CN108318678A - A kind of rotary antibody incubation device of water wheels - Google Patents
A kind of rotary antibody incubation device of water wheels Download PDFInfo
- Publication number
- CN108318678A CN108318678A CN201810099488.6A CN201810099488A CN108318678A CN 108318678 A CN108318678 A CN 108318678A CN 201810099488 A CN201810099488 A CN 201810099488A CN 108318678 A CN108318678 A CN 108318678A
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- Prior art keywords
- skeleton
- ring skeleton
- vertical runner
- rotary
- reservoir
- Prior art date
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 238000011534 incubation Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims abstract description 13
- 239000012528 membrane Substances 0.000 claims abstract description 12
- 239000003292 glue Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 238000012546 transfer Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 238000003119 immunoblot Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to biomedical research equipment technical fields, more particularly to a kind of rotary antibody incubation device of water wheels, include the reservoir for containing confining liquid and the vertical runner in reservoir, several netted blades extended along its radial direction are installed on the vertical runner of water wheel type structure, two neighboring netted blade angulation is identical, and each net-veined leaf on piece is mounted on pvdf membrane.Vertical runner is arranged in the present invention, netted blade is set on the vertical runner, each net-veined leaf on piece is mounted on pvdf membrane, in vertical runner rotation process, pass through in the confining liquid that each netted blade for being mounted with pvdf membrane can be carried out of reservoir, confining liquid is covered on pvdf membrane, and then realize the transfer of protein, it is poured between the interior ring skeleton of vertical runner and outer ring skeleton by the water pump in reservoir by confining liquid is top-down simultaneously, confining liquid that flows from above to below is formed, the transfer of protein may be implemented in the confining liquid liquid of flowing.
Description
Technical field
The present invention relates to biomedical research equipment technical field, more particularly to a kind of rotary antibody incubation dress of water wheels
It sets.
Background technology
Immunoblot assay (immunoblotting) in field of biomedical research, Protocols in Molecular Biology is again
Claim Western blotting technique (Westem blotting), have been more and more widely used, it can effectively to sample to be tested into
Row quantitative and semi-quantitative protein analysis.It includes mainly 5 steps：Electrophoresis, transfer, closing, hybridization and colour developing, wherein sealing
It closes and is also referred to as antibody incubation process with hybrid process.In immunoblot experiment, protein electrophoresis and transferring film method are highly developed,
Also there are the research equipment and device of specification in the market.After target protein is transferred to transfer film, need to further it carry out and antibody
The total incubation of solution.Traditional incubation method is using hybridization bag or to be incubated box, and hybridization bag is also easy to produce bubble, causes in use
Antibody incubation is incomplete, and hybridization bag cracky brings the danger of pollution；Tradition is incubated the box that box is all general structure, confrontation
Body dosage is bigger, and in addition the size specification of transfer film is different, is difficult to prepare the applicable box of multiple phases in reality.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of rotary antibody incubation device of water wheels.
To solve the above problems, the present invention provides following technical scheme：
A kind of rotary antibody incubation device of water wheels includes to contain the reservoir of confining liquid and mounted on reservoir
Interior vertical runner is provided with to install the holder of vertical runner in reservoir, is equipped with to drive at the top of the holder
The driving motor of vertical runner rotation, the outer wall of reservoir are provided with control box, control circuit board and electricity are equipped in control box
Source, control circuit board are electrically connected with the power supply, and driving motor is electrically connected by conducting wire and control circuit board, peace outside control box
Equipped with the switch being electrically connected with control circuit board, vertical runner is water wheel type structure, on the vertical runner of the water wheel type structure
Several netted blades extended along its radial direction are installed, two neighboring netted blade angulation is identical, each net-veined leaf
On piece is mounted on pvdf membrane.
Preferentially, water pump is also equipped in reservoir, feed pipe is installed on holder, one end of feed pipe and water pump it is defeated
Outlet connects, and the other end of water pump extends upwardly to the surface at the top of vertical runner along holder, and water pump passes through conducting wire and control
Circuit board is electrically connected.
Preferentially, vertical runner includes turntable body and blade mounting bracket, is equipped with and driving at the center of turntable body
The axis hole of the output end connection of motor, blade mounting bracket includes interior ring skeleton, outer ring skeleton and closed-loop, interior ring skeleton and outer shroud
Skeleton is fixedly mounted on the homonymy of turntable body and is coaxially arranged, and the bottom of interior ring skeleton and appearance skeleton is and turntable
Ontology is fixedly connected, and all blades are installed between interior ring skeleton and outer ring skeleton, and outer ring skeleton is engraved structure, closed-loop
Inner ring edge for disc-shaped structure, closed-loop is connect with the top of interior ring skeleton, outer ring edge and the outer ring skeleton of closed-loop
Top connection.
