CN114280018B - A super equipment fluorescence probe box for copper ion detects - Google Patents
A super equipment fluorescence probe box for copper ion detects Download PDFInfo
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- CN114280018B CN114280018B CN202111548708.7A CN202111548708A CN114280018B CN 114280018 B CN114280018 B CN 114280018B CN 202111548708 A CN202111548708 A CN 202111548708A CN 114280018 B CN114280018 B CN 114280018B
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Abstract
The invention belongs to the field of copper ion detection equipment, and provides a super-assembled fluorescent probe box for copper ion detection. The detection tool comprises a probe film box containing a PMOs-PAN film probe, a buffer solution reagent bottle containing a buffer solution, an acid pH regulator reagent bottle containing an acid pH regulator, an alkaline pH regulator reagent bottle containing an alkaline pH regulator and a copper ion standard solution reagent bottle containing a copper ion standard solution. The fluorescent probe box is convenient to use, simple in structure, simple and convenient to manufacture, low in price, easy to carry and use, more convenient for detecting copper ions by PMOs-PAN in the probe box, has long-term light stability, and can be used for preliminary judgment.
Description
Technical Field
The invention belongs to the field of copper ion detection equipment, and particularly relates to a super-assembled fluorescent probe box for copper ion detection.
Background
With the progress of industrialization, heavy metal pollution has become a serious environmental problem threatening human health and environmental ecology. Copper is used as heavy metal, and is also essential trace element for animals, plants and human beings, and trace copper can promote the growth of animals, plants, but when a certain amount of copper is accumulated in organisms, the copper can cause victimization symptoms, physiological obstruction, development arrest and even death. In addition Cu 2+ Is widely used in the fields of energy, construction, electric power and the like, and excessive Cu from industrial waste and domestic sewage in the environment 2+ In order to reduce the harm of heavy metal ions to the environment and human beings, therefore, the quantitative detection of copper ions has very important practical significance in the fields of environment, medical treatment, food safety and the like. The current method for detecting copper ions is generally extraction separation detection, spectrophotometry detection, atomic absorption spectrometry detection, fluorescence spectrometry and the like, but the operation of adopting the methods is complex, the design is huge, the method is expensive, very complex pretreatment is needed, and professional detection personnel are needed, which brings about very high actual detectionThe method is inconvenient, so that the establishment of a set of practical, simple-structure and easy-to-operate copper ion detection tool for an environmental water sample is a problem to be solved at present.
Disclosure of Invention
The invention provides a fluorescent probe box for rapidly detecting copper ions, which aims to solve the problems of complex method for detecting copper ions, huge and expensive adopted equipment, complex treatment and the like.
The invention provides a fluorescent probe box for rapidly detecting copper ions, which has the characteristics that: a case body; the detection tool is placed in the box body; wherein the detection tool comprises: a probe film box containing a PMOs-PAN film probe; a buffer solution reagent bottle containing a buffer solution; an acidic pH regulator reagent bottle containing an acidic pH regulator; an alkaline pH regulator reagent bottle containing an alkaline pH regulator; the reagent bottle for the copper ion standard solution is filled with the copper ion standard solution.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: the preparation method of the PMOs-PAN thin film probe comprises the following steps: and embedding the triaminoguanidine-benzenediol serving as a conjugate into mesoporous silica spheres synthesized by a double-interface method to obtain an organic-inorganic hybrid mesoporous silica material, and then mixing the organic-inorganic hybrid mesoporous silica material with polyacrylonitrile for electrostatic spinning to prepare the PMOs-PAN thin film probe.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: wherein the buffer solution is PBS buffer solution with pH value of 7.4 and concentration of 0.1M.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: wherein, the alkaline pH regulator is borax solution with the concentration of 0.01M; the acidic pH regulator is a potassium hydrogen phthalate solution with the concentration of 0.05M.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: wherein the concentration of the copper ion standard solution is 0.01M.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the characteristics that the fluorescent probe box further comprises: ultraviolet lamp flashlight, tweezers, disposable plastic straw, black opaque slide glass and centrifuging tube.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: the ultraviolet lamp flashlight is 365nm ultraviolet lamp flashlight; the length of the tweezers is 8cm, and the tweezers are made of stainless steel.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: wherein, the bottle bodies of the buffer solution reagent bottle, the acid pH regulator reagent bottle, the alkaline pH regulator reagent bottle and the copper ion standard solution reagent bottle are all made of glass materials, and the bottle caps are made of polypropylene materials.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: the box body comprises a base and a box cover which are in a box shape after being buckled with each other, and the base and the box cover are both made of hard black plastics.
