CN206675745U - A kind of efficient quick bactericidal unit of Plant Tissue Breeding utensil - Google Patents

Abstract

It the utility model is related to field of plant tissue culture technique, more particularly to a kind of efficient quick bactericidal unit of Plant Tissue Breeding utensil, including frame, sterilization chamber, cooling chamber, conveyer belt, dispensing port, collect mouth, steam generator, electric heating accessory system and control system；The sterilization chamber, cooling chamber are arranged in frame, and the conveyer belt passes through inside sterilization chamber and cooling chamber, and described conveyer belt one end is provided with dispensing port, and the other end, which is provided with, collects mouth；The steam generator is connected by steam conveying pipe and steam jet with sterilization chamber；The beneficial effects of the utility model are：The bactericidal unit employs superheated steam, further heated on the basis of common steam, and then the effect of higher temperature shorter time sterilizing, simultaneously, device need not be closed, continuous operation can be achieved, be provided with conveyer and cooling device, in high volume continuously tissue culturing vessel can be sterilized.

Description

An efficient and rapid sterilization device for plant tissue culture equipment

technical field

The utility model relates to the field of tissue culture, in particular to an efficient and rapid sterilizing device for plant tissue culture equipment.

Background technique

In the work of plant tissue culture, the thorough sterilization of medium and utensils and correct aseptic operation are the key to prevent bacterial infection and ensure the normal growth and differentiation of tissue culture materials, which is the most basic and most important in tissue culture work. technology. Scientists engaged in tissue culture work need to sterilize, disinfect and sterilize the utensils used for plant tissue culture. The so-called sterilization refers to the use of physical or chemical methods to kill all microorganisms on the surface and inside of objects.

Autoclaving refers to the use of heat energy to kill microorganisms. The principle is that the high temperature denatures the protein in the protoplasm, inactivates the enzyme, and causes the death of the microorganism. High temperature sterilization is the most commonly used sterilization method in plant tissue culture, mainly including high temperature steam sterilization, dry heat sterilization and burning sterilization.

High-temperature steam sterilization belongs to damp heat sterilization. Since damp heat sterilization has latent heat, the temperature of the object to be sterilized is lower than that of steam. The steam condenses into water on its surface and releases latent heat, which can rapidly increase the temperature of the object to be sterilized. And because water and steam have larger heat capacity, faster heat conduction, and stronger penetrating power than air, they require lower temperature and shorter time than dry heat sterilization.

At present, the commonly used sterilization device is an autoclave. The problems that are likely to exist during the use of an autoclave are: exhaustion is not complete, and the domestic cold air is not exhausted before boosting, resulting in a lower actual temperature in the pot. ;The accumulation of items in the disinfection cylinder is too tight and full, which hinders the circulation of steam and heat exchange, slows down the temperature rise in the container, and causes incomplete sterilization inside the object; the volume of the sterilized object is too large, and high-temperature steam sterilization Usually at 121 ° C for 15 minutes. This regulation is limited to the small-capacity culture medium used in test tubes and triangular flasks. If the volume is too large, especially solid medium, the sterilization will not be complete, and the sterilization time should be extended appropriately; The temperature and pressure fluctuate.

Utility model content

The technical problem solved by the utility model is that in the prior art, the sterilizing device is prone to incomplete exhaust, too tight accumulation of articles in the sterilizing barrel, too large volume of sterilized objects, unstable temperature control, etc. Factors lead to incomplete sterilization and affect plant tissue culture.

In order to solve the above problems, we proposed a high-efficiency and rapid sterilization device for plant tissue culture equipment. The sterilization device uses superheated steam, which is further heated on the basis of ordinary steam, and then achieves higher temperature and shorter time sterilization At the same time, the device does not need to be airtight, and can realize continuous operation. It is equipped with a conveying device and a cooling device, and can continuously sterilize tissue culture vessels in large quantities.