Preferentially, the outer ring skeleton of engraved structure includes lower ring body, upper ring body and several connecting edges, lower ring body and upper
Ring body is arranged in parallel, and connecting edge is vertically connected between upper ring body and lower ring body, and lower ring body is educated turntable body and is fixedly connected, adjacent
Between two connecting edges distance it is equal and constitute for confining liquid by window.
Preferentially, it is equipped with inner annular recess on the inside of each connecting edge, which extends at the top of upper ring body,
Several outer annular recess are equipped on the outside of interior ring skeleton, institute's outer annular recess all extends to the top of interior ring skeleton, each outer annular recess
A corresponding inner annular recess simultaneously constitutes one group to install the slot of netted blade.
Preferentially, the inside of closed-loop is equipped with inner ring buckle and outer ring buckle, outer ring buckle and the outer ring skeleton of closed-loop
Upper ring body on the outside of be clasped, the inside top of interior ring skeleton is equipped with card slot, the inner ring buckle of closed-loop in interior ring skeleton
Card slot be clasped.
Preferentially, the bottom of interior ring skeleton and outer ring skeleton is fixedly connected by glue with turntable body.
Preferentially, closed-loop is the plastic part of transparent material.
Advantageous effect：Vertical runner is arranged, in the vertical runner in a kind of rotary antibody incubation device of water wheels of the present invention
The netted blade of upper setting, each net-veined leaf on piece are mounted on pvdf membrane, in vertical runner rotation process, are each mounted with PVDF
Pass through in the confining liquid that the netted blade of film can all be carried out of reservoir, confining liquid is covered on pvdf membrane, and then realizes
The transfer of protein, while by the water pump in reservoir by the top-down inner ring for pouring vertical runner of confining liquid
Between skeleton and outer ring skeleton, confining liquid that flows from above to below is formed, turning for protein may be implemented in the confining liquid liquid of flowing
Print.
Description of the drawings
Fig. 1 is the dimensional structure diagram one of the present invention；
Fig. 2 is the dimensional structure diagram two of the present invention；
Fig. 3 is the side view of the present invention；
Fig. 4 is the dimensional decomposition structure diagram of runner in the present invention；
Fig. 5 is the enlarged diagram at A in Fig. 4；
Reference sign：Reservoir 1, vertical runner 2, holder 3, driving motor 4, control box 5, netted blade 6, water pump
7, feed pipe 8, turntable body 9, interior ring skeleton 10, outer annular recess 10a, outer ring skeleton 11, upper ring body 11a, lower ring body 11b, even
Connect rib 11c, inner annular recess 11d, closed-loop 12.
Specific implementation mode
With reference to the accompanying drawings of the specification and embodiment, specific embodiments of the present invention are described in further detail：
Include the storage for containing confining liquid referring to figs. 1 to a kind of rotary antibody incubation device of water wheels shown in fig. 5
Liquid bath 1 and the vertical runner 2 in reservoir 1 are provided with to install the holder 3 of vertical runner 2 in reservoir 1, should
The top of holder 3 is equipped with the driving motor 4 for driving vertical runner 2 to rotate, and the outer wall of reservoir 1 is provided with control box 5,
Control circuit board and power supply are installed in control box 5, control circuit board is electrically connected with the power supply, driving motor 4 by conducting wire with
Control circuit board is electrically connected, and the switch being electrically connected with control circuit board is equipped with outside control box 5, and vertical runner 2 is water
Wheeled construction is equipped with several netted blades 6 extended along its radial direction on the vertical runner 2 of the water wheel type structure, adjacent
Two 6 angulations of netted blade are identical, and pvdf membrane is mounted on each netted blade 6.
It is also equipped with water pump 7 in reservoir 1, feed pipe 8 is installed on holder 3, one end of feed pipe 8 is defeated with water pump 7
Outlet connects, and the other end of water pump 7 extends upwardly to the surface at 2 top of vertical runner along holder 3, water pump 7 by conducting wire with
Control circuit board is electrically connected.
Vertical runner 2 includes turntable body 9 and blade mounting bracket, is equipped with and driving motor 4 at the center of turntable body 9
Output end connection axis hole, blade mounting bracket includes interior ring skeleton 10, outer ring skeleton 11 and closed-loop 12,10 He of interior ring skeleton
Outer ring skeleton 11 is fixedly mounted on the homonymy of turntable body 9 and is coaxially arranged, the bottom of interior ring skeleton 10 and appearance skeleton
Portion is fixedly connected with turntable body 9, and all blades are installed between interior ring skeleton 10 and outer ring skeleton 11, outer ring skeleton 11
For engraved structure, closed-loop 12 is disc-shaped structure, and the inner ring edge of closed-loop 12 is connect with the top of interior ring skeleton 10, is sealed
The outer ring edge of closed loop 12 is connect with the top of outer ring skeleton 11.