The fluorescent probe box for rapidly detecting copper ions provided by the invention can also have the following characteristics: wherein, the inner chamber wall of base and lid is equipped with the buffering foam layer, and a plurality of recesses that are used for splendid attire instrument are seted up on buffering foam layer surface.
Effects and effects of the invention
According to the fluorescent probe box for copper ion detection, provided by the invention, the probe film box is provided with the PMOs-PAN film probe, the PMOs-PAN film probe can sensitively detect copper ions, the detection of copper ions is more convenient, the fluorescent probe box has long-term light stability, no professional detection personnel is needed, and the fluorescent probe box can be used for preliminary judgment.
The probe box has the characteristics of sensitive and accurate detection, simple structure, convenient manufacture, low price, easy carrying, storage and use, repeated use and the like, and has good application prospect.
Drawings
Fig. 1 is a schematic structural view of a fluorescent probe cartridge in an embodiment of the present invention.
Reference numerals illustrate: 1-a box body; 2-tweezers; 3-probe film box; 4-copper ion standard solution reagent bottle; 5-an acidic pH regulator reagent bottle; 6-an alkaline pH regulator reagent bottle; 7-a buffer solution reagent bottle; 8-a disposable plastic straw; 9-ultraviolet flashlight; 10-5ml centrifuge tube; 11-glass slide.
Detailed Description
In order to make the technical means, creation characteristics, achievement of the purposes and effects of the present invention easy to understand, a fluorescent probe box for copper ion rapid detection according to the present invention will be specifically described with reference to the following examples and the accompanying drawings.
The structures, proportions, sizes, etc. shown in the drawings attached hereto are for illustration purposes only and are not intended to limit the scope of the invention, which is defined by the claims, but rather by the claims. Also, the terms such as "upper," "lower," "left," "right," "middle," and "a" and the like recited in the present specification are merely for descriptive purposes and are not intended to limit the scope of the invention, but are intended to provide relative positional changes or modifications without materially altering the technical context in which the invention may be practiced.
< example >
The present embodiment specifically describes a structure and a method of using a fluorescent probe box for rapid detection of copper ions (hereinafter referred to as fluorescent probe box).
Fig. 1 is a schematic structural view of a fluorescent probe cartridge in an embodiment of the present invention.
As shown in fig. 1, the fluorescent probe box comprises a box body 1, a detection tool for detecting copper ions and other auxiliary tools, wherein the detection tool is placed in the box body 1.
In order to facilitate the carrying of the detection box, the box body 1 comprises a base and a box cover which are mutually buckled to form a box. When in specific use, the base and the box cover of the box body 1 are made of hard black plastic. The base size is 25cm long by 20cm by 10cm, the cover size is 25cm long by 20cm by 5cm.
In order to prevent the detection tool and other auxiliary tools from running up and down and left and right in the box body 1, the base of the box body 1 and the inner cavity wall of the box cover are provided with buffer foam plastic layers, and grooves for installing the tools are formed in the surfaces of the buffer foam plastic layers.
The detection tool comprises a probe film box 3, a copper ion standard solution reagent bottle 4, an acid pH regulator reagent bottle 5, an alkaline pH regulator reagent bottle 6 and a buffer solution reagent bottle 7.
And the probe film box 3 is filled with a PMOs-PAN film probe capable of sensitively detecting copper ions. The preparation method of the PMOs-PAN thin film probe comprises the following steps:
and embedding the triaminoguanidine-benzenediol serving as a conjugate into mesoporous silica pellets synthesized by a double-interface method to obtain an organic-inorganic hybrid mesoporous silica material, and mixing the organic-inorganic hybrid mesoporous silica material with polyacrylonitrile for electrostatic spinning to prepare the PMOs-PAN thin film probe. The specific preparation process of the PMOs-PAN thin film probe by mixing and electrostatic spinning of the organic-inorganic hybrid mesoporous silica material and polyacrylonitrile is as follows:
firstly, dispersing 0.01g-0.1g of organic-inorganic hybrid mesoporous silica material in 10ml-100ml of dimethylacetamide solution, slowly adding 0.02g-0.4g of polyacrylonitrile powder into the powder mixed solution, stirring for about 20min-60min until the mixture is fully swelled by a glass rod, transferring the solution into a round bottom flask, heating in a constant-temperature oil bath pot with the temperature of 40-100 ℃ and refluxing for condensation, stirring by an electric stirrer, and taking out for standby after 2-4 h. And then injecting 5ml-10ml of the obtained solution into a syringe, regulating the voltage of an electrostatic spinning instrument to 5KV-20KV, starting electrostatic spinning, after spinning for 4h-12h, putting the film obtained under a silk collector into a baking oven at 30-60 ℃ for drying, and obtaining the PMOs-PAN film probe.
