CN206696297U - A kind of liquid-adding device for Western blotting - Google Patents
A kind of liquid-adding device for Western blotting Download PDFInfo
- Publication number
- CN206696297U CN206696297U CN201720304742.2U CN201720304742U CN206696297U CN 206696297 U CN206696297 U CN 206696297U CN 201720304742 U CN201720304742 U CN 201720304742U CN 206696297 U CN206696297 U CN 206696297U
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- China
- Prior art keywords
- liquid
- adding device
- delivery port
- feeding
- fluid filling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000001262 western blot Methods 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 18
- 239000012530 fluid Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 12
- 239000004372 Polyvinyl alcohol Chemical class 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- 229920002451 polyvinyl alcohol Chemical class 0.000 claims description 6
- -1 poly ethylene Polymers 0.000 claims description 4
- JWYVGKFDLWWQJX-UHFFFAOYSA-N 1-ethenylazepan-2-one Chemical class C=CN1CCCCCC1=O JWYVGKFDLWWQJX-UHFFFAOYSA-N 0.000 claims description 3
- 150000001298 alcohols Chemical class 0.000 claims description 3
- 229920002635 polyurethane Polymers 0.000 claims description 3
- 239000004814 polyurethane Substances 0.000 claims description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 23
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000001179 sorption measurement Methods 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 29
- 239000010408 film Substances 0.000 description 11
- 230000035611 feeding Effects 0.000 description 8
- 239000002699 waste material Substances 0.000 description 6
- 230000002209 hydrophobic effect Effects 0.000 description 5
- 229940068984 polyvinyl alcohol Drugs 0.000 description 4
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical class C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of liquid-adding device for Western blotting.The liquid-adding device has one or more water inlets and multiple delivery ports, and the material of the delivery port is water wetted material, with adsorption reaction liquid, is shaped as circular or ellipse, the internal diameter of the delivery port is 0.1mm~2mm, and external diameter is 0.5mm~5mm.Liquid-adding device of the present utility model can not only to Western blotting film add reaction solution, its hydrophilic delivery port can also adsorption reaction liquid, make reaction solution along strip hydrophilic region move back and forth, so as to improve reaction efficiency, save the time of immune-blotting method；Meanwhile the hydrophilic delivery port of liquid-adding device, moreover it is possible to adsorption reaction liquid, prevent spilling for reaction solution so that immune-blotting method only needs the reaction solution below milliliter level, so as to more save reagent, suitable for trace detection.
Description
Technical field
The utility model belongs to protein analysis field, more particularly, to a kind of liquid-adding device for Western blotting.
Background technology
Western blotting (Western Blot) technology is by Protein transfer to film, is then detected using antibody
Technology.Western blotting is usually used in identifying certain albumen, and can carry out quantitative and semi-quantitative analysis to albumen.Examined with reference to chemiluminescence
Survey, can simultaneously more multiple sample same proteins expression difference.Because Western blotting has SDS-PAGE high-resolution
The high specific and sensitiveness of power and solid-phase immunoassay, as a kind of routine techniques of analysis of protein.
In order to realize efficient automated analysis, various immunoblotting assay instrument should need and give birth to (patent document
CN103616526A).However, above-mentioned immunoblotting assay instrument generally carries out multiple treating test sample using multiple reagent bottles or reactive tank
This Parallel testing, while vibrated reagent bottle using shaking table, to accelerate reaction speed.Therefore using the film have with
Lower shortcoming：1st, due to being integrated with shaking table and motor for oscillating agents bottle so that whole analyzer structure is complex, and then
Improve manufacturing cost；2nd, the reaction condition of different reagent bottles slightly has difference, so as to influence the accuracy of Western blotting；3rd, it is thin
Film needs to be dried and can just be detected after taking out from reagent bottle, so as to which in situ detection can not be realized, result in there is
Underaction or hyperreactive situation, cause detection error.
Utility model content
For the disadvantages described above or Improvement requirement of prior art, the utility model provides a kind of adding for Western blotting
Liquid device, its object is to simplify the structure of automatic detection device while realize in situ detection.
