CN107271590A - A kind of method of headspace gas chromatography quick detection anti-bacteria paper biocidal property - Google Patents
A kind of method of headspace gas chromatography quick detection anti-bacteria paper biocidal property Download PDFInfo
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Abstract
The invention discloses a kind of method of headspace gas chromatography quick detection anti-bacteria paper biocidal property.A kind of method of Fast Evaluation anti-bacteria paper biocidal property is established using Headspace-Gas Chromatography, mainly reflect the extent of growth of bacterium by detecting the amount of carbon dioxide of the amount of oxygen consumed in defined incubation or generation, the biocidal property of anti-bacteria paper is evaluated so as to realize.As a result show：Be that 2.9 × 102CFU/mL, nutrient solution volume are the detection of optimum oxygen and carbon dioxide signals under conditions of 5mL, culture vessel are ml headspace bottle in 37 DEG C, incubation time 6h 8h, Escherichia coli bacteria liquid concentration, and can simplicity accurately calculate bacteriostasis rate value.This method substantially increases the efficiency of detection, is suitable for the Fast Evaluation to anti-bacteria paper biocidal property compared with conventional method (48h 72h).
Description
Technical field
The present invention relates to pulp and paper industry field, more particularly to a kind of headspace gas chromatography quick detection anti-bacteria paper are antibacterial
The method of property.
Background technology
Paper and paper products are a kind of and the closely related environmental protection of daily life, reproducible undulation degree
Material.With the paper for daily use of human contact (napkin paper, toilet paper, makeup removing paper, oil-Absorbing Sheets etc.), food wrapper, medicinal paper with
And bank note etc. can introduce harmful microorganism, such as during production, storage and use：Fungi, Pseudomonas aeruginosa, Escherichia coli
Deng easily causing courses of infection in use and cause the diseases such as enteritis, typhoid fever, dysentery.In addition, some are more valuable
The bacterium that the microorganism that the paper materials such as books, calligraphy and painting, security can be gradually stored during preservation, also in environment produces
(mainly mould) corrodes, and causes the heavy losses in terms of property.Therefore, research and development have antibacterial, antibacterial, sterilizing function
Paper product, it has also become one of focus of current new paper product exploitation.And the antibacterial of Fast Evaluation paper product or fungistatic effect
Detection method, the exploitation for antibacterial paper product is very important.
At present, paper antibacterial or the evaluation detection method of fungistatic effect are mainly inhibition zone method.This method is mainly used in surveying
Determine bacteriostasis of the bacteria mildew-proof agent in paper to bacterium, mould and saccharomycete, its principle is in agar using bacteriostatic agent
Diffusion makes the growth of the bacterium around it be suppressed and form transparent inhibition zone in plating medium, according to the big of inhibition zone
A kind of small method to judge the fungistatic effect of bacteria mildew-proof agent in paper.This method major defect is cumbersome, time-consuming (one
As need to cultivate bacterium on culture medium after 24h can just be measured), and qualitative or sxemiquantitative measure can only be carried out.
In order to overcome the defect that the degree of accuracy is not high, efficiency is low during traditional inhibition zone method evaluation fungistatic effect, some bases have been set up at present
In the analysis method of modern instrument, such as：Chemoluminescence method, atriphos (ATP) biloluminescence method etc., and portable biometric
The quick detection of the total number of bacteria of fluorescent optical sensor.However, these methods usually require that in sample bacterial concentration be more than 1000/
ML, to meet the requirement of detection sensitivity.At present also using fluorescent nanometer particle to mark method to multiple bacterium (such as mouse typhus sramana
Salmonella, staphylococcus aureus and Escherichia coli etc.) while fast qualitative detection is realized, but this method cannot be used for bacterial population
The quantitative analysis of amount.
Headspace gas chromatography detection technique is a kind of automatic modern equipment analytical technology, and it can be realized to complicated sample
Volatile component in product solution carries out large batch of efficient measure.Headspace gas is just utilized early in Gardinia in 1997 et al.