The copper ion standard solution reagent bottle 4 contains copper ion standard solution. The acid pH regulator reagent bottle 5 is filled with an acid pH regulator; the alkaline pH adjuster reagent bottle 6 contains an alkaline pH adjuster. The buffer solution reagent bottle 7 contains a buffer solution.
The copper ion standard solution reagent bottle 4, the acid pH regulator reagent bottle 5, the alkaline pH regulator reagent bottle 6 and the buffer solution reagent bottle 7 are all made of glass, and the bottle caps are made of polypropylene. The specifications of the copper ion standard solution reagent bottle 4, the acid pH regulator reagent bottle 5 and the alkaline pH regulator reagent bottle 6 are 10mL, and the specification of the buffer solution reagent bottle 7 for containing the buffer solution is 50mL.
The buffer solution was PBS buffer at pH 7.4 at a concentration of 0.1M. The alkaline pH regulator is borax solution with concentration of 0.01M. The acidic pH regulator is a potassium hydrogen phthalate solution with the concentration of 0.05M. The concentration of the copper ion standard solution was 0.01M.
Other auxiliary tools include an ultraviolet lamp flashlight 9, tweezers 2, a plurality of disposable plastic straws 8, a plurality of black opaque glass slides 11 and a plurality of centrifuge tubes 10. Also included are carriers with clips, scissors (not shown).
The outer lamp flashlight is a 365nm ultraviolet lamp flashlight. The length of the forceps 2 is 8cm, and the forceps are made of stainless steel. The disposable plastic suction pipe 8 has the specification of 3mL, is provided with 3 to 5 pieces and is stacked up and down. Centrifuge tube 10 is 5ml in size.
The fluorescent probe box changes the fluorescence intensity after the PMOs-PAN film reacts with copper ions so as to achieve the effect of measuring the concentration of the copper ions. Because of the complexity of environmental water samples, the pH is generally uncertain, so the pH of the water sample is adjusted to about 7.4 by using a pH regulator. Copper ions react with PMOs-PAN film probes added into the buffer system to quench the film which emits yellow light under an ultraviolet lamp. Because the quenching degree is different, compared with the quenching degree of the standard solution of copper ions on the film, the existence and the approximate content of the copper ions in the water sample can be visually detected, the quenched water sample is sucked by a centrifuge tube, and the quenched water sample is brought back and the content of the copper ions in the water sample is measured by a fluorescence gradiometer in detail.
The specific operation is described in detail as follows:
pretreatment of a water sample to be tested: and (3) sucking a water sample to be detected into a centrifuge tube, regulating the pH to be near 7.4 by using a pH regulating solution, then adding a buffer solution, clamping a film onto a black glass slide by using tweezers, sucking the regulated water sample to drop onto the film, irradiating the glass slide by using a 365nm ultraviolet flashlight, and comparing according to the quenching degree of yellow fluorescence of the film and the quenching degree of standard copper ions to drop onto the film to observe whether copper ions exist in the water sample and the approximate concentration of the copper ions. If the concentration of copper ions is found to be out of standard, a 5ml centrifuge tube is utilized to absorb the quenched water sample, the quenched water sample is brought back, and the content of copper ions in the water sample is measured in detail by a fluorescence gradiometer.
Effects and effects of the examples
The fluorescent probe box for copper ion detection provided by the embodiment is provided with a probe film box containing a PMOs-PAN film probe. The synthesized triaminoguanidine-benzenediol conjugate is embedded into mesoporous silica pellets synthesized by a double-interface method, and the obtained organic-inorganic hybrid mesoporous silica material (PMOs) can perform specific chelation with copper ions to cause the material to perform fluorescence quenching. Based on this principle, a detection method of copper ions can be established. The PMOs and polyacrylonitrile are mixed and electrospun to prepare the fluorescent sensing film (PMOs-PAN) by utilizing the principle, and the fluorescent sensing film has the characteristics of sensitive and accurate detection, low price, reusability, convenient use and the like, and has good application prospect.
The PMOs-PAN thin film probe in the detection box has the advantages of simple structure, convenient manufacture, low price, easy carrying, storage and use, capability of sensitively detecting copper ions, more convenience in detecting copper ions, long-term light stability, no need of professional detection personnel and capability of being used for preliminary judgment.
While the foregoing embodiments have been described in connection with the accompanying drawings, it is not intended to limit the scope of the invention, and it will be apparent to those skilled in the art that various modifications or variations can be made without inventive effort within the scope of the invention.
