CN105277385A - Positioning and quantifying sampler for solid-state anaerobic fermentation substrate - Google Patents
Positioning and quantifying sampler for solid-state anaerobic fermentation substrate Download PDFInfo
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- CN105277385A CN105277385A CN201410327332.0A CN201410327332A CN105277385A CN 105277385 A CN105277385 A CN 105277385A CN 201410327332 A CN201410327332 A CN 201410327332A CN 105277385 A CN105277385 A CN 105277385A
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Abstract
The invention discloses a positioning and quantifying sampler for a solid-state anaerobic fermentation substrate. The sampler comprises a positioning and sampling tube, a quantifying rod and a quantifying blocking cap, wherein depth scales are marked on the outer wall of the positioning and sampling tube; a cavity hemicone-shaped tube opening is formed in the front end of the positioning and sampling tube; an opening is formed in the tail part of the positioning and sampling tube, and threads are formed in the outer wall of the tail part; the quantifying rod is a cylinder and is arranged in the positioning and sampling tube; threads are formed in the outer wall of the cylinder, and quantifying scales are marked on the outer wall of the cylinder; a sealing ring is arranged at the front end of the quantifying rod and is connected with the inner wall of the positioning and sampling tube in a piston matching manner; one end of the quantifying blocking cap is in threaded connection with the threads in the tail part of the positioning and sampling tube; the tail end of the quantifying rod penetrates out from the quantifying blocking cap; and the quantifying rod is in threaded connection with the quantifying blocking cap. The sampler can realize deep sampling of a solid culture medium without introducing in air during sampling, so influence on fermentation effect is avoided. The sampler is reasonable in design, convenient to use, free of influence on fermentation effect, suitable for positioned and quantitative sampling of the anaerobic fermentation culture medium at different depths.
Description
Technical field
The present invention relates to a kind of fermentable sampling instrument, specifically a kind of positioning and quantitative solid anaerobic digestion matrix sampler detected for deep layer solid culture and matrix sampling.
Background technology
At present, solid fermentation is divided into aerobic solid-state fermentation and anaerobic solid-state fermentation.The material thickness of aerobic fermentation is relatively little, between 30 to 50 centimetres, but anaerobic solids fermentation stromal thickness sometimes can reach 2 meters and more than.When studying anaerobic culture medium different parts growth of microorganism situation and nutrient culture media utilization power, need to sample the culture of corresponding position.Traditional sampler cannot sample the solid medium of deep layer, or brings air into when sampling, and affects ferment effect.So during the fermentation of research anaerobic solids, need a kind of convenient and reasonable, structure simply and do not affect the sampler of ferment effect.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, and a kind of positioning and quantitative solid anaerobic digestion matrix sampler is provided.This sampler is reasonable in design, easy to use, do not affect ferment effect, be applicable to anaerobic solids nutrient culture media different depth location, quantitative sampling.
Realizing the object of the invention technical scheme is:
A kind of positioning and quantitative solid anaerobic digestion matrix sampler, comprises
Location stopple coupon, described its outer wall of location stopple coupon indicates deep calibration, and its front end is the cavity half cone bodily form mouth of pipe, and location stopple coupon afterbody opening, afterbody outer wall are provided with screw thread;
Quantitative bar, described quantitative bar is column, and be arranged in the stopple coupon of location, this column outer wall is screw thread and indicates quantitative scale; The front end of described quantitative bar is provided with O-ring seal, and O-ring seal is that piston type connects with the inwall of location stopple coupon;
Quantitative card cap, one end of described quantitative card cap is spirally connected with the tailing screw flight of location stopple coupon, and the end of quantitative bar passes positioning jamming cap, and quantitative bar and positioning jamming cap are spirally connected.
Also be provided with sampling spanner, described sampling spanner and the end of quantitative bar connect.
In the deep calibration of described location stopple coupon, zero graduation is in this one end of extreme direction before the stopple coupon of location; In quantitative scale on described quantitative bar, zero graduation is in this one end of quantitative bar end direction.
The end of described quantitative bar is hexagon cylinder shape.
This positioning and quantitative solid anaerobic digestion matrix sampler can sample the solid medium of deep layer, can not bring air into, thus avoid the situation affecting ferment effect when sampling.This sampler is reasonable in design, easy to use, do not affect ferment effect, be applicable to anaerobic solids nutrient culture media different depth location, quantitative sampling.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment.
