CN206706093U - A kind of lid has the PCR pipe of sample collection function concurrently - Google Patents
A kind of lid has the PCR pipe of sample collection function concurrently Download PDFInfo
- Publication number
- CN206706093U CN206706093U CN201720546113.0U CN201720546113U CN206706093U CN 206706093 U CN206706093 U CN 206706093U CN 201720546113 U CN201720546113 U CN 201720546113U CN 206706093 U CN206706093 U CN 206706093U
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- Prior art keywords
- lid
- action bars
- sample collection
- collection function
- groove
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- 230000009471 action Effects 0.000 claims abstract description 55
- 230000007246 mechanism Effects 0.000 claims abstract description 21
- 230000001681 protective effect Effects 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 4
- 238000007493 shaping process Methods 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 16
- 239000012535 impurity Substances 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 4
- 238000003780 insertion Methods 0.000 abstract description 2
- 230000037431 insertion Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 12
- 238000009434 installation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- GQWNECFJGBQMBO-UHFFFAOYSA-N Molindone hydrochloride Chemical compound Cl.O=C1C=2C(CC)=C(C)NC=2CCC1CN1CCOCC1 GQWNECFJGBQMBO-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000218636 Thuja Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses the PCR pipe that a kind of lid has sample collection function concurrently, body including sealed bottom, the lid being arranged above body and the internal action bars for forming cavity, the lower surface of the lid is shaped to mat surface, the action bars can be socketed on the lid, operating mechanism is provided with the internal cavities of the action bars, to be detachably connected, the position that the side wall of the action bars corresponds to the operating mechanism offers operation window for the lid and the operating mechanism.The utility model is directly acquired using action bars to sample cell；After collection terminates, by the operating mechanism set in action bars internal cavities, the lid insertion with sample cell is equipped with the body of PCR reaction solutions and releases the connection with action bars, sample cell is directly directly contacted with PCR reaction solutions and is reacted；The processing time to sample can be so saved, reduces the introducing of impurity in sample again, it is convenient and swift.
Description
Technical field
It the utility model is related to a kind of Bioexperiment instrument, and in particular to a kind of lid has the PCR of sample collection function concurrently
Pipe.
Background technology
1985, the polymerase chain that Perkin-Elmer Cetus companies of U.S. utility models has an epoch-marking significance was anti-
Answer (Polymerase Chain Reaction, PCR), make the hope for the external unlimited amplification of nucleic acid fragment that people dream of
Become a reality.Round pcr is similar to DNA natural reproduction process, and its specificity depends on the few core complementary with target sequence both ends
Thuja acid primer.PCR is made up of denaturation-fundamental reaction step of annealing-extension three.This course of reaction is in PCR reaction vessels
Middle progress.With the continuous development of gene-amplificative instrament, its reaction vessel also experienced from 1.5ml to 0.2ml (0.25ml) from
Heart pipe progressively develops into the 0.2ml of the special thin-walleds of PCR, 0.1ml PCR pipe.With the raising of pcr amplification reaction quantity, by
Step forms the high-throughout gene magnification container of PCR bars, PCR plate.During early stage PCR, it is stated that the design of drum lid can more preferable vaporization prevention.
But later hot lid PCR instrument occurs, flat cover PCR pipe is often applicable, drum lid is just not easy to use heat lid, and this mainly sees PCR
Instrument.Present PCR instrument has hot lid, but is divided into flat, two kinds of drum, corresponds to two kinds of PCR pipes respectively, supports the use preferably.
But be all to need to sample this cell first in existing PCR reactions, then artificially sample cell is added again
Into PCR reaction solutions, the complex steps that operate and being also easy to make to be mixed into impurity in the sample of question response, and then have influence on
The result of PCR reactions.
Utility model content
In order to solve the problems such as above-mentioned existing PCR operations are cumbersome, question response sample easily pollutes, the utility model carries
A kind of lid has been supplied to have the PCR pipe of sample collection function concurrently, concrete scheme is as follows：
A kind of lid has the PCR pipe of sample collection function, including the body of sealed bottom, the internal lid for forming cavity concurrently
Body and the internal action bars for forming cavity, the inwall of the body form some steps, and the lower end of the lid is shaped to
The structure coordinated with the inboard wall of tube body step, the side and lower surface of the lid are shaped to mat surface；The action bars
One end is socketed on the lid, and operating mechanism, the lid and the operation machine are provided with the internal cavities of the action bars
For structure to be detachably connected, the position that the side wall of the action bars corresponds to the operating mechanism offers operation window.