The yield of chromatography determination microbial metabolic products --- carbon dioxide monitors the cytoactive and fertility of microorganism.
However, the evaluation method for anti-bacteria paper biocidal property is still based on traditional counting method.Can be accurate therefore, it is necessary to develop one kind
The method of true Fast Evaluation anti-bacteria paper biocidal property.
The content of the invention
It is an object of the invention to the shortcoming and defect for overcoming above-mentioned prior art, there is provided a kind of headspace gas chromatography is quick
The method for detecting anti-bacteria paper biocidal property.It can reflect the growing state of bacterium by determining carbon dioxide and oxygen signal value,
Its content height, and then the easy bacteriostasis rate for calculating anti-bacteria paper exactly can be replaced with two kinds of gas signal values.In addition, also
Semi-automatic measure can be carried out to large batch of anti-bacteria paper.
Due to Escherichia coli in the presence of oxygen, aerobic respiration is carried out, oxygen is consumed and discharges carbon dioxide.
Shown in the net reaction of Escherichia coli aerobic respiration such as formula (1).
C6H12O6+6O2+6H2O→6CO2+12H2O+ energy (1)
According to carbon dioxide of the Escherichia coli in growth course to consumption and the generation of oxygen, both signals are determined
Value, the bacteriostasis rate of anti-bacteria paper can be calculated according to both variable quantities.I.e.：
In formula, AdThe peak area of carbon dioxide or oxygen in meteorology in airtight bottle, A are detected for control groupsDetected for experimental group
In airtight bottle in meteorology carbon dioxide or oxygen peak area, A0For in blank detection airtight bottle in gas phase initial carbon dioxide or
The peak area of oxygen.
The present invention is achieved through the following technical solutions：
The method of headspace gas chromatography quick detection anti-bacteria paper biocidal property, comprises the following steps：
Step (1)：Sample preparation：
Prepare anti-bacteria paper：Chitosan acetic acid solution is dissolved, silver nitrate solution is added, tune pH is alkalescent, suction filtration system
Obtain chitosan gel rubber.Chitosan gel rubber is coated on its surface anti-bacteria paper is made；
Prepare LB liquid and solid medium：Tryptone, yeast extract, sodium chloride are weighed, distilled water stirring is added
Dissolving, adjusts pH value for 7.0 (± 0.2) with sodium hydroxide solution, fluid nutrient medium is made after autoclaving.Aforesaid liquid is taken to train
Support base addition agar and heat to form it into and solid medium is made after solution, autoclaving.
Prepare the Escherichia coli bacteria liquid of various concentrations：Escherichia coli bacteria liquid culture certain time purchased in market is first taken, afterwards by bacterium
Liquid dilutes, and obtains the bacterium solution of various concentrations, and low dense bacterium solution is counted.
Step (2)：The culture of bacterium solution：
The bacterium solution and anti-bacteria paper of various concentrations are fitted into ml headspace bottle to be placed in constant incubator cultivating certain time, and set
Put control group (ordinary filter paper).
Step (3)：Sample detection：
With oxygen and carbon dioxide signals value in headspace gas chromatography detection ml headspace bottle.
Step (4)：Interpretation of result：
The oxygen and carbon dioxide signals value image of step (3) bacterium solution generation are made, experimental group and the oxygen of control group is utilized
Gas goes out the bacteriostasis rate of anti-bacteria paper with carbon dioxide mathematic interpolation.
Acetic acid chitosan concentration is 0.1%~1.0% in above-mentioned steps (1), and dissolution time is 1h~6h, is prepared anti-
The chitosan coating weight of bacterium paper is 0~15%；It is 3~3 × 10 to prepare Escherichia coli bacteria liquid concentration7CFU/mL。
Above-mentioned steps (2) condition of culture：The μ L of bacterium solution 10~1000,1~10mL of nutrient solution, pattern 0.01 in ml headspace bottle~
0.5g, 25~45 DEG C, 2h-20h.