In order to achieve the above needs, the utility model achieves the above objects through the following technical solutions: an efficient and rapid sterilization device for plant tissue culture equipment, including a frame, a sterilization chamber, a cooling chamber, a conveyor belt, a feeding port, and a receiving port , a steam generator, an electric heating auxiliary system and a control system; the sterilization chamber and the cooling chamber are all arranged on the frame, the conveyor belt passes through the sterilization chamber and the cooling chamber, and one end of the conveyor belt is provided with a feeding port, The other end is provided with a collection port; the steam generator communicates with the sterilization chamber through a steam delivery pipe and a steam nozzle;

The control system is connected with the conveyor belt, the steam generator, and the electric heating auxiliary system;

The sterilization chamber and the cooling chamber are tunnels with open ends of a cuboid, and steam isolation plates are arranged at both ends;

The electric heating auxiliary system includes a controller, an infrared heating device and an electric heating wire, and the electric heating wire is arranged in the steam conveying pipeline; the infrared heating device is arranged above the conveyor belt and at the top of the sterilization chamber.

Furthermore, a high-efficiency and rapid sterilization device for plant tissue culture equipment, a temperature sensor is arranged on the top of the sterilization chamber, and the temperature sensor is connected to a control system.

Further, an efficient and rapid sterilizing device for plant tissue culture equipment, the conveyor belt is provided with a frequency converter, and the frequency converter is connected to a control system.

Further, an efficient and rapid sterilizing device for plant tissue culture equipment, the cooling chamber is provided with windows, and the windows are provided in two groups, respectively located on both sides of the cooling chamber.

Further, a high-efficiency and rapid sterilization device for plant tissue culture equipment, the bottom of the sterilization chamber and the cooling chamber is provided with a condensed water return tank, and the bottom of the condensed water return tank is provided with a valve.

The utility model provides a high-efficiency and rapid sterilization device for plant tissue culture equipment. During use, the vessel to be sterilized is prevented from entering the feeding port, and the vessel enters the sterilization chamber with the movement of the conveyor belt. The sterilization chamber There is a high-temperature steam nozzle in the middle, which can directly spray high-temperature steam onto the surface of the vessel, and the steam pressure is 0.4MPa. At the same time, the electric heating wire set in the steam pipe can further heat the steam, so that the steam can reach a high temperature of 150-160°C; direct action On the vessel, at the same time, an infrared heating device is installed on the top of the sterilization chamber, which can instantly raise the surface temperature of the vessel to above 160°C; rapid sterilization can be completed in a short time, which improves the efficiency. The motor of the conveyor belt is connected with the frequency converter, which can adjust the time for the containers to pass through the sterilization chamber to realize the sterilization of different containers.

The sterilization chamber is equipped with a temperature sensor, which can realize real-time feedback of the temperature; the cooling chamber is equipped with a window, which can observe the condition of the vessel or medium during the sterilization process, and at the same time, the bottom of the sterilization chamber and the cooling chamber is equipped with a condensate return tank. The condensed water after sterilization can be recycled, saving resources.

The beneficial effect of the utility model is that: the sterilizing device adopts superheated steam, which is further heated on the basis of ordinary steam, and then achieves the effect of higher temperature and shorter time sterilization, and at the same time, the device does not need to be airtight, and can realize continuous operation , equipped with a conveying device and a cooling device, it can continuously sterilize tissue culture vessels in large quantities.

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Apparently, the drawings in the following description are some embodiments of the present utility model, and those skilled in the art can obtain other drawings according to these drawings without any creative effort.

Fig. 1 is the structural representation of present embodiment;

In the figure, frame 1, sterilization chamber 2, cooling chamber 3, conveyor belt 4, delivery port 5, collection port 6, steam generator 7, electric heating auxiliary system 8, control system 9, steam isolation board 21, temperature sensor 22, Condensed water return tank 23, valve 24, window 31, steam nozzle 71, steam delivery pipe 72, controller 81, infrared heating device 82, heating wire 83.

detailed description

In order to make the purpose, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Apparently, the described embodiments are some of the embodiments of the present utility model, but not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Example 1:

An efficient and rapid sterilizing device for plant tissue culture equipment, comprising a frame 1, a sterilizing chamber 2, a cooling chamber 3, a conveyor belt 4, a feeding port 5, a collecting port 6, a steam generator 7, an electric heating auxiliary system 8 and a control System 9; the sterilization chamber 2 and the cooling chamber 3 are all arranged on the frame 1, the conveyor belt 4 passes through the sterilization chamber 2 and the cooling chamber 3, and one end of the conveyor belt 4 is provided with a feeding port 5, and the other One end is provided with a collection port 6; the steam generator 7 communicates with the sterilization chamber 2 through a steam delivery pipe 72 and a steam nozzle 71;

The control system 9 is connected with the conveyor belt 4, the steam generator 7, and the electric heating auxiliary system 8;

The sterilization chamber 2 and the cooling chamber 3 are tunnels open at both ends of a cuboid, and steam isolation plates 21 are arranged at both ends;

The electric auxiliary system 8 includes a controller 81, an infrared heating device 82 and an electric heating wire 83, and the electric heating wire 83 is arranged in the steam delivery pipeline 72; .

