CN206594179U - A kind of novel drum type antibody incubation device - Google Patents
A kind of novel drum type antibody incubation device Download PDFInfo
- Publication number
- CN206594179U CN206594179U CN201720260733.8U CN201720260733U CN206594179U CN 206594179 U CN206594179 U CN 206594179U CN 201720260733 U CN201720260733 U CN 201720260733U CN 206594179 U CN206594179 U CN 206594179U
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- China
- Prior art keywords
- box
- roller
- incubation
- motor
- pvdf membrane
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- Expired - Fee Related
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- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The utility model discloses a kind of novel drum type antibody incubation device, including outer barrel, roller, it is incubated box and motor, the roller, incubation box and motor are inserted in outer barrel, the power transmission shaft of motor and the axle center of roller are connected, the state blocked between the cylinder outer wall and outer tube inner wall for close contact, it is characterised in that：The incubation box is round box shape shape, is connected on the inwall of roller, laying pvdf membrane in the incubation box, and antibody diluent is placed on pvdf membrane.Compared with tradition is incubated box, the utility model on the premise of antibody incubation effect is ensured, the usage amount of antibody reduce at least more than half, greatly saved experiment funds.Rotation of the utility model by roller at the uniform velocity so that contact of the pvdf membrane with antibody evenly, results in and is more preferably incubated efficiency.
Description
Technical field
The utility model is related to antibody incubation technical field, more particularly, is incubated on a kind of novel drum type antibody
Educate device.
Background technology
In life science, the western blot test of certain protein content in sample is detected (hereinafter referred to as
Western Blot are tested) it is common pilot project.Western Blot contain as certain specific protein in detection biological sample
The common technology of amount, is the common means of biologic test company.The antibody used in Western Blot processs of the test makees achievement
Height, price is extremely expensive, and researcher will spend a large amount of funds to be used to buy antibody every year.
Pvdf membrane is general in traditional Western Blot experiments is commonly being incubated incubation in box, at least with 4 milliliters
The antibody diluent of left and right can just cover the pvdf membrane after shearing.And potency can be significantly after used antibody diluent is reclaimed
Reduction, such waste can cause great economic loss, aggravate experimentation cost.
The content of the invention
Goal of the invention：In view of the shortcomings of the prior art, applicant improves by repeatedly practice, devises a kind of Novel rotary drum
Formula antibody incubation device, overcomes conventional antibodies and is incubated the defect that box largely expends antibody diluent.The couveuse is ensureing antibody
On the premise of being incubated effect, measured the need for can greatly reducing antibody, reduce the waste of research funding.
Technical scheme：In order to realize foregoing invention purpose, the technical scheme that the utility model is used for：A kind of new rolling
Cartridge type antibody incubation device, including outer barrel, roller, incubation box and motor, the roller, incubation box and motor are inserted in outer barrel,
The power transmission shaft of motor and the axle center of roller are connected, to be in close contact the state blocked between the cylinder outer wall and outer tube inner wall,
It is characterized in that：The incubation box is round box shape shape, is connected on the inwall of roller, laying pvdf membrane in the incubation box,
Antibody diluent is placed on pvdf membrane.
Further, several can be placed in roller described in the utility model and is incubated box, you can be incubated multiple lists simultaneously
Position.
Yet further, preferred embodiment of the present utility model is：Laying pvdf membrane in the incubation box, pvdf membrane surface is put
Enter film fixator, the film fixator is that diameter is slightly less than an elastic circular ring for being incubated box internal diameter, can play guarantee PVDF
Film, which is followed, is incubated the effect that box is rotated together.
It may be preferred that motor described in the utility model is adjustable speed motor.
Beneficial effect：Compared with prior art, its advantage is the utility model：
1st, compared with tradition is incubated box, the utility model is on the premise of antibody incubation effect is ensured, the usage amount of antibody
Reduce at least more than half, greatly saved experiment funds.
2nd, rotation of the utility model by roller at the uniform velocity so that contact of the pvdf membrane with antibody evenly, is resulted in
More preferably it is incubated efficiency.