Above-mentioned steps (3) the head-space sampler operating condition is：40~80 DEG C of equilibrium temperature, Sample equilibration time 4~
Carrier gas 10~20s of equilibration time, pipeline 10~20s of inflationtime, pipeline 1~10s of equilibration time in 40min, headspace sample bottle,
10~20s of loop balance time.
Above-mentioned steps (3) the gas chromatograph operating condition is：Chromatographic column temperature is 50~150 DEG C, and nitrogen is used as carrier gas.
Wherein, 2.0~6.0mL/min of nitrogen flow.
Above-mentioned steps (3) the gas chromatograph operating condition is：Chromatographic column temperature be 50~150 DEG C, nitrogen as carrier gas,
Nitrogen flow is 2.0~6.0mL/min；150~250 DEG C of TCD detector temperatures.
The computational methods of above-mentioned steps (4) interpretation of result anti-bacteria paper bacteriostasis rate have:Represented with oxygen signal value；With titanium dioxide
Carbon signal value is represented；Carbon dioxide and oxygen signal value changes amount ratio Analysis.
The present invention has the following advantages and effect relative to prior art：
First, when determining anti-bacteria paper biocidal property using this method, the obturation effect of ml headspace bottle is good, it is to avoid by miscellaneous bacteria
Pollution.
Secondly, this method does not need artificial counting bacterium, while Instrumental Analysis accuracy rate is high, sensitivity is good；Again can be short
Substantial amounts of detection is carried out in time.
Therefore, the biocidal property of anti-bacteria paper is detected using this method, the time is not only saved, improves detection efficiency, and can be with
Simplicity calculates bacteriostasis rate exactly.
Brief description of the drawings
Fig. 1 is the bacteriostasis rate (w calculated with carbon dioxideb) to the graph of a relation of the relative addition of silver nitrate
Embodiment
The present invention is more specifically described in detail with reference to specific embodiment.
Embodiment
Used instrument and equipment and reagent：Coating machine, drying machine, high-pressure steam sterilizing pan, biochemical cultivation case, shaking table,
Superclean bench, balance (0.001g), pH meter, liquid-transfering gun and pipette tips, Agilent A7890 types gas chromatograph (thermal conductivity detector (TCD),
HP-6890 types capillary chromatographic column), ml headspace bottle；
Chitosan, Escherichia coli (E.coli-ATCC), tryptone, yeast extract, agar powder, sulfuric acid (2mol/L),
Glacial acetic acid, silver nitrate, sodium chloride, sodium hydroxide.
(1) sample preparation：
Prepare anti-bacteria paper：1g chitosans are dissolved in 0.1% acetic acid solution, a certain amount of silver nitrate solution are added (with nitric acid
Silver is defined to the mass fraction of chitosan), adjust pH value to be 7.5 after 6h, chitosan gel rubber is made in suction filtration.Chitosan gel rubber is coated with
Anti-bacteria paper (chitosan 5%) is made in its surface；Repeat the above steps, with mass fraction of the different silver nitrates to chitosan
(table 1) prepares 10 anti-bacteria papers.
The anti-bacteria paper of table 1
Prepare LB liquid and solid medium：10g tryptones, 5g yeast extracts, 10gNaCL are weighed, is added
1000mL distilled water stirring and dissolvings, are 7.0 (± 0.2) with 1mol/L NaOH regulation pH value, autoclaving (121 DEG C of temperature, when
Between 15 minutes, 0.1MPa) be made fluid nutrient medium；Taking aforesaid liquid culture medium 500mL to add 7.5g agar and heat makes its shape
Into solution, solid medium is made in autoclaving (121 DEG C of temperature, 15 minutes time, 0.1MPa).
Pipetted on superclean bench with liquid-transfering gun in the liquid medium that 200 μ L strain Escherichia colis sterilize to 10mL,
Sealing, is put into culture 12h (37 DEG C, 150r/min) in shaking table and obtains Escherichia coli bacteria suspension.1mL bacteria suspensions are pipetted in test tube 1
In, 9mL sterile salines are added, is well mixed and is made 1：10 dilution；Same method is made 1：102、1：103、1：104、
1：105、1：106、1：107Bacterium dilution.Bacteria suspension, 1 are taken respectively：10、1：103、1：105、1：107Bacterium dilution conduct
1#、2#、3#、4#、5#Bacterium night to be measured.Bacterium night to be measured is inoculated in LB solid mediums, 24h is cultivated in 37 DEG C of constant incubators,
Bacterial concentration (table 2) is determined afterwards.