The outer ring skeleton 11 of engraved structure includes lower ring body 11b, upper ring body 11a and several connecting edges 11c, lower ring body
11b and upper ring body 11a are arranged in parallel, and connecting edge 11c is vertically connected between upper ring body 11a and lower ring body 11b, lower ring body 11b
Turntable body 9 is educated to be fixedly connected, between two neighboring connecting edge 11c distance it is equal and constitute for confining liquid by window.
Outer ring skeleton 11 is engraved structure, and netted blade 6 can be smoothly immersed in reservoir 1 in rotary course
Confining liquid, while also ensureing that confining liquid that flows from above to below enters between interior ring skeleton 10 and outer ring skeleton 11.
It is equipped with inner annular recess 11d on the inside of each connecting edge 11c, inner annular recess 11d extends to upper ring body 11a
Top, 10 outside of interior ring skeleton are equipped with several outer annular recess 10a, and institute outer annular recess 10a all extends to the top of interior ring skeleton 10
Portion, each outer annular recess 10a correspond to an inner annular recess 11d and constitute one group to install the slot of netted blade 6.
The inside of closed-loop 12 is equipped with inner ring buckle and outer ring buckle, outer ring buckle and the outer ring skeleton 11 of closed-loop 12
It is clasped on the outside of upper ring body 11a, the inside top of interior ring skeleton 10 is equipped with card slot, inner ring buckle and the inner ring bone of closed-loop 12
Card slot on frame 10 is clasped.
Closed-loop 12 is installed by the way of being clasped, and is convenient for changing netted blade 6.
The bottom of interior ring skeleton 10 and outer ring skeleton 11 is fixedly connected by glue with turntable body 9.
Closed-loop 12 is the plastic part of transparent material.
Advantageous effect：Vertical runner 2 is arranged, at this vertical turn in a kind of rotary antibody incubation device of water wheels of the present invention
Netted blade 6 is set on wheel 2, is mounted on pvdf membrane on each netted blade 6, it is each to fill in 2 rotation process of vertical runner
Being loaded with the netted blade 6 of pvdf membrane can all pass through out of reservoir 1 in the confining liquid of carrying, and confining liquid is covered in pvdf membrane
On, and then realize the transfer of protein, while being poured by the water pump 7 in reservoir 1 by confining liquid is top-down
Between the interior ring skeleton 10 and outer ring skeleton 11 of vertical runner 2, confining liquid that flows from above to below, the confining liquid liquid of flowing are formed
The transfer of protein may be implemented.
The above described is only a preferred embodiment of the present invention, not making any limit to the technical scope of the present invention
System, thus it is every according to the technical essence of the invention to any subtle modifications, equivalent variations and modifications made by above example,
In the range of still falling within technical scheme of the present invention.
Claims (8)
1. a kind of rotary antibody incubation device of water wheels, it is characterised in that：Include contain confining liquid reservoir (1) and
Vertical runner (2) in reservoir (1), reservoir (1) is interior to be provided with to install the holder (3) of vertical runner (2),
The driving motor (4) for driving vertical runner (2) to rotate, the outer wall setting of reservoir (1) are installed at the top of the holder (3)
There is control box (5), control circuit board and power supply are installed in control box (5), control circuit board is electrically connected with the power supply, driving electricity
Machine (4) is electrically connected by conducting wire and control circuit board, is equipped with outside control box (5) and is opened with what control circuit board was electrically connected
It closes, vertical runner (2) is water wheel type structure, is equipped on the vertical runner (2) of the water wheel type structure several along its radial direction
The netted blade (6) extended, two neighboring netted blade (6) angulation is identical, is mounted on each netted blade (6)
Pvdf membrane.
2. the rotary antibody incubation device of a kind of water wheels according to claim 1, it is characterised in that：In reservoir (1) also
Water pump (7) is installed, feed pipe (8) is installed on holder (3), one end of feed pipe (8) is connect with the output end of water pump (7),
The other end of water pump (7) extends upwardly to the surface at the top of vertical runner (2) along holder (3), and water pump (7) passes through conducting wire and control
Circuit board processed is electrically connected.
3. the rotary antibody incubation device of a kind of water wheels according to claim 2, it is characterised in that：Vertical runner (2) wraps
Turntable body (9) and blade mounting bracket have been included, has been equipped at the center of turntable body (9) and is connect with the output end of driving motor (4)
Axis hole, blade mounting bracket includes interior ring skeleton (10), outer ring skeleton (11) and closed-loop (12), interior ring skeleton (10) and outer shroud
Skeleton (11) is fixedly mounted on the homonymy of turntable body (9) and is coaxially arranged, interior ring skeleton (10) and appearance skeleton
Bottom is fixedly connected with turntable body (9), and all blades are installed between interior ring skeleton (10) and outer ring skeleton (11), outside
Ring skeleton (11) is engraved structure, and closed-loop (12) is disc-shaped structure, inner ring edge and the interior ring skeleton of closed-loop (12)
(10) top connection, the outer ring edge of closed-loop (12) is connect with the top of outer ring skeleton (11).