3rd, the whole incubating device small volume of the utility model, is easy to movement, can be placed in low temperature in refrigerator and be incubated.
Brief description of the drawings
Fig. 1 is a kind of structural representation of novel drum type antibody incubation device.
Fig. 2 is the schematic cross-section of incubation box in the utility model.
Embodiment
Below by a most preferred embodiment, the technical program is described in detail, but protection of the present utility model
Scope is not limited to the embodiment.
As shown in figure 1, a kind of novel drum type antibody incubation device, including outer barrel 4, roller 2, incubation box 3 and motor 1, institute
State roller 2, incubation box 3 and motor 1 to insert in outer barrel 4, the power transmission shaft of motor 1 is connected with the axle center of drum 2, the roller 2
The state blocked between outer wall and the inwall of outer barrel 4 for close contact.The incubation box 3 is round box shape shape, is connected to the interior of roller 2
On wall.Several can be placed in the roller 2 and is incubated box 3.
As shown in Fig. 2 placing antibody diluent 6 on laying pvdf membrane 5, pvdf membrane 5 in the incubation box 3.It is described to be incubated
Film fixator is inserted on laying pvdf membrane 5 in box 3, the surface of pvdf membrane 5, and the film fixator is that diameter is slightly less than the incubation internal diameter of box 3
An elastic circular ring.
The motor 1 is adjustable speed motor.
Described above is only preferred embodiment of the present utility model, it should be pointed out that：For the common skill of the art
For art personnel, on the premise of the utility model principle is not departed from, some improvements and modifications can also be made, these improve and
Retouching also should be regarded as protection domain of the present utility model.
Claims (4)
1. a kind of novel drum type antibody incubation device, including outer barrel (4), roller (2), incubation box (3) and motor (1), the rolling
Cylinder (2), incubation box (3) and motor (1) are inserted in outer barrel (4), and the power transmission shaft of motor (1) is connected with the axle center of roller (2), institute
State the state blocked between roller (2) outer wall and outer barrel (4) inwall for close contact, it is characterised in that：It is described incubation box (3) be
On round box shape shape, the inwall for being connected to roller (2), laid in the incubation box (3) and place anti-on pvdf membrane (5), pvdf membrane (5)
Body dilution (6).
2. a kind of novel drum type antibody incubation device according to claim 1, it is characterised in that：Can in the roller (2)
Place several and be incubated box (3).
3. a kind of novel drum type antibody incubation device according to claim 1, it is characterised in that：In the incubation box (3)
Pvdf membrane (5) is laid, film fixator is inserted on pvdf membrane (5) surface, and the film fixator is that diameter is slightly less than incubation box (3) internal diameter
An elastic circular ring.
4. a kind of novel drum type antibody incubation device according to claim 1, it is characterised in that：The motor (1) is can
Buncher.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720260733.8U CN206594179U (en) | 2017-03-17 | 2017-03-17 | A kind of novel drum type antibody incubation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720260733.8U CN206594179U (en) | 2017-03-17 | 2017-03-17 | A kind of novel drum type antibody incubation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206594179U true CN206594179U (en) | 2017-10-27 |
Family
ID=60123496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720260733.8U Expired - Fee Related CN206594179U (en) | 2017-03-17 | 2017-03-17 | A kind of novel drum type antibody incubation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206594179U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318678A (en) * | 2018-02-01 | 2018-07-24 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
| CN112903992A (en) * | 2021-02-04 | 2021-06-04 | 四川大学华西医院 | Efficient immunoblotting antibody incubation device and incubation method |
-
2017
- 2017-03-17 CN CN201720260733.8U patent/CN206594179U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318678A (en) * | 2018-02-01 | 2018-07-24 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
| CN112903992A (en) * | 2021-02-04 | 2021-06-04 | 四川大学华西医院 | Efficient immunoblotting antibody incubation device and incubation method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171027 Termination date: 20200317 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |