CN206848173U - A kind of fluorescence probe box for mercury ion detecting - Google Patents
A kind of fluorescence probe box for mercury ion detecting Download PDFInfo
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- CN206848173U CN206848173U CN201720760284.3U CN201720760284U CN206848173U CN 206848173 U CN206848173 U CN 206848173U CN 201720760284 U CN201720760284 U CN 201720760284U CN 206848173 U CN206848173 U CN 206848173U
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- mercury ion
- box
- fluorescence probe
- probe
- box body
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Abstract
It the utility model is related to mercury ion detecting apparatus field, more particularly to mercury ion quick detection probe box in a kind of environmental water sample, including box body, the instrument for mercury ion detecting is provided with the box body, the instrument includes the probe diaphragm chamber of the fluorescence probe polyacrylic film equipped with detection mercury ion, for filling cushioning liquid, alkaline pH regulation liquid, acid pH regulation liquid and the reagent bottle of mercury ion standard liquid, include carrying the microsyringe of the carrier of clip, scissors, quartz colorimetric utensil, at least one disposable plastic tube and some different sizes.The beneficial effects of the utility model：The utility model provides a kind of fluorescence probe box for mercury ion detecting, the probe box structure is simple, it is simple for production, cheap, be easy to carry about with one, deposit and use, the thin film testing mercury ion especially filled in probe box is more convenient, it is not necessary to professional testing staff, available for preliminary judgement.
Description
Technical field
It the utility model is related to mercury ion detecting apparatus field, and in particular to mercury ion quick detection in a kind of environmental water sample
Probe box.
Background technology
Heavy metal pollution have become in China one it is very important the problem of.Wherein, mercury is considered as most general in environment
All over one of existing toxic metals, animals and plants can be produced with the even fatal influence of injury.Injury to humans and animals is main
Central nervous system, digestive system, and internal organs are concentrated on, also have certain shadow to respiratory system, blood, skin and eyes
Ring.Influence to plant can cause its growth inhibition even dead.In addition, Hg2+With persistence, life easy to migrate and height
Thing enriching, this becomes one of environmental contaminants that the whole world is most noticeable at present.Therefore, the quantitative detection of mercury ion exists
Medicine, food, clinic and environment measuring etc. have very important meaning.It is typically conventional for the assay method of mercury at present
Have atomic absorption spectrum, atomic emission spectrum and inductive coupling plasma mass etc., but the equipment for using these methods
It is huge, and costliness is, it is necessary to which the pretreatment of complexity, measurement period is long and needs skilled professional operator, and this is in reality
Many inconveniences are brought in.Therefore, establish mercury in a set of practical, simple in construction, easily operated environmental water sample
Ion detection instrument turns into urgent problem to be solved.
Mercury ion can be acted on specifically with rhodamine fluorescence probe, and mercury ion detecting side can be established based on the principle
Method.Specific mercury ion rhodamine fluorescence probe is bonded on polyolefin acid film using the principle, has detection sensitive, accurate
Really, the characteristics of cheap, easy to use, have a good application prospect.
The content of the invention
The utility model in order to solve the assay method of current mercury ion used by equipment is huge, expensive and processing is multiple
A kind of the problems such as miscellaneous, there is provided fluorescence probe box for mercury ion detecting.
The utility model is achieved through the following technical solutions：A kind of fluorescence probe box for mercury ion detecting, bag
Box body is included, the instrument for mercury ion detecting is provided with the box body, the instrument includes the fluorescence equipped with detection mercury ion and visited
The probe diaphragm chamber of pin polyacrylic film, for filling cushioning liquid, alkaline pH regulation liquid, acid pH regulation liquid and mercury ion
The reagent bottle of standard liquid, in addition to the carrier with clip, scissors, quartz colorimetric utensil, at least one disposable plastic are inhaled
The microsyringe of pipe and some different sizes.
As the further improvement of technical solutions of the utility model, it is in box-packed base and lid that box body, which is included after mutually fastening,
Body.
As the further improvement of technical solutions of the utility model, the base and lid of box body are by hard transparent plastic system
Into.
As the further improvement of technical solutions of the utility model, the base and lid internal chamber wall of box body are provided with buffering foam
Plastic layer, buffering foam layer surface offer the groove for installing each instrument.
The beneficial effects of the utility model：The utility model provides a kind of fluorescence probe box for mercury ion detecting,
The probe box structure is simple, it is simple for production, cheap, be easy to carry about with one, deposit and use, the film especially filled in probe box
Detection mercury ion is more convenient, it is not necessary to professional testing staff, available for preliminary judgement.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the utility model, for those of ordinary skill in the art, is not paying the premise of creative work
Under, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is a kind of structural representation of fluorescence probe box for mercury ion detecting described in the utility model.
In figure：1- box bodys, 2- probe diaphragm chambers, 3- carriers, 4- scissors, 5- quartz colorimetric utensils, 6- disposable plastics are inhaled
Pipe, 7- microsyringes, 8,9,10,11- reagent bottles.
Embodiment
, below will be to technology of the present utility model to make the purpose of this utility model, technical scheme and advantage clearer
Scheme is described in detail.Obviously, described embodiment is only the utility model part of the embodiment, rather than all
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not before creative work is made
Resulting all other embodiment is put, belongs to the scope that the utility model is protected.
The technical solution of the utility model is described in detail below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of fluorescence probe box for mercury ion detecting, including box body 1, interior be provided with of the box body 1 are used
In the instrument of mercury ion detecting, the instrument includes the probe film of the fluorescence probe polyacrylic film equipped with detection mercury ion
Box 2, for fill cushioning liquid, alkaline pH regulation liquid, acid pH regulation liquid and mercury ion standard liquid reagent bottle 8,9,10,
11, in addition to carrier 3, scissors 4, quartz colorimetric utensil 5, at least one disposable plastic tube with clip（6）And if
The microsyringe 7 of dry different size.
The effect to each instrument and specification are described in detail below：
Fluorescence probe polyacrylic film of the described probe diaphragm chamber 2 equipped with detection mercury ion.Described film is handle
Rhodamine mercury ion fluorescence probe is connected on polyacrylic film.
The specification for being used to fill the reagent bottle 9,10,11 of pH regulation liquid and mercury ion standard liquid is 10 mL, is used for
The specification for filling the reagent bottle 8 of cushioning liquid is 100 mL, and material is polypropylene.
Buffer solution is the Tris-HCl buffer solutions of pH value 7.4, and concentration is 10 mM.
The pH regulation liquid reagent bottle 9,10 is respectively used to fill NaOH and hydrochloric acid, and the NaOH concentration of the pH regulations liquid is 1
M；Concentration of hydrochloric acid is 1 M.
The concentration of the mercury ion standard liquid is 50 mM.
The specification of the carrier 3 of the probe film is to grow 2.8 cm, and wide 0.8 cm, both ends are designed with clip, and material is equal
For transparent PVC.
The specification of the disposable plastic tube 6 is 3 mL, is equipped with 3-6, mounted on top.
The specification of the scissors 4 is long 6 cm, and material is stainless steel.
The specification of the microsyringe 7 is 5 μ L, 10 μ L, 50 μ L.
As shown in figure 1, easy to carry for the ease of testing staff, it is in box-packed bottom that the box body 1, which is included after mutually fastening,
Seat and lid.
When specifically used, the base and lid of box body 1 are made of hard transparent plastic.
In order to improve the stability and regularity of each instrument arrangement, the base and lid internal chamber wall of box body 1 are provided with buffering and steeped
Foam plastic layer, buffering foam layer surface offer the groove for installing each instrument.
Experimental principle（Application method i.e. of the present utility model）：The utility model is existed by fluorescence probe polyacrylic film
The concentration of mercury ion in fluorescence intensity change measuring environment water sample after being acted on mercury ion.Due to the complexity of environmental water sample,
PH is typically uncertain, so, weakly acidic pH is first transferred to, then, is adjusted sample near pH to 7.1 with pH regulations.Mercury ion is buffering
Had an effect in system with adding the fluorescence probe film of system, and send fluorescence.Utilize fluorescent spectrophotometer assay film
Fluorescence intensity level, by the fluorescence intensity level measured bring into calibration curve equation can in determination sample mercury ion concentration.By
When film is running into mercury ion, obvious color change can also occur, so, depositing for mercury ion in water sample can also be estimated
And content.
To further appreciate that technology contents, feature and effect of the present utility model, it is as follows to lift following examples detailed description.