Further, the operating mechanism includes the fixed mount for being fixed on the action bars internal cavities vertically, described solid
Determine the central shaft that frame passes through the action bars, be equipped with horizontally disposed motion bar on the fixed mount, connect on the motion bar
Drive link straight down is connected to, the bottom of the drive link is provided with limited block, and the motion bar is located at the fixed mount and institute
The part stated between drive link is arranged with elastomeric spring；The motion bar stretches out against one end of operation window from the operation window,
The motion bar, which is stretched out on one end of the operation window, is fixed with operation button；The lid upper end, which upwardly extends shaping, fixation
Sleeve pipe, fixed sleeving are input in action bars internal cavities, and the inwall of the fixed sleeving forms can be with the limited block
The fixed groove of cooperation, is provided with some catch in the fixed groove, the limited block be deep into the fixed groove it is interior and with
The fixed groove overlap joint.
Further, the depth of the fixed groove is less than or equal to the spacing block edge to the drive link bottom
Distance, ensure that limited block successfully can be deviate from from fixed groove.
Further, the width of the fixed groove is more than the thickness of the limited block, limited block is swimmingly entered
Enter or remove fixed groove.
Further, the operating mechanism includes being fixedly connected on the first annular circle of the action bars inwall, and described the
The upper surface of one annular ring forms annular groove, is provided with the second annular ring in the annular groove, the first annular circle with
The upper surface of second annular ring is respectively equipped with the first pin and the second pin, on first pin and second pin
It is equipped with card；The position of the corresponding operation window of first annular circle offers the breach connected with the annular groove,
The connection sheet stretched out by the breach from the operation window is connected with the side wall of second annular ring；Outside the lid
Wall opens up fluted close to the position on top.
Further, the card forming is the shape of isosceles triangle, the card and first pin and described
The tie point of second pin is two end points on the card base.
Further, the diameter of the first annular circle endoporus is more than the diameter of lid, ensures that lid can be in the first ring
Passed through in shape circle；The thickness of the card is less than the width of the groove, ensures that card can swimmingly enter or remove groove.
Further, the screw thread of mutual cooperation is formed on the outer wall of the lid and the inwall of body.
Further, one end of the disconnected lid of the action bars is connected with some sizes and increases and connect together successively
Telescopic tube.
Further, protective cap is socketed with outside one end that the action bars is connected with the lid, prevents action bars from existing
There is impurity to enter in the cavity of action bars in the state of.
The beneficial effects of the utility model are：The utility model proposes a kind of lid have sample collection function concurrently
PCR pipe, lid of the bottom with mat surface is connected in the cavity of action bars, then utilizes the behaviour with certain stretch capability
Make bar to be directly acquired sample cell；After collection terminates, by the operating mechanism set in action bars internal cavities, by band
Have in body of the lid insertion equipped with PCR reaction solutions of sample cell and release the connection with action bars, make sample cell direct
Directly contact and reacted with PCR reaction solutions；The processing time to sample can be so saved, reduces impurity in sample again
Introduce, it is convenient and swift.
Brief description of the drawings
The structural representation of the body of Fig. 1 the utility model embodiments 1,
The sectional view of the body of Fig. 2 the utility model embodiments 1,
The structural representation of the lid of Fig. 3 the utility model embodiments 1,
The sectional view of the lid of Fig. 4 the utility model embodiments 1,
The structural representation of the action bars working end of Fig. 5 the utility model embodiments 1,
The structural representation of the operating mechanism of Fig. 6 the utility model embodiments 1,
The scheme of installation of Fig. 7 the utility model embodiments 1,
Structural representation under the action bars working end off working state of Fig. 8 the utility model embodiments 1,
The structure top view of the operating mechanism of Fig. 9 the utility model embodiments 2,
The scheme of installation of the first annular circle of Figure 10 the utility model embodiments 2 and the second annular ring,
The sectional view of the lid of Figure 11 the utility model embodiments 2,
The scheme of installation of Figure 12 the utility model embodiments 2.
