CN206858591U - A kind of Embryo Culture unit - Google Patents
A kind of Embryo Culture unit Download PDFInfo
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- CN206858591U CN206858591U CN201720608483.2U CN201720608483U CN206858591U CN 206858591 U CN206858591 U CN 206858591U CN 201720608483 U CN201720608483 U CN 201720608483U CN 206858591 U CN206858591 U CN 206858591U
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- embryo
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- embryo culture
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- culture
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 145
- 238000005070 sampling Methods 0.000 claims abstract description 22
- 239000012780 transparent material Substances 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000741 silica gel Substances 0.000 claims description 20
- 229910002027 silica gel Inorganic materials 0.000 claims description 20
- 230000007704 transition Effects 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 5
- 231100000252 nontoxic Toxicity 0.000 claims description 5
- 230000003000 nontoxic effect Effects 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 3
- 230000008602 contraction Effects 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000009738 saturating Methods 0.000 claims description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 20
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 11
- 239000001569 carbon dioxide Substances 0.000 abstract description 10
- 239000001963 growth medium Substances 0.000 abstract description 9
- 238000011534 incubation Methods 0.000 abstract description 7
- 238000000338 in vitro Methods 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 210000002459 blastocyst Anatomy 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 73
- 238000005452 bending Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 206010038743 Restlessness Diseases 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 210000002308 embryonic cell Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to Embryo Culture technical field, it is related to a kind of Embryo Culture unit, culture unit main body including transparent material, the upper surface of culture unit main body opens up a sample introduction end plug hole and a sampling end consent, plug seal is filled in sample introduction end plug hole and sampling end consent, the bottom in sample introduction end plug hole sets sample holes, the bottom of sampling end consent sets thief hole, the underface of thief hole sets embryo's accommodating chamber, cultivate and Embryo Culture chamber is set in unit main body, Embryo Culture chamber connects sample holes by sample intake passage, Embryo Culture chamber connects embryo's accommodating chamber by sample output passage.This Embryo Culture unit loads with after the good formula culture medium of carbon dioxide pre-balance, constitute the In vitro culture system of an absolute tightness, embryonated egg ectogenesis can be supported to blastocyst stage directly in 37 DEG C of common insulating boxs, without CO2gas incubator, it is easy to observe the development of embryo, incubation step is significantly simplified, improves operating efficiency and quality.
Description
Technical field
The utility model belongs to Embryo Culture fixture technology field, is related to a kind of Embryo Culture unit.
Background technology
Current inseminatio externalis and Embryo Culture are completed in CO2gas incubator, rely on temperature constant in incubator
The carbon dioxide of degree and constant density is interacted and exchanged with the chemical composition in culture medium, to maintain smart ovum insemination and embryo
Physiological environment needed for growth.But still exist either on hardware device (culture apparatus or incubator) in existing culture systems
Some defects or deficiency all be present in terms of culture medium.For hardware, existing culture apparatus (incubator) is one open
System, the gas in CO2gas incubator needs constantly exchange with the air outside case i.e. in embryo experiments room and could maintain to train
Support in case or in device gas balance and stability, therefore there is following clearly disadvantageous or defect in such open system：
First, the gas concentration and temperature in device are actually to be in a kind of dynamic poised state, gas concentration and temperature are certain
In the range of fluctuate, the size of fluctuation then depends on the enabling frequency of the model of device or incubator, quality and incubator；Second,
Volatile organic matter in laboratory, harmful physical and chemical factor such as ozone etc. can pass through this open gas communication system
Enter in CO2gas incubator, cause the damage of embryo, in addition it is dead；The third is when CO2gas incubator opens the door,
Gas in case largely overflows and fluctuated with inevitably resulting in the gas concentration in incubator and high temperature, with enabling
The increase of number, the degree that this big ups and downs cause embryo to injure also increase therewith.
Utility model content
The purpose of this utility model is to provide a kind of Embryo Culture unit, advance when Embryo Culture unit is made
Loading can be cultivated to blastaea or required embryonic development with the good culture medium of carbon dioxide pre-equilibration, addition embryonated egg or embryo
Stage, during use only need in 37 DEG C of insulating boxs rewarming, without being balanced in CO2gas incubator, when observing embryo
Embryo need not be taken out, whole device need to only be taken out, be placed in the micro- Microscopic observation with temperature-constant plate or take pictures.During observation
The microenvironment of Embryo Culture will not be caused to produce any change or fluctuation, make the microenvironment of embryo growth in whole incubation
Keep constant.