（1）The making of standard curve：
Long 2.9 cm are taken with scissors 4, wide 0.9 cm films, is clipped on carrier 3, film is then soaked in various concentrations
Dimercurion Tris-HCl solution（10 mM）In, using 530 nm as excitation wavelength, measure film is in launch wavelength
Fluorescence intensity at 578 nm, is designated as F；And it is the fluorescence intensity at 578 nm to determine film blank in launch wavelength, is designated as F0,
Using the concentration C of mercury ion as abscissa, fluorescence intensity change value Δ F is ordinate, draws standard curve；
Wherein, Δ F=F-F0；A series of solution of the mercury ion of above-mentioned various concentrations detects mercury as described in claims
Mercury ion standard liquid in the fluorescence probe box of ion concentration dilutes and obtained；Dilution mercury ion is sampled with microsyringe 7
Concentration is followed successively by 0,1,3,6,10,30,60,90,120,150,180,200 μM.
（2）Detect the concentration of mercury ion in environmental water sample：
Water sample pre-treatment to be measured：Water sample to be measured is adjusted near pH to 7 with pH regulation liquid, buffer solution is then added, takes 3 mL
Into quartz colorimetric utensil 5.Then the immersion of probe film is put into quartz colorimetric utensil 5.
According to the step（1）Testing sample after methods described detection pre-treatment is at launch wavelength is 578 nm
Fluorescence intensity, F surveys are designated as, the F measuring tapes are entered into the step（1）Gained standard curve, obtain mercury ion in detected water sample
Concentration
The utility model low cost, economical and practical, simple production process, be advantageous to promote the use of.
Embodiment described above is merely to illustrate technological thought of the present utility model and feature, and its object is to make ability
Technical staff in domain is it will be appreciated that content of the present utility model and implement according to this, it is impossible to only limits this practicality with the present embodiment
New the scope of the claims, i.e., the equal change or modification that the spirit disclosed in all the utility model is made, still fall new in this practicality
In the scope of the claims of type.
Claims (4)
1. a kind of fluorescence probe box for mercury ion detecting, it is characterised in that including box body（1）, the box body（1）Inside it is provided with
For the instrument of mercury ion detecting, the probe that the instrument includes the fluorescence probe polyacrylic film equipped with detection mercury ion is thin
Bellows（2）, for filling cushioning liquid, alkaline pH regulation liquid, acid pH regulation liquid and the reagent bottle of mercury ion standard liquid（8、
9、10、11）, in addition to the carrier with clip（3）, scissors（4）, quartz colorimetric utensil（5）, at least one disposable plastic inhales
Pipe（6）And the microsyringe of some different sizes（7）.
A kind of 2. fluorescence probe box for mercury ion detecting according to claim 1, it is characterised in that box body（1）Bag
It is in box-packed base and lid to include after mutually fastening.
A kind of 3. fluorescence probe box for mercury ion detecting according to claim 2, it is characterised in that box body（1）'s
Base and lid are made of hard transparent plastic.
A kind of 4. fluorescence probe box for mercury ion detecting according to claim 3, it is characterised in that box body（1）'s
Base and lid internal chamber wall are provided with buffering foam layer, and buffering foam layer surface is offered for installing the recessed of each instrument
Groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720760284.3U CN206848173U (en) | 2017-06-28 | 2017-06-28 | A kind of fluorescence probe box for mercury ion detecting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720760284.3U CN206848173U (en) | 2017-06-28 | 2017-06-28 | A kind of fluorescence probe box for mercury ion detecting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206848173U true CN206848173U (en) | 2018-01-05 |
Family
ID=60802856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720760284.3U Expired - Fee Related CN206848173U (en) | 2017-06-28 | 2017-06-28 | A kind of fluorescence probe box for mercury ion detecting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206848173U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114280018A (en) * | 2021-12-17 | 2022-04-05 | 复旦大学 | Super-assembled fluorescent probe box for copper ion detection |
-
2017
- 2017-06-28 CN CN201720760284.3U patent/CN206848173U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114280018A (en) * | 2021-12-17 | 2022-04-05 | 复旦大学 | Super-assembled fluorescent probe box for copper ion detection |
| CN114280018B (en) * | 2021-12-17 | 2023-08-25 | 复旦大学 | A super equipment fluorescence probe box for copper ion detects |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180105 Termination date: 20180628 |