A temperature sensor 22 is arranged on the top of the sterilization chamber 2 , and the temperature sensor 22 is connected with the control system 9 .

The conveyor belt 4 is provided with a frequency converter, and the frequency converter is connected with a control system 9 .

The cooling cavity 3 is provided with windows 31 , and the windows 31 are provided in two groups, respectively located on both sides of the cooling cavity 3 .

A condensed water return tank 23 is provided at the bottom of the sterilization chamber 2 and the cooling chamber 3 , and a valve 24 is provided at the bottom of the condensed water return tank 23 .

The high-efficiency and rapid sterilizing device for plant tissue culture equipment provided in this embodiment prevents the vessel to be sterilized from entering the insertion port during use, and the vessel enters the sterilization chamber along with the movement of the conveyor belt, and the sterilization chamber There is a high-temperature steam nozzle in the middle, which can directly spray high-temperature steam onto the surface of the vessel, and the steam pressure is 0.4MPa. At the same time, the electric heating wire set in the steam pipe can further heat the steam, so that the steam can reach a high temperature of 150-160°C; direct action On the vessel, at the same time, an infrared heating device is installed on the top of the sterilization chamber, which can instantly raise the surface temperature of the vessel to above 160°C; rapid sterilization can be completed in a short time, which improves the efficiency. The motor of the conveyor belt is connected with the frequency converter, which can adjust the time for the containers to pass through the sterilization chamber to realize the sterilization of different containers.

The sterilization chamber is equipped with a temperature sensor, which can realize real-time feedback of the temperature; the cooling chamber is equipped with a window, which can observe the condition of the vessel or medium during the sterilization process, and at the same time, the bottom of the sterilization chamber and the cooling chamber is equipped with a condensate return tank. The condensed water after sterilization can be recycled, saving resources.

The beneficial effect of this embodiment is that the sterilizing device uses superheated steam, which is further heated on the basis of ordinary steam, and then achieves the effect of higher temperature and shorter time sterilization. At the same time, the device does not need to be airtight and can realize continuous operation , equipped with a conveying device and a cooling device, it can continuously sterilize tissue culture vessels in large quantities.

Claims (5)

1. A high-efficiency and rapid sterilization device for plant tissue culture equipment, characterized in that: it includes a frame, a sterilization chamber, a cooling chamber, a conveyor belt, a delivery port, a collection port, a steam generator, an electric heating auxiliary system and a control system; Both the sterilization chamber and the cooling chamber are arranged on the frame, the conveyor belt passes through the sterilization chamber and the cooling chamber, one end of the conveyor belt is provided with a feeding port, and the other end is provided with a collection port; the steam generator It communicates with the sterilization chamber through steam delivery pipes and steam nozzles; The control system is connected with the conveyor belt, the steam generator, and the electric heating auxiliary system; The sterilization chamber and the cooling chamber are tunnels with open ends of a cuboid, and steam isolation plates are arranged at both ends; The electric heating auxiliary system includes a controller, an infrared heating device and an electric heating wire, and the electric heating wire is arranged in the steam conveying pipeline; the infrared heating device is arranged above the conveyor belt and at the top of the sterilization chamber. 2. The efficient and rapid sterilizing device for plant tissue culture apparatus according to claim 1, characterized in that: a temperature sensor is arranged on the top of the sterilizing chamber, and the temperature sensor is connected to a control system. 3. The high-efficiency and rapid sterilizing device for plant tissue culture apparatus according to claim 1, characterized in that: the conveyor belt is provided with a frequency converter, and the frequency converter is connected to a control system. 4. The high-efficiency and rapid sterilizing device for plant tissue culture apparatus according to claim 1, characterized in that: the cooling chamber is provided with windows, and the windows are provided with two groups, which are respectively located on both sides of the cooling chamber. 5. The high-efficiency and rapid sterilization device for plant tissue culture apparatus according to claim 1, characterized in that: the bottom of the sterilization chamber and the cooling chamber is provided with a condensed water return tank, and the bottom of the condensed water return tank is provided with a valve.