In figure, 1. locate the quantitative bar 3. quantitative card cap 4. of stopple coupon 2. and sample spanner 5. O-ring seal.
Embodiment
Below in conjunction with drawings and Examples, content of the present invention is further elaborated, but is not limitation of the invention.
Embodiment:
With reference to Fig. 1, a kind of positioning and quantitative solid anaerobic digestion matrix sampler, comprises
Location stopple coupon 1, described its outer wall of location stopple coupon 1 indicates deep calibration, and its front end is the cavity half cone bodily form mouth of pipe, and location stopple coupon 1 afterbody opening, afterbody outer wall are provided with screw thread;
Quantitative bar 2, described quantitative bar 2 is column, and be arranged in the stopple coupon 1 of location, this column outer wall is screw thread and indicates quantitative scale; The front end of described quantitative bar 2 is provided with O-ring seal 5, and O-ring seal 5 is that piston type connects with the inwall of location stopple coupon 1;
Quantitative card cap 3, one end of described quantitative card cap 3 is spirally connected with the tailing screw flight of location stopple coupon 1, and the end of quantitative bar 2 passes positioning jamming cap 3, and quantitative bar 2 is spirally connected with positioning jamming cap 3.
Also be provided with sampling spanner 4, described sampling spanner 4 connects with the end of quantitative bar 2.
In the deep calibration of described location stopple coupon 1, zero graduation is in this one end of extreme direction before the stopple coupon 1 of location; In quantitative scale on described quantitative bar 2, zero graduation is in this one end of quantitative bar 2 end direction.
The end of described quantitative bar 2 is hexagon cylinder shape.
Concrete sampling process:
1) quantitative bar 2 and O-ring seal 5 are inserted in the stopple coupon 1 of location;
2) quantitative bar 2 is passed quantitative card cap 3, and quantitative card cap 3 is tightened on the afterbody of location stopple coupon 1;
3) quantitative bar 2 is rotated with sampling spanner 4, determine the zero graduation position of quantitative bar 2, now quantitatively the O-ring seal 5 of bar 2 is positioned at zero graduation place, stopple coupon 1 front end, location, and the zero graduation of quantitative bar 2 is concordant with quantitative card cap 3 upper end, O-ring seal 5 is two, and the object of setting prevents air from entering;
4) location stopple coupon 1 is inserted anaerobic solids nutrient culture media to desired depth, this degree of depth reads by location stopple coupon 1 outer wall scale, quantitative bar 2 is rotated by quantitative spanner 4, make the upwards turn of quantitative bar 2, until rotate required sample volume, sampling sample is volume required can be read by quantitative bar 2 high scale;
5) stopple coupon 1 will be located and extend downward insertion in nutrient culture media again, and make nutrient culture media enter in quantitative sampling pipe 1 from the quantitative sampling pipe 1 front end mouth of pipe;
6) location stopple coupon 1 is extracted nutrient culture media, thus obtain depthkeeping degree set in anaerobic culture medium, determine the sample of volume, achieve that anaerobic solids nutrient culture media different depth is located, quantitative sampling.
Claims (4)
1. a positioning and quantitative solid anaerobic digestion matrix sampler, is characterized in that, comprise
Location stopple coupon, described its outer wall of location stopple coupon indicates deep calibration, and its front end is the cavity half cone bodily form mouth of pipe, and location stopple coupon afterbody opening, afterbody outer wall are provided with screw thread;
Quantitative bar, described quantitative bar is column, and be arranged in the stopple coupon of location, this column outer wall is screw thread and indicates quantitative scale; The front end of described quantitative bar is provided with O-ring seal, and O-ring seal is that piston type connects with the inwall of location stopple coupon;
Quantitative card cap, one end of described quantitative card cap is spirally connected with the tailing screw flight of location stopple coupon, and the end of quantitative bar passes positioning jamming cap, and quantitative bar and positioning jamming cap are spirally connected.
2. positioning and quantitative solid anaerobic digestion matrix sampler according to claim 1, is characterized in that, be also provided with sampling spanner, and described sampling spanner and the end of quantitative bar connect.
3. positioning and quantitative solid anaerobic digestion matrix sampler according to claim 1, is characterized in that, in the deep calibration of described location stopple coupon, zero graduation is in this one end of extreme direction before the stopple coupon of location; In quantitative scale on described quantitative bar, zero graduation is in this one end of quantitative bar end direction.