To achieve the above object, according to one side of the present utility model, there is provided a kind of for the automatic of Western blotting
Detector, including base, Western blotting film, liquid-adding device and waste collecting device；
The Western blotting film is arranged above base, and the Western blotting film surface has with hydrophobic region interval
Multiple parallel strip hydrophilic regions, the width of the strip hydrophilic region is 1mm~5mm, and it has one or more tie
Area is closed, the liquid-adding device has and strip hydrophilic region multiple liquid feeding passages correspondingly, the water outlet of the liquid feeding passage
Mouth is oppositely arranged with corresponding strip hydrophilic region, the water inlet of one end connection waste collecting device of the strip hydrophilic region
Mouthful；
Wherein, there is target antigen, for being combined with the target antibody in solution to be measured on the land；It is each described
Strip hydrophilic region is respectively used to combine different solution to be measured, so as to which the target independently carried out in different solution to be measured resists
The immune-blotting method of body；The liquid-adding device is used to add reaction solution to strip hydrophilic region, and the waste collecting device is used
In the unnecessary reaction solution of collection；The reaction solution includes solution to be measured, buffer solution, two corresponding anti-solution or chromophoric solution.
According to other side of the present utility model, a kind of liquid-adding device for above-mentioned automatic detector is additionally provided,
The liquid-adding device has one or more water inlets and multiple delivery ports, and the material of the delivery port is water wetted material, with
Adsorption reaction liquid, circular or ellipse being shaped as, the internal diameter of the delivery port is 0.1mm~2mm, and external diameter is 0.5mm~5mm,
It is adapted with the width with strip hydrophilic region.
Preferably, the liquid-adding device includes multiple liquid-feeding tubes and fluid filling pump, and the fluid filling pump has and multiple liquid feedings
Multiple delivery ports corresponding to pipe, each delivery port of the fluid filling pump are connected with the water inlet of multiple liquid-feeding tubes, are formed multiple
Liquid feeding passage, the water inlet of the water inlet of the fluid filling pump as the liquid-adding device, the delivery port of the liquid-feeding tube is as institute
State the delivery port of liquid-adding device.
As it is further preferred that the fluid filling pump is syringe pump or peristaltic pump.
As it is further preferred that the peristaltic pump is flow type peristaltic pump.
As it is further preferred that the liquid-adding device also includes fix bar, the fix bar has multiple fixed parts, uses
It is fixed in by multiple liquid-feeding tubes on same straight line.
As it is further preferred that the fixed part is the fixing hole corresponding with the liquid-feeding tube, the fixing hole
It is sheathed on the periphery of the liquid-feeding tube.
As it is further preferred that the liquid-feeding tube is water wetted material.
Preferably, the delivery port of the liquid-adding device has a hydrophilic coating, the hydrophilic coating be polyvinylpyrrolidone,
Poly N-vinyl butyrolactam, poly-N-vinylcaprolactam, polyvinyl alcohol, polyvinyl alcohol derivative, polyurethane or modified poly-
Ethene.
In general, by the contemplated above technical scheme of the utility model compared with prior art, due to automatic
Liquid-adding device has been used in detector, following beneficial effect can be obtained：
1st, liquid-adding device can not only add reaction solution to Western blotting film, and its hydrophilic delivery port can also adsorption reaction
Liquid, reaction solution is moved back and forth along strip hydrophilic region using the surface tension of water, so as to improve reaction efficiency, save immune
The time of trace detection；
2nd, the hydrophilic delivery port of liquid-adding device, moreover it is possible to adsorption reaction liquid, prevent spilling for reaction solution so that Western blotting
Detection only needs the reaction solution below milliliter level, so as to more save reagent, suitable for trace detection；
3rd, can ensure multiple liquid-feeding tubes being fixed on same straight line using fix bar so that multiple strip hydrophilic regions
Immune response can be carried out simultaneously, ensure that Parallel testing, so as to reduce operating error.
4th, the delivery port of liquid-adding device has hydrophilic coating so that and the manufacture material of liquid-adding device is not limited to water wetted material,
And the relatively inexpensive hydrophobic materials such as polypropylene can be used, liquid-adding device or its subassembly is made as disposable apparatus
With, avoid cleaning instrument trouble.
Brief description of the drawings
Fig. 1 is the film top view in the utility model automatic detector；
Fig. 2 is the utility model automatic detector side sectional view；
In all of the figs, identical reference is used for representing identical element or structure, wherein：1- Western blottings
Film, 11- hydrophobic regions, 12- strip hydrophilic regions, 13- lands, 21- liquid-feeding tubes, 23- fix bars, 3- bases.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only explaining
The utility model, it is not used to limit the utility model.In addition, institute in each embodiment of the utility model disclosed below
As long as the technical characteristic being related to does not form conflict each other, can is mutually combined.