To achieve these goals, the utility model employs following technical proposals.A kind of Embryo Culture unit, including it is saturating
The culture unit main body of bright material, the upper surface for cultivating unit main body open up a sample introduction end plug hole and a sampling end consent,
Plug seal is filled in sample introduction end plug hole and sampling end consent, the bottom in sample introduction end plug hole sets sample holes, sampling end consent
Bottom sets thief hole, and the underface of thief hole sets embryo's accommodating chamber, Embryo Culture chamber, embryo are set in culture unit main body
Culture chamber connects sample holes by sample intake passage, and Embryo Culture chamber connects embryo's accommodating chamber by sample output passage.
A kind of preferable technical scheme, the most narrow place of sample intake passage are located at upper end, and sample holes gradually taper up, seamlessly transit to
The most narrow place of sample intake passage, then sample intake passage gradually amplify, sample intake passage lower end connects with Embryo Culture chamber；Sample output passage
Lower end connects Embryo Culture chamber, and sample output passage gradually tapers up, gradually amplification again after the most narrow place of sample output passage, using smooth circle
Arc transition connects with embryo's accommodating chamber.It is indirect in short-term when being designed to significantly reduce at both ends open by slot due to opening plug
Tactile air may cause the minor fluctuations of Embryo Culture intracavitary nutrient solution chemical factors, so as to farthest ensure culture ring
The constancy in border.
The junction of a kind of preferable technical scheme, Embryo Culture chamber and sample intake passage is by rapid desufflation section transition, soon
Fast contraction section, which plays, prevents embryonated egg or embryo to be back to sample intake passage.
A kind of preferable technical scheme, sample intake passage are connected with the junction of Embryo Culture chamber by one section of transition arc,
The bending direction and Embryo Culture cavity wall bending direction of the bending direction of transition arc and the sample intake passage wall of the junction on the contrary,
Certain transition arc should be tangent with sample intake passage and Embryo Culture chamber, to ensure that embryonated egg smoothly flows into Embryo Culture chamber.
A kind of preferable technical scheme, the narrowest diameter of sample intake passage are not more than 1.5mm, and the most narrow place of sample output passage leads to
Road diameter is 1.5-2.0mm.
A kind of preferable technical scheme, from sample holes to sample intake passage, then to Embryo Culture chamber, then to sample output passage, then
Whole vias inner walls to embryo's accommodating chamber seamlessly transit and smooth, and without turning and burr, corner uses
Tangent arc transition so that embryonated egg or embryonic cell slidably, are not tangled.
A kind of preferable technical scheme, culture unit main body use glass material, can also use other arbitrarily to embryo
Nontoxic transparent material, plug use silica gel plug, and silica gel plug elasticity is good, is easy to seal, and nontoxic to embryo.
A kind of preferable technical scheme, the bottom of Embryo Culture chamber is curved, ensures in incubation, embryonated egg or embryo
Pool together, and rest on the lowest part of Embryo Culture chamber.
A kind of preferable technical scheme, the bottom of embryo's accommodating chamber is curved, and the minimum point of embryo's accommodating chamber is located at
The underface of thief hole, conveniently takes embryo.
Multiple tracks transverse direction fin, can be adopted in the side wall of a kind of preferable technical scheme, sample introduction end plug hole and sampling end consent
With shape of threads, or thread like fin, horizontal fin can extrude silica gel plug, play sealing effectiveness, ensure air tight.
A kind of preferable technical scheme, the aperture outer of sample holes are higher than the base plane in sample introduction end plug hole so that aperture
Outer can squeeze into silica gel plug bottom surface, ensure sealing effectiveness.Similarly, the aperture outer of thief hole is higher than the bottom of sampling end consent
Plane.
A kind of a diameter of 10mm of preferable technical scheme, sample introduction end plug hole and sampling end consent.Sample holes are diameter 4mm
It is round, thief hole is ellipse, and optimal thief hole is major axis 6mm, short axle 3mm ellipse.
A kind of preferable technical scheme, the surface for cultivating unit main body set breach, and breach is used to fix elastic band, elastic
The fixed silica gel plug of band, prevents silica gel plug from loosening, or have other structures for preventing plug from surprisingly loosening.
A kind of preferable technical scheme, culture unit main body upper surface sets the neck for placing silica gel plug, in order to extract
Fixed placement after silica gel plug, unrest is not mixed up or is dropped.
The advantages of the utility model：1. being previously implanted in advance using the good embryo culture medium of carbon dioxide balance, jump a queue close
It is honored as a queen and independently cultivates unit as the embryo of instant, user is in use, cultivate unit after 37 DEG C of rewarmings directly from sample holes
The embryo of fertilised egg or different developmental phases is added, and is positioned in common insulating box and cultivates to the required stage of development i.e.