Figure number and title：1st, body, 101, step, 2, lid, 201, mat surface, 202, fixed sleeving, 203, solid
Determine groove, 204, catch, 205, groove, 3, action bars, 301, operation window, 302, fixed mount, 303, motion bar, 304, transmission
Bar, 305, limited block, 306, elastomeric spring, 307, operation button, 308, first annular circle, the 309, second annular ring, 310,
One pin, the 311, second pin, 312, card, 313, breach, 314, connection sheet, 4, protective cap.
Embodiment
To describe technology contents, construction feature, institute's reached purpose and effect of the utility model in detail, reality is hereby enumerated below
Apply example and coordinate accompanying drawing to be explained in detail.
In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", "front", "rear",
The orientation of the instruction such as "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer ", " upper end ", " lower end " or position are closed
It is to be described based on orientation shown in the drawings or position relationship, to be for only for ease of description the utility model and simplifying, rather than
Indicate or imply that signified device or element there must be specific orientation, with specific azimuth configuration and operation, therefore can not
It is interpreted as the limitation to scope of protection of the utility model.
Embodiment 1
Referring to shown in Fig. 1-Fig. 5, the utility model provides the PCR pipe that a kind of lid has sample collection function concurrently, including
The body 1 of sealed bottom, the internal lid 2 for forming cavity and the internal action bars 3 for forming cavity, the inwall of body 1 into
Type has step 101；The lower end of lid 2 is shaped on the structure coordinated with the inwall step of body 1 and the inwall of its outer wall and body 1
The screw thread of mutual cooperation is formed, the lower surface of lid 2 is shaped to mat surface 201；One end of action bars 3 is socketed on lid 2,
Operating mechanism is provided with the internal cavities of action bars 3, for lid 2 with operating mechanism to be detachably connected, the side wall of action bars 3 is corresponding
The position of operating mechanism offers operation window 301.
As shown in fig. 6, operating mechanism includes the fixed mount 302 for being fixed on the internal cavities of action bars 3 vertically, fixed mount 302
Through the central shaft of action bars 3, horizontally disposed motion bar 303 is equipped with fixed mount 302, is connected with motion bar 303 perpendicular
Straight downward drive link 304, the bottom of drive link 304 is provided with limited block 305；Motion bar 303 is located at fixed mount 302 and drive link
Part between 304 is arranged with elastomeric spring 306；Motion bar 303 stretches out against one end of operation window 301 from operation window 301, living
Lever 303, which is stretched out on one end of operation window 301, is fixed with operation button 307；The upper end of lid 2, which upwardly extends shaping, fixed sleeving
202, fixed sleeving 202 is input in the internal cavities of action bars 3, and the inwall of fixed sleeving 202 forms can be with limited block 305
The fixed groove 203 of cooperation, fixed groove 203 is interior to be provided with some catch 204, and limited block 305 can be deep into fixed groove 203
Interior and overlapped with fixed groove 203, the width of fixed groove 203 is more than the thickness of limited block 305, the depth of fixed groove 203
Distance less than or equal to the edge of limited block 305 to drive link bottom, ensure that limited block 305 can swimmingly enter or remove solid
Determine groove.
One end of 3 disconnected lid of action bars is connected with the telescopic tube that some sizes increase and connected together successively,
The length of action bars 3 is adjusted according to use demand；As shown in figure 8, outside one end that action bars is connected with lid
Portion is socketed with protective cap, prevents action bars from having impurity to enter in the cavity of action bars in the state of.
From the point of view of Fig. 7, the course of work of embodiment 1 is as follows：First, action bars is extended to by telescopic tube specified
Length, the protective cap on removal operation bar；Then press the operation button, drive limited block to be moved to the left by drive link, will cover
One end of body fixed sleeving, which is stretched into the cavity inside action bars, makes fixed groove be directed at limited block, operation button is unclamped, in bullet
Limited block is caught in fixed groove in the presence of property spring；Now operator can directly utilize the lid being connected on action bars
The collection of target cell is carried out, sample cell is attached on the mat surface of lid bottom, then by the lid with target cell
Body is inserted directly into the body equipped with PCR reaction solutions and rotated to an angle and lid is screwed on body using screw thread；Finally,
Operation button is clicked again, and limited block is deviate from fixed groove, completes the separation of action bars and lid, makes the sample on mat surface
This cell directly contacts with body PCR reaction solutions to be reacted.The processing time to sample can be so saved, reduces sample again
The introducing of impurity in this.