The bacterial concentration of table 2
(2) culture of bacterium solution：
The mass ratio of pattern and nutrient solution is 1%.Anti-bacteria paper is cut into the 1cm × 2cm scraps of paper, 5mL is pipetted with liquid-transfering gun
Nutrient solution weighs scraps of paper 0.05g, and pipette the μ L of bacterium night liquid 100 to be measured as experimental group in ml headspace bottle.Control group takes filter paper paper
Piece (1cm × 2cm) 0.05g, 5mL nutrient solution, the μ L of bacterium night 100 to be measured.Ml headspace bottle gland is sealed, is put into shaking table and cultivates 6h
(37 DEG C), taking-up is put into -4 DEG C of refrigerator and freezed.
(3) sample detection：
After the sample of freezing is thawed at room temperature, 20min in 80 DEG C of water-bath is put into.Then headspace gas chromatography is used
CO in instrument detection ml headspace bottle2And O2Signal value.
(4) result is calculated：
The oxygen and carbon dioxide signals value detected by step (3), the bacteriostasis rate of anti-bacteria paper can be calculated according to formula (2).
(5) measurement result
The anti-bacteria paper bacteriostasis rate of table 3
* for actual silver nitrate quality divided by coating needed for chitosan mass percentage；
**O2And CO2The signal value of blank is respectively 84.6,1.8.
According to table 3, the bacteriostasis rate (w calculated with carbon dioxide is drawnb) to the graph of a relation (figure of the relative addition of silver nitrate
1), its rule can be described by below equation, i.e.,：
wa=7.20*lnC+76.40
(3)
wb=11.24*lnC+72.22 (4)
In formula, waFor the bacteriostasis rate (%) represented with oxygen signal value, wbIt is antibacterial for what is represented with carbon dioxide signals value
Rate, C is the relative amount (%) of silver nitrate.
The w in table 3aAnd wbData understand, the variation tendency of the anti-bacteria paper bacteriostasis rate calculated with carbon dioxide and with oxygen
Unanimously.But when silver nitrate relative amount reaches 15%, the bacteriostasis rate w calculated with carbon dioxidebAlready close to 100%, and
The bacteriostasis rate w calculated with oxygenaFor 96%.This be due to bacterium after culture is started, can carry out aerobic respiration first and consume
Oxygen, and the inhibitory action of anti-bacteria paper has hysteresis quality, so waAnd wbThere is certain difference.Therefore, for high antibacterial material
Material, it is more objective with the bacteriostasis rate that carbon dioxide is calculated.
Fig. 1 is the bacteriostasis rate (w calculated with this batch of anti-bacteria paper with carbon dioxideb) to the relation of the relative addition of silver nitrate
Figure.As seen from the figure, with the gradually increase of silver nitrate content, first quick increase, rear slow rise is presented in the bacteriostasis rate of anti-bacteria paper
Trend, it can also be seen that from the consideration of economical rationality from Fig. 1, silver nitrate is 1.2% to be advisable with respect to addition, its antibacterial
The bacteriostasis rate of paper can reach 75.0%.
As described above, the present invention can be better realized.
Embodiments of the present invention are simultaneously not restricted to the described embodiments, other any Spirit Essences without departing from the present invention
With the change made under principle, modification, replacement, combine, simplify, should be equivalent substitute mode, be included in the present invention
Within protection domain.
