：When using the paraffin cellular-type ring mediated responses pipe device, by the lyophilized polymerase of non-refractory
13 are pre-applied in the accommodating chamber 8 in lid 2, then the paraffin melted on a small quantity is added dropwise into accommodating chamber 8（50-60 DEG C of melting point）, stone
Solid paraffin layer 12 is formed after wax cooling lyophilized polymerase poly- 13 is sealed in lid 2, then cover lid 2, in lid 2
Step 11 coordinates with pipe along the concave station 10 on 4, and it is relatively reliable that such design make it that lid 2 and centrifuge tube 1 coordinate, during use from
The reaction solution of other components and DNA sample to be checked are added in heart pipe 1, finally adds a small amount of atoleine in sample ullage,
Sample liquids are sealed in centrifuge tube 1 after paraffin solidification, when carrying out high-temperature denatured, simply heat the ttom of pipe part of centrifuge tube 1, from
Sample in heart pipe 1 is by thermal response, and the heating of the position of lid 2 and unobvious, temperature are not enough to melt the solid stone in accommodating chamber 8
Wax layer 12, the activity of lyophilized polymerase 13 in lid 2 is protected, and when carrying out 63 degree of water-baths, this step needs lyophilized poly-
Synthase 13 participates in reaction, now turns centrifuge tube 13 minutes around, and lid 2 contacts 63 degree of water-baths, solid paraffin layer 12 is liquefied,
Then reaction solution fully dissolves with lyophilized lyophilized polymerase 13, and centrifuge tube 1 is just being put into 63 DEG C of water-baths of carry out again afterwards, until
Experiment terminates, and the person of being easy to use of projection 9 pulls out lid 2.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (4)
1. a kind of paraffin cellular-type ring mediated responses pipe device, including centrifuge tube（1）And lid（2）, it is characterised in that：It is described from
Heart pipe（1）With the lid（2）Pass through linking arm（3）Connection, the centrifuge tube（1）Top there is pipe edge（4）, the centrifugation
Pipe（1）It is divided into three sections, centrifuge tube（1）It is respectively direct tube section from top to bottom（5）, stage casing（6）, bottom section（7）, the pipe edge（4）Position
In the direct tube section（5）Top, the lid（2）It is upper that there is accommodating chamber（8）, the lid（2）One end have with it is described
Lid（2）The projection of integrative-structure（9）, the accommodating chamber（8）Inside it is placed with lyophilized polymerase（13）, the lyophilized polymerase
（13）Pass through solid paraffin layer（12）Sealing, the solid paraffin layer（12）It is bonded in the accommodating chamber（8）Bottom.
A kind of 2. paraffin cellular-type ring mediated responses pipe device according to claim 1, it is characterised in that：The pipe edge
（4）It is upper that there is the concave station to cave in（10）.
A kind of 3. paraffin cellular-type ring mediated responses pipe device according to claim 2, it is characterised in that：The lid
（2）It is upper that there is step（11）, the step（11）With the concave station（10）Adaptation.
A kind of 4. paraffin cellular-type ring mediated responses pipe device according to claim 1, it is characterised in that：The linking arm
（3）It is made up of bendable material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720885439.6U CN206970629U (en) | 2017-07-20 | 2017-07-20 | A kind of paraffin cellular-type ring mediated responses pipe device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720885439.6U CN206970629U (en) | 2017-07-20 | 2017-07-20 | A kind of paraffin cellular-type ring mediated responses pipe device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206970629U true CN206970629U (en) | 2018-02-06 |
Family
ID=61403385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720885439.6U Expired - Fee Related CN206970629U (en) | 2017-07-20 | 2017-07-20 | A kind of paraffin cellular-type ring mediated responses pipe device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206970629U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641899A (en) * | 2018-05-24 | 2018-10-12 | 金陵科技学院 | A kind of pre- point of lid of centrifuge tube |
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
| CN111939996A (en) * | 2020-10-19 | 2020-11-17 | 博奥生物集团有限公司 | In-test tube reagent storage method and reagent storage reaction detection integrated test tube |
| CN112516092A (en) * | 2020-12-14 | 2021-03-19 | 上海简逸生物科技有限公司 | Freeze-dried preparation manufacturing process with co-existing redissolution and freeze-dried spheres and freeze-dried preparation |
| WO2021180239A1 (en) * | 2020-03-10 | 2021-09-16 | 苏州先达基因科技有限公司 | Anti-pollution single tube nucleic acid isothermal amplification detection system |
-
2017
- 2017-07-20 CN CN201720885439.6U patent/CN206970629U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641899A (en) * | 2018-05-24 | 2018-10-12 | 金陵科技学院 | A kind of pre- point of lid of centrifuge tube |
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
| WO2021180239A1 (en) * | 2020-03-10 | 2021-09-16 | 苏州先达基因科技有限公司 | Anti-pollution single tube nucleic acid isothermal amplification detection system |
| CN111939996A (en) * | 2020-10-19 | 2020-11-17 | 博奥生物集团有限公司 | In-test tube reagent storage method and reagent storage reaction detection integrated test tube |
| CN111939996B (en) * | 2020-10-19 | 2021-01-29 | 博奥生物集团有限公司 | In-test tube reagent storage method and reagent storage reaction detection integrated test tube |
| CN112516092A (en) * | 2020-12-14 | 2021-03-19 | 上海简逸生物科技有限公司 | Freeze-dried preparation manufacturing process with co-existing redissolution and freeze-dried spheres and freeze-dried preparation |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180206 Termination date: 20180720 |
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| CF01 | Termination of patent right due to non-payment of annual fee |