Embodiment 2
Refer to shown in Fig. 2 to Fig. 5, the difference of embodiment 2 and embodiment 1 is：Operating mechanism includes being fixedly connected on
The first annular circle 308 of action bars inwall, the upper surface of first annular circle 308 form annular groove, are provided with annular groove
Second annular ring 309, the diameter of 308 endoporus of first annular circle are more than the diameter of lid 2, the first annular annular of circle 308 and second
The upper surface of circle 309 is respectively equipped with the first pin 310 and the second pin 311.
The first annular annular ring 309 of circle 308 and second is provided with the card 312 for being shaped as isosceles triangle, the bottom of card 312
Two end points on side are arranged on the first pin 310 and the second pin 311 respectively；308 respective operations windows 301 of first annular circle
Position offers the breach 313 connected with annular groove, is connected with the side wall of the second annular ring 309 by breach 313 from behaviour
Make the connection sheet 314 of the stretching of window 301；The outer wall of lid 2 opens up fluted 205 close to the position on top, and the width of groove 205 is big
In the thickness of card.
From the point of view of Figure 12, the course of work of embodiment 2 is as follows：First, action bars is extended to by finger by telescopic tube
Measured length, the protective cap on removal operation bar；Then connection sheet is stirred to the right to limit on the right-right-hand limit position, and now card is located at the first ring
The top of shape circle, lid one end with groove, which is stretched into the cavity inside action bars, makes groove be located at same level with card
Face；Connection sheet is subsequently stirred to the left to limit on the left position, now in the groove of the tip screw-in lid of card, that is, completes lid
Fixation；Now operator can directly carry out the collection of target cell using action bars, sample cell is attached to lid bottom
On the mat surface in portion, then the lid with target cell is inserted directly into the body equipped with PCR reaction solutions and rotated certain
Lid is screwed on body by angle using screw thread；Finally, connection sheet is stirred to the right to limit on the right-right-hand limit position, and card takes off in groove
Go out, complete the separation of action bars and lid, the sample cell on mat surface is directly contacted progress with body PCR reaction solutions anti-
Should.The processing time to sample can be so saved, reduces the introducing of impurity in sample again.
In summary, the only preferred embodiment of the utility model, does not limit the scope of protection of the utility model with this, all
The equivalent changes and modifications made according to the utility model patent scope and description, it is all what the utility model patent covered
Within the scope of.
Claims (10)
1. a kind of lid has the PCR pipe of sample collection function concurrently, including the body (1) of sealed bottom, it is arranged above body (1)
Lid (2) and the internal action bars (3) for forming cavity, it is characterised in that：The inwall of the body (1) forms some
Rank (101), the lower end of lid (2) are shaped to the structure coordinated with body (1) inwall step, and the side of the lid (2) is with
End face is shaped to mat surface (201)；One end of the action bars (3) is socketed on the lid (2), the action bars (3)
Operating mechanism is provided with internal cavities, the lid (2) is to be detachably connected with the operating mechanism, the action bars (3)
The position that side wall corresponds to the operating mechanism offers operation window (301).
2. a kind of lid according to claim 1 has the PCR pipe of sample collection function concurrently, it is characterised in that：The operation
Mechanism includes being fixed on the fixed mount (302) of the action bars (3) internal cavities vertically, and the fixed mount (302) is described in
The central shaft of action bars (3), horizontally disposed motion bar (303), the motion bar are equipped with the fixed mount (302)
(303) drive link (304) straight down is connected with, the bottom of the drive link (304) is provided with limited block (305), described
Part of the motion bar (303) between the fixed mount (302) and the drive link (304) is arranged with elastomeric spring (306)；
The motion bar (303) is stretched out against one end of operation window from the operation window (301), described in motion bar (303) stretching
Operation button (307) is fixed with one end of operation window (301)；Upper end upwardly extends shaping to the lid (2) fixed sleeving
(202), fixed sleeving (202) is input in action bars (3) internal cavities, and the inwall of the fixed sleeving (202) forms admittedly
Determine groove (203), be provided with some catch (204) in the fixed groove (203), the limited block (305) is deep into described solid
Determine to overlap in groove (203) and with the fixed groove (203).