Claims (9)
1. a kind of method of headspace gas chromatography quick detection anti-bacteria paper biocidal property, it is characterised in that by determining oxygen and two
Carbonoxide signal value reflects the growing state of bacterium indirectly, then calculates the bacteriostasis rate of anti-bacteria paper；Comprise the following steps：
Step (1)：Sample preparation：
Prepare anti-bacteria paper：Chitosan acetic acid solution is dissolved, tune pH is alkalescent, chitosan gel rubber is made in suction filtration；Shell is gathered
Anti-bacteria paper is made in its surface in sugared gel coating；
Prepare LB liquid and solid medium：Tryptone, yeast extract, sodium chloride, add distilled water stirring and dissolving, use hydrogen
Sodium hydroxide solution regulation pH value is obtained fluid nutrient medium after neutrality, autoclaving；Aforesaid liquid culture medium is taken to add agar simultaneously
Heating, which is formed it into, is made solid medium after solution, autoclaving；
Prepare the Escherichia coli bacteria liquid of various concentrations：First take Escherichia coli bacteria liquid purchased in market and cultivated, afterwards dilute bacterium solution,
The bacterium solution of various concentrations is obtained, and low dense bacterium solution is counted；
Step (2)：The culture of bacterium solution：
The bacterium solution and anti-bacteria paper of various concentrations are fitted into ml headspace bottle to be placed in being cultivated in constant incubator, and control is set
Group；
Step (3)：Sample detection：
With oxygen and carbon dioxide signals value in headspace gas chromatography detection ml headspace bottle；
Step (4)：Interpretation of result：
Make oxygen and the carbon dioxide signals value image of step (3) bacterium solution generation, using experimental group and control group oxygen with
Carbon dioxide mathematic interpolation goes out the bacteriostasis rate of anti-bacteria paper.
2. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 1, it is characterised in that：Step
(1) acetic acid chitosan concentration is 0.1%~1.0% in, and dissolution time is 1h~6h, prepares the chitosan coating of anti-bacteria paper
Measure as 0~15%；
The Escherichia coli bacteria liquid concentration for preparing is 3~3 × 107CFU/mL。
3. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 1, it is characterised in that：Step
(2) the μ L of bacterium solution 10~1000,1~10mL of nutrient solution, 0.01~0.5g of pattern in ml headspace bottle.
4. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 1, it is characterised in that：Step
(2) condition of culture：25~45 DEG C, 2h-24h.
5. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 1, it is characterised in that：Step
(3) the head-space sampler operating condition is：40~80 DEG C of equilibrium temperature, 4~40min of Sample equilibration time, headspace sample bottle
10~20s of middle carrier gas equilibration time, pipeline 10~20s of inflationtime, pipeline 1~10s of equilibration time, the loop balance time 10~
20s。
6. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 5, it is characterised in that step
(3) the gas chromatograph operating condition is：Chromatographic column temperature is 50~150 DEG C, and nitrogen is used as carrier gas.
7. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 6, it is characterised in that：It is described
Nitrogen as carrier gas, wherein, 2.0~6.0mL/min of nitrogen flow.
8. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 7, it is characterised in that：Step
(3) in the gas chromatograph operating condition, 150~250 DEG C of TCD detector temperatures.