4. the rotary antibody incubation device of a kind of water wheels according to claim 3, it is characterised in that：The outer shroud of engraved structure
Skeleton (11) includes lower ring body (11b), upper ring body (11a) and several connecting edges (11c), lower ring body (11b) and upper ring body
(11a) is arranged in parallel, and connecting edge (11c) is vertically connected between upper ring body (11a) and lower ring body (11b), and lower ring body (11b) is educated
Turntable body (9) is fixedly connected, between two neighboring connecting edge (11c) distance it is equal and constitute for confining liquid by window
Mouthful.
5. the rotary antibody incubation device of a kind of water wheels according to claim 4, it is characterised in that：Each connecting edge
Inner annular recess (11d) is equipped on the inside of (11c), which extends at the top of upper ring body (11a), inner ring
Several outer annular recess (10a) are equipped on the outside of skeleton (10), institute outer annular recess (10a) all extends to the top of interior ring skeleton (10)
Portion, the corresponding inner annular recess (11d) of each outer annular recess (10a) simultaneously constitute one group to install the slot of netted blade (6).
6. the rotary antibody incubation device of a kind of water wheels according to claim 5, it is characterised in that：Closed-loop (12) it is interior
Side is equipped with inner ring buckle and outer ring buckle, and the outer ring of closed-loop (12) buckles on the outside of the upper ring body (11a) with outer ring skeleton (11)
Be clasped, the inside top of interior ring skeleton (10) is equipped with card slot, the inner ring buckle of closed-loop (12) in interior ring skeleton (10)
Card slot be clasped.
7. the rotary antibody incubation device of a kind of water wheels according to any one of claim 1~6, it is characterised in that：
The bottom of interior ring skeleton (10) and outer ring skeleton (11) is fixedly connected by glue with turntable body (9).
8. the rotary antibody incubation device of a kind of water wheels according to any one of claim 1~6, it is characterised in that：
Closed-loop (12) is the plastic part of transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810099488.6A CN108318678A (en) | 2018-02-01 | 2018-02-01 | A kind of rotary antibody incubation device of water wheels |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810099488.6A CN108318678A (en) | 2018-02-01 | 2018-02-01 | A kind of rotary antibody incubation device of water wheels |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108318678A true CN108318678A (en) | 2018-07-24 |
Family
ID=62891256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810099488.6A Pending CN108318678A (en) | 2018-02-01 | 2018-02-01 | A kind of rotary antibody incubation device of water wheels |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108318678A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203069602U (en) * | 2013-02-21 | 2013-07-17 | 郑州大学 | Immune coupler |
| CN105181972A (en) * | 2015-09-09 | 2015-12-23 | 大连医科大学 | Constant temperature antibody incubation box |
| CN205484376U (en) * | 2016-01-23 | 2016-08-17 | 贾庆安 | A box pendulous device is hatched to antibody |
| CN205643356U (en) * | 2016-04-29 | 2016-10-12 | 北京致成生物医学科技有限公司 | Box is hatched to portable antibody |
| CN206300955U (en) * | 2016-12-26 | 2017-07-04 | 兰州奥润环保设备有限公司 | Many diaphragm Western blot instrument |
| CN206594179U (en) * | 2017-03-17 | 2017-10-27 | 上海优久生物科技有限公司 | A kind of novel drum type antibody incubation device |
| CN208672642U (en) * | 2018-02-01 | 2019-03-29 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
-
2018
- 2018-02-01 CN CN201810099488.6A patent/CN108318678A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203069602U (en) * | 2013-02-21 | 2013-07-17 | 郑州大学 | Immune coupler |
| CN105181972A (en) * | 2015-09-09 | 2015-12-23 | 大连医科大学 | Constant temperature antibody incubation box |
| CN205484376U (en) * | 2016-01-23 | 2016-08-17 | 贾庆安 | A box pendulous device is hatched to antibody |
| CN205643356U (en) * | 2016-04-29 | 2016-10-12 | 北京致成生物医学科技有限公司 | Box is hatched to portable antibody |
| CN206300955U (en) * | 2016-12-26 | 2017-07-04 | 兰州奥润环保设备有限公司 | Many diaphragm Western blot instrument |
| CN206594179U (en) * | 2017-03-17 | 2017-10-27 | 上海优久生物科技有限公司 | A kind of novel drum type antibody incubation device |
| CN208672642U (en) * | 2018-02-01 | 2019-03-29 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of rotary antibody incubation device of water wheels |
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