3. a kind of lid according to claim 2 has the PCR pipe of sample collection function concurrently, it is characterised in that：The fixation
The depth of groove (203) is less than or equal to the limited block (305) edge to the distance of the drive link (304) bottom.
4. a kind of lid according to claim 2 has the PCR pipe of sample collection function concurrently, it is characterised in that：The fixation
The width of groove (203) is more than the thickness of the limited block (305).
5. a kind of lid according to claim 1 has the PCR pipe of sample collection function concurrently, it is characterised in that：The operation
Mechanism includes being fixedly connected on the first annular circle (308) of the action bars (3) inwall, the first annular circle (308) it is upper
Surface forming has annular groove, and the second annular ring (309), the first annular circle (308) and institute are provided with the annular groove
The upper surface for stating the second annular ring (309) is respectively equipped with the first pin (310) and the second pin (311), first pin
(310) and on second pin (311) it is equipped with card (312)；The corresponding operation window of first annular circle (308)
(301) position offers the breach (313) connected with the annular groove, connects in the side wall of second annular ring (309)
It is connected to the connection sheet (314) stretched out by the breach (313) from the operation window (301)；The outer wall of the lid (2) is close
The position on top opens up fluted (205).
6. a kind of lid according to claim 5 has the PCR pipe of sample collection function concurrently, it is characterised in that：The card
(312) shape of isosceles triangle, the card (312) and first pin (310) and second pin are shaped to
(311) tie point is two end points on the card (312) base.
7. a kind of lid according to claim 5 has the PCR pipe of sample collection function concurrently, it is characterised in that：Described first
The diameter of annular ring (308) endoporus is more than the diameter of the lid (2), and the thickness of the card (312) is less than the groove
(205) width.
8. a kind of lid according to claim 1 has the PCR pipe of sample collection function concurrently, it is characterised in that：The lid
(2) screw thread of mutual cooperation is formed on outer wall and the inwall of body (1).
9. a kind of lid according to claim 1-8 any one has the PCR pipe of sample collection function concurrently, its feature exists
In：One end of the disconnected lid of the action bars (3) is connected with the telescopic tube that some sizes increase and connected together successively.
10. a kind of lid according to claim 9 has the PCR pipe of sample collection function concurrently, it is characterised in that：The operation
Protective cap (4) is socketed with outside one end that bar (3) is connected with the lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720546113.0U CN206706093U (en) | 2017-05-16 | 2017-05-16 | A kind of lid has the PCR pipe of sample collection function concurrently |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720546113.0U CN206706093U (en) | 2017-05-16 | 2017-05-16 | A kind of lid has the PCR pipe of sample collection function concurrently |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206706093U true CN206706093U (en) | 2017-12-05 |
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ID=60464344
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720546113.0U Expired - Fee Related CN206706093U (en) | 2017-05-16 | 2017-05-16 | A kind of lid has the PCR pipe of sample collection function concurrently |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206706093U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741766A (en) * | 2019-12-13 | 2020-02-04 | 徐文博 | Device for cultivating plant seeds to germinate |
-
2017
- 2017-05-16 CN CN201720546113.0U patent/CN206706093U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110741766A (en) * | 2019-12-13 | 2020-02-04 | 徐文博 | Device for cultivating plant seeds to germinate |
| CN110741766B (en) * | 2019-12-13 | 2022-09-13 | 徐文博 | Device for cultivating plant seeds to germinate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210506 Address after: 100088 2201, building 8, yard 1, Xinfeng street, Xicheng District, Beijing Patentee after: Xing Jiangfeng Address before: 100032 room 318, North Great Wall optoelectronic building, 36 Hetaoyuan West Street, Xicheng District, Beijing Patentee before: BEIJING YILI TECHNOLOGY DEVELOPMENT Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171205 |
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| CF01 | Termination of patent right due to non-payment of annual fee |