Can.Operate extremely convenient, simple, not only significantly reduce working strength, improve operating efficiency, and considerably reduce behaviour
Make the risk slipped up；2. user need not use carbon dioxide balance culture medium again before using, significantly reduce Embryo Culture room
The usage amount of usage amount and carbon dioxide to CO2gas incubator, the expense that saves improve culturing room's safety again
Property；3. due to Embryo Culture unit volume very little, preferable volume is 55mm*25mm*35mm (the long wide * of * are high), and a standard is big
Small insulating box can at least place 200 Embryo Culture units, cultivated simultaneously by every CO2gas incubator and be no more than two
If the recommendation usage of personal embryo, if using this Embryo Culture unit, the culture capacity of a standard size insulating box
Capacity is used with the CO2gas incubator under conventional culture conditions equivalent to 100, therefore has not only saved substantial amounts of reality
Room space, carbon dioxide are tested, also improves the security of embryo experiments room；4. Embryo Culture unit is one independent close
Close culture systems, the temperature and pH value in culture chamber can keep the constancy of height, will not be opened the door with constant incubator and
Change, will not interfere with each other between different cultures, any variation of the external environment in addition to culturing room powers off for a long time or
Accident (such as ultraviolet light of laboratory sterilization) does not interfere with cultivation result, Embryo Culture quality is not interfered with, so as to big
Amplitude improves the training quality of embryo, and the safety and stability of in vitro culture.Even if powering off in addition, will can also train
Support unit to take out, continue to cultivate by body temperature, this characteristic is also convenient for the emergent transfer or transport of embryo；5. add by
After smart ovum, incubation can be intuitively observed, camera is installed, can dynamically observe incubation；When taking out observation in vitro, also not
The chemical factors in culture chamber can be changed, this characteristic is for extremely crucial for the embryo sensitive to environmental change；6. from embryo
When tire accommodating chamber takes embryo, when taking embryo as find still need to cultivate, embryo can be made to be back to Embryo Culture chamber and continue to cultivate, and
Embryo will not flow backward to sample intake passage during backflow；7. whole Embryo Culture unit good airproof performance, sample intake passage and sample output passage are adopted
With contraction type structure, and seamlessly transit, air exclusion is good；It is internal that 8. the preparation method of Embryo Culture unit solves processing
The problem of cavity lane, ensure interior smooth impulse- free robustness, and transitional region circular arc seamlessly transits.
Brief description of the drawings
Fig. 1 is Embryo Culture cell schematics of the present utility model.
Embodiment
The utility model is further clarified below in conjunction with the accompanying drawings.
Embodiment 1
Reference picture 1, a kind of Embryo Culture unit, include the culture unit main body 1 of transparent material, cultivate unit main body 1
Upper surface opens up a sample introduction end plug hole 3 and a sampling end consent 4, and plug is filled in sample introduction end plug hole 3 and sampling end consent 4
Sealing, the bottom in sample introduction end plug hole 3 set sample holes 5, and the bottom of sampling end consent 4 sets thief hole 6, thief hole 6 just under
Side sets embryo's accommodating chamber 7, sets Embryo Culture chamber 2 in culture unit main body 1, Embryo Culture chamber 2 is connected by sample intake passage 8
Sample holes 5, Embryo Culture chamber 2 connect embryo's accommodating chamber 7 by sample output passage 9.
In the present embodiment, culture unit main body 1 use glass material, can also be arbitrarily nontoxic to embryo saturating using other
Bright material, plug use silica gel plug, and silica gel plug elasticity is good, is easy to seal, and nontoxic to embryo.
In the present embodiment, the narrowest diameter of sample intake passage 8 is not more than 1.5mm, in actual processing, can be processed into diameter 1-
1.5mm circular hole.The most narrow place of sample intake passage 8 is located at upper end, and sample holes 5 gradually taper up, and seamlessly transits to sample intake passage 8 most
Narrow place, then sample intake passage 8 gradually amplify, the lower end of sample intake passage 8 connects with Embryo Culture chamber 2.The lower end connection of sample output passage 9
Embryo Culture chamber 2, sample output passage 9 gradually taper up, and the most narrow place channel sized of sample output passage 9 is 1.5-2.0mm, sample output passage 9
Most narrow place after again gradually amplification, connected using smooth arc transition with embryo's accommodating chamber 7.Because sample intake passage 8 is most narrow
Place is not more than 1.5mm, and the most narrow place channel sized of sample output passage 9 is 1.5-2.0mm, plays the speed for slowing down both sides fluid communication
Degree, be advantageous to the effect that culture chamber environment keeps stable, in sampling process is loaded, to the environment shadow of internal Embryo Culture chamber 2
Sound is very small, and the inside of the Embryo Culture unit is filled with advance using the good culture medium of carbon dioxide balance (it is recommended that using a journey
Formula culture medium), sealed by silica gel plug, in use, first then taking out sample introduction end plug hole in 37 DEG C of insulating box rewarmings about 45 minutes
3 silica gel plug, after being put into embryonated egg, silica gel plug is stoppered at once, the Embryo Culture unit is put into constant incubator, by
Smart ovum slowly spreads to Embryo Culture chamber 2.Because it is transparent material, Embryo Culture can be taken out in any desired time
Unit observes embryonic development situation, and camera dynamic observation Embryo Culture process can be also installed in constant incubator, has been observed
Finish, put back to and continue culture in insulating box；Such as need to take out embryo, stand up Embryo Culture unit, you can embryo is flowed into embryo
Accommodating chamber 7, then recover normal horizontally-arranged, open sampling plug, can pipe taking-up embryo using embryo transfer suction pipe；If it find that
Still need to further cultivate, handstand Embryo Culture unit, embryo is back in Embryo Culture chamber 2, then horizontally-arranged continuation back to normal
Culture, can also be transferred in another new Embryo Culture unit and continue to cultivate.
During in order to prevent from rotating Embryo Culture unit, embryo flows back into sample intake passage 8, sample intake passage 8 and Embryo Culture
The junction of chamber 2 is connected by one section of transition arc, and the bending direction of transition arc is curved with the sample intake passage wall of the junction
Qu Fangxiang and Embryo Culture cavity wall bending direction are on the contrary, the transition arc should be with sample intake passage 8 and the phase of tire tire culture chamber 2 certainly
Cut, to ensure that embryonated egg smoothly flows into Embryo Culture chamber 2.Change a kind of form of presentation, the lower end passage boring ratio embryo of sample intake passage 8
The hole of culture chamber 2 is small, and the junction of Embryo Culture chamber 2 and sample intake passage 8 passes through the transition of rapid desufflation section 10, rapid desufflation section 10
Playing prevents embryonated egg or embryo to be back to sample intake passage 8.
In the present embodiment, the bottom of Embryo Culture chamber 2 is curved, ensures in incubation, embryonated egg or embryo collect in
Together, and the lowest part of Embryo Culture chamber 2 is rested on.
In the present embodiment, the bottom of embryo's accommodating chamber 7 is curved, and the minimum point of embryo's accommodating chamber 7 is located at thief hole 6
Underface, conveniently take embryo.
In the present embodiment, from sample holes 5 to sample intake passage 8, then to Embryo Culture chamber 2, then to sample output passage 9, then to embryo
The whole vias inner walls of tire accommodating chamber 7 seamlessly transit, and without turning and burr, corner uses tangent circular arc
Transition so that embryonated egg or embryonic cell slidably, are not tangled.
In the present embodiment, multiple tracks transverse direction fin in the side wall of sample introduction end plug hole 3 and sampling end consent 4, screw thread can be used
Shape, or thread like fin, horizontal fin can extrude silica gel plug, play sealing effectiveness, ensure air tight.
In the present embodiment, the aperture outer of sample holes 5 is higher than the base plane in sample introduction end plug hole 3 so that aperture outer can
To squeeze into silica gel plug bottom surface, ensure sealing effectiveness.Similarly, the aperture outer of thief hole 6 is put down higher than the bottom of sampling end consent 4
Face.
In the present embodiment, a diameter of 10mm of sample introduction end plug hole 3 and sampling end consent 4.Sample holes 5 are diameter 4mm circles
Type, as long as sample holes 5 are easy to be put into embryonated egg, herein under the premise of, sample holes are 5 mouthfuls as far as possible small.Thief hole 6 is ellipse,
Because when taking embryo, probe tube is usually to tilt insertion embryo accommodating chamber 7, is observed for convenience in sampling, so taking
It is processed into ellipse in sample hole 6.Optimal, thief hole 6 is major axis 6mm, short axle 3mm ellipse.
In the present embodiment, the dimensions of culture unit main body 1 is long 55mm, wide 25mm, high 35mm.