4. positioning and quantitative solid anaerobic digestion matrix sampler according to claim 1, is characterized in that, the end of described quantitative bar is hexagon cylinder shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410327332.0A CN105277385A (en) | 2014-07-10 | 2014-07-10 | Positioning and quantifying sampler for solid-state anaerobic fermentation substrate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410327332.0A CN105277385A (en) | 2014-07-10 | 2014-07-10 | Positioning and quantifying sampler for solid-state anaerobic fermentation substrate |
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| CN105277385A true CN105277385A (en) | 2016-01-27 |
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| CN201410327332.0A Pending CN105277385A (en) | 2014-07-10 | 2014-07-10 | Positioning and quantifying sampler for solid-state anaerobic fermentation substrate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834102A (en) * | 2016-12-22 | 2017-06-13 | 中国科学院宁波城市环境观测研究站 | Cut glue formula tubulose anaerobe and be separately cultured device and the anaerobe cultural method using the device |
| CN112592819A (en) * | 2021-01-26 | 2021-04-02 | 广州万为通达科技有限公司 | Biological fermentation condition detection device during biological fermentation |
| CN114456909A (en) * | 2022-03-17 | 2022-05-10 | 广东工业大学 | Deep-sea microorganism separation culture device and culture method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5996426A (en) * | 1996-10-25 | 1999-12-07 | Merck & Co., Inc. | End-sampling thief probe |
| US6631650B1 (en) * | 2000-07-31 | 2003-10-14 | Geneva Pharmaceuticals, Inc. | Thief sampling probe |
| CN201100855Y (en) * | 2007-11-26 | 2008-08-13 | 上海源耀生物科技有限公司 | A solid anaerobic ferment sampler |
| CN201229257Y (en) * | 2008-07-25 | 2009-04-29 | 泸州老窖股份有限公司 | Solid-state spirit cellar sampling apparatus |
| CN201876372U (en) * | 2010-11-25 | 2011-06-22 | 泸州品创科技有限公司 | Vinasse sampler |
| CN204255674U (en) * | 2014-07-10 | 2015-04-08 | 广西师范大学 | A kind of positioning and quantitative solid anaerobic digestion matrix sampler |
-
2014
- 2014-07-10 CN CN201410327332.0A patent/CN105277385A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5996426A (en) * | 1996-10-25 | 1999-12-07 | Merck & Co., Inc. | End-sampling thief probe |
| US6631650B1 (en) * | 2000-07-31 | 2003-10-14 | Geneva Pharmaceuticals, Inc. | Thief sampling probe |
| CN201100855Y (en) * | 2007-11-26 | 2008-08-13 | 上海源耀生物科技有限公司 | A solid anaerobic ferment sampler |
| CN201229257Y (en) * | 2008-07-25 | 2009-04-29 | 泸州老窖股份有限公司 | Solid-state spirit cellar sampling apparatus |
| CN201876372U (en) * | 2010-11-25 | 2011-06-22 | 泸州品创科技有限公司 | Vinasse sampler |
| CN204255674U (en) * | 2014-07-10 | 2015-04-08 | 广西师范大学 | A kind of positioning and quantitative solid anaerobic digestion matrix sampler |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834102A (en) * | 2016-12-22 | 2017-06-13 | 中国科学院宁波城市环境观测研究站 | Cut glue formula tubulose anaerobe and be separately cultured device and the anaerobe cultural method using the device |
| CN106834102B (en) * | 2016-12-22 | 2019-08-02 | 中国科学院宁波城市环境观测研究站 | It cuts glue formula tubulose anaerobe and is separately cultured device and the anaerobe cultural method using the device |
| CN112592819A (en) * | 2021-01-26 | 2021-04-02 | 广州万为通达科技有限公司 | Biological fermentation condition detection device during biological fermentation |
| CN112592819B (en) * | 2021-01-26 | 2024-06-04 | 佛山市广古调味品有限公司 | Biological fermentation condition detection device during biological fermentation |
| CN114456909A (en) * | 2022-03-17 | 2022-05-10 | 广东工业大学 | Deep-sea microorganism separation culture device and culture method |
| CN114456909B (en) * | 2022-03-17 | 2022-09-06 | 广东工业大学 | Deep-sea microorganism separation culture device and culture method |
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