The utility model provides a kind of automatic detector for Western blotting, including base 3, Western blotting film 1,
Liquid-adding device and waste collecting device etc.；
The Western blotting film 1 is arranged at the top of base 3, and the surface of Western blotting film 1 has with hydrophobic region
Multiple parallel strip hydrophilic regions 12 at 11 intervals, each strip hydrophilic region 12 have one or more lands 13, institute
Stating has target antigen on land 13, for being combined with the target antibody in solution to be measured, as shown in figure 1, each bar
Shape hydrophilic region 12 is respectively used to combine different solution to be measured, so as to which the target independently carried out in different solution to be measured resists
The immune-blotting method of body；
The liquid-adding device have with the multiple liquid feeding passages correspondingly of strip hydrophilic region 12, the liquid feeding passage
Delivery port is oppositely arranged with corresponding strip hydrophilic region 12, and distance is 0.1mm~2mm, for adding to strip hydrophilic region 12
Add reaction solution, the water inlet of one end connection waste collecting device of the strip hydrophilic region 12, the waste collecting device is used
In collecting unnecessary reaction solution, the reaction solution includes solution to be measured, buffer solution, two corresponding anti-solution or chromophoric solution.
Wherein, the material of the delivery port of the liquid-adding device is water wetted material, with adsorption reaction liquid, is shaped as circular or ellipse
Circle, the internal diameter of the delivery port is 0.1mm~2mm, and external diameter is 0.5mm~5mm, is mutually fitted with the width with strip hydrophilic region
Should, because water has surface tension, even if the width of the external diameter of delivery port and strip hydrophilic region is slightly distinguished, water also can be complete
Cover the width of strip hydrophilic region；The material of the delivery port of the liquid-adding device is water wetted material, with adsorption reaction liquid,
Simultaneously as the delivery port of liquid-adding device is 0.1mm~2mm with the distance of corresponding strip hydrophilic region 12, and both it is
Water wetted material, therefore spilling for reaction solution can be prevented and pollute adjacent strip hydrophilic region 12.
Liquid-adding device can use individually disposable liquid-feeding tube 21, and the water inlet of liquid-feeding tube 21 is above, delivery port
Positioned at lower section, the water inlet from top, you can add reaction solution to the strip hydrophilic region 12 relative with delivery port；
Liquid-adding device can also be made up of multiple liquid-feeding tubes 21 and fluid filling pump, and the fluid filling pump has and multiple liquid-feeding tubes
Multiple delivery ports corresponding to 21, each delivery port of the fluid filling pump are connected with the water inlet of multiple liquid-feeding tubes 21, formed with
The multiple liquid feeding passages correspondingly of strip hydrophilic region 12, the water inlet of the water inlet of the fluid filling pump as the liquid-adding device
Mouthful, the delivery port of the delivery port of the liquid-feeding tube 21 as the liquid-adding device, fluid filling pump can use syringe pump, can also make
With peristaltic pump, flow peristaltic pump due to reaction solution, and the minimum 1 μ L/min of flow once can be inputted to multiple liquid-feeding tubes 21,
Compare saving reaction solution, maximum flow 1000mL/min, washed so as to quickly be passed through buffering, clean strip hydrophilic region
12, so as to the utility model that is particularly suitable for use in；When adding different types of reaction solution, the liquid-feeding tube of different inner diameters can be used instead
21, to adapt to experiment demand, such as when adding buffer solution, the liquid-feeding tube 21 of larger interior diameter can be used instead, by strip hydrophilic area
Domain 12 cleans up.
Liquid-adding device can also utilize the fix bar 23 with multiple fixed parts, and multiple liquid-feeding tubes 21 are fixed on together
On one straight line；Fixed part can be the fixed structures such as fixing hole, clip or the buckle of periphery for being sheathed on the liquid-feeding tube 21, such as
Shown in Fig. 2.
Liquid-adding device can use the relatively inexpensive materials such as polypropylene, conveniently make the sub-portion of liquid-adding device or liquid-adding device
Part uses as disposable apparatus, avoids the trouble of cleaning instrument；When liquid-adding device uses a hydrophobic material, can incite somebody to action
Delivery port is covered with hydrophilic coating, such as polyvinylpyrrolidone, poly- N- vinyl butyrate lactams, poly-N-vinylcaprolactam, poly-
Vinyl alcohol, polyvinyl alcohol derivative, polyurethane or modified poly ethylene etc..