9. the method for headspace gas chromatography quick detection anti-bacteria paper biocidal property according to claim 1, it is characterised in that：Step
(4) computational methods of interpretation of result anti-bacteria paper bacteriostasis rate have:Represented with oxygen signal value；Represented with carbon dioxide signals value；Two
Carbonoxide and oxygen signal value changes amount ratio Analysis.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398496A (en) * | 2018-02-09 | 2018-08-14 | 中国科学院武汉植物园 | A kind of natural products Determination of Antibacterial Activity method |
| CN110068632A (en) * | 2019-05-22 | 2019-07-30 | 华南理工大学 | Method based on headspace gas chromatography measurement chitosan derivatives amino content |
| CN111239274A (en) * | 2020-01-22 | 2020-06-05 | 广西大学 | Method for measuring apparent yield coefficient of aerobic activated sludge |
| CN111337587A (en) * | 2020-02-10 | 2020-06-26 | 广西大学 | Method for high-flux determination of soil microbial biomass |
| CN111363781A (en) * | 2020-03-05 | 2020-07-03 | 中国科学院武汉植物园 | Method for measuring antibacterial activity of natural product on anaerobic bacteria and application thereof |
| CN113866302A (en) * | 2021-09-26 | 2021-12-31 | 青岛海关技术中心 | Detection method for rapidly distinguishing commercial sterility of canned fruits |
| CN114019036A (en) * | 2021-09-26 | 2022-02-08 | 青岛海关技术中心 | Detection method for rapidly distinguishing commercial sterility of cubilose cans |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2240556C2 (en) * | 2002-11-10 | 2004-11-20 | Кировская государственная медицинская академия | Method of determining antimicrobial activity of vegetable oil in vitro |
| JP4953071B2 (en) * | 2007-04-02 | 2012-06-13 | 独立行政法人産業技術総合研究所 | Method for evaluating antibacterial performance of photocatalytic materials against general environmental microorganisms |
| CN102586395A (en) * | 2012-03-06 | 2012-07-18 | 中华人民共和国吴江出入境检验检疫局 | Plating medium-based leather material bacteriostasis effect test method |
| CN102586396A (en) * | 2012-03-06 | 2012-07-18 | 中华人民共和国吴江出入境检验检疫局 | Cylinder plate method-based leather material bacteriostasis effect test method |
| CN103792259A (en) * | 2014-02-21 | 2014-05-14 | 中国人民解放军第三〇二医院 | Method for evaluating antimicrobial activity of andrographis paniculata |
| CN104419745A (en) * | 2013-09-11 | 2015-03-18 | 广东省东莞市质量监督检测中心 | Paper product bacterial colony detection method |
-
2017
- 2017-05-11 CN CN201710329696.6A patent/CN107271590A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2240556C2 (en) * | 2002-11-10 | 2004-11-20 | Кировская государственная медицинская академия | Method of determining antimicrobial activity of vegetable oil in vitro |
| JP4953071B2 (en) * | 2007-04-02 | 2012-06-13 | 独立行政法人産業技術総合研究所 | Method for evaluating antibacterial performance of photocatalytic materials against general environmental microorganisms |
| CN102586395A (en) * | 2012-03-06 | 2012-07-18 | 中华人民共和国吴江出入境检验检疫局 | Plating medium-based leather material bacteriostasis effect test method |
| CN102586396A (en) * | 2012-03-06 | 2012-07-18 | 中华人民共和国吴江出入境检验检疫局 | Cylinder plate method-based leather material bacteriostasis effect test method |
| CN104419745A (en) * | 2013-09-11 | 2015-03-18 | 广东省东莞市质量监督检测中心 | Paper product bacterial colony detection method |
| CN103792259A (en) * | 2014-02-21 | 2014-05-14 | 中国人民解放军第三〇二医院 | Method for evaluating antimicrobial activity of andrographis paniculata |
Non-Patent Citations (2)
| Title |
|---|
| 李靖等: "三唑类杀菌剂的合成工艺及其抑菌性评价", 《木材工业》 * |
| 田迎新等: "顶空气相色谱法快速检测生活用纸中的大肠菌群", 《中国造纸》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398496A (en) * | 2018-02-09 | 2018-08-14 | 中国科学院武汉植物园 | A kind of natural products Determination of Antibacterial Activity method |
| CN110068632A (en) * | 2019-05-22 | 2019-07-30 | 华南理工大学 | Method based on headspace gas chromatography measurement chitosan derivatives amino content |
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| CN111337587A (en) * | 2020-02-10 | 2020-06-26 | 广西大学 | Method for high-flux determination of soil microbial biomass |
| CN111363781A (en) * | 2020-03-05 | 2020-07-03 | 中国科学院武汉植物园 | Method for measuring antibacterial activity of natural product on anaerobic bacteria and application thereof |
| CN111363781B (en) * | 2020-03-05 | 2023-12-29 | 中国科学院武汉植物园 | Method for measuring antibacterial activity of natural product on anaerobic bacteria and application thereof |
| CN113866302A (en) * | 2021-09-26 | 2021-12-31 | 青岛海关技术中心 | Detection method for rapidly distinguishing commercial sterility of canned fruits |
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