In the present embodiment, the surface of culture unit main body 1 sets breach, and breach is used to fix elastic band, and elastic band is fixed
Silica gel plug, prevent silica gel plug from loosening, further, culture unit main body 1 upper surface sets the neck for placing silica gel plug, in order to
Fixed placement after extraction silica gel plug, does not mix up unrest or drops.
Load in advance with after the good formula culture medium (see the past utility model patent) of carbon dioxide balance, constitute one
The In vitro culture system of individual absolute tightness, embryonated egg ectogenesis can be supported to blastaea directly in 37 DEG C of common insulating boxs
Stage.For embryonated egg, this system has completely disengaged the use of CO2gas incubator, and observes embryo in confined conditions
Development, not only Embryo Culture stability is more preferable, quality is higher outer, moreover it is possible to significantly saves the space, a large amount of of Embryo Culture room
Reduce CO2Usage amount, significantly simplify incubation step, working strength and reduce operational error so as to considerably reduce
Probability, realize the safety for both protecting embryo and operating personnel, improve operating efficiency, work quality again and economize on resources
Purpose.
Embryo Culture unit of the present utility model, after adding embryonated egg, it can intuitively observe the growth course of embryo, installation
Camera, can dynamically it observe, using preceding without carbon dioxide balance, during culture and observation, internal physiological environment will not
Change, whole device small volume, light weight, can largely use.User keeping temperature need to only balance in constant incubator.
The utility model is illustrated by way of example above, but the utility model is not limited to above-mentioned specific implementation
Example, all scopes that the requires of the utility model protection is belonged to based on any changes or modifications that the utility model is done.
Claims (8)
1. a kind of Embryo Culture unit, it is characterized in that：Culture unit main body including transparent material, cultivate the upper table of unit main body
Face opens up a sample introduction end plug hole and a sampling end consent, fills in plug seal in sample introduction end plug hole and sampling end consent, enters
The bottom in sample end plug hole sets sample holes, and the bottom of sampling end consent sets thief hole, and the underface of thief hole sets embryo to hold
Receive chamber, Embryo Culture chamber is set in culture unit main body, Embryo Culture chamber passes through sample intake passage and connects sample holes, Embryo Culture chamber
Embryo's accommodating chamber is connected by sample output passage.
2. Embryo Culture unit according to claim 1, it is characterized in that：The most narrow place of sample intake passage is located at upper end, sample introduction
Hole gradually tapers up, and seamlessly transits to the most narrow place of sample intake passage, and then sample intake passage gradually amplifies, sample intake passage lower end and embryo
Culture chamber connects；The lower end connection Embryo Culture chamber of sample output passage, sample output passage gradually tapers up, after the most narrow place of sample output passage
Gradually amplification again, is connected using smooth arc transition with embryo's accommodating chamber.
3. Embryo Culture unit according to claim 2, it is characterized in that：The junction of Embryo Culture chamber and sample intake passage leads to
Cross quick contraction section transition.
4. Embryo Culture unit according to claim 2, it is characterized in that：The narrowest diameter of sample intake passage is not more than
1.5mm, most narrow place's channel diameter size of sample output passage is 1.5-2.0mm.
5. Embryo Culture unit according to claim 1, it is characterized in that：Unit main body is cultivated using nontoxic to embryo saturating
Bright material, plug use silica gel plug.
6. Embryo Culture unit according to claim 1, it is characterized in that：The bottom of Embryo Culture chamber is curved, and embryo holds
Receive chamber bottom it is curved, and the minimum point of embryo's accommodating chamber is located at the underface of thief hole.
7. Embryo Culture unit according to claim 1, it is characterized in that：In the side wall of sample introduction end plug hole and sampling end consent
Multiple tracks transverse direction fin, the aperture outer of sample holes are higher than the base plane in sample introduction end plug hole, and the aperture outer of thief hole, which is higher than, to be taken
The base plane in sample end plug hole.
8. Embryo Culture unit according to claim 1, it is characterized in that：Sample holes are diameter 4mm circular holes, and thief hole is
Major axis 6mm, short axle 3mm elliptical aperture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720608483.2U CN206858591U (en) | 2017-05-27 | 2017-05-27 | A kind of Embryo Culture unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720608483.2U CN206858591U (en) | 2017-05-27 | 2017-05-27 | A kind of Embryo Culture unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206858591U true CN206858591U (en) | 2018-01-09 |
Family
ID=60825892
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720608483.2U Expired - Fee Related CN206858591U (en) | 2017-05-27 | 2017-05-27 | A kind of Embryo Culture unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206858591U (en) |
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2017
- 2017-05-27 CN CN201720608483.2U patent/CN206858591U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180109 |