In order to promote the relative motion of reaction solution and strip hydrophilic region 12, liquid-adding device can be moved, makes the liquid feeding
The delivery port of passage moves back and forth along strip hydrophilic region 12；The both sides of liquid-adding device, linear electric motors, straight line can be set respectively
The control terminal connection liquid-adding device of motor, the speed of linear electric motors typically set 5cm/s~below 15cm/s, the straight-line electric of both sides
Machine is run simultaneously；, still can be slow by liquid-adding device by solution to be measured while linear electric motors movement liquid-adding device motion
Make an addition to the surface of strip hydrophilic region 12, to prevent liquid evaporation, using keep the liquid level on strip hydrophilic region 12 as
0.1mm~1mm.
As it will be easily appreciated by one skilled in the art that better embodiment of the present utility model is the foregoing is only, and
Not to limit the utility model, any modification of all made within spirit of the present utility model and principle, equivalent substitution and
Improve etc., it should be included within the scope of protection of the utility model.
Claims (8)
1. a kind of liquid-adding device for Western blotting, it is characterised in that the liquid-adding device has one or more water inlets
And multiple delivery ports, the material of the delivery port is water wetted material, is shaped as circular or ellipse, the internal diameter of the delivery port
For 0.1mm~2mm, external diameter is 0.5mm~5mm.
2. liquid-adding device as claimed in claim 1, it is characterised in that described including multiple liquid-feeding tubes (21) and fluid filling pump
Fluid filling pump has multiple delivery ports corresponding with multiple liquid-feeding tubes (21), each delivery port and the multiple liquid-feeding tubes of the fluid filling pump
(21) water inlet is connected, and forms multiple liquid feeding passages, the water inlet of the water inlet of the fluid filling pump as the liquid-adding device
Mouthful, the delivery port of the delivery port of the liquid-feeding tube (21) as the liquid-adding device.
3. liquid-adding device as claimed in claim 2, it is characterised in that the fluid filling pump is syringe pump or peristaltic pump.
4. liquid-adding device as claimed in claim 2, it is characterised in that the liquid-adding device also includes fix bar (23), described
Fix bar (23) has multiple fixed parts, for multiple liquid-feeding tubes (21) to be fixed on into same straight line.
5. liquid-adding device as claimed in claim 4, it is characterised in that the fixed part is corresponding with the liquid-feeding tube (21)
Fixing hole, the fixing hole is sheathed on the periphery of the liquid-feeding tube (21).
6. liquid-adding device as claimed in claim 2, it is characterised in that the liquid-feeding tube (21) is water wetted material.
7. liquid-adding device as claimed in claim 1, it is characterised in that the delivery port of the liquid-adding device has hydrophilic coating.
8. liquid-adding device as claimed in claim 7, it is characterised in that the hydrophilic coating is polyvinylpyrrolidone, poly- N-
Vinyl butyrate lactam, poly-N-vinylcaprolactam, polyvinyl alcohol, polyvinyl alcohol derivative, polyurethane or modified poly ethylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720304742.2U CN206696297U (en) | 2017-03-27 | 2017-03-27 | A kind of liquid-adding device for Western blotting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720304742.2U CN206696297U (en) | 2017-03-27 | 2017-03-27 | A kind of liquid-adding device for Western blotting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206696297U true CN206696297U (en) | 2017-12-01 |
Family
ID=60444046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720304742.2U Expired - Fee Related CN206696297U (en) | 2017-03-27 | 2017-03-27 | A kind of liquid-adding device for Western blotting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206696297U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107045067A (en) * | 2017-03-27 | 2017-08-15 | 武汉优视科技有限公司 | A kind of liquid-adding device for Western blotting |
-
2017
- 2017-03-27 CN CN201720304742.2U patent/CN206696297U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107045067A (en) * | 2017-03-27 | 2017-08-15 | 武汉优视科技有限公司 | A kind of liquid-adding device for Western blotting |
| CN107045067B (en) * | 2017-03-27 | 2020-07-03 | 武汉优视科技有限公司 | Liquid adding device for immunoblotting |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171201 |
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| CF01 | Termination of patent right due to non-payment of annual fee |