Claims (9)
1. A fluorescent probe box for rapid detection of copper ions, comprising:
a case body; and
a detection tool disposed within the cartridge;
wherein the detection tool comprises:
a probe film box containing a PMOs-PAN film probe;
a buffer solution reagent bottle containing a buffer solution;
an acidic pH regulator reagent bottle containing an acidic pH regulator;
an alkaline pH regulator reagent bottle containing an alkaline pH regulator; and
a reagent bottle for copper ion standard solution, which is filled with copper ion standard solution,
the preparation method of the PMOs-PAN thin film probe comprises the following steps: embedding triaminoguanidine-benzenediol as conjugate into mesoporous silica spheres synthesized by a double-interface method to obtain an organic-inorganic hybrid mesoporous silica material, mixing the organic-inorganic hybrid mesoporous silica material with polyacrylonitrile, carrying out electrostatic spinning to prepare the PMOs-PAN thin film probe,
the specific preparation process of the PMOs-PAN thin film probe by mixing and electrostatic spinning the organic-inorganic hybrid mesoporous silica material and polyacrylonitrile is as follows:
dispersing the organic-inorganic hybrid mesoporous silica material of 0.01-g-0.1-g in 10-100 ml of dimethylacetamide solution, adding 0.02-g-0.4-g polyacrylonitrile powder, stirring until the mixture is fully swelled, transferring the solution into a round bottom flask, placing the round bottom flask into a constant-temperature oil bath pot with the temperature of 40-100 ℃ for heating, refluxing and condensing, stirring, taking out the solution after 2-h-4-h, carrying out electrostatic spinning on the obtained solution by an electrostatic spinning instrument, after 4-h-12 h of spinning, putting a film obtained under a silk collector into an oven with the temperature of 30-60 ℃ for drying, and obtaining the PMOs-PAN film probe.
2. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, wherein:
wherein the buffer solution is PBS buffer solution with pH value of 7.4 and concentration of 0.1M.
3. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, wherein:
wherein the alkaline pH regulator is borax solution with the concentration of 0.01M;
the acidic pH regulator is potassium hydrogen phthalate solution with the concentration of 0.05M.
4. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, wherein:
wherein the concentration of the copper ion standard solution is 0.01M.
5. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, further comprising:
ultraviolet lamp flashlight, tweezers, disposable plastic straw, black opaque slide glass and centrifuging tube.
6. The fluorescent probe cartridge for rapid detection of copper ions according to claim 5, wherein:
the ultraviolet lamp flashlight is 365nm ultraviolet lamp flashlight;
the tweezers are 8-cm in length and made of stainless steel.
7. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, wherein:
the buffer solution reagent bottle, the acid pH regulator reagent bottle, the alkaline pH regulator reagent bottle and the copper ion standard solution reagent bottle are all made of glass, and the bottle caps are made of polypropylene.
8. The fluorescent probe cartridge for rapid detection of copper ions according to claim 1, wherein:
the box body comprises a base and a box cover which are buckled with each other to form a box, and the base and the box cover are made of hard black plastic.
9. The fluorescent probe cartridge for rapid detection of copper ions according to claim 8, wherein:
the inner cavity walls of the base and the box cover are provided with buffer foam plastic layers, and a plurality of grooves for containing the detection tools are formed in the surfaces of the buffer foam plastic layers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111548708.7A CN114280018B (en) | 2021-12-17 | 2021-12-17 | A super equipment fluorescence probe box for copper ion detects |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111548708.7A CN114280018B (en) | 2021-12-17 | 2021-12-17 | A super equipment fluorescence probe box for copper ion detects |
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| CN114280018A CN114280018A (en) | 2022-04-05 |
| CN114280018B true CN114280018B (en) | 2023-08-25 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106497544A (en) * | 2016-10-20 | 2017-03-15 | 上海电力学院 | A kind of organic photochromic material based on diarylethene and its preparation method and application |
| CN206848173U (en) * | 2017-06-28 | 2018-01-05 | 山西大同大学 | A kind of fluorescence probe box for mercury ion detecting |
| CN111285897A (en) * | 2020-03-03 | 2020-06-16 | 江苏海洋大学 | Mesoporous silicon fluorescent probe for copper ion detection and separation and preparation method thereof |
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2021
- 2021-12-17 CN CN202111548708.7A patent/CN114280018B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106497544A (en) * | 2016-10-20 | 2017-03-15 | 上海电力学院 | A kind of organic photochromic material based on diarylethene and its preparation method and application |
| CN206848173U (en) * | 2017-06-28 | 2018-01-05 | 山西大同大学 | A kind of fluorescence probe box for mercury ion detecting |
| CN111285897A (en) * | 2020-03-03 | 2020-06-16 | 江苏海洋大学 | Mesoporous silicon fluorescent probe for copper ion detection and separation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Highly sensitive and selective Cu 2+ sensor based on electrospun rhodamine dye doped poly(ether sulfones) nanofibers;Minghua Min;Sensors and Actuators B: Chemical;全文 * |
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| CN114280018A (en) | 2022-04-05 |
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